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Objective: A comparison of the prevalence of intestinal parasites (IP) in preschoolers and orphans in the Warmia-Masuria province (Poland)., Material and Methods: Between 2003-2006 fecal samples and perianal swabs from 1052 preschoolers and 859 orphans were tested on the basis of direct saline and iodine mount, decantation test and the adhesive cellophane tape method., Results: 10.8% of preschoolers and 46,3% of orphans were infected with IP. Among the six detected IP species the E. vermicularis was the most common. Infections of E. vermicularis were diagnosed in 9.5% of preschoolers and 36,7% of orphans. There were statistically significant differences in the distribution of IP between males and females in preschools. IP infections were the most frequent among 7-year-old children, 19.1% in preschools and 65.7% in orphanages, respectively. In preschools, the prevalence of IP was higher among preschoolers from the rural area (17.3%) than from the urban area (10.3%)., Conclusion: Orphans and children from rural areas, especially at the age of 7, should be covered by systematic screening with parasitic tests throughout all of Poland. In this group of children, enterobiasis is the main health problem. The promotion of the awareness of IP infections and their prevention among parents and educational staff is required.

Between 2009-2010, a group of 259 patients suspected of contracting Lyme disease were examined in the Provincial Sanitary-Epidemiological Station in Olsztyn for the presence of IgM and IgG against specific Borrelia burgdorferi sensu lato (s.l.) genospecies antigens by immunoblot. A total of 27.4% and 29.0% of the blood serum samples showed positive and uncertain results for IgM and IgG antibodies. The majority of positive and uncertain results were found in patients aged 30-40 years (30%) for IgM, and people aged 50 and over (35.8%) for IgG. Significantly more positive results for IgG were found in males (40.2%) then females (19.7%). In both groups, similar proportions of positive results for IgM anti-Borrelia were recorded (26.1% of females and 29% of males. In 71.5% of patients, IgM against flagellin protein (p41) of B. burgdorferi sensu stricto (s.s.) was found. For IgG, the most frequently detected antibodies were found against the p41 protein of B. burgdorferi s.s. (64.8%) and the recombinant variable surface antigens (VlsE) (49%). Among all the analysed antigens those of B. burgdorferi s.s. were the most frequent cause of immunological reaction, followed by antigens of B. afzelii and B. garinii. Reaction to antigens of B. spielmanii was rarely detected.

The aim of the study was to analyze of tick-borne encephalitis (TBE) in the Warmia-Masuria province on the basis of serological testing of 878 patients with symptoms of neurological infections, diagnosed in 2006-2010 in the Voivodeship Sanitary-Epidemiological Station in Olsztyn. Specific IgM and IgG antibodies against the TBE virus were detected in serum and cerebrospinal fluid samples by ELISA method. Percentage of persons with serologically confirmed TBE was 15.5%. This confirms contact with TBE virus and its role as the suspected cause of neurological infections in patients in the Warmia-Masuria province. Men and people over 46 years of age were more commonly seropositive. Among examined patients the percentage of seropositive men was 18.1% and the proportion of seropositive women was - 12.9%. Most positive results were found in patients between 46 and 50 years of age (20.7%) and in those aged 56-60 years (19.5%). Seasonal patterns were detected, with highest proportion of seropositive results in July, August and October.

This article treats of infections submitted as food poisoning examples caused by Shigella flexneri 3a which is an etiological factor of diseases characterized by different clinical picture. The aim of the research was to test our laboratory specific procedure in diagnostics of Shigella infections. The stool was inoculated on three following solid culture media: salmonella - Shigella Agar (SS), Mac Conkey Agar (MC), Hektoen Enteric Agar (HE) and one liquid medium selenite - phosphate medium (SF). Classical biochemical and serological tests were carried out to ascertain the presence of the colonies suspected of being formed by pathogenic bacteria. The isolation of Shigella flexneri 3a strains were from specimens collected from peoples connected with three different outbreaks of food poisonings confirms the usefulness of the above mentioned procedure in laboratory of epidemiological and clinical investigations of outbreaks. (LBEK).