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159 results on '"Duan, Canxing"'

7. An Emerging Disease of Chickpea, Basal Stem Rot Caused by Diaporthe aspalathi in China.

Author

Wang, Danhua, Deng, Dong, Zhan, Junliang, Wu, Wenqi, Duan, Canxing, Sun, Suli, and Zhu, Zhendong

Subjects
MUNG bean, PLANT diseases, LATHYRUS, COWPEA, LEGUMES
Abstract

Chickpea (Cicer arietinum L.) is an important legume crop worldwide. An emerging disease, basal stem rot with obvious wilt symptoms, was observed in the upper part of chickpea plants during the disease survey in Qiubei County of Yunnan Province. Three fungal isolates (ZD36-1, ZD36-2, and ZD36-3) were obtained from the diseased tissue of chickpea plants collected from the field. Those isolates were morphologically found to be similar to Diaporthe aspalathi. Molecular sequence analyses of multiple gene regions (ITS, tef1, tub2, cal, and his3) indicated that the three isolates showed a high identity with D. aspalathi. Pathogenicity and host range tests of the isolates were performed on the original host chickpea and eight other legume crops. The isolates were strongly pathogenic to chickpea and appeared highly pathogenic to soybean, cowpea, and mung bean; moderated or mild pathogenic to adzuki bean and common bean; however, the isolates did not cause symptoms on grass pea (Lathyrus sativus). Diaporthe aspalathi was previously reported as a main pathogen causing the southern stem canker in soybean. To our knowledge, this is the first report of D. aspalathi inducing basal stem rot on chickpea worldwide. [ABSTRACT FROM AUTHOR]

Published
2024
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8. Transcriptomic and Metabolomic Analyses Reveal the Role of Phenylalanine Metabolism in the Maize Response to Stalk Rot Caused by Fusarium proliferatum

Author

Sun, Jianjun, primary, Wang, Yanzhao, additional, Zhang, Xingrui, additional, Cheng, Zeqiang, additional, Song, Yinghui, additional, Li, Huimin, additional, Wang, Na, additional, Liu, Shen, additional, Cao, Zijia, additional, Li, Hongxia, additional, Zheng, Wanying, additional, Duan, Canxing, additional, and Cao, Yanyong, additional

Published
2024
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10. Genome-wide association study of maize resistance to Pythium aristosporum stalk rot

Author

Hou, Mengwei, primary, Cao, Yanyong, additional, Zhang, Xingrui, additional, Zhang, Shulin, additional, Jia, Tengjiao, additional, Yang, Jiwei, additional, Han, Shengbo, additional, Wang, Lifeng, additional, Li, Jingjing, additional, Wang, Hao, additional, Zhang, Lili, additional, Wu, Xiaolin, additional, Duan, Canxing, additional, and Li, Huiyong, additional

Published
2023
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16. A novel pathogen Fusarium cuneirostrumcausing common bean (Phaseolus vulgaris) root rot in China

Author

Deng, Dong, Wu, Wenqi, Duan, Canxing, Sun, Suli, and Zhu, Zhendong

Abstract

Several fungal pathogens cause root rot of common bean, among which Fusariumspp. are the most common pathogens causing Fusarium root rot (FRR) worldwide. FRR has been becoming an increasingly severe disease of common bean in China, but the species of Fusariumspp. have remained unclear. Thus, this study was performed to identify the pathogen causing common bean root rot in Liangcheng County, Inner Mongolia, China. Nineteen Fusarium-like isolates were obtained after pathogen isolation and purification. The pathogenicity test indicated that eight isolates caused severe disease symptoms on common bean, while 11 other isolates were not pathogenic. The eight pathogenic isolates, FCL1–FCL8, were identified as Fusarium cuneirostrumby morphological characterization and phylogenetic analysis using partial sequences of EF-1α, ITS, 28S, and IGS regions. Host range test showed that the representative F. cuneirostrumisolate FCL3 was also pathogenic to mung bean, while not pathogenic to adzuki bean, chickpea, cowpea, faba bean, pea, and soybean. Moreover, 50 common bean and 50 mung bean cultivars were screened for resistance to FRR, and seven highly resistant or resistant cultivars of common bean were identified, while no resistant cultivars of mung bean were screened. This study revealed that F. cuneirostrumwas one of common bean FRR pathogens in Inner Mongolia and it could induce mung bean root rot as well. To our knowledge, this is the first report of F. cuneirostrumcausing FRR of common bean in China.

Published
2024
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17. Single‐cell RNA sequencing profiles reveal cell type‐specific transcriptional regulation networks conditioning fungal invasion in maize roots.

Author

Cao, Yanyong, Ma, Juan, Han, Shengbo, Hou, Mengwei, Wei, Xun, Zhang, Xingrui, Zhang, Zhanyuan J., Sun, Suli, Ku, Lixia, Tang, Jihua, Dong, Zhenying, Zhu, Zhendong, Wang, Xiaoming, Zhou, Xiaoxiao, Zhang, Lili, Li, Xiangdong, Long, Yan, Wan, Xiangyuan, and Duan, Canxing

Subjects
GENE regulatory networks, GENETIC transcription regulation, RNA sequencing, CELL determination, CORN diseases, CORN, GENE expression
Abstract

Summary: Stalk rot caused by Fusarium verticillioides (Fv) is one of the most destructive diseases in maize production. The defence response of root system to Fv invasion is important for plant growth and development. Dissection of root cell type‐specific response to Fv infection and its underlying transcription regulatory networks will aid in understanding the defence mechanism of maize roots to Fv invasion. Here, we reported the transcriptomes of 29 217 single cells derived from root tips of two maize inbred lines inoculated with Fv and mock condition, and identified seven major cell types with 21 transcriptionally distinct cell clusters. Through the weighted gene co‐expression network analysis, we identified 12 Fv‐responsive regulatory modules from 4049 differentially expressed genes (DEGs) that were activated or repressed by Fv infection in these seven cell types. Using a machining‐learning approach, we constructed six cell type‐specific immune regulatory networks by integrating Fv‐induced DEGs from the cell type‐specific transcriptomes, 16 known maize disease‐resistant genes, five experimentally validated genes (ZmWOX5b, ZmPIN1a, ZmPAL6, ZmCCoAOMT2, and ZmCOMT), and 42 QTL or QTN predicted genes that are associated with Fv resistance. Taken together, this study provides not only a global view of maize cell fate determination during root development but also insights into the immune regulatory networks in major cell types of maize root tips at single‐cell resolution, thus laying the foundation for dissecting molecular mechanisms underlying disease resistance in maize. [ABSTRACT FROM AUTHOR]

Published
2023
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21. A field-deployable method for single and multiplex detection of DNA or RNA from pathogens using Cas12 and Cas13

Author

Li, Lina, Duan, Canxing, Weng, Jianfeng, Qi, Xiantao, Liu, Changlin, Li, Xinhai, Zhu, Jinjie, and Xie, Chuanxiao

Subjects
nucleic acid detection, LbCas12a, DNA, RfxCas13d, AsCas12a, Endonucleases, General Biochemistry, Genetics and Molecular Biology, Fusarium head blight, Nucleic Acids, maize ear rot, RNA, LwaCas13a, rice black-streaked dwarf virus (RBSDV), CRISPR-Cas Systems, General Agricultural and Biological Sciences, Research Paper, General Environmental Science
Abstract

For some Cas nucleases, trans-cleavage activity triggered by CRISPR/Cas-mediated cis-cleavage upon target nucleic acid recognition has been explored for diagnostic detection. Portable single and multiplex nucleic acid-based detection is needed for crop pathogen management in agriculture. Here, we harnessed and characterized RfxCas13d as an additional CRISPR/Cas nucleic acid detection tool. We systematically characterized AsCas12a, LbCas12a, LwaCas13a, and RfxCas13d combined with isothermal amplification to develop a CRISPR/Cas nucleic acid-based tool for single or multiplex pathogen detection. Our data indicated that sufficient detection sensitivity was achieved with just a few copies of DNA/RNA targets as input. Using this tool, we successfully detected DNA from Fusarium graminearum and Fusarium verticillioides and RNA from rice black-streaked dwarf virus in crude extracts prepared in the field. Our method, from sample preparation to result readout, could be rapidly and easily deployed in the field. This system could be extended to other crop pathogens, including those that currently lack a detection method and have metabolite profiles that make detection challenging. This nucleic acid detection system could also be used for single-nucleotide polymorphism genotyping, transgene detection, and qualitative detection of gene expression in the field. Supporting Information The supporting information is available online at 10.1007/s11427-021-2028-x. The supporting materials are published as submitted, without typesetting or editing. The responsibility for scientific accuracy and content remains entirely with the authors.

Published
2021

22. Identification of a Fusarium ear rot resistance gene in maize by QTL mapping and RNA sequencing

Author

Xia, Yusheng, Wang, Baobao, Zhu, Lihong, Wu, Wenqi, Sun, Suli, Zhu, Zhendong, Li, Xinhai, Weng, Jianfeng, and Duan, Canxing

Subjects
Plant Science
Abstract

Fusarium ear rot (FER) caused by Fusarium verticillioides is a prevalent maize disease. To comprehensively characterize the genetic basis of the natural variation in FER resistance, a recombinant inbred line (RIL) population was used to map quantitative trait loci (QTL) for FER resistance. A total of 17 QTL were identified by linkage mapping in eight environments. These QTL were located on six chromosomes and explained 3.88–15.62% of the total phenotypic variation. Moreover, qFER1.03 had the strongest effect and accounted for 4.98–15.62% of the phenotypic variation according to analyses of multiple environments involving best linear unbiased predictions. The chromosome segment substitution lines (CSSLs) derived from a cross between Qi319 (donor parent) and Ye478 (recurrent parent) were used to verify the contribution of qFER1.03 to FER resistance. The line CL171, which harbored an introgressed qFER1.03, was significantly resistant to FER. Further fine mapping of qFER1.03 revealed that the resistance QTL was linked to insertion/deletion markers InDel 8 and InDel 2, with physical distances of 43.55 Mb and 43.76 Mb, respectively. Additionally, qFER1.03 differed from the previous resistance QTL on chromosome 1. There were three annotated genes in this region. On the basis of the RNA-seq data, which revealed the genes differentially expressed between the FER-resistant Qi319 and susceptible Ye478, GRMZM2G017792 (MPK3) was preliminarily identified as a candidate gene in the qFER1.03 region. The Pr-CMV-VIGS system was used to decrease the GRMZM2G017792 expression level in CL171 by 34–57%, which led to a significant decrease in FER resistance. Using RIL and CSSL populations combined with RNA-seq and Pr-CMV-VIGS, the candidate gene can be dissected effectively, which provided important gene resource for breeding FER-resistant varieties.

Published
2022

39. Engineering null mutants in ZmFER1 confers resistance to ear rot caused by Fusarium verticillioides in maize.

Author

Liu, Changlin, Kong, Ming, Zhu, Jinjie, Qi, Xiantao, Duan, Canxing, and Xie, Chuanxiao

Subjects
GIBBERELLA fujikuroi, CORN, EAR, LOCUS (Genetics)
Abstract

Keywords: maize; CRISPR/Cas9; Fusarium ear rot; ZmFER1 EN maize CRISPR/Cas9 Fusarium ear rot ZmFER1 2045 2047 3 11/01/22 20221101 NES 221101 I Fusarium i ear rot (FER), a fungal disease caused by I Fusarium i spp., leads to great yield losses to maize worldwide and to mycotoxin contamination, which seriously threatens human and animal health (Yao I et al i ., 2020). Maize, CRISPR/Cas9, Fusarium ear rot, ZmFER1. [Extracted from the article]

Published
2022
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40. Occurrence and identification of Nothophoma quercina causing brown spot of jujube in China

Author

Bai Jianyu, Duan Canxing, Shi Yanjiang, and Wang Xiaoming

Subjects
0106 biological sciences, 0301 basic medicine, Spots, Inoculation, 030106 microbiology, Plant Science, Fungus, Biology, biology.organism_classification, 01 natural sciences, food.food, Brown spot, 03 medical and health sciences, food, Ziziphus jujuba, Botany, Phoma, Internal transcribed spacer, Pycnidium, Agronomy and Crop Science, 010606 plant biology & botany
Abstract

A new disease was observed on jujube (Ziziphus jujuba Mill.) in Awti county, Xinjiang, China, in 2014. The symptoms on infected leaves consisted of spots which were tan in the centre, with black pycnidia, surrounded by dark-brown to black margins, and yellow halos. Infected fruit became dark brown to black, and were covered by small pycnidia. The causal agent was isolated from infected leaves and fruit. Pathogenicity tests showed that the fungus could infect jujube fruits, which developed the same symptoms under artificial inoculation conditions as those observed in the field. The fungus was identified based on morphological and cultural characteristics as Nothophoma quercina (Syd) Q. Chen & L. Cai. Identification was confirmed by combining multi-locus phylogenetic analyses based on the internal transcribed spacer regions (ITS 1 and 2) and intervening 5.8S nrDNA, and partial gene regions of β-tubulin (TUB 2). This is the first report of brown spot of jujube caused by Nothophoma quercina (Phoma fun...

Published
2016

47. Wildfire, a new bacterial disease of mung bean, caused by Pseudomonas syringaepv. tabaci

Author

Sun, Suli, Liu, Changyou, Duan, Canxing, and Zhu, Zhendong

Abstract

Mung bean (Vigna radiateL.) is a major legume crop and widely planted in China. A new bacterial foliar disease similar to wildfire was observed on the mung bean plants. This study was conducted to determine the causal agent inducing wildfire-like symptoms on mung bean. The bacterial isolates obtained from mung bean were performed to detailed identification. All isolates showed the similar morphology and molecular characteristics to those of Pseudomonas syringae. By pathogenicity tests, the isolates were strongly pathogenic to mung bean and tobacco, a common host of wildfire disease. Based on the morphological and molecular characteristics, including the specific detection of toxin genes, rDNA sequences and a phylogenetic analysis, fatty acid composition, physiological and biochemical properties, all isolates were identified as P. syringaepv. tabaci. To the best of our knowledge, this is the first report of wildfire induced by P. syringaepv. tabacion mung bean worldwide.

Published
2021
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48. Genetic diversity in Fusarium solani f. sp. pisi based on SSR markers

Author

Xiao Yan-Nong, Wang Xiaoming, Xiang Ni, Zhu Zhendong, and Duan Canxing

Subjects
Genetic diversity, Ecology, biology, Botany, biology.organism_classification, Fusarium solani, Ecology, Evolution, Behavior and Systematics, Nature and Landscape Conservation
Published
2013

49. Identification ofPhoma macdonaldiiBoerema causing black stem of sunflower in China

Author

Wang Xiao-ming, Guo Qing-yuan, Duan Canxing, Xu Biao, and Bai Jianyu

Subjects
biology, Inoculation, Plant Science, Fungus, Pathogenicity, biology.organism_classification, Sunflower, Horticulture, Botany, Helianthus annuus, Phoma, Internal transcribed spacer, Agronomy and Crop Science, Ribosomal DNA
Abstract

A severe disease was observed on sunflower (Helianthus annuus L.) in Ili, Xinjiang China, in 2005. The causal agent was isolated from infected tissues, and its pathogenicity confirmed. Pathogenicity tests showed that the fungus could infect sunflower, which developed the same symptoms under artificial inoculation conditions to those observed in the field. The fungus was identified based on morphological and cultural characteristics as Phoma macdonaldii Boerema. Identifications were confirmed by comparison of the 18S rDNA partial sequence, 28S rDNA partial sequence, and the internal transcribed spacer (ITS) regions 1 and 2, including 5.8S rDNA (ITS1‐5.8S-ITS2). This is the first report of black stem of sunflower caused by P. macdonaldii in China.

Published
2012

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