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Abstract Background Hematophagy is a common trait of insect vectors of disease. Extensive genome-wide transcriptional changes occur in mosquitoes after blood meals, and these are related to digestive and reproductive processes, among others. Studies of these changes are expected to reveal molecular targets for novel vector control and pathogen transmission-blocking strategies. The mosquito Aedes aegypti (Diptera, Culicidae), a vector of Dengue viruses, Yellow Fever Virus (YFV) and Chikungunya virus (CV), is the subject of this study to look at genome-wide changes in gene expression following a blood meal. Results Transcriptional changes that follow a blood meal in Ae. aegypti females were explored using RNA-seq technology. Over 30% of more than 18,000 investigated transcripts accumulate differentially in mosquitoes at five hours after a blood meal when compared to those fed only on sugar. Forty transcripts accumulate only in blood-fed mosquitoes. The list of regulated transcripts correlates with an enhancement of digestive activity and a suppression of environmental stimuli perception and innate immunity. The alignment of more than 65 million high-quality short reads to the Ae. aegypti reference genome permitted the refinement of the current annotation of transcript boundaries, as well as the discovery of novel transcripts, exons and splicing variants. Cis-regulatory elements (CRE) and cis-regulatory modules (CRM) enriched significantly at the 5'end flanking sequences of blood meal-regulated genes were identified. Conclusions This study provides the first global view of the changes in transcript accumulation elicited by a blood meal in Ae. aegypti females. This information permitted the identification of classes of potentially co-regulated genes and a description of biochemical and physiological events that occur immediately after blood feeding. The data presented here serve as a basis for novel vector control and pathogen transmission-blocking strategies including those in which the vectors are modified genetically to express anti-pathogen effector molecules.

Abstract Background The most abundant family of insect cuticular proteins, the CPR family, is recognized by the R&R Consensus, a domain of about 64 amino acids that binds to chitin and is present throughout arthropods. Several species have now been shown to have more than 100 CPR genes, inviting speculation as to the functional importance of this large number and diversity. Results We have identified 156 genes in Anopheles gambiae that code for putative cuticular proteins in this CPR family, over 1% of the total number of predicted genes in this species. Annotation was verified using several criteria including identification of TATA boxes, INRs, and DPEs plus support from proteomic and gene expression analyses. Two previously recognized CPR classes, RR-1 and RR-2, form separate, well-supported clades with the exception of a small set of genes with long branches whose relationships are poorly resolved. Several of these outliers have clear orthologs in other species. Although both clades are under purifying selection, the RR-1 variant of the R&R Consensus is evolving at twice the rate of the RR-2 variant and is structurally more labile. In contrast, the regions flanking the R&R Consensus have diversified in amino-acid composition to a much greater extent in RR-2 genes compared with RR-1 genes. Many genes are found in compact tandem arrays that may include similar or dissimilar genes but always include just one of the two classes. Tandem arrays of RR-2 genes frequently contain subsets of genes coding for highly similar proteins (sequence clusters). Properties of the proteins indicated that each cluster may serve a distinct function in the cuticle. Conclusion The complete annotation of this large gene family provides insight on the mechanisms of gene family evolution and clues about the need for so many CPR genes. These data also should assist annotation of other Anopheles genes.

The magnetospheric cusp connects the planetary magnetic field to interplanetary space, offering opportunities for charged particles to precipitate to or escape from the planet. Terrestrial cusps are typically found near noon local time, but the characteristics of the Jovian cusp are unknown. Here we show direct evidence of Jovian cusps using datasets from multiple instruments onboard Juno spacecraft. We find that the cusps of Jupiter are in the dusk sector, which is contradicting Earth-based predictions of a near-noon location. Nevertheless, the characteristics of charged particles in the Jovian cusps resemble terrestrial and Saturnian cusps, implying similar cusp microphysics exist across different planets. These results demonstrate that while the basic physical processes may operate similarly to those at Earth, Jupiter's rapid rotation and its location in the heliosphere can dramatically change the configuration of the cusp. This work provides useful insights into the fundamental consequences of star-planet interactions, highlighting how planetary environments and rotational dynamics influence magnetospheric structures. Jovian cusps are not well-known due to limited observations. Here, the authors show that the characteristics of charged particles in the Jovian cusps are similar to those of the Earth and Saturn cusps, and Jupiter's polar cusp is located in the dusk-side sector, contradicting Earth-based predictions of a near-noon location. [ABSTRACT FROM AUTHOR]

The Jovian magnetosphere is highly dynamic, influenced by both solar wind and internal processes associated with the rapid planetary rotation and Io's volcanic activities. Accompanying the mass and energy circulations driven by the magnetospheric dynamics, the magnetic configuration also changes dramatically. One of the crucial parameters to characterize the magnetic configuration is magnetic field line curvature (FLC), which generally describes how stretched the field line is. The curvature is pivotal to influence particle behaviors, for example, pitch angle scattering which may lead to auroral particle precipitation. In this work, a method is proposed to investigate the real‐time magnetic FLC in Jovian current sheet using the magnetic field data from the Juno spacecraft. The results indicate that the FLC scattering of ions and relativistic electrons are common in Jovian magnetosphere, providing a crucial insight to understand the particle behaviors. Plain Language Summary: Both the Earth and the Jupiter have intrinsic magnetic field. When the planetary magnetic field interacts with the solar wind, a region called magnetosphere is formed. Particle behaviors in different planetary systems are different, due to the different magnetospheric dynamics. The curvature of magnetic field, describing the stretch level of a magnetic field line, is a basic parameter to describe a planetary space system, and it can significantly influence particle behaviors, for example, to scatter the magnetospheric particles to planetary atmosphere, causing auroral emissions. In this work, we proposed a method to calculate the magnetic field line curvature (FLC) near the equatorial plane inside the Jupiter's magnetosphere using Juno data set, for the first time to provide a global picture on the magnetic FLC. By comparing with the radius of particles' gyration motions, we suggest that ions and electrons can be strongly scattered by the magnetic FLC. We believe that the results in this study provide useful information on the different particle behaviors between the terrestrial system and the Jovian system. Key Points: We proposed a method to investigate the magnetic field line curvature (FLC) in Jupiter's current sheet using data from Juno data set50 events are selected by specific criteria. The magnetic FLC and different particles' Larmor radius are investigatedThe FLC will scatter ions and relativistic electrons as a potential cause of auroral precipitation [ABSTRACT FROM AUTHOR]

BackgroundHematophagy is a common trait of insect vectors of disease. Extensive genome-wide transcriptional changes occur in mosquitoes after blood meals, and these are related to digestive and reproductive processes, among others. Studies of these changes are expected to reveal molecular targets for novel vector control and pathogen transmission-blocking strategies. The mosquito Aedes aegypti (Diptera, Culicidae), a vector of Dengue viruses, Yellow Fever Virus (YFV) and Chikungunya virus (CV), is the subject of this study to look at genome-wide changes in gene expression following a blood meal.ResultsTranscriptional changes that follow a blood meal in Ae. aegypti females were explored using RNA-seq technology. Over 30% of more than 18,000 investigated transcripts accumulate differentially in mosquitoes at five hours after a blood meal when compared to those fed only on sugar. Forty transcripts accumulate only in blood-fed mosquitoes. The list of regulated transcripts correlates with an enhancement of digestive activity and a suppression of environmental stimuli perception and innate immunity. The alignment of more than 65 million high-quality short reads to the Ae. aegypti reference genome permitted the refinement of the current annotation of transcript boundaries, as well as the discovery of novel transcripts, exons and splicing variants. Cis-regulatory elements (CRE) and cis-regulatory modules (CRM) enriched significantly at the 5'end flanking sequences of blood meal-regulated genes were identified.ConclusionsThis study provides the first global view of the changes in transcript accumulation elicited by a blood meal in Ae. aegypti females. This information permitted the identification of classes of potentially co-regulated genes and a description of biochemical and physiological events that occur immediately after blood feeding. The data presented here serve as a basis for novel vector control and pathogen transmission-blocking strategies including those in which the vectors are modified genetically to express anti-pathogen effector molecules.

On 15 September 2021, Chandra carried out a 40‐hr (∼4 jovian rotations) observation as part of its longest planetary campaign to study the drivers of jovian X‐ray aurora that may be linked to ultra‐low frequency (ULF) wave activity. During this time, Juno's orbit had taken the spacecraft into Jupiter's dusk magnetosphere. Here is believed to be the most probable location of ULF waves propagating along jovian magnetic field lines that drive the X‐ray auroral emissions. This is the first time that this region has been observed by an orbiter since Galileo >20 years ago, and never before has there been contemporaneous in situ and X‐ray observations. A 1D solar wind propagation model identifies a compression event near the midpoint of the 40‐hr observation window. The influence of a compression is confirmed when comparing the measured magnetic field in the dusk lobes of the magnetotail from Juno MAG data against a baseline lobe field model. Data from the Juno Waves instrument also show activation of broadband kilometric (bKOM) emissions during the arrival of the shock, a feature that has previously been observed during compression events. Therefore this is the first time we can fully analyze the morphological variability during the evolution of a shock. Wavelet transforms and Rayleigh testing are used to search for statistically significant quasi‐periodic pulsations (QPPs) of the X‐ray emissions in the data set, and find significant QPPs with periods of 25–26 min for the northern auroral X‐rays. Key Points: We compare a 40‐hr Chandra observation of Jupiter's X‐ray aurora with in situ Juno measurements and a 1‐D solar wind propagation modelWe find statistically significant quasi‐periodic pulsation with a ∼25 min period likely linked to the arrival of a solar wind compressionUsing Juno MAG data form the dusk tail lobes, we infer the state of compression/loading of the magnetosphere [ABSTRACT FROM AUTHOR]

Magnetic Dipolarization, a hallmark indicator of the formation of field‐aligned currents, plays a crucial role in the energy dissipative processes that occur within planetary magnetospheres. Saturn's magnetic dipolarization was found to recur after one planetary rotation, suggesting a potential correlation with the corotating current systems. The observation of enhanced Energetic Neutral Atoms (ENA) within Saturn's inner magnetosphere serves as a diagnostic tool, revealing the existence of a rotating, dynamic Partial Ring Current (PRC) system. By utilizing multiple datasets from Cassini, this study advances the understanding of the interrelationship between ENA emission and magnetic dipolarization. Our results suggest the possibility of a coupling between the PRC system revealed by the ENA emission and magnetic dipolarization. Furthermore, a temporal correlation was found between the ENA emissions and two distinct radio emissions. This study discusses potential causal relationships among these phenomena and first time proposes a global unified physical picture. Plain Language Summary: Energy dissipation and circulation are the core of the space plasma field, which shows different typical features at different planets. Unlike the solar wind‐driven terrestrial magnetosphere, the giant planetary magnetospheric processes are strongly controlled by internal processes, and show strong rotational modulation in the in situ and remote sensing measurements. The rotation modulation of the observations in Saturn's magnetosphere may lead to challenges in the interpretation of data. The observed variations in data are a combination of spatial and temporal variations. In this study, we analyzed the multiple datasets from Cassini to formulate a uniform picture of the periodic variations including magnetic field variation, rotating hot plasma cloud, and radio emissions. We propose that the corotating current system could potentially connect all observed phenomena. Key Points: Recurrent magnetic dipolarization and Energetic Neutral Atoms (ENA) enhancement are correlatedThe SKR and 20 kHz narrow band emissions are likely connected to the rotating hot plasma cloud revealed by ENA observationPeriodic enhancements of SKR are concurrent but slightly time‐lag/lead the recurrent magnetic dipolarizations [ABSTRACT FROM AUTHOR]