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196 results on '"Elbuken, Caglar"'

10. From ex ovo to in vitro: xenotransplantation and vascularization of mouse embryonic kidneys in a microfluidic chip.

Author

Oliveira, Micaela, Sarker, Partha Protim, Skovorodkin, Ilya, Kalantarifard, Ali, Haskavuk, Tugce, Mac Intyre, Jonatan, Nallukunnel Raju, Elizabath, Nooranian, Samin, Shioda, Hiroki, Nishikawa, Masaki, Sakai, Yasuyuki, Vainio, Seppo J., Elbuken, Caglar, and Raykhel, Irina

Subjects
ORGANS (Anatomy), MORPHOLOGY, LABS on a chip, CHORIOALLANTOIS, BLOOD filtration
Abstract

Organoids are emerging as a powerful tool to investigate complex biological structures in vitro. Vascularization of organoids is crucial to recapitulate the morphology and function of the represented human organ, especially in the case of the kidney, whose primary function of blood filtration is closely associated with blood circulation. Current in vitro microfluidic approaches have only provided initial vascularization of kidney organoids, whereas in vivo transplantation to animal models is problematic due to ethical problems, with the exception of xenotransplantation onto a chicken chorioallantoic membrane (CAM). Although CAM can serve as a good environment for vascularization, it can only be used for a fixed length of time, limited by development of the embryo. Here, we propose a novel lab on a chip design that allows organoids of different origin to be cultured and vascularized on a CAM, as well as to be transferred to in vitro conditions when required. Mouse embryonic kidneys cultured on the CAM showed enhanced vascularization by intrinsic endothelial cells, and made connections with the chicken vasculature, as evidenced by blood flowing through them. After the chips were transferred to in vitro conditions, the vasculature inside the organoids was successfully maintained. To our knowledge, this is the first demonstration of the combination of in vivo and in vitro approaches applied to microfluidic chip design. [ABSTRACT FROM AUTHOR]

Published
2024
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12. Reply to Kaestner et al.: Pioneering quantitative platforms for stored red blood cell assessment open the door for precision transfusion medicine

Author

Isiksacan, Ziya, primary, D’Alessandro, Angelo, additional, McKenna, David H., additional, Tessier, Shannon N., additional, Kucukal, Erdem, additional, Gokaltun, A. Aslihan, additional, William, Nishaka, additional, Sandlin, Rebecca D., additional, Bischof, John, additional, Mohandas, Narla, additional, Busch, Michael P., additional, Elbuken, Caglar, additional, Gurkan, Umut A., additional, Toner, Mehmet, additional, Acker, Jason P., additional, Yarmush, Martin L., additional, and Usta, O. Berk, additional

Published
2024
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14. Scalable Purification, Storage, and Release of Plant-Derived Nanovesicles for Local Therapy Using Nanostructured All-Cellulose Composite Membranes

Author

Pratiwi, Feby W., Thomas, Reny T., Karzarjeddi, Mohammad, Sarpola, Marjaana, Miinalainen, Ilkka, Makieieva, Olha, Jokipii-Lukkari, Soile, Elbuken, Caglar, Oksman, Kristiina, Vainio, Seppo J., and Liimatainen, Henrikki

Abstract

Plant-derived nanovesicles such as bilberries nanovesicles (BNVs) show immense promise as next-generation biotherapeutics and functional food ingredients; however, their isolation, purification, and storage on a large scale remain a challenge. In this study, biocompatible and nanostructured composite all-cellulose membranes are introduced as a scalable and straightforward approach to the isolation of BNV. The membranes consisting of a cellulose acetate matrix infused with anionic or cationic nanocelluloses promoted selective capture of BNVs through electrostatic and size-exclusion-mediated depth filtration. Furthermore, the surface of the composite membrane acted as a storage matrix for BNVs, ensuring their prolonged stability at 4 °C. The BNVs stored in the membrane could be promptly released through elution assisted by low-pressure vacuum filtration or diffusion in liquid media. The morphology, bioactivity, and stability of the extracted BNVs were preserved, and the release rate of BNVs in different cell cultures could be regulated, facilitating their use for local therapy. Consequently, this approach paves the way for the scalable production, purification, and storage of nanovesicles and advances their use in biotherapeutics and functional foods.

Published
2024
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20. Anomalous electrohydrodynamic cross-stream particle migration.

Author

Abdorahimzadeh, Seyedamirhosein, Bölükkaya, Zikrullah, Vainio, Seppo J., Liimatainen, Henrikki, and Elbuken, Caglar

Subjects
LIFT (Aerodynamics), AQUEOUS electrolytes, ELECTROLYTE solutions, LATERAL loads, FLUID flow
Abstract

Particle cross-stream migration in electrohydrodynamic microfluidic systems exhibits intriguing behaviors, which makes it interesting when viewed from a fundamental perspective and promising for nanoparticle focusing and separation applications. So far, particle behavior in such systems has been explained with the slip-induced lift force model (Saffman model), which predicts particle central or side focusing based on the direction of electric field and fluid flow. However, in our previous work, we observed particle migration patterns that did not adhere to the prediction of the Saffman model. In this work, we further studied this novel particle lateral migration behavior, which we termed the "anti-Saffman" behavior. We experimentally investigated how changing the conductivity of the suspending medium influences particle behavior and quantitatively measured the net lateral force experienced by the particles. Then, we compared this net force with the prediction of the relevant lift force models in the literature. We concluded that the anti-Saffman behavior is positively correlated with medium conductivity and shear rate (∝ γ ̇ 2 ). Furthermore, the comparison with the existing force models revealed that none of them can predict the experimentally observed particle lift. The net lift predicted by hydrodynamic lift models indicated that the underlying mechanism behind our experiments also potentially has a hydrodynamic origin. We believe this phenomenon offers the possibility of manipulating and separating nanoparticles suspended in standard aqueous electrolyte solutions, which makes it applicable to various biological samples. [ABSTRACT FROM AUTHOR]

Published
2024
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21. Assessment of stored red blood cells through lab-on-a-chip technologies for precision transfusion medicine

Author

Isiksacan, Ziya, primary, D’Alessandro, Angelo, additional, Wolf, Susan M., additional, McKenna, David H., additional, Tessier, Shannon N., additional, Kucukal, Erdem, additional, Gokaltun, A. Aslihan, additional, William, Nishaka, additional, Sandlin, Rebecca D., additional, Bischof, John, additional, Mohandas, Narla, additional, Busch, Michael P., additional, Elbuken, Caglar, additional, Gurkan, Umut A., additional, Toner, Mehmet, additional, Acker, Jason P., additional, Yarmush, Martin L., additional, and Usta, O. Berk, additional

Published
2023
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29. Macroporous Surgical Mesh from a Natural Cocoon Composite

Author

Chen, Yong-Ming, primary, Zang, Lian-Sheng, additional, Koc-Bilican, Behlul, additional, Bilican, Ismail, additional, Holland, Chris, additional, Cansaran-Duman, Demet, additional, Karaduman, Tugce, additional, Çolak, Arzu, additional, Bayır, Yasin, additional, Halici, Zekai, additional, Ozmen, Sevilay, additional, Ali, Asad, additional, Labidi, Jalel, additional, Elbuken, Caglar, additional, and Kaya, Murat, additional

Published
2022
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31. Xenogenic Neural Stem Cell‐Derived Extracellular Nanovesicles Modulate Human Mesenchymal Stem Cell Fate and Reconstruct Metabolomic Structure

Author

Derkus, Burak, primary, Isik, Melis, additional, Eylem, Cemil Can, additional, Ergin, Irem, additional, Camci, Can Berk, additional, Bilgin, Sila, additional, Elbuken, Caglar, additional, Arslan, Yavuz Emre, additional, Akkulak, Merve, additional, Adali, Orhan, additional, Kiran, Fadime, additional, Okesola, Babatunde O., additional, Nemutlu, Emirhan, additional, and Emregul, Emel, additional

Published
2022
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35. Modeling and analysis of eddy-current damping for high-precision magnetic levitation of a small magnet

Author

Elbuken, Caglar, Shameli, Ehsan, and Khamesee, Mir Behrad

Subjects
Eddy currents (Electric) -- Control, Eddy currents (Electric) -- Research, Magnetic levitation vehicles -- Research, Business, Electronics, Electronics and electrical industries
Abstract

This paper presents modeling and analysis of eddy-current damping that is formed by a conductive plate placed below the levitating object in order to suppress vibrations and ensure stability. It is demonstrated that vibrations should be damped to preserve stability and precision especially for stepwise motion. The levitated object is a small permanent magnet in our experiments. A magnetic drive unit is used for vertical motion of the magnet. Eddy-current distribution in the plate is calculated by solving diffusion equation for vector magnetic potential. The eddy force applied to the object is derived by a coil model representation. It is shown that if a 20 mm radius, 9 mm thick aluminum circular plate is used for eddy-current damping, the levitated object can closely follow a step input with a steady-state precision varying between 0.04 and 0.07 mm depending on the plate object distance. Eddy-current damping is a key technique that improves levitation performance to increase the diversity of applications of magnetic levitation systems in micromanipulation and microelectronic fabrication. Index Terms--Eddy current, eddy-current damping, magnetic levitation, mechatronics, modeling.

Published
2007

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