Search

Your search keyword '"Dunham, Wade H."' showing total 14 results
Start Over Author "Dunham, Wade H."
14 results on '"Dunham, Wade H."'

3. Crystal structures and mutagenesis of PPP-family ser/thr protein phosphatases elucidate the selectivity of cantharidin and novel norcantharidin-based inhibitors of PP5C

Author

Chattopadhyay, Debasish, Swingle, Mark R., Salter, Edward A., Wood, Eric, D’Arcy, Brandon, Zivanov, Catherine, Abney, Kevin, Musiyenko, Alla, Rusin, Scott F., Kettenbach, Arminja, Yet, Larry, Schroeder, Chad E., Golden, Jennifer E., Dunham, Wade H., Gingras, Anne-Claude, Banerjee, Surajit, Forbes, David, Wierzbicki, Andrzej, and Honkanen, Richard E.

Published
2016
Full Text
View/download PDF

6. The linear ubiquitin-specific deubiquitinase gumby regulates angiogenesis

Author

Rivkin, Elena, Almeida, Stephanie M., Ceccarelli, Derek F., Juang, Yu-Chi, MacLean, Teresa A., Srikumar, Tharan, Huang, Hao, Dunham, Wade H., Fukumura, Ryutaro, Xie, Gang, Gondo, Yoichi, Raught, Brian, Gingras, Anne-Claude, Sicheri, Frank, and Cordes, Sabine P.

Subjects
Gene mutations -- Testing, Ubiquitin-proteasome system -- Research, Cellular signal transduction -- Observations, Wnt proteins -- Properties, Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

A complex interaction of signalling events, including the Wnt pathway, regulates sprouting of blood vessels from pre-existing vasculature during angiogenesis. Here we show that two distinct mutations in the (uro)chordate-specific gumby (also called Fam105b) gene cause an embryonic angiogenic phenotype in gumby mice. Gumby interacts with disheveled 2 (DVL2), is expressed in canonical Wnt-responsive endothelial cells and encodes an ovarian tumour domain class of deubiquitinase that specifically cleaves linear ubiquitin linkages. A crystal structure of gumby in complex with linear diubiquitin reveals how the identified mutations adversely affect substrate binding and catalytic function in line with the severity of their angiogenic phenotypes. Gumby interacts with HOIP (also called RNF31), a key component of the linear ubiquitin assembly complex, and decreases linear ubiquitination and activation of NF-κB-dependent transcription. This work provides support for the biological importance of linear (de)ubiquitination in angiogenesis, craniofacial and neural development and in modulating Wnt signalling., During angiogenesis, new blood vessels sprout from pre-existing vasculature to form a microcapillary network and become uniquely adapted to the physiology and function of the organs that they infiltrate (reviewed [...]

Published
2013
Full Text
View/download PDF

7. The NEMP family supports metazoan fertility and nuclear envelope stiffness

Author

Tsatskis, Yonit, primary, Rosenfeld, Robyn, additional, Pearson, Joel D., additional, Boswell, Curtis, additional, Qu, Yi, additional, Kim, Kyunga, additional, Fabian, Lacramioara, additional, Mohammad, Ariz, additional, Wang, Xian, additional, Robson, Michael I., additional, Krchma, Karen, additional, Wu, Jun, additional, Gonçalves, João, additional, Hodzic, Didier, additional, Wu, Shu, additional, Potter, Daniel, additional, Pelletier, Laurence, additional, Dunham, Wade H., additional, Gingras, Anne-Claude, additional, Sun, Yu, additional, Meng, Jin, additional, Godt, Dorothea, additional, Schedl, Tim, additional, Ciruna, Brian, additional, Choi, Kyunghee, additional, Perry, John R. B., additional, Bremner, Rod, additional, Schirmer, Eric C., additional, Brill, Julie A., additional, Jurisicova, Andrea, additional, and McNeill, Helen, additional

Published
2020
Full Text
View/download PDF

9. Protein Interaction Network of the Mammalian Hippo Pathway Reveals Mechanisms of Kinase-Phosphatase Interactions

Author

Couzens, Amber L., primary, Knight, James D. R., additional, Kean, Michelle J., additional, Teo, Guoci, additional, Weiss, Alexander, additional, Dunham, Wade H., additional, Lin, Zhen-Yuan, additional, Bagshaw, Richard D., additional, Sicheri, Frank, additional, Pawson, Tony, additional, Wrana, Jeffrey L., additional, Choi, Hyungwon, additional, and Gingras, Anne-Claude, additional

Published
2013
Full Text
View/download PDF

10. The CRAPome: a contaminant repository for affinity purification–mass spectrometry data

Author

Mellacheruvu, Dattatreya, primary, Wright, Zachary, additional, Couzens, Amber L, additional, Lambert, Jean-Philippe, additional, St-Denis, Nicole A, additional, Li, Tuo, additional, Miteva, Yana V, additional, Hauri, Simon, additional, Sardiu, Mihaela E, additional, Low, Teck Yew, additional, Halim, Vincentius A, additional, Bagshaw, Richard D, additional, Hubner, Nina C, additional, al-Hakim, Abdallah, additional, Bouchard, Annie, additional, Faubert, Denis, additional, Fermin, Damian, additional, Dunham, Wade H, additional, Goudreault, Marilyn, additional, Lin, Zhen-Yuan, additional, Badillo, Beatriz Gonzalez, additional, Pawson, Tony, additional, Durocher, Daniel, additional, Coulombe, Benoit, additional, Aebersold, Ruedi, additional, Superti-Furga, Giulio, additional, Colinge, Jacques, additional, Heck, Albert J R, additional, Choi, Hyungwon, additional, Gstaiger, Matthias, additional, Mohammed, Shabaz, additional, Cristea, Ileana M, additional, Bennett, Keiryn L, additional, Washburn, Mike P, additional, Raught, Brian, additional, Ewing, Rob M, additional, Gingras, Anne-Claude, additional, and Nesvizhskii, Alexey I, additional

Published
2013
Full Text
View/download PDF

13. BioID-based Identification of Skp Cullin F-box (SCF)β-TrCP1/2E3 Ligase Substrates*[S]

Author

Coyaud, Etienne, Mis, Monika, Laurent, Estelle M.N., Dunham, Wade H., Couzens, Amber L., Robitaille, Melanie, Gingras, Anne-Claude, Angers, Stephane, and Raught, Brian

Abstract

The identification of ubiquitin E3 ligase substrates has been challenging, due in part to low-affinity, transient interactions, the rapid degradation of targets and the inability to identify proteins from poorly soluble cellular compartments. SCFβ-TrCP1and SCFβ-TrCP2are well-studied ubiquitin E3 ligases that target substrates for proteasomal degradation, and play important roles in Wnt, Hippo, and NFκB signaling. Combining 26S proteasome inhibitor (MG132) treatment with proximity-dependent biotin labeling (BioID) and semiquantitative mass spectrometry, here we identify SCFβ-TrCP1/2interacting partners. Based on their enrichment in the presence of MG132, our data identify over 50 new putative SCFβ-TrCP1/2substrates. We validate 12 of these new substrates and reveal previously unsuspected roles for β-TrCP in the maintenance of nuclear membrane integrity, processing (P)-body turnover and translational control. Together, our data suggest that β-TrCP is an important hub in the cellular stress response. The technique presented here represents a complementary approach to more standard IP-MS methods and should be broadly applicable for the identification of substrates for many ubiquitin E3 ligases.

Published
2015
Full Text
View/download PDF

14. The NEMP family supports metazoan fertility and nuclear envelope stiffness.

Author

Yonit Tsatskis, Rosenfeld, Robyn, Pearson, Joel D., Boswell, Curtis, Yi Qu, Kyunga Kim, Fabian, Lacramioara, Mohammad, Ariz, Xian Wang, Robson, Michael I., Krchma, Karen, Jun Wu, Gonçalves, João, Hodzic, Didier, Shu Wu, Potter, Daniel, Pelletier, Laurence, Dunham, Wade H., Gingras, Anne-Claude, and Yu Sun

Subjects
*NUCLEAR membranes, *MEIOSIS, *GERMINAL vesicles, *STEM cell niches, *FERTILITY, *LYSIS, *DEVELOPMENTAL biology
Abstract

The article offers information on nuclear envelope membrane protein 1 (NEMP) family supports metazoan fertility and nuclear envelope stiffness. It mentions multi-transmembrane nuclear envelope (NE) protein with homologs found in all metazoans; No genetic models exist for Nemp function in any system; and Super-resolution microscopy revealed that dNemp localizes to inner nuclear membranes (INMs).

Published
2020
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results