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Creativity in biology education is a crucial aspect that enhances students’ understanding of the subject. It consists of several indicators (fluency, flexibility, originality, and elaboration), which can also be used as measuring tools for creativity. This study aimed to determine the influence of the Problem-Oriented Project-Based Learning (POPBL) model in fostering creativity among high school students. The experiment was conducted in April until June 2023. The participants of this study consisted of 10th-grade students from State High School 1 Grogol. The cluster random sampling technique selected X6 as the control and X7 as the experimental classes. This was a quasi-experiment with a Pretest-Posttest Control Group Design, in which two groups were chosen randomly where the classes had previously been tested for equality first. The analysis employed was an analysis of the covariance test. The statistical analysis revealed that students’ creativity in the experimental group was significantly higher than in the control group. Based on this study, we conclude that the POPBL model significantly improved student creativity. We suggest that with higher creativity, students will be able to solve the problems they face in life better.

Superbugs revert to bacterial strains, which exhibit resistance to antibiotics. These bacteria could cause some problems in disease treatment and the environment. This research aimed of this study was to identify the presence of superbugs in the river stream that flows through Malang State University campus area. Amoxicillin, tetracycline, and chloramphenicol were selected to test the possible bacterial resistance. Water samples taken from a decided spot were spread over Luria Bertani’s agar on both antibiotic supplemented and antibiotics-free media with no delay. Molecular identification was carried out using 16S rRNA gene DNA barcoding approach, completed with morphological and Gram staining analyses. A total of 16 isolates of gram-negative colonies were found in the form of bacilli, diplobacilli, cocci, and diplococci. The genetic identification of eight resistant colonies led us to suggest that the isolates may belong to Aeromonas, Shigella, and Bacillus. Further studies are still required to get a clearer view of the correct taxonomical position of those resistant isolates.

Science has implemented the cell cultures as the primary for research such as a model system, toxicity tests, cancer, viruses, cell-based industries, genetic counseling, genetic engineering, gene therapy, drug screening and the latest is invitro meat. The purpose of this study was to determine the resistance of chicken embryo eksplan cells in invitro media. The research design was in the form of laboratory experiments. Explants taken 4 day old chick embryos, which were cultured in MEM media + serum. The results showed fibroblast cell cultures which survive 2 days. The most influential factor is the environment that is less supportive of the growth of explant chicken embryo cell

COVID-19 is a pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus. The first case was found in the city of Wuhan, Hubei province, China. The first case in Indonesia was reported in March 2020 and currently there are 0.5 million cases with a death rate of 3.1%. This rapid increase in cases is thought to due to presence of the mutant strain S-D614G, which causes a faster rate of infection and spread. The purpose of this study was to determine the presence of S-D614G mutations in Indonesian samples in order to find the origin of COVID-19 which was spread in Indonesia based on the Spike gene sequences and the RdRp genes from 25 countries, and one control sequence China/Wuhan-Hu-1 obtained from the NCBI and GISAID databases. Mutation analysis was carried out through multiple alignments using BioEdit software. Phylogenetic tree reconstruction using MEGA6 software with the Neighbor Joining method. This study found mutation of S-D614G in one Indonesian sample, namely the Indonesian/SBY9 sample along with 23 samples from Europe, America, and Africa. The phylogenetic tree reconstruction confirmed these findings; the mutated samples were closely related to samples from these continents, while the non-mutated Indonesian samples were closely related to sample from East Asia. These findings indicate that the origin of the SARS-CoV-2 virus in Indonesia possibly came from the East Asia cluster and the European-American cluster.

The Incidence of Indonesia breast cancer case in2018 was reportedat 20.7% or160,653 in number. The factors that caused breast cancer is TP53gene mutationand Single Nucleotide Polymorphism (SNP). This study aimedto determine the mutation and Single Nucleotide Polymorphism (SNP) of TP53genein breast cancer. Samples ofthis study were 9 people chosen based on a purposive tech-nique. The methods include total DNA isolation, DNA quantification, PCR, and sequencing. The results of the sequencingwere then analyzed using alignment and blast. The SNP is browsedby the SNP finder on NCBI both followed by protein modeling. The results of this study indicate the existence of mutation in the exon and intron regions. Substitution of Guanine (G) to base Adenine (A)is occur in codon 496 which is a coding region. SNP was also obtained in exon of sample 12. The conclusion of this study is breast cancer can be caused by mutations that occur in the exonand altered the structur of protein structure, so then affect the binding affinity between p53 and its target domain.Tp53 gene mutation is not the only cause of breast cancer staging development, there are other genes that also con-tribute to the development of cancer stage.In addition, Single Nucleotide Poly-morphism in the TP53gene can be used as a predisposition marker of breast cancer that has potential to be inherited.

SARS-CoV-2 is a virus that has caused COVID-19 pandemic. This virus is a new variant of the SARS-CoV virus and also closely related to MERS-CoV, which caused similar acute respiratory infections. All these viruses recognize target cells by binding to the Receptor Binding Domain (RBD) on Spike protein with receptors. This study aimed to determine the SARS-CoV-2, MERS-CoV, and SARS-CoV genome structure, Spike protein sequence differences, and variations of RBDs Receptor Binding Motif (RBM). This research was using data mining approach. Genome sequences were downloaded from NCBI, except for Indonesian samples were downloaded from GISAID. Genomic structures, Spike sequence, and RBD structure were analyzed using Bioedit, followed by protein modelling using SwissModel and PyMol applications. The result showed that the SARS-CoV-2, MERS-CoV, and SARS-CoV genome shared the same genes yet in different numbers and length. SARS-CoV-2 Spike protein sequence was quite similar to SARS-CoV Spike protein, but very different to the Spike protein of MERS-CoV. There were variations of RBDs RBM structure due to the mutations occurred among these viruses. It is suggested that these differences may increase the affinity between SARS-CoV-2 Spike protein to its hACE2 receptor which caused SARS-CoV-2 becomes more infective and spread wider than SARS-CoV and MERS-CoV, in turn. This result expected to be basic information for the development of SARS-CoV-2 introduction inhibition agent and spreading prevention.

Abstract Background The identification of banana cultivars genome is needed to provide a valid identity from the accession of bananas which are used as basic data in the management of in situ and ex situ banana conservation as well as for further breeding of banana cultivars. Results The size of PCR-amplified matK ranged from 844 to 860 bp and showed a high variability. The haplotype diversity was 0.9048 with nine haplotypes. Haplotype distribution map revealed the lineage patterns of banana cultivars from Java. Reconstruction of genetic relationships using the maximum likelihood, maximum parsimony and Bayesian inference algorithms produces tree topologies and classifications that are grouped according to their genomic groups, into three main clades, i.e., AA/AAA, AAB and ABB. Based on the previously derived age constraints and fossil data, we estimate (Musaceae) that genetic divergence times of all samples occurred during the Eocene (95% HPD: 51.9 Mya), Musa acuminata group (AA, AAA, AAB) with Musa balbisiana group (BB and ABB) occurred during the Oligocene (95% HPD: 26 Mya), and the separation on each banana cultivars occurred during the Middle Miocene to Pliocene (95% HPD: 16.5–2.5 Mya). Conclusions From this study, we conclude that all studied cultivars are closely related according to its genomic groups with high variation. Genetic variation among those cultivars creates nine haplotypes. The development of variety which leads to the formation of different banana cultivars had suggested to be occurred long ago along with human migration and domestication.

Virus-Bacteria Diagnostic Test (VBD-Test) is three-tier diagnostic instrument for identification virus and bacteria topics. The purpose of this study was to determine the empirical validity of the VBD-Test and identify misconceptions of virus and bacteria topics in biology teachers in Malang using VBD-Test. The development research model used was the Treagust development model, namely (1) Defining content, (2) Obtaining information, and (3) Developing a diagnostic test. The empirical validity on the VBD-Test was carried out with the Pearson correlation test using Excel, while the reliability on the VBD-Test was carried out with a spearman brown split-half method using Anatest 4.0.2. There are six valid questions and eight invalid questions on the virus topic, while in bacteria topics, there are nine valid questions and six invalid questions. The results of the identification of the teacher’s concept of the virus topic were 33.33% understood the idea, 13.66% false positive, 35.53% false negative, 17.01% did not understand the concept, and 4.39% guessed. While the results of the identification of the teacher’s concept of bacteria topic were 30.13% understood the concept, 9.07% false positive, 227.63% false negative, 23.05% did not understand the concept, and 10.09% guessed. Based on these results, if identification misconception is not made to the teacher, then the misconception will not be known by the teacher on any material, and the learning material submitted by the teacher will remain a misconception so that students will have misconception.

Rice nutrition including vitamin and amylose contents become important aspect for many people around the world. Rice with high amylose content (low glycemic index) is good for those with Diabetes mellitus. Tocotrienol, one precursor of Vitamin-E biosynthesis is catalyzed by enzymes encoded HGGT, while amylose biosynthesis is catalyzed by enzymes encoded GBSSI. The objective of this study was to find rice varieties with high tocotrienol and/or amylose content based on the expression of HGGT and GBSSI among eight Banyuwangi local rice varieties. Relative expression of HGGT and GBSSI was measured by qRT-PCR and analyzed using 2ΔCt method. Statistical analysis resulted in the significantly different of HGGT and GBSSI relative expression among samples. Relative expression of HGGT from the highest to the lowest were demonstrated by Hitam Melik, Hitam Pekat, Blambangan A3, Merah Bali, Blambangan A2, Berlian, Janur Kuning, and SOJ A3, respectively; while relative expression of GBSSI from the highest to the lowest were demonstrated by Hitam Melik, Hitam Pekat, SOJ A3, Janur Kuning, Berlian, Merah Bali, Blambangan A3, and Blambangan A2, respectively. Based on this research we conclude that Hitam Melik potentially produces higher tocotrienol and lower glycemic index than other studied varieties.

Indonesia possesses vast coastal agricultural land areas with high salinity. This high salinity is a threat to rice productivity which may decrease to 79.8%. Banten province of Indonesia houses many local rice varieties which have salinity tolerance potency based on its agronomical characters. The adaptability of rice plant in high salinity soil can be affected by OsDREB2A gene, which encodes transcription factors from several salt-tolerant signaling pathway genes. The aims of this study were to find out the possible polymorphism of OsDREB2A gene from Banten local rice varieties. The polymorphism of OsDREB2A analyzed by Single Nucleotide Polymorphism (SNP) and possible molecular structure. OsDREB2A gene was amplified using DNA from Tambleng, Bulu Putih, and Pare Caok varieties as templates through PCR method with a pair of specific primers. DNA fragments obtained were analyzed by means of series of analysis software. From those analysis we obtained 840 bp, 836 bp, and 850 bp gene fragments from Tambleng, Bulu Putih, and Pare Caok varieties, respectively. There is polymorphism of OsDREB2A due to some mutation yet does not massively alter its protein structure. From this study, we suggest that Tambleng, Bulu Putih, and Pare Caok varieties potential for salinity stress resistance genetically.

Four bacterial isolates from Ranu Pani and Ranu Grati in east java had been revealed to be potentials to produce IAA (PIS isolate), phosphate solubilizer (GPS isolate), cellulose hydrolysis (PSS isolate) and, amylum hydrolysis (PAS), two dominant bacterial isolates from Rani Pani (PØD isolate) and Ranu Grati (GØD isolate) which were co-cultured with microalgae promoted microalgae growth, yet its taxonomical position has not been clearly known. The aim of this study was to identify those bacterial isolates using 16S rRNA barcode. This research conducted by gDNA isolation, the 16S rRNA sequence was amplified using 27F and 1492R primers. Reconstructed phylogenetic trees and genetic distance analysis showed that the isolate PIS and PSS identified as Bacillus cereus Group closely related to Bacillus paramycoides. PAS isolate identified as Bacillus subtilis Group closely related to Bacillus amyloliquefaciens, GPS isolate identified as novel species in genus Enterobacter, and two dominant isolates (PØD and GØD) identified as Enterobacter cloacae complex closely related to Enterobacter cloacae. The genomic approach and additional phenotypes-examination are required to clarify its taxonomical position.

Abstract: Learning is expected to provide learning experiences to students in order to understand learning material. The contextuality of learning resources and learning media must be improved. Biotechnology Handout "Utilization of Jackfruit Dami as the Baseline for Making Nata" is used to guide students to use learning media. This study aims to determine the effect of handouts on the ability to think creatively. This research is a quasi-experimental design with the pretest-posttest control group design method, using one control class and one treatment class as the research sample. Data analysis uses gain score and independent t-test. Based on the t-test, the Sig value is obtained. 2-tailed (0,000)

Abstract: The purpose of this research was to find out the teaching materials that needed to be developed in Cell Biology courses at Malang City Universities. The study was conducted on students who have taken Cell Biology courses in four college at the Malang city. The type of research conducted is quantitative descriptive research. The results showed that students were interested in Cell Biology courses and in the Cell Biology course there were several topics which were considered difficult including cell division, cell division control, and cancer, while for the results of the study related to the availability of teaching materials used, data were obtained that in three universities, namely UNISMA, UMM, and IKIP Budi Utomo, the percentage was more than 50% while for UIN the percentage was 48%. Students consider teaching that has not been able to construct understanding of concepts in studying Cell Biology courses so it is necessary to develop research-based module teaching materials. Abstrak: Tujuan penelitian ini adalah untuk mengetahui bahan ajar yang perlu dikembangkan pada matakuliah Biologi Sel di Perguruan Tinggi Kota Malang. Penelitian dilakukan terhadap mahasiswa yang telah menempuh matakuliah Biologi Sel di empat Perguruan Tinggi kota Malang. Jenis penelitian yang dilakukan adalah penelitian deksriptif kuantitatif. Hasil penelitian menunjukkan bahwa mahasiswa tertarik pada matakuliah Biologi Sel dan pada matakuliah Biologi Sel terdapat beberapa topik yang dianggap sulit antara lain pembelahan sel, pengendalian pembelahan sel, dan kanker. Hasil penelitian terkait ketersediaan bahan ajar yang digunakan diperoleh data bahwa pada tiga perguruan tinggi yaitu UNISMA, UMM, dan IKIP Budi Utomo diperoleh persentase lebih dari 50% sedangkan untuk UIN didapatkan persentase sebesar 48%. Mahasiswa menganggap ajar yang digunakan belum dapat mengonstruk pemahaman konsep dalam mempelajari matakuliah Biologi Sel sehingga perlu adanya pengembangan bahan ajar modul berbasis penelitian.

Indonesia is tropical country with high risk of tropical disease. Students need to develop life skills to cope that challenges. Therefore research to determine the effect of tropical diseases in problem-based learning on students' life skills need to be established. This experimental research was used nonrandomized control group pretest-posttest design. The subjects were 66 high school students in Malang, 35 students as an experimental group and 31 students as a control group. Students' life skill was measured using test and data were analyzed using Anacova. The results showed that tropical disease problem-based learning significantly enhance students' life skills (p < 0.05). Indonesia merupakan negara tropis yang berisiko tinggi terhadap masalah penyakit tropis. Siswa perlu mengembangkan kecakapan hidup untuk menghadapi tantangan masalah tersebut. Sehingga perlu dilakukan penelitian yang bertujuan untuk mengetahui pengaruh pemanfaatan penyakit tropis dalam pembelajaran berbasis masalah terhadap kecakapan hidup siswa. Penelitian ini menggunakan nonrandomized control group pretest-posttest design. Subjek penelitian adalah 66 siswa SMA di Kabupaten Malang, 35 siswa sebagai kelompok eksperimen dan 31 siswa sebagai kelompok kontrol. Kecakapan hidup siswa diukur menggunakan soal pretes-postes dan hasilnya dianalisis menggunakan anakova. Hasil penelitian menunjukkan bahwa pembelajaran berbasis masalah penyakit tropis secara signifikan meningkatkan kecakapan hidup siswa (p < 0.05).

The purpose of this study was to determine the effect of learning model Problem-Based Field Investigation toward questioning skill of biology students of the State University of Malang (UM). This research quasi experimental elaborated One Group Time-Series Design. The study was conducted on 26 students who take a Limnology course. Data were collected from the observation and questionnaire before and after the application of the model. Based on the results of Analysis of Variance (ANOVA) with significance level of 0.05 (P value

This purpose of this research was to determine the influence of scientific inquiry based learning on science process skills. This experimental research was used nonrandomized control group pretest-posttest design. Population was all students of class XI SMAN 7 Malang. Samples in this study consists of two classes, XI MIPA 1 as experimental group and XI MIPA 2 as the control group. The independent variable was scientific inquiry based learning. The dependent variable was science processes skills. The research result show that scientific inquiry based learning significantly increase the student’s science process skills (p

Booklet is one of the learning media prepared for training or counseling for the community. The fruit craftsman community needs a booklet on "Nata de jambu, rich fiber fermented food " to help study the nata from Guava Darsono production. The booklets were prepared based on the experimental research result on "The Effect of Guava Darsono and Varieties and Sugar sorts on Nata Quality Based on the Thickness, Weight and Nata Fiber. The purpose of this study is to produce a booklet about nata from Guava Darsono production. This booklet development model uses the Hannafin & Peck development model. The booklet validator consists of: media expert validator, material expert validator, and 15 person fruit processing craftsman for legibility test respondent. Data collection technique by questionnaire. The research results is a booklet with the title "Nata de jambu, Rich Fiber Fermented Food " has been made. The validator's assessment stated that this booklet is worthy to be used as a learning media for fruit craftsmen. Booklet adalah salah satu media belajar yang disusun untuk keperluan pelatihan atau penyuluhan bagi masyarakat. Masyarakat pengrajin olahan buah membutuhkan booklet tentang “Nata de jambu, makanan fermentasi kaya serat” untuk membantu mempelajari pembuatan nata dari jambu Darsono. Booklet disusun berdasarkan hasil penelitian eksperimen tentang “Pengaruh Varietas Jambu Darsono dan Macam Gula terhadap Kualitas Nata Berdasarkan Tebal, Berat dan Kadar Serat Nata. Tujuan penelitian ini yaitu untuk menghasilkan booklet tentang pembuatan nata dari jambu Darsono. Model pengembangan booklet ini menggunakan model pengembangan Hannafin & Peck. Validator booklet terdiri atas validator ahli media, ahli materi, dan responden yaitu pengrajin olahan buah berjumlah 15 orang untuk uji keterbacaan. Teknik Pengumpulan data dilakukan melalui penyebaran angket. Hasil penelitian ini adalah Booklet dengan judul “Nata de jambu, Makanan Fermentasi Kaya Serat” telah tersusun. Hasil penilaian para validator menyatakan booklet ini layak digunakan sebagai media pembelajaran bagi para pengrajin olahan buah.

This research aims to develop the virtual laboratory learning media in genetic engineering course. This type of this reseach is development. Data is collected with observation, interview, and distribution of questionnaire. Validation result shows that the virtual laboratory learning media is valid to use for genetic engineering course. It shows the result of subject expert (56,3%), media expert (57,1%), and lecturer (65,6%). Students give good response in the virtual laboratory learning media for genetic engineering course. Penelitian ini bertujuan untuk mengembangkan media pembelajaran virtual laboratory untuk matakuliah rekayasa genetika. Jenis penelitian ini adalah pengembangan. Data dikumpulkan dengan melakukan observasi, wawancara, dan penyebaran angket. Hasil validasi ahli, media pembelajaran virtual laboratory layak digunakan untuk matakuliah rekayasa genetika. Hal ini dapat dilihat dari hasil validasi ahli materi sebesar 56,3%, ahli media sebesar 57,1%, dan praktisi lapangan sebesar 65,6%. Mahasiswa memberikan respon baik untuk media pembelajaran virtual laboratory untuk matakuliah rekayasa genetika.

Chili pepper is widely used and cultivated by Indonesian people. There are three species of chili pepper, i.e.: Capsicum annuum L., Capsicum frutescens L., and Capsicum violaceum HBK. Capsicum frutescens L. has a higher economic value due to its pungency and carotenoid content. C. frutescens has several cultivars, one of those is Capsicum frutescens cv. Cakra Hijau. This cultivar is resistant against pest and disease and has very high pungency. This special character of chili pepper is born by its secondary metabolic, Capsaicin. Moreover, capsaicin also serves as defense mechanism, antiarthritis, analgesic, and anticancer. This study aimed to isolate Acyltransferase (AT3) gene which encoding Capsaicin Synthase (CS) enzyme. AT3 gene was isolated through PCR using forward primer 5’-ATG GCT TTT GCA TTA CCA TCA-3’ and reverse primer 5’-CCT TCA CAA TTA TTC GCC CA-3’. Data were analyzed using DNA Baser, BLAST, and ClustalX. This study has successfully isolated 404 bp fragments of AT3 gene. This fragments located at 1918-1434 bp referred to AT3 gene from Capsicum frutescens cv. Shuanla. Isolation of upstream and downstream fragments of AT3 gene from Capsicum frutescens cv. Cakra Hijau is undergoing.

Poeciliidae family consists of small live-bearer fishes with sexual dimorphic. This fishes are widely distributed across Indonesian archipelago, but biological studies on this fish are very limited. This research is aimed to identify the species of Peciliidae fishes which lives in Sari Lake Pasuruan based on gonopodium structures and morphometric analysis. A specific characteristic of the Poeciliidae family is its gonopodium structures which is a modification of 3rd, 4th, and 5th rays of the male’s anal fin. Each species represented 50 samples male and female from each type we found and measured 26 characters (in mm). Cladogram based on morphometric measurements showed that sample 1 and 2 had 100% similarity, sample 3 had 94% similarity with sample 1 and 2. Sample 4 had 54% similarity with sample 1, 2, and 3. Based on gonopodium structures sample 1 and sample 2 included Xiphophorus hellerii Heckel possess large claw on the terminus of ray 5 hook is small crescent-moon shaped on 3rd ray and lacking of gonopodium palp, sample 3 possesses distal platform with 11 retrosse serrae,and membranous hook so we were able to identify the sample 3 as Poecilia mexicana Steindachner, whilst sample 4 was Poecilia reticulata Peter had a long-pointed comb-like spines on ray 3rd and and has a small hook on the tip of 5p ray.

Frog is an amphibian which is widely spread around the world. Indonesia houses 450 species which represent 11% of frog species in the world. In Java Island alone, there live 42 species of frogs and toads. Frogs can be used as an environment indicator in that the presence of frog in a particular place indicates that the place stays natural and unpolluted. The 1st Campus of State University of Malang, which is located in the heart of Malang District, has been developing rapidly currently. Thus, it requires the construction of new various facilities to support its huge activities. Extensive construction can be destructive even damaging to the habitat of frog, which potentially threats the frog’s life, if it does not take the environmental impact into careful consideration. This study is aimed to identify the species of frog which survives at State University of Malang with, particularly the frog species found in 1995. Species identification was conducted by observing the morphological character. This study found that there were four species with three species remained survived in 1995; those were Duttaphrynus melanostictus, Polypedates leucomystax, and Kaloula baleta; and one new species called Rana chalconota. This study also revealed that there were four species which were extinct; those were Fejervarya cancrivora, Fejervarya limnocharis, Ingerophrynus biporcatus, and Occidoziga lima. This situation shows the decreasing amount of species from 7 to 4 within the last 17 years. This result indicates that there is a serious environmental degradation which causes the losing of frog habitats. Further research is needed to study the ecological condition changing in order to save the frog species.

Tree frogs live in natural places which are unpolluted. Regarding their role as an ecological indicator, the decrease of frogs population in a particular habitat indicates the danger of environment quality decrease. Moreover, this condition can harm the frogs themselves. All kinds of frogs breed in aqueous environment such as ponds, marshes, and farming fields. One of the tree frogs, Polypedates leucomystax, which belongs to Familia Rachophoridae, is widely spread in Indonesia. This frog has yellowish brown skin with black spots or six lines extending from head to the posterior tip of body. A breeding couple of the frog produces foam nests on the water or plants around water body, where they will nest their fertilized eggs. This species produces over a hundred embryos in one spawning season. These embryos require appropriate conditions to develop normally in the nature. Frog embryo development may becomes a reference to understand how the frog population survives. This study focused on P. leucomystax with regards to its decrease in number due to the drying up of the environment and a lot lost of trees in Campus of State University of Malang. The development of P. leucomystax embryos in the reproduction foam was observed until it reached a tadpole stage. The result showed that the embryos developed in the foam until they hatched then they move out of the foam into the water under which they would continue their development. Considering that water body is a critical requirement for the development of P. leucomystax embryos, it is our responsibility to make any efforts to conserve not only the trees but also any type of water bodies including ponds, marshes, and farming fields as well.

Hylarana chalconota is a cryptic species of frog endemic to Java Island [1]. This species is small with long legs, and brown skin. The Snout-Vent Length (SVL) ranges between 30-40 mm for male and 45-65 mm for female. [4] Reports the existence of this species in State University of Malang, which was not found in 1995 [5]. Sampel #1 displays spots in its skin, which does not exist in sample #2. To reveal the haplotype diversity of COI gene in this species, we analyzed Cytochrome-c oxidase subunit-1 (COI) sequences of both samples. Using a pair of primers according to [6] both samples had 604 bp and 574 bp fragment length, respectively. These fragments showed polymorphism; with mutation position in sites 104, 105, and 124. Based on this result, we suggest that the two samples share a different haplotypes, proposed as UM1 and UM2.