1,459 results on '"E Winkler"'
Search Results
2. Interprofessional evidence-based counselling programme for complementary and integrative healthcare in patients with cancer: study protocol for the controlled implementation study CCC-Integrativ
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Peter Martus, F Rapp, Hartmut Bertz, A Schmitt, M Hoffmann, B Stein, E Winkler, Stefanie Joos, Barbara Grün, M Wensing, M Reuter, K Muller, J Szecsenyi, A Kestler, Jan Valentini, Daniela Fröhlich, Regina Stolz, Cornelia Mahler, Nadja Klafke, Markus Horneber, Jona Frasch, Klaus Kramer, Katrin Tomaschko-Ubeländer, B Kröger, B Noack, B Wattenberg, E Kaschdailewitsch, H Mauch, K Gauß, K Harder, L Lohmüller, A Seckinger, D Zips, M Busacker-Scharpff, M Krug, S Eismann, B Leicht, C Nagat, C Raff, K Kraus, W Kmietschak, A Battran, A Frohn, B Held, C Perinchery, C Pfister-Jimenéz, H Dürsch, J Bossert, A Kaltenbach, U Boltenhagen, A Behzad, A Leppert, B Broge, C Witte, S Treffert, and AOK Baden-Württemberg
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Medicine - Published
- 2022
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3. You Don't Get to Say What I Believe, I Do: Provocative Encounters as Catalysts for Self-Authored Worldview Commitments during College
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Matthew J. Mayhew, Christa E. Winkler, Alyssa N. Rockenbach, and Renee L. Bowling
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This paper examines the developmental trajectories of students' self-authored worldview commitments over four years of college and the reasons they offered for making associated developmental gains. Through a longitudinal, mixed methods approach, we unearthed four distinctive developmental trajectories of a nationally-representative cohort of 9,470 students enrolled across 122 institutions. We found that provocative encounters with worldview diversity -- those that appropriately challenged students to examine their own religious commitments -- served as catalysts for growth during college. The varied contexts of those provocative encounters, both inside and outside the college classroom, are highlighted in students' own words. Implications for research are discussed.
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- 2024
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4. Facilitating comparable research in seedling functional ecology
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Daniel E. Winkler, Magda Garbowski, Kevin Kožić, Emma Ladouceur, Julie Larson, Sarah Martin, Christoph Rosche, Christiane Roscher, Mandy L. Slate, and Lotte Korell
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functional traits ,plant ontogeny ,recruitment ,seedling ,sporophyte ,Ecology ,QH540-549.5 ,Evolution ,QH359-425 - Abstract
Abstract Ecologists have worked to ascribe function to the variation found in plant populations, communities and ecosystems across environments for at least the past century. The vast body of research in functional ecology has drastically improved understanding of how individuals respond to their environment, communities are assembled and ecosystems function. However, with limited exceptions, few studies have quantified differences in plant function during the earliest stages of the plant life cycle, and fewer have tested how this early variability shapes populations, communities and ecosystems. Drawing from the literature and our collective experience, we describe the current state of knowledge in seedling functional ecology and provide examples of how this subdiscipline can enrich our fundamental understanding of plant function across levels of organisation. To inspire progressive work in this area, we also outline key considerations involved in seedling functional research (who, what, when, where and how to measure seedling traits) and identify remaining challenges and gaps in understanding around methodological approaches. Within this conceptual synthesis, we highlight three critical areas in seedling ecology for future research to target. First, given wide variation in the definition of a ‘seedling’, we provide a standard definition based on seed reserve dependence while emphasising the need to measure ontogenetic variation more clearly both within and following the seedling stage. Second, studies demonstrate that seedlings can be studied in multiple media (e.g. soil, agar, filter paper) and conditions (e.g. field, greenhouse, laboratory). We recommend that researchers select methods based on explicit goals, yet follow standard guidelines to reduce methodological noise across studies. Third, research is critically needed to assess the implications of different methodologies on trait measurement and compatibility across studies. By highlighting the importance of seedling functional ecology and suggesting pathways to address key challenges, we aim to inspire future research that generates useful and comparable data on seedling functional ecology. This work is critical to explain variation within and among populations, communities and ecosystems and integrate this most vulnerable stage of plant life into ecological frameworks.
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- 2024
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5. Reliability of continuous vital sign monitoring in post-operative patients employing consumer-grade fitness trackers: A randomised pilot trial
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Philipp Helmer, Sebastian Hottenrott, Kathrin Wienböker, Rüdiger Pryss, Vasileios Drosos, Anna Katharina Seitz, Daniel Röder, Aleksandar Jovanovic, Jürgen Brugger, Peter Kranke, Patrick Meybohm, Bernd E Winkler, and Michael Sammeth
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Computer applications to medicine. Medical informatics ,R858-859.7 - Abstract
Introduction Fitness trackers can provide continuous monitoring of vital signs and thus have the potential to become a complementary, mobile and effective tool for early detection of patient deterioration and post-operative complications. Methods To evaluate potential implementations in acute care setting, we included 36 patients after moderate to major surgery in a recent randomised pilot trial to compare the performance of vital sign monitoring by three different fitness trackers (Apple Watch 7, Garmin Fenix 6pro and Withings ScanWatch) with established standard clinical monitors in post-anaesthesia care units and monitoring wards. Results During a cumulative period of 56 days, a total of 53,197 heart rate (HR) measurements, as well as 12,219 measurements of the peripheral blood oxygen saturation (SpO 2 ) and 28,954 respiratory rate (RR) measurements were collected by fitness trackers. Under real-world conditions, HR monitoring was accurate and reliable across all benchmarked devices (r = [0.95;0.98], p
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- 2024
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6. Left ventricular global work index and prediction of cardiovascular mortality after transcatheter aortic valve implantation
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Anwer, Shehab, Nussbaum, Sinuhe, E. Winkler, Neria, C. Benz, Dominik, Zuercher, Dominik, G. Donati, Thierry, Tsiourantani, Glykeria, Wilzeck, Verena, M. Michel, Jonathan, M. Kasel, Albert, and C. Tanner, Felix
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- 2024
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7. Astrocyte 3D culture and bioprinting using peptide functionalized hyaluronan hydrogels
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Isabelle Matthiesen, Michael Jury, Fatemeh Rasti Boroojeni, Saskia L. Ludwig, Muriel Holzreuter, Sebastian Buchmann, Andrea Åman Träger, Robert Selegård, Thomas E. Winkler, Daniel Aili, and Anna Herland
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Astrocytes ,3d cell culture ,bioprinting ,hyaluronan ,cRGD ,IKVAV ,Materials of engineering and construction. Mechanics of materials ,TA401-492 ,Biotechnology ,TP248.13-248.65 - Abstract
ABSTRACTAstrocytes play an important role in the central nervous system, contributing to the development of and maintenance of synapses, recycling of neurotransmitters, and the integrity and function of the blood–brain barrier. Astrocytes are also linked to the pathophysiology of various neurodegenerative diseases. Astrocyte function and organization are tightly regulated by interactions mediated by the extracellular matrix (ECM). Engineered hydrogels can mimic key aspects of the ECM and can allow for systematic studies of ECM-related factors that govern astrocyte behaviour. In this study, we explore the interactions between neuroblastoma (SH-SY5Y) and glioblastoma (U87) cell lines and human fetal primary astrocytes (FPA) with a modular hyaluronan-based hydrogel system. Morphological analysis reveals that FPA have a higher degree of interactions with the hyaluronan-based gels compared to the cell lines. This interaction is enhanced by conjugation of cell-adhesion peptides (cRGD and IKVAV) to the hyaluronan backbone. These effects are retained and pronounced in 3D bioprinted structures. Bioprinted FPA using cRGD functionalized hyaluronan show extensive and defined protrusions and multiple connections between neighboring cells. Possibilities to tailor and optimize astrocyte-compatible ECM-mimicking hydrogels that can be processed by means of additive biofabrication can facilitate the development of advanced tissue and disease models of the central nervous system.
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- 2023
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8. Evaluating blood oxygen saturation measurements by popular fitness trackers in postoperative patients: A prospective clinical trial
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Philipp Helmer, Philipp Rodemers, Sebastian Hottenrott, Robert Leppich, Maja Helwich, Rüdiger Pryss, Peter Kranke, Patrick Meybohm, Bernd E. Winkler, and Michael Sammeth
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Health sciences ,Clinical measurement in health technology ,Bioelectronics ,Science - Abstract
Summary: Blood oxygen saturation is an important clinical parameter, especially in postoperative hospitalized patients, monitored in clinical practice by arterial blood gas (ABG) and/or pulse oximetry that both are not suitable for a long-term continuous monitoring of patients during the entire hospital stay, or beyond. Technological advances developed recently for consumer-grade fitness trackers could—at least in theory—help to fill in this gap, but benchmarks on the applicability and accuracy of these technologies in hospitalized patients are currently lacking. We therefore conducted at the postanaesthesia care unit under controlled settings a prospective clinical trial with 201 patients, comparing in total >1,000 oxygen blood saturation measurements by fitness trackers of three brands with the ABG gold standard and with pulse oximetry. Our results suggest that, despite of an overall still tolerable measuring accuracy, comparatively high dropout rates severely limit the possibilities of employing fitness trackers, particularly during the immediate postoperative period of hospitalized patients.
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- 2023
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9. Current state of the art and future directions for implantable sensors in medical technology: Clinical needs and engineering challenges
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David Yogev, Tomer Goldberg, Amir Arami, Shai Tejman-Yarden, Thomas E. Winkler, and Ben M. Maoz
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Biotechnology ,TP248.13-248.65 ,Medical technology ,R855-855.5 - Abstract
Implantable sensors have revolutionized the way we monitor biophysical and biochemical parameters by enabling real-time closed-loop intervention or therapy. These technologies align with the new era of healthcare known as healthcare 5.0, which encompasses smart disease control and detection, virtual care, intelligent health management, smart monitoring, and decision-making. This review explores the diverse biomedical applications of implantable temperature, mechanical, electrophysiological, optical, and electrochemical sensors. We delve into the engineering principles that serve as the foundation for their development. We also address the challenges faced by researchers and designers in bridging the gap between implantable sensor research and their clinical adoption by emphasizing the importance of careful consideration of clinical requirements and engineering challenges. We highlight the need for future research to explore issues such as long-term performance, biocompatibility, and power sources, as well as the potential for implantable sensors to transform healthcare across multiple disciplines. It is evident that implantable sensors have immense potential in the field of medical technology. However, the gap between research and clinical adoption remains wide, and there are still major obstacles to overcome before they can become a widely adopted part of medical practice.
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- 2023
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10. Right vs. left ventricular longitudinal strain for mortality prediction after transcatheter aortic valve implantation
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Neria E. Winkler, Shehab Anwer, Kelly A. Reeve, Jonathan M. Michel, Albert M. Kasel, and Felix C. Tanner
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aortic stenosis ,transcatheter aortic valve implantation ,global longitudinal strain ,speckle tracking echocardiography ,mortality ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
IntroductionThis study aims at exploring biventricular remodelling and its implications for outcome in a representative patient cohort with severe aortic stenosis (AS) undergoing transcatheter aortic valve implantation (TAVI).Methods and resultsPre-interventional echocardiographic examinations of 100 patients with severe AS undergoing TAVI were assessed by speckle tracking echocardiography of both ventricles. Association with mortality was determined for right ventricular global longitudinal strain (RVGLS), RV free wall strain (RVFWS) and left ventricular global longitudinal strain (LVGLS). During a median follow-up of 1,367 [959–2,123] days, 33 patients (33%) died. RVGLS was lower in non-survivors [−13.9% (−16.4 to −12.9)] than survivors [−17.1% (−20.2 to −15.2); P = 0.001]. In contrast, LVGLS as well as the conventional parameters LV ejection fraction (LVEF) and RV fractional area change (RVFAC) did not differ (P = ns). Kaplan–Meier analyses indicated a reduced survival probability when RVGLS was below the −14.6% cutpoint (P
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- 2023
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11. Primary care patients’ perspectives on the use of non-pharmacological home remedies in Geneva: a cross-sectional study
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Neria E. Winkler, Paul Sebo, Dagmar M. Haller, and Hubert Maisonneuve
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Home remedies ,Non-pharmacological treatments ,General practitioners ,Primary care ,Minor health problems ,Views ,Other systems of medicine ,RZ201-999 - Abstract
Abstract Background Home remedies are anchored in patients’ everyday life, but their use in Western cultures remains scarcely explored. Our objectives were to investigate primary care patients’ perspectives and use of non-pharmacological home remedies in Geneva (Switzerland). Methods In spring 2020, we conducted a cross-sectional survey among adult primary care patients in randomly selected general practices (N = 15). Patients were recruited in the waiting rooms and asked to complete a questionnaire about their sociodemographic characteristics, their home remedy use, and their expectations and reasons for using (or not using) home remedies. We employed descriptive statistics to summarise the data and logistic regression adjusted for clustering within practices to explore associations between home remedy use and participants’ sociodemographic characteristics. Results Three hundred fourteen of three hundred ninety patients agreed to participate in the study (participation rate 80.5%). Home remedies were used by 64.4% of patients. The main reasons given were for preventive purposes (55.3%), self-care (41.0%), as an alternative to conventional medicine (40.5%) and to avoid or delay a medical consultation (38.5%). One-third of patients considered that it was the GP’s role to spontaneously inform them about home remedies (36.4%), another third considered that it was the GP’s role to inform them, but only upon specific request (32.3%), and the last third of patients declared that it was not the GP’s role to provide information about home remedies (30.3%). Patients living in an urban zone (adjusted OR 2.1; 95%CI 1.0–4.4; p 0.05) and those with a tertiary education background (adjusted OR 1.9; 95%CI 1.0–3.6; p 0.05) believed that it was their GP’s role to inform them about home remedies. Conclusions Home remedies are used by a majority of primary care patients in Geneva. For a comprehensive and safe healthcare management in the context of patient-oriented medicine, more evidence-based research on efficacy and safety of home remedies as well as their place in primary care consultation is required.
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- 2022
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12. Accuracy and Systematic Biases of Heart Rate Measurements by Consumer-Grade Fitness Trackers in Postoperative Patients: Prospective Clinical Trial
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Philipp Helmer, Sebastian Hottenrott, Philipp Rodemers, Robert Leppich, Maja Helwich, Rüdiger Pryss, Peter Kranke, Patrick Meybohm, Bernd E Winkler, and Michael Sammeth
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Computer applications to medicine. Medical informatics ,R858-859.7 ,Public aspects of medicine ,RA1-1270 - Abstract
BackgroundOver the recent years, technological advances of wrist-worn fitness trackers heralded a new era in the continuous monitoring of vital signs. So far, these devices have primarily been used for sports. ObjectiveHowever, for using these technologies in health care, further validations of the measurement accuracy in hospitalized patients are essential but lacking to date. MethodsWe conducted a prospective validation study with 201 patients after moderate to major surgery in a controlled setting to benchmark the accuracy of heart rate measurements in 4 consumer-grade fitness trackers (Apple Watch 7, Garmin Fenix 6 Pro, Withings ScanWatch, and Fitbit Sense) against the clinical gold standard (electrocardiography). ResultsAll devices exhibited high correlation (r≥0.95; P
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- 2022
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13. Evolutionary dynamics inform management interventions of a hanging garden obligate, Carex specuicola
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Kenneth James Chapin, Matthew R. Jones, Daniel E. Winkler, Glenn Rink, and Rob Massatti
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demographic modeling ,metapopulation ,paleorefugia ,hanging garden ,phylogeography ,population genetics ,General. Including nature conservation, geographical distribution ,QH1-199.5 - Abstract
Uncovering the historical and contemporary processes shaping rare species with complex distributions is of growing importance due to threats such as habitat destruction and climate change. Species restricted to specialized, patchy habitat may persist by virtue of life history characteristics facilitating ongoing gene flow and dispersal, but they could also reflect the remnants of formerly widespread, suitable habitat that existed during past climate regimes. If formerly widespread species did not rely upon traits facilitating high dispersibility to persist, contemporary populations could be at high risk of extirpation or extinction. Fortunately, genomic investigations provide an opportunity to illuminate such alternative scenarios while simultaneously offering guidance for future management interventions. Herein, we test the role of these mechanisms in shaping patterns of genomic diversity and differentiation across a highly restricted and rare ecosystem: desert hanging gardens. We focus on Carex specuicola (Cyperaceae), a hanging garden obligate narrowly distributed in the Four Corners region of the southwestern United States that is listed as Threatened under the United States Endangered Species Act. Population structure and diversity analyses reveal that hanging garden populations are shaped by strong genetic drift, but that individuals in gardens are occasionally more closely related to individuals at other gardens than to individuals within the same garden. Similarly, gardens separated by long geographic distances may contain individuals that are more closely related compared to individuals in gardens separated by short geographic distances. Demographic modeling supports historical gene flow between some contemporary garden pairs, which is corroborated by low estimates of inbreeding coefficients and recent divergence times. As such, multiple lines of evidence support dispersal and gene flow across C. specuicola populations at both small and large spatial scales, indicating that even if C. specuicola was formerly more widespread, it may be well suited to persist in hanging gardens so long as suitable habitat remains available. Analyses like those demonstrated herein may be broadly applicable for understanding the short- and long-term evolutionary processes influencing rare species, and especially those having complex distributions across heterogeneous landscapes.
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- 2022
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14. The Evangelical Puzzle Partially Explained: Privileged Prejudice and the Development of Appreciative Attitudes Toward Evangelical Christianity
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Matthew J. Mayhew, Christa E. Winkler, B. Ashley Staples, Kevin Singer, Musbah Shaheen, and Alyssa N. Rockenbach
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appreciative attitudes ,evangelical Christian ,worldview diversity ,interfaith diversity ,higher education ,Education (General) ,L7-991 - Abstract
Background: Evangelical Christian college students simultaneously experience the privileges that accompany dominant religious identities and feel a need to conceal their identity and perspectives on college campuses. Consistently and empirically, the college campus has been studied for its potential to help students develop appreciative attitudes toward religious, secular, and spiritual worldviews. Yet, few studies have investigated evangelical Christian appreciation longitudinally over 4 years of college, and even fewer through the additional use of a mixed-methods design.Purpose: This inquiry examined if and how college students developed an appreciation of evangelical Christianity over 4 years of college.Methods: This paper used data gathered through the Interfaith Diversity Experiences and Attitudes Longitudinal Survey (IDEALS), a nationally-representative, mixed-methods study that included survey data collection from 9,470 students at 122 colleges and universities over 3 time points, and 18 qualitative case studies conducted across institutions of various sizes, locations, and affiliations.Results: Using latent growth modeling, we demonstrated that overall appreciation for evangelical Christianity developed during college and was related to institutional cultures that invited and embraced worldview diversity as well as religiously-inclusive campus climates and practices. Related qualitative insights storied change in evangelical appreciation that centered on personal relationships with evangelicals, efforts to understand evangelical viewpoints, and a recognition that Christian students often have the privilege of operating from unexamined beliefs.Conclusion and Implications: Study results provide recommendations for educational practices that support student growth from tolerance to appreciation for evangelical Christianity.
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- 2022
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15. Validation of a survey for measuring scientists' attitudes toward data reuse.
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Christa E. Winkler and Rebecca Fay Berenbon
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- 2021
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16. The use of prototypes to bridge knowledge boundaries in agile software development.
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Thomas L. Huber, Maike A. E. Winkler, Jens Dibbern, and Carol V. Brown
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- 2020
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17. Developing Innovators: A Longitudinal Analysis Over Four College Years
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Benjamin S. Selznick, Matthew J. Mayhew, Christa E. Winkler, and Eric T. McChesney
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innovation ,growth mixture modeling (GMM) ,longitudinal ,student environment ,human capital - skills ,higher education ,Education (General) ,L7-991 - Abstract
The purpose of this study is to examine the influence of collegiate environments and experiences on students’ development of innovation capacities over four years of college. Drawing on an interdisciplinary theoretical framework and reliable innovation measures, students from nine postsecondary institutions in North America were surveyed at three time points: first-year fall, first-year spring, and fourth-year spring. Data were comprehensively analyzed using a growth mixture modeling approach. Results suggest that being a transfer student and having sustained engagement with experiences that connect in-class and out-of-class learning were associated with a robust innovation growth trajectory over-and-above known covariates, including personality traits. Implications for research, theory, and practice are considered.
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- 2022
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18. Species-specific enamel differences in hardness and abrasion resistance between the permanent incisors of cattle (Bos primigenius taurus) and the ever-growing incisors of nutria (Myocastor coypus)
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Valentin L. Fischer, Daniela E. Winkler, Robert Głogowski, Thomas Attin, Jean-Michel Hatt, Marcus Clauss, and Florian Wegehaupt
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Medicine ,Science - Abstract
Hypselodont (ever-growing) teeth of lagomorphs or rodents have higher wear rates (of a magnitude of mm/week), with compensating growth rates, compared to the non-ever-growing teeth of ungulates (with a magnitude of mm/year). Whether this is due to a fundamental difference in enamel hardness has not been investigated so far. We prepared enamel samples (n = 120 per species) from incisors of cattle (Bos primigenius taurus) and nutria (Myocastor coypus, hypselodont incisors) taken at slaughterhouses, and submitted them to indentation hardness testing. Subsequently, samples were split into 4 groups per species (n = 24 per species and group) that were assessed for abrasion susceptibility by a standardized brush test with a control (no added abrasives) and three treatment groups (using fine silt at 4 ±1 μm particle size, volcanic ash at 96 ±9 μm, or fine sand at 166 ±15 μm as abrasives), in which enamel abrasion was quantified as height loss by before-and-after profilometry. The difference in enamel hardness between the species was highly significant, with nutria enamel achieving 78% of the hardness of cattle enamel. In the control and the fine sand group, no enamel height loss was evident, which was attributed to the in vitro system in the latter group, where the sand particles were brushed out of the test slurry by the brushes’ bristles. For fine silt and volcanic ash, nutria enamel significantly lost 3.65 and 3.52 times more height than cattle. These results suggest a relationship between enamel hardness and susceptibility to abrasion. However, neither the pattern within the species nor across the species indicated a monotonous relationship between hardness and height loss; rather, the difference was due to qualitative step related to species. Hence, additional factors not measured in this study must be responsible for the differences in the enamel’s susceptibility to abrasion. While the in vitro brush system cannot be used to rank abrasive test substances in terms of their abrasiveness, it can differentiate abrasion susceptibility in dental tissue of different animal species. The results caution against considering enamel wear as a similar process across mammals.
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- 2022
19. Knowledge sharing for shared success in the decade on ecosystem restoration
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Emma Ladouceur, Nancy Shackelford, Karma Bouazza, Lars Brudvig, Anna Bucharova, Timo Conradi, Todd E. Erickson, Magda Garbowski, Kelly Garvy, W. Stanley Harpole, Holly P. Jones, Tiffany Knight, Mlungele M. Nsikani, Gustavo Paterno, Katharine Suding, Vicky M. Temperton, Péter Török, Daniel E. Winkler, and Jonathan M. Chase
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data synthesis ,dissemination ,ecological restoration ,evidence‐based knowledge ,networks ,open data ,Environmental sciences ,GE1-350 ,Ecology ,QH540-549.5 - Abstract
Abstract The Decade on Ecosystem Restoration aims to provide the means and incentives for upscaling restoration efforts worldwide. Although ecosystem restoration is a broad, interdisciplinary concept, effective ecological restoration requires sound ecological knowledge to successfully restore biodiversity and ecosystem services in degraded landscapes. We emphasize the critical role of knowledge and data sharing to inform synthesis for the most robust restoration science possible. Such synthesis is critical for helping restoration ecologists better understand how context affects restoration outcomes, and to increase predictive capacity of restoration actions. This predictive capacity can help to provide better information for evidence‐based decision‐making, and scale‐up approaches to meet ambitious targets for restoration. We advocate for a concerted effort to collate species‐level, fine‐scale, ecological community data from restoration studies across a wide range of environmental and ecological gradients. Well‐articulated associated metadata relevant to experience and social or landscape contexts can further be used to explain outcomes. These data could be carefully curated and made openly available to the restoration community to help to maximize evidence‐based knowledge sharing, enable flexible re‐use of existing data and support predictive capacity in ecological community responses to restoration actions. We detail how integrated data, analysis and knowledge sharing via synthesis can support shared success in restoration ecology by identifying successful and unsuccessful outcomes across diverse systems and scales. We also discuss potential interdisciplinary solutions and approaches to overcome challenges associated with bringing together subfields of restoration practice. Sharing this knowledge and data openly can directly inform actions and help to improve outcomes for the Decade on Ecosystem Restoration.
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- 2022
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20. The centralised sale of football media rights in Europe
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Slobodan Sudaric, C.-Philipp Heller, and Anne E. Winkler
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History ,Polymers and Plastics ,Economics, Econometrics and Finance (miscellaneous) ,Football ,Industrial and Manufacturing Engineering ,Competition (economics) ,Relevant market ,Market economy ,Common value auction ,Business ,Club ,Business and International Management ,Law ,Market definition - Abstract
We analyse the competitive effects of the centralised sale of football media rights in Europe, focusing on the “Big Five” countries (England, France, Germany, Italy, Spain). Contrary to the recent findings of European competition authorities, we consider that there are arguments in favour of the relevant market for domestic media rights being club- or even match-specific. This raises the question of what competition is restricted by the centralised sale if the rights on offer do not compete. If the media rights of different clubs are indeed complementary and because of the potential efficiency gains from bundling, we conclude that the centralised sale of media rights is unlikely to be anticompetitive and may have procompetitive effects. We further show that under a club or match-specific market definition, a no-single-buyer rule likely reduces the benefits from the centralised sale and may be to the detriment of consumers.
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- 2023
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21. You Don’t Get to Say What I Believe, I Do: Provocative Encounters as Catalysts for Self-Authored Worldview Commitments During College
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Matthew J. Mayhew, Christa E. Winkler, Alyssa N. Rockenbach, and Renee L. Bowling
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Education - Published
- 2023
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22. Man: The Bridge Between Two Worlds
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Franz E. Winkler
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- 2019
23. Endophyte-Promoted Phosphorus Solubilization in Populus
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Tamas Varga, Kim K. Hixson, Amir H. Ahkami, Andrew W. Sher, Morgan E. Barnes, Rosalie K. Chu, Anil K. Battu, Carrie D. Nicora, Tanya E. Winkler, Loren R. Reno, Sirine C. Fakra, Olga Antipova, Dilworth Y. Parkinson, Jackson R. Hall, and Sharon L. Doty
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Populus ,poplar ,endophytes ,phosphorus ,solubilization ,synchrotron x-ray fluorescence ,Plant culture ,SB1-1110 - Abstract
Phosphorus is one of the essential nutrients for plant growth, but it may be relatively unavailable to plants because of its chemistry. In soil, the majority of phosphorus is present in the form of a phosphate, usually as metal complexes making it bound to minerals or organic matter. Therefore, inorganic phosphate solubilization is an important process of plant growth promotion by plant associated bacteria and fungi. Non-nodulating plant species have been shown to thrive in low-nutrient environments, in some instances by relying on plant associated microorganisms called endophytes. These microorganisms live within the plant and help supply nutrients for the plant. Despite their potential enormous environmental importance, there are a limited number of studies looking at the direct molecular impact of phosphate solubilizing endophytic bacteria on the host plant. In this work, we studied the impact of two endophyte strains of wild poplar (Populus trichocarpa) that solubilize phosphate. Using a combination of x-ray imaging, spectroscopy methods, and proteomics, we report direct evidence of endophyte-promoted phosphorus uptake in poplar. We found that the solubilized phosphate may react and become insoluble once inside plant tissue, suggesting that endophytes may aid in the re-release of phosphate. Using synchrotron x-ray fluorescence spectromicroscopy, we visualized the nutrient phosphorus inside poplar roots inoculated by the selected endophytes and found the phosphorus in both forms of organic and inorganic phosphates inside the root. Tomography-based root imaging revealed a markedly different root biomass and root architecture for poplar samples inoculated with the phosphate solubilizing bacteria strains. Proteomics characterization on poplar roots coupled with protein network analysis revealed novel proteins and metabolic pathways with possible involvement in endophyte enriched phosphorus uptake. These findings suggest an important role of endophytes for phosphorus acquisition and provide a deeper understanding of the critical symbiotic associations between poplar and the endophytic bacteria.
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- 2020
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24. Warming of alpine tundra enhances belowground production and shifts community towards resource acquisition traits
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Yan Yang, Julia A. Klein, Daniel E. Winkler, Ahui Peng, Brynne E. Lazarus, Matthew J. Germino, Katharine N. Suding, Jane G. Smith, and Lara M. Kueppers
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alpine tundra ,belowground plant production ,functional traits ,Rocky Mountains ,soil moisture ,warming ,Ecology ,QH540-549.5 - Abstract
Abstract Climate warming is expected to stimulate plant growth in high‐elevation and high‐latitude ecosystems, significantly increasing aboveground net primary production (ANPP). However, the effects of simultaneous changes in temperature, snowmelt timing, and summer water availability on total net primary production (NPP)—and elucidation of both above‐ and belowground responses—remain an important area in need of further study. In particular, measures of belowground net primary productivity (BNPP) are required to understand whether ANPP changes reflect changes in allocation or are indicative of a whole plant NPP response. Further, plant functional traits provide a key way to scale from the individual plant to the community level and provide insight into drivers of NPP responses to environmental change. We used infrared heaters to warm an alpine plant community at Niwot Ridge, Colorado, and applied supplemental water to compensate for soil water loss induced by warming. We measured ANPP, BNPP, and leaf and root functional traits across treatments after 5 yr of continuous warming. Community‐level ANPP and total NPP (ANPP + BNPP) did not respond to heating or watering, but BNPP increased in response to heating. Heating decreased community‐level leaf dry matter content and increased total root length, indicating a shift in strategy from resource conservation to acquisition in response to warming. Water use efficiency (WUE) decreased with heating, suggesting alleviation of moisture constraints that may have enabled the plant community to increase productivity. Heating may have decreased WUE by melting snow earlier and creating more days early in the growing season with adequate soil moisture, but stimulated dry mass investment in roots as soils dried down later in the growing season. Overall, this study highlights how ANPP and BNPP responses to climate change can diverge, and encourages a closer examination of belowground processes, especially in alpine systems, where the majority of NPP occurs belowground.
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- 2020
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25. Experimental Warming Changes Phenology and Shortens Growing Season of the Dominant Invasive Plant Bromus tectorum (Cheatgrass)
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Armin Howell, Daniel E. Winkler, Michala L. Phillips, Brandon McNellis, and Sasha C. Reed
- Subjects
Bromus tectorum ,climate change ,dryland ,invasive plants ,phenology ,phenophase ,Plant culture ,SB1-1110 - Abstract
Bromus tectorum (cheatgrass) has successfully invaded and established throughout the western United States. Bromus tectorum grows early in the season and this early growth allows B. tectorum to outcompete native species, which has led to dramatic shifts in ecosystem function and plant community composition after B. tectorum invades. If the phenology of native species is unable to track changing climate as effectively as B. tectorum’s phenology then climate change may facilitate further invasion. To better understand how B. tectorum phenology will respond to future climate, we tracked the timing of B. tectorum germination, flowering, and senescence over a decade in three in situ climate manipulation experiments with treatments that increased temperatures (2°C and 4°C above ambient), altered precipitation regimes, or applied a combination of each. Linear mixed-effects models were used to analyze treatment effects on the timing of germination, flowering, senescence, and on the length of the vegetative growing season (time from germination to flowering) in each experiment. Altered precipitation treatments were only applied in early years of the study and neither precipitation treatments nor the treatments’ legacies significantly affected B. tectorum phenology. The timing of germination did not significantly vary between any warming treatments and their respective ambient plots. However, plots that were warmed had advances in the timing of B. tectorum flowering and senescence, as well as shorter vegetative growing seasons. The phenological advances caused by warming increased with increasing degrees of experimental warming. The greatest differences between warmed and ambient plots were seen in the length of the vegetative growing season, which was shortened by approximately 12 and 7 days in the +4°C and +2°C warming levels, respectively. The effects of experimental warming were small compared to the effects of interannual climate variation, suggesting that interactive controls and the timing of multiple climatic factors are important in determining B. tectorum phenology. Taken together, these results help elucidate how B. tectorum phenology may respond to future climate, increasing our predictive capacity for estimating when to time B. tectorum control efforts and how to more effectively manage this exotic annual grass.
- Published
- 2020
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- View/download PDF
26. Supporting Joint Idea Generation with Software Prototypes in Offshore-Outsourced Software Development Projects.
- Author
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Maike A. E. Winkler, Thomas L. Huber, and Jens Dibbern
- Published
- 2016
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27. Roles of <scp>RodZ</scp> and class A <scp>PBP1b</scp> in the assembly and regulation of the peripheral peptidoglycan elongasome in ovoid‐shaped cells of Streptococcus pneumoniae <scp>D39</scp>
- Author
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Melissa M. Lamanna, Irfan Manzoor, Merrin Joseph, Ziyun A. Ye, Mattia Benedet, Alessia Zanardi, Zhongqing Ren, Xindan Wang, Orietta Massidda, Ho‐Ching T. Tsui, and Malcolm E. Winkler
- Subjects
Molecular Biology ,Microbiology - Published
- 2022
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- View/download PDF
28. Spatially explicit management of genetic diversity using ancestry probability surfaces
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Rob Massatti and Daniel E. Winkler
- Subjects
Ecological Modeling ,Ecology, Evolution, Behavior and Systematics - Published
- 2022
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- View/download PDF
29. Advanced skills in quantitative assessment
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Christa E. Winkler
- Subjects
General Medicine - Published
- 2022
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30. Local temporal trajectories explain population‐level responses to climate change in saguaro (Carnegiea gigantea)
- Author
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Susana Rodríguez‐Buriticá, Daniel E. Winkler, Robert H. Webb, and D. Lawrence Venable
- Subjects
Carnegiea gigantea ,climate change ,demography ,desert plants ,drought ,extreme temperatures ,Ecology ,QH540-549.5 - Abstract
Abstract Population demography is typically assumed to be strongly influenced by climatic factors, particularly with succulent plants and cacti. The saguaro cactus (Carnegiea gigantea) is a long‐lived columnar cactus of the Sonoran Desert that experiences episodic recruitment and mortality. Previous studies have attributed long‐term changes in saguaro populations to climatic factors, including increased germination and establishment during wet periods and mortality and reduced establishment during droughts and extreme freezes. We used a 48‐yr data set of marked individuals at the Desert Laboratory in Tucson, Arizona, to test the hypothesis that local, temporal population trajectories are mediated by topographic heterogeneity that interacts with fluctuating climatic conditions. We tested the influence of local slope and aspect vs. climatic variability on a population of saguaro using >5800 marked individuals that have been measured since 1964. We examined the relationship between demography and climatic variables (drought, precipitation, and extreme temperatures) and found significant differences in growth and survival among aspects and among census periods. Saguaro population growth was higher during wet and cool periods (e.g., 1964–1970), and changes in age structures suggest that topographic differences interact with climatic fluctuations to produce unexpected demographic patterns including large recruitment events during periods of relatively unfavorable climate conditions. Our results highlight the importance of long‐term data to detect demographic responses to climate that could not be predicted from short‐term studies of plant physiology and population demography.
- Published
- 2019
- Full Text
- View/download PDF
31. Supplementary Figures 1 - 7 from A Surprising Cross-Species Conservation in the Genomic Landscape of Mouse and Human Oral Cancer Identifies a Transcriptional Signature Predicting Metastatic Disease
- Author
-
Ravindra Uppaluri, Elaine R. Mardis, James S. Lewis, Jay F. Piccirillo, Gavin P. Dunn, Nancy P. Judd, Dorina Kallogjeri, Charles G. Rickert, Jonathan H. Law, Varun Chalivendra, Krishna-Latha Kanchi, Ashley E. Winkler, and Michael D. Onken
- Abstract
PDF file - 486KB, Figure S1 (A) Average statistics for depth of coverage at 20X, 30X and 40X of all MOC lines. Robust reads were obtained in all samples with a range of 97-98 percent for 20X, 93-95 percent for 30X and 88-92 percent for 40X. (B) Putative SNVs, nsSNVs (Table S1 and S2) and Indels (Table S4) of all MOC lines. Figure S2 Oncoprints from cBio of AKAP9, MED12L, THSD7A, MUC5B, MYH6, LAMA1, LRP2, and 3 RAS (Table S6) genes representing other candidate tumor promoters as compared to the 3 RAS genes. Note that for AKAPs and MED components, we found 9 AKAP family member mutations in 20.4 percent of tumors, with AKAP9 changes in 7 percent (Fig. 1D). Six components of the mediator complex were mutated in 14.7% of cases, with MED12L changes in 5% (Fig. 1E). Figure S3 Number of nsSNV's per node negative (N0) and node positive (N+) tumor in (A) all TCGA OSCC (note that patient TCGA-D6-6516 (N0) with 1463 mutations is not included in this graph) and (B) TCGA OSCC patients who had smoking history reported. There was no significant difference in the average number of mutations between N0 (3272 nsSNVs) and N+ (3097 nsSNVs) patients regardless of smoking status (Tables S8, S9). This analysis showed 17 genes commonly mutated in mouse indolent and human N0 tumors and 55 common genes mutated in mouse aggressive and human N+ tumors (Supplementary Table S10). However, none of these common genes were mutated at high frequency in the human N0 or N+ datasets (Supplementary Table S11 and 12). Finally, comparing N0 and N+ tumors from human TCGA data also showed that specific mutations occur infrequently in both the metastatic and non-metastatic tumors (data not shown). Figure S4 SAM plotsheet of MOC line microarray data with estimated miss rates for delta=4.68 Figure S5 (A) GSEA data of OCAMP-A on UW/FHCRC data with (B) first condensation of enriched genes. (C) GSEA data of OCAMP-A on MDA data with (D) first condensation of enriched genes. (E) GSEA data of OCAMP-A on TCGA data with (F) first condensation of enriched genes. Figure S6 (A) Disease specific survival (DSS) after weighted voting classification of OCAMP-B signature on the MD Anderson dataset shows worse outcome for those with aggressive classification (p=0.028). Figure S7 (A) Disease specific survival (DSS) and (B) overall survival (OS) after weighted voting classification of OCAMP-B signature on the UW/FHCRC dataset shows worse outcome for those with aggressive classification (p
- Published
- 2023
- Full Text
- View/download PDF
32. Data from A Surprising Cross-Species Conservation in the Genomic Landscape of Mouse and Human Oral Cancer Identifies a Transcriptional Signature Predicting Metastatic Disease
- Author
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Ravindra Uppaluri, Elaine R. Mardis, James S. Lewis, Jay F. Piccirillo, Gavin P. Dunn, Nancy P. Judd, Dorina Kallogjeri, Charles G. Rickert, Jonathan H. Law, Varun Chalivendra, Krishna-Latha Kanchi, Ashley E. Winkler, and Michael D. Onken
- Abstract
Purpose: Improved understanding of the molecular basis underlying oral squamous cell carcinoma (OSCC) aggressive growth has significant clinical implications. Herein, cross-species genomic comparison of carcinogen-induced murine and human OSCCs with indolent or metastatic growth yielded results with surprising translational relevance.Experimental Design: Murine OSCC cell lines were subjected to next-generation sequencing (NGS) to define their mutational landscape, to define novel candidate cancer genes, and to assess for parallels with known drivers in human OSCC. Expression arrays identified a mouse metastasis signature, and we assessed its representation in four independent human datasets comprising 324 patients using weighted voting and gene set enrichment analysis. Kaplan–Meier analysis and multivariate Cox proportional hazards modeling were used to stratify outcomes. A quantitative real-time PCR assay based on the mouse signature coupled to a machine-learning algorithm was developed and used to stratify an independent set of 31 patients with respect to metastatic lymphadenopathy.Results: NGS revealed conservation of human driver pathway mutations in mouse OSCC, including in Trp53, mitogen-activated protein kinase, phosphoinositide 3-kinase, NOTCH, JAK/STAT, and Fat1-4. Moreover, comparative analysis between The Cancer Genome Atlas and mouse samples defined AKAP9, MED12L, and MYH6 as novel putative cancer genes. Expression analysis identified a transcriptional signature predicting aggressiveness and clinical outcomes, which were validated in four independent human OSCC datasets. Finally, we harnessed the translational potential of this signature by creating a clinically feasible assay that stratified patients with OSCC with a 93.5% accuracy.Conclusions: These data demonstrate surprising cross-species genomic conservation that has translational relevance for human oral squamous cell cancer. Clin Cancer Res; 20(11); 2873–84. ©2014 AACR.
- Published
- 2023
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- View/download PDF
33. Supplementary Figure Legends from A Surprising Cross-Species Conservation in the Genomic Landscape of Mouse and Human Oral Cancer Identifies a Transcriptional Signature Predicting Metastatic Disease
- Author
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Ravindra Uppaluri, Elaine R. Mardis, James S. Lewis, Jay F. Piccirillo, Gavin P. Dunn, Nancy P. Judd, Dorina Kallogjeri, Charles G. Rickert, Jonathan H. Law, Varun Chalivendra, Krishna-Latha Kanchi, Ashley E. Winkler, and Michael D. Onken
- Abstract
PDF file - 81KB
- Published
- 2023
- Full Text
- View/download PDF
34. Supplementary Tables 1 - 20 from A Surprising Cross-Species Conservation in the Genomic Landscape of Mouse and Human Oral Cancer Identifies a Transcriptional Signature Predicting Metastatic Disease
- Author
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Ravindra Uppaluri, Elaine R. Mardis, James S. Lewis, Jay F. Piccirillo, Gavin P. Dunn, Nancy P. Judd, Dorina Kallogjeri, Charles G. Rickert, Jonathan H. Law, Varun Chalivendra, Krishna-Latha Kanchi, Ashley E. Winkler, and Michael D. Onken
- Abstract
XLS file - 6534KB, S1 SNVs indolent lines. S2 SNVs aggressive lines. S3 Distribution of nucleotide changes. S4 MOC line mutations have similarity to 32 most common HNSCC TCGA mutations. S5 MOC line conservation with human OSCC drivers and novel mutations. S6 Indels all MOC lines. S7 Comparison of predicted changes between aggressive and indolent lines. S8 MOC line common mutations. S9 Unique aggressive MOC line mutations. S10 Comparison of MOC line mutations with TCGA N0 and N+ mutations. S11 TCGA mutation burden per tumor in N0 and N+ specimens. S12 MOC line and TCGA common mutations. S13 Rate of alterations of aggressive MOC/TCGA N+ mutations from Table S10. S14 Rate of alterations of indolent MOC/TCGA N0 mutations from Table S10. S15 SAM analysis of aggressive versus indolent expression data. S16 Compiled rank metric scores of OCAMP-A genes across three human datasets. S17 Cross tabulation and multivariable analysis of OCAMP-B on MD dataset. S18 Weighted voting classification with OCAMP-B on independent UPENN dataset. S19 SVM output data for training and test sets. S20 OCAMP-A comparison to three published signatures.
- Published
- 2023
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- View/download PDF
35. Supplementary Figures 1-3 from CXCR3 Enhances a T-Cell–Dependent Epidermal Proliferative Response and Promotes Skin Tumorigenesis
- Author
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Ravindra Uppaluri, Robert D. Schreiber, James S. Lewis, Nancy P. Judd, Meredith E. Pittman, Joshua J. Brotman, and Ashley E. Winkler
- Abstract
PDF file - 402K
- Published
- 2023
- Full Text
- View/download PDF
36. Supplementary Methods, Figures 1-10 from ERK1/2 Regulation of CD44 Modulates Oral Cancer Aggressiveness
- Author
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Ravindra Uppaluri, John B. Sunwoo, Jack D. Bui, Gavin P. Dunn, James S. Lewis, Jonathan H. Law, Joshua J. Brotman, Oihana Murillo-Sauca, Ashley E. Winkler, and Nancy P. Judd
- Abstract
PDF file - 2.3MB
- Published
- 2023
- Full Text
- View/download PDF
37. Chromosomal Duplications of MurZ (MurA2) or MurA (MurA1), Amino Acid Substitutions in MurZ (MurA2), and Absence of KhpAB Obviate the Requirement for Protein Phosphorylation inStreptococcus pneumoniaeD39
- Author
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Ho-Ching Tiffany Tsui, Merrin Joseph, Jiaqi J. Zheng, Amilcar J. Perez, Irfan Manzoor, Britta E. Rued, John D. Richardson, Pavel Branny, Linda Doubravová, Orietta Massidda, and Malcolm E. Winkler
- Subjects
Article - Abstract
GpsB links peptidoglycan synthases to other proteins that determine the shape of the respiratory pathogenStreptococcus pneumoniae(pneumococcus;Spn) and other low-GC Gram-positive bacteria. GpsB is also required for phosphorylation of proteins by the essential StkP(Spn) Ser/Thr protein kinase. Here we report three classes of frequently arising chromosomal duplications (≈21-176 genes) containingmurZ(MurZ-family homolog of MurA) ormurAthat suppress ΔgpsBor ΔstkP. These duplications arose from three different repeated sequences and demonstrate the facility of pneumococcus to modulate gene dosage of numerous genes. Overproduction of MurZ or MurA alone or overexpression of MurZ caused by ΔkhpABmutations suppressed ΔgpsBor ΔstkPphenotypes to varying extents. ΔgpsBand ΔstkPwere also suppressed by MurZ amino-acid changes distant from the active site, including one in commonly studied laboratory strains, and by truncation or deletion of the homolog of IreB(ReoM). Unlike in other Gram-positive bacteria, MurZ is predominant to MurA in pneumococcal cells. However, ΔgpsBand ΔstkPwere not suppressed by ΔclpCP, which did not alter MurZ or MurA amounts. These results support a model in which regulation of MurZ and MurA activity, likely by IreB(Spn), is the only essential requirement for protein phosphorylation in exponentially growing D39 pneumococcal cells.
- Published
- 2023
- Full Text
- View/download PDF
38. Restoration research actions to address rapid change in drylands: insights from the Colorado Plateau
- Author
-
Kristina E. Young, Brooke B. Osborne, Michala Phillips, and Daniel E. Winkler
- Subjects
Ecology ,Ecology, Evolution, Behavior and Systematics ,Nature and Landscape Conservation - Published
- 2023
- Full Text
- View/download PDF
39. Record Linkage
- Author
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Peter Christen and William E. Winkler
- Published
- 2023
- Full Text
- View/download PDF
40. Surface texture analysis in Toothfrax and MountainsMap® SSFA module: Different software packages, different results?
- Author
-
Ivan Calandra, Konstantin Bob, Gildas Merceron, François Blateyron, Andreas Hildebrandt, Ellen Schulz-Kornas, Antoine Souron, and Daniela E. Winkler
- Published
- 2022
- Full Text
- View/download PDF
41. Low-Cost PVD Shadow Masks with Submillimeter Resolution from Laser-Cut Paper
- Author
-
Farzad Elhami Nik, Isabelle Matthiesen, Anna Herland, and Thomas E. Winkler
- Subjects
shadow mask ,stencil lithography ,CO2 laser ,paper ,metal deposition ,Mechanical engineering and machinery ,TJ1-1570 - Abstract
We characterize an affordable method of producing stencils for submillimeter physical vapor deposition (PVD) by using paper and a benchtop laser cutter. Patterning electrodes or similar features on top of organic or biological substrates is generally not possible using standard photolithography. Shadow masks, traditionally made of silicon-based membranes, circumvent the need for aggressive solvents but suffer from high costs. Here, we evaluate shadow masks fabricated by CO2 laser processing from quantitative filter papers. Such papers are stiff and dimensionally stable, resilient in handling, and cut without melting or redeposition. Using two exemplary interdigitated electrode designs, we quantify the line resolution achievable with both high-quality and standard lenses, as well as the positional accuracy across multiple length scales. Additionally, we assess the gap between such laser-cut paper masks and a substrate, and quantify feature reproduction onto polycarbonate membranes. We find that ~100 µm line widths are achievable independent of lens type and that average positional accuracy is better than ±100 µm at 4”-wafer scale. Although this falls well short of the micron-size features achievable with typical shadow masks, resolution in the tenths to tens of millimeters is entirely sufficient for applications from contact pads to electrochemical cells, allowing new functionalities on fragile materials.
- Published
- 2020
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- View/download PDF
42. Modeling Meaningful Use as Utility in Emergency Medical Services.
- Author
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Steven R. Haynes, Thomas E. Winkler, and Frank E. Ritter
- Published
- 2013
- Full Text
- View/download PDF
43. Snowmelt Timing Regulates Community Composition, Phenology, and Physiological Performance of Alpine Plants
- Author
-
Daniel E. Winkler, Ramona J. Butz, Matthew J. Germino, Keith Reinhardt, and Lara M. Kueppers
- Subjects
distribution ,elevation ,flowering ,Niwot Ridge ,photosynthesis ,snowmelt gradient ,Plant culture ,SB1-1110 - Abstract
The spatial patterning of alpine plant communities is strongly influenced by the variation in physical factors such as temperature and moisture, which are strongly affected by snow depth and snowmelt patterns. Earlier snowmelt timing and greater soil-moisture limitations may favor wide-ranging species adapted to a broader set of ecohydrological conditions than alpine-restricted species. We asked how plant community composition, phenology, plant water relations, and photosynthetic gas exchange of alpine-restricted and wide-ranging species differ in their responses to a ca. 40-day snowmelt gradient in the Colorado Rocky Mountains (Lewisia pygmaea, Sibbaldia procumbens, and Hymenoxys grandiflora were alpine-restricted and Artemisia scopulorum, Carex rupestris, and Geum rossii were wide-ranging species). As hypothesized, species richness and foliar cover increased with earlier snowmelt, due to a greater abundance of wide-ranging species present in earlier melting plots. Flowering initiation occurred earlier with earlier snowmelt for 12 out of 19 species analyzed, while flowering duration was shortened with later snowmelt for six species (all but one were wide-ranging species). We observed >50% declines in net photosynthesis from July to September as soil moisture and plant water potentials declined. Early-season stomatal conductance was higher in wide-ranging species, indicating a more competitive strategy for water acquisition when soil moisture is high. Even so, there were no associated differences in photosynthesis or transpiration, suggesting no strong differences between these groups in physiology. Our findings reveal that plant species with different ranges (alpine-restricted vs. wide-ranging) could have differential phenological and physiological responses to snowmelt timing and associated soil moisture dry-down, and that alpine-restricted species’ performance is more sensitive to snowmelt. As a result, alpine-restricted species may serve as better indicator species than their wide-ranging heterospecifics. Overall, alpine community composition and peak % cover are strongly structured by spatio-temporal patterns in snowmelt timing. Thus, near-term, community-wide changes (or variation) in phenology and physiology in response to shifts in snowmelt timing or rates of soil dry down are likely to be contingent on the legacy of past climate on community structure.
- Published
- 2018
- Full Text
- View/download PDF
44. A common garden super‐experiment: An impossible dream to inspire possible synthesis
- Author
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Kailen A. Mooney, Travis E. Huxman, and Daniel E. Winkler
- Subjects
Plant ecology ,Ecology ,media_common.quotation_subject ,Environmental ethics ,Plant Science ,Dream ,Biology ,Ecosystem ecology ,Ecology, Evolution, Behavior and Systematics ,media_common - Published
- 2021
- Full Text
- View/download PDF
45. Demographic modeling informs functional connectivity and management interventions in Graham’s beardtongue
- Author
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Matthew R. Jones, Daniel E. Winkler, and Robert Massatti
- Subjects
education.field_of_study ,Demographic history ,Range (biology) ,Ecology (disciplines) ,Rare species ,Population ,Biodiversity ,Biology ,Gene flow ,Evolutionary biology ,Genetic variation ,Genetics ,education ,Ecology, Evolution, Behavior and Systematics - Abstract
Functional connectivity (i.e., the movement of individuals across a landscape) is essential for the maintenance of genetic variation and persistence of rare species. However, illuminating the processes influencing functional connectivity and ultimately translating this knowledge into management practice remains a fundamental challenge. Here, we combine various population structure analyses with pairwise, population-specific demographic modeling to investigate historical functional connectivity in Graham’s beardtongue (Penstemon grahamii), a rare plant narrowly distributed across a dryland region of the western US. While principal component and population structure analyses indicated an isolation-by-distance pattern of differentiation across the species’ range, spatial inferences of effective migration exposed an abrupt shift in population ancestry near the range center. To understand these seemingly conflicting patterns, we tested various models of historical gene flow and found evidence for recent admixture (~ 3400 generations ago) between populations near the range center. This historical perspective reconciles population structure patterns and suggests management efforts should focus on maintaining connectivity between these previously isolated lineages to promote the ongoing transfer of genetic variation. Beyond providing species-specific knowledge to inform management options, our study highlights how understanding demographic history may be critical to guide conservation efforts when interpreting population genetic patterns and inferring functional connectivity.
- Published
- 2021
- Full Text
- View/download PDF
46. Sorption of Neuropsychopharmaca in Microfluidic Materials for In Vitro Studies
- Author
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Anna Herland and Thomas E. Winkler
- Subjects
Materials science ,Other Engineering and Technologies not elsewhere specified ,Microfluidics ,microfluidics ,Peristaltic pump ,02 engineering and technology ,01 natural sciences ,complex mixtures ,materials ,chemistry.chemical_compound ,Adsorption ,Lab-On-A-Chip Devices ,Övrig annan teknik ,General Materials Science ,neuropsychopharmaca ,Dimethylpolysiloxanes ,Polycarbonate ,organs-on-chips ,Fluorescent Dyes ,Polydimethylsiloxane ,010401 analytical chemistry ,non-specific binding ,Sorption ,Microfluidic Analytical Techniques ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Device material ,chemistry ,Chemical engineering ,visual_art ,visual_art.visual_art_medium ,Absorption (chemistry) ,0210 nano-technology ,Hydrophobic and Hydrophilic Interactions ,Research Article ,Central Nervous System Agents - Abstract
Sorption (i.e., adsorption and absorption) of small-molecule compounds to polydimethylsiloxane (PDMS) is a widely acknowledged phenomenon. However, studies to date have largely been conducted under atypical conditions for microfluidic applications (lack of perfusion, lack of biological fluids, etc.), especially considering biological studies such as organs-on-chips where small-molecule sorption poses the largest concern. Here, we present an in-depth study of small-molecule sorption under relevant conditions for microphysiological systems, focusing on a standard geometry for biological barrier studies that find application in pharmacokinetics. We specifically assess the sorption of a broad compound panel including 15 neuropsychopharmaca at in vivo concentration levels. We consider devices constructed from PDMS as well as two material alternatives (off-stoichiometry thiol–ene–epoxy, or tape/polycarbonate laminates). Moreover, we study the much neglected impact of peristaltic pump tubing, an essential component of the recirculating systems required to achieve in vivo-like perfusion shear stresses. We find that the choice of the device material does not have a significant impact on the sorption behavior in our barrier-on-chip-type system. Our PDMS observations in particular suggest that excessive compound sorption observed in prior studies is not sufficiently described by compound hydrophobicity or other suggested predictors. Critically, we show that sorption by peristaltic tubing, including the commonly utilized PharMed BPT, dominates over device sorption even on an area-normalized basis, let alone at the typically much larger tubing surface areas. Our findings highlight the importance of validating compound dosages in organ-on-chip studies, as well as the need for considering tubing materials with equal or higher care than device materials. QC 20211020
- Published
- 2021
47. Perspectives on challenges and opportunities at the restoration‐policy interface in the U.S.A
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Ella M. Samuel, Rachel M. Mitchell, and Daniel E. Winkler
- Subjects
Ecology ,Ecology, Evolution, Behavior and Systematics ,Nature and Landscape Conservation - Published
- 2022
- Full Text
- View/download PDF
48. First application of dental microwear texture analysis to infer theropod feeding ecology
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Daniela E. Winkler, Tai Kubo, Mugino O. Kubo, Thomas M. Kaiser, and Thomas Tütken
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Paleontology ,Ecology, Evolution, Behavior and Systematics - Published
- 2022
- Full Text
- View/download PDF
49. Use of running plates by floor housed rats: A pilot study
- Author
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Thomas Tütken, Louise F. Martin, Jessica Frei, Daniela E. Winkler, Marcus Clauss, University of Zurich, and Martin, Louise F
- Subjects
Animal Experimentation ,10253 Department of Small Animals ,3400 General Veterinary ,media_common.quotation_subject ,Pilot Projects ,Motor Activity ,Animal Welfare ,Agricultural economics ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Animals ,0501 psychology and cognitive sciences ,050102 behavioral science & comparative psychology ,media_common ,630 Agriculture ,General Veterinary ,05 social sciences ,Animal husbandry ,Rats ,570 Life sciences ,biology ,Animal Science and Zoology ,Business ,1103 Animal Science and Zoology ,Sleep ,human activities ,Welfare ,030217 neurology & neurosurgery - Abstract
The outfit of husbandry facilities of, and the enrichment provided for, experimental rodents plays an important role in the animals’ welfare, and hence also for the societal acceptance of animal experiments. Whether rats and mice benefit from being provided with running wheels or plates is discussed controversially. Here we present observations from a feeding experiment, where rats were provided a running plate. As a pilot study, six identical cages, with three animals per cage, were filmed for six days, and the resulting footage was screened for the number of bouts and the time the animals spent on the plates. The main activities observed on the plate in descending order were sitting (18.5 ± 13.8 bouts or 8.0 ± 13.7 min/animal per day), standing (10.2 ± 11.6 bouts, 3.8 ± 4.2 min), running (8.2 ± 13.3 bouts, 10.1 ± 21.4 min), grooming (2.0 ± 2.8 bouts, 6.7 ± 25.7 min), sleeping (1.0 ± 2.6 bouts, 24.0 ± 61.8 min) and playing (0.5 ± 0.9 bouts, 0.1 ± 0.5 min). Most of these activities (91% of all bouts, 90% of total time) occurred at night, similar to previous studies on running wheel usage. The running plate seems well-accepted as cage enrichment, even though in further studies, the motivating triggers and the effects of long-term use could be evaluated more in-depth.
- Published
- 2021
- Full Text
- View/download PDF
50. Drivers of seedling establishment success in dryland restoration efforts
- Author
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Qinfeng Guo, Kevin Z. Mganga, Suanne Jane Milton, Réka Kiss, Philip J. Burton, Peter J. Golos, Monica L. Pokorny, Péter Török, Magda Garbowski, Carla M. Burton, Jeremy J. James, Matt A. Bahm, Scott D. Wilson, Carina Becker, Luis Merino-Martín, Anita Kirmer, Barry Heydenrych, Peter A. Harrison, Matthew J. Rinella, Megan Wong, Eric W. Seabloom, Darin J. Law, Jessica Drake, Nelmarie Saayman, Sandra Dullau, Nichole N. Barger, Seth M. Munson, Pablo Luis Peri, Zhiwei Xu, Merilynn C. Schantz, Owen W. Baughman, Balázs Deák, Juan Lorite, Katharine L. Stuble, Eman Calleja, Orsolya Valkó, C. Ellery Mayence, Kirk W. Davies, Kari E. Veblen, Joshua Eldridge, Daniel E. Winkler, Penelope A. Grey, Akasha M. Faist, R. Emiliano Quiroga, Ali Abdullahi, Arlee M. Montalvo, Enrique G. de la Riva, Elizabeth A. Leger, Martin F. Breed, Shauna M. Uselman, Manuel Esteban Lucas-Borja, Charlie D. Clements, Julie E. Larson, Todd E. Erickson, Lauren N. Svejcar, Patricia M. Holmes, Tamás Miglécz, Elizabeth A. Ballenger, Chad S. Boyd, Thomas A. Monaco, Erin K. Espeland, Lauren M. Porensky, Hannah L. Farrell, Peter J. Carrick, Mark W. Paschke, Jose A. Navarro-Cano, Nancy Shackelford, Tina Parkhurst, Jayne Jonas-Bratten, Andrea T. Kramer, Claire E. Wainwright, Stephen E. Fick, Michael F. Curran, Alex Caruana, Gustavo Brant Paterno, Katharine L. Suding, Shackelford, Nancy, Paterno, Gustavo B, Breed, Martin F, Harrison, Peter A, Guo, Qinfeng, Kirmer, Anita, Munson, Seth M, Török, Péter, Becker, Carina, Burton, Philip J, Caruana, Alex, Deák, Balázs, Dullau, Sandra, Golos, Peter J, Holmes, Patricia M, Jonas-Bratten, Jayne, Lorite, Juan, Merino-Martín, Luis, Milton, Suanne Jane, Seabloom, Eric W, Valkó, Orsolya, Veblen, Kari, Xu, Zhiwei, and Suding, Katharine L
- Subjects
Ecology ,Agroforestry ,Seedling ,Climate Change ,media_common.quotation_subject ,food and beverages ,Climate change ,Introduced species ,Plant ,Vegetation ,Plants ,Livelihood ,Geography ,Desertification ,Seedlings ,Seeds ,Plant seed ,Humans ,Forb ,Seeding ,Restoration ecology ,Ecosystem ,Ecology, Evolution, Behavior and Systematics ,Human ,media_common - Abstract
20 Pág. Departamento de Medio Ambiente y Agronomía, Restoration of degraded drylands is urgently needed to mitigate climate change, reverse desertification and secure livelihoods for the two billion people who live in these areas. Bold global targets have been set for dryland restoration to restore millions of hectares of degraded land. These targets have been questioned as overly ambitious, but without a global evaluation of successes and failures it is impossible to gauge feasibility. Here we examine restoration seeding outcomes across 174 sites on six continents, encompassing 594,065 observations of 671 plant species. Our findings suggest reasons for optimism. Seeding had a positive impact on species presence: in almost a third of all treatments, 100% of species seeded were growing at first monitoring. However, dryland restoration is risky: 17% of projects failed, with no establishment of any seeded species, and consistent declines were found in seeded species as projects matured. Across projects, higher seeding rates and larger seed sizes resulted in a greater probability of recruitment, with further influences on species success including site aridity, taxonomic identity and species life form. Our findings suggest that investigations examining these predictive factors will yield more effective and informed restoration decision-making., We would like to thank the supporters of the Global Arid Zone Project. The intellectual and energetic input of the network participants made this work possible. We also acknowledge the many employers and funding agencies that supported projects and the authors’ time in preparing this work and contributing data to the GAZP database. Please note that any use of trade, firm or product names is for descriptive purposes only and does not imply endorsement by the US Government
- Published
- 2021
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