1. Accurate physiological monitoring using lab-on-a-chip platform for aquatic micro-organisms growth and optimized culture
- Author
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Pierre Temple-Boyer, S. Assié Souleille, Jérôme Launay, Eléna Bedel-Pereira, Ludovic Salvagnac, F. Sekli Belaidi, Isabelle Séguy, Marie-Charline Blatché, Équipe MICrosystèmes d'Analyse (LAAS-MICA), Laboratoire d'analyse et d'architecture des systèmes (LAAS), Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées, Service Techniques et Équipements Appliqués à la Microélectronique (LAAS-TEAM), Service Instrumentation Conception Caractérisation (LAAS-I2C), Équipe Matériaux et Procédés pour la Nanoélectronique (LAAS-MPN), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université Toulouse Capitole (UT Capitole), and Université de Toulouse (UT)
- Subjects
Chlamydomonas reinhardtii ,electrochemical sensor ,02 engineering and technology ,010402 general chemistry ,Photosynthesis ,01 natural sciences ,Acclimatization ,law.invention ,[SPI]Engineering Sciences [physics] ,Nutrient ,Exponential growth ,law ,Materials Chemistry ,Electrical and Electronic Engineering ,[SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics ,Instrumentation ,Pollutant ,biology ,Chemistry ,Metals and Alloys ,Oxygen evolution ,algal metabolisms ,Lab-on-a-chip ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,biology.organism_classification ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,lab-on-chip ,13. Climate action ,Environmental chemistry ,green microalgae culture ,0210 nano-technology ,Biosensor - Abstract
International audience; The present work was dedicated to the development of a lab-on-chip microsystem for the monitoring of microalgal photosynthetic activity. Thanks to integrated electrochemical microcells, dissolved oxygen O2 concentrations due to photosynthetic microalgal activity were measured in a continuous way and oxygen production rates of microalgae cultures were finally determined in the frame of artificial night/day cycles. Application was performed by studying the physiological metabolisms of the green microalga Chlamydomonas reinhardtii. First, the different growth dynamics of microalgal cultures were characterized, enabling the determination of the induction, "exponential growth", "declining growth rate" and stationary phases. Then, the influences of carbon-based nutrients, such as sodium bicarbonate HCO3Na and methanol CH3OH, as well as of pollutants, such as silver nitrate AgNO3, were studied, evidencing contradictory behaviors according to the competition between nutritional properties, toxicity effects and acclimation phenomena. This paves the way to the development of analysis microsystems for the understanding of microalgal metabolisms as well as for the improvement of microalgae growth processes and associated industrial production.
- Published
- 2020