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7 results on '"Drotar, Jesse"'

1. Dynamic Enhancer DNA Methylation as Basis for Transcriptional and Cellular Heterogeneity of ESCs

Author

Massachusetts Institute of Technology. Department of Biology, Song, Yuelin, van den Berg, Patrick R., Markoulaki, Styliani, Soldner, Frank, Dall’Agnese, Alessandra, Henninger, Jonathan E., Drotar, Jesse, Rosenau, Nicholas, Cohen, Malkiel A., Young, Richard A., Semrau, Stefan, Stelzer, Yonatan, Jaenisch, Rudolf, Massachusetts Institute of Technology. Department of Biology, Song, Yuelin, van den Berg, Patrick R., Markoulaki, Styliani, Soldner, Frank, Dall’Agnese, Alessandra, Henninger, Jonathan E., Drotar, Jesse, Rosenau, Nicholas, Cohen, Malkiel A., Young, Richard A., Semrau, Stefan, Stelzer, Yonatan, and Jaenisch, Rudolf

Abstract

Song et al. used an allelic reporter approach to show super-enhancers allelic DNA methylation dynamics underlies locus-specific heterogeneity, which functionally impacts transcription and cellular states of mouse embryonic stem cells., NIH (Grants HD045022, 1U19AI131135, 5R01MH104610, and 1R01GM114864)

Published
2020

2. Dynamic Enhancer DNA Methylation as Basis for Transcriptional and Cellular Heterogeneity of ESCs

Author

Song, Yuelin, primary, van den Berg, Patrick R., additional, Markoulaki, Styliani, additional, Soldner, Frank, additional, Dall’Agnese, Alessandra, additional, Henninger, Jonathan E., additional, Drotar, Jesse, additional, Rosenau, Nicholas, additional, Cohen, Malkiel A., additional, Young, Richard A., additional, Semrau, Stefan, additional, Stelzer, Yonatan, additional, and Jaenisch, Rudolf, additional

Published
2019
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3. Pharmacological enhancement of KCC2 gene expression exerts therapeutic effects on human Rett syndrome neurons and Mecp2 mutant mice

Author

Tang, Xin, primary, Drotar, Jesse, additional, Li, Keji, additional, Clairmont, Cullen D., additional, Brumm, Anna Sophie, additional, Sullins, Austin J., additional, Wu, Hao, additional, Liu, Xiaoxiao Shawn, additional, Wang, Jinhua, additional, Gray, Nathanael S., additional, Sur, Mriganka, additional, and Jaenisch, Rudolf, additional

Published
2019
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4. Molecular Criteria for Defining the Naive Human Pluripotent State

Author

Massachusetts Institute of Technology. Department of Biology, Jaenisch, Rudolf, Theunissen, Thorold W., Friedli, Marc, He, Yupeng, Planet, Evarist, O’Neil, Ryan C., Markoulaki, Styliani, Pontis, Julien, Wang, Haoyi, Iouranova, Alexandra, Imbeault, Michaël, Duc, Julien, Cohen, Malkiel A., Wert, Katherine J., Castanon, Rosa, Zhang, Zhuzhu, Huang, Yanmei, Nery, Joseph R., Drotar, Jesse, Lungjangwa, Tenzin, Trono, Didier, Ecker, Joseph R., Massachusetts Institute of Technology. Department of Biology, Jaenisch, Rudolf, Theunissen, Thorold W., Friedli, Marc, He, Yupeng, Planet, Evarist, O’Neil, Ryan C., Markoulaki, Styliani, Pontis, Julien, Wang, Haoyi, Iouranova, Alexandra, Imbeault, Michaël, Duc, Julien, Cohen, Malkiel A., Wert, Katherine J., Castanon, Rosa, Zhang, Zhuzhu, Huang, Yanmei, Nery, Joseph R., Drotar, Jesse, Lungjangwa, Tenzin, Trono, Didier, and Ecker, Joseph R.

Abstract

Recent studies have aimed to convert cultured human pluripotent cells to a naive state, but it remains unclear to what extent the resulting cells recapitulate in vivo naive pluripotency. Here we propose a set of molecular criteria for evaluating the naive human pluripotent state by comparing it to the human embryo. We show that transcription of transposable elements provides a sensitive measure of the concordance between pluripotent stem cells and early human development. We also show that induction of the naive state is accompanied by genome-wide DNA hypomethylation, which is reversible except at imprinted genes, and that the X chromosome status resembles that of the human preimplantation embryo. However, we did not see efficient incorporation of naive human cells into mouse embryos. Overall, the different naive conditions we tested showed varied relationships to human embryonic states based on molecular criteria, providing a backdrop for future analysis of naive human pluripotency., Simons Foundation Autism Research Initiative (SFLIFE #286977), United States. National Institutes of Health (RO1-CA084198)

Published
2017

5. Molecular Criteria for Defining the Naive Human Pluripotent State

Author

Theunissen, Thorold W., primary, Friedli, Marc, additional, He, Yupeng, additional, Planet, Evarist, additional, O’Neil, Ryan C., additional, Markoulaki, Styliani, additional, Pontis, Julien, additional, Wang, Haoyi, additional, Iouranova, Alexandra, additional, Imbeault, Michaël, additional, Duc, Julien, additional, Cohen, Malkiel A., additional, Wert, Katherine J., additional, Castanon, Rosa, additional, Zhang, Zhuzhu, additional, Huang, Yanmei, additional, Nery, Joseph R., additional, Drotar, Jesse, additional, Lungjangwa, Tenzin, additional, Trono, Didier, additional, Ecker, Joseph R., additional, and Jaenisch, Rudolf, additional

Published
2016
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6. Molecular Criteria for Defining the Naive Human Pluripotent State

Author

Theunissen, Thorold W, Friedli, Marc, He, Yupeng, Planet, Evarist, O'Neil, Ryan C, Markoulaki, Styliani, Pontis, Julien, Wang, Haoyi, Iouranova, Alexandra, Imbeault, Michaël, Duc, Julien, Cohen, Malkiel A, Wert, Katherine J, Castanon, Rosa, Zhang, Zhuzhu, Huang, Yanmei, Nery, Joseph R, Drotar, Jesse, Lungjangwa, Tenzin, Trono, Didier, Ecker, Joseph R, Jaenisch, Rudolf, Massachusetts Institute of Technology. Department of Biology, Jaenisch, Rudolf, Imbeault, Michael [0000-0002-0073-0922], and Apollo - University of Cambridge Repository

Subjects
Male, Pluripotent Stem Cells, X chromosome reactivation, Transcription, Genetic, Cleavage Stage, Ovum, Human Embryonic Stem Cells, DNA, Mitochondrial, Morula, Polymerase Chain Reaction, Cell Line, Genomic Imprinting, Mice, Genetics, Animals, Humans, Chromosomes, Human, X, DNA methylation, Chimera, Genome, Human, Gene Expression Profiling, Cell Differentiation, Cell Biology, embryonic stem cells, pluripotency, Mitochondria, Blastocyst, mouse-human chimeras, DNA Transposable Elements, Molecular Medicine, Female, transposable elements, imprinting
Abstract

Recent studies have aimed to convert cultured human pluripotent cells to a naive state, but it remains unclear to what extent the resulting cells recapitulate in vivo naive pluripotency. Here we propose a set of molecular criteria for evaluating the naive human pluripotent state by comparing it to the human embryo. We show that transcription of transposable elements provides a sensitive measure of the concordance between pluripotent stem cells and early human development. We also show that induction of the naive state is accompanied by genome-wide DNA hypomethylation, which is reversible except at imprinted genes, and that the X chromosome status resembles that of the human preimplantation embryo. However, we did not see efficient incorporation of naive human cells into mouse embryos. Overall, the different naive conditions we tested showed varied relationships to human embryonic states based on molecular criteria, providing a backdrop for future analysis of naive human pluripotency., Simons Foundation Autism Research Initiative (SFLIFE #286977), United States. National Institutes of Health (RO1-CA084198)

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7. Pharmacological enhancement of KCC2gene expression exerts therapeutic effects on human Rett syndrome neurons and Mecp2mutant mice

Author

Tang, Xin, Drotar, Jesse, Li, Keji, Clairmont, Cullen D., Brumm, Anna Sophie, Sullins, Austin J., Wu, Hao, Liu, Xiaoxiao Shawn, Wang, Jinhua, Gray, Nathanael S., Sur, Mriganka, and Jaenisch, Rudolf

Abstract

KCC2 expression–enhancing small molecules rescue cellular and behavior deficits in human RTT neurons and Mecp2mutant mice.

Published
2019
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