1. DNA markers to disentangle complexes of cryptic taxa in mealybugs (Hemiptera: Pseudococcidae)
- Author
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Malausa, Thibaut, Fenis, Aurore, Warot, Sylvie, Germain, J.F., Ris, Nicolas, Prado, E., Botton, M., Vanlerberghe-Masutti, Flavie, Sforza, René, Cruaud, C., Couloux, A., Kreiter, Philippe, Interactions Biotiques et Santé Végétale, Institut National de la Recherche Agronomique (INRA), Unité expérimentale de Lutte Biologique (ULB), Laboratoire National de Protection des Végétaux (LNPV), Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG), Empresa Brasileira de Pesquisa Agropecuária (Embrapa), Ministério da Agricultura, Pecuária e Abastecimento [Brasil] (MAPA), Governo do Brasil-Governo do Brasil, European Biological Control Laboratory (EBCL), United States Department of Agriculture (USDA), Genoscope - Centre national de séquençage [Evry] (GENOSCOPE), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), TIBAULT MALAUSA, INRA, A. FENNIS, INRA, S. WAROT, INRA, J.-F. GERMAIN, LNPV MONTPELLIER, N. RIS, INRA, E. PRADO, EPAMIG, MARCOS BOTTON, CNPUV, F. VANLERBERGHE-MASUTTI, INRA, R. SFORZA, EUROPEAN BIOLOGICAL CONTROL LABORATORY, C. CRUAUD, GÉNOSCOPE, A. COULOUX, GÉNOSCOPE, and P. KREITER, INRA.
- Subjects
Identificação genética ,Caracterização molecular ,[SDV]Life Sciences [q-bio] ,Entomologia ,DNA ,Genética ,Marcador Molecular ,RIBOSOMAL DNA ,COCHENILLE ,Cochonilha ,PLANOCOCCUS ,Inseto ,COCCOIDEA ,MOLECULAR CHARACTERIZATION ,PSEUDOCOCCUS ,MITOCHONDRIAL DNA - Abstract
Mealybugs (Hemiptera: Pseudococcidae) are major pests of a wide range of crops and ornamental plants worldwide. Their high degree of morphological similarity makes them difficult to identify and limits their study and management. We aimed to identify a set of markers for the genetic characterization and identification of complexes of taxa in the Pseudococcidae. We surveyed and tested the genetic markers used in previous studies and then identified new markers for particularly relevant genomic regions for which no satisfactory markers were available. We tested all markers on a subset of four taxa distributed worldwide. Five markers were retained after this first screening: two regions of the mitochondrial cytochrome oxidase I gene, 28S-D2, the entire internal transcriber space 2 locus and the rpS15-16S region of the primary mealybug endosymbiont Tremblaya princeps. We then assessed the utility of these markers for the characterization and identification of 239 samples from 43 sites in France and Brazil. The five markers studied (i) successfully distinguished all species identified by morphological examination, (ii) disentangled complexes of species by revealing intraspecific genetic variation and identified a set of closely related taxa for which taxonomic status requires clarification through further studies, and (iii) facilitated the inference of phylogenetic relationships between the characterized taxa. Made available in DSpace on 2016-10-26T21:35:54Z (GMT). No. of bitstreams: 1 BottonJAppliedEntomology2011.pdf: 351568 bytes, checksum: 04d74b3335f6d82ee407dbd92754338e (MD5) Previous issue date: 2011-01-31
- Published
- 2011