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Simple Summary: Laying hens possess a considerable capacity for digestion, yet certain foods contain compounds that hinder the function of digestive enzymes in avian species. These compounds, referred to as antinutritional factors, include phytate. Phytate degradation is facilitated by phytase. Through dosing, the complete elimination of phytate can be achieved, thereby enabling birds to optimize their utilization of food and nutrients previously associated with undigested compost. Consequently, the determination of the optimal phytase dosage for phytate degradation in diets for laying hens during the egg production phase assumes paramount importance. Therefore, the objective of this study was to evaluate this aspect, taking into consideration various physiological and productive efficiency metrics related to laying hens. Despite the acknowledged advancements in the utilization of phytase dosing, limited research has been conducted on commercial laying hens. To assess the effectiveness of doses of exogenous phytase, a total of 320 laying hens aged between 44 and 64 weeks were utilized. These hens were divided into five treatments, which were then distributed using a completely randomized design with eight replicates, each containing eight birds. The treatments consisted of phytase supplementation levels of 0, 500, 1000, 1500, and 3000 FTU/kg. Quadratic regression equations were utilized to determine the maximum and minimum values for the levels of phytase supplementation. A significant difference was observed in the levels of phytase supplementation, where a quadratic model was fitted to various variables including egg weight (g), egg mass (g/bird/day), feed conversion per egg mass (g/g), yolk concentration (%), albumen concentration (%), shell thickness (μm), shell strength (kgf), specific gravity, and yolk color. Further, an increased supplementation improved the digestive tract of the hens, thereby providing a better villus–crypt ratio. Considering the advantageous effects of phytase dosing, it is recommended to supplement corn and soybean meal-based diets with 1500 FTU/kg of bacterial phytase for laying hens during the 44- to 64-week production period. [ABSTRACT FROM AUTHOR]

Background: Turmeric (Curcuma longa L.) is a widely recognized spice and medicinal plant that has gained significant attention for its potential health benefits. This review aims to provide a comprehensive overview of the beneficial applications of turmeric in improving health and egg production in layers. Objective: The objective of this review is to assess the current scientific literature on the effects of turmeric supplementation in layer diets and evaluate its impact on layer health and egg production. Methods: A systematic search was conducted in Google Scholar database to identify relevant studies published in peer‐reviewed journals. Studies investigating the effects of turmeric or its bioactive compound curcumin on layer health and egg production were included. Data on various parameters, including immune function, reproductive performance, egg quality and production parameters, were extracted and analysed. Results: Turmeric contains a bioactive compound called curcumin, which possesses antioxidant, anti‐inflammatory, antimicrobial and immunomodulatory effects. These properties have been extensively studied and have shown promising results in enhancing layer health and performance. Turmeric supplementation has been reported to improve the overall immune response in layers, reducing the incidence and severity of infectious diseases. It has also been shown to have positive effects on gut health by modulating the gut microbiota composition, improving nutrient absorption and reducing digestive disorders. Furthermore, studies have demonstrated that turmeric supplementation in layer diets can improve egg weight, shell quality, yolk colour and egg production rates. The mechanisms underlying these effects involve the antioxidant properties of turmeric, which protect the reproductive organs, enhance ovarian function and improve reproductive performance. Conclusion: The findings underscore the potential of turmeric as a natural, cost‐effective and sustainable intervention for improving layer well‐being, egg quality and productivity. However, further research is needed to fully understand the mechanisms of action, optimize dosage regimens and evaluate the long‐term effects of turmeric supplementation in layer diets. [ABSTRACT FROM AUTHOR]

This study aimed to evaluate the effects of substituting a crude palm oil (CPO) diet with palm-pressed fibre oil (PPFO) on laying hens’ performance, egg production, carcass characteristic and egg quality. A total of 150 Hisex Brown laying hens were randomly assigned to five treatments: a basal diet containing 4% CPO (T1-control) and basal diets in which CPO was substituted by 25% (T2), 50% (T3), 75% (T4) or 100% (T5) PPFO. These diets were fed to laying hens ad libitum for 16 weeks. Compared to the T1 diet, dietary treatments T2, T3 and T4 had no significant effect (P > 0.05) on the feed intake, body weight gain, feed conversion ratio, egg number, egg production, egg weight and egg mass of the laying hens during the entire experimental period. Nonetheless, a significant (P < 0.05) reduction in feed intake, egg number, egg production and egg mass were observed in hens fed T5 compared to those fed the T1 (control) diet. Except for heart and spleen weights, other carcass characteristics were not affected by treatment (P < 0.05). Skin yellowness and breast meat redness increased significantly (linearly, P < 0.05) with increasing proportions of PPFO. Likewise, yolk colour scores increased in proportion to the dietary level of PPFO (linearly, P < 0.05). These findings suggest that PPFO can be used as a novel, cost-effective lipid source at a level of 3% in layer diets as a substitute for CPO to improve eggs’ quality parameters without any harmful effects on laying performance. [ABSTRACT FROM AUTHOR]

OPN5 is one of the main deep brain photoreceptors (DBPs), converting photoperiodic information into neuroendocrine signals to regulate reproduction in birds. This study investigated the mechanism of OPN5-mediated photoperiodic regulation of reproduction by active immunization against OPN5. 96 female quail were divided into OPN5-immunized and control group under the same photoperiod: 16 L:8 D (d 1 to d 35), 8 L:16 D (d 36 to d 70) and 12 L:12 D (d 71 to d 126). OPN5-immunized group was conducted with OPN5 protein vaccination and control group was given a blank vaccine. Samples were collected on d 1, d 30, d 60, and d 126. Results showed switching photoperiod to 8 L:16 D decreased the laying rate, GSI%, numbers of YFs and WFs, serum levels of PRL, P4 and E2, and pituitary PRL and TSHβ protein expressions in both groups (P < 0.05). Whereas the OPN5-immunized group exhibited higher laying rates than the control group (P < 0.05). The control group showed reduced GnRHR and TSHβ gene expressions in the pituitary and increased GnIH and DIO3 transcript and/or protein abundance in the hypothalamus. (P < 0.05). The OPN5-immunized group had lower DIO3 expression at both mRNA and protein levels. (P < 0.05). Switching photoperiod from 8 L:16 D to 12 L:12 D increased the laying rates, GSI%, numbers of YFs and WFs, serum levels of PRL, and PRL protein expression in both groups (P < 0.05), and the responses were more pronounced in OPN5-immunized group (P < 0.05). In contrast to the control group, quail with OPN5-immunization had higher OPN5 and DIO2 transcript and/or protein levels but lower DIO3 expressions in the hypothalamus along the transition photoperiods (P < 0.05). The results revealed that OPN5 responds to photoperiod transition, and its activation mediates related signaling to up-regulate TSH-DIO2/DIO3 pathway and VIP-PRL secretion to prime quail reproductive functions. • OPN5 immunization slowed down the Inhibition of short-photoperiod on reproduction. • OPN5 immunization enhanced the stimulation of prolong-photoperiod on reproduction. • OPN5 immunization enhanced OPN5 signaling to promote follicular development in quail. • OPN5 may have a regulatory effect on PRL while mediating photoperiod. [ABSTRACT FROM AUTHOR]

Simple Summary: The body weight of pullets (i.e., sexually immature chickens) immediately before they start to lay eggs can impact their subsequent egg production, feed intake, and feed efficiency. Therefore, pullets of a particular size may be required to meet performance targets throughout their egg-laying phase. This study evaluated lighting (hours of light/day) and feeding programs during rearing as tools to grow pullets to a designated size. Their performance was then monitored throughout their early egg-laying period. Compared to restricted feeding and shorter lighting/day, ad libitum (ad lib) feeding—or free access to feed—together with longer hours of light/day resulted in higher feed intake and heavier pullets. However, pullets fed ad lib but with fewer hours of light were the first to start to lay eggs. Interestingly, pullets reared with ad lib feeding continued to consume more feed throughout their early egg-laying period. They also produced more eggs, which were heavier than the eggs from hens that, as pullets, had been restricted to lower feed intake during rearing. Hence, lighting and feeding regimens during rearing are practical tools that can achieve specific pullet weight, feed intake, hen weight, egg production, and egg size throughout the early egg-laying period of commercial chickens. As hen body weight (BW) impacts egg weight (EW) and feed efficiency, egg producers prefer pullets of a specific size to enter the egg-laying cycle. Lighting and feeding programs were tested to achieve target Hy-Line Brown pullet BW. Three feeding programs were implemented: ad libitum (ad lib); feeding to achieve breed standard weight for age (BSW); and feeding to achieve 88% BSW (managed). The feeding programs were used with either control lighting (CL: 10 h light/d from 7 weeks of age (WOA)) or reduced lighting (RL: 9 h light/d from 4 WOA). One-hundred and fifty pullets were assigned to each feeding program by lighting treatment during rearing. At 16 WOA, 70 pullets from each treatment during rearing were moved to cages and onto ad libitum feeding under a step-up photoperiod reaching 16 h light/d at 33 WOA. The age and weight of the first egg, hen BW, feed intake (FI), egg production (EP), and EW were measured until 36 WOA. At 16 WOA, pullets reared with ad lib feeding under CL had higher BW and cumulative FI (CFI) compared to ad lib feeding under RL. The latter were the earliest to lay, and the managed pullets under CL were the last to lay. Control lighting and BSW independently generated the heaviest first eggs. At 36 WOA, BW, EW, CFI, and cumulative egg production (CEP) were highest following ad lib feeding during rearing, while rearing under CL generated higher BW and EW but lower CEP than RL. Hence, lighting and feeding programs throughout rearing can regulate pullet growth, FI, and hen performance throughout early lay. [ABSTRACT FROM AUTHOR]

Context: Moringa oleifera leaves present enormous potential as an alternative feedstuff for laying hens. The utilisation of the leaves can be improved by exogenous enzymes owing to the presence of non-starch polysaccharides and phytate. Aims: This study evaluated the influence of Moringa oleifera leaf meal (MOL) in diets supplemented with xylanase and phytase on laying hen performance and egg quality at peak production. Methods: In total, 288 Dekalb White laying hens (32 weeks old) were distributed in a completely randomised design, following a 2 × 4 factorial arrangement (with or without MOL × 4 enzyme supplementations). Each treatment comprised six repetitions, each containing six hens. The control diet was based on ground corn and soybean meal, and the treatment diets were supplemented with 5% MOL, followed by three enzyme additives (xylanase, phytase and a xylanase + phytase blend). Key results: The enzyme supplementation in MOL diets positively influenced egg production (P = 0.012), egg weight (P = 0.021) and egg mass (P = 0.009). The birds offered xylanase-supplemented diets had higher feed intakes (P = 0.015) than did those fed other diets. Moringa oleifera increased the albumen height (P < 0.001) and Haugh unit (P < 0.001), whereas MOL and phytase improved eggshell thickness (P < 0.001) and the candling score (P = 0.049), compared with treatments without phytase supplementation. MOL and enzyme supplementation improved yolk colour intensity (L, A and R parameters) and decreased plasma total cholesterol and LDL cholesterol concentrations. The inclusion of 5% MOL in diets did not impair laying performance. However, when MOL diets were supplemented with exogenous enzymes, there was an increase in egg weight. Conclusions: MOL can be included at 5% in layer diets without affecting performance. When used in combination with xylanase and phytase, they improve performance, intensify yolk colour, and improve shell thickness, candling score, as well as serum biochemical profile. Implications: These findings suggest that the combination of dietary MOL + xylanase + phytase can improve performance and egg quality in laying hens at peak production. The use of alternative foods, as well as additives that improve the use of these foods for birds, is an area of study that is constantly growing owing to the constant search for ingredients that make feed cheaper. Several studies have already used Moringa oleifera as an alternative food in poultry diets; however, studies that combine this food with exogenous enzymes, additives that can improve its use and bring benefits to the animals' health, are still almost non-existent. [ABSTRACT FROM AUTHOR]

1. In recent years, public concern regarding animal welfare has increased while, at the same time, cage systems for animal production have been greatly criticised by EU citizens. In addition, large food retailers promote food products that are made with eggs produced in cage-free systems. 2. The objective of this study was to evaluate the economic viability of the transition of laying hens' to production systems; from conventional to alternative systems with improved welfare. Three independent scenarios were assumed as follows: transition from enriched colony cages to barn (S1), transition from barn to free-range (S2), and transition from free-range to organic (S3). Economic assessments of each transition was applied to a model farm in Greece with 12 000 hens, through partial budget analysis and net benefits and costs were estimated. 3. The results showed a positive economic impact in all transitions to a production system of improved animal welfare (€12,044 in S1, €18,957 in S2 and €7,977 in S3) which indicated that they are economically sustainable. In all scenarios, unit cost increased by 19% in S1, 12% in S2, and 85% in S3. 4. In conclusion, transitioning towards improved animal welfare production systems in laying hen farms could be an economically viable option for egg producers in compliance with societal demands and market trends. [ABSTRACT FROM AUTHOR]

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This study aimed to determine the effect of mineral mix-enriched essential oil (MMEO) on egg production, antibody titer, egg quality, and yolk fatty acid of laying hens. A total of 1680 Lohman Brown laying hens, aged 31 weeks, were randomly divided into two dietary treatment groups. Each group consisted of 6 replicates, with 140 hens per treatment. The hens were fed either a basal diet (CON) or a diet supplemented with 0.25% mineral mix-enriched essential oil (MMEO) for a period of 6 weeks. Egg production and feed efficiency were calculated on every week. On days 21 and 42 of treatments, two eggs per replicate were randomly collected to determine the physical and chemical quality. The antioxidant activity and fatty acid profile of one egg per replicate were analysed at the end of week 6. The results showed that dietary treatment with MMEO increased egg production and feed efficiency compared to the control group. The inclusion increased yolk weight at week 3. However, MMEO supplementation did not affect the physical quality of eggs at week 6. In addition, dietary MMEO supplementation increased the antioxidant activity of yolk at week 6. On contrast, feeding MMEO did not affect the fatty acid profile of yolk. In conclusion, supplementation of mineral mix-enriched essential oil improved laying hens' productivity and antioxidant activity of yolk. [ABSTRACT FROM AUTHOR]

This study evaluated the efficacy of a multi‐protease on the performance, egg quality and digestibility of laying hen. The study had four treatments: Control (without multi‐protease, CON), Pro 1.2 (CON + 0.1% multi‐protease), Pro 2.4 (CON + 0.2% multi‐protease) and Pro 3.6 (CON + 0.3% multi‐protease). Each treatment was replicated six times (replicate = experimental unit = one pen with 15 hens) to give a total of 360 layer hens of the Hy‐line breed. The study lasted for a total of 3 months (14 day adaptation period + 84 days experimental period). The effects of the additive were assessed on: the performance variables, egg quality and ileal amino acid (AA) digestibility. At the end of the study, dietary supplementation with Pro 2.4 and Pro 3.6 improved (p < 0.05) hen‐day egg production, egg mass and eggshell thickness compared with CON at the peak phase. Further improvements (p < 0.05) were observed in the digestibility of crude protein and AAs such as isoleucine, lysine, threonine and cysteine at Pro 2.4 and Pro 3.6 protease supplementation levels compared with CON, while arginine and alanine were higher (p < 0.05) at Pro 3.6 compared with CON. No differences were reported for other performances such as body weight, average daily feed intake, average egg weight, feed conversion ratio, eggshell hardness and all the egg qualities measured. Overall, the results from this study showed better efficacy at Pro 2.4 and Pro 3.6 on the performance of laying hen during the peak phase. [ABSTRACT FROM AUTHOR]

Simple Summary: The olive fruit fly, Bactrocera oleae Gmelin (Diptera: Tephritidae), is one of the most harmful olive pests in Mediterranean countries and worldwide, and its control is mainly based on chemical insecticides. Volatile organic compounds (VOCs) emitted from the olive fruit affect the physiology and behavior of the olive fly and could be an effective tool for its control. In this study, we determined the effect of certain olive fruit VOCs on attraction to olives, egg production, and longevity of B. oleae females, under a series of temperatures. The results show that the exposure of female flies to certain VOCs either increased or decreased the number of landings on the olives, egg production, and longevity. These findings are poised to advance our understanding of the role of olive fruit volatiles on olive fly behavior and may contribute to the improvement of bait or repellent products for its control. Females of the olive fruit fly Bactrocera oleae use various contact and volatile plant stimuli to find olive fruits and lay their eggs on them. We detected certain volatile organic compounds (VOCs) emitted from the olive fruit and studied their effect on female landings on olive fruits, egg production, and longevity under a series of different temperatures from 15 °C to 35 °C. When female flies were maintained at temperatures from 17 °C to 30 °C and exposed to different fruit VOCs either increased or decreased, depending on the substance tested, their landings on olives, egg production, and longevity. Temperature significantly affected the females' responses to fruit VOCs. The highest responses of the flies to fruit VOCs were observed at 30 °C, except for longevity. By contrast, at 15 °C or 35 °C, the flies did not show any response to VOCs. Our results may contribute to a better understanding of the olive fly positive or negative responses to fruit VOCs and the improvement of its control. [ABSTRACT FROM AUTHOR]

Background: Trace elements, such as zinc, magnesium and copper, are essential for improving the performance and health of broiler breeders and the development of chicken embryos. These elements are integral to various proteins involved in metabolism, hormone secretion and the immune system, necessitating their inclusion in small amounts in poultry diets. Objectives: This study aimed to determine the optimal level and effect of different zinc sources on performance, egg quality and the immune system of laying hens at the end of the production period. Methods: The experiment involved 520 Lohmann LSL laying hens, aged 80 weeks, divided into 13 treatments with 5 replications and 8 birds per replication. The hens were fed diets supplemented with 40, 60 and 80 mg/kg of zinc from various sources: mineral zinc oxide, mineral zinc sulphate, organic zinc chelated with glycine and organic zinc chelated with an organic acid. Key parameters measured included body weight, egg weight and immune response. Results: The basal diet contained 63.58 mg/kg of zinc, with the requirement per the Lohmann LSL guideline being 80 mg/kg. Zinc supplementation significantly increased body weight in the second month, with 80 mg/kg being the optimal dose. Zinc oxide notably increased egg weight compared to the control. The hens utilized zinc from all sources, resulting in weight gain and improved parameters such as egg quality. Immune parameters were also positively influenced by zinc supplementation. Conclusions: Zinc supplementation at appropriate levels enhances the performance and egg quality of laying hens, particularly at the end of the production period. It improves bioavailability, enriches eggs and mitigates age‐related declines in productivity. [ABSTRACT FROM AUTHOR]

The present study investigated the biochemical polymorphism of hemoglobin (Hb) and its relationship with performance traits of Ethiopian indigenous and Sasso chicken breeds. A total of 284 chickens reared in three agro-ecologies were examined for genetic diversity and associations with productive traits at Hb locus using agarose gel electrophoresis. The results showed that the HbA allele was dominant in both breeds, and a higher proportion of male chickens were HbAA genotypes, while females were predominantly HbBB types. In the highland agro-ecology, chickens with the HbAA genotype were the most dominant, whereas in mid- and low-land agro-ecologies, chickens with HbBB and HbAB genotypes were found to be more frequent. A moderate level of expected heterozygosity was obtained with 0.47 and 0.445 for indigenous and Sasso chickens, respectively, with an average effective number of alleles per locus of 1.89 and 1.80. Moreover, chickens with HbAA genotypes showed significantly (p ≤ 0.05) higher body weight and linear body measurements than those of HbAB and HbBB genotypes. However, for appendage body structures (comb and wattle dimensions), chickens with the HbAB and HbBB genotypes had higher mean values. Additionally, clutch size (14.2 ± 0.4), clutch length (21.8 ± 0.7), and eight-month egg production (84.1 ± 1.2) were significantly (p ≤ 0.05) higher for hens with HbBB genotypes, followed by those with HbAB-types. Therefore, the considerable hemoglobin variability and significant associations of Hb variants with the performance traits can be sought as guiding information for further genetic improvement interventions in the chicken breeds under investigation. Further microsatellite marker-based genotyping is recommended to validate the higher morphometric values for HbAA genotypes and the better egg production for HbBB and HbAB genotypes. [ABSTRACT FROM AUTHOR]

In this study, the effects of adding dried wild leek and garlic leaves to the diet of laying quails on egg quality, production performance, intestinal histomorphology, nutrient digestibility, and serum biochemical traits were evaluated. 168- 30 weeks aged- laying quails were randomly arranged into 7 treatments (control group and three different levels (0.5%, 1%, and 1.5%) of wild leek or garlic leaves), 6 replicates, and 4 quails per pen. According to the study, the highest hen-day production was achieved by adding 0.5% garlic leaves, and the egg weight was higher with 1% garlic leaves and 0.5% wild leek compared to the control group (P < 0.05). In addition, adding garlic leaves decreased the average daily feed intake and increased high-density lipoprotein concentration. However, adding both herbs did not influence yolk height, albumin height, shell thickness, and Haugh unit (P > 0.05). All supplemented treatments significantly increased the formerly Roche Yolk Color Fan (DSM) index compared to the control. Quails fed with 1% garlic leaves declined serum cholesterol, very low-density lipoprotein, low-density lipoprotein, and uric acid. Moreover, three levels of garlic leaves decreased triglyceride concentration. Adding 0.5% and 1% garlic leaves to the diet could increase the jejunal, and ileal villus height-to-crypt depth ratio. Furthermore, 1% and 1.5% garlic leaves supplementation elevated organic matter, ash, and crude protein digestibility. In conclusion, supplementing 1% garlic leaves to the quail's diet could improve egg weight, intestinal morphology, and nutrient digestibility, improving lipid profiles and reducing uric acid concentration in the serum. However, both plants were rich pigment sources, increasing the DSM index. [ABSTRACT FROM AUTHOR]

SUMMARY: Few studies have modelled the nutrient requirements of breeding ostriches. Results obtained from historical studies were used here to determine the energy, protein and amino acid requirements of breeding ostriches for maintenance and egg production. Two energy scales were used to determine the energy requirement for egg production by ostrich breeders. The efficiency of metabolisable energy (ME) utilisation for energy deposition in the egg (ko) was calculated as 0.8, and the ME required for egg production (MEe) was 12.2 MJ/egg. The effective energy (EE) requirement for egg production (EEe) and maintenance (EEm) was calculated as 7.8 and 13.8 MJ/bird d, respectively. Average total daily balanced protein requirement (TPt) was calculated as 160 g/bird d. Amino acid requirements for maintenance and egg production are given together with recommendations for the daily supply of each amino acid. [ABSTRACT FROM AUTHOR]

Antinutritional factors in feedstuffs may limit their utilization in livestock production, but fermentation process can be used to improve feed quality; however, studies on fermented soybeans for laying hens remain limited. We investigated the effect of fermented soybean meal (FSBM) at various inclusion levels as a partial replacement for soybean meal (SBM) on egg production, egg quality, amino acid digestibility, gut morphology and microbiota, antioxidant capacity and immune response of young laying hens. A total of 360 Hy-line Brown laying hens aged 18 weeks were selected and divided into 5 groups of 6 replicates each and 12 birds per replicate. The control group received a basal diet while the trial group received the basal diet with FSBM included at 2.5%, 5.0%, 7.5% and 10.0%, respectively, for 12 weeks. Our findings revealed that the nutritional value of FSBM was higher compared to that of SBM in terms of reduced content of trypsin inhibitors and increased contents of crude protein, amino acids and minerals. FSBM enhanced egg production (P

This study evaluated the phenotypic and genetic parameters of indigenous Naked Neck (NN), exotic Black Australorp (BA), and their crossbred (NNBA) chickens. A total of 270 day-old chicks (30 per group) were raised under identical conditions from day 1 to 48 weeks of age. Growth performance, including body weight, daily and total gain, feed consumption, and feed conversion ratio (FCR), was highest in BA, followed by NNBA, and lowest in NN. Carcass composition also varied significantly, with BA and NNBA having higher dressing percentages (62.98% and 62.68%, respectively) compared to NN (60.60%). BA produced the heaviest eggs (54.0 g), followed by NNBA (50.9 g) and NN (47.93 g). BA also had the best egg quality, hatchability, and FCR (3.15), with NNBA showing intermediate results. NN had the poorest performance in all traits. Age at sexual maturity was earlier in NNBA (161.23 days) compared to NN and BA. The study concluded that NNBA crossbreds outperformed NN in growth, carcass traits, and egg production, indicating their potential for enhancing rural poultry farming.

The objective of this study was to study the effect of probiotic supplementation in diet on egg production, egg quality, intestinal morphology, and bacterial population in caecum of laying hens. A total of 768 Lohmann Brown-Classic laying hens, aged 42 - 54 weeks, were used. The laying hens were divided into 3 groups, each with 8 subgroups of 32 birds each. The experiment was planned to use a completely randomized design method. The 3 experimental groups were classified based on their dietary treatment as follows: 1) Basal diet (without probiotic supplementation), 2) Basal diet with probiotic 0.01 and 0.02 % in 3, respectively. Data was collected for 12 weeks. Adding probiotic in the diet was no significant difference on egg production, egg quality, and bacterial population in caecum. However, probiotic supplementation in the diet increased villi height of the duodenum significantly improved (p < 0.02), villi height per depth crypt of the duodenum significantly improved (p < 0.05), villi height per depth crypt of the jejunum significantly improved (p < 0.01), and villi height per depth crypt of the ileum significantly improved (p < 0.02). Therefore, studies have shown that dietary probiotic supplementation can improved small intestinal morphology are the most important indexes for measuring the digestive and absorption function of the small intestine are more efficient. [ABSTRACT FROM AUTHOR]

SUMMARY: Spirulina platensis (SP) has been identified as a 'functional food' in nutrition research. This review evaluates the potential of SP as a feed ingredient for laying hens, and to determine whether it can be classified as a functional food. We conducted a review study to assess the impact of Spirulina inclusion on the production of laying hens and the quality of eggs. Literatures have been shown improvement in performance and egg production along with immunomodulating, hepatoprotective and antioxidant effects of SP in laying hens. Additionally, it exhibits significant hypocholesterolemic activity and can enhance egg quality and egg yolk pigmentation. The studies explored a range of Spirulina inclusion levels, from 0.1% to 15%. Incorporating 1–3% SP in laying hens' diet can improve productive performance and internal egg quality parameters across published research. However, since the results were conflicting, we conducted a meta-analysis review using statistical data from various studies. This meta-analysis aims to assess the impact of Spirulina inclusion on laying hens production, and yolk colour. The Scopus, PubMed, Web of Science, and Google Scholar databases were searched from 1990 to 2024. The results showed that the use of Spirulina significantly reduced egg weight (standard difference in means (SDM): 0.223; I2: 92.073; 95% CI: −0.429, −0.018; P: 0.033) and yolk colour (SDM: −0.574; I2: 86.987; 95% CI: −0.930, −0.218; P: 0.002) in the control groups compared to the Spirulina-fed birds. However, egg mass (SDM: −0.345; I2: 88.094; 95% CI: −0.692, −0.002; P: 0.051), feed intake (SDM: −0.153; I2: 94.165; 95% CI: −0.475, 0.169; P: 0.352), and feed conversion ratio (FCR) (SDM: 0.106; I2: 86.604; 95% CI: −0.089, −0.302; P: 0.287) were not significantly affected by dietary Spirulina. Therefore, this meta-analysis concludes that Spirulina inclusion, at the doses tested, does not affect the main production performance parameters of laying hens. [ABSTRACT FROM AUTHOR]

According to their respective positions, participants at the local, regional, and global actors within the poultry farming sector play strategic roles according to their respective positions. While these roles may integrate and overlap, they can also generate conflicts of interest at both the actor and institutional levels. This study analyses the actors and power relations in laying hen farming in Blitar Regency, East Java, Indonesia. The data were collected using structured interview techniques (interview guides and Matrix of Alliances and Conflicts: Tactics, Objectives and Recommendations questionnaires), observation, documentation, and focus group discussions. The research informants were chosen deliberately based on various sources including farmers, farmer groups, cooperative, central and local government, companies, and traders. This was in addition to informants obtained using the snowball sampling technique such as middlemen, poultry shops (PSs) and other additional breeders. Data validity was determined using source triangulation and method triangulation. The results revealed that the individuals and entities engaged in layer hen farming encompass a diverse range of actors. These include farmers of various scales (small, medium, and large), institutions such as farmer groups and cooperatives, governmental bodies at both central and regional levels, industry representatives such as companies, PSs, distributors involved in producing and marketing day-old chick (DOC), feed, medicines, and vaccines, as well as market participants including local traders and traders operating across different regions. Small-scale and medium-scale farmers have the strongest relationships due to their high dependence on inputs such as DOC, feed, vaccines, and access to markets. The presence of various actors and power relations in laying hen business institutions creates both collaboration and competition in the supply of DOCs, animal feed, medicine, and even in the marketing of eggs from a food security and sustainability perspective.

Abstract Environmental conditions profoundly impact the health, welfare, and productivity of laying hens in commercial poultry farming. We investigated the association between microclimate variations, production indices, and histopathological responses to accidental Newcastle disease virus (NDV) infection within a controlled closed-house system. The study was conducted over seven months in a laying hen facility in Cairo, Egypt. Microclimate measurements included temperature, relative humidity (RH%), air velocity (AV), and the temperature humidity index (THI) that were obtained from specific locations on the front and back sides of the facility. Productivity indices, including the egg production percentage (EPP), egg weight (EW), average daily feed intake, and feed conversion ratio, were assessed monthly. During an NDV outbreak, humoral immune responses, gross pathology, and histopathological changes were evaluated. The results demonstrated significant (p

The study was conducted at the Aleppo University Research Centre. It evaluated the effects of a genetic improvement programme of inbreeding and epigenetics by adding different levels of turmeric powder in diets on production traits. It also used epigenetic and phenotypic information to reduce the negative effects of fourth-generation female inbred Syrian quail genotypes, estimate production/genetic parameters on egg production/quality traits, and establish phenotypic and genetic correlations between parent-offsprings through variance components in the generations. Data analysis of the fourth generation indicated that the second group 1% turmeric recorded the highest value for most of the egg production coefficients. Heritability coefficient values for the studied traits ranged from 0.22 to 0.68 while phenotypic correlations for EN and EM traits were positive 0.88. Strong genetic correlations were observed between ShW and YW, and EM and YW, respectively. Multigenerational genetic improvement programmes using inbreeding to create pure breeds and epigenetics using turmeric to reduce the negative effects of inbreeding and improve production parameters for local Syrian quail.

This experiment was conducted to estimate the main effect of adding Data palm pollen (DPP) and the form of diet on the productive performance of female quail compliant with: Hen day egg production (HD), Egg weight (EW), Egg mass (EM), Feed intake (FI) and Feed conversion ratio (FCR) and eggs quality of quail compliant with: Shape index (ShI %), shell weight (%), yolk weight (%), albumen weight (%), Yolk index (YI%), Albumen Index (AI%) and Hough unit (HU). For eight weeks, from February 14 to April 13, 2023. Two hindered fifty-two female quail with age about forty-seven days were divided into six treatments, each including forty-two birds with three replicates (four ten quail of each). The treatments were T1: 0% DPP with mash feed form, T2: 0% DPP with pellet feed form, T3: 0.5% DPP with mash feed form, T4: 0.5% DPP with pellet feed form, T5: 1% DPP with mash feed form and T6: 1% DPP with pellet feed form. The quail were fed a productive diet with 2794 kcal/kg energy and 18.39% crude protein, and similar rearing conditions. Results showed there were significant effects in the percentage of HD, EW., EM, and (FCR) in treatments with DPP and pellet feed form when compared with the other treatments, as well as an improvement in egg quality in treatments with DPP and pellet feed form when compared with the other treatments.

The current feeding study was designed to validate the two dietary essential amino acid profiles (EAAP) established based on linear broken-line (LBL) and quadratic broken-line (QBL) models, in a previous study, against Evonik (AMINOHen) and breeder recommendations for ISA Brown layers for peak production (PP, 20 to 44 weeks of age), and post peak production (post PP, 44 to 75 weeks of age). The EAAP based on LBL models on average had 19.5% and 26.0% lower digestible AA (Lys, Met + Cys, Thr, Trp, Ile and Val), than the EAAP based on QBL models for PP and post PP, respectively. The EAAP based on AMINOHen and breeder recommendation had lower digestible AA than QBL, and higher EAAP than LBL models for both production phases. At 20 weeks of age, 224 ISA Brown layer hens were weighed and randomly allocated to individual battery cages. Each of the four diets was replicated 8 times with 7 birds per replicate. Egg production was recorded daily, and egg weights were measured at the end of each week. Feed consumption was measured at the end of each period. The egg production rate was not significantly affected by the diets and remained at around 98.0% (PP) and 95.0% (post PP) (P > 0.05). Birds fed diets based on LBL recommendation consistently laid smaller eggs, resulting in a lower egg mass (59.8 vs. 62.0 g egg/hen per day during PP, and 60.3 vs. 63.0 g egg/hen per day during post PP; P 0.05). The highest feed conversion ratio (FCR) during PP (P = 0.067) and post PP (P

Introduction: Laying performance is a key factor affecting production efficiency in poultry, but its molecular mechanism is still indistinct. In this study, Yaoshan chickens, a local breed in Guizhou, China, and merchant chickens (GYR) with higher egg yield after the three-line cross improvement hybridization of Yaoshan chickens were used as animal samples. Methods: To explore the regulatory mechanism of the diversities in laying performance, RNA-seq and ultra-performance liquid chromatographytandem mass spectrometry (UPLC--MS/MS) were used to describe the transcriptional and metabolic profiles of the ovaries of Yaoshan and GYR chickens. Results: At the transcriptional level, 288 differentially expressed genes were upregulated in Yaoshan chickens and 353 differentially expressed genes were upregulated in GYR chickens. In addition, GSEA showed that ECM-receptor interactions and the TGF-β signaling pathway were restrained, resulting in increased egg production in GYR chickens. Furthermore, the upregulation of thiamine and carnitine was identified by metabolomic analysis to facilitate the laying performance of hens. Finally, comprehensive analyses of the transcriptome and metabolome found that thiamine and carnitine were negatively correlated with ECM-receptor interactions and the TGF-β signaling pathway, which jointly regulate the laying performance of Yaoshan chickens and GYR chickens. Discussion: Taken together, our research delineates differences in the transcriptional and metabolic profiles of the ovaries of Yaoshan and GYR chickens during the peak egg production period and provides new hypotheses and clues for further research on poultry egg production performance and the improvement of economic benefits. [ABSTRACT FROM AUTHOR]

Environmental conditions profoundly impact the health, welfare, and productivity of laying hens in commercial poultry farming. We investigated the association between microclimate variations, production indices, and histopathological responses to accidental Newcastle disease virus (NDV) infection within a controlled closed-house system. The study was conducted over seven months in a laying hen facility in Cairo, Egypt. Microclimate measurements included temperature, relative humidity (RH%), air velocity (AV), and the temperature humidity index (THI) that were obtained from specific locations on the front and back sides of the facility. Productivity indices, including the egg production percentage (EPP), egg weight (EW), average daily feed intake, and feed conversion ratio, were assessed monthly. During an NDV outbreak, humoral immune responses, gross pathology, and histopathological changes were evaluated. The results demonstrated significant (p < 0.05) variations in EPP and EW between the front and back sides except in April and May. AV had a significant (p = 0.006) positive effect (Beta = 0.346) on EW on the front side. On the back side, AV had a significant (p = 0.001) positive effect (Beta = 0.474) on EW, while it negatively influenced (p = 0.027) EPP (Beta = − 0.281). However, temperature, RH%, and THI had no impact and could not serve as predictors for EPP or EW on either farm side. The humoral immune response to NDV was consistent across microclimates, highlighting the resilience of hens. Histopathological examination revealed characteristic NDV-associated lesions, with no significant differences between the microclimates. This study underscores the significance of optimizing microclimate conditions to enhance laying performance by providing tailored environmental management strategies based on seasonal variations, ensuring consistent airflow, particularly near cooling pads and exhaust fans, and reinforcing the importance of biosecurity measures under field challenges with continuous monitoring and adjustment. [ABSTRACT FROM AUTHOR]

Fasciolosis, a globally re-emerging zoonotic disease, is mostly caused by the parasitic infection with Fasciola hepatica, often known as the liver fluke. This disease has a considerable impact on livestock productivity. This study aimed to evaluate the fluke burdens and faecal egg counts in goats that were administered phage clones of cathepsin L mimotopes and then infected with F. hepatica metacercariae. Additionally, the impact of vaccination on the histology of the reproductive system, specifically related to egg generation in adult parasites, was examined. A total of twenty-four goats, which were raised in sheds, were divided into four groups consisting of six animals each. These groups were randomly assigned. The goats were then subjected to two rounds of vaccination. Each vaccination involved the administration of 1 × 1013 phage particles containing specific mimotopes for cathepsin L2 (group 1: PPIRNGK), cathepsin L1 (group 2: DPWWLKQ), and cathepsin L1 (group 3: SGTFLFS). The immunisations were carried out on weeks 0 and 4, and the Quil A adjuvant was used in combination with the mimotopes. The control group was administered phosphate-buffered saline (PBS) (group 4). At week 6, all groups were orally infected with 200 metacercariae of F. hepatica. At week 22 following the initial immunisation, the subjects were euthanised, and adult F. hepatica specimens were retrieved from the bile ducts and liver tissue, and subsequently quantified. The specimens underwent whole-mount histology for the examination of the reproductive system, including the testis, ovary, vitellaria, Mehlis' gland, and uterus. The mean fluke burdens following the challenge were seen to decrease by 50.4%, 62.2%, and 75.3% (p < 0.05) in goats that received vaccinations containing cathepsin L2 PPIRNGK, cathepsin L1 DPWWLKQ, and cathepsin L1 SGTFLFS, respectively. Animals that received vaccination exhibited a significant reduction in the production of parasite eggs. The levels of IgG1 and IgG2 isotypes in vaccinated goats were significantly higher than in the control group, indicating that protection is associated with the induction of a mixed Th1/Th2 immune response. The administration of cathepsin L to goats exhibits a modest level of efficacy in inducing histological impairment in the reproductive organs of liver flukes, resulting in a reduction in egg output. [ABSTRACT FROM AUTHOR]

Spawning performance -relative fecundity and fertilization success-was studied in two hatchery-reared broodstocks of gilthead seabream (Sparus aurata) held under relatively constant well-water temperature (18-20 °C) and simulated natural photoperiod, for five consecutive spawning seasons, between 3 and 7 years of age. The spawning season lasted between 4 and 6 months each year, and the total number of eggs produced annually ranged between 1 480 000 and 3 100 000 eggs/kg female body weight, being the highest during the first and second spawning season. No difference was observed in monthly relative fecundity between years -although high variation existed within years, whereas fertilization success was the highest during the second and third reproductive season, and dropped significantly during the last year of the study. The male percentage of the broodstocks was 35% during the first spawning season of the females, and thereafter it decreased further and remained stable at around 15%-20% for the second and third spawning season. Substitution of older females with smaller males to readjust male percentage to 50% in the fourth spawning season, was followed by sex change of larger males to females and a drop of the male percentage to 18% in the following spawning season. The present study demonstrated the long spawning season of gilthead seabream -especially under constant water temperature, the high fecundity and fertilization success, and the stabilization of male percentage to ~20% after the first spawning season or when a broodstock is modified to increase male percentage. These results are useful to the aquaculture industry, demonstrating that there is no need to add males to gilthead seabream broodstocks over the years, as the sex ratio is stabilized and egg production and fertilization success remain high with a relatively low male:female sex ratio. [ABSTRACT FROM AUTHOR]

Reproductive dynamics of commercially important penaeid prawn Penaeus merguiensis was investigated in the coastal waters of Maharashtra during 2002-2003 by collecting data from inshore nearshore and offshore, waters. The size at maturity was 123 mm for males and 133 mm for females. The species spawned throughout the year with two spawning peaks, major one during March-May and minor during October-December and spawning area of the species is offshore waters >40 m depth. Ova diameter study showed tendency to shed all their eggs at one instance, but the progression of modal sizes of mature females demonstrated that females with spawning periodicity of 26±5.5 days could spawn about 12 times during their life span. The sex ratio showed that mainly large sized mature females migrate towards offshore deeper waters. Fecundity was exponentially related to length by F= 0.000000528*TL5.2086. The population fecundity index in nearshore and offshore areas indicated two peaks with major spawning activity during March-April and minor during August-September. The egg production by the surviving population in the sea, gave major peak during April-May and a minor during November. The occurrence of juveniles in the catch was bimodal and their mean size declined twice in inshore, nearshore and offshore waters which pointed to two recruitment pulses in March and November in inshore waters and in March and December in nearshore and offshore waters. The percentage of juveniles was the highest in March which suggested that post-monsoon spawning in November was the most effective. The ratio of juveniles and the egg production gave annual survival rate of 0.03%. The monthly survival showed bimodal trend with peak in April (0.07%) and November (0.03%), which suggested that spawning during November-December and May-June was responsible for the two cohorts, the post-monsoon and pre-monsoon, among which the former was more successful. [ABSTRACT FROM AUTHOR]

There are inconsistent results on the effect of dietary lactobacillus supplementation on laying performance. Therefore, this study ascertained the effect of lactobacillus supplementation on laying hen performance. A total of 348 publications were identified from a methodical search done in five databases, of which 12 met the eligibility conditions for the meta-analysis. Eligibility criteria were information on feed intake (FI), feed conversion ratio (FCR), egg production (EP), egg weight (EW), egg mass (EM), Haugh unit (HU), eggshell thickness (EST), egg yolk, blood cholesterol, and moderators (i.e., treatment duration, Lactobacillus spp. and supplementation dose, hen age, and strains). Data obtained were analyzed in OpenMEE software, and pooled results showed that FCR, EP, EM, HU, EST, egg yolk, blood and egg yolk cholesterol levels were better in the lactobacillus-supplemented group than the controls, taking significant heterogeneity into consideration. Furthermore, results indicate that studied moderators influenced the results of the meta-analysis and explained the variabilities across studies utilized for the meta-analysis. In conclusion, dietary lactobacillus supplementation improved feed conversion ratio, egg production, aspects of egg quality, and blood cholesterol levels in laying hens and could be added to chicken feed to boost laying performance and egg quality. [ABSTRACT FROM AUTHOR]

Yeast culture (YC) plays a significant role in enhancing the performance and health of poultry breeding. This study investigated the impact of different YC supplementation concentrations (basal diet with 1.0 g/kg and 2.0 g/kg of YC, YC1.0, and YC2.0) on egg production performance, egg quality, antioxidant properties, intestinal mucosal structure, and intestinal flora of laying hens. Both YC1.0 and YC2.0 groups significantly enhanced the egg protein height, Haugh unit, and crude protein content of egg yolks compared to the control group (p < 0.05). The supplementation with YC2.0 notably increased the egg production rate, reduced feed-to-egg ratio, and decreased the broken egg rate compared to the control group (p < 0.05). Additionally, YC supplementation enhanced serum total antioxidant capacity (T-AOC) and glutathione peroxidase (GSH-PX) activity while reducing malondialdehyde (MDA) content (p < 0.05). Moreover, YC supplementation promoted duodenal villus height and villus ratio in the duodenum and jejunum (p < 0.05). Analysis of cecal microorganisms indicated a decrease in Simpson and Shannon indices with YC supplementation (p < 0.05). YC1.0 reduced the abundance of Proteobacteria, while YC2.0 increased the abundance of Bacteroidales (p < 0.05). Overall, supplementation with YC improved egg production, quality, antioxidant capacity, intestinal morphology, and cecal microbial composition in laying hens, with significant benefits observed at the 2.0 g/kg supplementation level. [ABSTRACT FROM AUTHOR]

Egg production plays a pivotal role in the economic viability of hens. To analyze the genetic rules of egg production, a total of 3151 Luhua chickens were selected, the egg production traits including egg weight at first laying (Start-EW), egg weight at 43 weeks (EW-43), egg number at 43 weeks (EN-43), and total egg number (EN-All) were recorded. Then, the effects of related factors on egg production traits were explored, using a multi-trait animal model for genetic parameter estimation and a genome-wide association study (GWAS). The results showed that body weight at first egg (BWFE), body weight at 43 weeks (BW-43), age at first egg (AFE), and seasons had significant effects on the egg production traits. Start-EW and EW-43 had moderate heritability of 0.30 and 0.21, while EN-43 and EN-All had low heritability of 0.13 and 0.16, respectively. Start-EW exhibited a robust positive correlation with EW-43, while Start-EW was negatively correlated with EN-43 and EN-All. Furthermore, gene ontology (GO) results indicated that Annexin A2 (ANXA2) and Frizzled family receptor 7 (FZD7) related to EW-43, Cyclin D1 (CCND1) and A2B adenosine receptor (ADORA2B) related to EN-All, and have been found to be mainly involved in metabolism and growth processes, and deserve more attention and further study. This study contributes to accelerating genetic progress in improving low heritability egg production traits in layers, especially in Luhua chickens. [ABSTRACT FROM AUTHOR]

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The higher availability of zinc (Zn) from organic than inorganic sources is already established, but more assertive and cost-friendly protocols on the total replacement of inorganic with organic Zn sources for laying hens still need to be developed. Because some discrepancy in the effects of this replacement in laying hen diets is noticeable in the literature, the objective of this meta-analysis was to properly quantify the effect size of total replacing inorganic Zn with organic Zn in the diet of laying hens on their laying performance, egg quality, and Zn excretion. A total of 2340 results were retrieved from Pubmed, Scielo, Scopus, WOS, and Science Direct databases. Of these, 18 primary studies met all the eligibility criteria and were included in this meta-analysis. Overall, the replacement of inorganic Zn with organic Zn, regardless of other factors, improved (p < 0.01) egg production by 1.46%, eggshell thickness by 0.01 mm, and eggshell resistance by 0.11 kgf/cm2. Positive results of the same nutritional strategy on egg weight and Zn excretion were only observed at specific conditions, especially when organic Zn was supplemented alone in the feed, not combined with other organic minerals. Therefore, there is evidence in the literature that the total replacement of inorganic Zn with organic Zn improves egg production, eggshell thickness, and eggshell resistance. Factors such as hen age and genetics, organic Zn source, concentration of Zn in the feed, and the strategy of its supplementation have to be more carefully considered in protocols designed to address egg weight and Zn excretion by the hen. [ABSTRACT FROM AUTHOR]

Alternative ingredients for the manufacture of poultry feeds need to be identified to meet the growing demand. A 42-day feeding trial was conducted to investigate the effects of the inclusion of mango peel waste in layer chicken diets on performance and egg quality. This study involved one hundred and fifty Isa Brown layer chickens aged 60 weeks. These chickens were assigned to five treatments with graded levels of mango peels: 0% (Treatment 1), 3.5% (Treatment 2), 7% (Treatment 3), 14% (Treatment 4) and 28% (Treatment 5), using a completely randomized design (CRD). Daily egg production was recorded, and weekly measurements included feed intake, specific gravity, egg weight, shell weight and shell thickness. Notably, Treatment 5 exhibited the highest feed conversion ratio (3.09) and Roche yolk color (RYC) fan score (14.3), which was significantly (p < 0.05) different from Treatment 1, with values of 2.36 and 12.4, respectively. Layer chicken fed on T1 had the highest egg weight and egg thickness (6.6 g and 0.44 mm, respectively), differing significantly (p < 0.05) from Treatment T5 eggs (6.3 g and 0.41 mm). It was concluded that mango peels could substitute maize in layer chicken diets up to 7% without affecting production and egg quality. Mango peels are recommended for partial substitution of maize in layer chicken diets and as natural egg yolk pigment to impart the yellow yolk desired by consumers. [ABSTRACT FROM AUTHOR]

ABSTRACT Background Turmeric (Curcuma longa L.) is a widely recognized spice and medicinal plant that has gained significant attention for its potential health benefits. This review aims to provide a comprehensive overview of the beneficial applications of turmeric in improving health and egg production in layers. Objective The objective of this review is to assess the current scientific literature on the effects of turmeric supplementation in layer diets and evaluate its impact on layer health and egg production. Methods A systematic search was conducted in Google Scholar database to identify relevant studies published in peer‐reviewed journals. Studies investigating the effects of turmeric or its bioactive compound curcumin on layer health and egg production were included. Data on various parameters, including immune function, reproductive performance, egg quality and production parameters, were extracted and analysed. Results Turmeric contains a bioactive compound called curcumin, which possesses antioxidant, anti‐inflammatory, antimicrobial and immunomodulatory effects. These properties have been extensively studied and have shown promising results in enhancing layer health and performance. Turmeric supplementation has been reported to improve the overall immune response in layers, reducing the incidence and severity of infectious diseases. It has also been shown to have positive effects on gut health by modulating the gut microbiota composition, improving nutrient absorption and reducing digestive disorders. Furthermore, studies have demonstrated that turmeric supplementation in layer diets can improve egg weight, shell quality, yolk colour and egg production rates. The mechanisms underlying these effects involve the antioxidant properties of turmeric, which protect the reproductive organs, enhance ovarian function and improve reproductive performance. Conclusion The findings underscore the potential of turmeric as a natural, cost‐effective and sustainable intervention for improving layer well‐being, egg quality and productivity. However, further research is needed to fully understand the mechanisms of action, optimize dosage regimens and evaluate the long‐term effects of turmeric supplementation in layer diets.

Egg performance significantly impacts the development of the local goose industry. The hypothalamus plays an essential role in the egg production of birds. However, few potential candidate genes and biological functions related to egg production in geese have been identified in hypothalamus tissue. In this study, 115 geese were raised and observed for 5 months during the laying period. To understand the regulation mechanism of egg production, the hypothalamus transcriptome profiles of these geese were sequenced using RNA-seq. The hypothalamus samples of four high egg production (HEP) and four low egg production (LEP) geese were selected and collected, respectively. A total of 14,679 genes were identified in the samples. After multiple bioinformatics analyses, Gene Ontology (GO) annotations indicated that genes related to egg production were mainly enriched in biological processes of “response to light stimulus,” “sensory system development,” and “visual perception.” Six potential candidate genes (PDE6C, RHO, MFRP, F2, APOB, and IL6) based on their corresponding GO terms and interaction networks were identified. These identified candidate genes can be used as selection markers to improve the egg production of Wanxi white geese. Our study highlights how visual perception may affect the regulation of geese egg production.

Abstract Background Trace elements, such as zinc, magnesium and copper, are essential for improving the performance and health of broiler breeders and the development of chicken embryos. These elements are integral to various proteins involved in metabolism, hormone secretion and the immune system, necessitating their inclusion in small amounts in poultry diets. Objectives This study aimed to determine the optimal level and effect of different zinc sources on performance, egg quality and the immune system of laying hens at the end of the production period. Methods The experiment involved 520 Lohmann LSL laying hens, aged 80 weeks, divided into 13 treatments with 5 replications and 8 birds per replication. The hens were fed diets supplemented with 40, 60 and 80 mg/kg of zinc from various sources: mineral zinc oxide, mineral zinc sulphate, organic zinc chelated with glycine and organic zinc chelated with an organic acid. Key parameters measured included body weight, egg weight and immune response. Results The basal diet contained 63.58 mg/kg of zinc, with the requirement per the Lohmann LSL guideline being 80 mg/kg. Zinc supplementation significantly increased body weight in the second month, with 80 mg/kg being the optimal dose. Zinc oxide notably increased egg weight compared to the control. The hens utilized zinc from all sources, resulting in weight gain and improved parameters such as egg quality. Immune parameters were also positively influenced by zinc supplementation. Conclusions Zinc supplementation at appropriate levels enhances the performance and egg quality of laying hens, particularly at the end of the production period. It improves bioavailability, enriches eggs and mitigates age‐related declines in productivity.

Agricultural producers are increasingly using plant-based feed additives in the diets of livestock and poultry as a way to increase animal productivity and stabilize the protective functions of the body. The purpose of the research was to study the effectiveness of using Astragalus cicer feed supplement in the diets of laying hens of the Lohmann Brown-Light cross at the age of 25-51 weeks. The composition of the compound feed included a phytoadditive made from Astragalus cicer in the volume: I experimental group – 3.60 %, II experimental – 7.20 % by the weight of the compound feed. The duration of the scientific and production experiment was 183 days. The analysis of the content of biologically active substances in the Astragalus cicer phytoadditive has revealed a high content of dihydroquercetin – 336.25 mg/100g of absolutely dry substance.image As the result of the research, it was found that laying hens of the II experimental group, which received 7.20 % of Astragalus cicer phytoadditive in the compound feed, had more intensive metabolic processes in the body, which contributed to obtaining a greater effect from them. Laying hens of the II experimental group compared with the control and I experimental groups had 2.19 and 2.18 % higher values of nitrogen utilization out of taken feed, egg production was higher by 2.81 and 1.10 %, the cost of compound feed decreased by 10 pcs. eggs, that was by 4.76 and 2.38 %, respectively. The profitability level of egg production in the II experimental group was higher compared with the control and I experimental groups by 3.83 and 5.16%, respectively.

Rearing practices prior to sexual maturity have implications on hen performance. The objective of the current study was to evaluate egg production and quality in Lohmann LSL lite hens reared on low or high spacing allowances (SA) in enriched cages and fed diets with a source of omega-3 fatty acids (n-3 FA) or yeast bioactives (YB) from placement to 17 wk of age (woa). The high and low SA were: 348 cm² /bird; ~50 birds/cage) and 284 cm² /bird; ~68 birds/cage, respectively. The diets provided were control (C), C + 3% co-extruded full fat flaxseed and pulse mixture (FFF; 1:1 wt/wt) for n-3 FA and C + YB, yeast cell walls processed with β-1,3-glucan hydrolase (Tables 1-3). At the beginning of 18 woa, a total of 288 hens were placed in 72 enriched cages (4 hens/cage) based on rearing treatments (n = 12). The hens had ad libitum access to commercial layer diet and water. From 52 to 72 woa, eggs were recorded daily for calculation of hen day egg production (HDEP). Egg weight (EW), shell thickness (EST), shell breaking strength (ESBS), yolk color (YC), and Haugh unit (HU) were analyzed on bi-weekly basis. Egg mass was calculated based on EW and HDEP. Data were subjected to PROC GLIMMIX procedure of SAS with rearing SA, diets, and associated interactions as fixed effects. The cumulative impact of the diets on HDEP did not exhibit significant differences (Table 4). However, the space allowance (SA) demonstrated an effect (P < 0.05) with hens reared in increased SA yielding greater HDEP (98.2 to 94.5%) compared with hens reared in decreased SA (94.0 to 90.8%) at 56, 58, 60, 63 and 71 woa. Furthermore, hens reared in SA exhibited greater HU values (P < 0.05) throughout, although egg weight and mass were not affected (P > 0.05; Table 5-8) by SA. In conclusion, under the conditions of the study, provision of n-3 FA and YB did not present noticeable carryover impact on egg production and quality during the mid to late laying cycle. However, rearing spacing allowance influenced egg production and quality. Suggesting, reducing rearing stress through the provision of higher space allowance prior to sexual maturity had carryover effects on hen productivity in late lay cycle. [ABSTRACT FROM AUTHOR]

The aim of this study was to investigating the effects of partially replacing the commercial soybean meal (SBM) by locally produced raw, full-fat soybean (RFFSB) in diets of layers. After cleaning, the tested ingredient (RFFSB) was hammered to pass through a 0.2-mm sieve. Then, four experimental diets were formulated by replacing the SBM by RFFSB at 0, 15, 30 or 45% (equivalents to 0, 30, 60 or 90 g/kg of diet, respectively). Before the commencement of this feeding trial, birds were uniformly managed and fed as per their requirements (i.e., starter, grower and pullet diets). This feeding trial was started when birds’ age was 24 weeks. Every treatment was replicated 4 times and 17 laying birds per replicate. The results revealed that replacing the commercial SBM by raw soybean (up to 45%) in the layer diets had no negative effects on the final live BWT and also on the vital organ developments, such as pancreas, duodenum, intestines and gizzard. Hen-day egg production, hen-housed egg production and egg quality measuring parameters were not significant affected by that of partially replacing the commercially SBM by the raw soybean. It is concluded that without compromising the productivity and health, a hammered RFFSB can replace (up to 45%) the commercial SBM in diets of the laying hens.

Objectives: This research aimed to examine how yeast (Saccharomyces cerevisiae) fermented layer moist diet (YFM-D) influencing egg production, its quality, and duck's growth performance during the age range of 23–29 weeks. Materials and Methods: A total of 84 laying Jinding ducks were arbitrarily allotted into one of two dietary groups. Each group had 42 birds in 3 replicates and 14 birds per replication. Each duck was daily offered 140 gm dry matter (DM) from a layer diet (Crude Protein −17.83% and ME-2760 Kcal/kg DM) and a yeast-fermented layer moist diet (50% layer diet + 50% water) and reflected as control diet (CON-D) and yeast-fermented layer moist diet (YFM-D), respectively. The layer diet was fermented using yeast premix at a 1.0% level of feed. Results: Considerably elevated DM intake was obtained in ducks that received YFM-D (120 gm) than CON-D (112 gm). Subsequently, ducks fed YFM-D improved almost 5.24% and 4.26% higher egg mass and hen-day egg production than CON-D, respectively (p < 0.05). Though feed conversion ratio (egg basis) was unaffected (p > 0.05), ducks receiving YFM-D almost revealed 21%–22% higher body weight compared to CON-D (p < 0.05). Moreover, higher yolk weight, color, and Haugh unit were measured in ducks offered YFM-D than CON-D (p < 0.05), while YFM-D showed the propensity for better egg and albumen weight. However, shell weight and yield, albumen, and yolk yield were not varied between the two groups (p < 0.05). Conclusion: It may be concluded that a YFM-D enhanced hen day egg production at the early stage of laying while boosting live weight gain and enhancing egg quality. [Vet. Res. Notes 2024; 4(4.000): 43-47]

Abstract Background Broodiness significantly impacts poultry egg production, particularly notable in specific breeds such as the black-boned Silky, characterized by pronounced broodiness. An understanding of the alterations in ovarian signaling is essential for elucidating the mechanisms that influence broodiness. However, comparative research on the characteristics of long non-coding RNAs (lncRNAs) in the ovaries of broody chickens (BC) and high egg-laying chickens (GC) remains scant. In this investigation, we employed RNA sequencing to assess the ovarian transcriptomes, which include both lncRNAs and mRNAs, in eight Taihe Black-Bone Silky Fowls (TBsf), categorized into broody and high egg-laying groups. This study aims to provide a clearer understanding of the genetic underpinnings associated with broodiness and egg production. Results We have identified a total of 16,444 mRNAs and 18,756 lncRNAs, of which 349 mRNAs and 651 lncRNAs exhibited significantly different expression (DE) between the BC and GC groups. Furthermore, we have identified the cis-regulated and trans-regulated target genes of differentially abundant lncRNA transcripts and have constructed an lncRNA-mRNA trans-regulated interaction network linked to ovarian follicle development. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation analyses have revealed that DE mRNAs and the target genes of DE lncRNAs are associated with pathways including neuroactive ligand-receptor interaction, CCR6 chemokine receptor binding, G-protein coupled receptor binding, cytokine-cytokine receptor interaction, and ECM-receptor interaction. Conclusion Our research presents a comprehensive compilation of lncRNAs and mRNAs linked to ovarian development. Additionally, it establishes a predictive interaction network involving differentially abundant lncRNAs and differentially expressed genes (DEGs) within TBsf. This significantly contributes to our understanding of the intricate interactions between lncRNAs and genes governing brooding behavior.

Objective Our study examined the impact of propriety blends of Bacillus strain probiotics on the performance, egg quality, and faecal microflora of laying hens. Methods A total of 183 Institut de selection Animale (ISA) brown laying hens aged 23 weeks with an average body weight of 1,894±72 g were randomly allocated into 3 groups as control (corn-soybean meal based diet, CON), 0.5 g/kg Enterosure probiotics (ET1, 3×108 colony-forming unit [CFU]/kg feed), and 5 g/kg Enterosure probiotics (ET2, 3×109 CFU/kg feed) administered in mashed form. At the completion of each phase hen day egg production (HDEP), average egg weight (AEW), feed intake, and faecal microbiota were evaluated. Results HDEP and AEW were higher (p

Although crude protein (CP) is an important component of laying hen diets, there is still conflicting evidence on the impact of dietary CP on egg production, egg output and quality of stored eggs. This study aimed to evaluate the effects of various dietary CP levels, i.e. 172, 158, 148 and 113 g/kg, respectively, in isocaloric (11.50 MJ/kg metabolizable energy) diets on the internal egg quality of stored eggs obtained from the end of production laying hens. The impact of diets on egg production was also reported. Accordingly, one-hundred-sixty-eight Hy-line Brown laying hens were randomly allocated to four dietary treatments with seven replicates per treatment, from 75 to 83 w age. Three eggs per cage were collected during the last day of the experiment and stored for 8 weeks at 15 °C as egg quality measurements were taken every 4 weeks (0, 4 and 8 weeks after storage). Overall, feeding lower dietary CP reduced layers feed intake (P < 0.05), hen weight (P < 0.001) and egg production (P < 0.05). Lower dietary CP reduced egg weight (P < 0.001) but did not have an impact on the rest of the studied variables during storage. Birds fed diets containing field beans, sunflower or rapeseed meals produced eggs with denser coloured yolks (P < 0.001). Any observed changes in egg quality confirmed the expected effects of egg storage.

Abstract Background Schistosomiasis is a disease primarily caused by eggs laid by pathogens called schistosomes. Among the schistosome species infecting humans, Schistosoma japonicum possesses the largest fecundity; each adult female produces an average of 3500 eggs per day. The lack of proper culture conditions supporting continuous oviposition in vitro has precluded detailed investigation of mechanisms regulating sexual maturation and egg production in Schistosoma japonicum. Methods We optimized in vitro culture conditions by replacing reagents that are part of the classical ABC169 medium. Fast Blue BB staining and 4′,6-diamidino-2-phenylindole (DAPI) labeling were applied to observe the sexual development status of the females. In vitro RNA interference (RNAi) technology was used to validate the capability of the modified medium. The detection of male β-alanyl-tryptamine (BATT) was conducted using liquid chromatography–mass spectrometry (LC–MS). Results Both m-AB169 (1640) and AB169 (1640) media are capable of facilitating the sexual development of paired virgin female S. japonicum, as well as sustaining the mature reproductive organs and egg production of adult S. japonicum for at least 22 days in vitro. M-AB169 (1640) provided a more stable condition for supporting the sexual maturity of female S. japonicum, as evidenced by the consistent initiation of egg production compared with AB169 (1640). Through a comparative analysis of S. japonicum and S. mansoni in diverse media, we demonstrated that these closely related species display distinct demands for their sexual development and egg production, suggesting a potential influence of nutritional factors on the observed variations in host ranges among different schistosome species. Importantly, we successfully identified the presence of the pheromone β-alanyl-tryptamine (BATT) in S. japonicum, previously identified in S. mansoni, highlighting its conserved role in schistosome reproductive development. Through the employment of double-stranded RNA (dsRNA) treatment to silence two genes that are involved in either the male (gli1, glioma-associated oncogene homolog 1) or female (vf1, vitellogenic factor 1) side in male-induced female reproductive development of S. mansoni, we confirmed that the combination of m-AB169 (1640) and RNAi technology has the capacity to facilitate in vitro studies of S. japonicum’s reproductive and oviposition processes. Conclusions We developed a novel medium, m-AB169 (1640), that not only maintains the mature reproductive organs and continuous oviposition of adult female Schistosoma japonicum for up to 22 days but also supports the reproductive development and subsequent egg-laying of virgin females after pairing with male worms. This study provides a valuable in vitro platform for functional studies of the mechanisms underlying the fascinating biology of the female sexual development and egg production of S. japonicum, which may accelerate the development of new strategies targeting schistosome egg production. Graphical Abstract

This study aimed to gather published genetic parameter estimates for economically important traits in ducks through a meta-analysis utilizing the random-effects model. The present study used a dataset on genetic parameters of various duck traits, including 275 genetic correlation estimates and 233 heritability estimates from 31 studies published between 1988 and 2024. The heritability estimates for growth traits were generally low to high and varied from 0.154 (for body weight gain) to 0.405 (for body weight at first egg), respectively. Results showed that heritability estimates for egg production and quality traits were generally low to moderate, ranging from 0.119 (for egg shell strength) to 0.340 (for egg weight). The heritability estimates for feeding traits were generally moderate to high and varied from 0.266 (for residual feed intake) to 0.624 (for meal feed intake), respectively. The results indicate that there was a high genetic correlation (0.827, P < 0.05) between feed intake and residual feed intake, but low to moderate genetic correlations (P < 0.05) were found between feed intake and feed conversion ratio (0.318). The results of the current meta-analysis supported the hypothesis that these duck traits exhibit additive genetic variation. Genetic selection schemes for ducks may thus potentially take advantage of the available additive genetic variation in these traits. Furthermore, in cases where accurate estimates for economically significant traits in duck populations across the globe are unavailable, the average genetic parameter estimates presented in this meta-analysis can be used in breeding plans.

Blue-green eggs exhibit unique shell color; however, compared to commercial layers, blue-green eggshell chickens have lower egg production and lack uniform shell colors. Aiming to confirm the molecular mechanisms that affect shell color and egg production, this study collected the uteruses of 12 blue-green eggshell chickens (BG group) and six Hy-Line layers (Brown group), which had significantly different shell color indexes (SCI) and egg numbers at 300 days of age (EN300). Transcriptome sequencing and comparative analyses were subsequently performed. BG hens were divided into two groups for comparative analysis (BGblue vs. BGgreen and BGlow vs. BGhigh, respectively), based on the differences in SCI and EN300, respectively. The result of weighted gene co-expression network (WGCNA) analysis showed that the sequenced and mapped 16,785 genes were clustered into 18 modules, among which six modules with a total of 4270 genes were highly correlated with SCI and EN300 traits. Five hundred and eleven differentially expressed genes (DEGs) belonged to the six key modules. Through KEGG mapping, GO enrichment, and Cytoscape network analysis, nine Hub genes were tightly associated with SCI and EN300. The up-regulated genes were CCR2, CCR8, CD40LG, IL18RAP, INHBA, and P2RY13, while the down-regulated genes were ABCA13, ADCY2, and GRM1. Co-analyses with the results of comparisons between the BG subgroups revealed that the expression of solute carrier (SLC) proteins and ABC transporters were highly related to eggshell color, while cytokine-cytokine receptor interactions and neuroactive ligand-receptor interactions were key pathways affecting egg production. The expression of extracellular cytokines and membrane receptors were significantly up-regulated in low-yield chickens. The candidate DEGs and pathways found in the study will assist in clarifying the molecular mechanisms affecting shell color and egg production, and improve the breeding of blue-green eggshell chickens.

ABSTRACT: Egg production, regulated by multiple tissues, is among the most important economic traits in poultry. However, current research only focuses on the hypothalamic-pituitary-ovarian axis, ignoring the most important organ for substance metabolism in the body, the liver. Eggs are rich in lipids, proteins, and other nutrients, which are biosynthesized in the liver. Therefore, here the liver was included in the study of the hypothalamic-pituitary axis. This study used hypothalamus (HH_vs_LH), pituitary (HP_vs_LP), liver (HL_vs_LL), and ovary (HO_vs_LO) tissue samples from high- and low-laying Chengkou mountain chickens (CMC) for epihistological, transcriptome and metabolomic analyses aimed at improving the reproductive performance of CMC. The results showed that the liver of the high-laying group was yellowish, the cell boundary was clear, and the lipid droplets were evenly distributed. The ovaries of the high-laying group had a complete sequence of hierarchical follicles, which were rich in yolk. In contrast, the ovaries of the low-laying group were atrophic, except for a few small yellow follicles, and numerous primordial follicles that remained. The transcriptome sequences yielded 167.11 Gb of clean data, containing 28,715 genes. Furthermore, 285, 822, 787, and 1,183 differentially expressed genes (DEG) were identified in HH_vs_LH, HP_vs_LP, HL_vs_LL and HO_vs_LO and the DEGs significantly enriched 77, 163, 170, 171 pathways, respectively. Metabolome sequencing yielded 21,808 peaks containing 4,006 metabolites. The differential metabolite analysis yielded 343 and 682 significantly different metabolites (SDM) that significantly enriched 136 and 87 pathways in the liver and ovaries, respectively. A combined analysis of the transcriptome and metabolome of the liver and ovaries identified “CYP51A1-4α-carboxy-stigmasta7, 24(24(1))-dien-3β-ol” and “ACSS1B-estrone 3-sulfate” and other multiple gene-metabolite pairs. The DEGs in the hypothalamus and pituitary mainly enriched signaling transduction. In contrast, the DEGs and SDMs in the liver and ovaries mainly enriched the substance metabolism pathways: “gap junction”, “extracellular matrix (ECM)-receptor interaction”, “Steroid biosynthesis”, and “Steroid hormone biosynthesis”. These results suggest that the hypothalamic-pituitary axis may affect egg production mainly by regulating lipid metabolism in the liver and ovaries.

Management evaluation is essential to optimize finances by increasing egg production, survival rates, larval rearing, and nursery growth. Research aims to analyze production and financial performance in sustainable practices for producing hybrid grouper seeds such as cantang and cantik. The research was carried out for one year, from September 2022 to September 2023. Survey method with the selection of research locations carried out purposively utilized both primary and secondary data types. Primary data was collected through surveys using questionnaires, interviews, and direct observation of activities. Direct observations were conducted at egg production, hatchery, and nursery activities. Research shows that in one year the egg production is 97,300,000 cantang eggs and 17,800,000 cantik eggs. The final length mean harvested in the hatchery was 3.5 ± 0.28 cm with a survival rate of 8.3 ± 4.9 % for cantang hybrid grouper and 3.2 ± 0.25 cm with a survival rate of 10.1 ± 5.0 % for cantik hybrid grouper. The total length of the nursery harvested was 10.9 ± 0.30 cm with a survival rate of 71.5 ± 8.7 % for cantang hybrid grouper, and cantik hybrid grouper measured 10.1 ± 0.30 cm with a survival rate of 81.0±6.0 %. The highest net profit comes from nurseries, followed by larval rearing and egg production. Investments in egg production, larval rearing, and nursery businesses are financially advantageous, as both the R/C and B/C ratios > 1, demonstrating their economic viability. The increasing demand for hatcheries and seeds highlights the importance of successful egg production in fulfilling the growing needs of grouper hatcheries. Nursery operations play a crucial role in enhancing the success of grouper grow-out by improving survival rates and reducing rearing times. They focus on nurturing seeds from their early stages until they reach a suitable size and strength for the grow-out phase.