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Avian embryos have been at the core of embryological, morphological, physiological and biochemical/molecular research, especially involving research in three primary areas: developmental, biomedical and agricultural research. As developmental models, the avian embryo—especially that of the chicken—has been the single most used embryo model, perhaps in part from the combination of large size, ease of access and prior knowledge base. Developmental research with avian embryos has included organ system studies of the heart, vasculature, lungs, kidneys, nervous system, etc., as well as integrated physiological processes including gas-exchange, acid–base and ion/water regulation. In terms of translational research, avian embryos have modelled vascular development, based on the easily accessible chorioallantoic membrane under the eggshell. This same respiratory organ has enabled toxicological studies of how pollutants affect vertebrate development. Investigation of the transition to pulmonary breathing and the associated emergence of respiratory control has also relied heavily upon the avian embryo. In addition to developmental and biomedical investigations, the avian embryo has been studied intensively due to the huge importance of domesticated birds as a food source. Consequently, the effects of environment (including temperature, humidity, noise levels and photoperiod) during incubation on subsequent post-hatch phenotype are being actively investigated. This article is part of the theme issue 'The biology of the avian respiratory system'. [ABSTRACT FROM AUTHOR]

Background: Sperm DNA fragmentation is an important factor that affects male fertility. This study intends to evaluate the impact of sperm DNA damage [single-strand breaks (SSB) and double-strand breaks (DSB)] on fertilisation and embryonic euploidy after intracytoplasmic sperm injection (ICSI). Of the different sperm selection techniques, the novel microfluidic sperm sorting (MSS) ZyMōt™ ICSI device reduces both SSB and DSB in semen samples. The second objective is to study the impact of this MSS device on fertilisation and embryonic euploidy. Materials and Methods: This retrospective study included data from 167 ICSI treatments. The alkaline and neutral Comet assays were used to analyse SSB and DSB, respectively. Analysis of fertilisation and embryo euploidy rates was performed in four groups of patients with normal/altered SSB or DSB values. Density gradient centrifugation (DGC) and the ZyMōt™ ICSI MSS device were used for semen preparation. Results: Fertilisation rates in 167 ICSI cycles were higher when using sperm from patients with normal SSB values (70.84%) and patients with abnormal SSB whose samples were processed using the MSS device (69.71%) compared to patients with abnormal SSB values using DGC (58.49%). Preimplantation genetic testing for aneuploidies (PGT-A) revealed a higher embryo euploidy rate in patients with normal DSB values (60.00%) and patients with abnormal DSB whose samples were processed using the MSS device (44.59%) compared to patients with abnormal DSB values using DGC (36.84%). When female age was considered, there were fewer euploid embryos in women ≥35 years of age compared to younger women, independently of SSB and DSB. The number of euploid embryos increased when the MSS device was used. Conclusion: High SSB and DSB values in semen samples decreased fertilisation rates and embryonic euploidy, respectively. The ZyMōt™ ICSI device for semen preparation increased both rates, especially in couples that included women <35 years old. [ABSTRACT FROM AUTHOR]

Chickens are sensitive to environmental challenges caused by temperature. The current study aimed to determine the effects of heat manipulation during embryonic development on the physiological responses of Goliath chickens. A total of 2000 hatching eggs from 48-week-old breeders were weighed, numbered, and randomly distributed equally into 4 incubators. Each incubator received 500 eggs (4 replicates of 125 eggs each). Eggs in two of the incubators were rotated hourly at a 45° angle and maintained at 37.8°C and 60% relative humidity (T0 groups). Between embryonic days (ED) 10 and 18 of incubation, the eggs from the other two incubators were heated to 38.5°C for 6 hours per day (T1 groups). The eggs were reweighed and candled, and viable eggs were moved to the hatching baskets at ED 18 of incubation. Hatching eggs were examined individually for hatching events every three hours during the final three days of incubation. On day 21, blood samples were collected from 12 chicks per group for hormonal and biochemical analyses. The evaluated blood parameters included Triiodothyronine (T3), T4 (thyroxine), cortisol, uric acid, lactate dehydrogenase, and total protein. At hatch, chicks were weighed and their quality (survival after hatching and performance standards) was evaluated. Data were collected on embryonic development, hatching window, hatching events, biochemical parameters, and hormonal concentrations. Results indicated that hatchability, chick's weight, Triiodothyronine, and corticosterone were higher in the T1 group, compared to the control group. At hatch on day 21, the pipping muscle of chicks in the treated group (T1) was significantly heavier than that of the control group, while the embryonic mortality rate was significantly higher in the T0 group. In conclusion, applying heat treatment for 6 hours at 38.5°C from ED10-ED18 of embryogenesis increased significantly the hatching rate, the pipping muscle, and the chick's weight in this study. [ABSTRACT FROM AUTHOR]

Normal reproductive function and fertility is considered a "sixth vital sign" because disruptions to this sensitive physiological system can forewarn other health issues, including exposure to environmental toxicants. We found that female mice exhibited profound loss of embryos during pre-implantation and fetal development coincident with a change to the source of their drinking water. When female mice were provided with tap water from the building in which they were housed (Water 2), instead of tap water from a neighboring building which was their previous supply (Water 1), ovulated oocytes were degenerated or had impaired meiotic maturation, and failed to form embryos. The harmful effects of Water 2 exposure were not reversible even following a recovery period; however, carbon-filtration of Water 2 removed the toxic contaminant. Water composition analysis to identify the responsible toxicant(s) found that trace elements were present at expected levels and phthalates were undetectable. Per- and Poly-fluoroalkyl Substances (PFAS), a family of persistent organic pollutants were detected at ~4 ng/L. To investigate further, female mice were given drinking water categorized by level of PFAS contamination (0.6 ng/L, 2.8 ng/L, or 4.4 ng/L) for 9 weeks. Compared to mice consuming purified MilliQ water, mice consuming PFAS-contaminated water had decreased oocyte quality, impaired embryogenesis and reduced cell numbers in blastocysts. PFAS concentration in the drinking water was negatively correlated with oocyte viability. Importantly, the levels of PFAS detected in the tap water are within current "safe level" guidelines, and further research is needed to determine whether PFAS are responsible for the observed reproductive toxicity. However, this research demonstrating that water deemed suitable for human consumption has detrimental effects on mammalian embryo development has important implications for public health and water quality policies. [ABSTRACT FROM AUTHOR]

Only 30% of embryos from in vitro fertilized oocytes successfully implant and develop to term, leading to repeated transfer cycles. To reduce time-to-pregnancy and stress for patients, there is a need for a diagnostic tool to better select embryos and oocytes based on their physiology. The current standard employs brightfield imaging, which provides limited physiological information. Here, we introduce METAPHOR: Metabolic Evaluation through Phasor-based Hyperspectral Imaging and Organelle Recognition. This non-invasive, label-free imaging method combines two-photon illumination and AI to deliver the metabolic profile of embryos and oocytes based on intrinsic autofluorescence signals. We used it to classify i) mouse blastocysts cultured under standard conditions or with depletion of selected metabolites (glucose, pyruvate, lactate); and ii) oocytes from young and old mouse females, or in vitro-aged oocytes. The imaging process was safe for blastocysts and oocytes. The METAPHOR classification of control vs. metabolites-depleted embryos reached an area under the ROC curve (AUC) of 93.7%, compared to 51% achieved for human grading using brightfield imaging. The binary classification of young vs. old/in vitro-aged oocytes and their blastulation prediction using METAPHOR reached an AUC of 96.2% and 82.2%, respectively. Finally, organelle recognition and segmentation based on the flavin adenine dinucleotide signal revealed that quantification of mitochondria size and distribution can be used as a biomarker to classify oocytes and embryos. The performance and safety of the method highlight the accuracy of noninvasive metabolic imaging as a complementary approach to evaluate oocytes and embryos based on their physiology. [ABSTRACT FROM AUTHOR]

Background: Selecting embryos with the highest implantation potential is crucial for in vitro fertilization (IVF) success. Both the timing of blastulation, day 5 (D5) or D6, and the embryo quality have been suggested as influential factors in determining the clinical outcome of single euploid blastocyst transfers. However, evidence supporting the superiority of D5 over D6 blastocysts remains inconclusive. The aim of this study was to compare clinical outcomes following the transfer of euploid blastocysts with different quality and timing of blastulation. Materials and Methods: A retrospective cohort study was conducted at our Assisted Reproductive Center, analyzing the outcome of 774 transfers with D5 euploids and 155 transfers with D6 euploids performed between January 2019 and February 2022. Results: The live birth rate was significantly lower in the euploid D6 group compared to the euploid D5 group (38.71vs. 55.04%, P=0.001). The outcome was significantly influenced by the quality of the embryos. Live birth rates were 62.14 and 53.61% following transfers of D5 and D6 excellent embryos respectively, 45.18 and 32.21% following transfer of D5 and D6 good embryos but only 28.64 and 19.32% following transfer of D5 and D6 fair embryos. The outcome difference was statistically significant across embryo quality categories (P=0.001). The adjusted risk ratios (RR) of clinical outcomes indicated that excellent euploid D5 embryos consistently outperformed other types of embryo quality. Conclusion: The timing of blastulation and embryo quality are crucial factors in determining the success of single euploid blastocyst transfers. Excellent euploid D5 transfers yielded superior clinical outcomes, providing valuable insights for IVF teams and patients when selecting embryos to be transferred. [ABSTRACT FROM AUTHOR]

Preimplantation embryo culture, pivotal in assisted reproductive technology (ART), has lagged in innovation compared to embryo selection advancements. This review examines the persisting gap between in vivo and in vitro embryo development, emphasizing the need for improved culture conditions. While in humans this gap is hardly estimated, animal models, particularly bovines, reveal clear disparities in developmental competence, cryotolerance, pregnancy and live birth rates between in vitro-produced (IVP) and in vivo-derived (IVD) embryos. Molecular analyses unveil distinct differences in morphology, metabolism, and genomic stability, underscoring the need for refining culture conditions for better ART outcomes. To this end, a deeper comprehension of oviduct physiology and embryo transport is crucial for grasping embryo–maternal interactions' mechanisms. Research on autocrine and paracrine factors, and extracellular vesicles in embryo–maternal tract interactions, elucidates vital communication networks for successful implantation and pregnancy. In vitro, confinement, and embryo density are key factors to boost embryo development. Advanced dynamic culture systems mimicking fluid mechanical stimulation in the oviduct, through vibration, tilting, and microfluidic methods, and the use of innovative softer substrates, hold promise for optimizing in vitro embryo development. [ABSTRACT FROM AUTHOR]

Parthenogenesis is a form of asexual reproduction in which a gamete (ovum or sperm) develops without being fertilized. Tomer Jordi Chaffer uses parthenogenesis to challenge Don Marquis' future‐like‐ours (FLO) argument against abortion. According to Marquis, (1) what makes it morally wrong to kill us is that it would deprive us of a possible future that we might come to value—a future "like ours" (FLO) and (2) human fetuses are numerically identical to any adult human organism they may develop into, and thus have a FLO. Chaffer contends that if human ova are capable of parthenogenesis, then they would have a FLO, which contraception may deprive them of, but contends this is absurd. Bruce P. Blackshaw challenges Chaffer, contending sexually fertilized embryos are not identical to unfertilized ovum, but this would yield a more absurd implication, that fertilization deprives an ovum of a FLO! Here I show Marquis' account of identity rules out both Chaffer's and Blackshaw's accounts. [ABSTRACT FROM AUTHOR]

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Vertebrate limbs start to develop as paired protrusions from the lateral plate mesoderm at specific locations of the body with forelimb buds developing anteriorly and hindlimb buds posteriorly. During the initiation process, limb progenitor cells maintain active proliferation to form protrusions and start to express Fgf10 , which triggers molecular processes for outgrowth and patterning. Although both processes occur in both types of limbs, forelimbs (Tbx5), and hindlimbs (Isl1) utilize distinct transcriptional systems to trigger their development. Here, we report that Sall1 and Sall4 , zinc finger transcription factor genes, regulate hindlimb initiation in mouse embryos. Compared to the 100% frequency loss of hindlimb buds in TCre; Isl1 conditional knockouts, Hoxb6Cre; Isl1 conditional knockout causes a hypomorphic phenotype with only approximately 5% of mutants lacking the hindlimb. Our previous study of SALL4 ChIP-seq showed SALL4 enrichment in an Isl1 enhancer, suggesting that SALL4 acts upstream of Isl1. Removing 1 allele of Sall4 from the hypomorphic Hoxb6Cre; Isl1 mutant background caused loss of hindlimbs, but removing both alleles caused an even higher frequency of loss of hindlimbs, suggesting a genetic interaction between Sall4 and Isl1. Furthermore, TCre -mediated conditional double knockouts of Sall1 and Sall4 displayed a loss of expression of hindlimb progenitor markers (Isl1, Pitx1, Tbx4) and failed to develop hindlimbs, demonstrating functional redundancy between Sall1 and Sall4. Our data provides genetic evidence that Sall1 and Sall4 act as master regulators of hindlimb initiation. [ABSTRACT FROM AUTHOR]

Most in vitro models of oviduct epithelial cells (OEC) used thus far to gain insights into embryo–maternal communication induce cell dedifferentiation or are technically challenging. Moreover, although the presence of developing embryos has been shown to alter gene expression in OEC, the effect of embryos on OEC physiology remains largely unknown. Here, we propose a model based on bovine oviduct epithelial spheroids (OES) with specific shape and diameter (100–200 μm) criteria. The aims of this study were to i) determine the appropriate culture conditions of bovine OES cultured in suspension by evaluating their morphology, total cell number, viability, and activity of ciliated cells; ii) monitor gene expression in OES at the time of their formation (day 0) and over the 10 days of culture; and iii) test whether the vicinity of developing embryos affects OES quality criteria. On day 10, the proportions of vesicle-shaped OES (V-OES) were higher in M199/500 (500 μl of HEPES-buffered TCM-199) and synthetic oviduct fluid (SOF)/25 (25-μL droplet of SOF medium under mineral oil) than in M199/25 (25-μL droplet of M199 under mineral oil). The proportion of viable cells in V-OES was not affected by culture conditions and remained high (>80%) through day 10. The total number of cells per V-OES decreased over time except in SOF/25, while the proportions of ciliated cells increased over time in M199/500 but decreased in M199/25 and SOF/25. The movement amplitude of OES in suspension decreased over time under all culture conditions. Moreover, the gene expression of ANXA1 , ESR1 , HSPA8 , and HSPA1A in OES remained stable during culture, while that of PGR and OVGP1 decreased from day 0 to day 10. Last, the co-culture of developing embryos with OES in SOF/25 increased the rates of blastocysts on days 7 and 8 compared to embryos cultured alone, and increased the proportion of V-OES compared to OES cultured alone. In conclusion, M199/500 and SOF/25 provided the optimal conditions for the long-time culture of OES. The supporting effect of OES on embryo development and of developing embryos on OES morphology was evidenced for the first time. Altogether, these results point OES as an easy-to-use, standardizable, and physiological model to study embryo–maternal interactions in cattle. • Oviduct epithelial spheroids (OES) were obtained from isthmic mucosa fragments. • Large volume of M199 and droplets of SOF offered good culture conditions for OES. • OES expressed oviduct epithelial cell markers during 10 days in culture. • OES supported embryo development in SOF medium. • Embryo supported OES morphology in SOF medium. [ABSTRACT FROM AUTHOR]

The presence of per- and polyfluoroalkyl substances (PFASs) in aquatic environments is often persistent and widespread. Understanding the potential adverse effects from this group of chemicals on aquatic communities allows for better hazard characterization. This study examines impacts on zebrafish (Danio rerio) embryo physiology, behavior, and lipid levels from exposure to perfluorooctanoic acid (PFOA), perfluorohexane sulfonate (PFHxS), and heptadecafluorooctanesulfonic acid (PFOS). Embryos were exposed to lethal and sublethal levels of each chemical and monitored for alterations in physiological malformations, mortality, lipid levels, and behavior (only PFOA and PFHxS). The predicted 50% lethal concentrations for 120 hpf embryos were 528.6 ppm PFOA, 14.28 ppm PFHxS, and 2.14 ppm PFOS. Spine curvature and the inability of the 120 hpf embryos to maintain a dorsal-up orientation was significantly increased at 10.2 ppm PFHxS and 1.9 ppm PFOS exposure. All measured 120 hpf embryo behaviors were significantly altered starting at the lowest levels tested, 188 ppm PFOA and 6.4 ppm PFHxS. Lipid levels decreased at the highest PFAS levels tested (375 PFOA ppm, 14.4 PFHxS ppm, 2.42 ppm PFOS). In general, the PFAS chemicals, at the levels examined in this study, increased morphological deformities, embryo activity, and startle response time, as well as decreased lipid levels in 120 hpf zebrafish embryos. [ABSTRACT FROM AUTHOR]

Introduction: MicroRNAs are small noncoding genes with gene expression regulatory function. Their emergence as potential diagnostic biomarker for many diseases has gained a specific interest among researchers. Observations of changes in miRNA levels correlating with aneuploidy in early embryos raise the prospective of employing miRNA as biomarkers to assess the embryo quality. Method: To identify and gather the miRNAs with potential link to chromosomal abnormalities in embryos from previous research, we conducted a systematic search using four databases, including Embase, Medline, Web of Science, and Cochrane databases in accordance with PRISMA guidelines. Results: Out of 200 identified records, only seven met the inclusion criteria. Seven miRNAs: miR-19b, miR-517c, miR-518e, miR-522, miR-92a, and miR-106a exhibited persistent downregulation in aneuploid blastocysts in the included studies. These miRNAs are members of important miRNA clusters, associated with abnormal expression in studies on reproductive failure. Pathway analysis revealed their involvement in regulating gene transcription, as well as cell cycle progression and apoptosis. Discussion: The changes detected in the miRNA expression in aneuploid embryos across different studies support the aneuploidy and miRNA relationship and prospect miRNA as a valuable tool for the assessment of embryo quality. Collectively, these observations highlight the role of miRNAs in embryonic development, and their involvement in genetic abnormalities that occur in embryos, such as aneuploidy, indicating their potential implementation to improve the embryo selection and reproductive outcomes. [ABSTRACT FROM AUTHOR]

In the last four decades, the assisted reproductive technology (ART) field has witnessed advances, resulting in improving pregnancy rates and diminishing complications, in particular reduced incidence of multiple births. These improvements are secondary to advanced knowledge on embryonic physiology and metabolism, resulting in the ability to design new and improved culture conditions. Indeed, the incubator represents only a surrogate of the oviduct and uterus, and the culture conditions are only imitating the physiological environment of the female reproductive tract. In vivo, the embryo travels through a dynamic and changing environment from the oviduct to the uterus, while in vitro, the embryo is cultured in a static fashion. Importantly, while culture media play a critical role in optimising embryo development, a large host of additional factors are equally important. Additional potential variables, including but not limited to pH, temperature, osmolality, gas concentrations and light exposure need to be carefully controlled to prevent stress and permit optimal implantation potential. This manuscript will provide an overview of how different current culture conditions may affect oocyte and embryo viability with particular focus on human literature. [ABSTRACT FROM AUTHOR]

Pro-inflammatory cytokines may contribute to physiological, e.g., tissue reconstruction and vascularization, or pathological, e.g., inflammation or some metabolic disturbances, processes during pregnancy establishment. The current study aimed to investigate IL-6 plasma levels at 30 and 45 days post-insemination (dpi) in pregnant and non-pregnant dairy cows. The cows were examined for pregnancy diagnosis at 30 and 45 dpi. Cows (n=71) were assigned into four groups: Non-pregnant at 30 dpi (n=16), pregnant at 30 dpi (n=36), non-pregnant at 45 dpi (n=11), and pregnant at 45 dpi (n=8). Blood samples were collected at 30 and 45 dpi for IL-6 assay. Results showed high levels of plasma IL-6 at 30 dpi either cow-conceived or not. Cows with healthy embryos at 45 dpi had lower concentrations of IL-6 than cows with late embryonic death. The plasma IL-6 concentrations showed different patterns of changes in dairy cows during the first two months of pregnancy. [ABSTRACT FROM AUTHOR]

High resolution assessment of cardiac functional parameters is crucial in translational animal research. The chick embryo is a historically well‐used in vivo model for cardiovascular research due to its many practical advantages, and the conserved form and function of the chick and human cardiogenesis programs. This review aims to provide an overview of several different technical approaches for chick embryo cardiac assessment. Doppler echocardiography, optical coherence tomography, micromagnetic resonance imaging, microparticle image velocimetry, real‐time pressure monitoring, and associated issues with the techniques will be discussed. Alongside this discussion, we also highlight recent advances in cardiac function measurements in chick embryos. Key Findings: Studying the physiology of the chick embryo is critical to understand many diseases such as CHDs.Advancements have been made in techniques to measure the blood flow velocity such as using Doppler Electrocardiography, Optical Coherence Tomography and micro‐MRI.Measurement of Blood pressure is equally important and techniques have been developed to measure the blood pressure through direct measurement and through servo null micropressure technique. [ABSTRACT FROM AUTHOR]

The article discusses a study by Daniel Gorelick and Vahid Zadmajid on how androgens influence development through membrane-associated signaling pathways in zebrafish embryos. The researchers identified GPRC6A as a key mediator of non-genomic androgen signaling, leading to cardiac edema and tail abnormalities. The study sheds light on previously unknown mechanisms of androgen signaling and its impact on cardiovascular development in zebrafish embryos. The researchers plan to further explore membrane steroid receptors and their roles in normal development and disease progression. [Extracted from the article]

As a woody grass, bamboo has special reproductive habits with an extremely long vegetative phase. Pleioblastus pygmaeus is a kind of ornamental dwarf bamboo species with high ecological and economic value in China. There was no documentary record of the flowering of P. pygmaeus until 2015, which bloomed in the Bamboo Garden of Nanjing Forestry University. Generally, bamboo plants rarely bear fruit or have a low seed setting rate, but this species has a high seed production. This study explores developmental reasons using anatomical methods. The process of flower bud differentiation and a series of important reproductive development processes including anther wall development, microsporogenesis, megasporogenesis, male and female gametophyte development, and embryonic development were investigated sequentially. Each stamen contained three tetrasporangiate anthers and the development of anther wall was of Monocotyledonous type. The microspores finally matured into 3-celled pollen grains by two successive mitoses. The pistil was composed of three carpels, with an anatropous, tenuinucellate and bitegmic ovule in a single ovary. The formation of embryo sac belonged to Polygonum type. With three mitoses, functional megaspore developed into the embryo sac with eight nuclei within seven cells. The development of stamen and pistil was synchronous in the same floret. The development of embryo followed Grass type and the development of endosperm belonged to Nuclear type. The development of endosperm was earlier than that of embryo. The study enriches basic knowledge of reproductive biology of bamboo plants and is of great fundamental significance to furtherly explore flowering mechanism of P. pygmaeus and to cultivate bamboo plants sustainably. [ABSTRACT FROM AUTHOR]

Whereas both sperm and egg contribute nuclear genetic material to the zygote in metazoan organisms, the inheritance of other cellular constituents is unequal between the 2 gametes. Thus, 2 copies of the centriole are contributed solely by the sperm to the zygote in most species. Centrioles can have a stereotyped distribution in some asymmetric divisions, but whether sperm-contributed centrioles are distributed in a stereotyped manner in the resulting embryo is not known. Here, we address this question in Caenorhabditis elegans using marked mating experiments, whereby the presence of the 2 sperm-contributed centrioles is monitored in the embryo using the stable centriolar component SAS-4::GFP, as well as GFP::SAS-7. Our analysis demonstrates that the distribution of sperm-contributed centrioles is stochastic in 4-cell stage embryos. Moreover, using sperm from zyg-1 mutant males that harbor a single centriole, we show that the older sperm-contributed centriole is likewise distributed stochastically in the resulting embryo. Overall, we conclude that, in contrast to the situation during some asymmetric cell divisions, centrioles contributed by the male germ line are distributed stochastically in embryos of C. elegans. [ABSTRACT FROM AUTHOR]

Many internal (inherent) and environmental (imposed) factors control seed dormancy and germination that we divide into three basic dormancy‐release pathways: Maternal structures and embryo physiology control inherent dormancy that is broken by various types of scarification and physiological changes, followed by imposed‐dormancy release when the prevailing environment is replaced by certain 'standard' conditions that stimulate germination (Pathway 1); imposed dormancy prevails even if inherent dormancy is broken or not applicable that is released when replaced by certain 'standard' environmental conditions which stimulate germination (Pathway 2); and release from inherent dormancy by light/dark or cold stratification is contingent on the pre‐existence of certain environmental conditions that stimulate germination (Pathway 3).On‐plant seed storage (serotiny) and frugivorous seeds are recognized here as representing special types of physical dormancy, as their properties are consistent with those of hard diaspores. Warm stratification does not require seeds to be moist as it is just a physical response. Heat may promote germination of non‐hard, as well as hard, seeds as it may increase their permeability further.Levels of germination gauge the net effect of inherent‐ and imposed‐dormancy release so that it is only possible to identify the extent of inherent‐dormancy release when conditions for germination are optimal (imposed dormancy has been annulled). While imposed dormancy may be protracted after inherent dormancy is broken by heat or chilling during the dry or cold seasons, release from both states may effectively coincide if smoke chemicals or light are received during the (wet) growing season.We suggest reserving the term secondary dormancy for seeds that return to (inherent or imposed) dormancy due to changed environmental conditions. Under seasonal climates, fluctuations in environmental conditions can lead to secondary dormancy and even dormancy cycling.We recognize four types of functional interactions between any two environmental factors that induce inherent‐dormancy release: binary interactions are ineffective, only one stimulus is effective, both are effective but non‐additive, or both are additive/synergistic. Two environmental stimuli that individually break dormancy but have no additive effect must be affecting the same process; this was demonstrated here for some interactions between heat and smoke.The three dormancy‐release pathways, together with internal, seasonal and stochastic interactions, are coordinated by the non‐dormant seed to ensure maximum germination under optimal conditions. To ignore any aspect outlined here leads to an impoverished understanding of the disparate seed ecology of species adapted to different stressful and disturbance‐prone habitats. Read the free Plain Language Summary for this article on the Journal blog. [ABSTRACT FROM AUTHOR]

While omics has transformed the study of biology, concomitant advances made at the level of the whole organism, i.e. the phenome, have arguably not kept pace with lower levels of biological organisation. In this personal commentary we evaluate the importance of imaging as a means of measuring whole organismal developmental physiology. Image acquisition, while an important process itself, has become secondary to image analysis as a bottleneck to the use of imaging in research. Here, we explore the significant potential for increasingly sophisticated approaches to image analysis, including deep learning, to advance our understanding of how developing animals grow and function. Furthermore, unlike many species-specific methodologies, tools and technologies, we explore how computer vision has the potential to be transferable between species, life stages, experiments and even taxa in which embryonic development can be imaged. We identify what we consider are six of the key challenges and opportunities in the application of computer vision to developmental physiology carried out in our lab, and more generally. We reflect on the tangibility of transferrable computer vision models capable of measuring the integrative physiology of a broad range of developing organisms, and thereby driving the adoption of phenomics for developmental physiology. We are at an exciting time of witnessing the move from computer vision as a replacement for manual observation, or manual image analysis, to it enabling a fundamentally more powerful approach to exploring and understanding the complex biology of developing organisms, the quantification of which has long posed a challenge to researchers. [Display omitted] • Computer vision is driving exciting advances in whole-organismal developmental biology. • We offer a personal perspective on the present and future of imaging in this field. • Approaches such as deep learning are driving greater transferability. • We consider what transferrable image analysis tools might look like, and enable, in future. [ABSTRACT FROM AUTHOR]

Dr. John F. Hasler, a distinguished figure in reproductive physiology and embryo transfer, has been awarded the 2025 Inaugural IETS Distinguished Practitioner Award. With a career spanning decades, Dr. Hasler co-founded Em Tran Inc., a pioneering company in commercial embryo transfer, and made significant contributions to the field through research and industry standards. His dedication to the industry, support for young practitioners, and numerous accolades highlight his deserving recognition as the recipient of this prestigious award. [Extracted from the article]

Clotilde Cadart, a researcher in developmental biology, discusses her career path and research interests in an interview. She initially pursued medicine but became interested in science during a program for medical students. Her research focuses on cell size and division, as well as cell size and metabolism in frogs. She found that cell size affects metabolic rate in embryos. Cadart emphasizes the importance of interdisciplinary research and the challenges of being an outsider in multiple fields. She also served as the President of the Postdoctoral Association at Berkeley. In addition to her research, Cadart enjoys outdoor activities like swimming and hiking. [Extracted from the article]

BACKGROUND: Perfluoroalkyl acids (PFAA) have been measured in ovarian follicular fluid from women using in vitro fertilization (IVF), although associations between follicular fluid PFAA and IVF outcomes have been inconsistent. OBJECTIVES: We investigated the association between follicular fluid PFAA and embryo quality in women undergoing IVF. METHODS: We prospectively enrolled 729 women undergoing IVF treatment in Guangxi province, China, from July 2018 to December 2018. We measured 32 PFAA, including branched isomers, in follicular fluid using ultra-performance liquid chromatography coupled to tandem mass spectrometry. We applied restricted cubic splines, linear regression, and log-binominal regression models to investigate associations between follicular fluid PFAA and embryo quality, adjusting for confounding variables and investigated oocyte maturity as an intervening variable using causal mediation analysis. We further estimated the overall effect of the PFAA mixture on outcomes using Bayesian kernel machine regression (BKMR). RESULTS: We detected 8 of 32 measured PFAA in >85% of follicular fluid samples. Higher PFAA concentrations were associated with fewer high-quality embryos from IVF. The high-quality embryo rates at the 50th percentile of linear perfluoro-1-octanesulfonate acid (n-PFOS), all branched PFOS isomers (Br-PFOS) and linear perfluoro-n-octanoic acid (n-PFOA) were -6.34% [95% confidence interval (CI): -9.45, -3.32%], -16.78% (95% CI: -21.98, -11.58%) and -8.66% (95% CI: -11.88, -5.43%) lower, respectively, than the high quality embryo rates at the reference 10th percentile of PFAA. Oocyte maturity mediated 11.76% (95% CI: 3.18, 31.80%) and 14.28% (95% CI: 2.95, 31.27%) of the n-PFOS and n-PFOA associations, respectively. The results of the BKMR models showed a negative association between the PFAA mixture and the probability of high-quality embryos, with branched PFOS isomers having posterior inclusion probabilities of 1 and accounting for the majority of the association. DISCUSSION: Exposure to higher PFAA concentrations in follicular fluid was associated with poorer embryo quality during IVF. Branched PFOS isomers may have a stronger effect than linear PFOS isomers. More studies are needed to confirm these findings and to directly estimate the effects on pregnancy and live-birth outcomes. [ABSTRACT FROM AUTHOR]

Vertebrate embryos require access to water; however, many species nest in terrestrial habitats that vary considerably in moisture content. Oviparous, non‐avian reptiles have served as models to understand how environmental factors, like moisture availability, influence development because eggs are often exposed to prevailing environments in the absence of parental care. Though much research demonstrates the importance of water absorption by eggs, many ecological factors that influence moisture availability in natural nests have received little attention. For example, the type of substrate in which nests are constructed is understudied. We experimentally incubated eggs of the brown anole lizard (Anolis sagrei) in 2 naturally occurring nest substrates that were treated with varying amounts of water to determine how natural substrates influence development at different moisture concentrations. One substrate consisted of sand and crushed seashells and the other was mostly organic material (i.e. decayed plant material). Both are common nesting substrates at our field site. When controlling for water uptake by eggs, we found that egg survival and hatchling phenotypes were similar between substrates; however, embryos developed more quickly in the sand/shell substrate than the organic substrate, indicating substrate‐specific effects on embryo physiology. These results demonstrate that different natural substrates can result in similar developmental outcomes if the water available to eggs is comparable; however, some aspects of development, like developmental rate, are affected by the type of substrate, independent of water availability. Further study is required to determine how natural substrates influence embryo physiology independent of water content. [ABSTRACT FROM AUTHOR]

The chicken hatching egg is a self-contained life-supporting system for the developing embryo. However, the post-hatch performance of birds depends on several factors, including the breeder management and age, egg storage conditions and duration before incubation, and the incubation conditions. Studies have determined the effect of incubation factors on chick post-hatch growth potential. Therefore, chick physical quality at hatch is receiving increasing attention. Indeed, although incubation temperature, humidity, turning and ventilation are widely investigated, the effects of several variables such as exposure of the embryo to high or low levels, time of exposure, the amplitude of variations and stage exposures on embryo development and post-hatch performance remain poorly understood. This review paper focuses on chick quality and post-hatch performance as affected by incubation conditions. Also, chick physical quality parameters are discussed in the context of the parameters for determining chick quality and the factors that may affect it. These include incubation factors such as relative humidity, temperature, turning requirements, ventilation, in ovo feeding and delay in feed access. All these factors affect chick embryo physiology and development trajectory and consequently the quality of the hatched chicks and post-hatch performance. The potential application of adapted incubation conditions for improvement of post-hatch performance up to slaughter age is also discussed. It is concluded that incubation conditions affect embryo parameters and consequently post-hatch growth differentially according to exposure time and stage of exposure. Therefore, classical physical conditions are required to improve hatchability, chick quality and post-hatch growth. [ABSTRACT FROM AUTHOR]

Objective: To investigate Sonic hedgehog (Shh) effects on proliferation and apoptosis of tongue epithelial cells in embryonic and ageing mice. Materials and Methods: Embryonic day 13.5 (E13.5), E14.5, E16.5 and postnatal day 0.5 (PN0.5) K14‐Cre;Shhfl/fl mice, and E14.5, E16.5, PN0.5, PN90.5 and postnatal 1.5 years (PN1.5Y) wild‐type (Wt) mice were employed. Scanning electron microscopy, haematoxylin–eosin and immunohistochemistry were performed. Gel beads containing exogenous Shh protein were embedded in the tongue of PN90.5 and PN1.5Y Wt mice. Three days later, 5‐bromodeoxyuridine (BrdU), proliferating cell nuclear antigen (PCNA) immunohistochemical and TUNEL staining were performed. Results: The number of fungiform papillae was decreased with age. The numbers of BrdU‐ and PCNA‐positive cells were the highest at PN0.5 and the lowest at PN1.5Y. Compared with Wt mice, K14‐Cre;Shhfl/fl mice had decreased PCNA‐positive cells in the epithelium, a smaller tongue volume, and fewer papillae at PN0.5. At E14.5, the number of BrdU‐positive cells was decreased in K14‐Cre;Shh fl/fl mice. At PN1.5Y, the number of apoptotic cells in tongue tissue exposed to Shh protein was less than that in the BSA group and the numbers of BrdU‐ and PCNA‐positive proliferating cells were increased. Conclusion: Shh maintains cell proliferation and reduces apoptosis during tongue development and ageing. [ABSTRACT FROM AUTHOR]

The ubiquitin-mediated degradation of oocyte translational regulatory proteins is a conserved feature of the oocyte-to-embryo transition. In the nematode Caenorhabditis elegans, multiple translational regulatory proteins, including the TRIM-NHL RNA-binding protein LIN-41/Trim71 and the Pumilio-family RNA-binding proteins PUF-3 and PUF-11, are degraded during the oocyte-to-embryo transition. Degradation of each protein requires activation of the M-phase cyclin-dependent kinase CDK-1, is largely complete by the end of the first meiotic division and does not require the anaphase-promoting complex. However, only LIN-41 degradation requires the F-box protein SEL-10/FBW7/Cdc4p, the substrate recognition subunit of an SCF-type E3 ubiquitin ligase. This finding suggests that PUF-3 and PUF-11, which localize to LIN-41-containing ribonucleoprotein particles, are independently degraded through the action of other factors and that the oocyte ribonucleoprotein particles are disassembled in a concerted fashion during the oocyte-to-embryo transition. We develop and test the hypothesis that PUF-3 and PUF-11 are targeted for degradation by the proteasome-associated HECT-type ubiquitin ligase ETC-1/UBE3C/Hul5, which is broadly expressed in C. elegans. We find that several GFP-tagged fusion proteins that are degraded during the oocyte-to-embryo transition, including fusions with PUF-3, PUF-11, LIN-41, IFY-1/Securin, and CYB-1/Cyclin B, are incompletely degraded when ETC-1 function is compromised. However, it is the fused GFP moiety that appears to be the critical determinant of this proteolysis defect. These findings are consistent with a conserved role for ETC-1 in promoting proteasome processivity and suggest that proteasomal processivity is an important element of the oocyte-to-embryo transition during which many key oocyte regulatory proteins are rapidly targeted for degradation. [ABSTRACT FROM AUTHOR]

Photobiomodulation therapy (PBMT) has been widely used to promote tissue repair. However, PBMT's critical roles in the epithelial and mesenchymal tissues interactions are still barely known. Herein, we investigated light parameters on challenged keratinocytes (KC)—i.e., cultivated under oxidative stress—solely or associated with fibroblasts (FB) in a co-culture system. Cells were treated with PBMT at the wavelength of 660 nm, at 20 mW and 0.71 W/cm2 . Three different energy densities were primarily evaluated on KC: 1 (1.4 s), 5 (7 s), and 50 J/cm² (70 s). Next, KC and FB were co-cultured and assessed at 5 J/cm². This energy density was also tested in ex vivo murine skin samples. Our main data suggest that PBMT can increase cellular proliferation at low doses and cell migration in a biphasic mode (1 and 50 J/cm² ), both further confirmed by the epidermal growth factor receptor ligand—amphiregulin—upregulation. IL-1RA mRNA—the IL-1β (interleukin-1β) receptor antagonist recognized to fasten wound repair—was upregulated in the co-culture system. Upon PBMT, the ex vivo findings showed a progressive increase in the epidermal thickness, although presenting qualitatively less differentiated epithelium than the control group. In conclusion, PBMT effects are dependent on the cellular interactions with the surrounding microenvironment. Ultimately, PBMT is antiinflammatory and contributes to the expression of critical mediators of wound repair. [ABSTRACT FROM AUTHOR]

Diverse lines of evidence indicate that the mammalian oviduct makes important contributions to the complex process of reproduction other than being simply a conduit for the transport of gametes and embryos. The cumulative synthesis and transport of proteins secreted by oviductal secretory cells into the oviductal lumen create a microenvironment supporting important reproductive events, including sperm capacitation, fertilization, and early embryo development. Among the components that have been identified in the oviductal fluid is a family of glycosylated proteins known collectively as oviduct-specific glycoprotein (OVGP1) or oviductin. OVGP1 has been identified in several mammalian species, including humans. The present review summarizes the work carried out, in various mammalian species, by many research groups revealing the synthesis and secretion of OVGP1, its fate in the female reproductive tract upon secretion by the oviductal epithelium, and its role in modulating biological functions of gametes and embryos. The production and functions of recombinant human OVGP1 and recombinant OVGP1 of other mammalian species are also discussed. Some of the findings obtained with immunocytochemistry will be highlighted in the present review. It is hoped that the findings obtained from recent studies carried out with recombinant OVGP1 from various species will rekindle researchers' interest in pursuing further the role of the oviductal microenvironment, of which OVGP1 is a major component, in contributing to the successful occurrence of early reproductive events, and the potential use of OVGP1 in improving the current assisted reproductive technology in alleviating infertility. [ABSTRACT FROM AUTHOR]

During the first 20 weeks of pregnancy especially, fetuses occasionally raise their hands and place them behind the head; sometimes they even cross their legs and lie as though "relaxing in a hammock".8 Fetal Motility Clusters in Rest and Activity Cycles By 20 weeks' gestation, fetal activity shows a diurnal variation with peaks in the late evening. Interestingly, both general and breathing movements are unrelated within pairs of twins, which suggests that the motor development of twins is largely independent of their co-twins' development.23 An adequate intrathoracic space and amount of amniotic fluid as well as normal fetal breathing movements are required for lung development. 2b), can already be observed during pregnancy.19 GLO:1VN/01oct21:dmcn14918-fig-0002.jpg PHOTO (COLOR): 2 Fetal limb movement patterns recorded by means of dynamic magnetic resonance imaging: (a) grasping the umbilical cord (27wks' gestation); (b) extended index finger (27wks' gestation); (c) alternating leg movements (19wks' gestation).8 gl Isolated leg movements are less frequent than isolated arm movements but emerge at the same time (10-11wks' gestation), which indicates that motor system development does not progress in a craniocaudal sequence. Isolated finger movements emerge at around 13 weeks' gestation; cloni and twitches appear after 14 weeks, but are rare even then.8,12 During the third trimester, the fetus manipulates and even squeezes the umbilical cord (Fig. [Extracted from the article]

Improved reproductive performance in buffaloes can be achieved by understanding the basic mechanism governing the embryonic attachment and feto-maternal communication. Considering this, trascriptomic profiling and integrative analysis of long intergenic non-coding RNAs were carried out in the uterine caruncles of pregnant and non-pregnant buffaloes. Transcriptome data of pregnant and non-pregnant uterine caruncles after quality control was used to perform the analysis. Total of 86 novel lincRNAs expressed in uterine caruncular tissues were identified and characterized. Differential expression analysis revealed that 447 mRNAs and 185 mRNAs were up- and down- regulated, respectively. The number of up- and down- regulated lincRNAs were 114 and 13, respectively. Of the identified 86 novel lincRNAs, six novel lincRNAs were up-regulated in the pregnant uterine caruncles. GO terms (biological process) and PANTHER pathways associated with reproduction and embryogenesis were over-represented in differentially expressed genes. Through miRNA interaction analysis, interactions of 16 differentially expressed lincRNAs with mi-RNAs involved in reproduction were identified. This study has provided a catalogue of differentially expressed genes and novel regions previously unknown to play a significant role in buffalo reproduction. The results from the current study extends the buffalo uterine lncRNAs database and provides candidate regulators for future molecular genetic studies on buffalo uterine physiology to improve the embryo implantation and successful completion of pregnancy. • Catalogue novel regions previously unknown to play a significant role in buffalo reproduction were identified. • Total of 86 novel lincRNAs expressed in uterine caruncular tissues were identified and characterized • GO terms (biological process) and PANTHER pathways associated with reproduction and embryogenesis were over-represented for the differentially expressed genes • Interaction of differentially expressed lincRNAs with mi-RNAs involved in reproduction were identified • Regulatory motifs identified in top differential expressed genes were found to be associated with GO terms for reproduction and embryogenesis [ABSTRACT FROM AUTHOR]

Background: Polycystic ovary syndrome (PCOS) is considered to be one of the most common endocrine disorders in women of reproductive age. Zinc, a vital trace element in the body, plays a key role in maintaining health, especially due to its antioxidant role. On the other hand, lack of antioxidants and oxidative stress can adversely affect oocytes quality and consequently fertility rate. The available studies that report the effect of follicular fluid (FF) zinc in terms of the number and quality of the oocytes in infertile women with PCOS, are few and not consistent. We decided to investigate this issue. Materials and Methods: In this cross-sectional study, from the women with PCOS referring to Omolbanin Hospital, Dezful, Iran (February to December 2019), a total of 90 samples (follicular fluid, oocytes, and embryos) were collected from those who had undergone in vitro fertilization (IVF). To measure zinc level in follicular fluid, high performance liquid chromatograpy (HPLC) was utilized. Also, oocytes maturity and embryos quality evaluation was performed using inverted optical microscopy. One-way ANOVA and Fisher's least significant difference (LSD) were used for data analysis. Results: The amount of FF zinc was not associated with any significant differences in the number of oocytes and metaphase I (MI) and germinal vesicle (GV) oocytes, but a significant decrease was observed in the number of metaphase II (MII) oocytes at zinc values less than 35 µg/dL. The FF zinc levels less than 35 µg/dL were also significantly associated with decreased embryo quality. Conclusion: A significant relationship was found between the level of FF zinc and the quality and the number of oocytes taken from the ovaries of infertile patients with PCOS history who were candidates for IVF treatment as well as the number of high quality embryos. [ABSTRACT FROM AUTHOR]

The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor well-known for its adaptive role as a sensor of environmental toxicants and mediator of the metabolic detoxification of xenobiotic ligands. In addition, a growing body of experimental data has provided indisputable evidence that the AHR regulates critical functions of cell physiology and embryonic development. Recent studies have shown that the naïve AHR—that is, unliganded to xenobiotics but activated endogenously—has a crucial role in maintenance of embryonic stem cell pluripotency, tissue repair, and regulation of cancer stem cell stemness. Depending on the cellular context, AHR silences the expression of pluripotency genes Oct4 and Nanog and potentiates differentiation, whereas curtailing cellular plasticity and stemness. In these processes, AHR-mediated contextual responses and outcomes are dictated by changes of interacting partners in signaling pathways, gene networks, and cell-type-specific genomic structures. In this review, we focus on AHR-mediated changes of genomic architecture as an emerging mechanism for the AHR to regulate gene expression at the transcriptional level. Collective evidence places this receptor as a physiological hub connecting multiple biological processes whose disruption impacts on embryonic development, tissue repair, and maintenance or loss of stemness. [ABSTRACT FROM AUTHOR]

Humans are changing and challenging nature in many ways. Conservation Biology seeks to limit human impacts on nature and preserve biological diversity. Traditionally, Developmental Biology and Conservation Biology have had nonoverlapping objectives, operating in distinct spheres of biological science. However, this chasm can and should be filled to help combat the emerging challenges of the 21st century. The means by which to accomplish this goal were already established within the conceptual framework of evo‐ and eco‐devo and can be further expanded to address the ways that anthropogenic disturbance affect embryonic development. Herein, I describe ways that these approaches can be used to advance the study of reptilian embryos. More specifically, I explore the ways that a developmental perspective can advance ongoing studies of embryonic physiology in the context of global warming and chemical pollution, both of which are known stressors of reptilian embryos. I emphasize ways that these developmental perspectives can inform conservation biologists trying to develop management practices that will address the complexity of challenges facing reptilian embryos. Research Highlights: Reptiles and their embryos are under threat of emerging anthropogenic change such as pollution and thermal stress. Herein, I outline areas of future research where greater information about reptilian development in the context of real‐world challenges may inform conservation efforts. [ABSTRACT FROM AUTHOR]

The first third of incubation is critical for embryonic development, and environmental changes during this phase can affect the physiology and survival of the embryos. We evaluated the effects of low (LT), control (CT), and high (HT) temperatures during the first 5 days of incubation on ventilation (V. E ), body temperature (Tb), oxygen consumption (V. O2), respiratory equivalent (V. E / V. O2), and brain monoamines on 3-days-old (3d) and 14-days-old (14d) male and female chickens. The body mass of LT animals of both ages and sexes was higher compared to HT and CT animals (except for 3d males). The heart mass of 14d HT animals was higher than that of CT animals. Thermal manipulation did not affect V. E , V. O2 or V. E / V. O2 of 3d animals in normoxia, except for 3d LT males V. E , which was lower than CT. Regarding 14d animals, the HT females showed a decrease in V. E and V. O2 compared to CT and LT groups, while the HT males displayed a lower V. O2 compared to CT males, but no changes in V. E / V. O2. Both sexes of 14d HT chickens presented a greater Tb compared to CT animals. Thermal manipulations increased the dopamine turnover in the brainstem of 3d females. No differences were observed in ventilatory and metabolic parameters in the 3d animals of either sexes, and 14d males under 7% CO2. The hypercapnic hyperventilation was attenuated in the 14d HT females due to changes in V. O2, without alterations in V. E . The 14d LT males showed a lower V. E , during hypercapnia, compared to CT, without changes in V. O2, resulting in an attenuation in V. E / V. O2. During hypoxia, 3d LT females showed an attenuated hyperventilation, modulated by a higher V. O2. In 14d LT and HT females, the increase in V. E was greater and the hypometabolic response was attenuated, compared to CT females, which resulted in no change in the V. E / V. O2. In conclusion, thermal manipulations affect hypercapnia-induced hyperventilation more so than hypoxic challenge, and at both ages, females are more affected by thermal manipulation than males. [ABSTRACT FROM AUTHOR]

Objective: To compare the rates of blastocyst stage development between embryos fertilized after one (MPN) or more than two pronucleus (PN) (3PN, 4PN-multiPN) with those after 2PN in the same patients. Material and Methods: The embryos of patients who had both abnormal PN (MPN, 3PN or 4PN) and normal fertilized (2PN) embryos after intracytoplasmic sperm injection (ICSI) fertilization, were followed with a time-lapse system following the ICSI procedure. The rates of reaching the blastocyst stage were compared between normal and abnormally fertilized embryos. Results: One thousand eight hundred and twenty oocytes were collected from 140 patients and 1280 (70.3%) of them were fertilized. MPN, 2PN and 3PN, 4PN (multiPN) ratios of the embryos in the pronuclear stage were 11.4%, 83.13% and 5.47%, respectively. The rates of reaching the blastocyst stage among these embryos were 17.1%, 60.8% and 42.8% for MPN, 2PN and multiPN, respectively. The proportion reaching blastocyst development was significantly higher following 2PN compared to those after MPN and multiPN (p<0.05). Embryos developing after multiPN had significantly higher rates of reaching the blastocyst stage compared to those after MPN (p<0.01). Conclusion: The majority of abnormally pronucleated embryos arrest without reaching the blastocyst stage. MultiPN embryos have a higher rate of blastocyst development than MPN embryos. [ABSTRACT FROM AUTHOR]

The present study was conducted to decipher the proteome of in vivo‐produced pre‐implantation ovine embryos. Ten locally adapted Morana Nova ewes received hormonal treatment and were inseminated 12 hr after ovulation. Six days later, 54 embryos (morula and blastocyst developmental state) were recovered from eight ewes and pooled to obtain sufficient protein for proteomic analysis. Extracted embryo proteins were analysed by LC‐MS/MS, followed by identification based on four database searches (PEAKS, Proteome Discoverer software, SearchGUI software, PepExplorer). Identified proteins were analysed for gene ontology terms, protein clusters and interactions. Genes associated with the ovine embryo proteome were screened for miRNA targets using data sets of TargetScan (http://www.targetscan.org) and mIRBase (http://www.mirbase.org) servers. There were 667 proteins identified in the ovine embryos. Biological processes of such proteins were mainly related to cellular process and regulation, and molecular functions, to binding and catalytic activity. Analysis of the embryo proteins revealed 49 enriched functional clusters, linked to energy metabolism (TCA cycle, pyruvate and glycolysis metabolism), zona pellucida (ZP), MAPK signalling pathway, tight junction, binding of sperm to ZP, translation, proteasome, cell cycle and calcium/phospholipid binding. Sixteen miRNAs were related to 25 pre‐implantation ovine embryo genes, all conserved in human, bovine and ovine species. The interaction network generated by miRNet showed four key miRNAs (hsa‐mir‐106b‐5p; hsa‐mir‐30‐5p; hsa‐mir‐103a‐5p and hsa‐mir‐106a‐5p) with potential interactions with embryo‐expressed genes. Functional analysis of the network indicated that miRNAs modulate genes related to cell cycle, regulation of stem cell and embryonic cell differentiation, among others. Retrieved miRNAs also modulate the expression of genes involved in cell signalling pathways, such as MAPK, Wnt, TGF‐beta, p53 and Toll‐like receptor. The current study describes the first major proteomic profile of 6‐day‐old ovine embryos produced in vivo, setting a comprehensive foundation for our understanding of embryo physiology in the ovine species. [ABSTRACT FROM AUTHOR]

Disorders affecting the sperm, oocyte, or embryo may cause a significant fraction of spontaneous miscarriages and cases of recurrent pregnancy loss (RPL). Altered chromosomal integrity of sperm and oocytes, which is highly dependent of the age of the mother, represents a major cause of miscarriage and in turn RPL. Avoiding transfers of abnormal embryos is possible with preimplantation genetic testing for aneuploidies. Chromosomal anomalies may also be caused by structural rearrangements of one or several chromosomes in either parents, a finding encountered in 12% of couples with RPL, including in those who have had one or several healthy babies. More than 40% of these chromosomal rearrangements are identifiable on regular karyotypes. When abnormal findings are made, preimplantation genetic testing for monogenic disorders allows selection of disease-free embryos. Finally, asymmetric inactivation of the X chromosome has been found more commonly in women with RPL, but no specific treatment is currently available. [ABSTRACT FROM AUTHOR]

Objective: To evaluate the effect of embryo stage at transfer on placental histopathology and perinatal outcome in singleton live births resulting from fresh embryo transfers (ETs).Design: Retrospective cohort study.Setting: Not applicable.Patient(s): The study population included all live births after fresh ETs during the period from 2009 to 2017.Intervention(s): None.Main Outcome Measure(s): Primary outcomes included anatomic, inflammatory, vascular malperfusion, and villous maturation placental features. Secondary outcomes included fetal, maternal, and perinatal complications.Result(s): A total of 677 live births were included in the final analysis and were allocated to the cleavage-stage (n = 252) and blastocyst (n = 425) ET groups. After the adjustment for confounding factors, the blastocyst group was found to be associated with a higher incidence of circummarginate membranes insertion (odds ratio [OR] 1.9, 95% confidence interval [CI] 1.2-3.4), delayed villous maturation (OR 8.5, 95% CI 1.2-69.3), chorangiosis (OR 2.0, 95% CI 1.2-3.8), parenchymal calcifications (OR 10.6, 95% CI 1.4-80.2), and intrapartum nonreassuring fetal heart rate tracing (OR 2.4, 95% CI 1.3-4.5). Compared with cleavage-stage ETs, live births resulting from the blastocysts were associated with a lower incidence of velamentous cord insertion (OR 0.5, 95% CI 0.3-0.9), retroplacental hematoma (OR 0.3, 95% CI 0.1-0.8), subchorionic thrombi (OR 0.3, 95% CI 0.1-0.8), and avascular villi (OR 0.2, 95% CI 0.03-0.7).Conclusion(s): Live births resulting from fresh cleavage-stage and blastocyst ETs have different placental histopathology features, with a higher rate of intrapartum nonreassuring fetal heart rate tracing in the blastocyst group. [ABSTRACT FROM AUTHOR]

The focus of this paper is to review best practices for rapid-cooling cryopreservation of oocytes and embryos. The discussion of best practices includes the types of cryoprotectants and cryo devices typically used. Key performance indicators of rapid-cooling vitrification success are defined. [ABSTRACT FROM AUTHOR]

Aspects of global change result in warming temperatures that threaten biodiversity across the planet. Eggs of non‐avian, oviparous reptiles (henceforth "reptiles") are particularly vulnerable to warming due to a lack of parental care during incubation and limited ability to behaviorally thermoregulate. Because warming temperatures will cause increases in both mean and variance of nest temperatures, it is crucial to consider embryo responses to both chronic and acute heat stress. Although many studies have considered embryo survival across constant incubation temperatures (i.e., chronic stress) and in response to brief exposure to extreme temperatures (i.e., acute stress), there are no standard metrics or terminology for determining heat stress of embryos. This impedes comparisons across studies and species and hinders our ability to predict how species will respond to global change. In this review, we compare various methods that have been used to assess embryonic heat tolerance in reptiles and provide new terminology and metrics for quantifying embryo responses to both chronic and acute heat stress. We apply these recommendations to data from the literature to assess chronic heat tolerance in 16 squamates, 16 turtles, five crocodilians, and the tuatara and acute heat tolerance for nine squamates and one turtle. Our results indicate that there is relatively large variation in chronic and acute heat tolerance across species, and we outline directions for future research, calling for more studies that assess embryo responses to acute thermal stress, integrate embryo responses to chronic and acute temperatures in predictive models, and identify mechanisms that determine heat tolerance. Highlights: We propose metrics for reptile embryo heat tolerance with respect to chronic and acute temperatures and describe how future studies can incorporate these metrics to advance our understanding of embryo thermal physiology. [ABSTRACT FROM AUTHOR]

Aspects of global change create stressful thermal environments that threaten biodiversity. Oviparous, non‐avian reptiles have received considerable attention because eggs are left to develop under prevailing conditions, leaving developing embryos vulnerable to increases in temperature. Though many studies assess embryo responses to long‐term (i.e., chronic), constant incubation temperatures, few assess responses to acute exposures which are more relevant for many species. We subjected brown anole (Anolis sagrei) eggs to heat shocks, thermal ramps, and extreme diurnal fluctuations to determine the lethal temperature of embryos, measure the thermal sensitivity of embryo heart rate and metabolism, and quantify the effects of sublethal but stressful temperatures on development and hatchling phenotypes and survival. Most embryos died at heat shocks of 45°C or 46°C, which is ~12°C warmer than the highest constant temperatures suitable for successful development. Heart rate and O2 consumption increased with temperature; however, as embryos approached the lethal temperature, heart rate and CO2 production continued rising while O2 consumption plateaued. These data indicate a mismatch between oxygen supply and demand at high temperatures. Exposure to extreme, diurnal fluctuations depressed embryo developmental rates and heart rates, and resulted in hatchlings with smaller body size, reduced growth rates, and lower survival in the laboratory. Thus, even brief exposure to extreme temperatures can have important effects on embryo development, and our study highlights the role of both immediate and cumulative effects of high temperatures on egg survival. Such effects must be considered to predict how populations will respond to global change. Research Highlights: At near‐lethal temperatures, CO2 production and heart rate rise, but O2 consumption plateaus, indicating a mismatch between O2 supply and demand.Brief, non‐lethal exposure to extreme temperatures has detrimental effects on embryo physiology and hatchling phenotypes and survival. [ABSTRACT FROM AUTHOR]

The use of quantitative PCR (qPCR) and other polymerase chain reaction (PCR)-based methods in the field of human in vitro fertilization blastocoel fluid analysis can potentially be utilized for assisting clinicians in embryo selection based on specific gene expression patterns. Since typical Comparative cycle threshold (Ct) analysis utilizes one threshold for runs per gene target and requires an inherent control group, this method is inadequate for analysis of small stochastic systems, such as embryonic-derived fluid. We mathematically demonstrate analytical modifications upon the Comparative Ct qPCR workflow to incorporate a variable fluorescence threshold (utilizing only the parameters defined in the Comparative Ct method), and subsequently demonstrate the typical workflow in which this modified method can successfully quantifiably analyze embryonic blastocoel fluid qPCR analysis. [ABSTRACT FROM AUTHOR]

This article presents a new paradigm that claims the existence of the psyche in the human being from the moment of conception. The author invites the reader to reconsider the outlook adopted up to now, which considers the brain the only unit of reference useful for understanding the mind and its functioning. A global vision of man cannot overlook the existence of the psyche, seen also as the soul. The human being could not live without it. In fact, the psyche appears to be present and functioning with its individual character from the moment of conception. In order to understand this new perspective, we need to look at not only what happens outside us, but also, and above all, what happens inside us, in our interiority. [ABSTRACT FROM AUTHOR]

The article presents a study highlighting bioinspired cilia arrays with programmable nonreciprocal motion and metachronal coordination. It mentions Coordinated nonreciprocal dynamics in biological cilia is essential to many living systems, where the emergent metachronal waves of cilia have been hypothesized to enhance net fluid flows at low Reynolds numbers (Re). It designs a method and developed soft miniature devices for studying ciliary biomechanics.

How gene expression can evolve depends on the mechanisms driving gene expression. Gene expression is controlled in different ways in different developmental stages; here we ask whether different developmental stages show different patterns of regulatory evolution. To explore the mode of regulatory evolution, we used the early stages of embryonic development controlled by two different genomes, that of the mother and that of the zygote. During embryogenesis in all animals, initial developmental processes are driven entirely by maternally provided gene products deposited into the oocyte. The zygotic genome is activated later, when developmental control is handed off from maternal gene products to the zygote during the maternal-to-zygotic transition. Using hybrid crosses between sister species of Drosophila (D. simulans, D. sechellia, and D. mauritiana) and transcriptomics, we find that the regulation of maternal transcript deposition and zygotic transcription evolve through different mechanisms. We find that patterns of transcript level inheritance in hybrids, relative to parental species, differ between maternal and zygotic transcripts, and maternal transcript levels are more likely to be conserved. Changes in transcript levels occur predominantly through differences in trans regulation for maternal genes, while changes in zygotic transcription occur through a combination of both cis and trans regulatory changes. Differences in the underlying regulatory landscape in the mother and the zygote are likely the primary determinants for how maternal and zygotic transcripts evolve. [ABSTRACT FROM AUTHOR]

BACKGROUND: Only a limited number of neuroimaging studies have explored the effects of ambient air pollution in adults. The prior studies have investigated only cortical volume, and they have reported mixed findings, particularly for gray matter. Furthermore, the association between nitrogen dioxide (NO2) and neuroimaging markers has been little studied in adults. OBJECTIVES: We investigated the association between long-term exposure to air pollutants (NO2, particulate matter (PM) with aerodynamic diameters of =10 µm (PM10) and =2.5 µm (PM2.5), and neuroimaging markers. RESULTS: A 10-µg/m³ increase in PM10 was associated with reduced thicknesses in the frontal [–0.02 mm (95% CI: –0.03, –0.01)] and temporal lobes [–0.06 mm (95% CI: –0.07, –0.04)]. A 10-µg/m³ increase in PM2.5 was associated with a thinner temporal cortex [–0.18 mm (95% CI: –0.27, –0.08)]. A 10-ppb increase in NO2 was associated with reduced thicknesses in the global [–0.01 mm (95% CI: –0.01, 0.00)], frontal [–0.02 mm (95% CI: –0.03, –0.01)], parietal [–0.02 mm (95% CI: –0.03, –0.01)], temporal [–0.04 mm (95% CI: –0.05, –0.03)], and insular lobes [–0.01 mm (95% CI: –0.02, 0.00)]. The air pollutants were also associated with increased thicknesses in the occipital and cingulate lobes. Subcortical structures associated with the air pollutants included the thalamus, caudate, pallidum, hippocampus, amygdala, and nucleus accumbens. DISCUSSION: The findings suggest that long-term exposure to high ambient air pollution may lead to cortical thinning and reduced subcortical volume in adults. [ABSTRACT FROM AUTHOR]