7,508 results on '"Dubuc A"'
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2. From Yoneda to Topoi morphisms
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Dubuc, Eduardo J.
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Mathematics - Category Theory ,18F10 - Abstract
In this note we show how two fundamental results in Topos theory follow by repeated use of Yoneda's Lemma, the formalism of natural transformations and very basic category theory. In Lemma 9.4, we show the fundamental result SGA4 EXPOSE IV Proposition 4.9.4, which says that for any site $\cc{C}$, the canonical functor $\cc{C} \mr{\varepsilon} Sh(\cc{C})$ into the category of sheaves, classifies sites morphisms $\cc{C} \mr{} \cc{Z}$ into any topos $\cc{Z}$. After the usual Yoneda's Lemma, Lemma 3.1, we show that the Yoneda functor $\cc{C} \mr{h} \Eop{C}$ classifies functors $\cc{C} \mr{} \cc{Z}$ into any cocomplete category $\cc{Z}$, via a cocontinuos extension $\Eop{C} \mr{} \cc{Z}$, Lemma 5.2. Then we reach Lemma 9.4 by an step by step enrichment of 5.2. All we use is Yoneda's Lemma, over and over again, and the Yoga of natural transformations. In Lemma 10.1 we show the equivalence between \emph{flatness} and \emph{left exactness} for functors from finitely complete categories into any topos. Our proof is elementary, we show how basic exactness properties of sets prove the result for set valued functors, then we generalize to functors valued in any topos utilizing results of the previous sections and the Yoga of natural transformations. Besides the thread we follow,nothing here is new, although we haven't seen 10.1 proved this way before., Comment: 11 pages, a thread from Yoneda's Lemma to SGA4 classification of site morphisms, a proof corrected
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- 2023
3. Validity of luminometry and bacteriological tests for diagnosing intramammary infection at dry-off in dairy cows
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J. Denis-Robichaud, N. Barbeau-Grégoire, M.-L. Gauthier, S. Dufour, J.-P. Roy, S. Buczinski, and J. Dubuc
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Tri-Plate ,Petrifilm ,Bayesian latent class analysis ,predictive values ,misclassification cost term ,Dairy processing. Dairy products ,SF250.5-275 ,Dairying ,SF221-250 - Abstract
ABSTRACT: The objective of this cross-sectional study was to estimate the validity of laboratory culture, Petrifilm and Tri-Plate on-farm culture systems, as well as luminometry to correctly identify IMI at dry-off in dairy cows, considering all tests to be imperfect. From September 2020 until December 2021, we collected composite milk samples from cows before dry-off and divided them into 4 aliquots for luminometry, Petrifilm (aerobic count), Tri-Plate, and laboratory culture tests. We assessed multiple thresholds of relative light units (RLU) for luminometry, and we used thresholds of ≥100 cfu/mL for the laboratory culture, ≥50 cfu/mL for Petrifilm, and ≥1 cfu for Tri-Plate tests. We fitted Bayesian latent class analysis models to estimate the sensitivity (Se) and specificity (Sp) for each test to identify IMI, with 95% credibility interval (BCI). Using different prevalence measures (0.30, 0.50, and 0.70), we calculated the predictive values (PV) and misclassification cost terms (MCT) at different false negative-to-false-positive ratios (FN:FP). A total of 333 cows were enrolled in the study from one commercial Holstein herd. The validity of the luminometry was poor for all thresholds, with an Se of 0.51 (95% BCI = 0.43–0.59) and Sp of 0.38 (95% BCI = 0.26–0.50) when using a threshold of ≥150 RLU. The laboratory culture had an Se of 0.93 (95% BCI = 0.85–0.98) and Sp of 0.69 (95% BCI = 0.49–0.89); the Petrifilm had an Se of 0.91 (95% BCI = 0.80–0.98) and Sp of 0.71 (95% BCI = 0.51–0.90); and the Tri-Plate had an Se of 0.65 (95% BCI = 0.53–0.82) and Sp of 0.85 (95% BCI = 0.66–0.97). Bacteriological tests had good PV, with comparable positive PV for all 3 tests, but lower negative PV for the Tri-Plate compared with the laboratory culture and the Petrifilm. For a prevalence of IMI of 0.30, all 3 tests had similar MCT, but for prevalence of 0.50 and 0.70, the Tri-Plate had higher MCT in scenarios where leaving a cow with IMI untreated is considered to have greater detrimental effects than treating a healthy cow (i.e., FN:FP of 3:1). Our results showed that the bacteriological tests have adequate validity to diagnose IMI at dry-off, but luminometry does not. We concluded that although luminometry is not useful to identify IMI at dry-off, the Petrifilm and Tri-Plate tests performed similarly to laboratory culture, depending on the prevalence and importance of the FP and FN results.
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- 2024
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4. Mental health service use and shortages among a cohort of women living with HIV in Canada
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Seerat Chawla, Angela Kaida, Marie-Josée Brouillette, Bluma Kleiner, Danièle Dubuc, Lashanda Skerritt, Ann N. Burchell, Danielle Rouleau, Mona Loutfy, Alexandra de Pokomandy, and the CHIWOS research team
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Mental health care ,Mental health needs ,Service use ,Women living with HIV ,Public aspects of medicine ,RA1-1270 - Abstract
Abstract Background The prevalence of mental health conditions among women with HIV in Canada ranges between 29.5% and 57.4%, highlighting the need for accessible mental health care. We aimed to (1) describe the availability and use of mental health services among women with HIV and (2) identify characteristics associated with reporting that shortages of these services presented a problem in their care. Methods Baseline data from the Canadian HIV Women’s Sexual and Reproductive Health Cohort Study were analysed. Self-reported availability and use of mental health services were examined using descriptive statistics. Participants indicated whether a lack of mental health support was a problem in their care. Logistic regression models were constructed to determine associations between sociodemographic, clinical, and psychosocial characteristics and reported problematic shortages. Results Of 1422 women, 26.7% (n = 380) used mental health services in the last year, which most accessed through their HIV clinic. Thirty-eight percent (n = 541) reported that a shortage of mental health support was a problem in their care. Among this subset, 22.1% (n = 119) used services at their HIV clinic, 26.5% (n = 143) reported available services but did not use them, and 51.4% (n = 277) either indicated that these services were unavailable, did not know if such services were available, or were unengaged in HIV care. Factors associated with reporting problematic shortages included rural residence [adjusted odds ratio (aOR): 1.69, 95% confidence interval (CI): 1.03–2.77], higher education level (aOR: 1.43, 95% CI: 1.02–2.02), and higher HIV stigma score (aOR: 1.03, 95% CI: 1.02–1.03). Conversely, African/Caribbean/Black identity (aOR: 0.37, 95% CI: 0.26–0.54), history of recreational drug use (aOR: 0.56, 95% CI: 0.39–0.81), and Quebec residence (aOR: 0.69, 95% CI: 0.50–0.96) were associated with lower odds of reporting service shortages. Conclusion Our findings highlight the HIV clinic as the primary location of mental health service use. However, existing services may not be sufficient to reach all patients or meet specific needs. Furthermore, the low uptake among those reporting a shortage suggests a lack of connection to services or patient knowledge about their availability. Characteristics associated with reporting shortages reflect geographic and socioeconomic disparities that must be accounted for in future service design.
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- 2024
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5. TrackIops: Real-Time NFS Performance Metrics Extractor.
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Théophile Dubuc, Pascale Vicat-Blanc, Pierre Olivier, Mar Callau-Zori, Christophe Hubert, and Alain Tchana
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- 2024
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6. Mental health service use and shortages among a cohort of women living with HIV in Canada
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Chawla, Seerat, Kaida, Angela, Brouillette, Marie-Josée, Kleiner, Bluma, Dubuc, Danièle, Skerritt, Lashanda, Burchell, Ann N., Rouleau, Danielle, Loutfy, Mona, and de Pokomandy, Alexandra
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- 2024
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7. SARS-CoV-2 rapidly evolves lineage-specific phenotypic differences when passaged repeatedly in immune-naïve mice
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Willett, Julian Daniel Sunday, Gravel, Annie, Dubuc, Isabelle, Gudimard, Leslie, dos Santos Pereira Andrade, Ana Claudia, Lacasse, Émile, Fortin, Paul, Liu, Ju-Ling, Cervantes, Jose Avila, Galvez, Jose Hector, Djambazian, Haig Hugo Vrej, Zwaig, Melissa, Roy, Anne-Marie, Lee, Sally, Chen, Shu-Huang, Ragoussis, Jiannis, and Flamand, Louis
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- 2024
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8. Facial and genital color ornamentation, testosterone, and reproductive output in high-ranking male rhesus macaques
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Sobral, Gisela, Dubuc, Constance, Winters, Sandra, Ruiz‑Lambides, Angelina, Emery Thompson, Melissa, Maestripieri, Dario, and Milich, Krista M.
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- 2024
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9. Outdoor Characterization of Solar Cells with Micro-structured Anti-Reflective Coating in a Concentrator Photovoltaic Module
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Leoga, Arnaud J. K., Ritou, Arnaud, Blanchard, Mathieu, Dirand, Lysandre, Prunier, Yanis, St-Pierre, Philippe, Chuet, David, Provost, Philippe-Olivier, Volatier, Maite, Aimez, Vincent, Hamon, Gwenaelle, Jaouad, Abdelatif, Dubuc, Christian, and Darnon, Maxime
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Electrical Engineering and Systems Science - Systems and Control - Abstract
Micro-structured anti reflective coatings (ARC) have been identified as a promising solution to reduce optical losses in Concentrator Photovoltaics modules (CPV). We fabricated and tested in field a CPV modules made of 4 sub-modules with a concentration factor of 250x, that embed either solar cells with micro-structured encapsulating ARC or solar cells with multilayer ARC as a reference. The micro-structured encapsulating ARC was made of semi-buried silica beads in polydimethylsiloxane (PDMS). The module was in operation for 1 year in the severe climatic conditions of Sherbrooke, Quebec, Canada, before extracting the sub-modules performance under Concentrator Standard Operating Condition (CSOC). An acceptance angle of +/-0.78 degree was determined for all sub-modules, demonstrating that improving angular collection at the cell level has no significant impact on the angle of acceptance at the module level. We report an increase of 12 to 14% of the short-circuit current and of 15 to 19% of maximum power at CSOC for solar cells with a micro structured encapsulating ARC compared to the reference. Despite a sub-optimal module design, we report a sub-module efficiency of 29.7% at CSOC for a cell with micro-structured encapsulating ARC. This proves the potential of micro-structured encapsulating ARC to improve CPV system performance and shows promise of reliability for sumi-buried microbeads in PDMS as encapsulating ARC., Comment: 6 pages 6 figures 3 tables
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- 2023
10. Herd-level associations between the proportion of elevated prepartum nonesterified fatty acid concentrations and postpartum diseases, reproduction, or culling on dairy farms
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J. Denis-Robichaud, I. Nicola, H. Chupin, J.-P. Roy, S. Buczinski, V. Fauteux, N. Picard-Hagen, R. Cue, and J. Dubuc
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Dairy processing. Dairy products ,SF250.5-275 - Abstract
The objectives of this herd-level prospective observational cohort study were to describe the proportion of cows with elevated prepartum nonesterified fatty acid concentrations (PropElevNEFA) in dairy herds and to assess the herd-level associations between PropElevNEFA and postpartum diseases, reproductive performance, and culling. From November 2018 to December 2020, a convenience sample of 49 herds was enrolled in this study. Blood sampling (16 to 29 cows per herd) was performed during the week before and during the 2 wk following calving to quantify the concentration of nonesterified fatty acids (NEFA) and β-hydroxybutyrate acids (BHBA), respectively. Elevated NEFA was defined as ≥280 µmol/L and hyperketonemia as BHBA ≥1.4 mmol/L. Retained placenta, metritis, purulent vaginal discharge, endometritis, and mastitis were diagnosed on-farm following standardized definitions, and success at first artificial insemination (AI) and culling events were recorded. The associations between PropElevNEFA and each individual disease, success at first AI, and culling were evaluated using Bayesian aggregated binomial regression models with weakly informative priors, from the which odds ratio (OR) and the 95% credible intervals (BCI) were obtained. A total of 981 cows were included in the statistical analyses representing 16 to 29 (median = 19) cows per herd. Cows were enrolled in the prepartum period of their first to tenth (median = third) lactation, and 41% of them had an elevated prepartum NEFA concentration. At the herd level, PropElevNEFA varied between 11% and 78% (median = 39%). The odds of metritis (OR = 1.37, 95% BCI = 1.13–1.67) increased for every 10-point increase in PropElevNEFA, whereas the odds of success at first AI decreased (OR = 0.69, 95% BCI = 0.59–0.80). The PropElevNEFA was not associated with the other tested diseases or culling. Our results suggest that the herd-level proportion of cows having elevated prepartum NEFA concentrations is associated with metritis and poor success at first AI in dairy herds.
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- 2024
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11. Wafer-scale detachable monocrystalline Germanium nanomembranes for the growth of III-V materials and substrate reuse
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Paupy, Nicolas, Elhmaidi, Zakaria Oulad, Chapotot, Alexandre, Hanuš, Tadeáš, Arias-Zapata, Javier, Ilahi, Bouraoui, Heintz, Alexandre, Mbeunmi, Alex Brice Poungoué, Arvinte, Roxana, Aziziyan, Mohammad Reza, Daniel, Valentin, Hamon, Gwenaëlle, Chrétien, Jérémie, Zouaghi, Firas, Ayari, Ahmed, Mouchel, Laurie, Henriques, Jonathan, Demoulin, Loïc, Diallo, Thierno Mamoudou, Provost, Philippe-Olivier, Pelletier, Hubert, Volatier, Maïté, Kurstjens, Rufi, Cho, Jinyoun, Courtois, Guillaume, Dessein, Kristof, Arcand, Sébastien, Dubuc, Christian, Jaouad, Abdelatif, Quaegebeur, Nicolas, Gosselin, Ryan, Machon, Denis, Arès, Richard, Darnon, Maxime, and Boucherif, Abderraouf
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Condensed Matter - Materials Science ,Physics - Applied Physics - Abstract
Germanium (Ge) is increasingly used as a substrate for high-performance optoelectronic, photovoltaic, and electronic devices. These devices are usually grown on thick and rigid Ge substrates manufactured by classical wafering techniques. Nanomembranes (NMs) provide an alternative to this approach while offering wafer-scale lateral dimensions, weight reduction, limitation of waste, and cost effectiveness. Herein, we introduce the Porous germanium Efficient Epitaxial LayEr Release (PEELER) process, which consists of the fabrication of wafer-scale detachable monocrystalline Ge NMs on porous Ge (PGe) and substrate reuse. We demonstrate monocrystalline Ge NMs with surface roughness below 1 nm on top of nanoengineered void layer enabling layer detachment. Furthermore, these Ge NMs exhibit compatibility with the growth of III-V materials. High-resolution transmission electron microscopy (HRTEM) characterization shows Ge NMs crystallinity and high-resolution X-ray diffraction (HRXRD) reciprocal space mapping endorses high-quality GaAs layers. Finally, we demonstrate the chemical reconditioning process of the Ge substrate, allowing its reuse, to produce multiple free-standing NMs from a single parent wafer. The PEELER process significantly reduces the consumption of Ge during the fabrication process which paves the way for a new generation of low-cost flexible optoelectronics devices., Comment: 17 pages and 6 figures along with 3 figures in supporting information
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- 2022
12. Efficacy of COMPAs, an App Designed to Support Communication Between Persons Living With Dementia in Long-Term Care Settings and Their Caregivers: Mixed Methods Implementation Study
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Ana Inés Ansaldo, Michèle Masson-Trottier, Barbara Delacourt, Jade Dubuc, and Catherine Dubé
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Geriatrics ,RC952-954.6 - Abstract
BackgroundPersons living with dementia experience autonomy loss and require caregiver support on a daily basis. Dementia involves a gradual decline in communication skills, leading to fewer interactions and isolation for both people living with dementia and their caregivers, negatively impacting the quality of life for both members of the dyad. The resulting stress and burden on caregivers make them particularly susceptible to burnout. ObjectiveThis study aims to examine the efficacy of Communication Proches Aidants (COMPAs), an app designed following the principles of person-centered and emotional communication, which is intended to improve well-being in persons living with dementia and caregivers and reduce caregiver burden. MethodsIn this implementation study, volunteer caregivers in 2 long-term care facilities (n=17) were trained in using COMPAs and strategies to improve communication with persons living with dementia. Qualitative and quantitative analyses, semistructured interviews, and questionnaires were completed before and after 8 weeks of intervention with COMPAs. ResultsSemistructured interviews revealed that all caregivers perceived a positive impact following COMPAs interventions, namely, improved quality of communication and quality of life among persons living with dementia and caregivers. Improved quality of life was also supported by a statistically significant reduction in the General Health Questionnaire-12 scores (caregivers who improved: 9/17, 53%; z=2.537; P=.01). COMPAs interventions were also associated with a statistically significant increased feeling of personal accomplishment (caregivers improved: 11/17, 65%; t15=2.430; P=.03; d=0.61 [medium effect size]). ConclusionsCOMPAs intervention improved well-being in persons living with dementia and their caregivers by developing person-centered communication within the dyad, increasing empathy, and reducing burden in caregivers although most caregivers were unfamiliar with technology. The results hold promise for COMPAs interventions in long-term care settings. Larger group-controlled studies with different populations, in different contexts, and at different stages of dementia will provide a clearer picture of the benefits of COMPAs interventions.
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- 2024
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13. The neurophysiological brain-fingerprint of Parkinson’s diseaseResearch in context
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Jason da Silva Castanheira, Alex I. Wiesman, Justine Y. Hansen, Bratislav Misic, Sylvain Baillet, John Breitner, Judes Poirier, Pierre Bellec, Véronique Bohbot, Mallar Chakravarty, Louis Collins, Pierre Etienne, Alan Evans, Serge Gauthier, Rick Hoge, Yasser Ituria-Medina, Gerhard Multhaup, Lisa-Marie Münter, Natasha Rajah, Pedro Rosa-Neto, Jean-Paul Soucy, Etienne Vachon-Presseau, Sylvia Villeneuve, Philippe Amouyel, Melissa Appleby, Nicholas Ashton, Daniel Auld, Gülebru Ayranci, Christophe Bedetti, Marie-Lise Beland, Kaj Blennow, Ann Brinkmalm Westman, Claudio Cuello, Mahsa Dadar, Leslie-Ann Daoust, Samir Das, Marina Dauar-Tedeschi, Louis De Beaumont, Doris Dea, Maxime Descoteaux, Marianne Dufour, Sarah Farzin, Fabiola Ferdinand, Vladimir Fonov, Julie Gonneaud, Justin Kat, Christina Kazazian, Anne Labonté, Marie-Elyse Lafaille-Magnan, Marc Lalancette, Jean-Charles Lambert, Jeannie-Marie Leoutsakos, Laura Mahar, Axel Mathieu, Melissa McSweeney, Pierre-François Meyer, Justin Miron, Jamie Near, Holly NewboldFox, Nathalie Nilsson, Pierre Orban, Cynthia Picard, Alexa Pichet Binette, Jean-Baptiste Poline, Sheida Rabipour, Alyssa Salaciak, Matthew Settimi, Sivaniya Subramaniapillai, Angela Tam, Christine Tardif, Louise Théroux, Jennifer Tremblay-Mercier, Stephanie Tullo, Irem Ulku, Isabelle Vallée, Henrik Zetterberg, Vasavan Nair, Jens Pruessner, Paul Aisen, Elena Anthal, Alan Barkun, Thomas Beaudry, Fatiha Benbouhoud, Jason Brandt, Leopoldina Carmo, Charles Edouard Carrier, Laksanun Cheewakriengkrai, Blandine Courcot, Doris Couture, Suzanne Craft, Christian Dansereau, Clément Debacker, René Desautels, Sylvie Dubuc, Guerda Duclair, Mark Eisenberg, Rana El-Khoury, Anne-Marie Faubert, David Fontaine, Josée Frappier, Joanne Frenette, Guylaine Gagné, Valérie Gervais, Renuka Giles, Renee Gordon, Clifford Jack, Benoit Jutras, Zaven Khachaturian, David Knopman, Penelope Kostopoulos, Félix Lapalme, Tanya Lee, Claude Lepage, Illana Leppert, Cécile Madjar, David Maillet, Jean-Robert Maltais, Sulantha Mathotaarachchi, Ginette Mayrand, Diane Michaud, Thomas Montine, John Morris, Véronique Pagé, Tharick Pascoal, Sandra Peillieux, Mirela Petkova, Galina Pogossova, Pierre Rioux, Mark Sager, Eunice Farah Saint-Fort, Mélissa Savard, Reisa Sperling, Shirin Tabrizi, Pierre Tariot, Eduard Teigner, Ronald Thomas, Paule-Joanne Toussaint, Miranda Tuwaig, Vinod Venugopalan, Sander Verfaillie, Jacob Vogel, Karen Wan, Seqian Wang, Elsa Yu, Isabelle Beaulieu-Boire, Pierre Blanchet, Sarah Bogard, Manon Bouchard, Sylvain Chouinard, Francesca Cicchetti, Martin Cloutier, Alain Dagher, Clotilde Degroot, Alex Desautels, Marie Hélène Dion, Janelle Drouin-Ouellet, Anne-Marie Dufresne, Nicolas Dupré, Antoine Duquette, Thomas Durcan, Lesley K. Fellows, Edward Fon, Jean-François Gagnon, Ziv Gan-Or, Angela Genge, Nicolas Jodoin, Jason Karamchandani, Anne-Louise Lafontaine, Mélanie Langlois, Etienne Leveille, Martin Lévesque, Calvin Melmed, Oury Monchi, Jacques Montplaisir, Michel Panisset, Martin Parent, Minh-Thy Pham-An, Ronald Postuma, Emmanuelle Pourcher, Trisha Rao, Jean Rivest, Guy Rouleau, Madeleine Sharp, Valérie Soland, Michael Sidel, Sonia Lai Wing Sun, Alexander Thiel, and Paolo Vitali
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Movement disorders ,Parkinson’s disease ,Neural dynamics ,Oscillations ,Arrhythmic brain activity ,Magnetoencephalography ,Medicine ,Medicine (General) ,R5-920 - Abstract
Summary: Background: Research in healthy young adults shows that characteristic patterns of brain activity define individual “brain-fingerprints” that are unique to each person. However, variability in these brain-fingerprints increases in individuals with neurological conditions, challenging the clinical relevance and potential impact of the approach. Our study shows that brain-fingerprints derived from neurophysiological brain activity are associated with pathophysiological and clinical traits of individual patients with Parkinson’s disease (PD). Methods: We created brain-fingerprints from task-free brain activity recorded through magnetoencephalography in 79 PD patients and compared them with those from two independent samples of age-matched healthy controls (N = 424 total). We decomposed brain activity into arrhythmic and rhythmic components, defining distinct brain-fingerprints for each type from recording durations of up to 4 min and as short as 30 s. Findings: The arrhythmic spectral components of cortical activity in patients with Parkinson’s disease are more variable over short periods, challenging the definition of a reliable brain-fingerprint. However, by isolating the rhythmic components of cortical activity, we derived brain-fingerprints that distinguished between patients and healthy controls with about 90% accuracy. The most prominent cortical features of the resulting Parkinson’s brain-fingerprint are mapped to polyrhythmic activity in unimodal sensorimotor regions. Leveraging these features, we also demonstrate that Parkinson’s symptom laterality can be decoded directly from cortical neurophysiological activity. Furthermore, our study reveals that the cortical topography of the Parkinson’s brain-fingerprint aligns with that of neurotransmitter systems affected by the disease’s pathophysiology. Interpretation: The increased moment-to-moment variability of arrhythmic brain-fingerprints challenges patient differentiation and explains previously published results. We outline patient-specific rhythmic brain signaling features that provide insights into both the neurophysiological signature and symptom laterality of Parkinson’s disease. Thus, the proposed definition of a rhythmic brain-fingerprint of Parkinson’s disease may contribute to novel, refined approaches to patient stratification. Symmetrically, we discuss how rhythmic brain-fingerprints may contribute to the improved identification and testing of therapeutic neurostimulation targets. Funding: Data collection and sharing for this project was provided by the Quebec Parkinson Network (QPN), the Pre-symptomatic Evaluation of Novel or Experimental Treatments for Alzheimer’s Disease (PREVENT-AD; release 6.0) program, the Cambridge Centre for Aging Neuroscience (Cam-CAN), and the Open MEG Archives (OMEGA). The QPN is funded by a grant from Fonds de Recherche du Québec - Santé (FRQS). PREVENT-AD was launched in 2011 as a $13.5 million, 7-year public-private partnership using funds provided by McGill University, the FRQS, an unrestricted research grant from Pfizer Canada, the Levesque Foundation, the Douglas Hospital Research Centre and Foundation, the Government of Canada, and the Canada Fund for Innovation. The Brainstorm project is supported by funding to SB from the NIH (R01-EB026299-05). Further funding to SB for this study included a Discovery grant from the Natural Sciences and Engineering Research Council of Canada of Canada (436355-13), and the CIHR Canada research Chair in Neural Dynamics of Brain Systems (CRC-2017-00311).
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- 2024
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14. SVD: A Scalable Virtual Machine Disk Format.
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Kevin Nguetchouang, Stella Bitchebe, Théophile Dubuc, Mar Callau-Zori, Christophe Hubert, Pierre Olivier, and Alain Tchana
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- 2024
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15. The 2-localization of a model category
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Dubuc, Eduardo J. and Girabel, Jaqueline
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Mathematics - Category Theory - Abstract
In this paper we study a 2-dimensional version of Quillen's homotopy category construction. Given a category $\mathscr{A}$ and a class of morphisms $\Sigma \subset \mathscr{A}$ containing the identities, we construct a 2-category $\mathcal{H}o(\mathscr{A})$ obtained by the addition of 2-cells determined by homotopies. A salient feature here is the use of a novel notion of cylinder introduced in \cite{e.d.2}. The inclusion 2-functor $\mathscr{C} \longrightarrow \mathcal{H}o(\mathscr{A})$ has a universal property which implies that it will be the 2-localization of $\mathscr{A}$ at $\Sigma$ as soon as the arrows of $\Sigma$ become equivalences in $\mathcal{H}o(\mathscr{A})$. This is then used to obtain 2-localizations of a model category $\mathscr{A}{C}$, with $\Sigma = \mathcal{W}$, the weak equivalences, and $\mathscr{A} = \mathscr{C}_{fc}$, the full subcategory of fibrant-cofibrant objects, as well as with $\mathscr{A} = \mathscr{C}$. The set of connected components of the hom categories yields Quillen's results. We follow the general lines established in \cite{e.d.2}, \cite{e.d.} for model bicategories. The development here is not just the examination of the general theory in a particular case. It is not concerned with and avoids the problems which arise when dealing with non invertible 2-cells. Also, the use here of functorial factorization adds further simplifications by eliminating the need of pseudofunctors. New proofs are produced which are not a mere simplified adaptation of the ones of the general case., Comment: 48 pages, 1 figure, many diagrams
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- 2022
16. The mesencephalic locomotor region recruits V2a reticulospinal neurons to drive forward locomotion in larval zebrafish
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Carbo-Tano, Martin, Lapoix, Mathilde, Jia, Xinyu, Thouvenin, Olivier, Pascucci, Marco, Auclair, François, Quan, Feng B., Albadri, Shahad, Aguda, Vernie, Farouj, Younes, Hillman, Elizabeth M. C., Portugues, Ruben, Del Bene, Filippo, Thiele, Tod R., Dubuc, Réjean, and Wyart, Claire
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- 2023
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17. Correction : Les schémas de subdivision de Besicovitch et de Cantor
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Dubuc, Serge
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- 2023
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18. SARS-CoV-2 rapidly evolves lineage-specific phenotypic differences when passaged repeatedly in immune-naïve mice
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Julian Daniel Sunday Willett, Annie Gravel, Isabelle Dubuc, Leslie Gudimard, Ana Claudia dos Santos Pereira Andrade, Émile Lacasse, Paul Fortin, Ju-Ling Liu, Jose Avila Cervantes, Jose Hector Galvez, Haig Hugo Vrej Djambazian, Melissa Zwaig, Anne-Marie Roy, Sally Lee, Shu-Huang Chen, Jiannis Ragoussis, and Louis Flamand
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Biology (General) ,QH301-705.5 - Abstract
Abstract The persistence of SARS-CoV-2 despite the development of vaccines and a degree of herd immunity is partly due to viral evolution reducing vaccine and treatment efficacy. Serial infections of wild-type (WT) SARS-CoV-2 in Balb/c mice yield mouse-adapted strains with greater infectivity and mortality. We investigate if passaging unmodified B.1.351 (Beta) and B.1.617.2 (Delta) 20 times in K18-ACE2 mice, expressing the human ACE2 receptor, in a BSL-3 laboratory without selective pressures, drives human health-relevant evolution and if evolution is lineage-dependent. Late-passage virus causes more severe disease, at organism and lung tissue scales, with late-passage Delta demonstrating antibody resistance and interferon suppression. This resistance co-occurs with a de novo spike S371F mutation, linked with both traits. S371F, an Omicron-characteristic mutation, is co-inherited at times with spike E1182G per Nanopore sequencing, existing in different within-sample viral variants at others. Both S371F and E1182G are linked to mammalian GOLGA7 and ZDHHC5 interactions, which mediate viral-cell entry and antiviral response. This study demonstrates SARS-CoV-2’s tendency to evolve with phenotypic consequences, its evolution varying by lineage, and suggests non-dominant quasi-species contribution.
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- 2024
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19. Facial and genital color ornamentation, testosterone, and reproductive output in high-ranking male rhesus macaques
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Gisela Sobral, Constance Dubuc, Sandra Winters, Angelina Ruiz‑Lambides, Melissa Emery Thompson, Dario Maestripieri, and Krista M. Milich
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Medicine ,Science - Abstract
Abstract Males in many vertebrate species have colorful ornaments that evolved by sexual selection. The role of androgens in the genesis and maintenance of these signals is unclear. We studied 21 adult high-ranking male rhesus macaques from nine social groups in the free-ranging population on Cayo Santiago, Puerto Rico, and analyzed facial and genital skin luminance and redness, fecal androgens, rates of mating behaviors, and offspring sired. Facial and genital coloration varied in relation to age, mating behavior, reproductive success, and testosterone concentration. Our results indicate that skin coloration in high-ranking male rhesus macaques is a sexually-selected trait mediated by androgens. These results add to the growing literature on the proximate and ultimate causes of male sexual signals and highlight the need to examine how these characteristics change with age in other species.
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- 2024
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20. Effects of soaked hay on lung function and airway inflammation in horses with severe asthma
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Roxane Westerfeld, Flavie Payette, Valérie Dubuc, Estelle Manguin, Khristine Picotte, Guy Beauchamp, Christian Bédard, and Mathilde Leclere
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equine ,heaves ,inflammatory airway disease ,recurrent airway obstruction ,Veterinary medicine ,SF600-1100 - Abstract
Abstract Background Reducing inhaled dust particles improves lung function in horses with severe asthma. Soaked hay is commonly used by owners, but its efficacy in improving lung function and inflammation has not been documented. Objectives To measure the effects of soaked hay and alfalfa pellets in horses with severe asthma. Animals Ten adult horses with severe asthma from a research colony. Methods Prospective controlled trial. Horses in clinical exacerbation were housed indoors and allocated to be fed either soaked hay (n = 5) or alfalfa pellets (n = 5) for 6 weeks. Soaked hay was immersed for 45 minutes and dried out hay was discarded between meals. Pulmonary function and clinical scores were measured before and after 2, 4, and 6 weeks. Tracheal mucus scores and bronchoalveolar lavages were performed before and after 6 weeks. Lung function was analyzed with a linear mixed model using log‐transformed data. Results Lung resistance decreased from (median (range)) 2.47 (1.54‐3.95) to 1.59 (0.52‐2.10) cmH2O/L/s in the pellets group and from 1.89 (1.2‐3.54) to 0.61 (0.42‐2.08) cmH2O/L/s in the soaked hay group over the 6‐week period for an average difference of 1.06 cmH2O/L/s for pellets (95% confidence interval [95% CI]: 0.09‐2.04, P = .03, not significant after correction) and 1.31 cmH2O/L/s for soaked hay (95% CI: −0.23 to 2.85, P
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- 2024
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21. Virtual Disk Snapshot Management at Scale
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Nguetchouang, Kevin, Dubuc, Theophile, Bitchebe, Stella, Tchana, Alain, and Olivier, Pierre
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Computer Science - Distributed, Parallel, and Cluster Computing - Abstract
Contrary to the other resources such as CPU, memory, and network, for which virtualization is efficiently achieved through direct access, disk virtualization is peculiar. In this paper, we make four contributions. Our first contribution is the characterization of disk utilization in a public large-scale cloud infrastructure. It reveals the presence of long snapshot chains, sometimes composed of up to 1000 files. Our second contribution is to show that long chains lead to performance and memory footprint scalability issues by experimental measurements. Our third contribution is the extension of the Qcow2 format and its driver in Qemu to address the identified scalability challenges. Our fourth contribution is the thorough evaluation of our prototype, called sQemu, demonstrating that it brings significant performance enhancements and memory footprint reduction. For example, it improves the throughput of RocksDB by about 48% compared to vanilla Qemu on a snapshot chain of length 500. The memory overhead on that chain is also reduced by 15x.
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- 2022
22. Multimodal sexual signals are not precise indicators of fertility in female Kinda baboons
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Petersdorf, Megan, Weyher, Anna H., Heistermann, Michael, Gunson, Jessica L., Govaerts, Alison, Siame, Simon, Mustill, Ruby L., Hillegas, Madison E., Winters, Sandra, Dubuc, Constance, and Higham, James P.
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- 2024
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23. Validity of luminometry and bacteriological tests for diagnosing intramammary infection at dry-off in dairy cows
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Denis-Robichaud, J., Barbeau-Grégoire, N., Gauthier, M.-L., Dufour, S., Roy, J.-P., Buczinski, S., and Dubuc, J.
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- 2024
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24. Effect of recombinant bovine granulocyte colony-stimulating factor treatment during the peripartum period on postpartum diseases, reproductive performance, and milk production in Holstein cattle
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Yáñez, Uxía, Álvarez, Jacobo, Lorenzo, Guillermo, Caínzos, Juan, Dubuc, Jocelyn, Becerra, Juan J., Herradón, Pedro G., Peña, Ana I., and Quintela, Luis A.
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- 2024
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25. Consequences for nekton of the nature, dynamics, and ecological functioning of tropical tidally dominated ecosystems
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Sheaves, M., Baker, R., Abrantes, K., Barnett, A., Bradley, M., Dubuc, A., Mattone, C., Sheaves, J., and Waltham, N.
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- 2024
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26. Coping with environmental degradation: Physiological and morphological adjustments of wild mangrove fish to decades of aquaculture-induced nutrient enrichment
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Dubuc, A., Rummer, J.L., Vigliola, L., and Lemonnier, H.
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- 2024
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27. A phase 3 randomized trial of mavorixafor, a CXCR4 antagonist, for WHIM syndrome
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Badolato, Raffaele, Alsina, Laia, Azar, Antoine, Bertrand, Yves, Bolyard, Audrey A., Dale, David, Deyà-Martínez, Àngela, Dickerson, Kathryn E., Ezra, Navid, Hasle, Henrik, Kang, Hyoung Jin, Kiani-Alikhan, Sorena, Kuijpers, Taco W., Kulagin, Alexander, Langguth, Daman, Levin, Carina, Neth, Olaf, Olbrich, Peter, Peake, Jane, Rodina, Yulia, Rutten, Caroline E., Shcherbina, Anna, Tarrant, Teresa K., Vossen, Matthias G., Wysocki, Christian A., Belschner, Andrea, Bridger, Gary J., Chen, Kelly, Dubuc, Susan, Hu, Yanping, Jiang, Honghua, Li, Sunny, MacLeod, Rick, Stewart, Murray, Taveras, Arthur G., Yan, Tina, and Donadieu, Jean
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- 2024
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28. The neurophysiological brain-fingerprint of Parkinson’s disease
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Breitner, John, Poirier, Judes, Baillet, Sylvain, Bellec, Pierre, Bohbot, Véronique, Chakravarty, Mallar, Collins, Louis, Etienne, Pierre, Evans, Alan, Gauthier, Serge, Hoge, Rick, Ituria-Medina, Yasser, Multhaup, Gerhard, Münter, Lisa-Marie, Rajah, Natasha, Rosa-Neto, Pedro, Soucy, Jean-Paul, Vachon-Presseau, Etienne, Villeneuve, Sylvia, Amouyel, Philippe, Appleby, Melissa, Ashton, Nicholas, Auld, Daniel, Ayranci, Gülebru, Bedetti, Christophe, Beland, Marie-Lise, Blennow, Kaj, Westman, Ann Brinkmalm, Cuello, Claudio, Dadar, Mahsa, Daoust, Leslie-Ann, Das, Samir, Dauar-Tedeschi, Marina, De Beaumont, Louis, Dea, Doris, Descoteaux, Maxime, Dufour, Marianne, Farzin, Sarah, Ferdinand, Fabiola, Fonov, Vladimir, Gonneaud, Julie, Kat, Justin, Kazazian, Christina, Labonté, Anne, Lafaille-Magnan, Marie-Elyse, Lalancette, Marc, Lambert, Jean-Charles, Leoutsakos, Jeannie-Marie, Mahar, Laura, Mathieu, Axel, McSweeney, Melissa, Meyer, Pierre-François, Miron, Justin, Near, Jamie, NewboldFox, Holly, Nilsson, Nathalie, Orban, Pierre, Picard, Cynthia, Binette, Alexa Pichet, Poline, Jean-Baptiste, Rabipour, Sheida, Salaciak, Alyssa, Settimi, Matthew, Subramaniapillai, Sivaniya, Tam, Angela, Tardif, Christine, Théroux, Louise, Tremblay-Mercier, Jennifer, Tullo, Stephanie, Ulku, Irem, Vallée, Isabelle, Zetterberg, Henrik, Nair, Vasavan, Pruessner, Jens, Aisen, Paul, Anthal, Elena, Barkun, Alan, Beaudry, Thomas, Benbouhoud, Fatiha, Brandt, Jason, Carmo, Leopoldina, Carrier, Charles Edouard, Cheewakriengkrai, Laksanun, Courcot, Blandine, Couture, Doris, Craft, Suzanne, Dansereau, Christian, Debacker, Clément, Desautels, René, Dubuc, Sylvie, Duclair, Guerda, Eisenberg, Mark, El-Khoury, Rana, Faubert, Anne-Marie, Fontaine, David, Frappier, Josée, Frenette, Joanne, Gagné, Guylaine, Gervais, Valérie, Giles, Renuka, Gordon, Renee, Jack, Clifford, Jutras, Benoit, Khachaturian, Zaven, Knopman, David, Kostopoulos, Penelope, Lapalme, Félix, Lee, Tanya, Lepage, Claude, Leppert, Illana, Madjar, Cécile, Maillet, David, Maltais, Jean-Robert, Mathotaarachchi, Sulantha, Mayrand, Ginette, Michaud, Diane, Montine, Thomas, Morris, John, Pagé, Véronique, Pascoal, Tharick, Peillieux, Sandra, Petkova, Mirela, Pogossova, Galina, Rioux, Pierre, Sager, Mark, Saint-Fort, Eunice Farah, Savard, Mélissa, Sperling, Reisa, Tabrizi, Shirin, Tariot, Pierre, Teigner, Eduard, Thomas, Ronald, Toussaint, Paule-Joanne, Tuwaig, Miranda, Venugopalan, Vinod, Verfaillie, Sander, Vogel, Jacob, Wan, Karen, Wang, Seqian, Yu, Elsa, Beaulieu-Boire, Isabelle, Blanchet, Pierre, Bogard, Sarah, Bouchard, Manon, Chouinard, Sylvain, Cicchetti, Francesca, Cloutier, Martin, Dagher, Alain, Degroot, Clotilde, Desautels, Alex, Dion, Marie Hélène, Drouin-Ouellet, Janelle, Dufresne, Anne-Marie, Dupré, Nicolas, Duquette, Antoine, Durcan, Thomas, Fellows, Lesley K., Fon, Edward, Gagnon, Jean-François, Gan-Or, Ziv, Genge, Angela, Jodoin, Nicolas, Karamchandani, Jason, Lafontaine, Anne-Louise, Langlois, Mélanie, Leveille, Etienne, Lévesque, Martin, Melmed, Calvin, Monchi, Oury, Montplaisir, Jacques, Panisset, Michel, Parent, Martin, Pham-An, Minh-Thy, Postuma, Ronald, Pourcher, Emmanuelle, Rao, Trisha, Rivest, Jean, Rouleau, Guy, Sharp, Madeleine, Soland, Valérie, Sidel, Michael, Wing Sun, Sonia Lai, Thiel, Alexander, Vitali, Paolo, da Silva Castanheira, Jason, Wiesman, Alex I., Hansen, Justine Y., and Misic, Bratislav
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- 2024
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29. AI algorithm combined with RNA editing-based blood biomarkers to discriminate bipolar from major depressive disorders in an external validation multicentric cohort
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Salvetat, Nicolas, Checa-Robles, Francisco Jesus, Delacrétaz, Aurélie, Cayzac, Christopher, Dubuc, Benjamin, Vetter, Diana, Dainat, Jacques, Lang, Jean-Philippe, Gamma, Franziska, and Weissmann, Dinah
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- 2024
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30. Health indicators in surplus calves at the time of arrival at auction markets: Associations with distance from farms of origin in Québec, Canada
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Jean Silva Ramos, Marianne Villettaz-Robichaud, Julie Arsenault, Younès Chorfi, Marcio Costa, Jocelyn Dubuc, Salvatore Ferraro, David Francoz, Marjolaine Rousseau, Gilles Fecteau, and Sébastien Buczinski
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transport ,veal calves ,calf health ,animal welfare ,dehydration ,Dairy processing. Dairy products ,SF250.5-275 ,Dairying ,SF221-250 - Abstract
ABSTRACT: This study evaluated the associations between estimated distance from farms' locations to auction markets, and health indicators of surplus dairy calves sold during summer 2019 and winter 2020 in Québec, Canada. A total of 3,610 animals from 1,331 different farms were used in this cross-sectional cohort study. Geographic coordinates (latitude and longitude) were obtained for each farm and the 2 participating livestock auction markets. Calves' abnormal physical signs (APS) were noted upon arrival at the auction market as they were examined by trained research staff. The haversine distance between the farm and the auction market was evaluated using geographic coordinates and categorized. Generalized linear mixed models were used for statistical analyses. The main APS observed were ocular discharge (34.9%), abnormal hide cleanliness (21.2%), swollen navel (17.2%), dehydration score 1 (at least one of the 2 following clinical signs: persistent skin tent or sunken eye, 12.9%), and dehydration score 2 (both clinical signs mentioned above, 6.5%). Calves from farms located at greater distances from the auction markets (≥110 km) had a higher risk ratio [RR = 1.08; 95% confidence internal (CI) = 1.03, 1.13] for dehydration than those from lesser distances (0–25 km). During the summertime, a RR of 1.18 (95% CI = 1.15, 1.22) was observed for dehydration compared with wintertime. A 2-way interaction between estimated distance and season showed a higher prevalence of ocular discharge for calves from farms at distances greater than or equal to 110 km during the summer (RR = 1.11; 95% CI: 1.04, 1.20) than for calves from farms located at lesser distances (0–25 km). These results demonstrate that calves from farms located at greater distances from the auction markets had more APS, mainly during the summer. A better understanding of the transport conditions and interaction with management at the farm of origin is determinant to mitigate the impact of the journey on surplus calf health.
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- 2023
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31. Multisite Evaluation and Validation of Optical Genome Mapping for Prenatal Genetic Testing
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Levy, Brynn, Liu, Jie, Iqbal, M. Anwar, DuPont, Barbara, Sahajpal, Nikhil, Ho, Monique, Yu, Jingwei, Brody, Sam J., Ganapathi, Mythily, Rajkovic, Aleksandar, Smolarek, Teresa A., Boyar, Fatih, Bui, Peter, Dubuc, Adrian M., Kolhe, Ravindra, and Stevenson, Roger E.
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- 2024
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32. A Molecularly Integrated Grade for Meningioma
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Driver, Joseph, Hoffman, Samantha E, Tavakol, Sherwin, Woodward, Eleanor, Maury, Eduardo A, Bhave, Varun, Greenwald, Noah F, Nassiri, Farshad, Aldape, Kenneth, Zadeh, Gelareh, Choudhury, Abrar, Vasudevan, Harish N, Magill, Stephen T, Raleigh, David R, Abedalthagafi, Malak, Aizer, Ayal A, Alexander, Brian M, Ligon, Keith L, Reardon, David A, Wen, Patrick Y, Al-Mefty, Ossama, Ligon, Azra H, Dubuc, Adrian M, Beroukhim, Rameen, Claus, Elizabeth B, Dunn, Ian F, Santagata, Sandro, and Bi, Wenya Linda
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Biomedical and Clinical Sciences ,Clinical Sciences ,Oncology and Carcinogenesis ,Clinical Research ,Brain Disorders ,Brain Cancer ,Rare Diseases ,Human Genome ,Cancer ,Genetics ,Good Health and Well Being ,Adult ,Cohort Studies ,Humans ,Meningeal Neoplasms ,Meningioma ,Neoplasm Grading ,Neoplasm Recurrence ,Local ,Prognosis ,Retrospective Studies ,World Health Organization ,meningioma ,copy-number alterations ,Neurosciences ,Oncology & Carcinogenesis ,Oncology and carcinogenesis - Abstract
BackgroundMeningiomas are the most common primary intracranial tumor in adults. Clinical care is currently guided by the World Health Organization (WHO) grade assigned to meningiomas, a 3-tiered grading system based on histopathology features, as well as extent of surgical resection. Clinical behavior, however, often fails to conform to the WHO grade. Additional prognostic information is needed to optimize patient management.MethodsWe evaluated whether chromosomal copy-number data improved prediction of time-to-recurrence for patients with meningioma who were treated with surgery, relative to the WHO schema. The models were developed using Cox proportional hazards, random survival forest, and gradient boosting in a discovery cohort of 527 meningioma patients and validated in 2 independent cohorts of 172 meningioma patients characterized by orthogonal genomic platforms.ResultsWe developed a 3-tiered grading scheme (Integrated Grades 1-3), which incorporated mitotic count and loss of chromosome 1p, 3p, 4, 6, 10, 14q, 18, 19, or CDKN2A. 32% of meningiomas reclassified to either a lower-risk or higher-risk Integrated Grade compared to their assigned WHO grade. The Integrated Grade more accurately identified meningioma patients at risk for recurrence, relative to the WHO grade, as determined by time-dependent area under the curve, average precision, and the Brier score.ConclusionWe propose a molecularly integrated grading scheme for meningiomas that significantly improves upon the current WHO grading system in prediction of progression-free survival. This framework can be broadly adopted by clinicians with relative ease using widely available genomic technologies and presents an advance in the care of meningioma patients.
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- 2022
33. Degradation of Proteoglycans and Collagen in Equine Meniscal Tissues
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Julia Dubuc, Melodie Jil Schneider, Valerie Dubuc, Helene Richard, Maxime Pinsard, Stephane Bancelin, Francois Legare, Christiane Girard, and Sheila Laverty
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meniscus ,collagen ,proteoglycan ,intrasubstance ,degradation ,osteoarthritis ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Investigate meniscal extracellular matrix degradation. Equine menisci (n = 34 from 17 horses) were studied. Site-matched sections were cut and scored from three regions (ROIs; n = 102) and stained for histology, proteoglycan (safranin O and fast green), aggrecan, and collagen cleavage (NITEGE, DIPEN, and C1,2C antibodies, respectively). Picrosirius red and second harmonic generation microscopy were performed to investigate collagen ultrastructure. A total of 42 ROIs met the inclusion criteria and were included in the final analysis. The median (range) ROI histological score was 3 (0–9), providing a large spectrum of pathology. The median (range) proteoglycan score was 1 (0–3), representing superficial and central meniscal loss. The median (range) of DIPEN, NITEGE, and C1,2C scores was 1 (0–3), revealing immunostaining of the femoral and tibial surfaces. The proteoglycan scores exhibited significant positive associations with both histologic evaluation (p = 0.03) and DIPEN scores (p = 0.02). Additionally, a robust positive association (p = 0.007) was observed between the two aggrecanolysis indicators, NITEGE and DIPEN scores. A negative association (p = 0.008) was identified between NITEGE and histological scores. The C1,2C scores were not associated with any other scores. Picrosirius red and second harmonic generation microscopy (SHGM) illustrated the loss of the collagen matrix and structure centrally. Proteoglycan and collagen degradation commonly occur superficially in menisci and less frequently centrally. The identification of central meniscal proteoglycan and collagen degradation provides novel insight into central meniscal degeneration. However, further research is needed to elucidate the etiology and sequence of degradative events.
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- 2024
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34. Ensuring Online Learning Quality: Perspectives from the State University of New York
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Muller, Kristyn, Scalzo, Kim A., Pickett, Alexandra M., Dugan, Lawrence, Dubuc, Lisa, Simiele, Donna, McCabe, Ryan, and Pelz, William
- Abstract
As participation in online learning continues to expand, higher education institutions must implement policies and procedures to ensure quality at the course, program, and institution levels. In this paper, the authors describe a process that the State University of New York (SUNY) System implemented, utilizing the OLC Quality Scorecard, to help individual campuses examine the quality of their online offerings and develop strategies to support continuous improvement.
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- 2020
35. SARS-CoV-2 infection modifies the transcriptome of the megakaryocytes in the bone marrow
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Allaeys, Isabelle, Lemaire, Guillaume, Leclercq, Mickaël, Lacasse, Emile, Fleury, Maude, Dubuc, Isabelle, Gudimard, Leslie, Puhm, Florian, Tilburg, Julia, Stone, Andrew, Machlus, Kellie R., Droit, Arnaud, Flamand, Louis, and Boilard, Eric
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- 2024
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36. Evaluation of potassium monopersulfate footbath solution for controlling digital dermatitis in lactating dairy cattle. A randomized clinical trial.
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Ferraro, Salvatore, Rousseau, Marjolaine, Dufour, Simon, Dubuc, Jocelyn, Roy, Jean-Philippe, and Desrochers, André
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- 2024
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37. Epitranscriptome marks detection and localization of RNA modifying proteins in mammalian ovarian follicles
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Dubuc, Karine, Marchais, Mathilde, Gilbert, Isabelle, Bastien, Alexandre, Nenonene, Karen E., Khandjian, Edward W., Viger, Robert S., Delbes, Géraldine, and Robert, Claude
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- 2023
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38. Incidence and clinical manifestations of giant cell arteritis in Spain: results of the ARTESER register
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Santos Castañeda, Ricardo Blanco, Héctor Corominas, Patricia Carreira, Ivan Castellví, Eugenio De Miguel, Javier Narváez, Judit LLuch, Ivette Casafont-Solé, Jose María Pego, Lydia Abasolo, Carmen Larena, Francisco Ortiz-Sanjuán, Clara Moriano Morales, Elvira Díez Álvarez, Miguel Ángel González-Gay, Berta Magallares, Monica Ibañez Barcelo, Laura Garrido Courel, Vanesa Hernandez Hernandez, Patricia Moya Alvarado, Anne Riveros Frutos, Margarida Vasques Rocha, María Alcalde Villar, Antonio Juan Mas, Julio Sanchez, Joan Calvet, Clara Molina Almela, Amalia Rueda Cid, Cristina Campos Fernández, Carmen Riesco Bárcena, Patricia Moran Alvarez, Judit Font Urgelles, Alejandro Muñoz Jiménez, Delia Fernández-Lozano, Iñigo Hernández-Rodríguez, Marta Domínguez-Álvaro, Maite Silva-Díaz, Joaquín María Belzunegui, Eva Galíndez-Agirregoikoa, Vicente Aldaroso, Javier Loricera, Noemi Garrido-Puñal, Vanessa Andrea Navarro, Tarek Carlos Salman Monte, Trinidad Pérez Sandoval, Ismael González Fernández, Javier Mendizábal-Mateos, María Concepción Fito Manteca, Natividad del Val del Amo, Loreto Horcada Rubio, Inmaculada Paniagua Zudaire, Ricardo Gutiérrez Polo, Juliana Restrepo Vélez, Eduardo Loza Cortina, Elisa Fernández Fernández, Tomás Almorza, Leticia Léon Mateos, Luis Rodríguez Rodríguez, Pia Mercedes Lois Bermejo, Selene Labrada Arrabal, Susana Holgado Pérez, Jordi Camins, Javier Calvo Catalá, Rafael Benito Melero, Francisco Maceiras, Nair Pérez, Ceferino Barbazán, Irena Altabás, John Guzman, Paula Valentina Estrada Alarcón, Ana Milena Millán, AnaF Cruz Valenciano, Félix Cabero del Pozo, AnaBelén Rodríguez Cambrón, Cristina Macia Villa, Inmaculada Ros Vilamajó, Elide Toniolo, Ana Paula Cacheda, María Sagrario Bustabad Reyes, María García González, Alicia García Dorta, Jaime Calvo Allen, Miren Uriarte-Ecenarro, Cristina Valero Martínez, Esther F Vicente Rabaneda, Carlos García Porrúa, Carlota Laura Iñiguez Ubiaga, Noelia Álvarez Rivas, Tomás Ramón Vázquez Rodríguez, José Alberto Miranda Filloy, Amalia Sánchez-Andrade Fernández, Carlos Galisteo Lencastre Da Veiga, María Jesús García Villanueva, Marina Tortosa Cabañas, Marta Serrano Warleta, Aliuska Palomeque Vargas, Alberto Ruiz Román, Clara Aguilera Cros, JoséA Román Ivorra, Anderson Huaylla, Itziar Calvo Zorrilla, Jesús A Valero-Jaimes, Luis López Domínguez, Cesar Antonio Egues Dubuc, and Lucia Silva Fernández
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Medicine - Abstract
Objective This study aimed to estimate the incidence of giant cell arteritis (GCA) in Spain and to analyse its clinical manifestations, and distribution by age group, sex, geographical area and season.Methods We included all patients diagnosed with GCA between 1 June 2013 and 29 March 2019 at 26 hospitals of the National Health System. They had to be aged ≥50 years and have at least one positive results in an objective diagnostic test (biopsy or imaging techniques), meet 3/5 of the 1990 American College of Rheumatology classification criteria or have a clinical diagnosis based on the expert opinion of the physician in charge. We calculated incidence rate using Poisson regression and assessed the influence of age, sex, geographical area and season.Results We identified 1675 cases of GCA with a mean age at diagnosis of 76.9±8.3 years. The annual incidence was estimated at 7.42 (95% CI 6.57 to 8.27) cases of GCA per 100 000 people ≥50 years with a peak for patients aged 80–84 years (23.06 (95% CI 20.89 to 25.4)). The incidence was greater in women (10.06 (95% CI 8.7 to 11.5)) than in men (4.83 (95% CI 3.8 to 5.9)). No significant differences were found between geographical distribution and incidence throughout the year (p=0.125). The phenotypes at diagnosis were cranial in 1091 patients, extracranial in 337 patients and mixed in 170 patients.Conclusions This is the first study to estimate the incidence of GCA in Spain at a national level. We found a predominance among women and during the ninth decade of life with no clear variability according to geographical area or seasons of the year.
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- 2024
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39. Pharmacological targeting of the hyper-inflammatory response to SARS-CoV-2-infected K18-hACE2 mice using a cluster of differentiation 36 receptor modulator
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Jade Gauvin, David N. Huynh, Isabelle Dubuc, Catherine Lê, Rafaela Tugores, Nicolas Flamand, Louis Flamand, William D. Lubell, Huy Ong, and Sylvie Marleau
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coronavirus ,SARS-CoV-2 ,hyper-inflammation ,cytokine storm ,pneumonia ,macrophages ,Therapeutics. Pharmacology ,RM1-950 - Abstract
The scientific and medical community faced an unprecedented global health hazard that led to nearly 7 million deaths attributable to the rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. In spite of the development of efficient vaccines against SARS-CoV-2, many people remain at risk of developing severe symptoms as the virus continues to spread without beneficial patient therapy. The hyper-inflammatory response to SARS-CoV-2 infection progressing to acute respiratory distress syndrome remains an unmet medical need for improving patient care. The viral infection stimulates alveolar macrophages to adopt an inflammatory phenotype regulated, at least in part, by the cluster of differentiation 36 receptor (CD36) to produce unrestrained inflammatory cytokine secretions. We suggest herein that the modulation of the macrophage response using the synthetic CD36 ligand hexarelin offers potential as therapy for halting respiratory failure in SARS-CoV-2-infected patients.
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- 2024
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40. Registered report: Age-preserved semantic memory and the CRUNCH effect manifested as differential semantic control networks: An fMRI study.
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Niobe Haitas, Jade Dubuc, Camille Massé-Leblanc, Vincent Chamberland, Mahnoush Amiri, Tristan Glatard, Maximiliano Wilson, Yves Joanette, and Jason Steffener
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Medicine ,Science - Abstract
Semantic memory representations are generally well maintained in aging, whereas semantic control is thought to be more affected. To explain this phenomenon, this study tested the predictions of the Compensation-Related Utilization of Neural Circuits Hypothesis (CRUNCH), focusing on task demands in aging as a possible framework. The CRUNCH effect would manifest itself in semantic tasks through a compensatory increase in neural activation in semantic control network regions but only up to a certain threshold of task demands. This study compares 39 younger (20-35 years old) with 39 older participants (60-75 years old) in a triad-based semantic judgment task performed in an fMRI scanner while manipulating task demand levels (low versus high) through semantic distance. In line with the CRUNCH predictions, differences in neurofunctional activation and behavioral performance (accuracy and response times) were expected in younger versus older participants in the low- versus high-demand conditions, which should be manifested in semantic control Regions of Interest (ROIs). Our older participants had intact behavioral performance, as proposed in the literature for semantic memory tasks (maintained accuracy and slower response times (RTs)). Age-invariant behavioral performance in the older group compared to the younger one is necessary to test the CRUNCH predictions. The older adults were also characterized by high cognitive reserve, as our neuropsychological tests showed. Our behavioral results confirmed that our task successfully manipulated task demands: error rates, RTs and perceived difficulty increased with increasing task demands in both age groups. We did not find an interaction between age group and task demand, or a statistically significant difference in activation between the low- and high-demand conditions for either RTs or accuracy. As for brain activation, we did not find the expected age group by task demand interaction, or a significant main effect of task demand. Overall, our results are compatible with some neural activation in the semantic network and the semantic control network, largely in frontotemporoparietal regions. ROI analyses demonstrated significant effects (but no interactions) of task demand in the left and right inferior frontal gyrus, the left posterior middle temporal gyrus, the posterior inferior temporal gyrus and the prefrontal gyrus. Overall, our test did not confirm the CRUNCH predictions.
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- 2024
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41. Revisiting the two rhythm generators for respiration in lampreys
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Kianoush Missaghi, Jean-Patrick Le Gal, Julien Mercier, Martin Grover, Philippe-Antoine Beauséjour, Shannon Chartré, Omima Messihad, François Auclair, and Réjean Dubuc
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respiration ,respiratory generator ,neuroanatomy ,electrophysiology ,brainstem ,DAMGO ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 ,Human anatomy ,QM1-695 - Abstract
In lampreys, respiration consists of a fast and a slow rhythm. This study was aimed at characterizing both anatomically and physiologically the brainstem regions involved in generating the two rhythms. The fast rhythm generator has been located by us and others in the rostral hindbrain, rostro-lateral to the trigeminal motor nucleus. More recently, this was challenged by researchers reporting that the fast rhythm generator was located more rostrally and dorsomedially, in a region corresponding to the mesencephalic locomotor region. These contradictory observations made us re-examine the location of the fast rhythm generator using anatomical lesions and physiological recordings. We now confirm that the fast respiratory rhythm generator is in the rostro-lateral hindbrain as originally described. The slow rhythm generator has received less attention. Previous studies suggested that it was composed of bilateral, interconnected rhythm generating regions located in the caudal hindbrain, with ascending projections to the fast rhythm generator. We used anatomical and physiological approaches to locate neurons that could be part of this slow rhythm generator. Combinations of unilateral injections of anatomical tracers, one in the fast rhythm generator area and another in the lateral tegmentum of the caudal hindbrain, were performed to label candidate neurons on the non-injected side of the lateral tegmentum. We found a population of neurons extending from the facial to the caudal vagal motor nuclei, with no clear clustering in the cell distribution. We examined the effects of stimulating different portions of the labeled population on the respiratory activity. The rostro-caudal extent of the population was arbitrarily divided in three portions that were each stimulated electrically or chemically. Stimulation of either of the three sites triggered bursts of discharge characteristic of the slow rhythm, whereas inactivating any of them stopped the slow rhythm. Substance P injected locally in the lateral tegmentum accelerated the slow respiratory rhythm in a caudal hindbrain preparation. Our results show that the fast respiratory rhythm generator consists mostly of a population of neurons rostro-lateral to the trigeminal motor nucleus, whereas the slow rhythm generator is distributed in the lateral tegmentum of the caudal hindbrain.
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- 2024
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42. Actor Critic Agents for Wind Farm Control.
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Claire Bizon Monroc, Ana Busic, Donatien Dubuc, and Jiamin Zhu
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- 2023
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43. Fertility of Immigrants: S. Dubuc
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Dubuc, Sylvie, Bean, Frank D., editor, and Brown, Susan K, editor
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- 2023
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44. Rationale and Design of the Randomized Bayesian Multicenter COME-TAVI Trial in Patients With a New Onset Left Bundle Branch Block
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Lena Rivard, MD, MSc, Isabelle Nault, MD, Andrew D. Krahn, MD, Benoit Daneault, MD, Jean-Francois Roux, MD, Madhu Natarajan, MD, Jeffrey S. Healey, MD, MSc, Kenneth Quadros, MD, Roopinder K. Sandhu, MD, MPH, Remi Kouz, MD, Isabelle Greiss, MD, Peter Leong-Sit, MD, Jean Baptiste Gourraud, MD, Walid Ben Ali, MD, PhD, Anita Asgar, MD, Msc, Martin Aguilar, MD, PhD, Raoul Bonan, MD, Julia Cadrin-Tourigny, MD, PhD, Raymond Cartier, MD, Jean-Francois Dorval, MD, Marc Dubuc, MD, Nicolas Dürrleman, MD, MSc, Katia Dyrda, MD, Peter Guerra, MD, Marina Ibrahim, MD, MSc, Reda Ibrahim, MD, Laurent Macle, MD, Blandine Mondesert, MD, Emmanuel Moss, MD, MSc, Alexandre Raymond-Paquin, MD, Denis Roy, MD, Rafik Tadros, MD, PhD, Bernard Thibault, MD, Mario Talajic, MD, Anna Nozza, MSc, Marie-Claude Guertin, PhD, and Paul Khairy, MD, PhD
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Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Patients with new-onset left bundle branch block (LBBB) after transcatheter aortic valve implantation (TAVI) are at risk of developing delayed high-degree atrioventricular block. Management of new-onset LBBB post-TAVI remains controversial. In the Comparison of a Clinical Monitoring Strategy Versus Electrophysiology-Guided Algorithmic Approach in Patients With a New LBBB After TAVI (COME-TAVI) trial, consenting patients with new-onset LBBB that persists on day 2 after TAVI, meeting exclusion/inclusion criteria, are randomized to an electrophysiological study (EPS)-guided approach or 30-day electrocardiographic monitoring. In the EPS-guided approach, patients with a His to ventricle (HV) interval ≥ 65 ms undergo permanent pacemaker implantation. Patients randomized to noninvasive monitoring receive a wearable continuous electrocardiographic recording and transmitting device for 30 days. Follow-up will be performed at 3, 6, and 12 months. The primary endpoint is a composite outcome designed to capture net clinical benefit. The endpoint incorporates major consequences of both strategies in patients with new-onset LBBB after TAVI, as follows: (i) sudden cardiac death; (ii) syncope; (iii) atrioventricular conduction disorder requiring a pacemaker (for a class I or IIa indication); and (iv) complications related to the pacemaker or EPS. The trial incorporates a Bayesian design with a noninformative prior, outcome-adaptive randomization (initially 1:1), and 2 prespecified interim analyses once 25% and 50% of the anticipated number of primary endpoints are reached. The trial is event-driven, with an anticipated upper limit of 452 patients required to reach 77 primary outcome events over 12 months of follow-up. In summary, the aim of this Bayesian multicentre randomized trial is to compare 2 management strategies in patients with new-onset LBBB post-TAVI—an EPS-guided approach vs noninvasive 30-day monitoring. Trial registration number: NCT03303612. Résumé: Les patients chez qui un bloc de branche gauche (BBG) est récemment apparu à la suite de l’implantation valvulaire aortique par cathéter (IVAC) présentent un risque de bloc auriculoventriculaire de haut degré tardif. La prise en charge d’un BBG récemment apparu après une IVAC demeure controversée. Dans le cadre de l’essai COME-TAVI (Comparison of a Clinical Monitoring Strategy Versus Electrophysiology-Guided Algorithmic Approach in Patients With a New LBBB After TAVI, ou comparaison d’une stratégie de surveillance clinique, par rapport à une approche guidée par étude électrophysiologique et fondée sur un algorithme, chez des patients présentant un BBG d’apparition récente à la suite d’une IVAC), des patients qui présentent un BBG d’apparition récente persistant le 2e jour après une IVAC, qui répondent aux critères d’admissibilité et qui ont donné leur consentement sont répartis aléatoirement pour être suivis à l’aide d’une approche guidée par une étude électrophysiologique (EEP) ou faire l’objet d’une surveillance électrocardiographique d’une durée de 30 jours. Un stimulateur cardiaque est implanté chez les patients du groupe de l’EEP dont l’intervalle HV (temps de conduction dans le tronc du faisceau de His jusqu’aux ventricules) est ≥ 65 ms. Les patients du groupe de surveillance non invasive reçoivent un dispositif portable d’enregistrement et de transmission continue de données électrocardiographiques pour une période de 30 jours. Le suivi sera réalisé aux 3e, 6e et 12e mois. Le critère d’évaluation principal est un paramètre composite conçu afin de saisir le bienfait clinique net. Il comprend les conséquences majeures des deux stratégies chez les patients présentant un BBG d’apparition récente après une IVAC, comme suit : (i) mort subite d’origine cardiaque; (ii) syncope; (iii) trouble de la conduction auriculoventriculaire nécessitant la pose d’un stimulateur cardiaque (pour une indication de classe I ou IIa); et (iv) complications relatives au stimulateur cardiaque ou à l’EEP. L’essai intègre une conception bayésienne avec une répartition aléatoire (dans un rapport initial de 1:1) antérieure non informative adaptée aux résultats et deux analyses intermédiaires définies au préalable lorsque 25 % et 50 % du nombre anticipé des critères d’évaluation principaux seront atteints. L’essai est axé sur les événements, et la limite supérieure anticipée pour atteindre 77 événements relatifs aux critères d’évaluation principaux sur 12 mois de suivi est de 452 patients. En résumé, l’objectif de cet essai bayésien multicentrique à répartition aléatoire est de comparer deux stratégies de prise en charge de patients présentant un BBG d’apparition récente après une IVAC, soit une approche guidée par une EEP, par rapport à une surveillance non invasive de 30 jours. Trial registration number: NCT03303612.
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- 2023
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45. Crack length directivity effects on guided-wave acoustic emission: Numerical investigation of radiation patterns
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Dubuc, Brennan
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- 2024
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46. Psychothérapie assistée par psychédéliques (PAP) : le modèle genevois
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Seragnoli, Federico, Thorens, Gabriel, Penzenstadler, Louise, Furtado, Leonice, Buchard, Albert, Bachmann, Silke, Iuga, Radu, Khatcherian, Eugénie, Nowotarski, Adam, Sabe, Michel, Richard-Lepouriel, Hélène, Glangetas, Alban, Girani, Léa, Anastasova, Raya, Girardet, Alexis, Yang, Ray, Lécureux, Léo, Alaux, Sylvie, Mabilais, Cedric, Amberger, Caroline, Dubuc, Charles, Szczesniak, Laurent, Blanc, Johanna, Tudor, Maria, Elrhaoussi, Akram, de Chambrier, Pablo, Petignat, Cyril, Rochat, Lucien, Briefer, Jean-François, Rothen, Stéphane, Aboulafia Brakha, Tatiana, Qusaj, Visar, Cheminal, Ségolen, Billieux, Joël, and Zullino, Daniele
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- 2024
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47. Twists and Turns from 'Tumor in Tumor' Profiling: Surveillance of CLL leads to detection of a lung adenocarcinoma, whose genomic characterization alters the original hematologic diagnosis
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Terraf, Panieh, Sholl, Lynette M, Davids, Matthew S, Awad, Mark M, Garcia, Elizabeth P, MacConaill, Laura E, Dal Cin, Paola, Kim, Annette, Lindeman, Neal I, Stachler, Matthew, Hwang, David H, and Dubuc, Adrian M
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Biomedical and Clinical Sciences ,Clinical Sciences ,Oncology and Carcinogenesis ,Rare Diseases ,Lymphoma ,Lung ,Cancer ,Genetics ,Lung Cancer ,Hematology ,Good Health and Well Being ,Adenocarcinoma of Lung ,Aged ,Biomarkers ,Tumor ,Diagnostic Errors ,Female ,Gene Expression Profiling ,Gene Rearrangement ,High-Throughput Nucleotide Sequencing ,Humans ,In Situ Hybridization ,Fluorescence ,Leukemia ,Lymphocytic ,Chronic ,B-Cell ,Lung Neoplasms ,Lymphoma ,Mantle-Cell ,Neoplasms ,Multiple Primary ,Positron Emission Tomography Computed Tomography ,chronic lymphatic leukemia ,hematological neoplasm ,lung adenocarcinoma ,Pharmacology and pharmaceutical sciences - Abstract
Comprehensive characterization of somatic genomic alterations has led to fundamental shifts in our understanding of tumor biology. In clinical practice, these studies can lead to modifications of diagnosis and/or specific treatment implications, fulfilling the promise of personalized medicine. Herein, we describe a 78-yr-old woman under surveillance for long-standing untreated chronic lymphocytic leukemia (CLL). Molecular studies from a peripheral blood specimen revealed a TP53 p.V157F mutation, whereas karyotype and fluorescence in situ hybridization (FISH) identified a 17p deletion, trisomy 12, and no evidence of IGH-CCND1 rearrangement. Positron emission tomography-computed tomography scan identified multistation intra-abdominal lymphadenopathy and a pulmonary nodule, and subsequent pulmonary wedge resection confirmed the presence of a concurrent lung adenocarcinoma. Targeted next-generation sequencing of the lung tumor identified an EGFR in-frame exon 19 deletion, two TP53 mutations (p.P152Q, p.V157F), and, unexpectedly, a IGH-CCND1 rearrangement. Follow-up immunohistochemistry (IHC) studies demonstrated a cyclin D1-positive lymphoid aggregate within the lung adenocarcinoma. The presence of the TP53 p.V157F mutation in the lung resection, detection of an IGH-CCND1 rearrangement, and cyclin D1 positivity by IHC led to revision of the patient's hematologic diagnosis and confirmed the extranodal presence of mantle cell lymphoma within the lung mass, thus representing a "tumor in tumor." Manual review of the sequencing data suggested the IGH-CCND1 rearrangement occurred via an insertional event, whose size precluded detection by original FISH studies. Thus, routine imaging for this patient's known hematologic malignancy led to detection of an unexpected solid tumor, whose subsequent precision medicine studies in the solid tumor redefined the original hematological diagnosis.
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- 2021
48. Epitranscriptome marks detection and localization of RNA modifying proteins in mammalian ovarian follicles
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Karine Dubuc, Mathilde Marchais, Isabelle Gilbert, Alexandre Bastien, Karen E. Nenonene, Edward W. Khandjian, Robert S. Viger, Géraldine Delbes, and Claude Robert
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RNA methylation ,Oocytes ,Transzonal projections ,Somatic cells ,RNA modifying enzyme ,Epitranscriptome ,Gynecology and obstetrics ,RG1-991 - Abstract
Abstract Background Most of the resources that support the early development of the embryo are stored in the oocyte. Clearing of maternal resources and activation of the embryonic genome to produce its own mRNA transcripts marks the maternal-to-embryo transition. Dependence on stored mRNA can last from a few hours to several days, depending on animal species. The mechanisms regulating stabilization and recruitment of stored maternal transcripts have not yet been described in full detail but are known to involve reversible polyadenylation and modulation of 3’UTR-mediated elements. RNA epigenetic modifications, new players in this field, have an important role in RNA regulation and stabilization. Results The objectives of this study were first to determine if some of post-transcriptional methylation of stored mRNA is greater in oocytes than in somatic cells. We found that m6A, known to be the most prevalent and involved in various aspects of RNA metabolism and physiological functions, is particularly abundant in porcine oocyte mRNA compared to liver used as a somatic tissue reference. The second objective was to compare the epitranscriptome machinery, such as methyltransferases (“writers”), binding proteins (“readers”) and demethylases (“erasers”) catalyzing the different process, in follicles and oocytes of different mammalian species by immunofluorescence and confocal microscopy. The expression and localization patterns of these proteins differ between mice, pigs and cows ovaries and oocytes. m5C-associated proteins were generally less abundant. In contrast, m6A-associated proteins were expressed strongly during the early and late stages of folliculogenesis. Transzonal projections were found to contain more granules bearing the m5C mark in mice but both m5C and m6A methylation marks in association with mature oocytes of pigs and cows. Eraser proteins showed the greatest interspecies diversity in terms of distribution in the germinal tissues. Conclusions So far, few studies have looked at the oocyte and ovarian epitranscriptomic profile. Our findings indicate that a hitherto unrecognized species-specific layer of transcript regulation occurs at the RNA level and might be consequential during the oocyte transcriptional silencing period.
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- 2023
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49. Pain relief devoid of opioid side effects following central action of a silylated neurotensin analog
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Tétreault, Pascal, Besserer-Offroy, Élie, Brouillette, Rebecca L., René, Adeline, Murza, Alexandre, Fanelli, Roberto, Kirby, Karyn, Parent, Alexandre J., Dubuc, Isabelle, Beaudet, Nicolas, Côté, Jérôme, Longpré, Jean-Michel, Martinez, Jean, Cavelier, Florine, and Sarret, Philippe
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Quantitative Biology - Biomolecules ,Quantitative Biology - Quantitative Methods - Abstract
Neurotensin (NT) exerts naloxone-insensitive antinociceptive action through its binding to both NTS1 and NTS2 receptors and NT analogs provide stronger pain relief than morphine on a molecular basis. Here, we examined the analgesic/adverse effect profile of a new NT(8-13) derivative denoted JMV2009, in which the Pro10 residue was substituted by a silicon-containing unnatural amino acid silaproline. We first report the synthesis and in vitro characterization (receptor-binding affinity, functional activity and stability) of JMV2009. We next examined its analgesic activity in a battery of acute, tonic and chronic pain models. We finally evaluated its ability to induce adverse effects associated with chronic opioid use, such as constipation and analgesic tolerance or related to NTS1 activation, like hypothermia. In in vitro assays, JMV2009 exhibited high binding affinity for both NTS1 and NTS2, improved proteolytic resistance as well as agonistic activities similar to NT, inducing sustained activation of p42/p44 MAPK and receptor internalization. Intrathecal injection of JMV2009 produced dose-dependent antinociceptive responses in the tail-flick test and almost completely abolished the nociceptive-related behaviors induced by chemical somatic and visceral noxious stimuli. Likewise, increasing doses of JMV2009 significantly reduced tactile allodynia and weight bearing deficits in nerve-injured rats. Importantly, chronic agonist treatment did not result in the development of analgesic tolerance. Furthermore, JMV2009 did not cause constipation and was ineffective in inducing hypothermia. These findings suggest that NT drugs can act as an effective opioid-free medication for the management of pain or can serve as adjuvant analgesics to reduce the opioid adverse effects., Comment: This is the post-print (accepted) version of the following article: T\'etreault P, et al. (2020), Eur J Pharmacol. doi: 10.1016/j.ejphar.2020.173174, which has been accepted and published in final form at https://doi.org/10.1016/j.ejphar.2020.173174
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- 2020
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50. Evaluación espirométrica en adolescentes que usan cigarrillos electrónicos
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Sofía Isea López, José Octavio Isea Dubuc, Luis Carlos Morillo, Alejandro Mondolfi, Agustín Acuña, María Eugenia García, Ibis Ortíz, Adriana Sánchez, Cecilia Oropeza, and Giorsari Quezada
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Cigarrillos electrónicos ,Vapeadores ,Espirometría ,Función pulmonar ,Adolescentes ,Medicine - Abstract
Introducción: El uso de cigarrillos electrónicos o vapeadores (CE/V), ha representado un problema de salud mundial en la última década. Su uso por parte de la población adolescente resulta de gran preocupación debido a que se desconocen los efectos a largo plazo que estos pudieran ocasionar. La información sobre la situación y prevalencia, del uso de CE/V en Venezuela no ha sido documentada y el uso de estos dispositivos puede ocasionar adicción a nicotina que se genera rápidamente en jóvenes, pudiendo facilitar el uso de tabaco de combustión u otras drogas ilícitas. Objetivo: Determinar patrones de respiración alterados y presencia de cambios en la vía aérea en los adolescentes que usan CE/V. Métodos: Estudio observacional, descriptivo, que abarcó un grupo de adolescentes de secundaria de un colegio de Caracas. Se realizó una encuesta interrogando aspectos relacionados al uso de CE/V, luego examen físico y espirometría. Se compararon medias numéricas a través de la T de student y valor de P. Resultados: El uso de CE/V en el grupo de estudio era frecuente. Se evidenció la presencia de conductas de riesgo asociadas como la ingesta de alcohol. En la espirometría los usuarios de CE/V tenían valores de FEV1/FVC y FEF 25-75% menores en relación a los no usuarios aunque no existió una diferencia significativa entre ambos grupos. Conclusión: El uso de CE/V constituye un riesgo importante para los adolescentes. Las alteraciones producidas por CE/V son progresivas y a largo plazo por lo que es importante el seguimiento cercano de estos pacientes.
- Published
- 2023
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