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Copyright of Egyptian Journal of Microbiology is the property of Egyptian National Agricultural Library (ENAL) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

This study to estimate toxigenic diversity of three S. aureus strains (11SaUTI, 5SaRM and 21SaRW) isolated from clinical patient, food and water at Mesallata City, Libya. As well as to know antibiotic resistance, phage typing, biofilm formation and genoypes (icaA gene and 16s rDNA gene). Three S. aureus isolates were selected from isolates obtained from the different sources and identified by conventional biochemical tests and confirmed by VETIk kit. These isolates exhibited variation of toxigenic, antibiotics resistance, biofilm formation and phage plaque forming (phage typing). The amplified 16s rDNA gene to confirm the diversity of S. aureus isolates . In addition to molecular diagnosis to have a complete and clear image for bacterial etiology based on genetic variability. DNA genome of S. aureus isolates was used as template for PCR to amplify 16s rDNA gene. Partially, length of 16s rDNA gene could be synthesized using the internal primer sets combination in PCR to confirm its interspecific to S. aureus with an expected size 1219 bp. The replacement situation of nucleotide sequences were with percent of camera 1.067, 2.217 and 1.313 respectively. Three S. aureus isolates were recorded in gene bank under accession no. LC121093.1 (11SaUTI); LC121094.1 (5SaRM) and LC121095.1 (21SaRW). [ABSTRACT FROM AUTHOR]