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3 results on '"ENHANCED AFFINITY"'

1. Photo-induced protonation assisted nano primary battery for highly efficient immobilization of diverse heavy metal ions.

Author

Ge, Hongjian, Chen, Chaowen, Li, Sijia, Guo, Xinyue, Zhang, Jing, Yang, Pengqi, Xu, Huan, Zhang, Jia, and Wu, Zhengyan

Abstract

Highly-stable heavy metal ions (HMIs) appear long-term damage, while the existing remediation strategies struggle to effectively remove a variety of oppositely charged HMIs without releasing toxic substances. Here we construct an iron-copper primary battery-based nanocomposite, with photo-induced protonation effect, for effectively consolidating broad-spectrum HMIs. In FCPBN, Fe/Cu cell acts as the reaction impetus, and functional graphene oxide modified by carboxyl and UV-induced protonated 2-nitrobenzaldehyde serves as an auxiliary platform. Due to the groups and built-in electric fields under UV stimuli, FCPBN exhibits excellent affinity for ions, with a maximum adsorption rate constant of 974.26 g∙mg-1∙min-1 and facilitated electrons transfer, assisting to reduce 9 HMIs including Cr 2 O 7 2-, AsO 2 -, Cd2+ in water from 0.03 to 3.89 ppb. The cost-efficiency, stability and collectability of the FCPBN during remediation, and the beneficial effects on polluted soil and the beings further demonstrate the splendid remediation performance without secondary pollution. This work is expected to remove multi-HMIs thoroughly and sustainably, which tackles an environmental application challenge. [Display omitted] ● Different electrical heavy metal ions could be simultaneously remediated to ppb level. ● Showing almost no leakage of pollutants after remediation. ● The economy of consuming less time and money. ● Good remediation effect on polluted-soil obtained from Chizhou, China. [ABSTRACT FROM AUTHOR]

Published
2024
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2. Estrogen receptor-KRAB chimeras are potent ligand-dependent repressors of estrogen-regulated gene expression

Subjects
ZINC-FINGER PROTEINS, ENHANCED AFFINITY, RESPONSE ELEMENT, PS2 GENE, RNA-POLYMERASE-II, BREAST-CANCER, HORMONE-INDEPENDENT TRANSACTIVATION, TRANSCRIPTION ACTIVATION, hormones, hormone substitutes, and hormone antagonists, IN-VIVO, NUCLEAR RECEPTORS
Abstract

As an approach to targeted repression of genes of interest, we describe the development of human estrogen receptor (ER) alpha-KRAB repressor domain chimeras that are potent ligand-dependent repressors of the transcription of estrogen response element (ERE)-containing promoters and analyze their mechanisms of action. Repression by the KRAB domain was dominant over transactivation mediated by ER AF1 and AF2. An ERE and an ER ligand (estrogen or antiestrogen) were required for repression. Studies with several promoters and cell lines demonstrated that the presence of EREs, rather than the capacity for estrogen induction, determines the potential for repression of a gene by the KRAB-ER alpha-KRAB (HERK) chimera. A single consensus ERE was sufficient for repression, but the KERK chimera was unable to suppress transcription from the imperfect ERE in the native pS2 promoter. We recently reported mutations that enhance binding of a steroid receptor DNA-binding domain to the ERE. Introducing these mutations into wild-type ER enhanced transactivation from the pS2 ERE. Insertion of these mutations into KERK created the novel repressor KERK-3M, which is a potent repressor of both ER-induced and basal transcription on a promoter containing the pS2 ERE. These modified ER-KRAB chimeras should prove useful as new tools for the functional analysis and repression of ER-regulated genes.

Published
2000

3. Estrogen receptor-KRAB chimeras are potent ligand-dependent repressors of estrogen-regulated gene expression

Author

de Haan, G, Chusacultanachai, S, Mao, CJ, Katzenellenbogen, BS, Shapiro, DJ, University of Groningen, and Stem Cell Aging Leukemia and Lymphoma (SALL)

Subjects
ZINC-FINGER PROTEINS, ENHANCED AFFINITY, RESPONSE ELEMENT, PS2 GENE, RNA-POLYMERASE-II, BREAST-CANCER, HORMONE-INDEPENDENT TRANSACTIVATION, TRANSCRIPTION ACTIVATION, hormones, hormone substitutes, and hormone antagonists, IN-VIVO, NUCLEAR RECEPTORS
Abstract

As an approach to targeted repression of genes of interest, we describe the development of human estrogen receptor (ER) alpha-KRAB repressor domain chimeras that are potent ligand-dependent repressors of the transcription of estrogen response element (ERE)-containing promoters and analyze their mechanisms of action. Repression by the KRAB domain was dominant over transactivation mediated by ER AF1 and AF2. An ERE and an ER ligand (estrogen or antiestrogen) were required for repression. Studies with several promoters and cell lines demonstrated that the presence of EREs, rather than the capacity for estrogen induction, determines the potential for repression of a gene by the KRAB-ER alpha-KRAB (HERK) chimera. A single consensus ERE was sufficient for repression, but the KERK chimera was unable to suppress transcription from the imperfect ERE in the native pS2 promoter. We recently reported mutations that enhance binding of a steroid receptor DNA-binding domain to the ERE. Introducing these mutations into wild-type ER enhanced transactivation from the pS2 ERE. Insertion of these mutations into KERK created the novel repressor KERK-3M, which is a potent repressor of both ER-induced and basal transcription on a promoter containing the pS2 ERE. These modified ER-KRAB chimeras should prove useful as new tools for the functional analysis and repression of ER-regulated genes.

Published
2000

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