1. Oral Presentations—Abstracts
- Author
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M. Bartsch, D. K. F. Meijer, G. L. Scherphof, J. A. A. M. Kamps, S. Erdoğan, A. Y. Özer, B. Caner, H. Bilgili, L. M. Ickenstein, K. Edwards, G. Karlsson, L. D. Mayer, Crispin G. S. Eley, Ning Hu, Gerard M. Jensen, K. Kawahara, A. Sekiguchi, E. Kiyoki, K. Morimoto, O. C. Boerman, M. Miyajima, J. Kimura, G. A. Koning, H. W. M. Morselt, Josbert M. Metselaar, Marca H. M. Wauben, Otto C. Boerman, Peter L. van Lent, Gert Storm, F. Pastorino, C. Brignole, D. Marimpietri, E. H. Moase, T. M. Allen, M. Ponzoni, K. Romøren, B. J. Thu, Ø Evensen, S. Rossi, S. Ristori, G. Martini, R. M. Schiffelers, G. Molema, T. L. M. ten Hagen, A. P. C. A. Janssen, R. G. Ebben, A. J. Schraa, R. J. Kok, G. Koning, G. Storm, S. I. Simões, C. M. Marques, M. E. Cruz, G. Cevc, M. B. Martins, D. Summers, D. Ruff, R. W. Smalling, D. Cardoza, D. Dottavio, D. Lasic, J. Szebeni, L. Baranyi, S. Savay, J. Milosevits, R. Bunger, P. Laverman, J. M. Metselaar, A. Chanan-Khan, L. Liebes, F. M. Muggia, R. Cohen, Y. Barenholz, C. R. Alving, S. Hoving, A. L. B. Seynhaeve, S. T. van Tiel, A. M. M. Eggermont, K. Tokutomi, Y. Sadzuka, A. Igarashi, H. Konno, and T. Sonobe
- Subjects
Liposome ,Materials science ,Antisense oligodeoxynucleotides ,Albumin ,Cationic polymerization ,Pharmaceutical Science ,Molecular biology ,body regions ,chemistry.chemical_compound ,chemistry ,Covalent bond ,In vivo ,Mole ,Ethylene glycol - Abstract
Targeted Delivery of Antisense Oligodeoxynucleotides In Vivo by Means of Coated Cationic LipoplexesEarlier we reported on the massive uptake of liposomes surface-modified with negatively charged aconitylated albumin (Aco-HSA) by liver endothelial cells (EC) in vivo. In the present work we apply this principle for in vivo delivery of antisense oligodeoxynucleotides (ODN) to these cells by means of coated cationic lipoplexes (CCL) (). CCL were prepared by complexing ODN with the cationic lipid DOTAP and subsequent coating of the complex by neutral lipids including a lipid-anchored poly(ethylene glycol). Aco-HSA was covalently coupled.The Aco-HSA-CCLs were 160 nm in size, contained 1.03 ± 0.35 nmol ODN and 54 ± 18 µg Aco-HSA per µ mol total lipid. The Aco-HSA-CCLs were rapidly eliminated from plasma, 60% of the injected dose being recovered in the liver after 30 m. Within the liver, the EC accounted for two thirds of total liver uptake. Non-targeted CCLs were eliminated very slowly: after 30 m >90% of the pa...
- Published
- 2003
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