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2. Nucleic Acid Nanotechnology

Author

Jørgen Kjems, Elena Ferapontova, Kurt V. Gothelf, Jørgen Kjems, Elena Ferapontova, and Kurt V. Gothelf

Subjects
Nanochemistry, Gene expression, Nucleic acids, Nanotechnology, Cytology
Abstract

This volume on nucleic acid nanotechnology offers authoritative, up-to-date and comprehensive coverage of nanotechnological studies and applications of nucleic acids. It provides reviews of various aspects of nucleic acid nanotechnology, each written by an internationally leading expert in the field, and presents state-of-the-art and recent advances in nucleic acid synthetic modifications, nanoscale design, manipulation and current and future applications in bioengineering, medicine, electronics, genetic analysis, chemistry, molecular biology, surface and material sciences. It examines how nucleic acid research is merging with nanotechnology, allowing the nanoscale properties of nucleic acid to be exploited in performing challenging nanotechnological tasks, from nanorobotics and nanosensing to nucleic acid computing. This book will above all benefit anyone who is interested in nanotechnological concepts of nucleic acid design and applications, and offers a valuable resource for teaching these concepts. It is essential reading for a broad audience of scientists both in academia and industry who wish to expand their expertise on the potential of nucleic acid functions and applications.

Published
2014

7. A DNA nanoswitch incorporating the fluorescent base analogue 2-aminopurine detects single nucleotide mismatches in unlabelled targetsElectronic supplementary information (ESI) available: Fig. S1, emission spectra of AP with and without Mg2+; Fig. S2, excitation spectra of AP with and without Mg2+; Fig. S3, excitation spectra of nanoswitches with and without Mg2+; Fig. S4, time-resolved data for all open nanoswitches; Fig. S5, time-resolved data for all closed nanoswitches; Fig. S6, proportional change in time-resolved emission for all nanoswitches; Table S1, time-resolved emission data. See DOI: 10.1039/b900325h

Author

Colin J. Campbell, Christopher P. Mountford, Helene C. Stoquert, Amy H. Buck, Paul Dickinson, Elena Ferapontova, Jonathan G. Terry, John S. Beattie, Anthony J. Walton, Jason Crain, Peter Ghazal, and Andrew R. Mount

Subjects
FLUORESCENCE, NUCLEOTIDES, GENE targeting, NUCLEIC acid probes, DNA, NANOSTRUCTURED materials, FLUORESCENCE spectroscopy, MOLECULAR biology techniques
Abstract

DNA nanoswitches can be designed to detect unlabelled nucleic acid targets and have been shown to discriminate between targets which differ in the identity of only one base. This paper demonstrates that the fluorescent base analogue 2-aminopurine (AP) can be used to discriminate between nanoswitches with and without targets and to discriminate between matched and mismatched targets. In particular, we have used both steady-state and time-resolved fluorescence spectroscopy to determine differences in AP environment at the branchpoint of nanoswitches assembled using complementary targets and targets which incorporate single base mismatches. [ABSTRACT FROM AUTHOR]

Published
2009
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