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158 results on '"Dumler, Inna"'

30. Urokinase receptor counteracts vascular smooth muscle cell functional changes induced by surface topography

Author

Kiyan, Yulia, Kurselis, Kestutis, Kiyan, Roman, Haller, Hermann, Chichkov, Boris, Dumler, Inna, Kiyan, Yulia, Kurselis, Kestutis, Kiyan, Roman, Haller, Hermann, Chichkov, Boris, and Dumler, Inna

Abstract

Molecular beacons (MBs) of DCurrent treatments for human coronary artery disease necessitate the development of the next generations of vascular bioimplants. Recent reports provide evidence that controlling cell orientation and morphology through topographical patterning might be beneficial for bioimplants and tissue engineering scaffolds. However, a concise understanding of cellular events underlying cell-biomaterial interaction remains missing. In this study, applying methods of laser material processing, we aimed to obtain useful markers to guide in the choice of better vascular biomaterials. Our data show that topographically treated human primary vascular smooth muscle cells (VSMC) have a distinct differentiation profile. In particular, cultivation of VSMC on the microgrooved biocompatible polymer E-shell induces VSMC modulation from synthetic to contractile phenotype and directs formation and maintaining of cell-cell communication and adhesion structures. We show that the urokinase receptor (uPAR) interferes with VSMC behavior on microstructured surfaces and serves as a critical regulator of VSMC functional fate. Our findings suggest that microtopography of the E-shell polymer could be important in determining VSMC phenotype and cytoskeleton organization. They further suggest uPAR as a useful target in the development of predictive models for clinical VSMC phenotyping on functional advanced biomaterials.NA and RNA have aroused increasing interest because they allow a continuous readout, excellent spatial and temporal resolution to observe in real time. This kind of dual-labeled oligonucleotide probes can differentiate between bound and unbound DNA/RNA in homogenous hybridization with a high signal-to-background ratio in living cells. This review briefly summarizes the different unnatural sugar backbones of oligonucleotides combined with fluoro-phores that have been employed to sense DNA/RNA. With different probes, we epitomize the fundamental understanding of

Published
2013

47. Induction of c-fos Gene Expression by Urokinase-Type Plasminogen Activator in Human Ovarian Cancer Cells.

Author

Walker, John M., Bartlett, John M. S., and Dumler, Inna

Abstract

The ability to fractionate nucleic acids and to determine which of them has sequences complementary to an array of DNA or RNA molecules is one of the most powerful tools of molecular biology. The Southern blot, named for its inventor, is a method for transferring size-fractionated DNA from a gel matrix to a solid support followed by hybridization to a labeled probe (1. The identical process for RNA became known as) the Northern blot i2 1i The identical process for RNA became known as the Northern blot (2). Both are, then, often key elements in establishing the identity of nucleic acids of interest. Northern blot analysis was used by us as a tool in order to answer the question whether or not the nuclear transcripitional apparatus of human ovarian cancer cells might be activated in response to the urokinase. [ABSTRACT FROM AUTHOR]

Published
2001
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