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Post-fire rangeland management recommends rest from grazing to allow forage regrowth and litter accumulation. In the first year after wildfire in the mixed-grass Prairie, we examined forage and litter mass responses to variable timing of defoliation in burned and non-burned areas. Total forage biomass did not differ between burned and non-burned areas by the second growing season. The July defoliation in both burned and non-burned areas reduced total forage biomass. Litter mass was decreased by wildfire and was further reduced by all defoliation treatments.

Agroforestry systems (AFS) contribute to carbon (C) sequestration and reduction in greenhouse gas emissions from agricultural lands. However, previously understudied differences among AFS may underestimate their climate change mitigation potential. In this 3-year field study, we assessed various C stocks and greenhouse gas emissions across two common AFS (hedgerows and shelterbelts) and their component land uses: perennial vegetated areas with and without trees (woodland and grassland, respectively), newly planted saplings in grassland, and adjacent annual cropland in central Alberta, Canada. Between 2018 and 2020 (~April-October), nitrous oxide emissions were 89% lower under perennial vegetation relative to the cropland (0.02 and 0.18 g N m

This is the replication data associated with our article in "Journal of Ecology" titled "Multi-year drought alters plant species composition more than productivity across northern temperate grasslands". Please see the ReadMe file for details on dataset contents.

Significant interest exists in the potential for specialized grazing systems, including adaptive multipaddock (AMP) grazing, to enhance grassland health and function. However, specific pasture management practices associated with AMP grazing at the ranch level remain poorly understood in comparison with more regionally representative management systems. As part of a larger study examining grazing effects on soil carbon, greenhouse gases, and other ecosystem attributes, here we report on differences in disturbance history and grazing management practices on a sample of AMP operators and their neighboring (n-AMP) ranches at 32 paired sites across the prairie provinces of western Canada. Most ranches studied (77.5%) relied on pastures composed of introduced (seeded) forage. On average, the AMP ranches surveyed were larger in size, supported greater animal numbers, and were more likely to use seeded forages comprising diverse mixes. Relative to n-AMP ranches, AMP ranches used 18.6-fold higher average stock densities in smaller paddocks (22.3 vs. 120.7 ha) while grazing over a grazing season that was 76 d longer, although computed stocking rates remained similar (P ≥ 0.10). AMP operators specifically used much shorter grazing periods (2.8 d) during the early growing season (i.e., before August 1) that were followed by a prolonged rest period (69 d) and could be used to compute a rest-to-grazing ratio for the first half of the grazing season for all ranches. This ratio, along with cattle stock density computed at the pasture scale, exhibited the greatest potential to differentiate the two groups of ranchers. Finally, both groups, and in particular ranchers within the AMP group, demonstrated high variability in management practices among individual operators, highlighting the importance of using specific management metrics rather than generalized descriptors of “grazing system type” to interpret their influence.

Wetland decline under post-European settlement and land use change across western Canada has led to mitigation strategies, including wetland creation. Created wetlands can trigger environmental change, including woody species encroachment, in turn altering vegetation and soil. We quantify changes in shrub abundance from prior to wetland creation (1949) until 60 years later (2012) within a Mixedgrass ecosystem of the Verger watershed in Alberta, Canada. In addition, we compare remaining grassland with areas colonized by shrubland on similar ecosites for differences in (1) plant composition, including native and introduced flora, (2) herbage yield and forage accessibility for livestock, and (3) soil properties (surface organic depth, bulk density, mineral nitrogen (N), and carbon (C) concentration). Repeat photos show Shepherdia argentea shrublands increased from 0 to 88 ha (to 1.15% of study area) following wetland creation, with the greatest increase in the last 20 years. Relative to grasslands, shrublands had lower total plant diversity but greater presence of introduced plant species. Shrub patches were 94% lower in herbaceous production, with 77% of shrublands non-utilized by cattle, collectively leading to reduced grazing capacity. Relative to grasslands, shrublands had a thicker soil surface mulch layer, and where cattle were present, had increased mineral soil N and C. Overall, shrub encroachment following wetland creation has markedly altered vegetation and soils in this once grassland landscape, with negative impacts on native plant diversity, herbage production and forage accessibility, and has implications for the management of shrub encroachment.

Grassland management aimed at enhancing carbon (C) in soil is an important tool in mitigation of rising atmospheric CO₂, yet little is known of how grassland soil C changes with livestock stocking rate (SR). We relate soil organic and inorganic C mass (t ha⁻¹ to 60 cm depth) with cattle stocking over periods of 7–27 year for 32 paddocks distributed across nine community pastures in the mixed‐grass prairie of Saskatchewan, Canada. Initial analysis comparing Akaike information criterion models showed that cattle SR explained a greater proportion of variance in soil C, particularly soil organic C, than rainfall. Soil organic C mass increased with cattle SR (R² = .293; p = .001), even when the latter was normalized to account for differences in vegetation composition and growing conditions among pastures. Normalized SR varied from 0.49 to 2.30 times recommended levels, over which SOC increased from 24.7 to 57.4 t ha⁻¹. Increases in soil organic C under greater stocking coincided with increased abundance of introduced vegetation, particularly the rhizomatous grass Poa pratensis. Inorganic soil C accounted for 34.6% of total soil C, being particularly large below 30 cm soil depth, but did not vary with stocking rate. These findings indicate that both organic and inorganic C are important pools of C in northern temperate grassland soils, with soil organic C positively associated with long‐term cattle SR. Further studies are recommended to understand more fully the mechanisms regulating grazing impacts on soil C mass in northern temperate grasslands.

While northern temperate grasslands are important for supporting beef production, it remains unclear how grassland above- and belowground biomass responds to long-term cattle grazing. Here, we use a comprehensive dataset from 73 grasslands distributed across a broad agro-climatic gradient to quantify grassland shoot, litter, and shallow (top 30 cm) root biomass in areas with and without grazing. Additionally, we relate biomass to soil carbon (C) concentrations. Forb biomass was greater (p −1 for grazed vs. 2210 kg ha−1 for non-grazed; p > 0.05). Forb crude protein concentrations were lower (p −1) and without (7130 kg ha−1) grazing (p > 0.05). Surface mineral soil C concentrations were positively related to peak grassland biomass, particularly total (above + belowground) biomass, and with increasing forb biomass in grazed areas. Finally, total aboveground shoot biomass and soil C concentrations in the top 15 cm of soil were both positively related to the proportion of introduced plant diversity in grazed and non-grazed grasslands. Overall, cattle grazing at moderate stocking rates had minimal impact on peak grassland biomass, including above- and belowground, and a positive contribution exists from introduced plant species to maintaining herbage productivity and soil C.

Studies examining the influence of disturbance and management history on pasture soils across a large sampling area are uncommon. We report on the soil properties found in 102 northern temperate pastures sampled in central Alberta, Canada, and relate these attributes to ongoing pasture management practices compiled from producer surveys and aboveground measures of rangeland health (RH). Tame pastures, typically seeded to introduced forages, were associated with higher soil fertility (total carbon, nitrogen, and organic matter) than semi-native grasslands, which were associated with coarse-textured soils. Soil properties remained independent of most grazing and pasture management practices, including the grazing systems, class of livestock, fertilization, and stocking rate. However, manure application, often combined with harrowing, was associated with improved soil fertility and increased electrical conductivity (salinity). Soils with a fire history reported by land managers, largely in the Boreal natural region, were characterized by a greater soil C:N ratio. Soil surface properties (litter cover, litter depth, and bare soil) were responsive to grazing management, with growing season and year-round grazing associated with a thinner litter layer having less cover, and bare ground twice as high under continuous grazing compared with pastures rotationally grazed. Further, variation in soil surface cover was associated with contrasting RH classes (healthy, healthy with problems, and unhealthy), whereas soil attributes remained unrelated to RH. This study demonstrates that soils within these northern temperate grasslands are relatively insensitive to many pasture management practices, and highlights that existing RH assessments may provide limited insight into differences in mineral soil properties.

A survey of Alberta beef producers was conducted at sites overlapping with a province-wide network of permanent biodiversity monitoring plots to characterize focal pastures and their management, including estimates of stocking rates. Overall, greater stocking rates were reported in the boreal compared with the parkland and grassland natural regions, coinciding with an increased reliance on tame forage on relatively small land areas of largely deeded land. Higher stocking rates were also associated with earlier starting dates of grazing in the season, higher mean annual precipitation, and lower mean annual temperature.

The spatial effects of vegetation control on early tree growth were investigated in central Alberta, Canada, for four years after the establishment of hybrid poplar plantations including the two cl...

Management intensive grazing (MIG) may not maximize plant productivity on rangelands because of morphophysiological traits of grassland vegetation. We examined defoliation and moisture effects on the biomass yield of rhizomatous and caespitose grass pairs that were either phylogenetically similar or of similar agroclimatic adaptation, including two agronomic grasses. From relatively low to high moisture regime adaptation, species pairs included western wheatgrass (Pascopyrum smithii [Rydb.] A. Love) and needle-and-thread (Hesperostipa comata [Trin. & Rupr.] Barkw.), northern wheatgrass (Elymus lanceolatus [Scribn. & J.G. Sm.]) and western porcupine grass (H. curtiseta [Hitchc.] Barkw.), plains and foothills rough fescue (Festuca hallii [Vasey] and F. campestris Rydb.), and smooth and meadow brome (Bromus inermis Leyss. and B. riparius Rehm). Response variables were shoot yield, root-shoot ratio, and water-use efficiency. We hypothesized that caespitose grasses, regardless of their origin or adaptation to agroclimate regime, would respond more determinately in biomass accumulation. Defoliation effects on shoot biomass were more pronounced under high moisture. Low intensity − high frequency defoliation yielded similarly to deferred controls in all grasses, and the same was true for high-intensity − low-frequency (HILF) defoliation in 1 rhizomatous grass. Three of the 4 rhizomatous grasses and 1 caespitose grass yielded greater under HILF defoliation compared with high-intensity − high-frequency defoliation. Caespitose grasses allocated more biomass to roots under low moisture conditions. Water-use efficiency decreased under high moisture conditions and more intense and/or frequent defoliation and peaked in agronomic grasses. Overall, our results suggested that growth patterns corresponded more with phylogenetic similarity as opposed to growth form. A conceptual model from these results showed that across all species, only the introduced bromes generated greater biomass under HILF defoliation, and this may explain why past research consistently concludes that MIG does not enhance plant productivity on rangelands.

Adaptive multi-paddock (AMP) grazing is a form of rotational grazing in which small paddocks are grazed with high densities of livestock for short periods, with long recovery periods prior to regrazing. We compared the fluxes of greenhouse gases (GHGs), including carbon dioxide (CO2), methane (CH4), and nitrous oxide (N2O), from soils of AMP-grazed grasslands to paired neighboring non-AMP-grazed grasslands across a climatic gradient in Alberta, Canada. We further tested GHG responses to changes in temperature (5 &deg, C vs. 25 &deg, C) and moisture levels (permanent wilting point (PWP), 40% of field capacity (0.4FC), or field capacity (FC)) in a 102-day laboratory incubation experiment. Extracellular enzyme activities (EEA), microbial biomass C (MBC) and N (MBN), and available-N were also measured on days 1, 13, and 102 of the incubation to evaluate biological associations with GHGs. The 102-day cumulative fluxes of CO2, N2O, and CH4 were affected by both temperature and moisture content (p &lt, 0.001). While cumulative fluxes of N2O were independent of the grazing system, CH4 uptake was 1.5 times greater in soils from AMP-grazed than non-AMP-grazed grasslands (p &lt, 0.001). There was an interaction of the grazing system by temperature (p &lt, 0.05) on CO2 flux, with AMP soils emitting 17% more CO2 than non-AMP soils at 5 &deg, C, but 18% less at 25 &deg, C. The temperature sensitivity (Q10) of CO2 fluxes increased with soil moisture level (i.e., PWP &lt, 0.4FC &le, FC). Structural equation modelling indicated that the grazing system had no direct effect on CO2 or N2O fluxes, but had an effect on CH4 fluxes on days 1 and 13, indicating that CH4 uptake increased in association with AMP grazing. Increasing soil moisture level increased fluxes of GHGs&mdash, directly and indirectly&mdash, by influencing EEAs. Irrespective of the grazing system, the MBC was an indirect driver of CO2 emissions and CH4 uptake through its effects on soil EEAs. The relationships of N-acetyl-&beta, glucosaminidase and &beta, glucosidase to N2O fluxes were subtle on day 1, and independent thereafter. AMP grazing indirectly affected N2O fluxes by influencing N-acetyl-&beta, glucosaminidase on day 13. We conclude that AMP grazing has the potential to mitigate the impact of a warmer soil on GHG emissions by consuming more CH4 compared to non-AMP grazing in northern temperate grasslands, presumably by altering biogeochemical properties and processes.

Creation and use of multi-use trails are increasing throughout grasslands of North America. While the direct and indirect ecological impacts of multi-use trails are generally understood, their specific impacts on adjacent grassland conservation require further assessment. Traditional scientific methods of quantifying trail impacts are often prohibitively costly in terms of required time, expertise, and equipment. Here, we evaluate the utility of a rapid assessment methodology—combining rangeland health protocols for grasslands with publicly available Google Earth mapping technologies—for capturing trail impacts as a function of distance from trail in a multi-use natural area in southwestern Alberta, Canada. Our methodology successfully detected a positive relationship between rangeland health scores and increasing distance from trail, indicating its viability as a rapid assessment tool. Second, this methodology was sensitive enough to allow the development of a more generalized statistical model demonstrating that rangeland health was best explained by a combination of slope, aspect, plant community type, and distance from trail. Combined, we suggest the limited costs of this method, combined with its ability to detect indirect impacts of trails on the health of adjacent grasslands, indicate this tool has potential utility for land managers where resources are limited. More specifically, we suggest this grassland health protocol can be highly effective as a first “rapid assessment,” prior to investing in more traditional ecological methodologies.Jessica Grenke, James F. Cahill, and Edward W. Bork. 2018. “Quantification of Multi-Use Trail Effects Using a Rangeland Health Monitoring Approach and Google Earth.” Natural Areas Journal 38(5):370-379.

Grasslands are declining worldwide and are often impacted by industrial activities, including infrastructure development. Current best management practices for low-disturbance development on grasslands include the use of wooden access mats as temporary work platforms and roadways to mitigate soil compaction and rutting due to heavy traffic. We assessed the impacts of heavy traffic (TON), and the impacts of the same heavy equipment driven over top of access mats (AM), on soil physical, hydrological, and nutrient responses in sandy and loamy soils in the Dry Mixedgrass prairies over a 2-year period. We also assessed how the timing (early vs. late in the growing season) and duration (6 vs. 12 vs. 24 weeks) of AM and TON affected the same metrics. Compared to undisturbed soils, TON increased soil penetration resistance (15 cm depth) up to 93% in loamy and up to 101% in sandy soils, and decreased water infiltration rates from 53 to 71%, respectively. Notably, the negative impacts of TON on soil physical characteristics and hydrology were larger in sandy vs. loamy soils, and when moist soils were exposed to traffic early in the growing season. AMs were effective at mitigating soil compaction from industrial traffic when used on sandy soils. However, AM use increased the supply of total nitrogen and other plant macro- and micro-nutrients, particularly in soils subject to longer (12-24 wk) mat placement. Results indicate TON may have long-lasting effects on grassland (particularly sandy) soils, and that AM use represents an effective tool to mitigate traffic impacts. Further, early-season traffic should be avoided when soils are moist (whether with AM or not), and AMs should be placed on soils for limited durations (≤6 wk) to minimize potential nutrient losses.

Applying organic amendments to soil can increase soil organic carbon (SOC) storage and reduce greenhouse gas (GHG) emissions generated by agriculture, helping to mitigate climate change. However, it is necessary to determine which type of amendment produces the most desirable results. We conducted a 3-y field study comparing one-time addition of manure compost and its biochar derivative to a control to assess their effects on SOC and GHG emissions at ten annually cropped sites in central Alberta, Canada. Manure compost and biochar were applied at equivalent carbon rates (7 Mg ha−1) and tilled into the surface 10 cm of soil. Two years post-treatment, biochar addition increased surface (0–10 cm) SOC by 12 and 10 Mg ha−1 relative to the control and manure addition, respectively. Therefore, biochar addition led to the sequestration of SOC at a rate of 2.5 Mg ha−1 y−1 relative to the control. No treatment effect on deeper (10–100 cm) or cumulative SOC was found. In 2018 and 2019, manure addition increased cumulative GHG (sum of CO2, CH4, and N2O) emissions by 33%, on average, due to greater CO2 emissions relative to both the control and biochar addition. In contrast, in 2020, biochar addition reduced cumulative GHG emissions by an average of 21% due to lower CO2 emissions relative to both the control and manure addition. Our study shows that the application of biochar, rather than its manure compost feedstock, increased surface SOC sequestration and had either no effect on (first two years) or reduced GHG emissions (year three) relative to the control. We recommend that policy and carbon sequestration initiatives focus on optimizing biochar production-application systems to fully realize the potential of biochar application as a viable climate change mitigation practice in agriculture.

Soil microorganisms play critical roles in maintaining ecological functions of the northern mixed-grass prairie. While defoliation regimes and elevated precipitation affect plant community composition and primary productivity in these grasslands, their effects on soil microbiome and interactome have not been studied. We examined the response of soil microbiome and interactome to long-term (2010–2015) treatments of varying defoliation regimes (low vs. high intensity and low vs. high frequency) and two precipitation conditions (ambient vs. elevated) on two contrasting sites (a dry vs. a mesic site) in a northern mixed-grass prairie. High intensity defoliation reduced alpha-diversity and altered the beta-diversity of soil microbial communities in the mesic site but not in the dry site, while elevated precipitation reduced alpha-diversity and altered the beta-diversity of soil microbial communities in both sites. Defoliation and precipitation did not show interaction effects on overall alpha- and beta-diversity, but did combine to influence the interactome network. High intensity defoliation promoted the oligotrophic genera Spartobacteria (Verrucomicrobia), Pseudonocardia (Actinobacteria), and Conexibacter (Actinobacteria), while elevated precipitation promoted the copiotrophic genera Nitrososphaera (Thaumarchaeota), Anderseniella (Proteobacteria), Sphingomonas (Proteobacteria), and Acidobacteria Gp16 (Acidobacteria). High intensity defoliation increased the number of linkages for Alphaproteobacteria and Acidobacteria, and decreased the number of linkages for Gemmatimonadetes and Spartobacteria in the interactome networks. However, elevated precipitation increased the number of linkages for Actinobacteria and Gemmatimonadetes and decreased the number of linkages for Spartobacteria and Acidobacteria. Our results show that defoliation intensity and elevated precipitation affected the microbiome and associated interactome network within soils of the northern mixed-grass prairie by affecting different functional taxonomic groups, indicating distinct scenarios for defoliation and precipitation in affecting soil microbial communities.

Little information exists on the management and range health (RH) of northern temperate pastures, where health is defined as the ability to sustain ecosystem function. We surveyed 102 pastures for RH and their associated managers during 2012–2013 across central Alberta, Canada. Pastures were generally diverse mixes of introduced grasses and contained few legumes, despite most (55%) being seeded to legumes. The majority of pastures were healthy (mean RH score = 78.8% ± 1.3%), with 2.9% having scores −1] compared with those lacking cultivation (2.14 ± 2.91 AUM ha−1). Farms with horses or mixed livestock tended to practice year-round continuous grazing and supplemental hay feeding. Stocking rates on continuous and rotationally grazed pastures were similar regardless of grazing season, with the highest stocking on pastures used year-round (19.54 ± 2.03 AUM ha−1) or throughout the dormant season (20.29 ± 3.10 AUM ha−1). Use of management inputs was variable, with manuring and harrowing common and fertilizing, over-seeding, and aerating infrequent. Herbicide use was reported on 15.7% of pastures, despite 83.3% containing noxious weeds. Use of prescribed fire was rare, although 36.3% of pastures had evidence of fire. Industrial disturbances were reported on 48.3% of pastures. Overall, these results indicate that these pastures experience complex management and the limited decline in RH is due to high stocking under year-long grazing.

Our understanding of the effect of agroforestry systems on soil organic carbon (SOC) is largely limited to the upper layer of the mineral soil, while LFH (litter, partially decomposed litter and humus) and deeper soil layers are poorly studied. In this study, the effects of three different agroforestry systems (hedgerow, shelterbelt, and silvopasture) and their component land-cover types (treed area and adjacent herbland) on SOC stock in LFH and mineral soil layers (0–75 cm) were investigated across 36 sites in central Alberta, Canada. The SOC stock of mineral soil (0–75 cm) was not affected by agroforestry systems but by land-cover type. The treed area had greater (p

Little is known about the specific role of exotic species on measures of grassland plant diversity, including how this may vary with climatic conditions or large mammal herbivory. This study examined vegetation responses to long-term livestock grazing, including plant richness and diversity, as well as the contribution of exotic species to these metrics, across a network of 107 northern temperate grasslands in Alberta, Canada, spanning a broad aridity gradient. Exposure to grazing modestly increased plant richness, but did not alter Shannon’s diversity, Simpson’s diversity, or evenness, suggesting stability in floral diversity relative to grazing. However, grazing did increase grass cover while reducing shrub cover, the latter of which was only apparent in mesic grasslands. Unlike total plant diversity, exotic species richness and cover, together with exotic plant contributions to diversity, varied jointly with grazing and aridity. While long-term grazing increased exotic species, this response was most apparent in wetter areas, and non-grazed grasslands remained more resistant to the presence of exotics. Several exotic species were positive indicators of grazing in wetter grasslands, and coincided with lower native species cover, indicating grazing may be facilitating a shift from native to exotic vegetation under these conditions. Overall, our results indicate that while long-term grazing has altered the composition and cover of certain functional groups, including favoring exotics and minimizing woody vegetation in mesic areas, overall changes to plant diversity were limited. Additionally, these findings suggest that semi-arid northern temperate grasslands remain relatively resistant to grazing effects, including their susceptibility to exotic plant encroachment. These results improve our understanding of how ongoing grazing exposure may impact grassland diversity, including efforts to conserve native vegetation, as well as the important role of climate in altering fundamental grassland responses to grazing.

Adaptive multi-paddock (AMP) grazing, a grazing system in which individual paddocks are grazed for a short duration at a high stock density and followed by a long rest period, is claimed to be an effective tool to sustainably manage and improve grasslands and enhance their ecosystem services. However, whether AMP grazing is superior to conventional grazing (n-AMP) in reducing soil greenhouse gas (GHG) emissions is unclear. Here, we measured CO2, CH4, and N2O fluxes between August 2017 and August 2019 in 12 pairs of AMP vs. n-AMP ranches distributed across an agro-climatic gradient in Alberta, Canada. We found that field GHG fluxes did not differ between AMP and n-AMP grazing systems, but instead were regulated by specific management attributes, environmental conditions, and soil properties, including cattle stocking rate, cultivation history, soil moisture content, and soil bulk density. Specifically, we found that seasonal mean CO2 emissions increased with increasing cattle stocking rates, while CH4 uptake was lower in grasslands with a history of cultivation. Seasonal mean CO2 emissions increased while CH4 uptake decreased with increasing soil moisture content. In addition, CH4 uptake decreased with increasing soil bulk density. Observed N2O emissions were poorly predicted by the management, environmental conditions, and soil properties investigated in our study. We conclude that AMP grazing does not have an advantage over n-AMP grazing in reducing GHG fluxes from grasslands. Future efforts to develop optimal management strategies (e.g., the use of sustainable stocking rates and avoided cultivation) that reduce GHG emissions should also consider the environmental conditions and soil properties unique to every grassland ecosystem.

The maintenance of hydrologic function on grazing lands is an important management objective to sustain forage production during low moisture supply, safeguard other ecosystem goods and services and build resilience to a warming climate. Hydrologic function can be influenced by grazing patterns, as represented by variation in the timing, intensity and frequency of livestock use. While rotational, adaptive grazing (a short-duration, multi-paddock grazing system that emphasises plant recovery between grazing events) is growing in popularity and has the potential to influence grassland hydrological processes such as water infiltration, few studies have comprehensively examined infiltration in relation to on-ranch grazing practices. We examined water infiltration in grasslands on 52 ranches (set up as matched pairs) to examine whether adaptive grazing alters water infiltration in the Great Plains of western Canada, as compared to conventional grazing management employed on neighbouring ranches. We also used producer survey information to test for the influence of ongoing nuanced grazing practices on water infiltration rates, over and above the biophysical effects of soil texture, soil bulk density and plant litter, as well as cultivation history and climate. Overall, adaptive grazing, and specifically the use of higher rest-to-grazing ratios early in the growing season (prior to August 1), led to increased water infiltration in grassland soils. Water infiltration was positively associated with increased litter mass under adaptive grazing, whereas higher bulk density (and sandier) soils were associated with decreased infiltration rates. This study highlights the potential of specialised rotational grazing systems using cattle to improve soil hydrologic function in grazed grasslands.

How land-use and soil depth affect soil organic carbon (SOC) quality and stability, properties that are key to the long-term storage of C, is poorly understood in agroforestry systems. We examined the effects of land-use (forest vs. annual cropland) and soil depth (0–10 vs. 10–30 cm) on the quality (availability of C for decomposition), structural stability (composition of C functional groups), and thermal stability (energy yield and temperature characteristics during thermal oxidation) of SOC in a hedgerow system in central Alberta, using solid-state 13C nuclear magnetic resonance (NMR) spectroscopy and thermal analysis methods. The SOC quality (proportion of O-alkyl C) was higher, while the proportion of H- and C-substituted aromatic C and structural stability (hydrophobicity index (HB/HI), aromaticity index (ARM), and alkyl index (A/OA)) were all lower in the forest than in the cropland. The SOC also had lower thermal stability (higher energy density and lower temperature at which 50% of the mass was lost (TG-T50)) in the forest than in the cropland. Between the two soil depths, SOC quality (proportion of N-alkyl C, O-alkyl C and di-O-alkyl C) was higher, but the proportion of H- and C-substituted aromatic C and structural and thermal stabilities were lower, within the surficial 0–10 cm layer than in the 10–30 cm soil. We conclude that while the forest land-use within the hedgerow system had a higher SOC quality, it had lower SOC structural and thermal stabilities, suggesting that SOC under the forest land-use may be more susceptible to decline under climate change or other forms of anthropogenic disturbance.

Livestock grazing affects plant community composition, diversity, and carbon (C) and nutrient cycling in grasslands. Grazing leads to plant communities that have higher relative abundance of grazing-tolerant species, which in turn may alter the chemical composition of biomass and subsequent litter decomposition rates. To better understand the effects of long-term grazing and associated vegetation shifts on biogeochemical cycling in northern temperate grasslands of western Canada, we studied litter decomposition over 18 months at 15 locations, stratified across the Mixed-grass Prairie, Central Parkland, and Foothills Fescue natural subregions. At each location, we examined decomposition in an area exposed to grazing and an area where cattle were excluded. We used litterbags containing leaf litter from seven major grass species representing different grazing tolerances and included a local source of community litter from each study site and cellulose paper as standards. Decomposition was affected by litter types, with litter from grazing-tolerant species such as Poa pratensis and Bouteloua gracilis having faster decomposition rates compared to grazing-intolerant species, supporting the hypothesis that changes in vegetation composition due to grazing influences biogeochemical cycling by modifying litter decomposition in grasslands. Litter decomposition was also overall most rapid in the cool–wet Foothills Fescue, followed by the temperate mesic Central Parkland, and slowest in the warmer–drier Mixed-grass Prairie. Combined with known grazing-induced changes in grassland composition, these findings indicate that livestock grazing may accelerate litter decomposition rates in the more mesic Foothills Fescue and parkland regions, but not the more arid Mixed-grass Prairie. Overall, this study elucidates the role of livestock grazing and its associated effects on litter decomposition and ecosystem processes in northern grassland ecosystems.

Grasslands cover more than 40% of the terrestrial surface of Earth and provide a range of ecological goods and services, including serving as one of the largest reservoirs for terrestrial carbon. An understanding of how livestock grazing, influences grassland soil organic carbon (SOC), including its concentration, vertical distribution and association among soil-particle sizes is unclear. We quantified SOC concentrations in the upper 30 cm of mineral soil, together with SOC particle-size association, within 108 pairs of long-term grazed and non-grazed grassland study sites spanning six distinct climate subregions across a 5.7 M ha area of Alberta, Canada. Moderate grazing enhanced SOC concentration by 12% in the upper 15 cm of soil. Moreover, SOC concentrations in mineral layers were associated with regional climate, such that SOC increased from dry to mesic subregions. Our results also indicate that C concentrations in each of 2000–250, 250–53

Livestock grazing is an important driver of the carbon (C) cycle in grasslands and could be a decisive factor determining whether grasslands are a net sink or source of C. However, short-term C dynamics in grassland ecosystems and its response to different grazing intensities remain unclear, especially under field conditions. Here we test the hypothesis that photosynthetic C allocation in the plant-soil system of grasslands is differentially affected by the intensity of grazing that occurred in the prior growing season. We studied the fate of new C assimilates, using a 13CO2 pulse-labeling technique, as they move from shoots to roots and soil in vegetation previously exposed to different defoliation (emulated grazing) intensities in two semiarid Mixed Prairie grassland ecosites having contrasting soil textures (sandy vs. loamy). Within 24 h after photosynthetic uptake, 34.5% of the labeled C was translocated out of shoots, with 21% of the labeled C found in the mineral soil. Plant communities treated with high intensity defoliation during the previous growing season had more of the newly fixed 13C translocate into live roots at both ecosites throughout the 25-day chase period, indicating a higher demand for photosynthetic C by the root system of heavily defoliated plants. Twenty-five days after labeling, 13C transfer into the top 30 cm mineral soil was consistently greater at the loamy (26% of total 13C remaining) than at the sandy ecosite (10.4% of 13C remaining). We conclude that conditioning with high intensity defoliation the prior growing season stimulated short-term C allocation to roots in these semi-arid grasslands. Although this response was independent of soil texture, more C was translocated belowground overall in the loamy ecosite. The effect of defoliation intensity and ecosite in regulating short-term C allocation in the plant-soil system in temperate grasslands should be incorporated into future models describing C cycling in grasslands when predicting C dynamics under different management strategies employing contrasting grazing intensities.

Rotational stocking theoretically enables regrazing of regrowth, but regrowth may be limited in xerophytic vegetation. We tested the hypothesis that regrowth would be slow and fixed in Hesperostipa comata (a caespitose, drought-tolerant grass), while growth would be flexible in Pascopyrum smithii (a rhizomatous, less drought-tolerant grass) under increasing defoliation and moisture, by assessing tiller growth rates, population dynamics and plant yield on marked plants in a Dry Mixed Grass prairie. Plots were clipped in late summer to simulate a deferred control, or intermittently during the growing season (May–August) at high intensity–low frequency (HILF), low intensity–high frequency (LIHF) or high intensity–high frequency and crossed with two water treatments (ambient and addition) in both a xeric upland and a mesic lowland. Growing season defoliation increased tiller growth rates of P. smithii in the upland, whereas HILF and LIHF reduced growth in the lowland. All defoliation regimes increased tiller growth for H. comata. Tiller populations of H. comata increased with frequent defoliation, while tiller populations of P. smithii decreased regardless of defoliation regime. Frequent defoliation, regardless of intensity, reduced yield relative to the deferred control for both grasses. While water addition consistently increased growth rates and reduced differences in tiller number among defoliation treatments, the regrowth of both grasses remained similar under varied defoliation with ambient moisture. High moisture conditions also promoted regrowth more in P. smithii compared to H. comata. In summary, neither grass species displayed an inherently fixed or flexible tiller or plant yield response in response to defoliation or moisture.