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Your search keyword '"Elena Bottazzi, Maria"' showing total 7 results
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7 results on '"Elena Bottazzi, Maria"'

2. A novel multi-epitope recombinant protein elicits an antigen-specific CD8+ T cells response in Trypanosoma cruzi-infected mice

Author

González-López, Cristina, primary, Chen, Wen-Hsiang, additional, Alfaro-Chacón, Andrea, additional, Villanueva-Lizama, Liliana E., additional, Rosado-Vallado, Miguel, additional, Ramirez-Sierra, Maria Jesús, additional, Teh-Poot, Christian F., additional, Pollet, Jeroen, additional, Asojo, Oluwatoyin, additional, Jones, Kathryn M., additional, Hotez, Peter J., additional, Elena Bottazzi, Maria, additional, and Cruz-Chan, Julio Vladimir, additional

Published
2022
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3. Receptor-binding domain recombinant protein on alum-CpG induces broad protection against SARS-CoV-2 variants of concern

Author

Pollet, Jeroen, primary, Strych, Ulrich, additional, Chen, Wen-Hsiang, additional, Versteeg, Leroy, additional, Keegan, Brian, additional, Zhan, Bin, additional, Wei, Junfei, additional, Liu, Zhuyun, additional, Lee, Jungsoon, additional, Kundu, Rahki, additional, Adhikari, Rakesh, additional, Poveda, Cristina, additional, Jose Villar, Maria, additional, Rani Thimmiraju, Syamala, additional, Lopez, Brianna, additional, Gillespie, Portia M., additional, Ronca, Shannon, additional, Kimata, Jason T., additional, Reers, Martin, additional, Paradkar, Vikram, additional, Hotez, Peter J., additional, and Elena Bottazzi, Maria, additional

Published
2022
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4. 2257

Author

Briggs, Neima, primary, Versteeg, Leroy, additional, Zhan, Bin, additional, Mejia, Rojelio, additional, Keegan, Brian, additional, Beaumier, Coreen, additional, Sastry, Jagannadha, additional, Elena Bottazzi, Maria, additional, and Hotez, Peter, additional

Published
2017
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5. Optimization and revision of the production process of the Necator americanus glutathione S-transferase 1 (Na-GST-1), the lead hookworm vaccine recombinant protein candidate

Author

Curti, Elena, primary, Seid, Christopher A, additional, Hudspeth, Elissa, additional, Center, Lori, additional, Rezende, Wanderson, additional, Pollet, Jeroen, additional, Kwityn, Cliff, additional, Hammond, Molly, additional, Matsunami, Rise K, additional, Engler, David A, additional, Hotez, Peter J, additional, and Elena Bottazzi, Maria, additional

Published
2014
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7. Optimization and revision of the production process of the Necator americanusglutathione S-transferase 1 (Na-GST-1), the lead hookworm vaccine recombinant protein candidate

Author

Curti, Elena, Seid, Christopher A, Hudspeth, Elissa, Center, Lori, Rezende, Wanderson, Pollet, Jeroen, Kwityn, Cliff, Hammond, Molly, Matsunami, Rise K, Engler, David A, Hotez, Peter J, and Elena Bottazzi, Maria

Abstract

Infection by the human hookworm Necator americanusis a leading cause of anemia and disability in the developing countries of Africa, Asia, and the Americas. In order to prevent childhood hookworm disease in resource poor settings, a recombinant vaccine is under development by the Sabin Vaccine Institute and Texas Children’s Hospital Center for Vaccine Development, a Product Development Partnership (PDP). Previously, we reported on the expression and purification of a highly promising hookworm vaccine candidate, Na-GST-1, an N. americanusglutathione s-transferase expressed in Pichia pastoris(yeast), which led to production of 1.5 g of 95% pure recombinant protein at a 20L scale.1, 2, 3This yield and purity of Na-GST-1 was sufficient for early pilot manufacturing and initial phase 1 clinical testing. However, based on the number of doses which would be required to allow mass vaccination and a potential goal to deliver a vaccine as inexpensively as possible, a higher yield of expression of the recombinant antigen at the lowest possible cost is highly desirable. Here we report on modifications to the fermentation (upstream process) of the antigen expressed in P. pastoris,and to the purification (downstream process) of the recombinant protein that allowed for a 2–3-fold improvement in the final yield of Na-GST-1 purified protein. The major improvements included upstream process changes such as the addition of a sorbitol pulse and co-feed during methanol induction as well as an extension of the induction stage to approximately 96 hours; downstream process changes included modifying the UFDF to flat sheet with a 10 kDa Molecular Weight cut-off (MWCO), adjusting the capacity of an ion-exchange chromatography step utilizing a gradient elution as opposed to the original step elution, and altering the hydrophobic interaction chromatography conditions. The full process, as well as the purity and stability profiles of the target Na-GST-1, and its formulation on Alhydrogel®, is described.

Published
2014
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