Search

Food contamination by foodborne pathogens is considered a serious problem worldwide. This study aimed to show the efficacy of the one-pot green biosynthesis of nanocomposites as effective antimicrobial agents based on a water-soluble biodegradable polysaccharide and silver nitrate (AgNO3). Silver (Ag) nanoparticles were synthesized using different concentrations of AgNO3 solution (1, 2, and 3 mM) in the presence of N-quaternized chitosan and N,N,N-trimethyl chitosan chloride (TMC) as both a reducing and stabilizing agent. In addition, the structure of TMC/Ag nanocomposites was confirmed using different analytical tools including FTIR, UV-Vis, XRD, HR-TEM, FE-SEM, and EDX techniques. The FTIR spectra and UV-Vis spectra showed the main characteristic absorption peaks of Ag nanoparticles. In addition, FE-SEM images showed the formation of spherical bead-like particles on the surface of TMC. Correspondingly, the EDX spectrum showed a peak for silver, indicating the successful synthesis of Ag nanoparticles inside the TMC chains. Moreover, HR-TEM images exhibited the good distribution of Ag nanoparticles, which appeared as nano-spherical shapes. The antimicrobial activity of TMC/Ag nanocomposites was examined against three foodborne pathogens, including Salmonella Typhimurium as a Gram-negative bacterium, Bacillus subtilis as a Gram-positive bacterium and Aspergillus fumigatus as a fungus. The results showed that TMC/Ag nanocomposites had better antimicrobial activity compared with TMC alone and their antimicrobial activity increased with an increase in the concentration of Ag. The results confirmed that the TMC/Ag nanocomposites can be potentially used as an effective antimicrobial agent in food preservation.