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4. Simultaneous Electrochemical Determination of Erythromycin and Hemoglobin by Flexible Carbon Cloth Modified with ZnO Nanorod Arrays and Au Nanoparticles by Differential Pulse Voltammetry (DPV).

Author

Zhang, Xiaoyu, Meng, Peiran, Wang, Wei, Li, Xiwen, Yang, Zuan, Du, Shaokai, and Sun, Yue

Subjects
CARBON fibers, GOLD nanoparticles, NANORODS, ERYTHROMYCIN, HEMOGLOBINS
Abstract

Simultaneous determination of erythromycin (Ery) and hemoglobin (Hb) is of great significance for clinical diagnosis of many diseases. ZnO nanorods modified with Au nanoparticles were fabricated as an interface on the flexible carbon cloth (CC) and utilized to simultaneously determine Ery and Hb by a simple electrochemical method. The results demonstrated that Au/ZnO/CC displayed excellent analytical performance for Ery with a low detection limit of 3.38 × 10−7 mg/L and a linear range from 1 × 10−6 to 1 × 10−1 mg/L. At the same time, the sensor also showed a sensitive response to Hb with a low detection limit of 2.07 × 10−7 mg/L and a linear range from 1 × 10−6 to 1 × 10−1 mg/L. This work provides a viable approach for designing a flexible electrode and sensitive simultaneous determination of Ery and Hb. [ABSTRACT FROM AUTHOR]

Published
2024
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6. Fabrication and properties of temperature-responsive imprinted sensors based on fluorescently labeled yeast cells viaMVL ATRPElectronic supplementary information (ESI) available: Schematic of FITC-yeast synthesis (Fig. S1), fluorescence spectra of FITC solutions with different concentrations and the equation of linear regression (Fig. S2), CV curves of different modified electrodes ((a) AuNPs/Au, (b) Ti3C2Tx/AuNPs/Au, and (c) AuNPs/Ti3C2Tx/AuNPs/Au) (Fig. S3), the “on–off” experiment (Fig. S4), comparison of DPV signal responses of different modified electrodes with and without yeast cell solution (Fig. S5), DPV curves of the imprinted polymer modified electrode: blank (a), mixed solution (b) and yeast cell (c) detection using the sensor (Fig. S6), comparison of the linear response range of CIPs/AuNPs/Ti3C2Tx/AuNPs/Au and other materials for the determination of yeast cells (Table S1), and determination in real samples (Table S2). See DOI: https://doi.org/10.1039/d4ay00905c

Author

Chen, Yue, Du, Shaokai, Cui, Ailu, Jiang, Shipeng, He, Yuxuan, Yang, Shuhan, Ma, Ruyi, and Sun, Yue

Abstract

Temperature-responsive yeast cell-imprinted sensors (CIPs/AuNPs/Ti3C2Tx/AuNPs/Au) were prepared based on fluorescein isothiocyanate labeled yeast cells (FITC-yeast) viametal-free visible-light-induced atom transfer radical polymerization (MVL ATRP). Here, N-isopropyl acrylamide (NIPAM) was used as a temperature-responsive functional monomer, α-methacrylic acid (MAA) was chosen as an auxiliary functional monomer, N,N′-methylene bisacrylamide (MBA) was used as a cross-linker, and FITC-yeast was selected as both a template and photocatalyst. Under the optimal conditions, the detection range of the yeast cell-imprinted sensor toward yeast cells was 1.0 × 102to 1.0 × 109cells per mL, and the detection limit was 11 cells per mL (S/N = 3), with a linear equation of ΔI(μA) = 8.44 log[C(cells per mL)] + 7.62 (R2= 0.993). The sensor showed good selective recognition in the presence of interfering substances such as autolyzed yeast cells (AY), dead yeast cells (DY), human mammary epithelial cells (MCF-10A), human breast cancer cells (MCF-7) and Escherichia coli(EC). The sensor also had good consistency and reproducibility. Finally, spiked recovery experiments were performed to investigate the recognition of yeast cells in the actual sample using the yeast cell-imprinted sensor. The spiked recoveries were all in the range of 98.5–108.0%, and the RSD values were all less than 4%, indicating that the sensor had good application prospects.

Published
2024
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