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The ability of headwater bed and suspended sediments to mitigate non-point agricultural phosphorus (P) loads to the lower Great Lakes is recognized, but the specific biogeochemical processes promoting sediment P retention or internal P release remain poorly understood. To elucidate these mechanisms, three headwater segments located within priority watersheds of Southern Ontario, Canada, were sampled through the growing season of 2018-2020. The study employed equilibrium P assays along with novel assessments of legacy watershed nutrients, nitrogen (N) concentrations, sediment redox, and microbial community composition. 20-year data revealed elevated total P (TP) and total Nitrogen (TN) at an inorganic fertilizer and manure fertilizer-impacted site, respectively. Overall, sampled sites acted as P sinks; however, agricultural sediments exhibited significantly lower buffering capacity compared to a reference forested watershed. Collection of fine suspended sediment (<63 µm) through time-integrated sampling showed the suspended load at the inorganic-fertilized site was saturated with P, indicating a greater potential for P release into surface waters compared to bed sediments. Through vertical microsensor profiling and DNA sequencing of the sediment microbial community, site-specific factors associated with a distinct P-source event were identified. These included rapid depletion of dissolved oxygen (DO) across the sediment water interface (SWI), as well as the presence of nitrate-reducing bacterial and ammonia-oxidizing archaeal (AOA) genera. This research provides valuable insights into the dynamics of P in headwaters, shedding light on P retention and release. Understanding these processes is crucial for effective management strategies aimed at mitigating P pollution to the lower Great Lakes., (© 2023. The Author(s).)

Microbial assessments of recreational water have traditionally focused on culturing or DNA-based approaches of the planktonic water column, omitting influence from microbe-sediment relationships. Sediment (bed and suspended) has been shown to often harbour levels of bacteria higher than the planktonic phase. The fate of suspended sediment (SS) bacteria is extensively related to transport dynamics (e.g., deposition) of the associated sediment/floc. When hydraulic energy allows, SS will settle, introducing new (potentially pathogenic) organisms to the bed. With turbulence, including waves, currents and swimmers, the risk of human ingestion is elevated due to resuspension of bed sediment and associated microbes. This research used multiplex nanofluidic reverse transcriptase quantitative PCR on RNA of bacteria associated with bed and SS to explore the active bacteria in freshwater shorelines. Bacterial genes of human health concern regarding recreational water use were targeted, such as faecal indicator bacteria (FIB), microbial source tracking genes and virulence factors from waterborne pathogens. Results indicate avian sources (i.e., gulls, geese) to be the largest nonpoint source of FIB associated with sediment in Great Lakes shorelines. This research introduces a novel approach to microbial water quality assessments and enhances our understanding of microbe-sediment dynamics and the quality of freshwater beaches., (© 2023 The Authors. Environmental Microbiology published by Applied Microbiology International and John Wiley & Sons Ltd.)

The introduction and proliferation of pathogenic organisms in aquatic systems is a serious global issue that consequently leads to economic, financial, and health concerns. Health and safety related to recreational water use is typically monitored through water quality assessments that are outdated and can be misleading. These traditional methods focus on broad taxa groups, provide no insight into the active community or source of contamination, and the sediment compartments (bed and suspended) are often overlooked. To bridge this knowledge gap, our study aimed to 1) examine the metatranscriptome of the microbial community associated with suspended sediment (SS) in freshwater systems; 2) explore the influence of SS in tributaries to the littoral zone of the receiving lake; and 3) compare the SS fraction with previously reported nearshore bed sediment data. Samples were collected seasonally from Lake St. Clair and Lake Erie. Beaches in this region are influenced by both agriculture runoff and continued urban expansion. Results show that both adjacent tributary and beach SS have similar microbial functional diversity and are strongly correlated by site and season. We identified expression of transcripts encoding sequences with similarities to genes involved in nine bacterial infectious disease pathways, including legionellosis (sdhA) and Vibrio cholerae pathogenesis. According to MG-RAST gene categories, lake samples typically showed higher overall expression (p < 0.05) of transcripts with similarities to genes involved in infectious disease pathways compared to the tributaries, with summer upregulated (p < 0.05) compared to fall. Our data suggests SS acts as a strong vector for pathogen transport, making this facet an important area for further research as it pertains to human health regarding recreational water use. To our knowledge, this work is the first to investigate SS in aquatic microbial communities using metatranscriptomic analyses and has significant potential to help address growing issues of microbial contamination impacting freshwater security., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Microbial communities are an important aspect of overall riverine ecology; however, appreciation of the effects of anthropogenic activities on unique riverine microbial niches, and how the collection of these samples affects the observed diversity and community profile is lacking. We analyzed prokaryotic and eukaryotic communities from surface water, biofilms, and suspended load niches along a gradient of oil sands-related contamination in the Athabasca River (Alberta, Canada), with suspended load or particle-associated communities collected either via Kenney Sampler or centrifugation manifold. At the phylum level, different niche communities were highly similar to each other and across locations. However, there were significant differences in the abundance of specific genera among the different niches and across sampling locations. A generalized linear model revealed that use of the Kenney Sampler resulted in more diverse bacterial and eukaryotic suspended load community than centrifugal collection, though suspended load communities collected by any means remained stably diverse across locations. Although there was an influence of water quality parameters on community composition, all sampled sites support diverse bacterial and eukaryotic communities regardless of the degree of contamination, highlighting the need to look beyond ecological diversity as a means of assessing ecological perturbations, and consider collecting samples from multiple niche environments.

Natural sediment flocs are fragile, highly irregular, loosely bound aggregates comprising minerogenic and organic material. They contribute a major component of suspended sediment load and are critical for the fate and flux of sediment, carbon and pollutants in aquatic environments. Understanding their behaviour is essential to the sustainable management of waterways, fisheries and marine industries. For several decades, modelling approaches have utilised fractal mathematics and observations of two dimensional (2D) floc size distributions to infer levels of aggregation and predict their behaviour. Whilst this is a computationally simple solution, it is highly unlikely to reflect the complexity of natural sediment flocs and current models predicting fine sediment hydrodynamics are not efficient. Here, we show how new observations of fragile floc structures in three dimensions (3D) demonstrate unequivocally that natural flocs are non-fractal. We propose that floc hierarchy is based on observations of 3D structure and function rather than 2D size distribution. In contrast to fractal theory, our data indicate that flocs possess characteristics of emergent systems including non-linearity and scale-dependent feedbacks. These concepts and new data to quantify floc structures offer the opportunity to explore new emergence-based floc frameworks which better represent natural floc behaviour and could advance our predictive capacity.

Pathogenic bacteria associated with freshwater ecosystems can pose significant health risks particularly where recreational water use is popular. Common water quality assessments involve quantifying indicator Escherichia coli within the water column but neglect to consider physical and geochemical factors and contributions from the sediment. In this study, we used high-throughput sequencing to investigate sediment microbial communities at four freshwater public beaches in southern Ontario, Canada and analysed community structure, function, and gene expression with relation to geographical characteristics. Our results indicate that beach sediments at the sediment-water interface could serve as potential sources of bacterial contamination under low-energy environments with tightly packed small sediment particles compared with high-energy environments. Further, the absence of pathogens but expression of pathogenic transcripts suggests occurrence of alternate gene acquisition. Pathogenicity at these locations included expression of Salmonella virulence factors, genes involved in pertussis, and antimicrobial resistance. Finally, we introduce a proposed universal bacterial pathogen model to consider the combined and synergistic processes used by these microbes. To our knowledge, this is the first study of its kind to investigate chemolithotrophic activity related to pathogens within bed sediment at freshwater beaches. This work helps advance current understanding of health risks in these environments., (© 2019 Society for Applied Microbiology and John Wiley & Sons Ltd.)

Within all aquatic environments, aside from the physical dispersal of dissolved and/or particulate phase contaminants, alteration from both biological and chemical processes are shown to change the chemistry of the parent compounds. Often these alterations can lead to secondary influences because of cooperative microbial processes (i.e. coupled respiratory pathways and/or energy and biodegradation cycles), complicating our understanding of the biological impact that these mobile compounds impose on ecosystem health. The McMurray Formation (MF) (the formation constituting the minable bituminous oil sands) is a natural, ongoing source of hydrocarbon-bound sediments to river ecosystems in the region (via terrestrial and aquatic erosion), providing a natural "mesocosm" to track and characterize the effects of these compounds on regional aquatic primary productivity. Here we characterize the natural, in-situ microbial response to increasing hydrocarbon exposure along a river continuum in the downstream direction. Using the Steepbank River (STB), suspended and bed sediment samples were collected at 3 sites from upstream to downstream, as the water flows into and through the MF. Samples were then analyzed for the active, in-situ gene expression of the microbial communities. Results from both suspended and bed sediments show clear and significant shifts in the microbial metabolic processes within each respective compartment, in response to the elevated polycyclic aromatic compound (PAC) concentrations. Specific genes likely responsible for hydrocarbon breakdown (Alkane Monooxygenase, Benzoyl-CoA Reductase etc.) experience elevated expression levels, while certain energy metabolism genes (nitrogen, sulfur, methane) reveal fundamental shifts in their pathway specificity, indicating an adaptation response in their basic energy metabolism. Expression from suspended sediments reveal subtle yet delayed metabolic response further downstream compared to bed sediments, indicative of the erosion and transport dynamics within a lotic system. These results provide insight into the use of novel clusters of gene biomarkers to track the active, in-situ microbial response of both emerging and legacy contaminants. Such information will be important in determining the best management strategies for the monitoring and assessment of aquatic health in both natural and contaminated ecosystems., (Crown Copyright © 2019. Published by Elsevier Ltd. All rights reserved.)

This paper investigated the sediment/contaminant continuum of bitumen containing sediment, from eroded exposed natural bitumen outcrops to river depositional zone, in order to improve our understanding of the transitional sediment, chemistry and toxicological influence on aquatic health. To achieve this aim, we linked a rainfall simulator with an annular flume to allow for connectivity between terrestrial erosion to stream flow. Bulk sediments were collected from the minable McMurray Formation (MF) on the Ells (EL) and Steepbank (STB) Rivers and from the Clearwater Formation (CF) on the STB. All plots generated similar washoff rates (~25 L hr -1 ) and fine eroded sediment (d 50  = 5 μm) regardless of strata. The CF generated the highest total eroded sediment (TES) followed by EL-MF and STB-MF. The STB-MF generated up to three orders of magnitude lower TES than the STB-CF. The EL generated the highest PAH concentrations, however, when normalizing by TES mass delivered to the flume, STB-MF PAH was one to two orders of magnitude higher per unit mass than EL-MF. The TES concentrations were inversely proportional to the degree of bitumen within the sediment (STB-MF > EL-MF > STB-CF). Once the TES was suspended in the flume, there was a change in the dominant PAH for most classes [e.g., Dibenzothiophenes C4 (parent material) to a C3 (TES)]. Further, no flocculation and the buoyant properties of the TES suggested that the sediment and associated PAHs may travel long distances. The EL-MF proved to be the most toxic to fathead minnow embryo survival due principally to the high PAH concentrations and sediment loads. The CF exhibited no toxicological effect. This work has shown the importance of assessing sediment/contaminant characteristics over the continuum (terrestrial erosion/washoff to river deposition) in order to support basin wide management strategies for the protection of aquatic health., (Crown Copyright © 2019. Published by Elsevier B.V. All rights reserved.)

The success and sustainability of aquatic ecosystems are driven by the complex, cooperative metabolism of microbes. Ecological engineering strategies often strive to harness this syntrophic synergy of microbial metabolism for the reclamation of contaminated environments worldwide. Currently, there is a significant knowledge gap in our understanding of how the natural microbial ecology overcomes thermodynamic limitations in recovering contaminated environments. Here, we used in-situ metatranscriptomics and associated metataxonomic analyses on sediments collected from naturalized freshwater man-made reservoirs within the Athabasca Oil Sands region of Alberta, Canada. These reservoirs are unique since they are untouched by industrial mining processes and serve as representative endpoints for model landscape reconstruction. Results indicate that a microbial syntrophic cooperation has been established represented by the oxygenic and anoxygenic phototrophs, sustained through the efficient use of novel cellular mechanistic adaptations tailored to these unique thermodynamic conditions. Specifically, chemotaxis transcripts (cheY & MCPs-methyl-accepting chemotaxis proteins) were highly expressed, suggesting a highly active microbial response to gradients in environmental stimuli, resulting indirectly from hydrocarbon compound alteration. A high expression of photosynthetic activity, likely sustaining nutrient delivery to the similarly highly expressed methanogenic community acting in syntrophy during the breakdown of organics. Overall the more diversified functionality within sub-oxic sample locations indicates an ability to maintain efficient metabolism under thermodynamic constraints. This is the first study to holistically identify and characterize these types of in-situ, metabolic processes and address their thermodynamic feasibility within a global context for large landscape reconstruction. These characterizations of regional, natural landscapes surrounding the oil sands mining operation are severely lacking, but truly provide invaluable insight into end-point goals and targets for reclamation procedures., (Copyright © 2019 Elsevier B.V. All rights reserved.)

Within the Oil-Sands industry in Alberta, Canada, tailings ponds are used as water recycling and tailings storage facilities (TSF) for mining activities. However, there could be possible circumstances under which a sudden breach of an embankment confining one of the TSFs may occur. Such a tailings pond breach would result in a sudden release of a huge volume of Oil Sands process-affected water (OSPW) and sediment slurry containing substantial amount of chemical constituents that would follow the downstream drainage paths and subsequently enter into the Lower Athabasca River (LAR). This study investigates the implications of OS tailings release on the water and sediment quality of the LAR by simulating the fate of sediment and associated chemicals corresponding to a hypothetical breach and release scenarios from a select set of tailings ponds using a two-dimensional hydrodynamic and constituent transport model. After predicting the total volume, time evolution and concentration of sediment and associated chemicals (metals, polycyclic aromatic hydrocarbons (PAHs) and naphthenic acids (NAs)) reaching the LAR, the transport and deposition of these materials within the study reach is simulated. The results show that, depending on tailings release locations, between 40 and 70% of the sediment and associated chemicals get deposited onto the river bed of the 160 km study reach while the rest leaves the study domain during the first three days following the release event. These sediment/chemicals deposited during the initial spill may also have long-term effects on the water quality and aquatic ecosystem of the river and the downstream delta. However, care has to be taken in interpreting the results as further analysis has shown that the outcomes of such model simulations are very sensitive to the various underlying assumptions as well as the values assigned to some model parameters representing the physical properties of the tailings material., (Crown Copyright © 2018. Published by Elsevier B.V. All rights reserved.)

Bitumen-bearing suspended sediment (SS) eroded from the McMurray Formation (MF) are fine grained (silts and clays) and coated with natural hydrophobic oils. This results in poor settling and long range transport of associated contaminants. There was a longitudinal increase in polycyclic aromatic compound (PAC) concentrations for rivers that erode the MF from upstream to downstream regardless of time-of-year, while loads were substantially increased during high flow periods when the erosive forces are the greatest and the overland flow contribution is high. Within the MF, variation in PAC contributions is seen by the Ells River having higher loads than the Steepbank River. Using the Ells and Steepbank as examples, double plot PACs ratios suggest that the PAC concentrations and signatures vary spatially within the MF but that the weathering processes may be the same. Plots of the various homologs of PACs generally illustrated a normal distribution which suggests petrogenic origins. However, several PAC ratios suggested that both the parent material and the SS are pyrogenic in nature. While it is likely that some level of atmospheric deposition (anthropogenic or from forest fire) is incorporated into the SS of the rivers, it is likely to be limited relative to the proportion of naturally eroded MF sediments. Additional analysis will be needed to distinguish the relative risk of both anthropogenic (e.g., industrial operations) and natural sources (bitumen deposits, forest fire) of PACs to the SS and to long-range depositional environments, as they may have potential aquatic ecological effects., (Crown Copyright © 2018. Published by Elsevier B.V. All rights reserved.)

Baseline biogeochemical surveys of natural environments is an often overlooked field of environmental studies. Too often research begins once contamination has occurred, with a knowledge gap as to how the affected area behaved prior to outside (often anthropogenic) influences. These baseline characterizations can provide insight into proposed bioremediation strategies crucial in cleaning up chemical spill sites or heavily mined regions. Hence, this study was conducted to survey the in-situ microbial activity within freshwater hydrocarbon-rich environments cutting through the McMurray formation - the geologic strata constituting the oil sands. We are the first to report in-situ functional variations among these freshwater microbial ecosystems using metatranscriptomics, providing insight into the in-situ gene expression within these naturally hydrocarbon-rich sites. Key genes involved in energy metabolism (nitrogen, sulfur and methane) and hydrocarbon degradation, including transcripts relating to the observed expression of methane oxidation are reported. This information provides better linkages between hydrocarbon impacted environments, closing knowledge gaps for optimizing not only oil sands mine reclamation but also enhancing microbial reclamation strategies in various freshwater environments. These finding can also be applied to existing contaminated environments, in need of efficient reclamation efforts., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

Within the oil sands industry, tailings ponds are used as a means of retaining tailings until a reclamation technology such as end pit lakes (EPLs) can be developed and optimized to remediate such tailings with a water cap (although dry-land strategies for tailing reclamation are also being developed). EPLs have proven successful for other mining ventures (e.g. metal rock mines) in eventually mitigating contaminant loads to receiving waters once biochemical remediation has taken place (although the duration for this to occur may be decades). While the biological interactions at the sediment water interface of tailings ponds or EPLs have been shown to control biogeochemical processes (i.e. chemical fluxes and redox profiles), these have often been limited to static microcosm conditions. Results from such experiments may not tell the whole story given that the sediment water interface often represents a dynamic environment where erosion and deposition may be occurring in association with microbial growth and decay. Mobilization of sediments and associated contaminants may therefore have a profound effect on remediation rates and, as such, may decrease the effectiveness of EPLs as viable reclamation strategies for mining industries. Using a novel core erosion system (U-GEMS), this paper examines how the microbial community can influence sediment water interface stability and how the biofilm community may change with tailings age and after disturbance (biofilm reestablishment). Shear strength, eroded mass measurements, density gradients, high-resolution microscopy, and microbial community analyses were made on 2 different aged tailings (fresh and ∼38 years) under biotic and abiotic conditions. The same experiments were repeated as duplicates with both sets of experiments having consolidation/biostabilization periods of 21 days. Results suggest that the stability of the tailings varies between types and conditions with the fresh biotic tailings experiencing up to 75% more biostabilization than the same abiotic tailings. Further, greater microbial diversity in the aged pond could be a contributing factor to the overall increase in stability of this material over the fresh tailings source., (Copyright © 2016 Elsevier Ltd. All rights reserved.)

The erosion, transport and fate of sediments and associated contaminants are known to be influenced by both particle characteristics and the flow dynamics imparted onto the sediment. The influential role of bitumen containing hydrophobic sediments and the microbial community on sediment dynamics are however less understood. This study links an experimental evaluation of sediment erosion with measured sediment-associated contaminant concentrations and microbial community analysis to provide an estimate of the potential for sediment to control the erosion, transport and fate of contaminants. Specifically the paper addresses the unique behaviour of hydrophobic sediments and the role that the microbial community associated with hydrophobic sediment may play in the transport of contaminated sediment. Results demonstrate that the hydrophobic cohesive sediment demonstrates unique transport and particle characteristics (poor settling and small floc size). Biofilms were observed to increase with consolidation/biostabilization times and generated a unique microbial consortium relative to the eroded flocs. Natural oil associated with the flocs appeared to be preferentially associated with microbial derived extracellular polymeric substances. While PAHs and naphthenic acid increased with increasing shear (indicative of increasing loads), they tended to decrease with consolidation/biostabilization (CB) time at similar shears suggesting a chemical and/or biological degradation. PAH and napthenic acid degrading microbes decreased with time as well, which may suggest that there was a reduced pool of PAHs and naphthenic acids available resulting in their die off. This study emphasizes the importance that any management strategies and operational assessments for the protection of human and aquatic health incorporate the sediment (suspended and bed sediment) and biological (biofilm) compartments and the energy dynamics within the system in order to better predict contaminant transport., (Crown Copyright © 2016. Published by Elsevier Ltd. All rights reserved.)

The dynamic interaction of bacteria within bed sediment and suspended sediment (i.e., floc) in a wave-dominated beach environment was assessed using a laboratory wave flume. The influence of shear stress (wave energy) on bacterial concentrations and on the partitioning and transport of unattached and floc-associated bacteria was investigated. The study showed that increasing wave energy (0.60 and 5.35 N/s) resulted in a 0.5 to 1.5 log increase in unattached cells of the test bacterium Pseudomonas sp. strain CTO7::gfp-2 in the water column. There was a positive correlation between the bacterial concentrations in water and the total suspended solids, with the latter increasing from values of near 0 to up to 200 mg/L over the same wave energy increase. The median equivalent spherical diameter of flocs in suspension also increased by an order of magnitude in all experimental trials. Under both low (0.60 N/s) and high (5.35 N/s) energy regime, bacteria were shown to preferentially associate with flocs upon cessation of wave activity. The results suggest that collecting water samples during periods of low wave action for the purpose of monitoring the microbiological quality of water may underestimate bacterial concentrations partly because of an inability to account for the effect of shear stress on the erosion and mobilization of bacteria from bed sediment to the water column. This highlights the need to develop a more comprehensive beach analysis strategy that not only addresses presently uncharacterized shores and sediments but also recognizes the importance of eroded flocs as a vector for the transport of bacteria in aquatic environments.

Activated sludge flocs that are carried to the final effluent can significantly decrease the effectiveness of ultraviolet (UV) disinfection of wastewater. This effect is detected in a typical UV dose-response curve, where at higher UV doses there is a decrease in the inactivation rate (tailing). In this study, the effect of activated sludge process conditions on the UV inactivation kinetics of flocs was investigated. The conditions compared were nitrifying vs. non-nitrifying vs. an enhanced biological nutrient removal-University of Cape Town (BNR-UCT) system. The results showed that the flocs generated in the BNR-UCT process were easier to disinfect. The final effluent from the BNR-UCT process also showed improved kinetics of inactivation and reached higher levels of disinfection. The nitrifying system's final effluent had a lower number of initial fecal coliforms, which contributed to reaching higher disinfection levels compared to the non-nitrifying system.

Clinically important antibiotic resistance genes were detected in culturable bacteria and class 1 integron gene cassettes recovered from suspended floc, a significant aquatic repository for microorganisms and trace elements, across freshwater systems variably impacted by anthropogenic activities. Antibiotic resistance gene cassettes in floc total community DNA differed appreciably in number and type from genes detected in bacteria cultured from floc. The number of floc antibiotic resistance gene cassette types detected across sites was positively correlated with total (the sum of Ag, As, Cu, and Pb) trace element concentrations in aqueous solution and in a component of floc readily accessible to bacteria. In particular, concentrations of Cu and Pb in the floc component were positively correlated with floc resistance gene cassette diversity. Collectively, these results identify suspended floc as an important reservoir, distinct from bulk water and bed sediment, for antibiotic resistance in aquatic environments ranging from heavily impacted urban sites to remote areas of nature reserves and indicate that trace elements, particularly Cu and Pb, are geochemical markers of resistance diversity in this environmental reservoir. The increase in contamination of global water supplies suggests that aquatic environments will become an even more important reservoir of clinically important antibiotic resistance in the future.

Hydrodynamic forces generated by an orifice plate under low pressure were examined as a means of disrupting flocs, in order to improve disinfection of treated wastewater effluents. Changes in cavitation conditions were found to have little impact on the extent of particle breakage in this experimental setup. The rate of strain (flow rate divided by the hole radius cubed), however, was found to be the best predictor of floc breakage. Floc breakage was not affected by changes in floc concentration, but was very sensitive to differences between flocs collected from different sources. Larger flocs (90 to 106 microm) were broken apart to a greater extent than smaller ones (53 to 63 microm). Hydrodynamic treatment decreased the viability of bacteria associated with large flocs, and also increased the ultraviolet dose response by up to one log unit (i.e., a factor of ten). Subjecting final effluent wastewaters to hydrodynamic treatment, therefore, provides a treatment strategy for conditions in which the presence of flocs limits the level of disinfection that can be achieved.

Significantly higher concentrations of Ag, As, Cu, Ni and Co are found in floc compared to bed sediments across six variably impacted aquatic ecosystems. In contrast to the observed element and site-specific bed sediment trace element (TE) partitioning patterns, floc TE sequestration is consistently dominated by amorphous oxyhydroxides (FeOOH), which account for 30-79% of floc total TE concentrations, irrespective of system physico-chemistry or elements involved. FeOOH consistently occur in significantly higher concentrations in floc than within bed sediments. Further, comparative concentration factors indicate significantly higher TE reactivity of floc-FeOOH relative to sediment-FeOOH in all systems investigated, indicating that both the greater abundance and higher reactivity of floc-FeOOH contribute to enhanced floc TE uptake. Results indicate that floc-organics (live cells and exopolymeric substances, EPS) directly predict floc-FeOOH concentrations, suggesting an organic structural role in the collection/templating of FeOOH. This, in turn, facilitates the sequestration of TEs associated with floc-FeOOH formation, imparting the conserved FeOOH "signature" on floc TE geochemistry across sites. Results demonstrate that the organic rich nature of floc exerts an important control over TE geochemistry in aquatic environments, ultimately creating a distinct solid with differing controls over TE behavior than bed sediments in close proximity (<0.5 m).

Runoff from agricultural watersheds can carry a number of agricultural pollutants and pathogens; often associated with the sediment fraction. Deposition of this sediment can impact water quality and the ecology of the river, and the re-suspension of such sediment can become sources of contamination for reaches downstream. In this paper a modelling framework to predict sediment and associated microbial erosion, transport and deposition is proposed for the South Nation River, Ontario, Canada. The modelling framework is based on empirical relationships (deposition and re-suspension fluxes), derived from laboratory experiments in a rotating circular flume using sediment collected from the river bed. The bed shear stress governing the deposition and re-suspension processes in the stream was predicted using a one dimensional mobile boundary flow model called MOBED. Counts of live bacteria associated with the suspended and bed sediments were used in conjunction with measured suspended sediment concentration at an upstream section to allow for the estimation of sediment associated microbial erosion, transport and deposition within the modelled river reach. Results suggest that the South Nation River is dominated by deposition periods with erosion only occurring at flows above approximately 250 m(3) s(-1) (above this threshold, all sediment (suspended and eroded) with associated bacteria are transported through the modelled reach). As microbes are often associated with sediments, and can survive for extended periods of time, the river bed is shown to be a possible source of pathogenic organisms for erosion and transport downstream during large storm events. It is clear that, shear levels, bacteria concentrations and suspended sediment are interrelated requiring that these parameters be studied together in order to understand aquatic microbial dynamics. It is important that any management strategies and operational assessments for the protection of human and aquatic health incorporate the sediment compartments (suspended and bed sediment) and the energy dynamics within the system in order to better predict the concentration of indicator organism., (Crown Copyright © 2011. Published by Elsevier Ltd. All rights reserved.)

Urban storm water run off can reduce the quality of receiving waters due to high sediment load and associated sediment-bound contaminants. Consequently, urban water management systems, such as detention ponds, that both modify water quantity through storage and improve water quality through sediment retention are frequently-used best management practices. To manage such systems effectively and to improve their efficiency, there is a need to understand the dynamics (transport and settling) of sediment, and in particular the fine sediment fraction (<63 μm) and its associated contaminants within urban storm water management systems. This can be difficult to achieve, as modelling the transport behaviour of fine-grained and cohesive sediment is problematic and field-based measurements can be costly, time-consuming and unrepresentative. The aim of this study was to test the application of a novel cohesive sediment tracer and to determine fine sediment transport dynamics within a storm water detention pond. The cohesive sediment tracer used was a holmium labelled montmorillonite clay which flocculated and had similar size and settling velocity to the natural pond sediment it was intended to mimic. The tracer demonstrated that fine sediment was deposited across the entire pond, with the presence of reed beds and water depth being important factors for maximising sediment retention. The results of the sediment tracer experiment were in good agreement with those of a mathematical sediment transport model. Here, the deposited sediment tracer was sampled by collecting and analysing surface pond sediments for holmium. However, analysis and sampling of the three dimensional suspended tracer 'cloud' may provide more accurate information regarding internal pond sediment dynamics., (Copyright © 2011 Elsevier Ltd. All rights reserved.)

Significantly higher concentrations of Ag, As, Cu, Co, Ni, and Pb are found in suspended floc compared to surficial bed sediments for a freshwater beach in Lake Ontario. Contrasting observed element-specific bed sediment metal partitioning patterns, floc sequestration for all elements is dominated by one substrate: amorphous oxyhydroxides. More specifically, floc metal scavenging is controlled by floc biogeochemical architecture. Floc organics, largely living microbial cells and associated exopolymeric substances (EPS), act as scaffolds for the collection and/or templating of amorphous Fe oxyhydroxides. While interactions between floc organics and amorphous Fe oxyhydroxides affected floc sorption behavior, specific element affinities and competition for these limited substrates was important for overall floc partitioning. Further, assessment of metal dynamics during stormy conditions indicated energy-regime driven shifts in floc and bed sediment partitioning that were specifically linked to the exchange of floc and bed sedimentary materials. These novel results demonstrate that the microbial nature of floc formation exerts an important control on floc metal dynamics distinguishable from surficial bed sediments and that hydrologic energy-regime is an important factor to consider in overall floc metal behavior, especially in beach environments.

The erosion characteristics and bed stability of wildfire-affected stream sediment were measured in an annular flume. Biofilms were grown in the flume on cohesive streambed sediments collected from a wildfire affected stream and a reference undisturbed stream in southern Alberta, Canada. Examined factors that influence sediment erosion, settling and bed stability included applied shear stress, geochemical and physical properties of the sediment, floc structural characteristics and consolidation period (2, 7, 14 days). Erosion characteristics and sediment properties were strongly influenced by wildfire, consolidation period and bed biostabilization. The fire-modified sediment was more resistant to erosion than the reference unburned sediment. Settling velocities were lower in the burned sediment due to higher organic content and porosity. The critical shear stresses for erosion were 1.6 and 1.8 times higher for the burn-associated sediment after 7 and 14 days of consolidation. The differences are related to the greater degree and spatial extent (depth) of biofilm attachment in the burned sediment. Erosion depths were 4-8 times higher in burned sediment as a result of wildfire-associated biostabilization., (Copyright © 2010 Elsevier Ltd. All rights reserved.)

The objective of this research is to explore the fundamental characteristics of how particles in wastewater respond to ultrasound, with an aim to improve wastewater disinfection. Particles of a predetermined size fraction and concentration were treated with varying doses of ultrasound at 20.3 kHz. Ultrasonic power transfer to the fluid was measured using calorimetry or acoustical measurements. Image analysis particle counting was used to measure the size distribution of particles before and after ultrasound treatment. The influence of three parameters: particle origin (raw wastewater or from the aeration basin of the activated sludge process), particle concentration, and particle size on the percentage of particle breakage after ultrasound treatment was compared. It was found that raw wastewater and aeration basin particles of the same size fraction (90-106 microm) responded to ultrasound in a similar way. Particle breakage was not affected by changes in particle concentration from 100 to 400 particles per mL. Larger wastewater particles (90-250 microm) were more susceptible to breakage than smaller ones (38-63 microm diameter). The percentage of particle breakage increased linearly with a logarithmic increase in the ultrasound energy density, that is the ultrasound energy delivered per unit volume of the sample (R(2)=0.48-0.91). An expression that predicts the percent of particles broken as a function of ultrasound energy density is provided.

The transport and fate of indicator E. coli and Salmonella are shown to be highly influenced by their relationship with flocculated suspended and bed sediment particles. Flocs were found to dominate the suspended sediment load and have the effect of increasing the downward flux of the sediment to the river bed. Bacteria counts were consistently higher within sediment compartments (suspended and bed) than for the water alone, with the bed sediment found to represent a possible reservoir of pathogens for subsequent remobilization and transport to potentially high risk areas. The mechanism of microbial attachment and entrapment within the sediment was strongly linked to the EPS fibrils secreted by the biological consortium of the aquatic system. It is suggested that the sediment/pathogen relationship should be of concern to public health officials because of its potential effects on pathogen source fate and effect with implications on public health risk assessment. Current standard sampling strategies, however, are based on an assumption that bacteria are entirely planktonic and do not account for the potentially significant concentration of bacteria from the sediment compartments. The lack of understanding around pathogen/sediment associations may lead to an inaccurate estimate of public health risk, and, as such, possible modification of sampling strategies to reflect this association may be warranted.

Variations in concentrations of polycyclic aromatic hydrocarbons (PAHs) and microbial community indicators were investigated in representative highly contaminated and less contaminated surface sediment sites of Hamilton Harbour. Inputs of PAH to the upper 3cm of sediments up to four times the average upper sediment concentrations were observed. Associated PAH fingerprint profiles indicated that the source was consistent with the PAH source to the industrial region of the harbour. Increased PAH loadings were associated with decreased bacterial populations as indicated by phospholipid fatty acid (PLFA) concentrations. However, relatively minor impacts on overall community composition were indicated. Porewater methane concentrations and isotopic data indicated a difference in the occurrence of methane oxidation between the two sites. This study confirms temporally limited transport of contaminants from highly impacted regions as a vector for contaminants within the harbour and the impact on microbial carbon cycling and bed stability.

Using a shear-cell/flow-cell combination integrated with an inverted microscope, the behaviour of Hamilton Harbour sediments was studied mixed with three different amendments: alum, chitosan (both coagulants) and a polyacrylamide (a flocculant). Samples from the shear cell were drawn into the flow cell, where floc structure and size were assessed throughout the floc formation and breakage stages using computer image analysis. Settling velocity, density and porosity were also assessed, with results suggesting that amendment addition may be an effective method for the management of high-turbidity environments, provided there are no toxicological effects. In an assessment of performance, it was found that the polyacrylamide flocculant showed the greatest promise in reducing turbidity levels as it produced the largest flocs with the highest settling velocity. Although more prone to break-up, these flocs still remained larger than those formed with alum or chitosan at the same shear. All flocs, regardless of amendment, broke up due to a fracture mechanism rather than by microscale erosion. By improving our understanding of how these amendments may influence floc properties and behaviours, more effective management tools may be developed for the remediation and control of high-turbidity aquatic environments.

The effect of solids retention time (SRT) (4-20 d) on sludge floc structure, size distribution and morphology in laboratory-scale sequencing batch reactors receiving a glucose-based synthetic wastewater was studied using image analysis in a long-term experiment over one year. Floc size distribution (>10 microm) could be characterized by a log-normal model for no bulking situations, but a bi-modal distribution of floc size was observed for modest bulking situations. In each operating cycle of the SBRs, the variation in food /microorganisms ratio (0.03-1.0) had no significant influence on floc size distribution and morphology. The results from a long-term study over one year showed that no clear relationship existed between SRT and median floc size based on frequency. However, sludge flocs at the lower SRTs (4-9 d) were much more irregular and more variable in size with time than those at higher SRTs (16 and 20 d). The level of effluent-suspended solids at lower SRTs was higher than that at higher SRTs.

Activated sludge floc from a wastewater treatment system was characterized, with regard to principal structural, chemical, and microbiological components and properties, in relation to contaminant-colloid associations and settling. Multiscale analytical microscopies, in conjunction with multimethod sample preparations, were used correlatively to characterize diverse colloidal matrices within microbial floc. Transmission electron microscopy, in conjunction with energy dispersive spectroscopy (EDS), revealed specific associations of contaminant heavy metals with individual bacterial cells and with extracellular polymeric substances (EPS). Floc structure was mapped from the level of gross morphology down to the nano-scale, and flocs were described with respect to settling properties, size, shape, density, porosity, bound water content, and EPS chemical composition; gross surface properties were also measured for correlation with principal floc features. Compartmentalization results based on 171 EDS analyses and representative high-resolution images showed that nano-scale agglomerations of (i) silver (100%) and (ii) zinc (91%) were confined almost entirely to EPS matrices while (iii) Pb (100%) was confined to intracellular granules and (iv) aluminum was partitioned between EPS matrices (41%) and intracellular matrices (59%). The results suggest that engineered changes in microbial physiology and/or in macromolecular EPS composition may influence metal removal efficiencies.

Interparticle interactions affecting the stability of sludge flocs taken from laboratory-scale sequencing batch reactors at different solids retention times (SRTs) were investigated in batch experiments by varying the pH, ionic strength, cation valence, and urea and ethylenediaminetetraacetate concentrations of suspending solutions. The ultrastructure of sludge floc surfaces was observed by transmission electron microscopy. Changes in dissociation constants of sludge flocs under different conditions indicated that ionic interactions and hydrogen bonds held flocs together and compensated for the negative influence of electrostatic interactions on the stability of sludge flocs. Ionic interactions and hydrogen bonds were two dominant forces that maintained the stability of sludge flocs at lower SRTs; other mechanisms, such as physical enmeshment and van der Waals and/or hydrophobic interactions, were more important in controling the stability of sludge flocs at higher SRTs. Sludge flocs at higher SRTs (16 and 20 days) were physically more stable than those at lower SRTs (4 and 9 days). A conceptual model of floc structure, based on interparticle interactions, for describing the stability of sludge flocs is proposed. The floc matrix is proposed to consist of two physically distinct regions that are defined by the arrangement of extracellular polymeric substances (EPS). These are likely to be differentially affected by the agents applied to manipulate interparticle forces. Thus, the heterogeneity in the packing of and the type of EPS reflects the stability of the floc.

The urban continuum, as it applies to sediments and associated contaminants, represents the area over/through which sediments are conveyed from a depositional or eroded surface to a treatment system and/or receiving water body. This study has focused on the changing physical characteristics of the sediment, with an emphasis on flocculation/aggregation, as it progresses through the urban continuum. The sediments of the urban continuum are found to change from an unflocculated state on the street, to a flocculated state in the surface runoff to a very large floc form in the sewer system. The high organic content in the sewers contributes to the large floc size. The structure of the flocs and the flow regime of the receiving water will dictate the fate of the sediment following a combined sewer overflow. Probability distributions fitted to the distributions of each sediment type (compartment) confirmed significant differences in the sediment population sizes. Bulk and individual particle settling velocity experiments also revealed substantial differences between compartments. Sewer flocs were found to be of low density, with high porosity, water and organic content and with settling velocities which increase with floc size.

Correlative microscopy was applied to study the influence of solids retention time on activated sludge floc structure. Conventional optical microscopy revealed flocs at lower SRTs (4 and 9 days) to be irregular in shape while flocs at higher SRTs (16 and 20 days) had a more spherical and compact structure. Flocs were examined by environmental scanning electron microscopy (ESEM) and by transmission electron microscopy (TEM) coupled with energy-dispersive spectroscopy (EDS). Distinctive differences in floc structure and the arrangement of EPS were revealed. Flocs from higher SRTs were less hydrated and were found to possess a dense EPS layer that covers much of the surface. Extracellular osmiophilic granules present in these flocs indicate that the cells at the higher SRT may produce more lipid-like material. This EPS layer appears to decrease the floc surface roughness and protects the interior cells from disruption by changes in the external environment. Sludge flocs at higher SRTs were found to be physically more stable than those at lower SRTs.

Experiments on the erosion of a bed of kaolinite were carried out in a rotating circular flume. Each experiment was carried out using the stratified bed which resulted from the previous experiment. Changes in suspended sediment concentrations during the experiments were explained by the history of the deposition. The sequence of experiments showed how the rate of erosion and the amount eroded reflected the structure of the bed and that of the individual flocs which created it. Results suggest that modelling of sediment/contaminant transport needs to account for the manner in which deposition took place.

Experiments were conducted in an annular flume using a commercially available kaolinite clay as well as contaminated bed sediment from Hamilton Harbour (Ontario) to assess their stability against erosion. Critical shear stress for erosion was measured under different conditions of bed formation (quiescently deposited beds and shear deposited beds) as well as with and without the presence of a biostabilized bed. Results suggest that a biostabilized bed and a bed formed under a flowing condition, similar to a river scenario, will be more resistant against erosion than will a non-biostabilized bed and a bed formed under quiescent conditions. Up to three cycles of erosion and flocculation/deposition were observed to occur within one experiment. These results suggest that the depositional history and biostabilization of river bed sediments need to be seriously considered within sediment and contaminant transport models if meaningful estimates of sediment and contaminant source, fate and effect are to be generated and used for the management of our aquatic ecosystems.

The influence of sludge retention time (SRT) on the extracellular polymeric substances (EPS) and physicochemical properties (hydrophobicity and surface charge) of sludge was studied using laboratory-scale sequencing batch reactors (SBRs) fed a synthetic wastewater containing glucose and inorganic salts. Sludge surfaces were more hydrophobic (larger contact angle) and less negatively charged at higher SRTs (16 and 20 d) than at lower SRTs (4 and 9 d). The ratio of proteins to carbohydrates within the EPS of the sludges increased as the SRT increased from 4 to 12 d corresponding to the changes in the physicochemical properties of the sludge. The protein:carbohydrate ratio remained constant at SRTs of 16 and 20 d. A transition in sludge properties appeared to occur between the upper range of low- (9 d) and lower range of high-SRTs. The total EPS content, however, was independent of the SRT. A higher sludge volume index (SVI), an indication of poorer settleability or compression, was associated with a larger amount of total EPS but no significant correlation between SVI and the surface properties of sludge was observed. A more hydrophobic and less negatively charged surface corresponded to lower levels of ESS. These results indicate that it is the surface properties, hydrophobicity, surface charge and composition of EPS, of sludge, rather than the quantity of EPS, that govern bioflocculation. In contrast, the EPS content is more important in controlling the settleability of sludge.

A nondestructive stabilization technique for the characterization of microbial flocs which permits the application of correlative microscopic techniques is described. Flocs embedded in agarose are retained in a porous, resilient medium which allows for the transport, staining, washing, and subsampling of the flocculated material directly within a plankton chamber with minimal or no destructive forces. A single agarose disc can be subdivided into numerous sections for analysis by several microscope types and associated techniques.

Metal (Pb, Cu and Zn) partitioning in six separated sediment size fractions (<8, 8-12, 12-19, 19-31, 31-42, 42-60 microm) of river bed sediment have been analyzed by sequential extraction. The concentrations of some major elements (Si, Al, Ca, Mg, K, Na, Fe, Mn and P), and organic and inorganic C were determined to correlate the elemental composition of the sediment with metal speciation and grain size. Results show that Zn and Pb concentrations increase with decreasing grain size. For Big Creek and Big Otter Creek, respectively, the highest concentrations of Zn (326 and 230 mg kg(-1)) and Pb (158 and 67 mg kg(-1)) were found in the smallest (<8 microm) fraction, whereas the Cu levels (619 and 1281 mg kg(-1)) were most abundant in the second smallest (8-12 microm) fraction. The major accumulative phases for Cu, Zn and Pb were carbonates, Fe/Mn oxides and organic matter, but the relative importance of each phase varied for individual metals and grain sizes. The extraction data show increasing potential bioavailability of metals with decreasing grain size. Estimates of metal yields in the study catchments suggest that over 80% of the metal yield in sediment smaller than 63 microm is associated with solids smaller than 31 microm.