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Your search keyword '"El-Zemaity, Mohamed S"' showing total 3 results
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3 results on '"El-Zemaity, Mohamed S"'

1. Isolation and Characterization of Native Entomopathogenic Fungi and Their Pathogenicity Against Cowpea aphid.

Author

Elsawy, Alyaa M, El-Zemaity, Mohamed S, Ahmed, Yosra, and El-Sayed, Walaa

Subjects
*ENTOMOPATHOGENIC fungi, *ECOSYSTEMS, *METARHIZIUM, *BEAUVERIA, *PESTICIDES
Abstract

The research aimed to isolate and characterize local entomopathogenic fungi from different agricultural ecosystems and assess their pathogenicity against cowpea aphid. Forty soil samples were collected from different ecosystems. In total, 38 fungal isolates were collected from the cadavers' exteriors of Galleria mellonella larvae. Microscopic observation of these fungal isolates revealed that they belonged to nine fungal genera among them Metarhizium sp. (3 isolates) and Beauveria sp. (5 isolates). Primary experiments showed different bioactivities of the isolates of the two selected genera against the target insect. Only Metarhizium isolates (M3 & M5) and Beauveria isolates (B1 & B5) were selected for further investigations. Identification of fungal cultures by morphological characters, molecular characterization, ITS sequencing, and NCBI Blast showed that two isolates were confirmed as M. anisopliae and two isolates as B. bassiana. The bioassay evaluation revealed that M. anisopliae (M3 and M5) and B. bassiana (B1) exhibited significant virulence against A. craccivora, as evidenced by their high LT50 and LC50 values and substantial sporulation on the cadavers. Therefore, they could be used for the formulation of pesticides targeting this pest on cowpea and other vegetable crops. [ABSTRACT FROM AUTHOR]

Published
2024
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3. Phylogenetic characteristics of novel Bacillus weihenstephanensisand Pseudomonassp. to desert locust, Schistocerca gregariaForskål (Orthoptera: Acrididae)

Author

Mashtoly, Tamer A., El-Zemaity, Mohamed S., Abolmaaty, Assem, Abdelatef, Gamal M., Marzouk, Ashraf A., and Reda, Mohamed

Abstract

Abstract: Thirty bacterial isolates were isolated from the gut contents of diseased/dead locust. Their pathogenicity was tested against 4th instar nymphs of desert locust, Schistocerca gregariaForskål (Orthoptera: Acrididae). Two isolates, designated DL2 and DL6, out of thirty showed the highest insecticidal activities against locust nymphs in preliminary bracketing. They were bioassayed via leaf dip and per os techniques and toxicity was determined using SAS program. The insecticidal activity of DL6 was more than DL2, whereas LC50’s values of 35 × 106and 13 × 106cfu’s/ml were determined for DL2 and DL6, respectively, after 48 h of leaf-dip treatment. However, LD50’s value of 53 × 106and 26 × 106cfu’s/ml was determined for DL2 and DL6, respectively, after 24 h of per os treatment. The relative potencies of DL6 to DL2 were (2.6 and 2.03) folds in leaf-dip and per os treatments, respectively. Biochemical characterization was conducted, using GEN III MicroPlate™ Biolog identification system and confirmed with molecular identification via 16S rDNA gene sequencing. Nucleotide sequencing of each was submitted to a gene bank and an accession number was generated for each isolate. Obtained bacterial strains DL2 and DL6 were identified as Bacillus weihenstephanensis(KY630645) and Pseudomonassp. (KY630649), with a similarity of 100 and 75% to B. weihenstephanensisstrain PHCDB9 (NR_024697) and Pseudomonassp. strain DSM11821 (KF417541), respectively. The tested strains proved their potential to be bio-pesticide agents involved in controlling desert locust nymphs. Graphical abstract:

Published
2019
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