Search

Your search keyword '"Duesund, Henrik"' showing total 17 results
Start Over Author "Duesund, Henrik"
17 results on '"Duesund, Henrik"'

6. Characterization of a VHS virus genotype III isolated from rainbow trout (Oncorhychus mykiss) at a marine site on the west coast of Norway

Author

Ottem Karl F, Watanabe Kuninori, Nylund Stian, Duesund Henrik, and Nylund Are

Subjects
Infectious and parasitic diseases, RC109-216
Abstract

Abstract Background Norwegian production of rainbow trout (Oncorhynchus mykiss) has been without any outbreaks of VHS for many years until the disease emerged in a farm in western Norway in November 2007. The fish were, in addition to VHS virus, positive for gill chlamydia-like bacteria, Flavobacterium psychrophilum, and a microsporidian. A new VHS virus genotype III was isolated from the fish in RTgill-W1 cells and the complete coding region (11,065 nucleotides) was sequenced. This virus was also used in a challenge experiment to see if it could cause any mortality in rainbow trout in sea water. Results This is the first time a nearly complete sequence of a genotype III virus isolate has been presented. The organization of the genes is the same as in the other VHS virus genotypes studied (GI and GIV). Between the ORFs are nontranslated regions that contain highly conserved sequences encompassing the polyadenylation signal for one gene, and the putative transcription initiation site of the next gene. The intergenic regions vary in length from 74 nt to 128 nt. The nucleotide sequence is more similar to genotype I isolates compared to isolates from genotype II and IV. Analyses of the sequences of the N and G protein genes show that this new isolate is distinct from other VHS virus isolates and groups closely together with isolates from genotype III. In a challenge experiment, using intraperitoneal (ip) injection of the isolate, co-habitation with infected fish, and bath challenge, mortalities slightly above 40% were obtained. There was no significant difference in mortality between the bath challenged group and the ip injected group, while the mortality in the co-habitation group was as low as 30%. Conclusions All VHS virus isolates in genotype III are from marine fish in the North East Atlantic. Unlike the other known genotype III isolates, which are of low virulence, this new isolate is moderately virulent. It was not possible to detect any changes in the virus genome that could explain the higher virulence. A major problem for the study of virulence factors is the lack of information about other genotype III isolates.

Published
2010
Full Text
View/download PDF

16. Primary Isolation and Characterization of Tenacibaculum maritimumfrom Chilean Atlantic Salmon Mortalities Associated with a Pseudochattonellaspp. Algal Bloom

Author

Apablaza, Patricia, Frisch, Kathleen, Brevik, Øyvind Jakobsen, Småge, Sverre Bang, Vallestad, Camilla, Duesund, Henrik, Mendoza, Julio, and Nylund, Are

Abstract

This study presents the first isolation of Tenacibaculum maritimumfrom farmed Atlantic Salmon Salmo salarin Chile. The isolate, designated T. maritimumCh-2402, was isolated from gills of Atlantic Salmon at a farm located in region X, Los Lagos, Chile, during the harmful algal bloom caused by Pseudochattonellaspp. in February 2016. The algal bloom is reported to have caused 40,000 metric tons of mortality in this salmon farming area. The bacterium T. maritimum, which causes tenacibaculosis, is recognized as an important pathogen of marine fish worldwide. Genetic, phylogenetic, and phenotypic characterizations were used to describe the T. maritimumCh-2402 isolate. The isolate was similar to the type strain of T. maritimumbut was genetically unique. Tenacibaculum dicentrarchiisolates were also recovered during sampling from the same farm. Based on the fact that T. maritimumhas been shown to cause disease in Atlantic Salmon in other regions, the presence of this bacterium poses a potential risk of disease to fish in the Chilean aquaculture industry. Received November 6, 2016; accepted May 29, 2017 Published online July 26, 2017

Published
2017
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results