Search

In times of coronavirus disease 2019 (COVID-19), the impact of severe acute respiratory syndrome (SARS)-coronavirus (CoV)-2 infection on pregnancy is still unclear. The presence of angiotensin-converting enzyme (ACE) 2 (ACE2), the main receptor for SARS-CoV-2, in human placentas indicates that this organ can be vulnerable for viral infection during pregnancy. However, for this to happen, additional molecular processes are critical to allow viral entry in cells, its replication and disease manifestation, particularly in the placenta and/or feto-maternal circulation. Beyond the risk of vertical transmission, COVID-19 is also proposed to deplete ACE2 protein and its biological actions in the placenta. It is postulated that such effects may impair essential processes during placentation and maternal hemodynamic adaptations in COVID-19 pregnancy, features also observed in several disorders of pregnancy. This review gathers information indicating risks and protective features related to ACE2 changes in COVID-19 pregnancies. First, we describe the mechanisms of SARS-CoV-2 infection having ACE2 as a main entry door and current evidence of viral infection in the placenta. Further, we discuss the central role of ACE2 in physiological systems such as the renin-angiotensin system (RAS) and the kallikrein-kinin system (KKS), both active during placentation and hemodynamic adaptations of pregnancy. Significant knowledge gaps are also identified and should be urgently filled to better understand the fate of ACE2 in COVID-19 pregnancies and the potential associated risks. Emerging knowledge will be able to improve the early stratification of high-risk pregnancies with COVID-19 exposure as well as to guide better management and follow-up of these mothers and their children. [ABSTRACT FROM AUTHOR]

Background: G-CSF is a critical regulator of hematopoietic cell proliferation, differentiation and survival. It has been reported that G-CSF attenuates renal injury during acute ischemia-reperfusion. In this study we evaluated the effects of G-CSF on the renal and cardiovascular systems of 2K1C hypertensive mice. Methods: Male C57BL/6 mice were subjected to left renal artery clipping (2K1C) or sham operation and were then administered G-CSF (100 μg/kg/day) or vehicle for 14 days. Results: Arterial pressure was higher in 2K1C + vehicle animals than in 2K1C + GCSF (150±5 vs. 129±2 mmHg, p<0.01, n=8). Plasma angiotensin I, II and 1-7 concentrations were significantly increased in 2K1C + Vehicle when compared to the normotensive Sham group. G-CSF prevented the increase of these vasoactive peptides. The clipped kidney/contralateral kidney weight ratio showed a less atrophy of the ischemic kidney in the treated group (0.50±0.02 vs. 0.66±0.01, p<0.05). The infarction area in the clipped kidney was completely prevented in 7 out of 8 2K1C + G-CSF mice. Administration of G-CSF protected the clipped kidney from apoptosis. Conclusion: Our data indicate that G-CSF prevents kidney infarction and markedly attenuates the increases in plasma angiotensin levels and hypertension in 2K1C mice, reinforcing the protective effect of G-CSF on kidney ischemia. [ABSTRACT FROM AUTHOR]

R449 --> Childhood obesity is a complex multifactorial disease that contributes to higher cardiometabolic risk. Other important parameter is the pattern of fat distribution, even among normal weight children, those with central fat deposition show higher cardiovascular risk. The waist to height ratio (WHtR) is a useful and practical tool to assess central obesity and cardiovascular risk. Childhood obesity is associated with metabolic abnormalities which are mechanisms underlying cardiovascular diseases. The metabolomics can detect biomarkers for exposure, disease progression and susceptibility contributing to early intervention strategies. In this cross‐sectional study it was evaluated the profile of amino acids in urine of children with different cardiovascular risk status according to WHtR. The 110 participants aged from 9 to 10 years were classified according to WHtR into the groups: high cardiovascular risk (n = 48) and normal cardiovascular risk (n = 62). The 24 hours urine samples were collected, and aliquots submitted to LC–MS/MS for evaluation of 47 amino acids using the Amino Acids Analysis Kit (Zivak®, Turkey), values were corrected by creatinine. Biochemical profile, cardiovascular parameters and anthropometric measurements were assessed following standard protocols. The following amino acids were found in higher concentrations in urine of children from high cardiovascular risk group, cystathionine (25 vs 21 nmol/mg of creatinine, p = 0.03), Lys (212 vs 160, p < 0.01) and the aromatic amino acids Tyr (131 vs 90, p < 0.01), Trp (109 vs 95, p =0.02) and Phe (65 vs 46, p = 0.04). Linear regression identified that cystathionine, Lys, Tyr, Trp and Phe presented weak but significant positive correlation with anthropometric measurements. Cystathionine, Trp and Lys negatively correlates with HDL while Phe and Tyr positively correlated with triglycerides. Also, Lys and Tyr were positively associated with higher systolic blood pressure. Findings corroborate to literature and indicate that there is a signature profile of amino acids in urine of children with increased cardiovascular risk associated to central obesity. The augmented Tyr and Lys may be the most significant markers once they were also associated to higher SBP. [ABSTRACT FROM AUTHOR]