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28 results on '"Efstathiou, Antonia"'

1. Effect of isoquinoline alkaloids (IQs) on rainbow trout performance, serum parameters, antioxidant status, fillet fatty acid profile, intestinal and skin histomorphometry and expression of growth hormones genes

Author

Chantzi, Paraskevi, Vasilopoulou, Konstantina, Panitsidis, Ioannis, Bitchava, Konstantina, Efstathiou, Antonia, Dokou, Stella, Stylianaki, Ioanna, Tsoumani, Maria, Gouva, Evangelia, Michailidis, Georgios, Karagouni, Evdokia, Pastor, Anja, Wall, Sophie-Charlotte, and Giannenas, Ilias

Published
2024
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2. Seroprevalence of Toxoplasma gondii among HIV Positive Patients under Surveillance in Greek Infectious Disease Units: A Screening Study with Comparative Evaluation of Serological Methods

Author

Voyiatzaki, Chrysa, primary, Zare Chormizi, Apollon Dareios, additional, Tsoumani, Maria E., additional, Efstathiou, Antonia, additional, Konstantinidis, Konstantinos, additional, Chaniotis, Dimitrios, additional, Chrysos, Georgios, additional, Argyraki, Aikaterini, additional, Papastamopoulos, Vasilios, additional, and Kotsianopoulou, Marika, additional

Published
2024
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3. Serological Screening and Risk Factors Associated with Leishmania infantum Positivity in Newly Diagnosed HIV Patients in Greece.

Author

Voyiatzaki, Chrysa, Zare Chormizi, Apollon Dareios, Tsoumani, Maria E., Efstathiou, Antonia, Konstantinidis, Konstantinos, Chrysos, Georgios, Argyraki, Aikaterini, Papastamopoulos, Vasileios, Papageorgiou, Effie G., and Kotsianopoulou, Marika

Subjects
NEGLECTED diseases, ENZYME-linked immunosorbent assay, MEDICAL screening, LEISHMANIASIS, ANTIBODY titer, LEISHMANIA infantum, HIV
Abstract

A serological screening was conducted to detect IgG antibodies against Leishmania infantum (L. infantum) in newly diagnosed human immunodeficiency virus (HIV) patients in Greece. The study also examined potential risk factors and the agreement of commercially available serological methods. IgG antibodies against L. infantum were detected using enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescence antibody test (IFAT), and Western blot (WB). Out of 155 samples, 14 (9.0%) tested positive for IgG antibodies against L. infantum using at least two methods. Statistical analysis showed substantial agreement between WB and IFAT methods (Cohen's kappa = 0.75) but moderate overall agreement among the three methods (Fleiss' kappa = 0.42). Additionally, HIV+ intravenous drug users faced 3.55 times (p = 0.025) higher risk of testing positive for L. infantum IgG, positing that anthroponotic transmission between these patients is a plausible hypothesis based on existing literature. Non-invasive and cost-effective techniques are preferred to detect asymptomatic infections, and leishmaniasis screening should be conducted immediately after HIV diagnosis in endemic regions to enable prophylactic treatment for leishmaniasis in addition to antiretroviral therapy. To maximize sensitivity, performing at least two different serological methods for each patient is recommended. [ABSTRACT FROM AUTHOR]

Published
2024
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5. Nano-Oregano Essential Oil Improves Rainbow Trout’s (Oncorhynchus mykiss) Growth Performance, Oxidative Status, Fatty Acid Profile of Fillet, Affects Gene Expression and Supports Skin and Intestinal Histomorphometry

Author

Dokou, Stella, primary, Bitchava, Konstantina, additional, Stylianaki, Ioanna, additional, Chantzi, Paraskevi, additional, Efstathiou, Antonia, additional, Vasilopoulou, Konstantina, additional, Tsoumani, Maria, additional, Gouva, Evangelia, additional, Michailidis, Georgios, additional, Prafulla, Kumar, additional, Karagouni, Evdokia, additional, and Giannenas, Ilias, additional

Published
2023
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14. Indirubin Analogues Inhibit Trypanosoma brucei Glycogen Synthase Kinase 3 Short and T. brucei Growth

Author

Efstathiou, Antonia, primary, Gaboriaud-Kolar, Nicolas, additional, Myrianthopoulos, Vassilios, additional, Vougogiannopoulou, Konstantina, additional, Subota, Ines, additional, Aicher, Stephanie, additional, Mikros, Emmanuel, additional, Bastin, Philippe, additional, Skaltsounis, Alexios-Leandros, additional, Soteriadou, Ketty, additional, and Smirlis, Despina, additional

Published
2019
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15. Indirubin derivatives are potent and selective anti-Trypanosoma cruzi agents

Author

Efstathiou, Antonia, primary, Meira, Cássio Santana, additional, Gaboriaud-Kolar, Nicolas, additional, Bastos, Tanira Matutino, additional, Rocha, Vinícius Pinto Costa, additional, Vougogiannopoulou, Konstantina, additional, Skaltsounis, Alexios-Leandros, additional, Smirlis, Despina, additional, and Soares, Milena Botelho Pereira, additional

Published
2018
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18. Three new trixane glycosides obtained from the leaves of Jungia sellowii Less. using centrifugal partition chromatography

Author

Azevedo, Luíse, primary, Faqueti, Larissa, additional, Kritsanida, Marina, additional, Efstathiou, Antonia, additional, Smirlis, Despina, additional, Franchi, Gilberto C, additional, Genta-Jouve, Grégory, additional, Michel, Sylvie, additional, Sandjo, Louis P, additional, Grougnet, Raphaël, additional, and Biavatti, Maique Weber, additional

Published
2016
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21. An inhibitor-driven study for enhancing the selectivity of indirubin derivatives towards leishmanial Glycogen Synthase Kinase-3 over leishmanial cdc2-related protein kinase 3

Author

Efstathiou, Antonia, primary, Gaboriaud-Kolar, Nicolas, additional, Smirlis, Despina, additional, Myrianthopoulos, Vassilios, additional, Vougogiannopoulou, Konstantina, additional, Alexandratos, Alexandros, additional, Kritsanida, Marina, additional, Mikros, Emmanuel, additional, Soteriadou, Ketty, additional, and Skaltsounis, Alexios-Leandros, additional

Published
2014
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25. The loss of virulence of histone H1 overexpressing L eishmania donovani parasites is directly associated with a reduction of HSP83 rate of translation.

Author

Alexandratos, Alexandros, Clos, Joachim, Samiotaki, Martina, Efstathiou, Antonia, Panayotou, George, Soteriadou, Ketty, and Smirlis, Despina

Subjects
LEISHMANIA, INTRACELLULAR pathogens, SAND flies, MAMMALS, CANCER chemotherapy, DRUG resistance
Abstract

Overexpression of L eishmania histone H1 ( LeishH1) was previously found to cause a promastigote-to-amastigote differentiation handicap, deregulation of cell-cycle progression, and loss of parasite infectivity. The aim of this study was to identify changes in the proteome of LeishH1 overexpressing parasites associated with the avirulent phenotype observed. 2 D-gel electrophoresis analysis revealed only a small protein subset of differentially expressed proteins in the LeishH1 overexpressing promastigotes. Among these was the chaperone HSP83, known for its protective role in L eishmania drug-induced apoptosis, which displayed lower translational rates. To investigate if the lower expression levels of HSP83 are associated with the differentiation handicap, we assayed the thermostability of parasites by subjecting them to heat-shock (25°C→37°C), a natural stress-factor occurring during stage differentiation. Heat-shock promoted apoptosis to a greater extent in the LeishH1 overexpressing parasites. Interestingly, these parasites were not only more sensitive to heat-shock but also to drug-induced [ Sb( III)] cell-death. In addition, the restoration of HSP83 levels re-established drug resistance, and restored infectivity to LeishH1 overexpressing parasites in the murine J774 macrophage model. Overall, this study suggests that LeishH1 levels are critical for the parasite's stress-induced adaptation within the mammalian host, and highlights the cross-talk between pathways involved in drug resistance, apoptosis and virulence. [ABSTRACT FROM AUTHOR]

Published
2013
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26. Biochemical and Genetic Evidence for the Presence of Multiple Phosphatidylinositol- and Phosphatidylinositol 4,5-Bisphosphate-Specific Phospholipases C in Tetrahymena

Author

Leondaritis, George, Sarri, Theoni, Dafnis, Ioannis, Efstathiou, Antonia, and Galanopoulou, Dia

Abstract

Eukaryotic phosphoinositide-specific phospholipases C (PI-PLC) specifically hydrolyze phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2], produce the Ca2+-mobilizing agent inositol 1,4,5-trisphosphate, and regulate signaling in multicellular organisms. Bacterial PtdIns-specific PLCs, also present in trypanosomes, hydrolyze PtdIns and glycosyl-PtdIns, and they are considered important virulence factors. All unicellular eukaryotes studied so far contain a single PI-PLC-like gene. In this report, we show that ciliates are an exception, since we provide evidence that Tetrahymena species contain two sets of functional genes coding for both bacterial and eukaryotic PLCs. Biochemical characterization revealed two PLC activities that differ in their phosphoinositide substrate utilization, subcellular localization, secretion to extracellular space, and sensitivity to Ca2+. One of these activities was identified as a typical membrane-associated PI-PLC activated by low-micromolar Ca2+, modestly activated by GTPγS in vitro, and inhibited by the compound U73122 [1-(6-{[17β-3-methoxyestra-1,3,5(10)-trien-17-yl]amino}hexyl)-1H-pyrrole-2,5-dione]. Importantly, inhibition of PI-PLC in vivo resulted in rapid upregulation of PtdIns(4,5)P2levels, suggesting its functional importance in regulating phosphoinositide turnover in TETRAHYMENA: By in silico and molecular analysis, we identified two PLC genes that exhibit significant similarity to bacterial but not trypanosomal PLC genes and three eukaryotic PI-PLC genes, one of which is a novel inactive PLC similar to proteins identified only in metazoa. Comparative studies of expression patterns and PI-PLC activities in three T. thermophila strains showed a correlation between expression levels and activity, suggesting that the three eukaryotic PI-PLC genes are functionally nonredundant. Our findings imply the presence of a conserved and elaborate PI-PLC-Ins(1,4,5)P3-Ca2+regulatory axis in ciliates.

Published
2010

27. Indirubin Analogues Inhibit Trypanosoma bruceiGlycogen Synthase Kinase 3 Short and T. bruceiGrowth

Author

Efstathiou, Antonia, Gaboriaud-Kolar, Nicolas, Myrianthopoulos, Vassilios, Vougogiannopoulou, Konstantina, Subota, Ines, Aicher, Stephanie, Mikros, Emmanuel, Bastin, Philippe, Skaltsounis, Alexios-Leandros, Soteriadou, Ketty, and Smirlis, Despina

Abstract

The protozoan parasite Trypanosoma bruceiis the causative agent of human African trypanosomiasis (HAT). The disease is fatal if it remains untreated, whereas most drug treatments are inadequate due to high toxicity, difficulties in administration, and low central nervous system penetration.

Published
2019
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28. A Radioactive-free Kinase Inhibitor Discovery Assay Against the Trypanosoma brucei Glycogen Synthase Kinase-3 short ( Tb GSK-3s).

Author

Efstathiou A and Smirlis D

Abstract

The identification of small molecules possessing inhibitory activity in vitro , against a given target kinase, is the first step in the drug discovery process. Herein, we describe a non radioactive protocol using luciferase-based ATP assay for the identification of inhibitors for the short isoform of the Trypanosoma brucei 's Glycogen Synthase Kinase-3 ( Tb GSK-3s). Tb GSK-3s represents a potential drug target as it is essential for parasite survival. Small molecules used in our study are indirubin analogues possessing substitutions in different positions in the bis-indole backbone. Presently, the standard laboratory practice for the kinase assays is the incorporation of radiolabeled phosphate from [gamma- 32 P]ATP as the efforts for developing non-radioactive assays (ELISA-based assays, fluorescence quenching assays, etc. ) exhibit limitations such as lack in sensitivity or limitations for broad applications. This protocol can be a useful starting point for lead discovery, as it surpasses the drawbacks of radioactive kinase assays and it allows for relatively sensitive measurements of kinase inhibition for Tb GSK-3s., Competing Interests: Competing interestsBoth authors declare that they have no conflicts of interest., (Copyright © 2020 The Authors; exclusive licensee Bio-protocol LLC.)

Published
2020
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