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David Bergman,1 Bjorn Roelstraete,1 Jiangwei Sun,1 Fahim Ebrahimi,1,2 Rickard Lidström,3 Axel Svedbom,4,5 Mona Ståhle,4 Jonas F Ludvigsson1,6,7 1Department of Medical Epidemiology and Biostatistics, Karolinska Institutet, Stockholm, Sweden; 2Department of Gastroenterology and Hepatology, Clarunis University Center for Gastrointestinal and Liver Diseases, Basel, Switzerland; 3Diagnostiskt Centrum Hud, Stockholm, Sweden; 4Division of Dermatology and Venereology, Department of Medicine, Karolinska Institutet, Stockholm, Sweden; 5Dermatology and Venereology Clinic, Karolinska University Hospital, Stockholm, Sweden; 6Department of Pediatrics, Orebro University Hospital, Orebro, Sweden; 7Department of Medicine, Columbia University College of Physicians and Surgeons, New York, NY, USACorrespondence: David Bergman, Email David.bergman.1@ki.seBackground: Microscopic colitis (MC) has been associated with several immune-mediated diseases including psoriasis, but earlier research has been limited to psoriasis occurring before MC. Data from large-scale cohort studies investigating MC and risk of future psoriasis are lacking.Objective: To examine the association between MC and psoriasis.Methods: In a nationwide, population-based, matched cohort study in Sweden from 2007 to 2021, we identified 8404 patients with biopsy-verified MC (diagnosed in 2007– 2017), 37,033 matched reference individuals, and 8381 siblings without MC. Information on MC was obtained through the ESPRESSO cohort (a Swedish histopathology database with nationwide coverage). Using Cox regression, we calculated hazard ratios (HRs) and 95% confidence intervals (CIs) for psoriasis up until 2021.Results: During a median follow-up of 9.2 years (interquartile range = 6.7– 11.7), 179 MC patients and 440 reference individuals were diagnosed with psoriasis (241.1 vs 131.8 events per 100,000 person-years), corresponding to one extra case of psoriasis in 91 patients with MC over 10 years. After adjustment for the matching variables (birth year, sex, county of residence, and calendar period) and level of education, we computed an adjusted hazard ratio (aHR) of 1.82 (95% CI = 1.53– 2.17). Stratified by sex, estimates were similar and when examining the aHR across different lengths of follow-up, we found significantly elevated estimates up to 10 years after MC diagnosis. Compared to MC-free siblings, the aHR was 1.85 (95% CI = 1.36– 2.51).Conclusion: Patients with MC are at an almost doubled risk of psoriasis compared to the general population. Clinicians need to consider psoriasis in MC patients with skin lesions.Keywords: microscopic colitis, psoriasis, epidemiology

Fatemeh Ebrahimi,1,2 Amirhossein Sahebkar,3–5 Naheed Aryaeian,2 Naseh Pahlavani,6 Soudabeh Fallah,7 Nariman Moradi,8 Davoud Abbasi,9 Agha Fatemeh Hosseini10 1Skull Base Research Center, Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 2Department of Nutrition, School of Public Health, Iran University of Medical Sciences, Tehran, Iran; 3Halal Research Center of IRI, FDA, Tehran, Iran; 4Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; 5Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; 6Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran; 7Department of Clinical Biochemistry, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran; 8Department of Clinical Biochemistry, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran; 9Department of Internal Medicine, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran; 10Department of Biostatistics, School of Public Health, Iran University of Medical Sciences, Tehran, IranCorrespondence: Naheed AryaeianDepartment of Nutrition, School of Public Health, Iran University of Medical Sciences, Shahid Hemmat Expressway, Iran University of Medical Sciences, Hemat Highway next to Milad Towe Tehran, Tehran 1449614535, IranTel +98-912-3093353Fax +98-21-8862253Email Aryaeian.n@iums.ac.irBackground: Type 2 diabetes (T2D) is accompanied by elevated inflammation, oxidative stress, hyperlipidemia and hyperglycemia which all contribute to cardiovascular disease pathogenesis. Saffron as a complementary medicine and source of antioxidants could play a role in alleviating diabetes and its complications. The aim of this study was to determine the effects of saffron supplementation as an adjunct therapy in T2D.Patients and methods: This randomized controlled trial included 80 T2D patients with a mean age of 54.1 years. Participants were randomly assigned into two groups to take either saffron tablets (100 mg/day; n=40) or placebo (n=40) for 12 weeks. Fasting blood samples were obtained at the beginning and after the intervention period to quantify glycemic factors, lipid profile, and biomarkers of inflammation and oxidative stress. Anthropometric indices and dietary intakes were also measured at baseline and at study end.Results: Compared with placebo, saffron supplementation resulted in significant decreases in waist circumference (p0.05).Conclusion: Overall, 12 weeks of saffron supplementation in diabetic patients had beneficial effects on waist circumference and serum MDA levels. However, saffron did not influence other evaluated cardio metabolic risk markers in diabetic patients.Keywords: crocus sativus, diabetes mellitus, type 2, dysglycemia, lipid profile, inflammation mediators

Background and Aim: Enterohemorrhagic Escherichia coli (EHEC) cause a wide spectrum of infections, such as diarrhea, hemorrhagic colitis and hemolytic–uremic syndrome. Considering the risks associated with antibiotic therapy against EHEC infection, vaccines can be a promising method for prevention of infections. Recombinant chimeric proteins containing multiple immunogens could induce immunity against bacterial infections. The aim of this study was to evaluate immunogenecity of trivalent chimeric antigen EspA -Stx2b -Intimin against E.coli O157 H7 infection. Material and Methods: In this descriptive-laboratory study,recombinant chimeric protein was expressed in E.coli BL21 DE3 by use of IPTG. The protein expression was evaluated by SDS-PAGE and western blotting analysis. The recombinant protein was purified using Ni–NTA affinity chromatography. The immunization was conducted in mice with purified protein and antibody titers were determined by ELISA. Following immunization, mice were infected with E.coli O157:H7 and evaluated for bacterial shedding and mortality. Using SPSS software, statistical analysis was performed by Duncan's test and T-test. Results: The protein was expressed in E.coli BL21 (DE3) and SDS-PAGE analysis showed expression of recombinant protein with molecular weight of 63kD. Western blot analysis confirmed presence of chimeric protein. ELISA results showed that immunogenic properties of chimeric protein induced humoral response to EspA, intimin and Stx2b. Bacterial shedding in immunized mice decreased to 102 cfu/ml and mortality rate was reduced to 60%. Conclusion: The results showed that the chimeric protein induced humoral response and protected the mice against E.coli O157:H7.

Introduction. Multilevel inverters (MLIs) are a popular industrial and, more especially, renewable energy application solution. This is because of its appetite for filters, low distortion class, and capacity to provide a multilayer output voltage that resembles a pure sine waveform. The novelty is in applying the wild horse optimization algorithm (WHOA) to adjust the sinusoidal pulse width modulation (SPWM) technique by producing the optimal reference signal parameters in a new multilevel inverter architecture known as the packed U-cell multilevel inverter (PUC-MLI). Purpose. This study helps with the idea of new inverter architecture and a modified pulse width modulation (MPWM) method to make the multilevel inverter smaller, cheaper, and with less total harmonic distortion (THD). Methods. We use the proposed approach to control a 7-level, single-phase PUC-MLI. The WHOA is used to discover the optimal parameters of the additional reference sine signal after being compared with SPWM to evaluate its performance in harmonic reduction. The simulation’s outcome was validated by building a PUC-MLI prototype. Results. Experimental results and simulations validate the effectiveness of the suggested approach. The WHOA-improved MPWM approach achieves a significant reduction in THD on the PUCMLI output voltage, as indicated by the results. Practical value. THD in MLI output voltage will be reduced without spending any cost. The suggested solution works with many MLI topologies with varying output voltage levels. References 20, tables 6, figures 12. [ABSTRACT FROM AUTHOR]

The National Spherical Torus Experiment (NSTX) has undergone a major upgrade, and the NSTX Upgrade (NSTX-U) Project was completed in the summer of 2015. NSTX-U first plasma was subsequently achieved, diagnostic and control systems have been commissioned, the H-mode accessed, magnetic error fields identified and mitigated, and the first physics research campaign carried out. During ten run weeks of operation, NSTX-U surpassed NSTX record pulse-durations and toroidal fields (TF), and high-performance ∼1 MA H-mode plasmas comparable to the best of NSTX have been sustained near and slightly above the n = 1 no-wall stability limit and with H-mode confinement multiplier H98y,2 above 1. Transport and turbulence studies in L-mode plasmas have identified the coexistence of at least two ion-gyro-scale turbulent micro-instabilities near the same radial location but propagating in opposite (i.e. ion and electron diamagnetic) directions. These modes have the characteristics of ion-temperature gradient and micro-tearing modes, respectively, and the role of these modes in contributing to thermal transport is under active investigation. The new second more tangential neutral beam injection was observed to significantly modify the stability of two types of Alfven eigenmodes. Improvements in offline disruption forecasting were made in the areas of identification of rotating MHD modes and other macroscopic instabilities using the disruption event characterization and forecasting code. Lastly, the materials analysis and particle probe was utilized on NSTX-U for the first time and enabled assessments of the correlation between boronized wall conditions and plasma performance. These and other highlights from the first run campaign of NSTX-U are described.

The National Spherical Torus Experiment (NSTX) is currently being upgraded to operate at twice the toroidal field and plasma current (up to 1T and 2MA), with a second, more tangentially aimed neutral beam (NB) for current and rotation control, allowing for pulse lengths up to 5s. Recent NSTX physics analyses have addressed topics that will allow NSTX-Upgrade to achieve the research goals critical to a Fusion Nuclear Science Facility. These include producing stable, 100% non-inductive operation in high-performance plasmas, assessing plasma-material interface (PMI) solutions to handle the high heat loads expected in the next-step devices and exploring the unique spherical torus (ST) parameter regimes to advance predictive capability. Non-inductive operation and current profile control in NSTX-U will be facilitated by co-axial helicity injection (CHI) as well as radio frequency (RF) and NB heating. CHI studies using NIMROD indicate that the reconnection process is consistent with the 2D Sweet-Parker theory. Full-wave AORSA simulations show that RF power losses in the scrape-off layer (SOL) increase significantly for both NSTX and NSTX-U when the launched waves propagate in the SOL. Toroidal Alfvén eigenmode avalanches and higher frequency Alfvén eigenmodes can affect NB-driven current through energy loss and redistribution of fast ions. The inclusion of rotation and kinetic resonances, which depend on collisionality, is necessary for predicting experimental stability thresholds of fast growing ideal wall and resistive wall modes. Neutral beams and neoclassical toroidal viscosity generated from applied 3D fields can be used as actuators to produce rotation profiles optimized for global stability. DEGAS-2 has been used to study the dependence of gas penetration on SOL temperatures and densities for the MGI system being implemented on the Upgrade for disruption mitigation. PMI studies have focused on the effect of ELMs and 3D fields on plasma detachment and heat flux handling. Simulations indicate that snowflake and impurity seeded radiative divertors are candidates for heat flux mitigation in NSTX-U. Studies of lithium evaporation on graphite surfaces indicate that lithium increases oxygen surface concentrations on graphite, and deuterium-oxygen affinity, which increases deuterium pumping and reduces recycling. In situ and test-stand experiments of lithiated graphite and molybdenum indicate temperature-enhanced sputtering, although that test-stand studies also show the potential for heat flux reduction through lithium vapour shielding. Non-linear gyro kinetic simulations have indicated that ion transport can be enhanced by a shear-flow instability, and that non-local effects are necessary to explain the observed rapid changes in plasma turbulence. Predictive simulations have shown agreement between a microtearing-based reduced transport model and the measured electron temperatures in a microtearing unstable regime. Two Alfvén eigenmode-driven fast ion transport models have been developed and successfully benchmarked against NSTX data. Upgrade construction is moving on schedule with initial physics research operation of NSTX-U planned for mid-2015.