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This work presents results of long-term phase-contrast microscopy research of proliferative potential of tumor cell lines utilizing live-cell imaging technology Cell-IQ (Chip-Man Technologies Ltd, Finland). It was found that the machine vision technology allowed to obtain sufficient body of evidence about high-quality and quantitative changes of proliferative activity of the tumor cells cultivated in static conditions. The present study demonstrates that modeling of time interval of maximum proliferative activity of tumors cells increases information efficacy and reliability of the analysis of dividing cell patterns using Cell-IQ technology. The models of exponential growth of various tumor cell lines, describing their quantitative and dynamic changes of expansion potential have been received. Modeling of maximum tumor cells proliferative activity can be applied for development of test-system of individual cell sensitivity to anticancer drugs in vitro.

The immunotropic effects of the probiotic complex Bactistatin (the complex contains metabolites of Bacillus subtilis) used simultaneously with oral doxycycline during different periods of time (1, 5, 10 and 20 days) were estimated. The dynamics of the changes in the nonspecific immunity factors under the effect of the above mentioned products or their combination (bactistatin + doxycycline) during the treatment of different duration was demonstrated. It was found that the oral administration of the metabolic probiotic bactistatin to experimental animals during 1-10 days increased the activity of the nonspecific resistance factors. The immunotropic effects of the product were evident at the level of the cells of the blood phagocytic system and the processes related to synthesis of such enzymes as lysozyme and myeloperoxidase and their release into the blood stream. Comparatively rapid increase of the functional activity of the factors was registered even after a single administration of the product. When the combination of the products (bactistatin + doxycycline) was administered to the animals during a fixed period of time (10 days) significant activation of the nonspecific immunity factors was observed. In perspective the findings will allow to optimize the basic regimens of antibacterial therapy and urgent (postcontact) prevention of infectious diseases.

Characteristics of Bacillus subtilis metabolites contained in supernatants of its broth cultures are described. Metabolites contained bacterium-produced biologically active components ensuring cells growth and propagation. These components had bacteriostatic and bactericidal effect on gram-positive and gram-negative pathogenic and conditionally pathogenic microflora of gastrointestinal tract of human and animals. Enzymes produced by the bacterium (amylase, protease, cellulose-decomposing enzyme, lipase, pectinase) increased antagonistic properties of preparation and promote its probiotic effect.

Approaches to increasing the efficacy of the regimens for emergency prevention and treatment of infectious diseases with combined use of antibiotics, immunomodulators and probiotic metabolites are examined. On the basis of multi-factorial statistical analysis optimization of the use of such agents is discussed.