10 results on '"El-Mallah AM"'
Search Results
2. In vivo antischistosomal activity profiling and efficacy of niosomal Spirulina platensis and praziquantel combined remedy against murine Schistosoma mansoni infection.
- Author
-
Abdel-Tawab H, Al-Sayed SE, El-Ela FIA, Ibrahim AM, Hammam OA, and El-Mallah AM
- Abstract
Schistosomiasis is a serious parasite disease with a high rate of mortality and negative financial impacts in subtropical and tropical locations like Egypt. The goal of this study was to investigate the anti-schistosomal effect of Spirulina platensis (SP) and Spirulina loaded niosomes (SPN), either in the presence or absence of praziquantel (PZQ) against S. mansoni in experimentally infected mice. Six groups have been involved in the study, five groups were infected with S. mansoni cercariae and subjected independently to different treatments of SP, SPN, and PZQ or the preceding two combinations, in addition to one untreated group which acts as a control. At the 8th week, mice were euthanized, and besides a histopathological assessment of the liver granuloma, the number of worms, tissue egg load, and oogram pattern were estimated. To evaluate the condition of the liver oxidative stress, the levels of malondialdehyde and reduced glutathione in liver homogenates were investigated. Additionally, to assess the anti-inflammatory properties, serum cytokines (IFN-γ, TNF-α, IL-13, and IL-10) and CD4
+ immunohistochemistry expression were determined. The results demonstrated that each of the investigated parameters was significantly changed by both SPN and/or SPN with PZQ treatments alongside PZQ. The highest therapeutic effect was obtained in SPN combined with a half dose of PZQ which achieved 100 % reduction in both the total worm burden and the highest reduction in the intestinal (93.22 %) and hepatic (94.4 %) egg content, as well, moreover 40.5 % reduction of the granuloma size. Furthermore, serum cytokine levels {(TNF-α (11 ± 0.5 (P ˂ 0.001)), (IFN-γ (19.7 ± 1.2 (P ˂ 0.001)) and (IL-13 (53.65 ± 1.4 (P ˂ 0.001))}, as well as CD4+ cells (6.5 ± 0.65 (P ˂ 0.001)) were reduced. While, IL-10 (61.1 ± 2.1 (P ˂ 0.001)) was increased due to the same treatment additional to its antioxidant properties by reduced lipid peroxidation (LPO) (1.1975 ± 0.05(P ˂ 0.001)) but increased reduced glutathione (GSH) (2.31± 0.15 (P˂0.001)). In conclusion, SPN has a schistosomicidal, antioxidant, and hepatoprotective role. SPN has a strong synergistic effect when combined with PZQ which showed anti-inflammatory action. Hence, SPN + PZQ offers promising alternatives for future schistosomiasis therapeutic research., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)- Published
- 2024
- Full Text
- View/download PDF
3. Repellency, Fumigant Toxicity, Antifeedent and Residual Activities of Coridothymus capitatus and Its Main Component Carvacrol against Red Flour Beetle.
- Author
-
Eltalawy HM, El-Fayoumi H, Aboelhadid SM, Al-Quraishy S, El-Mallah AM, Tunali F, Sokmen A, Daferera D, and Abdel-Baki AS
- Subjects
- Animals, Fumigation, Coleoptera drug effects, Cymenes pharmacology, Insect Repellents pharmacology, Insect Repellents chemistry, Oils, Volatile pharmacology, Oils, Volatile chemistry, Insecticides pharmacology, Insecticides chemistry, Tribolium drug effects
- Abstract
Tribolium castaneum is a challenging pest of stored products, causing significant economic losses. The present study explored the efficacy of Coridothymus capitatus essential oil and its primary constituent, carvacrol, as eco-friendly alternatives for managing this pest. To evaluate their insecticidal potential, repellency, fumigant toxicity, and antifeedant properties, progeny inhibition assays were performed. Carvacrol exhibited superior repellency compared to the essential oil, achieving a 92% repellency rate at 2 mg/cm
2 . Both compounds demonstrated significant fumigant toxicity against T. castaneum , with LC50 values of 168.47 and 106.5 μL/L for the essential oil and carvacrol, respectively, after 24 h. Carvacrol also outperformed the essential oil in antifeedant activity, inducing an 80.7% feeding deterrence at 1.17 mg/g. Moreover, both treatments effectively suppressed the development of the pest's progeny. These results collectively underscore the potent insecticidal properties of C. capitatus essential oil and carvacrol, particularly carvacrol, as promising candidates for the sustainable management of T. castaneum in stored product protection.- Published
- 2024
- Full Text
- View/download PDF
4. Synthesis of Carvacrol-Loaded Invasomes Nanoparticles Improved Acaricide Efficacy, Cuticle Invasion and Inhibition of Acetylcholinestrase against Hard Ticks.
- Author
-
Gamal A, Aboelhadid SM, Abo El-Ela FI, Abdel-Baki AS, Ibrahium SM, El-Mallah AM, Al-Quraishy S, Hassan AO, and Gadelhaq SM
- Abstract
Carvacrol is a monoterpenoid phenol found in many essential oils that has antibacterial, antifungal and antiparasitic activities. Drug loaded-invasome systems are used to deliver drugs utilizing nanoparticles to improve bioavailability, efficacy, and drug release duration. As a result, the present study developed carvacrol-loaded invasomes and evaluated their acaricidal effect against Rhipicephalus annulatus (cattle tick) and Rhipicephalus sanguineus (dog tick). Carvacrol loaded-invasome (CLI) was prepared and characterized using UV/Vis spectrophotometer, zeta potential measurements, Scanning Transmission Electron Microscopy (STEM), Fourier Transform Infrared (FT-IR) Spectroscopy, and Differential Scanning Calorimetry Analysis. CLI (5%) induced significant mortality (100%) in R. annulatus adult ticks with LC
50 of 2.60%, whereas the LC50 of pure carvacrol was 4.30%. Carvacrol and CLI were shown to have a significant larvicidal action on both tick species, with LC50 s of 0.24 and 0.21% against R. annulatus and 0.27 and 0.23% against R. sanguineus , respectively. Carvacrol and CLI (5%) induced significant repellent activities for 24 h against R. annulatus and R. sanguineus , as evidenced by the rod method and the petri-dish selective area choice method, respectively. High-performance liquid chromatography (HPLC) demonstrated that the CLI form had 3.86 times the permeability of pure carvacrol. Moreover, carvacrol and CLI inhibited acetylcholinesterase activity and decreased glutathione and malonedealdehyde levels in the treated ticks. In conclusion, invasomes significantly improved adulticidal and repellency activities of carvacrol against both tick species.- Published
- 2023
- Full Text
- View/download PDF
5. Anticoccidial and Antioxidant Activities of Moringa oleifera Leaf Extract on Murine Intestinal Eimeriosis.
- Author
-
Abdel-Tawab H, Abdel-Haleem HM, Abdel-Baki AS, Al-Quraishy S, and El-Mallah AM
- Subjects
- Animals, Antioxidants, Chickens, Mice, Plant Extracts pharmacology, Coccidiosis drug therapy, Coccidiosis veterinary, Eimeria, Moringa oleifera, Poultry Diseases
- Abstract
Background: Coccidiosis is an intestinal disease caused by protozoan parasites of the genus Eimeria and responsible for considerable economic loss in the livestock and poultry industries. Resistance to the current anticoccidial drugs is now a major challenge to efforts to control the disease, and this has stimulated the search for new compounds as alternative treatments. In this context, plant extracts have emerged as an alternative and complementary approach to control coccidiosis. In the present study, an ethanol extract of Moringa oleifera leaves was screened for its anticoccidial activity against Eimeria papillata infection in mice., Methods: To this end, albino mice were allocated into three groups: the first group was the non-infected control; the second and third groups were infected with 10
3 E. papillata oocysts. Of these, the second group was kept as an infected control; while the third group was gavaged with 100 μl of moringa leaf extract (MLE) at a dose of 400 mg MLE/kg, once daily, for five days., Results: MLE significantly suppressed oocyst excretion in faeces, and histological study of the jejunum showed a significant decrease in the number of parasitic stages, with significant improvement in the numbers of goblet cells. Furthermore, the expression of MUC2 gene was upregulated in the treated mice compared with infected, which further supports the anticoccidial potential of MLE. Moreover, our study evidenced that MLE reduced oxidative damage by decreasing TBARS and iNOS expression, and increasing the GSH and GPX levels. Also, treatment with MLE promoted the expression of Bcl-2 and ultimately, inhibited the apoptosis of host cells in the treated mice., Conclusion: Our data indicate that MLE has anticoccidial, anti-oxidant and anti-apoptotic activities in mice infected with Eimeria papillata.- Published
- 2020
- Full Text
- View/download PDF
6. Enhancement of Anti-allergic Effect of Diethylcarbamazine Citrate in Asthmatic Mouse Model: Testing of Anti-drug Antibodies and Quercetin.
- Author
-
Abdul-Razek N, M El-Mallah AM, Abuelsaad ASA, and Abdel-Latif M
- Subjects
- Animals, Antibodies, Monoclonal pharmacology, Asthma diagnosis, Asthma immunology, Asthma metabolism, Biomarkers, Cytokines metabolism, Disease Models, Animal, Drug Synergism, Immunoglobulin E blood, Immunoglobulin E immunology, Mice, Quercetin pharmacology, Treatment Outcome, Anti-Allergic Agents pharmacology, Anti-Asthmatic Agents pharmacology, Asthma drug therapy, Diethylcarbamazine pharmacology
- Abstract
Diethylcarbamazine citrate (DEC) is known as an effective treatment for bronchial asthma because of its ability to reduce eosinophil trafficking to the lung tissue. The current study aimed to potentiate the anti-allergic effect of the drug by passive immunization of the asthmatic model with anti-DEC antibody or prior treatment with quercetin (Qur). Eight mice groups were categorized into control, the model of lung asthma, treated with DEC, passively immunized with anti(α)-bovine serum albumin Ab, anti-DEC Ab, prior exposure to 10, 20, or 40 mg Qur/Kg. b.wt. Both eosinophil peroxidase (EPO) and eotaxin2 in the lung tissues were performed. Serum levels of cytokines, bronchoalveolar lavage fluid (BALF) IgE, rabbit anti-bovine serum albumin (anti-BSA), and DEC IgG in lung tissue homogenates were assayed by ELISA. Regarding the effect of anti-DEC Ab and Qur on DEC-induced recovery of histopathological alterations showed that the Ova group had peri-bronchial hyperplasia, mononuclear leukocyte infiltration, thickening in the wall of alveoli, and congested blood vessels. However, the reduction of inflammatory cells and thickened alveolar walls was dependent on the Qur dose. Qur40 enhanced the anti-allergic effect of DEC. Moreover, the present data revealed high levels of Th2 cytokines (IL-4 and IL-5) and IgE in the Ova group. An increased leukocyte infiltration/thickening of the alveolar wall and lung tissue EPO/eotaxin2 were also observed. Qur-40 could show an enhancement effect on DEC for the reduction of IL-4, IL-5, IgE, EPO, and eotaxin 2. Consequently, the IFN-γ/IL-4 ratio was increased. Qur at 40 mg/Kg could be recommended to enhance the DEC effect suggesting a novel approach for treatment.
- Published
- 2020
- Full Text
- View/download PDF
7. Heat shock protein 70 of filarial parasite Setaria equina: Cloning, expression, and analysis of binding with diethylcarbamazine citrate.
- Author
-
Abdel-Latif M, El-Mallah AM, Bakeer W, and Khalil RG
- Subjects
- Adjuvants, Immunologic chemistry, Adjuvants, Immunologic metabolism, Adjuvants, Immunologic pharmacology, Amino Acid Sequence, Animals, Base Sequence, Cell Proliferation drug effects, Cloning, Molecular, Cross Reactions, Gene Expression, HSP70 Heat-Shock Proteins chemistry, HSP70 Heat-Shock Proteins pharmacology, Helminth Proteins chemistry, Helminth Proteins pharmacology, Humans, Interferon-gamma metabolism, Killer Cells, Natural cytology, Killer Cells, Natural drug effects, Mice, Molecular Docking Simulation, Protein Conformation, Sequence Analysis, Spleen immunology, Diethylcarbamazine metabolism, Filarioidea genetics, HSP70 Heat-Shock Proteins genetics, HSP70 Heat-Shock Proteins metabolism, Helminth Proteins genetics, Helminth Proteins metabolism
- Abstract
Setaria equina heat shock protein (SeqHSP) 70 gene was characterized, cloned and expressed to recombinant protein (rSeqHSP70). The protein was tested for binding with an anti-filarial drug "diethylcarbamazine citrate (DEC)" by equilibrium dialysis method. Molecular docking was also used to determine the binding sites and residues of binding with DEC. The mice were immunized with the protein alone or bound to DEC. Serum IFN-γ levels in the immunized group with protein-drug complex were significantly higher (P < 0.05) than the protein-immunized group. Mouse anti-SeqHSP70 polyclonal IgG recognized 2 bands at 70 and 75 kDa in S. equina adult worm and human cancer cell lines (HepG2 and MCF-7) extracts. The proliferation assay for mice splenocytes revealed a potentiation and down-regulating effects in non-immunized and immunized groups, respectively with the drug-protein complex. The proliferation and IFN-γ assays for purified human NK cells indicated a potentiating effect of the drug-protein complex (DEC concentration is 50 μM) comparable to the protein. DEC at lower concentration (25 mM) could also show a significant increase (P < 0.05) in IFN-γ. From the results, DEC was postulated to induce conformational changes in the protein exposing more epitopes for NK cell binding and activation., (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Published
- 2019
- Full Text
- View/download PDF
8. Antitumoral effect of Ocoxin in hepatocellular carcinoma.
- Author
-
Díaz-Rodríguez E, El-Mallah AM, Sanz E, and Pandiella A
- Abstract
Hepatocellular carcinoma (HCC) is becoming one of the most prevalent types of cancer worldwide. The most efficient types of treatment at present include surgical resection and liver transplantation, but these treatments may only be used in a small percentage of patients. In order to identify novel therapeutic strategies for this disease, the present study explored the potential antitumoral effect of Ocoxin® oral solution (OOS) in HCC. OOS inhibited the proliferation of HCC cell lines in a time- and dose-dependent manner, being more efficient when used in combination with sorafenib, a standard of care treatment for patients diagnosed with advanced-stage disease. Mechanistic studies indicated that the effect of OOS was due to the induction of cell cycle arrest rather than the stimulation of apoptotic cell death. The cell cycle was slowed down in all phases in the HCC cell lines treated with OOS. Finally, when tested in animal models of HCC, OOS reduced tumor progression through the induction of necrosis in xenograft tumor models. Considering the poor prognosis and high resistance to antitumor treatments of HCC, the antiproliferative action of OOS, particularly in combination with sorafenib, provides the opportunity to investigate the effect of combined therapy in a clinical setting.
- Published
- 2017
- Full Text
- View/download PDF
9. Immunomodulatory effect of diethylcarbamazine citrate plus filarial excretory-secretory product on rat hepatocarcinogenesis.
- Author
-
Abdel-Latif M, Sakran T, El-Shahawi G, El-Fayoumi H, and El-Mallah AM
- Subjects
- Animals, Carcinoma, Hepatocellular chemically induced, Cells, Cultured, Diethylnitrosamine administration & dosage, Female, Hepatocytes pathology, Immunoglobulin G blood, Immunomodulation, Interferon-gamma metabolism, Killer Cells, Natural immunology, Leukocytes, Mononuclear immunology, Male, Nitric Oxide Synthase Type II metabolism, Proto-Oncogene Proteins c-bcl-2 metabolism, Rats, Rats, Inbred Strains, Tumor Suppressor Protein p53 metabolism, Antigens, Helminth administration & dosage, Carcinoma, Hepatocellular drug therapy, Diethylcarbamazine administration & dosage, Hepatocytes drug effects, Killer Cells, Natural drug effects, Leukocytes, Mononuclear drug effects, Setaria Nematode immunology
- Abstract
Diethylcarbamazine citrate (DEC) had a significance in anti-filarial chemotherapy, while excretory-secretory product (ES) is released from adult filarial females. The target of the current study was to examine the immunomodulatory effect of DEC, Setaria equina ES or a combination of them on rat hepatocellular carcinoma (HCC) induced by diethylnitrosamine (DEN). In vitro effect of combined DEC and ES or ES alone on lipopolysaccharide (LPS)-stimulated rat peripheral blood mononuclear cells (PBMCs) was tested through IFN-γ assay in culture supernatants. In addition, single or repeated doses of DEC, ES or DEC+ES have been applied in white albino rats to test the effect on HCC. Levels of IFN-γ and anti-ES IgG antibodies in rat serum were assayed using ELISA. Hemolytic complement activity (CH50) was determined in serum while the concentration of nitric oxide (NO) was assayed in liver tissue. The infiltration of NK cells as well as the expression of MHC Iproliferating cell nuclear antigen (PCNA), inducible NO synthase (iNOS), Bcl2 and p53 were determined using immunohistochemistry. There was a dose-dependent increase in IFN-γ after in vitro exposure to DEC+ES. Repeated ES doses increased NO concentration (p<0.05) and expression of iNOS but reduced CH50 (p<0.001), while repeated DEC+ES doses could increase anti-ES IgG (p<0.01), IFN-γ level (p<0.05) and NK cell infiltration. The same treatments could also reduce the expression of MHC I expression, PCNA, Bcl2 and p53. This study has shown immunomodulatory and protective effects of DEC+ES repeated doses on rat HCC., (Copyright © 2014 Elsevier B.V. All rights reserved.)
- Published
- 2015
- Full Text
- View/download PDF
10. Effect of diethylcarbamazine citrate and Setaria equina excretory-secretory material on rat hepatocellular carcinoma.
- Author
-
Abdel-Latif M, Sakran T, El-Shahawi G, El-Fayoumi H, and El-Mallah AM
- Subjects
- Alanine Transaminase blood, Animals, Antioxidants administration & dosage, Antioxidants therapeutic use, Aspartate Aminotransferases blood, Diethylcarbamazine administration & dosage, Female, Lipid Peroxidation drug effects, Liver drug effects, Liver metabolism, Liver pathology, Liver Neoplasms, Experimental metabolism, Liver Neoplasms, Experimental pathology, NF-kappa B metabolism, Oxidative Stress drug effects, Rats, Diethylcarbamazine therapeutic use, Liver Neoplasms, Experimental drug therapy, Setaria Nematode chemistry
- Abstract
Diethylcarbamazine citrate (DEC) has been known for its efficacy to eradicate bancroftian filariasis in Egypt and other countries in the world. One of the known effects was to decrease the level of circulating filarial antigen in the patient's serum. The target of this study was to examine the effect of DEC, excretory-secretory (ES) material from the filarial parasite Setaria equina or a combination of both on the status of oxidative stress and pathogenesis of rat hepatocellular carcinoma (HCC) induced by diethylnitrosamine and 2-acetylaminofluorene. This could be tested in vitro using nitroblue tetrazolium reduction test for measuring the level of superoxide anion (O₂(•-)) released from rat peritoneal macrophages. For in vivo test, a single dose before induction of carcinogenesis or continually repeated doses with DEC, ES or DEC + ES was used. Exposure of macrophages to ES could lead to a significant decrease (p < 0.01) in O₂(•-) release, while DEC (200 μM) could modulate such effect with significant increase (p < 0.05). Pathogenesis of liver cancer and treatment were evaluated using histological investigation, level of antioxidant and liver function enzymes. Repeated ES doses could increase the activity of antioxidant enzymes, especially the catalase enzyme and show a protective effect on liver architecture. DEC could modulate the later effects when combined with ES. No significant effect on the liver function enzymes after treatment was observed. Nuclear factor κB was found to be localized only in the cytoplasm after single and repeated treatments with ES. This study could indicate the effect of S. equina ES as antioxidant against rat HCC, while DEC could modulate such effect when combined with it.
- Published
- 2014
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.