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1. A broadly cross-reactive monoclonal antibody against hepatitis E virus capsid antigen

Author

Kubickova, Barbara, Schenk, Jörg A., Ramm, Franziska, Markuškienė, Kornelija, Reetz, Jochen, Dremsek, Paul, Tamosiunas, Paulius Lukas, Cepulyte, Laima, Trinh, Hoai Anh, Scholz, Johannes, Memczak, Henry, Hovestädt, Marc, Ryll, René, Petraityte-Burneikiene, Rasa, Corman, Victor M., Andersson, Anika, Becher, Dietmar, Groschup, Martin H., Kubick, Stefan, Sellrie, Frank, Johne, Reimar, and Ulrich, Rainer G.

Published
2021
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10. Detection of rat hepatitis E virus in wild Norway rats (Rattus norvegicus) and Black rats (Rattus rattus) from 11 European countries

Author

Ryll, René, primary, Bernstein, Samuel, additional, Heuser, Elisa, additional, Schlegel, Mathias, additional, Dremsek, Paul, additional, Zumpe, Maxi, additional, Wolf, Sandro, additional, Pépin, Michel, additional, Bajomi, Daniel, additional, Müller, Gabi, additional, Heiberg, Ann-Charlotte, additional, Spahr, Carina, additional, Lang, Johannes, additional, Groschup, Martin H., additional, Ansorge, Hermann, additional, Freise, Jona, additional, Guenther, Sebastian, additional, Baert, Kristof, additional, Ruiz-Fons, Francisco, additional, Pikula, Jiri, additional, Knap, Nataša, additional, Tsakmakidis, Ιoannis, additional, Dovas, Chrysostomos, additional, Zanet, Stefania, additional, Imholt, Christian, additional, Heckel, Gerald, additional, Johne, Reimar, additional, and Ulrich, Rainer G., additional

Published
2017
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11. Detection of rat hepatitis E virus in wild Norway rats (Rattus norvegicus) and Black rats (Rattus rattus) from 11 European countries

Author

German Center for Infection Research, Ryll, René, Bernstein, Samuel, Heuser, Elisa, Schlegel, Mathias, Dremsek, Paul, Zumpe, Maxi, Wolf, Sandro, Pépin, Michel, Bajomi, Daniel, Müller, Gabi, Heiberg, Ann-Charlotte, Spahr, Carina, Lang, Johannes, Groschup, Martin H., Ansorge, Hermann, Freise, Jona, Guenther, Sebastian, Baert, Kristof, Ruiz-Fons, Francisco, Pikula, Jiri, Knap, Nataša, Tsakmakidis, Ioannis., Dovas, Chrysostomos, Zanet, Stefania, Imholt, Christian, Heckel, Gerald, Johne, Reimar, Ulrich, Rainer G., German Center for Infection Research, Ryll, René, Bernstein, Samuel, Heuser, Elisa, Schlegel, Mathias, Dremsek, Paul, Zumpe, Maxi, Wolf, Sandro, Pépin, Michel, Bajomi, Daniel, Müller, Gabi, Heiberg, Ann-Charlotte, Spahr, Carina, Lang, Johannes, Groschup, Martin H., Ansorge, Hermann, Freise, Jona, Guenther, Sebastian, Baert, Kristof, Ruiz-Fons, Francisco, Pikula, Jiri, Knap, Nataša, Tsakmakidis, Ioannis., Dovas, Chrysostomos, Zanet, Stefania, Imholt, Christian, Heckel, Gerald, Johne, Reimar, and Ulrich, Rainer G.

Abstract

Rat hepatitis E virus (HEV) is genetically only distantly related to hepeviruses found in other mammalian reservoirs and in humans. It was initially detected in Norway rats (Rattus norvegicus) from Germany, and subsequently in rats from Vietnam, the USA, Indonesia, China, Denmark and France. Here, we report on a molecular survey of Norway rats and Black rats (Rattus rattus) from 12 European countries for ratHEV and human pathogenic hepeviruses. RatHEV-specific real-time and conventional RT-PCR investigations revealed the presence of ratHEV in 63 of 508 (12.4%) rats at the majority of sites in 11 of 12 countries. In contrast, a real-time RT-PCR specific for human pathogenic HEV genotypes 1–4 and a nested broad-spectrum (NBS) RT-PCR with subsequent sequence determination did not detect any infections with these genotypes. Only in a single Norway rat from Belgium a rabbit HEV-like genotype 3 sequence was detected. Phylogenetic analysis indicated a clustering of all other novel Norway and Black rat-derived sequences with ratHEV sequences from Europe, the USA and a Black rat-derived sequence from Indonesia within the proposed ratHEV genotype 1. No difference in infection status was detected related to age, sex, rat species or density of human settlements and zoological gardens. In conclusion, our investigation shows a broad geographical distribution of ratHEV in Norway and Black rats from Europe and its presence in all settlement types investigated.

Published
2017

14. Age-related and regional differences in the prevalence of hepatitis E virus-specific antibodies in pigs in Germany

Author

Krumbholz, Andi, primary, Joel, Sebastian, additional, Neubert, Anne, additional, Dremsek, Paul, additional, Dürrwald, Ralf, additional, Johne, Reimar, additional, Hlinak, Andreas, additional, Walther, Mario, additional, Lange, Jeannette, additional, Wutzler, Peter, additional, Sauerbrei, Andreas, additional, Ulrich, Rainer G., additional, and Zell, Roland, additional

Published
2013
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15. The Interferon Stimulated Gene 12 Inactivates Vasculoprotective Functions of NR4A Nuclear Receptors

Author

Papac-Milicevic, Nikolina, primary, Breuss, Johannes M., additional, Zaujec, Jan, additional, Ryban, Lubos, additional, Plyushch, Tatiana, additional, Wagner, Gabriel A., additional, Fenzl, Sabine, additional, Dremsek, Paul, additional, Cabaravdic, Muris, additional, Steiner, Marianne, additional, Glass, Christopher K., additional, Binder, Christoph J., additional, Uhrin, Pavel, additional, and Binder, Bernd R., additional

Published
2012
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16. Seroprevalence study in forestry workers from eastern Germany using novel genotype 3- and rat hepatitis E virus-specific immunoglobulin G ELISAs

Author

Dremsek, Paul, primary, Wenzel, Jürgen J., additional, Johne, Reimar, additional, Ziller, Mario, additional, Hofmann, Jörg, additional, Groschup, Martin H., additional, Werdermann, Sandra, additional, Mohn, Ulrich, additional, Dorn, Silvia, additional, Motz, Manfred, additional, Mertens, Marc, additional, Jilg, Wolfgang, additional, and Ulrich, Rainer G., additional

Published
2011
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17. Novel Hepatitis E Virus Genotype in Norway Rats, Germany

Author

Johne, Reimar, primary, Heckel, Gerald, additional, Dremsek, Paul, additional, Plenge-Bönig, Anita, additional, Kindler, Eveline, additional, Maresch, Christina, additional, Reetz, Jochen, additional, Schielke, Anika, additional, and Ulrich, Rainer G., additional

Published
2011
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18. Detection of rat hepatitis E virus in wild Norway rats (Rattus norvegicus) and Black rats (Rattus rattus) from 11 European countries

Author

Ryll, René, Bernstein, Samuel, Heuser, Elisa, Schlegel, Mathias, Dremsek, Paul, Zumpe, Maxi, Wolf, Sandro, Pépin, Michel, Bajomi, Daniel, Müller, Gabi, Heiberg, Ann-Charlotte, Spahr, Carina, Lang, Johannes, Groschup, Martin H., Ansorge, Hermann, Freise, Jona, Guenther, Sebastian, Baert, Kristof, Ruiz-Fons, Francisco, Pikula, Jiri, Knap, Natasa, Tsakmakidis, Ioannis, Dovas, Chrysostomos, Zanet, Stefania, Imholt, Christian, Heckel, Gerald, Johne, Reimar, and Ulrich, Rainer G.

Subjects
570 Life sciences, biology, 3. Good health
Abstract

Rat hepatitis E virus (HEV) is genetically only distantly related to hepeviruses found in other mammalian reservoirs and in humans. It was initially detected in Norway rats (Rattus norvegicus) from Germany, and subsequently in rats from Vietnam, the USA, Indonesia, China, Denmark and France. Here, we report on a molecular survey of Norway rats and Black rats (Rattus rattus) from 12 European countries for ratHEV and human pathogenic hepeviruses. RatHEV-specific real-time and conventional RT-PCR investigations revealed the presence of ratHEV in 63 of 508 (12.4%) rats at the majority of sites in 11 of 12 countries. In contrast, a real-time RT-PCR specific for human pathogenic HEV genotypes 1–4 and a nested broadspectrum (NBS) RT-PCR with subsequent sequence determination did not detect any infections with these genotypes. Only in a single Norway rat from Belgium a rabbit HEV-like genotype 3 sequence was detected. Phylogenetic analysis indicated a clustering of all other novel Norway and Black rat-derived sequences with ratHEV sequences from Europe, the USA and a Black rat-derived sequence from Indonesia within the proposed ratHEV genotype 1. No difference in infection status was detected related to age, sex, rat species or density of human settlements and zoological gardens. In conclusion, our investigation shows a broad geographical distribution of ratHEV in Norway and Black rats from Europe and its presence in all settlement types investigated.

19. Different Outcomes of Experimental Hepatitis E Virus Infection in Diverse Mouse Strains, Wistar Rats, and Rabbits.

Author

Schlosser, Josephine, Dähnert, Lisa, Dremsek, Paul, Tauscher, Kerstin, Fast, Christine, Ziegler, Ute, Gröner, Albrecht, Ulrich, Rainer G, Groschup, Martin H, and Eiden, Martin

Subjects
HEPATITIS E virus, LABORATORY rats, LABORATORY rabbits, IMMUNE response, GENOTYPES
Abstract

Hepatitis E virus (HEV) is the causative agent of acute hepatitis E in humans in developing countries, but autochthonous cases of zoonotic genotype 3 (HEV-3) infection also occur in industrialized countries. In contrast to swine, rats, and rabbits, natural HEV infections in mice have not yet been demonstrated. The pig represents a well-established large animal model for HEV-3 infection, but a suitable small animal model mimicking natural HEV-3 infection is currently missing. Therefore, we experimentally inoculated C57BL/6 mice (wild-type, IFNAR−/−, CD4−/−, CD8−/−) and BALB/c nude (nu/nu) mice, Wistar rats, and European rabbits with a wild boar-derived HEV-3 strain and monitored virus replication and shedding, as well as humoral immune responses. HEV RNA and anti-HEV antibodies were detected in one and two out of eight of the rats and all rabbits inoculated, respectively, but not in any of the mouse strains tested. Remarkably, immunosuppressive dexamethasone treatment of rats did not enhance their susceptibility to HEV infection. In rabbits, immunization with recombinant HEV-3 and ratHEV capsid proteins induced protection against HEV-3 challenge. In conclusion, the rabbit model for HEV-3 infection may serve as a suitable alternative to the non-human primate and swine models, and as an appropriate basis for vaccine evaluation studies. [ABSTRACT FROM AUTHOR]

Published
2019
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20. Retrospective study on the utility of optical genome mapping as a follow-up method in genetic diagnostics.

Author

Dremsek P, Schachner A, Reischer T, Krampl-Bettelheim E, Bettelheim D, Vrabel S, Delissen Z, Pfeifer M, Weil B, Bajtela R, Hengstschläger M, Laccone F, and Neesen J

Subjects
Humans, Retrospective Studies, Female, Male, Exome Sequencing methods, Karyotyping methods, Follow-Up Studies, Genomic Structural Variation genetics, Genome, Human genetics, Genetic Testing methods, Chromosome Mapping methods
Abstract

Background: Current standard-of-care (SOC) methods for genetic testing are capable of resolving deletions and sequence variants, but they mostly fail to provide information on the breakpoints of duplications and balanced structural variants (SV). However, this information may be necessary for their clinical assessment, especially if the carrier's phenotype is difficult to assess and/or carrier analysis of relatives is not viable. A promising approach to solving such challenging cases arises with access to optical genome mapping (OGM) but has not been systematically explored as of yet., Methods: In this retrospective study, we evaluated diagnostic cases from a 1-year period (2023) in which an SV discovery by SOC methods (microarray, karyotyping and whole-exome sequencing) was followed up by OGM, with the objective to unlock clinically relevant information about the SV., Results: Seven cases were shown by SOC methods to bear potential pathogenic SVs and were consequently followed up by OGM. Of these, six were solved by the additional use of OGM alone. One case required sequencing after OGM analysis to further specify the SV's breakpoints. In all seven cases, OGM was crucial for determining the clinical relevance of the detected SV., Conclusion: This study describes the use of OGM as a valuable method for characterising duplications and balanced SVs. Often, this additional information does not add to the quality of a clinical report. However, for a subset of patients, these data are critical, especially in the prenatal setting or when no familial analyses are possible., Competing Interests: Competing interests: None declared., (© Author(s) (or their employer(s)) 2025. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ Group.)

Published
2025
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