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A study of the trichomes types and distribution and pollen morphology was carried out in nine Micromeria taxa (M. cristata ssp. cristata, M. cristata ssp. kosaninii, M. croatica, M. graeca ssp. graeca, M. graeca ssp. fruticulosa, M. juliana, M. kerneri, M. longipedunculata and M. microphylla) and five closely related Clinopodium species (C. dalmaticum, C. frivaldszkyanum, C. pulegium, C. serpyllifolium and C. thymifolium) from the Lamiaceae family of the Balkan Peninsula. By scanning electron microscope, non-glandular trichomes, peltate and capitate trichomes were observed on the calyx, leaves and stem of the studied species. Two subtypes of capitate trichomes were observed in Micromeria species: subtype 1 (consisting of a basal epidermal cell and an elliptically shaped head cell) and subtype 2 (consisting of a basal epidermal cell, two to three stalk cells and a round head cell). In Clinopodium species, three types of capitate trichomes were observed: subtype 1, subtype 3 (consisting of a basal epidermal cell, a short peduncle cell, and a single round head cell), and subtype 4 (consisting of a basal epidermal cell, a stalk cell, and an elongated head cell). These results support the recent transfer of Micromeria species from the section Pseudomelissa to the genus Clinopodium.

Introduction Peroral endoscopic myotomy (POEM) has been considered as a minimal-invasive, innovative technique for long-term treatment of all types of esophageal achalasia and other esophageal motility disorders. Patients and methods We report on 20 consecutive Greek patients with manometrically proved esophageal achalasia (14 patients with type I, 4 with type II, 2 with type III, and 4 with sigmoid esophagus), with an age range of 32–92 years, mean age 59 years, 12 males, successfully treated by POEM from 2013 to 2015. The Eckardt score was 7–12 (type III). Seventeen (85%) POEM procedures were performed in the Endoscopy Department, according to a previous study. During POEM, CO2 insufflation was mandatory, while the Triangle Tip knife was the only knife used in all procedures. Eckardt score, esophagogram and manometry before and after performing POEM were used for evaluation of our results. The follow-up period was 6 months to 3 years. Results Selective circular myotomy, 10–13 cm in length, was successfully completed in all patients without severe acute or late complications. Three patients (15%) showed moderate pneumomediastinum and pneumoperitoneum, which was successfully managed by abdominal needle drainage during the procedure. One patient showed mild pleural collection, and in one patient the clip–endoloop technique was used to successfully close the mucosal entry after the completion of POEM. The outcome was uneventful without any further clinical consequences. No other short- or long-term serious complications were reported. Patients were discharged after 1–3 days of hospitalization. Six months to 3 years after the POEM procedure, all patients were alive; the majority (90%) had complete clinical improvement, while two patients with sigmoid-type achalasia showed moderate-to-significant clinical improvement. Erosive esophagitis was reported in 15%. Conclusion Our results are in accordance with international data, and proved the safety and efficacy of the POEM technique for radical long-term treatment of all types of achalasia, including end-stage sigmoid-type achalasia, in the Endoscopy Department. However, long-term follow-up is necessary and awaited., Video abstract

Gottschlich G., Melikoki K., Eleftheriadou E. & Theodoropoulos K.: Three new Hieracium taxa (Asteraceae, Cichorieae) from Mt Cholomon, Chalkidiki, Central Makedonia, Greece. — Willdenowia 43: 59–64. June 2013. — Online ISSN 1868-6397; © 2013 BGBM Berlin-Dahlem. Stable URL: http://dx.doi.org/10.3372/wi.43.43106 Three new subspecies, Hieracium sparsum subsp. cholomonense, H. transiens subsp. levimaculatum and H. bohatschianum subsp. onosmoidiforme, all from Mt Cholomon, Chalkidiki, Central Makedonia, Greece, are described and illustrated.

Owing to the difficulties in measuring daily reference potential evapotranspiration, its estimation by means of the ASCE Penman-Monteith approach and various other empirical equations that are less demanding in terms of input data is favoured for various applications in vegetation science and climatology. This study presents the development of a Visual Basic software that can be used for the estimation of the daily PETref by means of the standardized ASCE Penman-Monteith equation for both short (clipped grass) and tall (full-cover alfalfa) crops and thirteen additional empirical equations. Statistical measures of goodness of fit are also calculated to make it easier for users to compare and detect the empirical equations that have the minimum bias of estimation against the ASCE Penman-Monteith equation.

Management of transboundary water resources has been the focus of many studies due to the observed water stress all over the globe combined with the continually increasing water demand. States sharing water resources often proceed to negotiations regarding water allocation issues and other managerial issues in order to settle their disputes. During such negotiations the use of appropriate tools, which will provide assistance to the opponent parties in terms of scenarios quantification and simulation processes, is a vital component toward conflict resolution. The present paper implements game theoretical concepts in the case study of Greek–Bulgarian negotiations on Nestos/Mesta transboundary river. The quantification of consequences caused by water flow decrease for different scenarios provides the background for estimations of compromising solutions that could be acceptable to both countries. Moreover, implementing interconnected games widens the countries’ available options and contributes to the avoidan...

Management of trans-boundary river basins is a major issue that has attracted great attention in recent years. The European Water Framework Directive (WFD) recommends management at a river basin level, overlooking any national or administrative borders. This new managerial approach impels water managers to disregard the trans-boundary nature of the water resources while considering an integrated river basin where only geographical boundaries exist. The new challenge for scientists and water managers is the establishment of water agreements between countries sharing water resources. These agreements should aim at the settlement of tensions and conflicts while providing the essential framework for cooperation and consensus building. Apparently, the content of these agreements should comply with international law and the relevant international conventions especially, as noted by the WFD, the UNECE Convention on the Protection and Use of Trans-boundary Watercourses and International Lakes (known as the Helsinki Rules), approved by the European Council in 1995. This paper examines the efficiency of water agreements and their precedent negotiations using the best known international examples while focusing on the Greek–Bulgarian agreement for the waters of the Nestos/Mesta River and its compliance with the WFD and the Helsinki Rules. As shown, the two countries have failed to implement a joint effort to put it into action; hence a methodological framework is proposed including certain strategic steps that can guide the two countries to a more effective and applicable water agreement taking into account the peculiarities of this trans-boundary area.

Border areas comprise inevitably problematic fields especially when the countries are bound to share common water resources. The conflict potential is rather high and quite often countries are directed to the negotiation table in order to form tranboundary agreements. These agreements aim to the settlement of tensions and conflicts originating from the management of shared water resources. Often a starting point of the conflict resolution is the water allocation scheme between the interested parts. The definition of entitlements over shared water resources in not an easy task and it requires the use of specific decision tools in order to reach the maximum level of objectivity. According to the Water Framework Directive (WFD) 2000/60, management of transboundary catchments shall be based on existing structures set by international agreements, such as the UN Convention on the Protection and Use of Transboundary Watercourses and International Lakes. The main aim of this paper is the implementation of a water allocation tool based on representative indicators characterizing the involved parties while placing emphasis on environmental protection. The variables used are proposed by the UN Convention on the Law of the Non-navigational Uses of International Watercourses incorporating both natural and socio-economic aspects. These factors are quantified for each country and a weight factor is assigned to each one depending on its importance and suitability for describing the environmental and socio-economic situation in each country. As an illustration example the transboundary river Nestos/Mesta case is presented.

The geographical distribution of the birch (BeTula pendula Roth.) in Greece is Limited to the northern mountains (Varnous, Voras, Vennio, Paiko, Beles, Orvilos, Lailias, Pangeo, Phalakro and Rhooope). The unique extensive pure Betula penduta - forest, which covers an area of about 1755 ha, occurs in the western part of the Rhodope mountain range. The other appearances of birch in the Greek area are limited either to small stands or to individuals in mixture with other forest trees. The Betula pendula forest in western Rhodope have been studied using the Braun - B1anquet mtethod with 36 phytosociological relevés. Two associations. Querco dalechampii - Betuletum pendulae (Quercetalia pubescentis) and Fago sylvalicae - Betuletum pendulae (Fagetali sylvAticae ), as well as rurther subdivisions, are distinguished. Their syntaxonomy as well as informatìon about the site characteristics, structure and syndynamical position of the distinguished communities are given.

The chemical composition of the essential oil (analysed by GC and GC–MS), the types and distribution of trichomes and pollen morphology (analysed by scanning electron microscopy) were investigated in two closely related species, Micromeria kerneri Murb. and Micromeria juliana (L.) Benth. (Lamiaceae) from Southeast Europe as a contribution to their taxonomy. The essential oil of M. kerneri was characterized by a high concentration of oxygenated sesquiterpenes, with caryophyllene-oxide as the major compound. Caryophyllene-oxide was also the major component of the essential oil of M. juliana from all localities, except from Mt Krivošije (Montenegro), where piperitone oxide was the major constituent. Non-glandular trichomes, peltate trichomes, and two types of capitate trichomes (type 1 composed of one basal epidermal cell, and one head cell with subcuticular space ; type 2 composed of one basal epidermal cell, two stalk cells, and one head cell with subcuticular space) were observed on leaves, the calyx and on the stem. Pollen of both species had six apertures (hexacolpate) set in the equatorial pollen belt (zonocolpate) and showed medium reticulate ornamentation. Multivariate analysis (PCA and UPGMA) of essential oil components clearly separated the investigated M. kerneri and M. juliana populations, and confirmed the opinion that they are different taxa. On the other hand, micromorphological traits between these species were the same. Nevertheless, definitive conclusions about the taxonomic relationships among these species will require genetic analysis.

A modern pollen deposition monitoring program has been initiated in Greece for the first time. For this purpose six modified Tauber traps (Hicks and Hyvarinen, 1986), were placed on a transect from black pine (Pinus nigra) forest to the tree-line of Scots pine (Pinus sylvestris) in the Pieria mountains.