90 results on '"Elena Iglesias"'
Search Results
2. Optimization of universal allogeneic CAR-T cells combining CRISPR and transposon-based technologies for treatment of acute myeloid leukemia
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Cristina Calviño, Candela Ceballos, Ana Alfonso, Patricia Jauregui, Maria E. Calleja-Cervantes, Patxi San Martin-Uriz, Paula Rodriguez-Marquez, Angel Martin-Mallo, Elena Iglesias, Gloria Abizanda, Saray Rodriguez-Diaz, Rebeca Martinez-Turrillas, Jorge Illarramendi, Maria C. Viguria, Margarita Redondo, Jose Rifon, Sara Villar, Juan J. Lasarte, Susana Inoges, Ascension Lopez-Diaz de Cerio, Mikel Hernaez, Felipe Prosper, and Juan R. Rodriguez-Madoz
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allogeneic CAR-T ,CRISPR ,transposon ,AML ,transcriptomics (RNA sequencing) ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Despite the potential of CAR-T therapies for hematological malignancies, their efficacy in patients with relapse and refractory Acute Myeloid Leukemia has been limited. The aim of our study has been to develop and manufacture a CAR-T cell product that addresses some of the current limitations. We initially compared the phenotype of T cells from AML patients and healthy young and elderly controls. This analysis showed that T cells from AML patients displayed a predominantly effector phenotype, with increased expression of activation (CD69 and HLA-DR) and exhaustion markers (PD1 and LAG3), in contrast to the enriched memory phenotype observed in healthy donors. This differentiated and more exhausted phenotype was also observed, and corroborated by transcriptomic analyses, in CAR-T cells from AML patients engineered with an optimized CAR construct targeting CD33, resulting in a decreased in vivo antitumoral efficacy evaluated in xenograft AML models. To overcome some of these limitations we have combined CRISPR-based genome editing technologies with virus-free gene-transfer strategies using Sleeping Beauty transposons, to generate CAR-T cells depleted of HLA-I and TCR complexes (HLA-IKO/TCRKO CAR-T cells) for allogeneic approaches. Our optimized protocol allows one-step generation of edited CAR-T cells that show a similar phenotypic profile to non-edited CAR-T cells, with equivalent in vitro and in vivo antitumoral efficacy. Moreover, genomic analysis of edited CAR-T cells revealed a safe integration profile of the vector, with no preferences for specific genomic regions, with highly specific editing of the HLA-I and TCR, without significant off-target sites. Finally, the production of edited CAR-T cells at a larger scale allowed the generation and selection of enough HLA-IKO/TCRKO CAR-T cells that would be compatible with clinical applications. In summary, our results demonstrate that CAR-T cells from AML patients, although functional, present phenotypic and functional features that could compromise their antitumoral efficacy, compared to CAR-T cells from healthy donors. The combination of CRISPR technologies with transposon-based delivery strategies allows the generation of HLA-IKO/TCRKO CAR-T cells, compatible with allogeneic approaches, that would represent a promising option for AML treatment.
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- 2023
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3. Cerebral metabolic pattern associated with progressive parkinsonism in non-human primates reveals early cortical hypometabolism
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Francisco Molinet-Dronda, Javier Blesa, Natalia López-González del Rey, Carlos Juri, María Collantes, Jose A Pineda-Pardo, Inés Trigo-Damas, Elena Iglesias, Ledia F. Hernández, Rafael Rodríguez-Rojas, Belén Gago, Margarita Ecay, Elena Prieto, Miguel Á. García-Cabezas, Carmen Cavada, María C. Rodríguez-Oroz, Iván Peñuelas, and José A. Obeso
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Parkinson's disease ,Positron emission tomography (PET) ,[18F]-fluoro-2-deoxy-d-glucose (18F-FDG) ,[11C]-dihydrotetrabenazine (11C-DTBZ) ,Glucose metabolism ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Dopaminergic denervation in patients with Parkinson's disease is associated with changes in brain metabolism. Cerebral in-vivo mapping of glucose metabolism has been studied in severe stable parkinsonian monkeys, but data on brain metabolic changes in early stages of dopaminergic depletion of this model is lacking. Here, we report cerebral metabolic changes associated with progressive nigrostriatal lesion in the pre-symptomatic and symptomatic stages of the progressive 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) monkey model of Parkinson's Disease. Monkeys (Macaca fascicularis) received MPTP injections biweekly to induce progressive grades of dopamine depletion. Monkeys were sorted according to motor scale assessments in control, asymptomatic, recovered, mild, and severe parkinsonian groups. Dopaminergic depletion in the striatum and cerebral metabolic patterns across groups were studied in vivo by positron emission tomography (PET) using monoaminergic ([11C]-dihydrotetrabenazine; 11C-DTBZ) and metabolic (2-[18F]-fluoro-2-deoxy-d-glucose; 18F-FDG) radiotracers. 11C-DTBZ-PET analysis showed progressive decrease of binding potential values in the striatum of monkeys throughout MPTP administration and the development of parkinsonian signs. 18F-FDG analysis in asymptomatic and recovered animals showed significant hypometabolism in temporal and parietal areas of the cerebral cortex in association with moderate dopaminergic nigrostriatal depletion. Cortical hypometabolism extended to involve a larger area in mild parkinsonian monkeys, which also exhibited hypermetabolism in the globus pallidum pars interna and cerebellum. In severe parkinsonian monkeys, cortical hypometabolism extended further to lateral-frontal cortices and hypermetabolism also ensued in the thalamus and cerebellum. Unbiased histological quantification of neurons in Brodmann's area 7 in the parietal cortex did not reveal neuron loss in parkinsonian monkeys versus controls. Early dopaminergic nigrostriatal depletion is associated with cortical, mainly temporo-parietal hypometabolism unrelated to neuron loss. These findings, together with recent evidence from Parkinson's Disease patients, suggest that early cortical hypometabolism may be associated and driven by subcortical changes that need to be evaluated appropriately. Altogether, these findings could be relevant when potential disease modifying therapies become available.
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- 2022
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4. Generation of four Isl1 reporter iPSC lines from cardiac and tail-tip fibroblasts derived from Ai6IslCre mouse
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Javier Linares, Leyre López-Muneta, Estibaliz Arellano-Viera, Purificación Ripalda-Cemboráin, Elena Iglesias, Gloria Abizanda, Xabier L. Aranguren, Felipe Prósper, and Xonia Carvajal-Vergara
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Biology (General) ,QH301-705.5 - Abstract
Islet-1 (Isl1) is a transcription factor essential for life expressed in specific cells with different developmental origins. We have generated iPSC lines from fibroblasts of the transgenic Ai6 x Isl1-Cre (Ai6IslCre) mouse. Here we describe the complete characterization of four iPSC lines: ATCi-Ai6IslCre10, ATCi-Ai6IslCre35, ATCi-Ai6IslCre74 and ATCi-Ai6IslCre80.
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- 2018
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5. Local Preirradiation of Infarcted Cardiac Tissue Substantially Enhances Cell Engraftment
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Gloria Abizanda, Leyre López-Muneta, Javier Linares, Luis I. Ramos, Arantxa Baraibar-Churio, Miriam Bobadilla, Elena Iglesias, Giulia Coppiello, Purificación Ripalda-Cemboráin, Xabier L. Aranguren, Felipe Prósper, Ana Pérez-Ruiz, and Xonia Carvajal-Vergara
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myocardial infarction ,cell therapy ,local irradiation ,cell engraftment ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
The success of cell therapy for the treatment of myocardial infarction depends on finding novel approaches that can substantially implement the engraftment of the transplanted cells. In order to enhance cell engraftment, most studies have focused on the pretreatment of transplantable cells. Here we have considered an alternative approach that involves the preconditioning of infarcted heart tissue to reduce endogenous cell activity and thus provide an advantage to our exogenous cells. This treatment is routinely used in other tissues such as bone marrow and skeletal muscle to improve cell engraftment, but it has never been taken in cardiac tissue. To avoid long-term cardiotoxicity induced by full heart irradiation we developed a rat model of a catheter-based heart irradiation system to locally impact a delimited region of the infarcted cardiac tissue. As proof of concept, we transferred ZsGreen+ iPSCs in the infarcted heart, due to their ease of use and detection. We found a very significant increase in cell engraftment in preirradiated rats. In this study, we demonstrate for the first time that preconditioning the infarcted cardiac tissue with local irradiation can substantially enhance cell engraftment.
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- 2021
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6. Generation of a Sprague-Dawley-GFP rat iPS cell line
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Giulia Coppiello, Gloria Abizanda, Natalia Aguado, Elena Iglesias, Olalla Iglesias-Garcia, Antonio Lo Nigro, Felipe Prosper, and Xabier L. Aranguren
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Biology (General) ,QH301-705.5 - Abstract
We generated a rat iPSC line called ATCi-rSD95 from transgenic Sprague-Dawley GFP fetal fibroblasts. Established ATCi-rSD95 cells present a normal karyotype, silencing of the transgenes and express pluripotency-associated markers. Additionally, ATCi-rSD95 cells are able to form teratoma with differentiated cells derived from the three germ-layers that maintain the GFP expression.
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- 2017
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7. Isolation and characterization of Sprague-Dawley and Wistar Kyoto GFP rat embryonic stem cells
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Giulia Coppiello, Gloria Abizanda, Natalia Aguado, Elena Iglesias, Olalla Iglesias-Garcia, Antonio Lo Nigro, Felipe Prosper, and Xabier L. Aranguren
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Biology (General) ,QH301-705.5 - Abstract
We generated two rat embryonic stem cell (ESC) lines: ATCe-SD7.8 from Sprague-Dawley strain and ATCe-WK1 from Wistar Kyoto strain. Cells were marked with enhanced green fluorescent protein (eGFP) by transduction with a lentiviral vector. Cells present a normal karyotype and express pluripotency-associated markers. Pluripotency was tested in vivo with the teratoma formation assay. Cells maintain eGFP expression upon differentiation to the three-germ layers. These cells can be a useful tool for cell therapy studies and chimera generation as they can be easily tracked by eGFP expression.
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- 2017
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8. Generation of Macaca fascicularis iPS cell line ATCi-MF1 from adult skin fibroblasts using non-integrative Sendai viruses
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Giulia Coppiello, Gloria Abizanda, Natalia Aguado, Elena Iglesias, Estibaliz Arellano-Viera, Juan R Rodriguez-Madoz, Xonia Carvajal-Vergara, Felipe Prosper, and Xabier L. Aranguren
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Biology (General) ,QH301-705.5 - Abstract
We generated ATCi-MF1 induced pluripotent stem (iPS) cell line from Macaca fascicularis adult skin fibroblasts using non-integrative Sendai viruses carrying OCT3/4, KLF4, SOX2 and c-MYC. Once established, ATCi-MF1 cells present a normal karyotype, are Sendai virus-free and express pluripotency associated markers. Microsatellite markers analysis confirmed the origin of the iPS cells from the parental fibroblasts. Pluripotency was tested with the in vivo teratoma formation assay. ATCi-MF1 cell line may be a useful primate iPS cell model to test different experimental conditions where the use of human cells can imply ethical issues, as microinjection of pluripotent stem cells in pre-implantational embryos.
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- 2017
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9. Generation of iPSC from cardiac and tail-tip fibroblasts derived from a second heart field reporter mouse
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Javier Linares, Estibaliz Arellano-Viera, Olalla Iglesias-García, Carmen Ferreira, Elena Iglesias, Gloria Abizanda, Felipe Prósper, and Xonia Carvajal-Vergara
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Biology (General) ,QH301-705.5 - Abstract
Mef2c Anterior Heart Field (AHF) enhancer is activated during embryonic heart development and it is expressed in multipotent cardiovascular progenitors (CVP) giving rise to endothelial and myocardial components of the outflow tract, right ventricle and ventricular septum. Here we have generated iPSC from transgenic Mef2c-AHF-Cre x Ai6(RCLZsGreen) mice. These iPSC will provide a novel tool to investigate the AHF-CVP and their cell progeny.
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- 2016
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10. Monoaminergic PET imaging and histopathological correlation in unilateral and bilateral 6-hydroxydopamine lesioned rat models of Parkinson's disease: A longitudinal in-vivo study
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Francisco Molinet-Dronda, Belén Gago, Ana Quiroga-Varela, Carlos Juri, María Collantes, Mercedes Delgado, Elena Prieto, Margarita Ecay, Elena Iglesias, Concepció Marín, Iván Peñuelas, and José A. Obeso
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Parkinson's disease ,6-Hydroxydopamine ,Positron emission tomography ,[11C]-dihydrotetrabenazine (11C-DTBZ) ,Nigro-striatal depletion ,Dopaminergic marker ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Carbon-11 labeled dihydrotetrabenazine (11C-DTBZ) binds to the vesicular monoamine transporter 2 and has been used to assess nigro-striatal integrity in animal models and patients with Parkinson's disease. Here, we applied 11C-DTBZ positron emission tomography (PET) to obtain longitudinally in-vivo assessment of striatal dopaminergic loss in the classic unilateral and in a novel bilateral 6-hydroxydopamine (6-OHDA) lesion rat model. Forty-four Sprague–Dawley rats were divided into 3 sub-groups: 1. 6-OHDA-induced unilateral lesion in the medial forebrain bundle, 2. bilateral lesion by injection of 6-OHDA in the third ventricle, and 3. vehicle injection in either site. 11C-DTBZ PET studies were investigated in the same animals successively at baseline, 1, 3 and 6 weeks after lesion using an anatomically standardized volumes-of-interest approach. Additionally, 12 rats had PET and Magnetic Resonance Imaging to construct a new 11C-DTBZ PET template. Behavior was characterized by rotational, catalepsy and limb-use asymmetry tests and dopaminergic striatal denervation was validated post-mortem by immunostaining of the dopamine transporter (DAT). 11C-DTBZ PET showed a significant decrease of striatal binding (SB) values one week after the unilateral lesion. At this point, there was a 60% reduction in SB in the affected hemisphere compared with baseline values in 6-OHDA unilaterally lesioned animals. A 46% symmetric reduction over baseline SB values was found in bilaterally lesioned rats at the first week after lesion. SB values remained constant in unilaterally lesioned rats whereas animals with bilateral lesions showed a modest (22%) increase in binding values at the 3rd and 6th weeks post-lesion. The degree of striatal dopaminergic denervation was corroborated histologically by DAT immunostaining. Statistical analysis revealed a high correlation between 11C-DTBZ PET SB and striatal DAT immunostaining values (r = 0.95, p
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- 2015
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11. Liposarcoma intraabdominal gigante
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Elena Iglesias-Jorquera, Francisco-Javier Álvarez-Higueras, Paula-Tomás Pujante, and Carmen Garre-Sánchez
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Diseases of the digestive system. Gastroenterology ,RC799-869 - Published
- 2018
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12. Anisotropic Cryostructured Collagen Scaffolds for Efficient Delivery of RhBMP–2 and Enhanced Bone Regeneration
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Kai Stuckensen, José M. Lamo-Espinosa, Emma Muiños-López, Purificación Ripalda-Cemboráin, Tania López-Martínez, Elena Iglesias, Gloria Abizanda, Ion Andreu, María Flandes-Iparraguirre, Juan Pons-Villanueva, Reyes Elizalde, Joachim Nickel, Andrea Ewald, Uwe Gbureck, Felipe Prósper, Jürgen Groll, and Froilán Granero-Moltó
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rhBMP–2 ,collagen sponge ,cryostructured scaffolds ,bone critical size defect ,Technology ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Microscopy ,QH201-278.5 ,Descriptive and experimental mechanics ,QC120-168.85 - Abstract
In the treatment of bone non-unions, an alternative to bone autografts is the use of bone morphogenetic proteins (BMPs), e.g., BMP−2, BMP−7, with powerful osteoinductive and osteogenic properties. In clinical settings, these osteogenic factors are applied using absorbable collagen sponges for local controlled delivery. Major side effects of this strategy are derived from the supraphysiological doses of BMPs needed, which may induce ectopic bone formation, chronic inflammation, and excessive bone resorption. In order to increase the efficiency of the delivered BMPs, we designed cryostructured collagen scaffolds functionalized with hydroxyapatite, mimicking the structure of cortical bone (aligned porosity, anisotropic) or trabecular bone (random distributed porosity, isotropic). We hypothesize that an anisotropic structure would enhance the osteoconductive properties of the scaffolds by increasing the regenerative performance of the provided rhBMP−2. In vitro, both scaffolds presented similar mechanical properties, rhBMP−2 retention and delivery capacity, as well as scaffold degradation time. In vivo, anisotropic scaffolds demonstrated better bone regeneration capabilities in a rat femoral critical-size defect model by increasing the defect bridging. In conclusion, anisotropic cryostructured collagen scaffolds improve bone regeneration by increasing the efficiency of rhBMP−2 mediated bone healing.
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- 2019
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13. Enterococcus gallinarum and Chilaiditi syndrome in peritoneal dialysis
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Cristina Pérez Melón, Beatriz Ferreiro Álvarez, Elena Iglesias Lamas, María Jesús Camba Caride, María Paz Borrajo Prol, and Enrique Novoa Fernández
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Diseases of the genitourinary system. Urology ,RC870-923 - Published
- 2017
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14. Enterococcus gallinarum y síndrome de Chilaiditi en diálisis peritoneal
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Cristina Pérez Melón, Beatriz Ferreiro Álvarez, Elena Iglesias Lamas, María Jesús Camba Caride, María Paz Borrajo Prol, and Enrique Novoa Fernández
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Diseases of the genitourinary system. Urology ,RC870-923 - Published
- 2017
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15. Progression of dopaminergic depletion in a model of MPTP-induced Parkinsonism in non-human primates. An 18F-DOPA and 11C-DTBZ PET study
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Javier Blesa, Carlos Juri, María Collantes, Iván Peñuelas, Elena Prieto, Elena Iglesias, Josep Martí-Climent, Javier Arbizu, José L. Zubieta, Mari Cruz Rodríguez-Oroz, David García-García, José A. Richter, Carmen Cavada, and José A. Obeso
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PET ,Parkinson's disease ,MPTP-induced parkinsonism ,Non-human primates ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Dopaminergic depletion in the nigrostriatal system is the neurochemical hallmark of Parkinson's disease (PD). Although numerous efforts have been made to determine the evolution of dopaminergic depletion in PD, “in vivo” data concerning the stages of this process are still scarce. We evaluated 6-[18F]-fluoro-l-DOPA (18F-DOPA) and 11C-(+)-α-dihydrotetrabenazine (11C-DTBZ) using PET in a model of chronically MPTP-induced parkinsonism in non-human primates. Methods: Sixty-seven cynomolgus monkeys (Macaca fascicularis) were included in the study. Progressive parkinsonism was induced by repeated administration of small doses of MPTP (iv) over several months. Animals were classified as controls, asymptomatic, recovered (having exhibited parkinsonian features transiently) and stable parkinsonian, according to their motor status. Analysis of striatal dopaminergic activity was conducted by regions of interest (ROI) and statistical parametric mapping (SPM) over normalized parametric images. Results: A progressive loss of striatal uptake was evident among groups for both radiotracers, which correlated significantly with the clinical motor status. Changes occurred earlier, i.e. in the less affected stages, with 11C-DTBZ. Similar results were achieved by ROI and SPM analysis. Uptake was similar with both radiotracers for the asymptomatic and recovered groups. Conclusions: Serial assessment with 18F-DOPA and 11C-DTBZ PETs provides an effective approach to evaluate evolution of dopaminergic depletion in monkeys with MPTP-induced parkinsonism. This approach could be useful to perform studies aiming to test the effect of early therapeutic intervention and putative neuroprotective treatments.
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- 2010
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16. Daptomicina en diálisis peritoneal, intraperitoneal o intravenosa
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Cristina Pérez Melón, Maria Borrajo Prol, Elena Iglesias, Beatriz Ferreiro, and Maria Camba Caride
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Diseases of the genitourinary system. Urology ,RC870-923 - Published
- 2016
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17. Daptomycin in peritoneal dialysis, intraperitoneal or intravenous
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Cristina Pérez Melón, Maria Borrajo Prol, Elena Iglesias, Beatriz Ferreiro, and Maria Camba Caride
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Diseases of the genitourinary system. Urology ,RC870-923 - Published
- 2016
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18. Overt obscure gastrointestinal bleeding secondary to small bowel gastrointestinal stromal tumor diagnosed by videocapsule endoscopy
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Gabriel Carrilero-Zaragoza, Guillermo Carbonell-López-del-Castillo, Gema Ruiz-García, Juan Egea-Valenzuela, Paula Tomas-Pujante, Elena Iglesias-Jorquera, Josefa Parra-García, Antonio Sánchez-Torres, Elena Navarro-Noguera, Esther Estrella-Díaz, and Luis Fernando Carballo-Álvarez
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Diseases of the digestive system. Gastroenterology ,RC799-869 - Published
- 2015
19. Recuperación del paisaje con fines de ocio. Parque fluvial del Jarama. Comunidad de Madrid. España
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Alejandro Pinedo, Elena Iglesias, and Ana Pilar Espluga
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Architecture ,NA1-9428 ,Building construction ,TH1-9745 - Abstract
El plan de recuperación de márgenes y riberas que se describe en el presente artículo, trata de salir al paso y aportar soluciones a los cada vez más frecuentes problemas de restauración y aprovechamiento de terrenos degradados que se plantean en los ámbitos urbanos y periurbano. El plan se localiza geográficamente en un área de la Comunidad de Madrid que posee un alto nivel de degradación y al mismo tiempo un gran potencial recreativo, se trata de el Parque Fluvial del Jarama, situado en el tramo del río Jarama comprendido entre la desembocadura del arroyo de la Vega y la del Manzanares; el Parque Fluvial del Jarama constituye la actuación más ambiciosa dentro del Programa de Recuperación de Márgenes del Plan Integral del Agua en Madrid (PIAM) y en él se contemplan una serie de actuaciones que se pueden agrupar en los bloques siguientes: — Restauración de la explanación del ferrocarril. — Restauración y adecuación del cauce y márgenes. — Restauración de la Laguna del Campillo.
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- 1990
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20. Un caso de estudio de impacto ambiental: el embalse de Vallfornés. Cataluña-España
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Alejandro Pinedo and Elena Iglesias
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Architecture ,NA1-9428 ,Building construction ,TH1-9745 - Abstract
Los estudios de impacto ambiental surgen de la necesidad de proyectar las actividades de la forma más adecuada posible, que facilite su integración en el medio natural en que han de inscribirse para que su calidad no se vea afectada. El caso del embalse de Vallfornés, cuya finalidad principal es el abastecimiento de agua para regadío, presenta además la peculiaridad de afectar a terrenos Incluidos dentro del Parque Natural de Montseny, lo que obliga no sólo a un tratamiento cuidadoso de la obra, sino también a la resolución de conflictos inevitables con la normativa existente. En el presente artículo se pasa revista a los principales aspectos del estudio de impacto ambiental de dicho embalse: análisis de las implicaciones derivadas de su ubicación en terrenos del Parque Natural del Montseny, caracterización del medio afectado, identificación de las acciones productoras de impacto, caracterización y valoración de los mismos y recomendaciones respecto a la corrección de los impactos potenciales.
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- 1990
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21. In vivo cholinergic basal forebrain degeneration and cognition in Parkinson's disease: Imaging results from the COPPADIS study
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Grothe, Michel J., Labrador-Espinosa, Miguel A., Jesús, Silvia, Macías-García, Daniel, Adarmes-Gómez, Astrid, Carrillo, Fátima, Camacho, Elena Iglesias, Franco-Rosado, Pablo, Lora, Florinda Roldán, Martín-Rodríguez, Juan Francisco, Barberá, Miquel Aguilar, Pastor, Pau, Arroyo, Sonia Escalante, Vila, Berta Solano, Foraster, Anna Cots, Martínez, Javier Ruiz, Padilla, Francisco Carrillo, Morlans, Mercedes Pueyo, Aramburu, Isabel González, Ceberio, Jon Infante, Vara, Jorge Hernández, de Fábregues-Boixar, Oriol, de Deus Fonticoba, Teresa, Pascual-Sedano, Berta, Kulisevsky, Jaime, Martínez-Martín, Pablo, Santos-García, Diego, and Mir, Pablo
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- 2021
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22. 1762 - INFECCIÓN POR CLOSTRIDIUM DIFFICILE: CARACTERÍSTICAS CLÍNICAS, FACTORES DE RIESGO
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Santiago, Jose Ángel Pestaña, primary, Rincón, Erika Esperanza Lozano, additional, León, Juan Francisco Bayona, additional, Saleh, Wafa Elgeadi, additional, Morante, Daniel Alejandro García, additional, Gil, Elena Iglesias, additional, Colino, Laura larrin, additional, and Osorio., Luis Cabeza, additional
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- 2023
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23. 2167 - EVALUACIÓN DE PRUEBAS DE DETECCIÓN Y CARACTERÍSTICAS DEMOGRÁFICAS DE LA FARINGITIS POR S. PYOGENES EN EL PRIMER TRIMESTRE DEL AÑO 2022 Y 2023, EN LA UNIDAD DE URGENCIAS DEL HOSPITAL UNIVERSITARIO DEL HENARES
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Morante, Daniel Alejandro García, primary, Rincon, Erika Esperanza Lozano, additional, Leon, Juan Francisco Bayona, additional, Santiago, Jose Ángel Pestaña, additional, Saleh, Wafa Elgeadi, additional, Gil, Elena Iglesias, additional, Muñoz, Carlota Madrigal, additional, and Colino, Laura Larrin, additional
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- 2023
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24. Factors associated with the presence of abnormal levels of fecal calprotectin in patients with negative panenteric studies
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Valenzuela, Juan Egea, Jorquera, Elena Iglesias, Higueras, Francisco Javier Alvarez, Tornero, Maria Munoz, Lopez, Ana Pereniguez, Diez, Esther Estrella, and de las Parras, Fernando Alberca
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- 2020
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25. Spontaneous retroperitoneal hematoma in a patient under anticoagulant agents presenting as upper gastrointestinal bleeding
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Gabriel Carrilero-Zaragoza, Juan Egea-Valenzuela, María Moya-Arnao, María Muñoz-Tornero, Roxanna Jijón-Crespín, Paula Tomás-Pujante, Elena Iglesias-Jorquera, Josefa Parra-García, Eduardo Sánchez-Velasco, Ana Pereñíguez-López, Manuel Miras-López, Matilde Fuster-Quiñonero, and Fernando Carballo-Álvarez
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JAK-2 ,Duodenal ulcer ,Retroperitoneal hematoma ,Gastrointestinal bleeding ,Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
We present the case of a 44-year-old woman with past history of repeated miscarriage and Budd-Chiari syndrome secondary to primary myelofibrosis. Because of this she was under treatment with oral anticoagulant agents. The patient was admitted in hospital as she presented with gastrointestinal bleeding (melena), asthenia and progressive anemia. In an initial upper endoscopy an extrinsic duodenal compression associated with an ulcer on the posterior face of the first portion of duodenum and upper duodenal knee was observed. In the following days a huge spontaneous retroperitoneal hematoma due to anticoagulation was diagnosed by computed tomography. This was treated with a percutaneous drainage and withdrawal of the antithrombotic drugs. The evolution of the patient was initially satisfactory but she suffered subclavian and jugular vein thrombosis, and reintroduction of anticoagulant agents at the lowest therapeutic doses was required.
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26. Encefalopatía hepática secundaria a shunt esplenorrenal que se manifiesta tras largo tiempo del trasplante hepático
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Paula Tomás-Pujante, Andrés-Francisco Jiménez-Sánchez, Elena Iglesias-Jorquera, and José-Antonio Pons-Miñano
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Diseases of the digestive system. Gastroenterology ,RC799-869 - Full Text
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27. Expanded hemodialysis: what’s up, Doc?
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Ana García-Prieto, José Carlos de la Flor, Elisabet Coll, Elena Iglesias, Javier Reque, and Francisco Valga
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Transplantation ,Nephrology - Abstract
In recent years there has been an increasing interest in expanded hemodialysis (HDx), an emerging renal replacement therapy based on the use of medium cut-off membranes (MCO). Thanks to the internal architecture of these types of membranes, with a higher pore size and smaller fiber inner diameter to favor internal filtration rate, they can increase the removal of larger middle molecules in conventional hemodialysis. Secondarily, several reports suggest that this therapy potentially improve the outcomes for end-stage renal disease patients. However, HDx has not been defined yet and the characteristics of MCO membranes are not well stablished. The aim of this narrative review is to define HDx and summarize the dialyzers that have been used so far to perform this therapy, collect the evidence available on its efficacy and clinical outcomes compared with other hemodialysis techniques and settle the bases for its optimal prescription.
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- 2023
28. In vivo generation of heart and vascular system by blastocyst complementation
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Giulia Coppiello, Paula Barlabé, Marta Moya-Jódar, Gloria Abizanda, Carolina Barreda, Elena Iglesias, Javier Linares, Estibaliz Arellano-Viera, Adrian Ruiz-Villalba, Eduardo Larequi, Xonia Carvajal-Vergara, Beatriz Pelacho, Felipe Prósper, and Xabier L. Aranguren
- Abstract
SUMMARYThe generation of organs from stem cells by blastocyst complementation is a promising approach to cover the clinical need for transplants. In order to generate rejection-free organs, complementation of both parenchymal and vascular cells must be achieved, as endothelial cells play a key role in graft rejection. Here we used a lineage-specific cell ablation system to produce mouse embryos unable to form both the cardiac and vascular systems. By mouse intraspecies blastocyst complementation we rescued heart and vascular development separately and in combination, obtaining complemented hearts with cardiomyocytes and endothelial cells of exogenous origin. Complemented chimeras were viable and reached adult stage, showing normal cardiac function and no signs of histopathological defects in the heart. Furthermore, we implemented the cell ablation system for rat-to-mouse blastocyst complementation, obtaining xenogeneic hearts whose cardiomyocytes were completely of rat origin. These results represent an advance in the experimentation towards the in vivo generation of transplantable organs.
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- 2022
29. Modifiers of Health-Related Quality of Life by Biological, Psychological and Social Factors
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Elena Iglesias De Sena, Maria-Fernanda Lorenzo Gómez, José Antonio Mirón Canelo, and Luz Celia Fernández Martín
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Gerontology ,Health related quality of life ,03 medical and health sciences ,0302 clinical medicine ,030212 general & internal medicine ,030204 cardiovascular system & hematology ,Psychology - Abstract
Healthcare workers, clinicians and/or researchers require information on the consequences of illness on their patients, as well as on the effects associated with treatments, when making decisions on recommended treatments and for follow-up evaluations of the same. Identifying health indicators which provide necessary and appropriate data for the evaluation of clinical outcomes in terms of Health-Related Quality of Life (HRQoL), as established by the WHO Biopsychosocial Model, and which provide appropriate and pertinent information on physical, mental and social factors in patients, can improve decision-making in relation to a comprehensive and global perspective of clinical outcomes of the various treatments and procedures given to patients. This chapter aims to provide an overview of the various tools for assessing Health-Related Quality of Life, as a growing number of clinicians, researchers and patient groups wish for comprehensive and not merely biological measures of health. This may be explained by the growing number of self-administered or interview questionnaires which have the aim of measuring changes in health as well as the consequences of the various treatments used mainly on chronicity and chronic health conditions. During recent decades, numerous tools have been developed and applied to the measurement of the effects of Health-Related Quality of Life in patients based on biological or physical aspects, psychological or mental aspects, and social aspects. This chapter will review the most frequently-used tools for the measurement of Health-Related Quality of Life, and recommendations are made for their use in medical care according to psychometric characteristics and quality criteria, as a guide for use in the field of healthcare, in public health, or in outcomes research.
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- 2022
30. Effect of Roux-en-Y Gastric Bypass on Lipoprotein Metabolism and Markers of HDL Functionality in Morbid Obese Patients
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Patricia Sorroche, Susana Gutt, Eliana Elizabeth Botta, Laura Boero, Gloria Edith Cerrone, Mercedes Gutierrez, Axel Beskow, Fernando Brites, Walter Francisco Tetzlaff, Andrea Elena Iglesias Molli, Florencia Ferraro, Andrea Millán, Gustavo Daniel Frechtel, Maria V. Lorenzon Gonzalez, María Soledad Saez, Maximiliano Martin, and Ezequiel Lozano Chiappe
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medicine.medical_specialty ,Lipoproteins ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,Gastric Bypass ,030209 endocrinology & metabolism ,Carbohydrate metabolism ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Insulin resistance ,Internal medicine ,Cholesterylester transfer protein ,medicine ,Humans ,Nutrition and Dietetics ,biology ,medicine.diagnostic_test ,Triglyceride ,business.industry ,Insulin ,Cholesterol, HDL ,Paraoxonase ,Cholesterol, LDL ,medicine.disease ,Obesity ,Cholesterol Ester Transfer Proteins ,Obesity, Morbid ,Endocrinology ,chemistry ,biology.protein ,lipids (amino acids, peptides, and proteins) ,030211 gastroenterology & hepatology ,Surgery ,Lipid profile ,business - Abstract
Morbid obesity represents the most severe form of obesity and surgical intervention would be its only successful treatment. Bariatric surgery could generate modifications in carbohydrate metabolism and in lipid profile plus lipoprotein-associated proteins and enzymes, such as lipoprotein-associated phoslipase A2 (Lp-PLA2), cholesteryl ester transfer protein (CETP), and paraoxonase (PON) 1. The aim of the present study was to analyze changes in inflammation markers, carbohydrate metabolism, and lipid parameters in patients who underwent bariatric surgery. Thirty-seven patients with morbid obesity were recruited. Evaluations were performed before (T0) and 1 (T1) and 6 (T2) months after surgery. Glucose, insulin, high-sensitivity C-reactive protein (hsCRP), triglycerides, total cholesterol, high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol, apolipoproteins (apo) A-I, and B plus Interleukin 1β and 6 levels in addition to CETP, Lp-PLA2, and PON 1 activities were determined. Body mass index decreased at T1 and T2 (p
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- 2020
31. Cerebral metabolic pattern associated with progressive parkinsonism in non-human primates reveals early cortical hypometabolism
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Francisco Molinet-Dronda, Javier Blesa, Natalia López-González del Rey, Carlos Juri, María Collantes, Jose A Pineda-Pardo, Inés Trigo-Damas, Elena Iglesias, Ledia F. Hernández, Rafael Rodríguez-Rojas, Belén Gago, Margarita Ecay, Elena Prieto, Miguel Á. García-Cabezas, Carmen Cavada, María C. Rodríguez-Oroz, Iván Peñuelas, and José A. Obeso
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Cerebral Cortex ,Primates ,Glucose metabolism ,Parkinson's disease ,Dopamine ,[(18)F]-fluoro-2-deoxy-d-glucose ((18)F-FDG) ,Corpus Striatum ,Neurology ,Parkinsonian Disorders ,1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine ,Positron emission tomography (PET) ,Positron-Emission Tomography ,Animals ,Humans ,[(11)C]-dihydrotetrabenazine ((11)C-DTBZ) - Abstract
Dopaminergic denervation in patients with Parkinson's disease is associated with changes in brain metabolism. Cerebral in-vivo mapping of glucose metabolism has been studied in severe stable parkinsonian monkeys, but data on brain metabolic changes in early stages of dopaminergic depletion of this model is lacking. Here, we report cerebral metabolic changes associated with progressive nigrostriatal lesion in the pre-symptomatic and symptomatic stages of the progressive 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) monkey model of Parkinson's Disease. Monkeys (Macaca fascicularis) received MPTP injections biweekly to induce progressive grades of dopamine depletion. Monkeys were sorted according to motor scale assessments in control, asymptomatic, recovered, mild, and severe parkinsonian groups. Dopaminergic depletion in the striatum and cerebral metabolic patterns across groups were studied in vivo by positron emission tomography (PET) using monoaminergic ([11C]-dihydrotetrabenazine; 11C-DTBZ) and metabolic (2-[18F]-fluoro-2-deoxy-d-glucose; 18F-FDG) radiotracers. 11C-DTBZ-PET analysis showed progressive decrease of binding potential values in the striatum of monkeys throughout MPTP administration and the development of parkinsonian signs. 18F-FDG analysis in asymptomatic and recovered animals showed significant hypometabolism in temporal and parietal areas of the cerebral cortex in association with moderate dopaminergic nigrostriatal depletion. Cortical hypometabolism extended to involve a larger area in mild parkinsonian monkeys, which also exhibited hypermetabolism in the globus pallidum pars interna and cerebellum. In severe parkinsonian monkeys, cortical hypometabolism extended further to lateral-frontal cortices and hypermetabolism also ensued in the thalamus and cerebellum. Unbiased histological quantification of neurons in Brodmann's area 7 in the parietal cortex did not reveal neuron loss in parkinsonian monkeys versus controls. Early dopaminergic nigrostriatal depletion is associated with cortical, mainly temporo-parietal hypometabolism unrelated to neuron loss. These findings, together with recent evidence from Parkinson's Disease patients, suggest that early cortical hypometabolism may be associated and driven by subcortical changes that need to be evaluated appropriately. Altogether, these findings could be relevant when potential disease modifying therapies become available.
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- 2021
32. Telomere Length Differently Associated to Obesity and Hyperandrogenism in Women With Polycystic Ovary Syndrome
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Mariela Edith Velazquez, Andrea L. Millan, Mailén Rojo, Giselle Adriana Abruzzese, Silvina Ema Cocucci, Andrea Elena Iglesias Molli, Gustavo Daniel Frechtel, Alicia Beatriz Motta, and Gloria Edith Cerrone
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Adult ,medicine.medical_specialty ,obesity ,Waist ,endocrine system diseases ,medicine.drug_class ,Endocrinology, Diabetes and Metabolism ,Argentina ,030209 endocrinology & metabolism ,metabolic and endocrine disorders ,Diseases of the endocrine glands. Clinical endocrinology ,Body Mass Index ,hyperandrogenism ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,Internal medicine ,hemic and lymphatic diseases ,medicine ,telomere length ,Humans ,Testosterone ,Retrospective Studies ,Original Research ,030219 obstetrics & reproductive medicine ,business.industry ,Polycystic ovary syndrome (PCOS) ,Hyperandrogenism ,Telomere Homeostasis ,nutritional and metabolic diseases ,Telomere ,Androgen ,medicine.disease ,RC648-665 ,Obesity ,Polycystic ovary ,female genital diseases and pregnancy complications ,polycystic ovary syndrome ,Case-Control Studies ,Female ,business ,Hormone - Abstract
BackgroundPolycystic Ovary Syndrome (PCOS) often present metabolic disorders and hyperandrogenism (HA), facts that may influence the telomere length (TL).AimsTo compare the absolute TL (aTL) between women with PCOS and control women, and their association with the presence of obesity and HA parameters.Materials and methodsThe PCOS group included 170 unrelated women outpatients and the control group, 64 unrelated donor women. Anthropometric, biochemical-clinical parameters and androgen profile were determined. The PCOS patients were divided accordingly to the presence of obesity and androgenic condition. The aTL was determined from peripheral blood leukocytes by Real Time quantitative PCR.ResultsWomen with PCOS exhibited a significantly longer aTL than controls after age adjustment (p=0.001). A stepwise multivariate linear regression in PCOS women, showed that WC (waist circumference) contributed negatively (b=-0.17) while testosterone levels contributed positively (b=7.24) to aTL. The non-Obese PCOS (noOB-PCOS) presented the longest aTL when compared to controls (p=0.001). Meanwhile, the aTL was significantly higher in the hyperandrogenic PCOS phenotype (HA-PCOS) than in the controls (p=0.001) and non hyperandrogenic PCOS phenotype (NHA-PCOS) (p=0.04). Interestingly, when considering obesity and HA parameters in PCOS, HA exerts the major effect over the aTL as non-obese HA exhibited the lengthiest aTL (23.9 ± 13.13 Kbp). Conversely, the obese NHA patients showed the shortest aTL (16.5 ± 10.59 Kbp).ConclusionsWhilst a shorter aTL could be related to the presence of obesity, a longer aTL would be associated with HA phenotype. These findings suggest a balance between the effect produced by the different metabolic and hormonal components, in PCOS women.
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- 2021
33. Endoscopic treatment of postoperative esophagogastric leaks with fully covered self-expanding metal stents
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David Ruiz de Angulo, Andrés Serrano Jiménez, Juan Egea Valenzuela, Fernando Alberca de Las Parras, Elena Iglesias Jorquera, Pascual Parrilla, Vicente Munitiz, Gabriel Carrilero Zaragoza, Eduardo Sánchez Velasco, and Antonio Ortega Sabater
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medicine.medical_specialty ,Leak ,Fistula ,medicine.medical_treatment ,Self Expandable Metallic Stents ,Anastomotic Leak ,03 medical and health sciences ,0302 clinical medicine ,Postoperative Complications ,medicine ,Humans ,cardiovascular diseases ,Retrospective Studies ,medicine.diagnostic_test ,business.industry ,Stomach ,Gastroenterology ,Stent ,Retrospective cohort study ,Endoscopy ,General Medicine ,equipment and supplies ,medicine.disease ,Surgery ,surgical procedures, operative ,medicine.anatomical_structure ,Treatment Outcome ,030220 oncology & carcinogenesis ,030211 gastroenterology & hepatology ,Stents ,business ,Complication ,Endoscopic treatment - Abstract
Background: the management of postoperative esophageal leaks is a huge therapeutic challenge. Thanks to the advances in endoscopy, treatment with esophageal stents has been proposed as a valid option. Aims: the main objective of the study was to evaluate the effectiveness and safety of the use of fully covered esophageal metal stents in the treatment of postoperative esophageal leaks. Methods: a retrospective observational study was performed in patients with postoperative esophageal leaks, treated with fully covered self-expandable metal stents between June 2011 and May 2018. Results: twenty-five patients were evaluated and 34 stents were placed. The closure of the leak was observed in 21 patients after removal of the stent, with an overall technical success rate of 84 %. The mean time with a stent placed for closure of the fistula was 55.7 ± 27.11 days/patient (mean of 39 ± 24.30 days/stent). The most frequent complication was a partial distal stent migration (7/34 stents), followed by five cases of complete migration into the stomach. Conclusions: endoscopic treatment with fully covered self-expandable metal stents seems to be an effective and safe first-line treatment for postoperative esophageal leaks, according to the experience in our center.
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- 2020
34. Caja de Juegos. El azar como estrategia proyectual
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Jesus Ulargui Agurruza, Sergio de Miguel Garcia, Nestor Montenegro Mateos, Javier Mosquera González, CARMEN GARCIA SANCHEZ, María Isabel Collado Baíllo, Carlos Palacios Rodríguez, Manuel Pascual García, Ignacio Senra Fernández-Miranda, Rodrigo Delso Gutiérrez, Elena Iglesias Rodríguez, Jorge Gabaldón Guzmán, and Teresa Martínez López
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Diseño ,Gamificación ,Intuición ,Educación ,Educación superior ,Arte ,Estrategia educativa ,Arquitectura ,Urbanismo - Abstract
Se propone una metodología docente alternativa para la realización de proyectos arquitectónicos, en el primer curso de esta asignatura en la Escuela Técnica Superior de Arquitectura de Madrid. Se utiliza una caja de juegos creada por el equipo docente, formada por cuatro barajas diferentes, en la que la elección fortuita de sus cartas sirve como desencadenante del proceso creativo del alumno. Se toma como referencia principal el proyecto realizado en 1975 por el músico Brian Eno y el artista Peter Schmidt, titulado “Oblique Strategies. Over One Hundred Worthwhile Dilemmas”, consistente en un conjunto de tarjetas con mensajes evocadores capaces de fomentar el pensamiento lateral en situaciones en la que el artista se ve bloqueado. A partir de provocaciones controladas, diferentes estrategias o trucos, contenidos en una serie de cartas que cada alumno debe ir descubriendo de forma a aleatoria a lo largo del curso, se plantean analogías, comparando ideas desvinculadas de lo racional, con ánimo de alejarse de los estereotipos, e indagar en la posibilidad de diferentes opciones. Se ensaya la inversión de conceptos y procesos, alterando su sentido para reconocer su contrario y experimentar el problema de manera diferente, descubriendo nuevos órdenes y modelos de entendimiento. La negación de las características obvias permite generar nuevas situaciones posibles. Maniobras todas fruto del acierto y la intensidad, las cartas se convertirán en la herramienta desde la que comenzar estas acciones. El desarrollo del juego incluye tres ejercicios con una duración de cuatro semanas cada uno de ellos, en los que deben proponer un espacio de juegos, un conjunto de espacios habitables y una estructura identificada como espacio comunitario. De cada uno de ellos realizarán los planos y las maquetas necesarias para su comprensión. Organizada en cuatro grandes grupos, Programa, Material, Acción y Sugerencia, las cartas de cada uno de estos apartados marcan el devenir de la partida. El movimiento de inicio obliga a cada alumno a coger una de cada mazo, configurando así la posición de partida de su curso. Tras la finalización del primer ejercicio, y habiendo realizado una encuesta a los alumnos sobre la pertinencia de la metodología empleada, algo más de la mitad consideran las cartas como una herramienta que facilita el desarrollo del proyecto mientras que el resto ha tenido dificultades al interpretar las instrucciones derivadas de su uso. Sin embargo, la gran mayoría de ellos, cerca del 80%, admiten que los resultados obtenidos son buenos, tanto por innovadores como por inesperados. El uso de esta nueva baraja permite afirmar que la combinación del azar y el rigor en la docencia de proyectos arquitectónicos lleva al alumno a generar soluciones alejadas de las ideas preconcebidas con las que afronta sus primeros cursos. La primera variable, como componente capaz de desbloquear y desencadenar proceso creativos, y la segunda, como confirmación y materialización de unas intuiciones desde las herramientas específicas del arquitecto. Las posibilidades de combinación, intercambio y adición de cartas a partir de las iniciales, ofrece un recorrido ilimitado en el desarrollo individual de los proyectos en el aula, derivado de la interpretación personal de los mensajes descubiertos en las cartas. Dentro del marco de la enseñanza de los primeros cursos de la asignatura de proyectos arquitectónicos, la herramienta permite acelerar el proceso creativo, concediendo al azar y a la intuición un papel principal que más tarde debe ser refrendado por la razón en el desarrollo completo de los ejercicios propuestos. No existe una única respuesta correcta al problema sino que dependiendo de la capacidad de cada alumno de modificar sus propuestas, lo que permite alcanzar una solución válida como parte de un proceso creativo evolutivo.
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- 2020
35. Relationship between the IL-1β serum concentration, mRNA levels and rs16944 genotype in the hyperglycemic normalization of T2D patients
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Mónica Paula Spalvieri, Andrea Elena Iglesias Molli, María Fernanda Bergonzi, Gustavo Daniel Frechtel, María Amelia Linari, and Gloria Edith Cerrone
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0301 basic medicine ,Normalization (statistics) ,Adult ,Blood Glucose ,Male ,medicine.medical_specialty ,Genotype ,Molecular biology ,Interleukin-1beta ,lcsh:Medicine ,INFLAMACIÓN ,Article ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,INTERLEUQUINA 1BETA ,Medical research ,purl.org/becyt/ford/3.2 [https] ,Genetics ,Medicine ,Humans ,Hypoglycemic Agents ,Genetic Predisposition to Disease ,Prospective Studies ,lcsh:Science ,Alleles ,Aged ,Gynecology ,Multidisciplinary ,Molecular medicine ,business.industry ,lcsh:R ,Serum concentration ,Middle Aged ,GENETICA ,030104 developmental biology ,Mrna level ,Diabetes Mellitus, Type 2 ,Hyperglycemia ,DIABETES TIPO2 ,Leukocytes, Mononuclear ,lcsh:Q ,purl.org/becyt/ford/3 [https] ,Female ,business ,030217 neurology & neurosurgery - Abstract
To evaluate Interleukin 1-beta (IL-1β) serum and mononuclear leucocyte mRNA levels, also rs16944 (−511C/T) genotype, in relation to hyperglycemic normalization in Type 2 diabetes (T2D) patients, we recruited 30 individuals recently T2D diagnosed with hyperglycemia studied at basal time and after 6 and 12 months of the normalization treatment. At basal time, the T polymorphic allele of the rs16944 was associated with lower IL-1β mRNA expression (p = 0.006); and higher glucose level was positive correlated to IL-1β protein levels (p = 0.015). After treatment, the individuals showed a significant decrease in glucose level (p = 0.003), but they did not express significant changes in the IL-1β serum levels. Surprisingly, we observed that the greater decreases in glucose level were associated to increased IL-1β serum levels (p = 0.040). This is the first follow-up study evaluating IL-1β mRNA expression and serum levels in hyperglycemic T2D individuals and after glycemic normalization treatment. The current results contribute to the knowledge of the relationship between inflammation and glucose metabolism in T2D. Fil: Iglesias Molli, Andrea Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina Fil: Bergonzi, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina Fil: Spalvieri, Mónica Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina Fil: Linari, María Amelia. Union Obrera Metalurgica.; Argentina Fil: Frechtel, Gustavo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Genética y Biología Molecular; Argentina Fil: Cerrone, Gloria Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Genética y Biología Molecular; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
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- 2020
36. Factors associated with the presence of abnormal levels of fecal calprotectin in patients with negative panenteric studies
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Esther Estrella Diez, Fernando Alberca de Las Parras, María Muñoz Tornero, Elena Iglesias Jorquera, Francisco Javier Álvarez-Higueras, Ana Pereñíguez López, and Juan Egea Valenzuela
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Gynecology ,Adult ,medicine.medical_specialty ,business.industry ,Gastroenterology ,General Medicine ,Middle Aged ,Capsule Endoscopy ,Feces ,Young Adult ,Medicine ,Humans ,In patient ,Female ,Prospective Studies ,Calprotectin ,business ,Leukocyte L1 Antigen Complex ,Biomarkers - Abstract
Introduccion: la calprotectina fecal es un parametro util a la hora de decidir si un paciente se puede beneficiar de la realizacion de estudios endoscopicos. No obstante, cierto numero de individuos sintomaticos y con cifras elevadas de este marcador no tendran lesiones endoscopicas. El objetivo de este estudio es determinar que factores se asocian a cifras alteradas de calprotectina fecal en pacientes con estudios endoscopicos de intestino delgado y colon normales. Metodos: estudio prospectivo y observacional de pacientes con sintomas digestivos. Se ha analizado la asociacion de diferentes variables con cifras alteradas de calprotectina y estudios endoscopicos (capsula de intestino delgado y colonoscopia) negativos. Resultados: se incluyen 143 pacientes (98 mujeres – 68,5 %), con una edad media 40,06 ± 16,42 (15-82) anos. El consumo de tabaco y la toma de antiinflamatorios no esteroideos se asocio a la presencia de cifras alteradas de calprotectina y estudios endoscopicos negativos (p: 0,029 y p: 0,006). Los valores medios de calprotectina fecal fueron significativamente mayores entre los fumadores, consumidores de antiinflamatorios y pacientes con test positivo de sobrecrecimiento bacteriano de intestino delgado. El tabaquismo (OR: 3,505; p: 0,028), la toma de antiinflamatorios (OR: 3,473; p: 0,021) y el sobrecrecimiento bacteriano (OR: 3,172; p: 0,013) son factores de riesgo independientes para presentar una calprotectina alterada sin lesiones endoscopicas. No se obtuvo asociacion con ninguna otra variable. Conclusiones: el consumo de tabaco y de antiinflamatorios no esteroideos tiene una fuerte asociacion con la presencia de valores elevados de calprotectina en ausencia de lesiones endoscopicas. Se ha observado tambien asociacion con el sobrecrecimiento bacteriano de intestino delgado.
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- 2020
37. Bioimpedance and Brain Natriuretic Peptide in Peritoneal Dialysis Patients
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Crepaldi, Carlo, primary, Lamas, Elena Iglesias, additional, Martino, Francesca Katiana, additional, Rodighiero, Maria Pia, additional, Scalzotto, Elisa, additional, Wojewodzka-Zelezniakowicz, Marzena, additional, Rosner, Mitchell H., additional, and Ronco, Claudio, additional
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- 2012
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38. Análisis histórico de la experiencia en cápsula endoscópica de intestino delgado en un hospital terciario español
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Fernando Alberca de Las Parras, Juan Egea Valenzuela, Gabriel Carrilero Zaragoza, Fernando Carballo Álvarez, Paula Tomás Pujante, and Elena Iglesias Jorquera
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03 medical and health sciences ,0302 clinical medicine ,Hepatology ,business.industry ,030220 oncology & carcinogenesis ,Gastroenterology ,Medicine ,030211 gastroenterology & hepatology ,business ,Humanities - Abstract
Resumen Introduccion el uso de la enteroscopia con videocapsula fue aprobado por la FDA en 2001. La hemorragia digestiva y la enfermedad inflamatoria intestinal son sus principales indicaciones. En nuestro centro se realiza desde 2004. Material y metodos Hemos recogido de forma retrospectiva los pacientes tratados mediante capsula de intestino delgado desde octubre de 2004 hasta abril de 2015. Las indicaciones se han dividido en grupos: hemorragia digestiva de origen oscuro, tanto oculta como manifiesta; enfermedad inflamatoria intestinal; otras indicaciones. Los hallazgos se han dividido: lesiones vasculares; lesiones inflamatorias; otras lesiones; estudios normales; estudios no concluyentes. Resultados De un total de 1.291 estudios se ha incluido 1.027 en el analisis. La edad media es 56,45 anos, con 471 hombres y 556 mujeres. La enfermedad mas frecuentemente observada fueron las lesiones vasculares, asociadas o no a otras lesiones. Cuando la indicacion era una hemorragia digestiva, el impacto diagnostico fue del 80%. En la enfermedad inflamatoria esta cifra solo alcanza el 50%. El rendimiento diagnostico es mucho menor en el grupo de «otras indicaciones». No se han registrado complicaciones mayores. Discusion La capsula de intestino delgado tiene un alto rendimiento diagnostico en los casos de hemorragia digestiva; el numero de estudios con hallazgos positivos es menor en los de enfermedad inflamatoria intestinal. Conclusiones Se trata de una modalidad diagnostica segura y de gran utilidad para el diagnostico de enfermedad del intestino delgado, aunque se precisa mejorar el indice de sospecha en la enfermedad inflamatoria intestinal.
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- 2017
39. O3 Impacto de la hiperglucemia y de la relación de la acción terapéutica de la metformina en la longitud de los telómeros
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Ariel Pablo Lopez, Andrea Elena Iglesias Molli, Gustavo Daniel Frechtel, María Amelia Linari, Alejandro de Dios, Gloria Edith Cerrone, Andrea Millán, and Constanza Pautasso
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Gynecology ,education.field_of_study ,medicine.medical_specialty ,business.industry ,Population ,Newly diagnosed ,Type 2 diabetes ,medicine.disease ,Metformin ,Pharmacological treatment ,Fasting glucose ,medicine ,General Earth and Planetary Sciences ,In patient ,business ,education ,After treatment ,General Environmental Science ,medicine.drug - Abstract
Introducción: la hiperglucemia es uno de los más importantes factores que determinan la producción de estrés oxidativo e inflamación, procesos que pueden acelerar el acortamiento de los telómeros.Objetivos: analizar la longitud telomérica absoluta (LTa) en individuos con niveles de glucosa en ayunas alterada (GAA) en comparación con individuos que presentan niveles normales de glucosa (GAN). Estudiar a partir de un estudio prospectivo controlado, la variación en la LTa en pacientes con DM2 descompensada antes y después del tratamiento.Materiales y métodos: se estudió una población de 246 individuos de ambos sexos, conformada por individuos con obesidad, individuos con DM2 o MODY e individuos controles sanos. Se dividieron en individuos con niveles de glucemia =110 mg/dl y 50Y). Se estudió un subgrupo de 30 pacientes con DM2 de reciente diagnóstico, al inicio (T0) y a los 6 meses (T6) de un tratamiento farmacológico y medidas higiénico-dietéticas, con el objetivo de lograr la compensación metabólica. En ambos estudios se determinaron las variables bioquímico-clínicas de todos los individuos y la LTa por PCR cuantitativa en tiempo real. Los análisis estadísticos se realizaron utilizando el software GraphPad Prism y SPSS.
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- 2020
40. Análisis de la longitud de los telómeros en obesos metabólicamente sanos
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Gustavo Daniel Frechtel, Gloria Edith Cerrone, Andrea Millán, Irma Slavutsky, Jorge Vilariño, Claudio Gonzalez, Andrea Elena Iglesias Molli, and Marta Sereday
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Síndrome metabólico ,Physics ,Telomere length ,Endocrinology, Diabetes and Metabolism ,Longitud telomérica ,030209 endocrinology & metabolism ,Metabolically healthy obese ,030204 cardiovascular system & hematology ,Metabolic syndrome ,Obesos metabólicamente sanos ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,Humanities - Abstract
ResumenLa obesidad es el principal componente del síndrome metabólico (SM) y determina la progresión de la enfermedad a las complicaciones metabólicas. Los individuos obesos metabólicamente sanos (OMS) parecen estar protegidos contra esas complicaciones. La longitud de los telómeros (LT) es un nuevo marcador del envejecimiento celular, que tiene una relación compleja con el SM. El objetivo principal de este estudio fue investigar por primera vez la LT en OMS y estudiar la asociación entre LT y el número de componentes del SM.Se estudió a 398 mujeres con una edad media de 46,76±15,47 años (rango: 18-86 años), que se agruparon en: individuos con normopeso sin ningún componente del SM (NP0), obesos sin SM (OMS) y de acuerdo con el número de componentes de SM en los grupos sin ningún componentes de SM (0), con uno o 2 componentes (1+2) y con SM por la presencia de 3 o más componentes (SM).La LT de los OMS no se diferenció de la de los NP0, pero fue significativamente mayor que la de los individuos con SM (p=0,032). Se observó una disminución de la LT con el aumento progresivo del número de componentes del SM (p=0,004), en donde el grupo 0 presentó una LT significativamente mayor que los grupos 1+2 (p=0,027) y SM (p=0,003).Demostramos por primera vez que las mujeres OMS presentan una LT similar a las mujeres NP0 y más larga que aquellas mujeres con SM. Además, confirmamos que la LT se acorta con el aumento en el número de alteraciones del SM.AbstractObesity is the principal component in Metabolic Syndrome (MetS) and determines the progression of metabolic complications. Metabolically healthy obese individuals (MHO) seem to be protected against those complications. Telomere length (TL), as a novel marker of cellular aging, has a complex relationship with MetS. The principal aim of this study was to investigate TL in MHO, and to study the association between TL and the number of MetS components.A study was conducted on 398 women (mean age: 46.76±15.47 years; range: 18 - 86 years), grouped according to the number of MetS components (0, 1+2, MetS), a group of normal-weight individuals with 0 MetS components (NW0), and a group of obese without MetS (MHO).No differences were found in the TL of the MHO group compared to the NW0, but it was significantly higher than that of individuals with MetS (P=.032). A decrease in TL was observed with a progressive increase in the number of MetS components (P=.004), whereas the group of individuals without MetS components had significantly longer TL than the groups with 1 and 2 components (P=.027), and MetS (P=.003).Shorter TL is not associated with MHO, but is related to MetS and with an increased number of metabolic abnormalities.
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- 2016
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41. Body mass index is negatively associated with telomere length: a collaborative cross-sectional meta-analysis of 87 observational studies
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Gil Atzmon, Rosalind Ramsey-Goldman, Barry J. McDonnell, Yun-Ling Zheng, David Gilley, Teresa E. Seeman, Maciej Tomaszewski, Massimo Mangino, Geja J. Hageman, Florian Kronenberg, Iiris Hovatta, Giuseppe Paolisso, Line Rode, Ellen W. Demerath, Jaakko Kaprio, Katarina Nordfjäll, Jonathan N. Hofmann, Peter Willeit, Rosana Maria dos Reis, Barbara Kollerits, Andrea Elena Iglesias Molli, Guo Cheng, Wei Zheng, Karen A. Mather, Daniel Eisenberg, Fadi J. Charchar, Sarah E. Harris, Bianca D’Antono, Jason Y.Y. Wong, Sofia Pavanello, Lisa A. Boardman, Guillaume Paré, Jacob B. H. Hjelmborg, Erik J. Giltay, Tim De Meyer, Evangelia Antoniou, Dmytro Krasnienkov, Maurice P. Zeegers, Marek Kasielski, Audrey E. Hendricks, Linda Broer, Lifang Hou, Andrew Steptoe, Andrew J. Pellatt, Jennifer A. Nettleton, Vanessa A. Diaz, Steven C. Hunt, Marij Gielen, Josine E. Verhoeven, Christian Gieger, Roxanne Schaakxs, Belinda L. Needham, Catherine Duggan, Timo E. Strandberg, Ute Mons, Klelia D. Salpea, Jeremy D. Kark, Raffaela Zannolli, Muthuswamy Balasubramanyam, Michael Chong, Richard P. Ebstein, Daniel Bunout, Amelia Marti, Kristina Sundquist, Lisa Mirabello, Hisham Nassar, David J. Kurz, Ramin Farzaneh-Far, Ilja Demuth, Pim van der Harst, Gielen, Marij, Hageman, Geja J., Antoniou, Evangelia E., Nordfjall, Katarina, Mangino, Massimo, Balasubramanyam, Muthuswamy, De Meyer, Tim, Hendricks, Audrey E., Giltay, Erik J., Hunt, Steven C., Nettleton, Jennifer A., Salpea, Klelia D., Diaz, Vanessa A., Farzaneh-Far, Ramin, Atzmon, Gil, Harris, Sarah E., Hou, Lifang, Gilley, David, Hovatta, Iiri, Kark, Jeremy D., Nassar, Hisham, Kurz, David J., Mather, Karen A., Willeit, Peter, Zheng, Yun-Ling, Pavanello, Sofia, Demerath, Ellen W., Rode, Line, Bunout, Daniel, Steptoe, Andrew, Boardman, Lisa, Marti, Amelia, Needham, Belinda, Zheng, Wei, Ramsey-Goldman, Rosalind, Pellatt, Andrew J., Kaprio, Jaakko, Hofmann, Jonathan N., Gieger, Christian, Paolisso, Giuseppe, Hjelmborg, Jacob B. H., Mirabello, Lisa, Seeman, Teresa, Wong, Jason, Van Der Harst, Pim, Broer, Linda, Kronenberg, Florian, Kollerits, Barbara, Strandberg, Timo, Eisenberg, Dan T. A., Duggan, Catherine, Verhoeven, Josine E., Schaakxs, Roxanne, Zannolli, Raffaela, Dos Reis, Rosana M. R., Charchar, Fadi J., Tomaszewski, Maciej, Mons, Ute, Demuth, Ilja, Molli, Andrea Elena Iglesia, Cheng, Guo, Krasnienkov, Dmytro, D'Antono, Bianca, Kasielski, Marek, Mcdonnell, Barry J., Ebstein, Richard Paul, Sundquist, Kristina, Pare, Guillaume, Chong, Michael, Zeegers, Maurice P., Complexe Genetica, RS: NUTRIM - R3 - Respiratory & Age-related Health, Farmacologie en Toxicologie, Section Eating Disorders and Obesity, RS: FPN CPS II, RS: CAPHRI - R5 - Optimising Patient Care, TELOMAAS Group, Internal Medicine, Psychiatry, APH - Mental Health, and Cardiovascular Centre (CVC)
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0301 basic medicine ,Male ,obesity ,Cross-sectional study ,Medicine (miscellaneous) ,BMI ,Low-grade inflammation ,Meta-analysis ,Obesity ,Observational studies ,Telomere length ,Nutrition and Dietetics ,030204 cardiovascular system & hematology ,Body Mass Index ,0302 clinical medicine ,Ethnicity ,Leukocytes ,telomere length ,low-grade inflammation ,Young adult ,Telomere Shortening ,2. Zero hunger ,Aged, 80 and over ,education.field_of_study ,INSULIN-RESISTANCE ,Age Factors ,Middle Aged ,Telomere ,OXIDATIVE DNA-DAMAGE ,Original Research Communications ,CARDIOVASCULAR-DISEASE ,STRONG HEART FAMILY ,CORONARY-ARTERY CALCIFICATION ,Adult ,Adolescent ,Population ,INFLAMAÇÃO ,03 medical and health sciences ,Insulin resistance ,Sex Factors ,Linear regression ,medicine ,Humans ,Meta-analysi ,education ,observational studies ,Aged ,CANCER RISK ,business.industry ,II BASE-II ,medicine.disease ,Observational studie ,AMERICAN-INDIANS ,meta-analysis ,030104 developmental biology ,Cross-Sectional Studies ,PULSE-WAVE VELOCITY ,HELSINKI BIRTH COHORT ,business ,Body mass index ,Demography - Abstract
Background: Even before the onset of age-related diseases, obesity might be a contributing factor to the cumulative burden of oxidative stress and chronic inflammation throughout the life course. Obesity may therefore contribute to accelerated shortening of telomeres. Consequently, obese persons are more likely to have shorter telomeres, but the association between body mass index (BMI) and leukocyte telomere length (TL) might differ across the life span and between ethnicities and sexes. Objective: A collaborative cross-sectionalmeta-analysis of observational studies was conducted to investigate the associations between BMI and TL across the life span. Design: Eighty-seven distinct study samples were included in the meta-analysis capturing data from 146,114 individuals. Studyspecific age- and sex-adjusted regression coefficients were combined by using a random-effects model in which absolute [base pairs (bp)] and relative telomere to single-copy gene ratio (T/S ratio) TLs were regressed against BMI. Stratified analysis was performed by 3 age categories ("young": 18-60 y; "middle": 61-75 y; and "old": >75 y), sex, and ethnicity. Results: Each unit increase in BMI corresponded to a-3.99 bp (95% CI: -5.17, -2.81 bp) difference in TL in the total pooled sample; among young adults, each unit increase in BMI corresponded to a -7.67 bp (95% CI:-10.03,-5.31 bp) difference. Each unit increase in BMI corresponded to a -1.58 × 10-3 unit T/S ratio (0.16% decrease; 95% CI: -2.14 × 10-3, -1.01 × 10-3) difference in ageand sex-adjusted relative TL in the total pooled sample; among young adults, each unit increase in BMI corresponded to a -2.58 × 10-3 unit T/S ratio (0.26% decrease; 95% CI: -3.92 × 10-3, -1.25 × 10-3). The associations were predominantly for the white pooled population. No sex differences were observed. Conclusions: A higher BMI is associated with shorter telomeres, especially in younger individuals. The presently observed difference is not negligible. Meta-analyses of longitudinal studies evaluating change in body weight alongside change in TL arewarranted.
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- 2018
42. Hepatic encephalopathy secondary to a splenorenal shunt that manifested a long time after a liver transplantation
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Andrés Francisco Jiménez Sánchez, Paula Tomás Pujante, Elena Iglesias Jorquera, and José Antonio Pons Miñano
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medicine.medical_specialty ,Cirrhosis ,Computed Tomography Angiography ,Vascular Malformations ,medicine.medical_treatment ,Liver transplantation ,Gastroenterology ,Renal Veins ,Postoperative Complications ,Internal medicine ,medicine ,Humans ,Hepatic encephalopathy ,Computed tomography angiography ,Aged ,medicine.diagnostic_test ,business.industry ,Hyperammonemia ,General Medicine ,medicine.disease ,Shunt (medical) ,Liver Transplantation ,Normal functioning ,surgical procedures, operative ,Splenic Vein ,Liver biopsy ,Hepatic Encephalopathy ,Female ,business - Abstract
Splenorenal shunts are a rare cause of hyperammonemia and hepatic encephalopathy in the absence of cirrhosis. We report the case of a woman, who presented hepatic encephalopathy, with a normal functioning graft, after 14 years of liver transplantation, confirmed by liver biopsy.
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- 2018
43. Inhibition of osteoblast function by Brucella abortus is reversed by dehydroepiandrosterone and involves ERK1/2 and estrogen receptor
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María Virginia Gentilini, Gloria Edith Cerrone, Guillermo H. Giambartolomei, María Victoria Delpino, Paula Constanza Arriola Benitez, Andrea Elena Iglesias Molli, and Ayelén Ivana Pesce Viglietti
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lcsh:Immunologic diseases. Allergy ,0301 basic medicine ,endocrine system ,medicine.medical_specialty ,CIENCIAS MÉDICAS Y DE LA SALUD ,Immunology ,Ciencias de la Salud ,Estrogen receptor ,Dehydroepiandrosterone ,DEHYDROEPIANDROSTERONE ,cortisol ,03 medical and health sciences ,purl.org/becyt/ford/3.3 [https] ,dehydroepiandrosterone ,0302 clinical medicine ,Glucocorticoid receptor ,Internal medicine ,Bone cell ,medicine ,Immunology and Allergy ,Secretion ,ADRENAL STEROIDS ,BRUCELLA ,Chemistry ,CORTISOL ,adrenal steroids ,Osteoblast ,bacterial infections and mycoses ,IMMUNOENDOCRINOLOGY ,Brucella ,Enfermedades Infecciosas ,030104 developmental biology ,Endocrinology ,medicine.anatomical_structure ,Apoptosis ,immunoendocrinology ,030220 oncology & carcinogenesis ,purl.org/becyt/ford/3 [https] ,Cortisone ,lcsh:RC581-607 ,hormones, hormone substitutes, and hormone antagonists ,medicine.drug - Abstract
Brucella abortus induces an inflammatory response that stimulates the endocrine system resulting in the secretion of cortisol and dehydroepiandrosterone (DHEA). Osteoarticular brucellosis is the most common presentation of the active disease in humans, and we have previously demonstrated that B. abortus infection inhibits osteoblast function. We aimed to evaluate the role of cortisol and DHEA on osteoblast during B. abortus infection. B. abortus infection induces apoptosis and inhibits osteoblast function. DHEA treatment reversed the effect of B. abortus infection on osteoblast by increasing their proliferation, inhibiting osteoblast apoptosis, and reversing the inhibitory effect of B. abortus on osteoblast differentiation and function. By contrast, cortisol increased the effect of B. abortus infection. Cortisol regulates target genes by binding to the glucocorticoid receptor (GR). B. abortus infection inhibited GRa expression. Cell responses to cortisol not only depend on GR expression but also on its intracellular bioavailability, that is, dependent on the activity of the isoenzymes 11β-hydroxysteroid dehydrogenase (HSD) type-1, 11β-HSD2 (which convert cortisone to cortisol and vice versa, respectively). Alterations in the expression of these isoenzymes in bone cells are associated with bone loss. B. abortus infection increased 11β-HSD1 expression but had no effect on 11β-HSD2. DHEA reversed the inhibitory effect induced by B. abortus infection on osteoblast matrix deposition in an estrogen receptor- and ERK1/2-dependent manner. We conclude that DHEA intervention improves osteoblast function during B. abortus infection making it a potential candidate to ameliorate the osteoarticular symptoms of brucellosis. Fil: Gentilini, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina Fil: Pesce Viglietti, Ayelén Ivana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina Fil: Arriola Benitez, Paula Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina Fil: Iglesias Molli, Andrea Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina Fil: Cerrone, Gloria Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina Fil: Giambartolomei, Guillermo Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina Fil: Delpino, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
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- 2018
44. Common and rare TBK1 variants in early-onset Alzheimer disease in a European cohort
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Juan Fortea, Marc Cruts, Tobi Van den Bossche, Jan Versijpt, Alessandro Padovani, Luisa Benussi, Roberta Ghidoni, Alexandre de Mendonça, Elena Lorenzo, Manuel Seijo-Martínez, Jaume Campdelacreu, Sandro Sorbi, Radoslav Matej, Peter Paul De Deyn, Panagiotis Alexopoulos, Olivier Deryck, Albert Lladó, Pau Pastor, Frederico Simões do Couto, Rik Vandenberghe, Estrella Gómez-Tortosa, Zdenek Rohan, Jordi Clarimón, Anne Sieben, Jordi Gascon, Isabel Santana, Maria A. Pastor, Maria Koutroumani, Madalena Martins, Sebastiaan Engelborghs, Janine Diehl-Schmid, Christine Van Broeckhoven, Benedetta Nacmias, Patrick Santens, Lubina Dillen, Ilse Gijselinck, Giuliano Binetti, Silvia Bagnoli, Bruno Bergmans, Agustín Ruiz, Raquel Sánchez-Valle, Barbara Borroni, Maria Rosário Almeida, Kristel Sleegers, Ellen Gelpi, Adrian Ivanoiu, Bavo Heeman, Cristina Razquin, Magda Tsolaki, Elena Iglesias, Caroline Graff, Johan Goeman, Alberto Lleó, Valentina Bessi, Laura Fratiglioni, Jan Verheijen, Alex Michotte, Jan De Bleecker, Rafael Blesa, Julie van der Zee, Eric Salmon, Ramón Reñé, Dirk Nuytten, Mathieu Vandenbulcke, Bart Dermaut, Håkan Thonberg, Christiana Willems, BELNEU Consortium, EU EOD Consortium, UCL - SSS/IONS/NEUR - Clinical Neuroscience, UCL - (SLuc) Service de neurologie, Clinical sciences, Neurology, and Pathologic Biochemistry and Physiology
- Subjects
0301 basic medicine ,Oncology ,Male ,Aging ,TBK1 ,Early onset Alzheimer's disease ,Frontotemporal dementia ,Loss-of-function ,RNA sequencing ,Neuroscience (all) ,Neurology (clinical) ,Developmental Biology ,Geriatrics and Gerontology ,Disease ,Bioinformatics ,medicine.disease_cause ,AMYOTROPHIC-LATERAL-SCLEROSIS ,Loss of Function Mutation/genetics ,Cohort Studies ,Frontotemporal Dementia/genetics ,0302 clinical medicine ,Loss of Function Mutation ,Medicine and Health Sciences ,Early-onset Alzheimer's disease ,Family history ,Amyotrophic lateral sclerosis ,Medicine(all) ,Mutation ,General Neuroscience ,Homozygote ,Amyotrophic Lateral Sclerosis/genetics ,Middle Aged ,Protein-Serine-Threonine Kinases ,3. Good health ,ddc ,Europe ,Genetic Variation/genetics ,Frontotemporal Dementia ,Cohort ,Female ,Risk ,medicine.medical_specialty ,Heterozygote ,BINDING KINASE 1 ,Protein-Serine-Threonine Kinases/genetics ,Neuroscience(all) ,Clinical Neurology ,IMMUNITY ,Biology ,Protein Serine-Threonine Kinases ,03 medical and health sciences ,Early onset Alzheimer’s disease ,Alzheimer Disease ,Internal medicine ,medicine ,Humans ,BELGIAN COHORT ,Loss function ,Alleles ,Genetic Association Studies ,Aged ,Alzheimer Disease/genetics ,MUTATIONS ,Amyotrophic Lateral Sclerosis ,Biology and Life Sciences ,Genetic Variation ,medicine.disease ,Ageing ,030104 developmental biology ,Human medicine ,030217 neurology & neurosurgery - Abstract
TANK-binding kinase 1 (TBK1) loss-of-function (LoF) mutations are known to cause frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS), often combined with memory deficits early in the disease course. We performed targeted resequencing of TBK1 in 1253 early onset Alzheimer's disease (EOAD) patients from 8 European countries to investigate whether pathogenic TBK1 mutations are enriched among patients with clinical diagnosis of EOAD. Variant frequencies were compared against 2117 origin-matched controls. We identified only 1 LoF mutation (p.Thr79del) in a patient clinically diagnosed with Alzheimer's disease and a positive family history of ALS. We did not observe enrichment of rare variants in EOAD patients compared to controls, nor of rare variants affecting NFκB induction. Of 3 common coding variants, rs7486100 showed evidence of association (OR 1.46 [95% CI 1.13-1.9]; p-value 0.01). Homozygous carriers of the risk allele showed reduced expression of TBK1 (p-value 0.03). Our findings are not indicative of a significant role for TBK1 mutations in EOAD. The association between common variants in TBK1, disease risk and reduced TBK1 expression warrants follow-up in FTD/ALS cohorts. ispartof: Neurobiology of Aging vol:62 pages:245- ispartof: location:United States status: published
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- 2018
45. Síndrome nefrótico por gammapatía monoclonal de significado renal
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María Crucio López, J. A. Méndez Sánchez, Beatriz Ferreiro, Elena Iglesias, and Alfonso Otero González
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Nephrology - Published
- 2019
46. Litio. Eutimizante y potencial nefrotóxico
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Beatriz San Millán, Alfonso Otero González, Enrique Novoa Fernández, M Camba, María Pia Curcio, Cristina Fernández Pérez, Alfonso Iglesias Forneiro, Beatriz Ferreiro, Elena Iglesias, and Lorena Rivera
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Nephrology - Published
- 2019
47. Metabolically healthy obese individuals present similar chronic inflammation level but less insulin-resistance than obese individuals with metabolic syndrome
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Jorge Vilariño, Gloria Edith Cerrone, Andrea Elena Iglesias Molli, Alberto Penas Steinhardt, Ariel Pablo Lopez, Claudio Gonzalez, and Gustavo Daniel Frechtel
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Male ,Physiology ,medicine.medical_treatment ,Adipose tissue ,lcsh:Medicine ,Cardiovascular Medicine ,030204 cardiovascular system & hematology ,Pathology and Laboratory Medicine ,Biochemistry ,Body Mass Index ,0302 clinical medicine ,Glucose Metabolism ,Immune Physiology ,Medicine and Health Sciences ,lcsh:Science ,Immune Response ,Metabolic Syndrome ,Innate Immune System ,education.field_of_study ,Multidisciplinary ,biology ,purl.org/becyt/ford/3.1 [https] ,Medicina Básica ,Physiological Parameters ,Adipose Tissue ,Cardiovascular Diseases ,Carbohydrate Metabolism ,Cytokines ,purl.org/becyt/ford/3 [https] ,Female ,Anatomy ,Research Article ,Adult ,medicine.medical_specialty ,CIENCIAS MÉDICAS Y DE LA SALUD ,Immunology ,Population ,Genética Humana ,030209 endocrinology & metabolism ,Young Adult ,03 medical and health sciences ,Signs and Symptoms ,Insulin resistance ,Diagnostic Medicine ,Internal medicine ,medicine ,Humans ,Obesity ,education ,Inflammation ,business.industry ,Insulin ,Body Weight ,C-reactive protein ,lcsh:R ,Biology and Life Sciences ,Molecular Development ,medicine.disease ,Biological Tissue ,Metabolism ,Endocrinology ,Metabolic Disorders ,Immune System ,Chronic Disease ,biology.protein ,lcsh:Q ,Insulin Resistance ,Metabolic syndrome ,business ,Body mass index ,Developmental Biology - Abstract
The Metabolic Syndrome (MetS) is a cluster of cardiometabolic risk factors, usually accompanied by the presence of insulin resistance (IR) and a systemic subclinical inflammation state. Metabolically healthy obese (MHO) individuals seem to be protected against cardiometabolic complications. The aim of this work was to characterize phenotypically the low-grade inflammation and the IR in MHO individuals in comparison to obese individuals with MetS and control non obese. We studied two different populations: 940 individuals from the general population of Buenos Aires and 518 individuals from the general population of Venado Tuerto; grouped in three groups: metabolically healthy non-obese individuals (MHNO), MHO and obese individuals with MetS (MSO). Inflammation was measured by the levels of hs-CRP (high-sensitivity C reactive protein), and we found that MHO presented an increase in inflammation when compared with MHNO (Buenos Aires: p
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- 2017
48. Inhibition of Osteoblast Function by
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María Virginia, Gentilini, Ayelén Ivana, Pesce Viglietti, Paula Constanza, Arriola Benitez, Andrea Elena, Iglesias Molli, Gloria Edith, Cerrone, Guillermo Hernán, Giambartolomei, and María Victoria, Delpino
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endocrine system ,Immunology ,Brucella abortus ,Gene Expression ,Apoptosis ,cortisol ,Cell Line ,Brucellosis, Bovine ,Mice ,Receptors, Glucocorticoid ,dehydroepiandrosterone ,Osteogenesis ,Animals ,Cell Proliferation ,Original Research ,Mitogen-Activated Protein Kinase 1 ,Microbial Viability ,Mitogen-Activated Protein Kinase 3 ,Osteoblasts ,adrenal steroids ,Cell Differentiation ,bacterial infections and mycoses ,Brucella ,Receptors, Estrogen ,immunoendocrinology ,Cattle ,hormones, hormone substitutes, and hormone antagonists ,Biomarkers - Abstract
Brucella abortus induces an inflammatory response that stimulates the endocrine system resulting in the secretion of cortisol and dehydroepiandrosterone (DHEA). Osteoarticular brucellosis is the most common presentation of the active disease in humans, and we have previously demonstrated that B. abortus infection inhibits osteoblast function. We aimed to evaluate the role of cortisol and DHEA on osteoblast during B. abortus infection. B. abortus infection induces apoptosis and inhibits osteoblast function. DHEA treatment reversed the effect of B. abortus infection on osteoblast by increasing their proliferation, inhibiting osteoblast apoptosis, and reversing the inhibitory effect of B. abortus on osteoblast differentiation and function. By contrast, cortisol increased the effect of B. abortus infection. Cortisol regulates target genes by binding to the glucocorticoid receptor (GR). B. abortus infection inhibited GRα expression. Cell responses to cortisol not only depend on GR expression but also on its intracellular bioavailability, that is, dependent on the activity of the isoenzymes 11β-hydroxysteroid dehydrogenase (HSD) type-1, 11β-HSD2 (which convert cortisone to cortisol and vice versa, respectively). Alterations in the expression of these isoenzymes in bone cells are associated with bone loss. B. abortus infection increased 11β-HSD1 expression but had no effect on 11β-HSD2. DHEA reversed the inhibitory effect induced by B. abortus infection on osteoblast matrix deposition in an estrogen receptor- and ERK1/2-dependent manner. We conclude that DHEA intervention improves osteoblast function during B. abortus infection making it a potential candidate to ameliorate the osteoarticular symptoms of brucellosis.
- Published
- 2017
49. 2167 - EVALUACIÓN DE PRUEBAS DE DETECCIÓN Y CARACTERÍSTICAS DEMOGRÁFICAS DE LA FARINGITIS POR S. PYOGENESEN EL PRIMER TRIMESTRE DEL AÑO 2022 Y 2023, EN LA UNIDAD DE URGENCIAS DEL HOSPITAL UNIVERSITARIO DEL HENARES
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Morante, Daniel Alejandro García, Rincon, Erika Esperanza Lozano, Leon, Juan Francisco Bayona, Santiago, Jose Ángel Pestaña, Saleh, Wafa Elgeadi, Gil, Elena Iglesias, Muñoz, Carlota Madrigal, and Colino, Laura Larrin
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- 2023
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50. Rituximab for Steroid-Dependent or Frequently Relapsing Idiopathic Nephrotic Syndrome in Adults: A Retrospective, Multicenter Study in Spain
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Juliana Draibe, Manuel Praga, Iara daSilva, Montserrat Díaz-Encarnación, José Ballarín, Beatriz Redondo, Luis F. Quintana, Elena Iglesias, and Ana Huerta
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Adult ,Male ,medicine.medical_specialty ,Nephrotic Syndrome ,Adolescent ,030232 urology & nephrology ,030204 cardiovascular system & hematology ,Gastroenterology ,Tacrolimus ,Antibodies, Monoclonal, Murine-Derived ,Young Adult ,03 medical and health sciences ,Antineoplastic Agents, Immunological ,0302 clinical medicine ,Pharmacotherapy ,Recurrence ,immune system diseases ,Internal medicine ,hemic and lymphatic diseases ,medicine ,Humans ,Pharmacology (medical) ,Young adult ,Child ,Aged ,Retrospective Studies ,Pharmacology ,Proteinuria ,business.industry ,Incidence (epidemiology) ,Infant ,Retrospective cohort study ,General Medicine ,Middle Aged ,medicine.disease ,Endocrinology ,Spain ,Child, Preschool ,Female ,Steroids ,Rituximab ,medicine.symptom ,business ,Nephrotic syndrome ,Immunosuppressive Agents ,Biotechnology ,medicine.drug - Abstract
Background Patients with difficult-to-treat idiopathic nephrotic syndrome (INS), steroid-dependent nephrotic syndrome (SDNS), or frequently relapsing nephrotic syndrome (FRNS) require long-term immunosuppressive therapy. Rituximab offers an alternative treatment for patients with disease that has not responded to multiple therapies. Objective Our objective was to determine the efficacy and safety of rituximab in adult patients with difficult-to-treat (SDNS or FRNS) INS. Methods We performed a retrospective multicenter study that included 50 adults with difficult-to-treat INS in six Spanish centers. All patients were treated with steroids in combination with another immunosuppressant: 28 patients received rituximab as the additional treatment (rituximab group), and the other 22 patients not treated with rituximab served as the control group. Results Of the patients treated with rituximab, 23 (82%) experienced complete remission, 20 (71%) had no relapses after receiving rituximab, and 13 (46%) did not receive any immunosuppressant. Of those in the control group, 14 (63%) experienced complete remission, including eight without immunosuppressants (29%). The rituximab group experienced highly significant reductions in total number of relapses per year (p < 0.001), proteinuria (p = 0.03), steroid doses (p = 0.002), and tacrolimus doses (p = 0.001). Mean follow-up after rituximab was 31 +/- 26 months (range 8-86). The need for steroids and other immunosuppressants to achieve sustained remission was lower in the rituximab group than in the control group. Conclusions Rituximab treatment was safe and well tolerated. It effectively reduced the incidence of relapses and need for maintenance immunosuppressive therapy in adults with difficult-to-treat INS.
- Published
- 2017
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