Search

Background. Treatment of diabetic neuropathic pain (DNP) continues to be a major challenge, and underlying mechanisms of DNP remain elusive. We investigated treatment effects of B vitamins on DPN- and DNP-associated alterations of neurochemical signaling in the nociceptive dorsal root ganglion (DRG) neurons and the spinal cord in rats. Methods. DNP was produced in male, adult, Sprague Dawley rats by single i.p. streptozotocin (STZ). Western blot analysis and immunohistochemistry were used to analyze protein expressions in DRG and ELISA to measure the proinflammatory cytokines in the spinal cord. Behaviorally expressed DNP was determined by measuring the sensitivity of hindpaw skin to mechanical and thermal stimulation. Results. There were 87.5% (77/88) rats which developed high blood glucose within 1-2 weeks following STZ injection. Of which, 70.13% (n = 54/77) animals exhibited DNP manifested as mechanical allodynia and/or thermal hyperalgesia. Intraperitoneal administration of vitamins B1/B6/B12 (100/100/2 mg/kg, one or multiple doses) significantly attenuated DNP without affecting the blood glucose. Expressions of P2X3 and TRPV1 in CGRP-positive and IB4-positive DRG neurons as well as the interleukin-1β, tumor necrosis factor-α, and nerve growth factor in the lumbar spinal cord were greatly increased in DNP rats. Such DNP-associated neurochemical alterations were also greatly suppressed by the B-vitamin treatment. Conclusions. B-vitamin treatment can greatly suppress chronic DNP and DNP-associated increased activities of P2X3 and TRPV1 in DRG and the spinal proinflammatory cytokines, which may contribute to the pathogenesis of DNP. Systematic administration of B vitamins can be a strategy for DNP management in clinic.

AIM: To investigate the effect of recombinant bovine basic fibroblast growth factor(rb-bFGF)eye drops and hydroxyl indican eye drops on tear film stability and dry eye symptoms after age-related cataract surgery. METHODS: Totally 80 patients with 80 affected eyes undergoing age-related cataract surgery in our hospital from January 2015 to October 2016 were selected as study subjects, and they were randomly divided into control group and experimental group with 40 patients(40 affected eyes)in each group. The two groups were treated with hydroxyl indican eye drops and rb-bFGF eye drops, respectively. The clinical curative effect, inflammation related factors \〖interleukin 6(IL-6), tumor necrosis factor α(TNF-α)\〗, Schirmer test(SⅠt), break-up time(BUT)of tear film, corneal sodium fluorescein staining(FL)and scores of dry eye symptoms in the two groups were observed. RESULTS: The total treatment effective rate of experimental group after treatment was significantly higher than that of the control group(90.0% vs 72.5%; χ2=4.021, PP>0.05). After treatment, IL-6, TNF-α, SⅠt, FL score and scores of dry eye symptoms in two groups significantly decreased while BUT significantly increased(PPCONCLUSION: In the treatment of patients after age-related cataract after surgery, rb-bFGF eye drops has more advantages over hydroxyl indican eye drops in regulating the expression of inflammatory factors, improving the tear film stability and relieving dry eye symptoms.

Single-phase magnesium molybdate, MgMoO4, is successfully synthesized by a facile sol-gel method. Attributed to the multielectron reaction and the synergistic effect of the elements molybdenum (Mo) and magnesium (Mg), the MgMoO4 electrode exhibits excellent electrochemical properties. After activation, benefiting from the decrease in particle size and the uniform nanosphere morphology, the MgMoO4 electrodes can deliver a stable high specific capacity of about 1060 mA h g−1 at a current density of 100 mA g−1 after 600 cycles. Based on the important role of the activation process, electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM) and scan rate cyclic voltammetry (CV) measurement methods were employed to reveal the effect of the activation process on the electrochemical behavior of the electrode material. Furthermore, by combining the in situ X-ray diffraction (XRD) and ex situ X-ray photoelectron spectroscopy (XPS) results, we illustrate the lithium storage mechanism of the MgMoO4 electrode in detail. [ABSTRACT FROM AUTHOR]