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Your search keyword '"E H, van den Bogaard"' showing total 3 results
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3 results on '"E H, van den Bogaard"'

1. The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culture

Author

S. Di Blasio, G. F. van Wigcheren, A. Becker, A. van Duffelen, M. Gorris, K. Verrijp, I. Stefanini, G. J. Bakker, M. Bloemendal, A. Halilovic, A. Vasaturo, G. Bakdash, S. V. Hato, J. H. W. de Wilt, J. Schalkwijk, I. J. M. de Vries, J. C. Textor, E. H. van den Bogaard, M. Tazzari, and C. G. Figdor

Subjects
Science
Abstract

Conventional co-culture systems often lack physiological complexity of the tumor microenvironment. Here, the authors report an organotypic skin melanoma culture and use this model to investigate the tumor induced suppression on dendritic cells.

Published
2020
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2. Expression profile of cornified envelope structural proteins and keratinocyte differentiation-regulating proteins during skin barrier repair

Author

H D, de Koning, E H, van den Bogaard, J G M, Bergboer, M, Kamsteeg, I M J J, van Vlijmen-Willems, K, Hitomi, J, Henry, M, Simon, N, Takashita, A, Ishida-Yamamoto, J, Schalkwijk, and P L J M, Zeeuwen

Subjects
Keratinocytes, Wound Healing, Transglutaminases, Biopsy, Down-Regulation, Cell Differentiation, Filaggrin Proteins, Dermatitis, Atopic, Cornified Envelope Proline-Rich Proteins, Case-Control Studies, Chronic Disease, Humans, Psoriasis, RNA, Messenger, Epidermis
Abstract

Recent studies have emphasized the importance of heritable and acquired skin barrier abnormalities in common inflammatory diseases such as psoriasis and atopic dermatitis (AD). To date, no comprehensive studies on the effect of experimental barrier disruption on cornified envelope protein expression have been performed.To analyse the effect of experimental skin barrier disruption on the expression of cornified envelope structural proteins and keratinocyte differentiation-regulating proteins.We examined mRNA (day 1, 3 and 7) and protein (day 1, 2, 4 and 9) expression levels of structural proteins and regulatory molecules after sodium dodecyl sulphate (SDS) application on normal skin, and tape stripping of uninvolved epidermis of patients with psoriasis and AD and healthy controls.Upon tape stripping, several structural molecules were significantly downregulated (at the mRNA level as well as the protein level), including LCE5A, LCE2B, FLG, FLG2 and LOR, whereas others were upregulated: IVL, SPRR1, SPRR2, HRNR and most notably LCE3A. The epidermal crosslinking enzymes TGM1, TGM3 and TGM5 were all upregulated, whereas proteases involved in the desquamation process (CTSV, KLK5 and KLK7) were downregulated or unaffected. Most results were similar in SDS-instigated irritant contact dermatitis. There was no significant difference in response between normal epidermis and nonlesional skin of patients with psoriasis and AD.Skin barrier disruption induces a temporary barrier repair response composed of increased expression of several cornification-related proteins, and decreased expression of some structural and desquamation-related proteins.

Published
2012

3. Targeting the Complexity of In Vitro Skin Models: A Review of Cutting-Edge Developments.

Author

Quílez C, Bebiano LB, Jones E, Maver U, Meesters L, Parzymies P, Petiot E, Rikken G, Risueño I, Zaidi H, Zidarič T, Bekeschus S, H van den Bogaard E, Caley M, Colley H, López NG, Letsiou S, Marquette C, Maver T, Pereira RF, Tobin DJ, and Velasco D

Subjects
Humans, Models, Biological, Skin Diseases drug therapy, Skin Diseases therapy, Animals, Skin Aging physiology, Skin Aging drug effects, Tissue Engineering methods, Skin Physiological Phenomena, In Vitro Techniques, Skin cytology, Skin pathology
Abstract

Skin in vitro models offer much promise for research, testing drugs, cosmetics, and medical devices, reducing animal testing and extensive clinical trials. There are several in vitro approaches to mimicking human skin behavior, ranging from simple cell monolayer to complex organotypic and bioengineered 3-dimensional models. Some have been approved for preclinical studies in cosmetics, pharmaceuticals, and chemicals. However, development of physiologically reliable in vitro human skin models remains in its infancy. This review reports on advances in in vitro complex skin models to study skin homeostasis, aging, and skin disease., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)

Published
2024
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