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12. Oral human papillomavirus infection in England and associated risk factors: a case-control study

Author

Hearnden, V., Murdoch, C., D'Apice, K., Duthie, S., Hayward, N.J., and Powers, H.J.

Subjects
stomatognathic diseases
Abstract

OBJECTIVES: This study was conducted to determine the prevalence of and associated risk factors for infection with oral high-risk human papillomavirus (HR-HPV) in adult participants within England, and to explore any association with oral mucosal buccal epithelial cell and whole blood folate concentration. DESIGN: This was an observational study to determine oral HR-HPV prevalence in the study population. A case-control study was performed to explore the association between infection and folate status. SETTING: This study was conducted in Sheffield, UK, between April 2013 and August 2014. PARTICIPANTS: Seven hundred participants, aged 18-60 years, were recruited from university students (n=179), university and hospital staff (n=163), dental hospital patients (n=13), Sexual Health Sheffield patients (n=122) and the general public (n=223). INTERVENTIONS: Participants completed a lifestyle and sexual behaviour questionnaire, provided an oral rinse and gargle sample for the detection of oral HR-HPV and an oral mucosal buccal epithelial cell sample for the measurement of oral mucosal buccal epithelial cell folate. A blood sample was collected for measurement of whole blood folate concentration. OUTCOME MEASURES: The prevalence of oral HR-HPV infection in the study population was the primary outcome measure. Secondary outcome measures included associations between risk factors, folate status and infection. RESULTS: The prevalence of oral HR-HPV infection in this cohort was 2.2% (15/680) with 0.7% (5/680) positive for HPV16 or HPV18. Twenty samples were excluded due to insufficient material for HPV detection. Participants with oral HR-HPV infection were more likely to be a former smoker, and have a greater number of sexual and oral sexual partners. Folate status was not linked to likelihood of HPV infection. CONCLUSIONS: The prevalence of oral infection with HR-HPV in adult men and women in Sheffield in the North of England was low. Smoking and sexual behaviour were associated with HR-HPV positivity. TRIAL REGISTRATION NUMBER: ID14106.

Published
2018

16. The NuGO proof of principle study package: a collaborative research effort of the European Nutrigenomics Organisation

Author

Baccini, M, Bachmaier, EM, Biggeri, A, Boekschoten, MV, Bouwman, FG, Brennan, L, Caesar, R, Cinti, S, Coort, SL, Crosley, K, Daniel, H, Drevon, CA, Duthie, S, Eijssen, L, Elliott, RM, van Erk, M, Evelo, C, Gibney, M, Heim, C, Horgan, GW, Johnson, IT, Kelder, T, Kleemann, R, Kooistra, T, van Iersel, MP, Mariman, EC, Mayer, C, McLoughlin, G, Müller, M, Mulholland, F, van Ommen, B, Polley, AC, Pujos-Guillot, E, Rubio-Aliaga, I, Roche, HM, de Roos, B, Sailer, M, Tonini, G, Williams, LM, de Wit, N, For the NuGO PPS Team, Università degli Studi di Firenze = University of Florence (UniFI), University of Aberdeen, Division of Human Nutrition, Wageningen University and Research [Wageningen] (WUR), Top Institute Food and Nutrition (TIFN), Maastricht University [Maastricht], School of Agriculture, Food Science and Veterinary Medicine, University College Dublin [Dublin] (UCD), University of Oslo (UiO), Università Politecnica delle Marche, Partenaires INRAE, Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM), Institute of Food Research [Norwich], Biotechnology and Biological Sciences Research Council (BBSRC), Department of Physiological Genomics, Munich University, University College Dublin (UCD), Netherlands Organisation for Applied Scientific Research, Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université, TNO Kwaliteit van Leven, Università degli Studi di Firenze = University of Florence [Firenze] (UNIFI), Humane Biologie, Bioinformatica, RS: CARIM School for Cardiovascular Diseases, and RS: NUTRIM - R4 - Gene-environment interaction

Subjects
Endocrinology, Diabetes and Metabolism, [SDV]Life Sciences [q-bio], MEDLINE, 030209 endocrinology & metabolism, Physiological Sciences, Bioinformatics, 03 medical and health sciences, Voeding, Metabolisme en Genomica, 0302 clinical medicine, Voeding, Genetics, Network of excellence, Medicine, 030304 developmental biology, Nutrition, VLAG, 0303 health sciences, business.industry, Metabolism and Genomics, Nutrigenomics, immune-system, Proof of concept, Metabolisme en Genomica, Commentary, Engineering ethics, Nutrition, Metabolism and Genomics, business
Abstract

Acknowledgments This project is funded by the Nutrigenomics Organisation, EC funded Network of Excellence, grant nr.FOOD- 2004-506360.

Published
2008

19. Transcriptome analysis of peripheral blood mononuclear cells in human subjects following a 36 h fast provides evidence of effects on genes regulating inflammation, apoptosis and energy metabolism

Author

Elliott, R. M., primary, de Roos, B., additional, Duthie, S. J., additional, Bouwman, F. G., additional, Rubio-Aliaga, I., additional, Crosley, L. K., additional, Mayer, C., additional, Polley, A. C., additional, Heim, C., additional, Coort, S. L., additional, Evelo, C. T., additional, Mulholland, F., additional, Daniel, H., additional, Mariman, E. C., additional, and Johnson, I. T., additional

Published
2014
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20. The NuGO proof of principle study package: a collaborative research effort of the European Nutrigenomics Oganisation

Author

Baccini, M., Bachmaier, E.M., Biggeri, A., Boekschoten, M.V., Bouwman, F.G., Brennan, L., Caesar, R., Cinti, S., Coort, S.L., Crosley, K., Daniel, H., Drevon, C.A., Duthie, S., Eijssen, L., Elliott, R., van Erk, M.J., Evelo, C., Gibney, M.J., Heim, C., Horgan, G., Johnson, I.T., Kelder, T., Kleemann, R., Kooistra, T., van Iersel, M.P., Mariman, E.C.M., Mayer, C., McLoughlin, G., Müller, M.R., Mulholland, F., van Ommen, B., Polley, A.C., Pujos-Guillot, E., Rubio-Aliaga, I., Roche, H., de Roos, B., Sailer, M., Tonini, G., Williams, L.M., de Wit, N.J.W., Baccini, M., Bachmaier, E.M., Biggeri, A., Boekschoten, M.V., Bouwman, F.G., Brennan, L., Caesar, R., Cinti, S., Coort, S.L., Crosley, K., Daniel, H., Drevon, C.A., Duthie, S., Eijssen, L., Elliott, R., van Erk, M.J., Evelo, C., Gibney, M.J., Heim, C., Horgan, G., Johnson, I.T., Kelder, T., Kleemann, R., Kooistra, T., van Iersel, M.P., Mariman, E.C.M., Mayer, C., McLoughlin, G., Müller, M.R., Mulholland, F., van Ommen, B., Polley, A.C., Pujos-Guillot, E., Rubio-Aliaga, I., Roche, H., de Roos, B., Sailer, M., Tonini, G., Williams, L.M., and de Wit, N.J.W.

Published
2008

21. Automated cycle sequencing with Taquenase: protocols for internal labeling, dye primer and 'doublex' simultaneous sequencing

Author

Voss H, Nentwich U, Duthie S, Stefan Wiemann, Benes V, Zimmermann J, and Ansorge W

Subjects
Nucleotides, RNA-Directed DNA Polymerase, Taq Polymerase, DNA, DNA-Directed DNA Polymerase, Sequence Analysis, DNA, Polymerase Chain Reaction, DNA Primers, Fluorescent Dyes
Abstract

This paper describes automated cycle sequencing protocols for internal labeling, dye primer and "doublex" simultaneous sequencing using Taquenase, a new genetically modified DNA polymerase with increased thermostability. Sequencing performance both with labeled and unlabeled primer yields uniform unambiguous signals up to the resolution limit of the sequencing gels. Primer walking with internal labeling was successfully performed on Pl-derived artificial chromosome (PAC) constructs with 130-kb inserts. Taquenase, a commercially available modified thermostable sequencing enzyme (delta 280, F667Y Taq DNA polymerase), incorporates a variety of fluorescent dNTPs carrying fluorescein isothiocyanate, TexasRed or Cy5 labels during the cycle-sequencing process with higher efficiency than other thermostable DNA polymerases. Comparison to other modified Taq DNA polymerases suggests that the particular N-terminal deletion of Taquenase rather than the presence of the F667Y mutation is responsible for the efficient incorporation and extension of labeled dNTPs. Taquenase makes feasible highly accurate "doublex" simultaneous cylce sequencing on both strands of template DNA with two internal labels or two dye-labeled primers in combination with the EMBL-2-dye DNA sequencing system, ARAKIS, or with two commercial DNA sequencers. It allows up to 2000 bases at99% accuracy to be determined in a single reaction.

Published
1997

22. FROM MARGINAL TO MAINSTREAM: AN ARGUMENT FOR LOCATING EDUCATION DEVELOPMENT IN THE ACADEMIC DEPARTMENT.

Author

Duthie, S. R. and Freeman, L.

Subjects
SOCIAL stigma, EXTENDED teacher education programs, UNIVERSITIES & colleges, RESPONSIBILITY
Abstract

This article contends that core responsibility, formation and execution of education development (eD) initiatives should occur in the academic department. For too long, eD has been handed off to peripheral bodies within South African universities. these programmes are limited in their reach, relevance and usefulness to students. through reporting on a case study of eD in the Department of Political Studies at the University of Cape town (UCt), Cape town, South Africa, the authors argue that eD within the academic discipline has three major benefits, namely: (i) initiatives can develop based on effective identification of student need; (ii) ED can be normalised and its stigma can be reduced; and (iii) ED becomes discipline-specific and properly contextualised to student learning. [ABSTRACT FROM AUTHOR]

Published
2015

31. An automated biochemical assay for haptoglobin: prevention of interference from albumin

Author

Eckersall, P.D., Duthie, S., Safi, S., Moffatt, D., Horadagoda, N.U., Doyle, Sean, Parton, R., Bennett, D., Fitzpatrick, J.L., Eckersall, P.D., Duthie, S., Safi, S., Moffatt, D., Horadagoda, N.U., Doyle, Sean, Parton, R., Bennett, D., and Fitzpatrick, J.L.

Abstract

Measurement of the acute phase serum protein, haptoglobin (Hp), is performed by biochemical methods based on haemoglobin binding, in many veterinary diagnostic laboratories. During attempts to develop a robust biochemical assay for serum Hp it was discovered that serum albumin interfered with the assay system increasing results by as much as 0.28 mg/ml, which could affect interpretation of results especially in species with low normal Hp concentrations. A reagent cocktail (SB-7) was devised which inhibited the interfering effect of albumin. An automated assay for Hp utilising SB-7 was developed for production as a biochemical assay kit and was evaluated for use in veterinary diagnosis. The intra-assay coefficients of variation were of 0.9%, 0.9% and 1.3% for Hp concentrations of 2.0, 1.0 and 0.23 mg/ml, respectively and interassay coefficients of variation of 1.7% and 4.5% for Hp of 2.08 mg/ml and 0.24 mg/ml, respectively. The lower limit of detection of was 0.02 mg/ml, linearity extended to 8 mg/ml and recovery was 101±7% (mean ±SD). The assay had correlation coefficients (R2) of 0.96 and 0.90 when compared with immunodiffusion assays of canine Hp and bovine Hp, respectively. Lipaemia and bilirubinaemia caused no interference. Haemolysis did not affect measurement of low levels of Hp, but at serum Hp concentrations of 0.4 and 1.8 mg/ml the apparent Hp concentration was decreased. Elevated concentrations of Hp were measured in cattle with mastitis, dogs with polyarthritis and rats experimentally infected withBordetella pertussis. The automated assay is precise and has negligible interference from albumin.

Published
1999

39. Uniform Band Intensities in Fluorescent Dye Terminator Sequencing

Author

Kumar, Shiv, primary, Fuller, C. W., additional, Nampalli, S., additional, Khot, M., additional, Livshin, I., additional, Sun, L., additional, Hamilton, S., additional, Samols, S. B., additional, Mamone, J. A., additional, Hujer, K. M., additional, McArdle, B. F., additional, Nelson, J. R., additional, and Duthie, S., additional

Published
1999
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43. Characterization of Genes Encoding Translation Initiation Factor eIF-2 in Mouse and Human: Sex Chromosome Localization, Escape from X-Inactivation and Evolution

Author

Ehrmann, I. E., primary, Ellis, P. S., additional, Mazeyrat, S., additional, Duthie, S., additional, Brockdorff, N., additional, Mattei, M. G., additional, Gavin, M. A., additional, Affara, N. A., additional, Brown, G. M., additional, Simpson, E., additional, Mitchell, M. J., additional, and Scott, D. M., additional

Published
1998
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