2,028 results on '"Drakeley Chris"'
Search Results
2. Evidence for a role of Anopheles stephensi in the spread of drug- and diagnosis-resistant malaria in Africa.
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Emiru, Tadele, Getachew, Dejene, Murphy, Maxwell, Sedda, Luigi, Ejigu, Legesse, Bulto, Mikiyas, Byrne, Isabel, Demisse, Mulugeta, Abdo, Melat, Chali, Wakweya, Elliott, Aaron, Vickers, Eric, Aranda-Díaz, Andrés, Alemayehu, Lina, Behaksera, Sinknesh, Jebessa, Gutema, Dinka, Hunduma, Tsegaye, Tizita, Teka, Hiwot, Chibsa, Sheleme, Mumba, Peter, Girma, Samuel, Hwang, Jimee, Yoshimizu, Melissa, Sutcliffe, Alice, Taffese, Hiwot, Bayissa, Gudissa, Zohdy, Sarah, Tongren, Jon, Drakeley, Chris, Greenhouse, Bryan, Bousema, Teun, and Tadesse, Fitsum
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Animals ,Humans ,Malaria ,Anopheles ,Mosquito Vectors ,Malaria ,Falciparum ,Ethiopia - Abstract
Anopheles stephensi, an Asian malaria vector, continues to expand across Africa. The vector is now firmly established in urban settings in the Horn of Africa. Its presence in areas where malaria resurged suggested a possible role in causing malaria outbreaks. Here, using a prospective case-control design, we investigated the role of An. stephensi in transmission following a malaria outbreak in Dire Dawa, Ethiopia in April-July 2022. Screening contacts of patients with malaria and febrile controls revealed spatial clustering of Plasmodium falciparum infections around patients with malaria in strong association with the presence of An. stephensi in the household vicinity. Plasmodium sporozoites were detected in these mosquitoes. This outbreak involved clonal propagation of parasites with molecular signatures of artemisinin and diagnostic resistance. To our knowledge, this study provides the strongest evidence so far for a role of An. stephensi in driving an urban malaria outbreak in Africa, highlighting the major public health threat posed by this fast-spreading mosquito.
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- 2023
3. Household-level effects of seasonal malaria chemoprevention in the Gambia
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Soremekun, Seyi, Conteh, Bakary, Nyassi, Abdoullah, Soumare, Harouna M., Etoketim, Blessed, Ndiath, Mamadou Ousmane, Bradley, John, D’Alessandro, Umberto, Bousema, Teun, Erhart, Annette, Moreno, Marta, and Drakeley, Chris
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- 2024
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4. Flexible and cost-effective genomic surveillance of P. falciparum malaria with targeted nanopore sequencing
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de Cesare, Mariateresa, Mwenda, Mulenga, Jeffreys, Anna E., Chirwa, Jacob, Drakeley, Chris, Schneider, Kammerle, Mambwe, Brenda, Glanz, Karolina, Ntalla, Christina, Carrasquilla, Manuela, Portugal, Silvia, Verity, Robert J., Bailey, Jeffrey A., Ghinai, Isaac, Busby, George B., Hamainza, Busiku, Hawela, Moonga, Bridges, Daniel J., and Hendry, Jason A.
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- 2024
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5. Malaria seroepidemiology in very low transmission settings in the Peruvian Amazon
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Fernandez-Camacho, Bryan, Peña-Calero, Brian, Guillermo-Roman, Martina, Ruiz-Cabrejos, Jorge, Barboza, Jose Luis, Bartolini-Arana, Lucia, Barja-Ingaruca, Antony, Rodriguez-Ferrucci, Hugo, Soto-Calle, Veronica E., Nelli, Luca, Byrne, Isabel, Hill, Monica, Dumont, Elin, Grignard, Lynn, Tetteh, Kevin, Wu, Lindsey, Llanos-Cuentas, Alejandro, Drakeley, Chris, Stresman, Gillian, and Carrasco-Escobar, Gabriel
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- 2024
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6. Antibodies to repeat-containing antigens in Plasmodium falciparum are exposure-dependent and short-lived in children in natural malaria infections.
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Raghavan, Madhura, Kalantar, Katrina L, Duarte, Elias, Teyssier, Noam, Takahashi, Saki, Kung, Andrew F, Rajan, Jayant V, Rek, John, Tetteh, Kevin KA, Drakeley, Chris, Ssewanyana, Isaac, Rodriguez-Barraquer, Isabel, Greenhouse, Bryan, and DeRisi, Joseph L
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Humans ,Plasmodium falciparum ,Malaria ,Malaria ,Falciparum ,Protozoan Proteins ,Antibodies ,Protozoan ,Antigens ,Protozoan ,Epitopes ,Adult ,Child ,P. falciparum ,antibodies ,antigen profiling ,human ,immunology ,infectious disease ,inflammation ,malaria ,microbiology ,natural immunity ,phage display ,repeat regions ,Vector-Borne Diseases ,Immunization ,Rare Diseases ,Biotechnology ,Pediatric ,HIV/AIDS ,Infectious Diseases ,2.1 Biological and endogenous factors ,Aetiology ,Infection ,Good Health and Well Being ,Human ,P ,falciparum ,Biochemistry and Cell Biology - Abstract
Protection against Plasmodium falciparum, which is primarily antibody-mediated, requires recurrent exposure to develop. The study of both naturally acquired limited immunity and vaccine induced protection against malaria remains critical for ongoing eradication efforts. Towards this goal, we deployed a customized P. falciparum PhIP-seq T7 phage display library containing 238,068 tiled 62-amino acid peptides, covering all known coding regions, including antigenic variants, to systematically profile antibody targets in 198 Ugandan children and adults from high and moderate transmission settings. Repeat elements - short amino acid sequences repeated within a protein - were significantly enriched in antibody targets. While breadth of responses to repeat-containing peptides was twofold higher in children living in the high versus moderate exposure setting, no such differences were observed for peptides without repeats, suggesting that antibody responses to repeat-containing regions may be more exposure dependent and/or less durable in children than responses to regions without repeats. Additionally, short motifs associated with seroreactivity were extensively shared among hundreds of antigens, potentially representing cross-reactive epitopes. PfEMP1 shared motifs with the greatest number of other antigens, partly driven by the diversity of PfEMP1 sequences. These data suggest that the large number of repeat elements and potential cross-reactive epitopes found within antigenic regions of P. falciparum could contribute to the inefficient nature of malaria immunity.
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- 2023
7. Plasmodium falciparum gametocyte carriage in longitudinally monitored incident infections is associated with duration of infection and human host factors
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Andolina, Chiara, Ramjith, Jordache, Rek, John, Lanke, Kjerstin, Okoth, Joseph, Grignard, Lynn, Arinaitwe, Emmanuel, Briggs, Jessica, Bailey, Jeffrey, Aydemir, Ozkan, Kamya, Moses R, Greenhouse, Bryan, Dorsey, Grant, Staedke, Sarah G, Drakeley, Chris, Jonker, Marianne, and Bousema, Teun
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Medical Microbiology ,Biomedical and Clinical Sciences ,Clinical Sciences ,Clinical Research ,Vector-Borne Diseases ,Prevention ,Infectious Diseases ,Malaria ,Rare Diseases ,Aetiology ,2.2 Factors relating to the physical environment ,Infection ,Good Health and Well Being ,Animals ,Humans ,Plasmodium falciparum ,Malaria ,Falciparum ,Asymptomatic Infections ,Uganda ,Culicidae - Abstract
Malaria transmission depends on the presence of Plasmodium gametocytes that are the only parasite life stage that can infect mosquitoes. Gametocyte production varies between infections and over the course of infections. Infection duration is highly important for gametocyte production but poorly quantified. Between 2017 and 2019 an all-age cohort of individuals from Tororo, eastern Uganda was followed by continuous passive and routine assessments. We longitudinally monitored 104 incident infections from 98 individuals who were sampled once every 28 days and on any day of symptoms. Among infections that lasted ≥ 3 months, gametocyte appearance was near-universal with 96% of infections having detectable gametocytes prior to clearance. However, most infections were of much shorter duration; 55.7% of asymptomatic infections were detected only once. When considering all asymptomatic infections, regardless of their duration, only 36.3% had detectable gametocytes on at least one time-point prior to parasite clearance. Infections in individuals with sickle-cell trait (HbAS) were more likely to have gametocytes detected (Hazard Rate (HR) = 2.68, 95% CI 1.12, 6.38; p = 0.0231) and had gametocytes detected at higher densities (Density Ratio (DR) = 9.19, 95% CI 2.79, 30.23; p = 0.0002) compared to infections in wildtype (HbAA) individuals. Our findings suggest that a large proportion of incident infections is too short in duration and of too low density to contribute to onward transmission.
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- 2023
8. East Africa International Center of Excellence for Malaria Research: Summary of Key Research Findings
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Nankabirwa, Joaniter I, Rek, John, Arinaitwe, Emmanuel, Namuganga, Jane Frances, Nsobya, Sam L, Asua, Victor, Mawejje, Henry D, Epstein, Adrienne, Greenhouse, Bryan, Rodriguez-Barraquer, Isabel, Briggs, Jessica, Krezanoski, Paul J, Rosenthal, Philip J, Conrad, Melissa, Smith, David, Staedke, Sarah G, Drakeley, Chris, Bousema, Teun, Andolina, Chiara, Donnelly, Martin J, Kamya, Moses R, and Dorsey, Grant
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Medical Microbiology ,Biomedical and Clinical Sciences ,Clinical Sciences ,Vector-Borne Diseases ,HIV/AIDS ,Prevention ,Rare Diseases ,Malaria ,Clinical Research ,Infectious Diseases ,Prevention of disease and conditions ,and promotion of well-being ,3.2 Interventions to alter physical and biological environmental risks ,Infection ,Good Health and Well Being ,Adolescent ,Animals ,Antimalarials ,Artemisinins ,Carbamates ,Child ,Child ,Preschool ,Humans ,Insecticide Resistance ,Insecticide-Treated Bednets ,Insecticides ,Mosquito Control ,Mosquito Vectors ,Organophosphates ,Piperonyl Butoxide ,Pyrethrins ,Uganda ,Health Sciences ,Human Genome ,Biotechnology ,Pediatric ,Genetics ,2.6 Resources and infrastructure (aetiology) ,Aetiology ,Generic health relevance ,2.4 Surveillance and distribution ,Medical and Health Sciences ,Tropical Medicine ,Biomedical and clinical sciences ,Health sciences - Abstract
The Program for Resistance, Immunology, Surveillance, and Modeling of Malaria (PRISM) has been conducting malaria research in Uganda since 2010 to improve the understanding of the disease and measure the impact of population-level control interventions in the country. Here, we will summarize key research findings from a series of studies addressing routine health facility-based surveillance, comprehensive cohort studies, studies of the molecular epidemiology, and transmission of malaria, evaluation of antimalarial drug efficacy, and resistance across the country, and assessments of insecticide resistance. Among our key findings are the following. First, we found that in historically high transmission areas of Uganda, a combination of universal distribution of long-lasting insecticidal-treated nets (LLINs) and sustained indoor residual spraying (IRS) of insecticides lowered the malaria burden greatly, but marked resurgences occurred if IRS was discontinued. Second, submicroscopic infections are common and key drivers of malaria transmission, especially in school-age children (5-15 years). Third, markers of drug resistance have changed over time, with new concerning emergence of markers predicting resistance to artemisinin antimalarials. Fourth, insecticide resistance monitoring has demonstrated high levels of resistance to pyrethroids, appreciable impact of the synergist piperonyl butoxide to pyrethroid susceptibility, emerging resistance to carbamates, and complete susceptibility of malaria vectors to organophosphates, which could have important implications for vector control interventions. Overall, PRISM has yielded a wealth of information informing researchers and policy-makers on the malaria burden and opportunities for improved malaria control and eventual elimination in Uganda. Continued studies concerning all the types of surveillance discussed above are ongoing.
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- 2022
9. Effect of weekly fever-screening and treatment and monthly RDT testing and treatment on the infectious reservoir of malaria parasites in Burkina Faso: a cluster-randomised trial
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Collins, Katharine A, Ouedraogo, Alphonse, Guelbeogo, Wamdaogo Moussa, Soulama, Issiaka, Ouattara, Maurice S, Sombie, Salif, Ouedraogo, Nicolas, Coulibaly, Aboubacar S, Nombre, Apollinaire, Lanke, Kjerstin, Ramjith, Jordache, Awandu, Shehu S, Serme, Samuel S, Henry, Noelie, Stone, Will, Ouedraogo, Issa N, Diarra, Amidou, Holden, Tobias M, Sirima, Sodiomon B, Bradley, John, Soremekun, Seyi, Selvaraj, Prashanth, Gerardin, Jaline, Drakeley, Chris, Bousema, Teun, and Tiono, Alfred B
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- 2024
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10. Plasmodium falciparum gametocyte production correlates with genetic markers of parasite replication but is not influenced by experimental exposure to mosquito biting
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Blanken, Sara Lynn, Barry, Aissata, Lanke, Kjerstin, Guelbeogo, Moussa, Ouedraogo, Alphonse, Soulama, Issiaka, Coulibaly, Sam Aboubacar, Teelen, Karina, Graumans, Wouter, Dumont, Elin, Stone, Will, Ramjith, Jordache, Marti, Matthias, Andrade, Carolina M., Drakeley, Chris, Collins, Katharine, Tiono, Alfred, and Bousema, Teun
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- 2024
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11. Artemether–lumefantrine with or without single-dose primaquine and sulfadoxine–pyrimethamine plus amodiaquine with or without single-dose tafenoquine to reduce Plasmodium falciparum transmission: a phase 2, single-blind, randomised clinical trial in Ouelessebougou, Mali
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Mahamar, Almahamoudou, Smit, Merel J, Sanogo, Koualy, Sinaba, Youssouf, Niambele, Sidi M, Sacko, Adama, Dicko, Oumar M, Diallo, Makonon, Maguiraga, Seydina O, Sankaré, Yaya, Keita, Sekouba, Samake, Siaka, Dembele, Adama, Lanke, Kjerstin, ter Heine, Rob, Bradley, John, Dicko, Yahia, Traore, Sekou F, Drakeley, Chris, Dicko, Alassane, Bousema, Teun, and Stone, Will
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- 2024
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12. Extended malaria parasite clearance time in African children following artemisinin-combination therapy enhances transmission to Anopheles mosquitoes
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Beshir Khalid B, Sawa Patrick, Drakeley Chris J, Baidjoe Amrish Y, Mweresa Collins K, Yussuf Rahma U, Omar Sabah A, Hermsen Cornelus C, Shekalaghe Seif A, Schallig Henk DFH, Sauerwein Robert W, Sutherland Colin J, Hallett Rachel L, and Bousema Teun
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Arctic medicine. Tropical medicine ,RC955-962 ,Infectious and parasitic diseases ,RC109-216 - Published
- 2012
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13. Mass screening tools for glucose-6-phosphate dehydrogenase deficiency: validation of the WST8/1 -methoxy-PMS enzymatic assay in a highly malaria-endemic area in Uganda
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De Niz Mariana, Eziefula Alice C, Maiteki-Sebuguzi Catherine, Gonahasa Sam, DiLiberto Deborah, Tumwebaze Patrick, Staedke Sarah G, and Drakeley Chris J
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Arctic medicine. Tropical medicine ,RC955-962 ,Infectious and parasitic diseases ,RC109-216 - Published
- 2012
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14. Dynamics of asymptomatic Plasmodium falciparum and Plasmodium vivax infections and their infectiousness to mosquitoes in a low transmission setting of Ethiopia: a longitudinal observational study
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Hailemeskel, Elifaged, Tebeje, Surafel K., Ramjith, Jordache, Ashine, Temesgen, Lanke, Kjerstin, Behaksra, Sinknesh W., Emiru, Tadele, Tsegaye, Tizita, Gashaw, Abrham, Kedir, Soria, Chali, Wakweya, Esayas, Endashaw, Tafesse, Temesgen, Abera, Haile, Bulto, Mikiyas Gebremichael, Shumie, Girma, Petros, Beyene, Mamo, Hassen, Drakeley, Chris, Gadisa, Endalamaw, Bousema, Teun, and Tadesse, Fitsum G.
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- 2024
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15. Sustained clinical benefit of malaria chemoprevention with sulfadoxine-pyrimethamine (SP) in pregnant women in a region with high SP resistance markers
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Matambisso, Glória, Brokhattingen, Nanna, Maculuve, Sónia, Cístero, Pau, Mbeve, Henriques, Escoda, Anna, Bambo, Gizela, Cuna, Boaventura, Melembe, Cardoso, Ndimande, Nelo, Tetteh, Kevin K.A., Drakeley, Chris, Gamain, Benoit, Chitnis, Chetan, Chauhan, Virander, Quintó, Llorenç, Macete, Eusébio, and Mayor, Alfredo
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- 2024
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16. Zoonotic malaria requires new policy approaches to malaria elimination
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Fornace, Kimberly M., Drakeley, Chris J., Lindblade, Kim A., Jelip, Jenarun, and Ahmed, Kamruddin
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- 2023
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17. Assessing the impact of the “malaria supporters project” intervention to malaria control in the Brazilian Amazon: an interrupted time-series analysis
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Garcia, Klauss Kleydmann Sabino, Soremekun, Seyi, Bottomley, Christian, Abrahão, Amanda Amaral, de Miranda, Cristiano Barreto, Drakeley, Chris, Ramalho, Walter Massa, and Siqueira, André M.
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- 2023
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18. Serological evaluation of risk factors for exposure to malaria in a pre-elimination setting in Malaysian Borneo
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Byrne, Isabel, William, Timothy, Chua, Tock H., Patterson, Catriona, Hall, Tom, Tan, Mark, Chitnis, Chetan, Adams, John, Singh, Susheel K., Grignard, Lynn, Tetteh, Kevin K. A., Fornace, Kimberly M., and Drakeley, Chris J.
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- 2023
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19. Detecting temporal and spatial malaria patterns from first antenatal care visits
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Pujol, Arnau, Brokhattingen, Nanna, Matambisso, Glória, Mbeve, Henriques, Cisteró, Pau, Escoda, Anna, Maculuve, Sónia, Cuna, Boaventura, Melembe, Cardoso, Ndimande, Nelo, Munguambe, Humberto, Montaña, Júlia, Nhamússua, Lídia, Simone, Wilson, Tetteh, Kevin K. A., Drakeley, Chris, Gamain, Benoit, Chitnis, Chetan E., Chauhan, Virander, Quintó, Llorenç, Chidimatembue, Arlindo, Martí-Soler, Helena, Galatas, Beatriz, Guinovart, Caterina, Saúte, Francisco, Aide, Pedro, Macete, Eusébio, and Mayor, Alfredo
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- 2023
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20. Genome-wide genetic variation and molecular surveillance of drug resistance in Plasmodium falciparum isolates from asymptomatic individuals in Ouélessébougou, Mali
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Vanheer, Leen N., Mahamar, Almahamoudou, Manko, Emilia, Niambele, Sidi M., Sanogo, Koualy, Youssouf, Ahamadou, Dembele, Adama, Diallo, Makonon, Maguiraga, Seydina O., Phelan, Jody, Osborne, Ashley, Spadar, Anton, Smit, Merel J., Bousema, Teun, Drakeley, Chris, Clark, Taane G., Stone, William, Dicko, Alassane, and Campino, Susana
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- 2023
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21. Serological evaluation of the effectiveness of reactive focal mass drug administration and reactive vector control to reduce malaria transmission in Zambezi Region, Namibia: Results from a secondary analysis of a cluster randomised trial
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Wu, Lindsey, Hsiang, Michelle S, Prach, Lisa M, Schrubbe, Leah, Ntuku, Henry, Dufour, Mi-Suk Kang, Whittemore, Brooke, Scott, Valerie, Yala, Joy, Roberts, Kathryn W, Patterson, Catriona, Biggs, Joseph, Hall, Tom, Tetteh, Kevin KA, Gueye, Cara Smith, Greenhouse, Bryan, Bennett, Adam, Smith, Jennifer L, Katokele, Stark, Uusiku, Petrina, Mumbengegwi, Davis, Gosling, Roly, Drakeley, Chris, and Kleinschmidt, Immo
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Clinical Research ,Clinical Trials and Supportive Activities ,Vector-Borne Diseases ,Rare Diseases ,Infectious Diseases ,Malaria ,Infection ,Good Health and Well Being ,Serology ,Cluster randomised trials - Abstract
BackgroundDue to challenges in measuring changes in malaria at low transmission, serology is increasingly being used to complement clinical and parasitological surveillance. Longitudinal studies have shown that serological markers, such as Etramp5.Ag1, can reflect spatio-temporal differences in malaria transmission. However, these markers have yet to be used as endpoints in intervention trials.MethodsBased on data from a 2017 cluster randomised trial conducted in Zambezi Region, Namibia, evaluating the effectiveness of reactive focal mass drug administration (rfMDA) and reactive vector control (RAVC), this study conducted a secondary analysis comparing antibody responses between intervention arms as trial endpoints. Antibody responses were measured on a multiplex immunoassay, using a panel of eight serological markers of Plasmodium falciparum infection - Etramp5.Ag1, GEXP18, HSP40.Ag1, Rh2.2030, EBA175, PfMSP119, PfAMA1, and PfGLURP.R2.FindingsReductions in sero-prevalence to antigens Etramp.Ag1, PfMSP119, Rh2.2030, and PfAMA1 were observed in study arms combining rfMDA and RAVC, but only effects for Etramp5.Ag1 were statistically significant. Etramp5.Ag1 sero-prevalence was significantly lower in all intervention arms. Compared to the reference arms, adjusted prevalence ratio (aPR) for Etramp5.Ag1 was 0.78 (95%CI 0.65 - 0.91, p = 0.0007) in the rfMDA arms and 0.79 (95%CI 0.67 - 0.92, p = 0.001) in the RAVC arms. For the combined rfMDA plus RAVC intervention, aPR was 0.59 (95%CI 0.46 - 0.76, p < 0.0001). Significant reductions were also observed based on continuous antibody responses. Sero-prevalence as an endpoint was found to achieve higher study power (99.9% power to detect a 50% reduction in prevalence) compared to quantitative polymerase chain reaction (qPCR) prevalence (72.9% power to detect a 50% reduction in prevalence).InterpretationWhile the observed relative reduction in qPCR prevalence in the study was greater than serology, the use of serological endpoints to evaluate trial outcomes measured effect size with improved precision and study power. Serology has clear application in cluster randomised trials, particularly in settings where measuring clinical incidence or infection is less reliable due to seasonal fluctuations, limitations in health care seeking, or incomplete testing and reporting.FundingThis study was supported by Novartis Foundation (A122666), the Bill & Melinda Gates Foundation (OPP1160129), and the Horchow Family Fund (5,300,375,400).
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- 2022
22. The acquisition of humoral immune responses targeting Plasmodium falciparum sexual stages in controlled human malaria infections
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de Jong, Roos M, Alkema, Manon, Oulton, Tate, Dumont, Elin, Teelen, Karina, Nakajima, Rie, de Assis, Rafael Ramiro, Press, Kathleen W Dantzler, Ngotho, Priscilla, Tetteh, Kevin KA, Felgner, Phil, Marti, Matthias, Collins, Katharine A, Drakeley, Chris, Bousema, Teun, and Stone, Will JR
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Medical Microbiology ,Biomedical and Clinical Sciences ,Clinical Sciences ,Vaccine Related ,Vector-Borne Diseases ,Infectious Diseases ,Immunization ,Clinical Research ,Rare Diseases ,HIV/AIDS ,Biotechnology ,Malaria ,Aetiology ,2.1 Biological and endogenous factors ,Infection ,Good Health and Well Being ,Antibodies ,Protozoan ,Humans ,Immunity ,Humoral ,Malaria ,Falciparum ,Plasmodium falciparum ,malaria ,sexual stage ,gametocyte antigens ,antibody responses ,controlled human malaria infection ,Immunology ,Biochemistry and cell biology ,Genetics - Abstract
Individuals infected with P. falciparum develop antibody responses to intra-erythrocytic gametocyte proteins and exported gametocyte proteins present on the surface of infected erythrocytes. However, there is currently limited knowledge on the immunogenicity of gametocyte antigens and the specificity of gametocyte-induced antibody responses. In this study, we assessed antibody responses in participants of two controlled human malaria infection (CHMI) studies by ELISA, multiplexed bead-based antibody assays and protein microarray. By comparing antibody responses in participants with and without gametocyte exposure, we aimed to disentangle the antibody response induced by asexual and sexual stage parasites. We showed that after a single malaria infection, a significant anti-sexual stage humoral response is induced in malaria-naïve individuals, even after exposure to relatively low gametocyte densities (up to ~1,600 gametocytes/mL). In contrast to antibody responses to well-characterised asexual blood stage antigens that were detectable by day 21 after infection, responses to sexual stage antigens (including transmission blocking vaccine candidates Pfs48/45 and Pfs230) were only apparent at 51 days after infection. We found antigens previously associated with early gametocyte or anti-gamete immunity were highly represented among responses linked with gametocyte exposure. Our data provide detailed insights on the induction and kinetics of antibody responses to gametocytes and identify novel antigens that elicit antibody responses exclusively in individuals with gametocyte exposure. Our findings provide target identification for serological assays for surveillance of the malaria infectious reservoir, and support vaccine development by describing the antibody response to leading vaccine antigens after primary infection.
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- 2022
23. House design and risk of malaria, acute respiratory infection and gastrointestinal illness in Uganda: A cohort study
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Musiime, Alex K, Krezanoski, Paul J, Smith, David L, Kilama, Maxwell, Conrad, Melissa D, Otto, Geoffrey, Kyagamba, Patrick, Asiimwe, Jackson, Rek, John, Nankabirwa, Joaniter I, Arinaitwe, Emmanuel, Akol, Anne M, Kamya, Moses R, Staedke, Sarah G, Drakeley, Chris, Bousema, Teun, Lindsay, Steve W, Dorsey, Grant, and Tusting, Lucy S
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Medical Microbiology ,Biomedical and Clinical Sciences ,Clinical Sciences ,Prevention ,Malaria ,Vector-Borne Diseases ,Infectious Diseases ,Rare Diseases ,Clinical Research ,Aetiology ,2.2 Factors relating to the physical environment ,Infection ,Good Health and Well Being - Abstract
House construction is rapidly modernizing across Africa but the potential benefits for human health are poorly understood. We hypothesised that improvements to housing would be associated with reductions in malaria, acute respiratory infection (ARI) and gastrointestinal illness in an area of low malaria endemicity in Uganda. Data were analysed from a cohort study of male and female child and adult residents (n = 531) of 80 randomly-selected households in Nagongera sub-county, followed for 24 months (October 4, 2017 to October 31, 2019). Houses were classified as modern (brick walls, metal roof and closed eaves) or traditional (all other homes). Light trap collections of mosquitoes were done every two weeks in all sleeping rooms. Every four weeks, we measured malaria infection (using microscopy and qPCR to detect malaria parasites), incidence of malaria, ARI and gastrointestinal illness. We collected 15,780 adult female Anopheles over 7,631 nights. We collected 13,277 blood samples of which 10.2% (1,347) were positive for malaria parasites. Over 958 person years we diagnosed 38 episodes of uncomplicated malaria (incidence 0.04 episodes per person-year at risk), 2,553 episodes of ARI (incidence 2.7 episodes per person-year) and 387 episodes of gastrointestinal illness (incidence 0.4 episodes per person-year). Modern houses were associated with a 53% lower human biting rate compared to traditional houses (adjusted incidence rate ratio [aIRR] 0.47, 95% confidence interval [CI] 0.32-0.67, p
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- 2022
24. Remote bednet use monitoring to describe patterns of use and exposure to female Anopheles mosquitoes in an Ugandan cohort
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Krezanoski, Paul J, Rek, John, Musiime, Alex, Otto, Geoffrey, Kyagamba, Patrick, Rwatooro, Jackson Asiimwe, Walters, Kelly, Romanel, Alina, Arinaitwe, Emmanuel, Nankabirwa, Joaniter I, Drakeley, Chris J, Kamya, Moses, and Dorsey, Grant
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Medical Microbiology ,Biomedical and Clinical Sciences ,Biological Sciences ,Prevention ,Malaria ,Infectious Diseases ,Rare Diseases ,Vector-Borne Diseases ,Aetiology ,2.2 Factors relating to the physical environment ,Infection ,Good Health and Well Being ,Anopheles mosquito ,electronic bednet use monitoring ,human behavior ,insecticide-treated bednets ,malaria control - Abstract
BackgroundLong lasting insecticide-treated bednets (LLINs) are the most widely used tool for preventing malaria. There has been a plateau in progress in the highest burden African countries since 2015, leading to questions about the effectiveness of LLINs. In this study, remote LLIN use monitors were deployed in a cohort in Eastern Uganda to explore how LLIN use interacts with mosquito exposure.MethodsThe SmartNet study included 20 households from May to October 2019. SmartNet devices recorded, every 15 min, whether an LLIN was unfurled or folded up. Unannounced visits were used to assess SmartNet accuracy. Risk factors associated with poor LLIN use were assessed using generalized linear equations. Female Anopheles exposure was estimated by combining hourly probabilities of exposure from human landing catches and measures of density from biweekly CDC light traps in participants rooms. Mosquito exposure averted by LLINs was quantified using SmartNet measurements and age-related differences were estimated using generalized linear equations, adjusting for relevant covariates and household clustering.Results96 individuals contributed 5,640 SmartNet observation nights. In 126 unannounced visits, SmartNet had an area under the curve of 0.869 in classifying whether the LLIN was up or down. The rate of non-use was 13.5% of nights (95% CI: 12.6-14.3%). Compared to children under 5, non-use was 1.8 times higher (95% CI: 1.6-2.1; p < 0.001) in children 5-15 years and 2.6 times higher (95% CI: 2.2-3.1; p < 0.001) in participants aged 15- 30 years. LLIN use averted 50.3% of female Anopheles mosquito exposure (95% CI: 40.0-60.0%), with decreasing point estimates of efficacy across age groups: from 61.7% (95% CI: 42.6-80.7%) in children under 5 years to 48.0% (95% CI: 29.1-66.8%) in adults over 30.ConclusionsObjective monitors are accurate and can feasibly be deployed to obtain data about LLIN use. LLINs provided protection from only 50% of female Anopheles mosquito exposure in this cohort and protection was dependent upon age. In assessing the role of LLINs in malaria prevention it is crucial to consider the dynamics between mosquito exposure and LLIN use behaviors.
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- 2022
25. Within‐household clustering of genetically related Plasmodium falciparum infections in a moderate transmission area of Uganda
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Briggs, Jessica, Kuchta, Alison, Murphy, Max, Tessema, Sofonias, Arinaitwe, Emmanuel, Rek, John, Chen, Anna, Nankabirwa, Joaniter I, Drakeley, Chris, Smith, David, Bousema, Teun, Kamya, Moses, Rodriguez-Barraquer, Isabel, Staedke, Sarah, Dorsey, Grant, Rosenthal, Philip J, and Greenhouse, Bryan
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Vector-Borne Diseases ,HIV/AIDS ,Rare Diseases ,Infectious Diseases ,Malaria ,Clinical Research ,2.2 Factors relating to the physical environment ,Aetiology ,Infection ,Good Health and Well Being ,Adult ,Aged ,Aged ,80 and over ,Child ,Cohort Studies ,Genotype ,Humans ,Incidence ,Malaria ,Falciparum ,Microsatellite Repeats ,Middle Aged ,Plasmodium falciparum ,Uganda ,Young Adult ,Genotyping ,Transmission ,Microsatellite ,Clustering ,Molecular epidemiology ,Microbiology ,Medical Microbiology ,Public Health and Health Services ,Tropical Medicine - Abstract
BackgroundEvaluation of genetic relatedness of malaria parasites is a useful tool for understanding transmission patterns, but patterns are not easily detectable in areas with moderate to high malaria transmission. To evaluate the feasibility of detecting genetic relatedness in a moderate malaria transmission setting, relatedness of Plasmodium falciparum infections was measured in cohort participants from randomly selected households in the Kihihi sub-county of Uganda (annual entomological inoculation rate of 27 infectious bites per person).MethodsAll infections detected via microscopy or Plasmodium-specific loop mediated isothermal amplification from passive and active case detection during August 2011-March 2012 were genotyped at 26 microsatellite loci, providing data for 349 samples from 230 participants living in 80 households. Pairwise genetic relatedness was calculated using identity by state (IBS).ResultsAs expected, genetic diversity was high (mean heterozygosity [He] = 0.73), and the majority (76.5 %) of samples were polyclonal. Despite the high genetic diversity, fine-scale population structure was detectable, with significant spatiotemporal clustering of highly related infections. Although the difference in malaria incidence between households at higher (mean 1127 metres) versus lower elevation (mean 1015 metres) was modest (1.4 malaria cases per person-year vs. 1.9 per person-year, respectively), there was a significant difference in multiplicity of infection (2.2 vs. 2.6, p = 0.008) and, more strikingly, a higher proportion of highly related infections within households (6.3 % vs. 0.9 %, p = 0.0005) at higher elevation compared to lower elevation.ConclusionsGenetic data from a relatively small number of diverse, multiallelic loci reflected fine scale patterns of malaria transmission. Given the increasing interest in applying genetic data to augment malaria surveillance, this study provides evidence that genetic data can be used to inform transmission patterns at local spatial scales even in moderate transmission areas.
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- 2021
26. The impact of hotspot-targeted interventions on malaria transmission: study protocol for a cluster-randomized controlled trial
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Bousema Teun, Stevenson Jennifer, Baidjoe Amrish, Stresman Gillian, Griffin Jamie T, Kleinschmidt Immo, Remarque Edmond J, Vulule John, Bayoh Nabie, Laserson Kayla, Desai Meghna, Sauerwein Robert, Drakeley Chris, and Cox Jonathan
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Anopheles ,elimination ,epidemiology ,eradication ,falciparum ,heterogeneity ,immunology ,malaria ,molecular ,transmission ,Medicine (General) ,R5-920 - Abstract
Abstract Background Malaria transmission is highly heterogeneous in most settings, resulting in the formation of recognizable malaria hotspots. Targeting these hotspots might represent a highly efficacious way of controlling or eliminating malaria if the hotspots fuel malaria transmission to the wider community. Methods/design Hotspots of malaria will be determined based on spatial patterns in age-adjusted prevalence and density of antibodies against malaria antigens apical membrane antigen-1 and merozoite surface protein-1. The community effect of interventions targeted at these hotspots will be determined. The intervention will comprise larviciding, focal screening and treatment of the human population, distribution of long-lasting insecticide-treated nets and indoor residual spraying. The impact of the intervention will be determined inside and up to 500 m outside the targeted hotspots by PCR-based parasite prevalence in cross-sectional surveys, malaria morbidity by passive case detection in selected facilities and entomological monitoring of larval and adult Anopheles populations. Discussion This study aims to provide direct evidence for a community effect of hotspot-targeted interventions. The trial is powered to detect large effects on malaria transmission in the context of ongoing malaria interventions. Follow-up studies will be needed to determine the effect of individual components of the interventions and the cost-effectiveness of a hotspot-targeted approach, where savings made by reducing the number of compounds that need to receive interventions should outweigh the costs of hotspot-detection. Trial registration NCT01575613. The protocol was registered online on 20 March 2012; the first community was randomized on 26 March 2012.
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- 2013
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27. Determining seropositivity-A review of approaches to define population seroprevalence when using multiplex bead assays to assess burden of tropical diseases.
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Chan, YuYen, Fornace, Kimberly, Wu, Lindsey, Arnold, Benjamin F, Priest, Jeffrey W, Martin, Diana L, Chang, Michelle A, Cook, Jackie, Stresman, Gillian, and Drakeley, Chris
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Biological Sciences ,Medical and Health Sciences ,Tropical Medicine - Abstract
BackgroundSerological surveys with multiplex bead assays can be used to assess seroprevalence to multiple pathogens simultaneously. However, multiple methods have been used to generate cut-off values for seropositivity and these may lead to inconsistent interpretation of results. A literature review was conducted to describe the methods used to determine cut-off values for data generated by multiplex bead assays.Methodology/principal findingsA search was conducted in PubMed that included articles published from January 2010 to January 2020, and 308 relevant articles were identified that included the terms "serology", "cut-offs", and "multiplex bead assays". After application of exclusion of articles not relevant to neglected tropical diseases (NTD), vaccine preventable diseases (VPD), or malaria, 55 articles were examined based on their relevance to NTD or VPD. The most frequently applied approaches to determine seropositivity included the use of presumed unexposed populations, mixture models, receiver operating curves (ROC), and international standards. Other methods included the use of quantiles, pre-exposed endemic cohorts, and visual inflection points.Conclusions/significanceFor disease control programmes, seropositivity is a practical and easily interpretable health metric but determining appropriate cut-offs for positivity can be challenging. Considerations for optimal cut-off approaches should include factors such as methods recommended by previous research, transmission dynamics, and the immunological backgrounds of the population. In the absence of international standards for estimating seropositivity in a population, the use of consistent methods that align with individual disease epidemiological data will improve comparability between settings and enable the assessment of changes over time.
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- 2021
28. Simian malaria: a narrative review on emergence, epidemiology and threat to global malaria elimination
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Fornace, Kimberly M, Zorello Laporta, Gabriel, Vythilingham, Indra, Chua, Tock Hing, Ahmed, Kamruddin, Jeyaprakasam, Nantha K, de Castro Duarte, Ana Maria Ribeiro, Amir, Amirah, Phang, Wei Kit, Drakeley, Chris, Sallum, Maria Anice M, and Lau, Yee Ling
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- 2023
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29. Simulation of malaria epidemiology and control in the highlands of western Kenya
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Stuckey Erin M, Stevenson Jennifer C, Cooke Mary K, Owaga Chrispin, Marube Elizabeth, Oando George, Hardy Diggory, Drakeley Chris, Smith Thomas A, Cox Jonathan, and Chitnis Nakul
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Simulation ,Kenya ,EIR ,Mathematical Modelling ,Sensitivity analysis ,Malaria ,OpenMalaria ,Arctic medicine. Tropical medicine ,RC955-962 ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Models of Plasmodium falciparum malaria epidemiology that provide realistic quantitative predictions of likely epidemiological outcomes of existing vector control strategies have the potential to assist in planning for the control and elimination of malaria. This work investigates the applicability of mathematical modelling of malaria transmission dynamics in Rachuonyo South, a district with low, unstable transmission in the highlands of western Kenya. Methods Individual-based stochastic simulation models of malaria in humans and a deterministic model of malaria in mosquitoes as part of the OpenMalaria platform were parameterized to create a scenario for the study area based on data from ongoing field studies and available literature. The scenario was simulated for a period of two years with a population of 10,000 individuals and validated against malaria survey data from Rachuonyo South. Simulations were repeated with multiple random seeds and an ensemble of 14 model variants to address stochasticity and model uncertainty. A one-dimensional sensitivity analysis was conducted to address parameter uncertainty. Results The scenario was able to reproduce the seasonal pattern of the entomological inoculation rate (EIR) and patent infections observed in an all-age cohort of individuals sampled monthly for one year. Using an EIR estimated from serology to parameterize the scenario resulted in a closer fit to parasite prevalence than an EIR estimated using entomological methods. The scenario parameterization was most sensitive to changes in the timing and effectiveness of indoor residual spraying (IRS) and the method used to detect P. falciparum in humans. It was less sensitive than expected to changes in vector biting behaviour and climatic patterns. Conclusions The OpenMalaria model of P. falciparum transmission can be used to simulate the impact of different combinations of current and potential control interventions to help plan malaria control in this low transmission setting. In this setting and for these scenarios, results were highly sensitive to transmission, vector exophagy, exophily and susceptibility to IRS, and the detection method used for surveillance. The level of accuracy of the results will thus depend upon the precision of estimates for each. New methods for analysing and evaluating uncertainty in simulation results will enhance the usefulness of simulations for malaria control decision-making. Improved measurement tools and increased primary data collection will enhance model parameterization and epidemiological monitoring. Further research is needed on the relationship between malaria indices to identify the best way to quantify transmission in low transmission settings. Measuring EIR through mosquito collection may not be the optimal way to estimate transmission intensity in areas with low, unstable transmission.
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- 2012
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30. Rationale for short course primaquine in Africa to interrupt malaria transmission
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Eziefula Alice C, Gosling Roly, Hwang Jimee, Hsiang Michelle S, Bousema Teun, von Seidlein Lorenz, and Drakeley Chris
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Plasmodium falciparum ,Malaria ,Primaquine ,8-aminoquinoline ,Transmission ,Gametocyte ,Glucose-6-phosphate dehydrogenase deficiency ,G6PD ,Africa ,Arctic medicine. Tropical medicine ,RC955-962 ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Following the recent successes of malaria control in sub-Saharan Africa, the gametocytocidal drug primaquine needs evaluation as a tool to further reduce the transmission of Plasmodium falciparum malaria. The drug has scarcely been used in Africa because of concerns about its safety in people with glucose-6-phosphate dehydrogenase (G6PD) deficiency. The evidence base for the use of primaquine as a transmission blocker is limited by a lack of comparable clinical and parasitological endpoints between trials. In March 2012, a group of experts met in London to discuss the existing evidence on the ability of primaquine to block malaria transmission, to define the roadblocks to the use of primaquine in Africa and to develop a roadmap to enable its rapid, safe and effective deployment. The output of this meeting is a strategic plan to optimize trial design to reach desired goals efficiently. The roadmap includes suggestions for a series of phase 1, 2, 3 and 4 studies to address specific hurdles to primaquine’s deployment. These include ex-vivo studies on efficacy, primaquine pharmacokinetics and pharmacodynamics and dose escalation studies for safety in high-risk groups. Phase 3 community trials are proposed, along with Phase 4 studies to evaluate safety, particularly in pregnancy, through pharmacovigilance in areas where primaquine is already deployed. In parallel, efforts need to be made to address issues in drug supply and regulation, to map G6PD deficiency and to support the evaluation of alternative gametocytocidal compounds.
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- 2012
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31. Filter paper collection of Plasmodium falciparum mRNA for detecting low-density gametocytes
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Jones Sophie, Sutherland Colin J, Hermsen Cornelus, Arens Theo, Teelen Karina, Hallett Rachel, Corran Patrick, van der Vegte-Bolmer Marga, Sauerwein Robert, Drakeley Chris J, and Bousema Teun
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Reverse-transcription polymerase chain reaction (RT-PCR) ,Quantitative nucleic acid sequence-based amplification (QT-NASBA) ,Sub-microscopic ,Gametocyte ,Detection ,Elimination ,Transmission ,Ribonucleic acid (RNA) ,Filter paper ,Arctic medicine. Tropical medicine ,RC955-962 ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Accurate sampling of sub-microscopic gametocytes is necessary for epidemiological studies to identify the infectious reservoir of Plasmodium falciparum. Detection of gametocyte mRNA achieves sensitive detection, but requires careful handling of samples. Filter papers can be used for collecting RNA samples, but rigorous testing of their capacity to withstand adverse storage conditions has not been fully explored. Methods Three gametocyte dilutions: 10/μL, 1.0/μL and 0.1/μL were spotted onto Whatman™ 903 Protein Saver Cards, FTA Classic Cards and 3MM filter papers that were stored under frozen, cold chain or tropical conditions for up to 13 weeks . RNA was extracted, then detected by quantitative nucleic acid sequence-based amplification (QT-NASBA) and reverse-transcriptase PCR (RT-PCR). Results Successful gametocyte detection was more frequently observed from the Whatman 903 Protein Saver Card compared to the Whatman FTA Classic Card, by both techniques (p Conclusions This study indicates the Whatman 903 Protein Saver Card is better for Pfs25 mRNA sampling compared to the Whatman FTA Classic Card, and that the Whatman 3MM filter paper may prove to be a satisfactory cheaper option for Pfs25 mRNA sampling. When appropriately dried, filter papers provide a useful approach to Pfs25 mRNA sampling, especially in settings where storage in RNA-protecting buffer is not possible.
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- 2012
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32. The biology of sexual development of Plasmodium: the design and implementation of transmission-blocking strategies
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Sinden Robert E, Carter Richard, Drakeley Chris, and Leroy Didier
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Arctic medicine. Tropical medicine ,RC955-962 ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract A meeting to discuss the latest developments in the biology of sexual development of Plasmodium and transmission-control was held April 5-6, 2011, in Bethesda, MD. The meeting was sponsored by the Bill & Melinda Gates Foundation and the National Institutes of Health, National Institute of Allergy and Infectious Diseases (NIH/NIAID) in response to the challenge issued at the Malaria Forum in October 2007 that the malaria community should re-engage with the objective of global eradication. The consequent rebalancing of research priorities has brought to the forefront of the research agenda the essential need to reduce parasite transmission. A key component of any transmission reduction strategy must be methods to attack the parasite as it passes from man to the mosquito (and vice versa). Such methods must be rationally based on a secure understanding of transmission from the molecular-, cellular-, population- to the evolutionary-levels. The meeting represented a first attempt to draw together scientists with expertise in these multiple layers of understanding to discuss the scientific foundations and resources that will be required to provide secure progress toward the design and successful implementation of effective interventions.
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- 2012
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33. Association of sub-microscopic malaria parasite carriage with transmission intensity in north-eastern Tanzania
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Manjurano Alphaxard, Okell Lucy, Lukindo Tedson, Reyburn Hugh, Olomi Raimos, Roper Cally, Clark Taane G, Joseph Sarah, Riley Eleanor M, and Drakeley Chris
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Arctic medicine. Tropical medicine ,RC955-962 ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background In malaria endemic areas, individuals are frequently asymptomatic and may be undetected by conventional microscopy or newer, rapid diagnostic tests. Molecular techniques allow a more accurate assessment of this asymptomatic parasite burden, the extent of which is important for malaria control. This study examines the relative prevalence of sub-microscopic level parasite carriage and clonal complexity of infections (multiplicity of infection) over a range of endemicities in a region of north-eastern Tanzania where altitude is an established proxy of malaria transmission. The PCR prevalence was then compared against other measures of transmission intensity collected in the same area. Methods This study used 1,121 blood samples collected from a previously conducted cross-sectional malario-metric survey during the short rainy season in 2001 from 13 villages (three at < 600 m, four at 600-1,200 m and six at > 1,200 m in altitude above sea level). Samples were analysed by PCR for carriage of parasites and multiplicity of infection. These data were compared with other measures of transmission intensity collected from the same area. Results Parasite prevalence was 34.7% by PCR and 13.6% by microscopy; a 2.5-fold difference in line with other recent observations. This fold difference was relatively consistent at the different altitude bands despite a marked decrease in parasite prevalence with altitude: < 600 m 70.9 vs 28.6, 600-1,200 m 35.5 vs 9.9, > 1,200 m 15.8 vs 5.9. The difference between parasite prevalence by PCR was 3.2 in individuals aged between 15 and 45 years (34.5 vs 10.9) compared with 2.5 in those aged 1-5 (34.0 vs 13.5) though this was not statistically significant. Multiplicity of infection (MOI) ranged from 1.2 to 3.7 and was positively associated with parasite prevalence assessed by both PCR and microscopy. There was no association of MOI and age. Village level PCR parasite prevalence was strongly correlated with altitude, sero-conversion rate and predicted entomological inoculation rate. Conclusions Asymptomatic, low density, multi-clone malaria infection was common in this study area. These infections are important as potential contributors to the infectious reservoir of parasites and need to be identified by control programmes especially in this era where malaria elimination is a focus. High throughput standardized PCR approaches are needed to identify individuals who are malaria carriers.
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- 2011
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34. A cluster-randomized trial of mass drug administration with a gametocytocidal drug combination to interrupt malaria transmission in a low endemic area in Tanzania
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Shekalaghe Seif A, Drakeley Chris, van den Bosch Sven, ter Braak Roel, van den Bijllaardt Wouter, Mwanziva Charles, Semvua Salimu, Masokoto Alutu, Mosha Frank, Teelen Karina, Hermsen Rob, Okell Lucy, Gosling Roly, Sauerwein Robert, and Bousema Teun
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Arctic medicine. Tropical medicine ,RC955-962 ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Effective mass drug administration (MDA) with anti-malarial drugs can clear the human infectious reservoir for malaria and thereby interrupt malaria transmission. The likelihood of success of MDA depends on the intensity and seasonality of malaria transmission, the efficacy of the intervention in rapidly clearing all malaria parasite stages and the degree to which symptomatic and asymptomatic parasite carriers participate in the intervention. The impact of MDA with the gametocytocidal drug combination sulphadoxine-pyrimethamine (SP) plus artesunate (AS) plus primaquine (PQ, single dose 0.75 mg/kg) on malaria transmission was determined in an area of very low and seasonal malaria transmission in northern Tanzania. Methods In a cluster-randomized trial in four villages in Lower Moshi, Tanzania, eight clusters (1,110 individuals; cluster size 47- 209) were randomized to observed treatment with SP+AS+PQ and eight clusters (2,347 individuals, cluster size 55- 737) to treatment with placebo over three days. Intervention and control clusters were 1km apart; households that were located between clusters were treated as buffer zones where all individuals received SP+AS+PQ but were not selected for the evaluation. Passive case detection was done for the entire cohort and active case detection in 149 children aged 1-10 year from the intervention arm and 143 from the control arm. Four cross-sectional surveys assessed parasite carriage by microscopy and molecular methods during a five-month follow-up period. Results The coverage rate in the intervention arm was 93.0% (1,117/1,201). Parasite prevalence by molecular detection methods was 2.2-2.7% prior to the intervention and undetectable during follow-up in both the control and intervention clusters. None of the slides collected during cross-sectional surveys had microscopically detectable parasite densities. Three clinical malaria episodes occurred in the intervention (n = 1) and control clusters (n = 2). Conclusions This study illustrates the possibility to achieve high coverage with a three-day intervention but also the difficulty in defining suitable outcome measures to evaluate interventions in areas of very low malaria transmission intensity. The decline in transmission intensity prior to the intervention made it impossible to assess the impact of MDA in the chosen study setting. Trial Registration ClinicalTrials.gov: NCT00509015
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- 2011
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35. Development of standardized laboratory methods and quality processes for a phase III study of the RTS, S/AS01 candidate malaria vaccine
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Carter Terrell, Villafana Tonya, Okech Brenda, Ofori-Anyinam Opokua, Greenwood Brian, Kremsner Peter, Drakeley Chris, Oyakhirome Sunny, Bruls Myriam, Vekemans Johan, Swysen Christine, Savarese Barbara, Duse Adriano, Reijman Andrea, Ingram Charlotte, Frean John, and Ogutu Bernhards
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Arctic medicine. Tropical medicine ,RC955-962 ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background A pivotal phase III study of the RTS,S/AS01 malaria candidate vaccine is ongoing in several research centres across Africa. The development and establishment of quality systems was a requirement for trial conduct to meet international regulatory standards, as well as providing an important capacity strengthening opportunity for study centres. Methods Standardized laboratory methods and quality assurance processes were implemented at each of the study centres, facilitated by funding partners. Results A robust protocol for determination of parasite density based on actual blood cell counts was set up in accordance with World Health Organization recommendations. Automated equipment including haematology and biochemistry analyzers were put in place with standard methods for bedside testing of glycaemia, base excess and lactacidaemia. Facilities for X-rays and basic microbiology testing were also provided or upgraded alongside health care infrastructure in some centres. External quality assurance assessment of all major laboratory methods was established and method qualification by each laboratory demonstrated. The resulting capacity strengthening has ensured laboratory evaluations are conducted locally to the high standards required in clinical trials. Conclusion Major efforts by study centres, together with support from collaborating parties, have allowed standardized methods and robust quality assurance processes to be put in place for the phase III evaluation of the RTS, S/AS01 malaria candidate vaccine. Extensive training programmes, coupled with continuous commitment from research centre staff, have been the key elements behind the successful implementation of quality processes. It is expected these activities will culminate in healthcare benefits for the subjects and communities participating in these trials. Trial registration Clinicaltrials.gov NCT00866619
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- 2011
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36. Human saliva as a source of anti-malarial antibodies to examine population exposure to Plasmodium falciparum
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West Sheila, Nwakanma Davis C, Satoguina Judith, Estévez Patricia, Conway David J, and Drakeley Chris J
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Arctic medicine. Tropical medicine ,RC955-962 ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Antibody responses to malaria antigens reflect exposure to parasites, and seroprevalence correlates with malaria transmission intensity. Antibodies are routinely measured in sera or on dried blood spots but a non-invasive method would provide extra utility in sampling general populations. Saliva is already in use in the detection of plasma-derived IgM and IgG to viral infections. In this study, antibodies to Plasmodium falciparum merozoite antigens were compared between blood and saliva samples from the same individuals in unlinked surveys conducted in Tanzania and The Gambia. Methods In Tanzania, 53 individuals provided paired fingerprick blood and saliva sample using two commercially available sampling devices. In the Gambia, archived plasma and saliva samples collected from 200 children in the Farafenni area in a cross-sectional survey were analyzed. IgG antibodies against P. falciparum antigens, Merozoite Surface Protein-1 (MSP-119) and Apical membrane Antigen (AMA-1) were measured by ELISA in paired saliva and blood samples from both sites. Antibody levels were compared as continuous optical density (OD) values and by sero-positivity. Results Significant correlations between saliva and plasma antibody levels were seen in Tanzania for both antigens, AMA-1(r2 range 0.93 to 0.89, p < 0.001) and MSP-119 (r2 range 0.93 to 0.75, p < 0.001), with a weaker correlation for results from The Gambia (r2range 0.64 to 0.63, p < 0.01). When assessed as seropositivity and compared with plasma, sensitivity and specificity were good with saliva antibody levels to both AMA-1 and MSP-119 (sensitivity range 64-77% and specificity range 91-100% & 47-67% and 90-97% respectively) over the different sample sets. Conclusions These data demonstrate anti-malarial antibodies can be detected in saliva and correlate strongly with levels in plasma. This non-invasive relatively simple collection method will be potentially useful for general population surveys, and particularly in migratory populations or those with infrequent contact with health services or opposed to blood withdrawal. Further studies will be needed to optimize collection methods, standardize volumes and content and develop controls.
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- 2011
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37. The plasticity of Plasmodium falciparum gametocytaemia in relation to age in Burkina Faso
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Drakeley Chris, Verhave Jan-Peter, Cuzin-Ouattara Nadine, de Vlas Sake J, Bousema Teun, Ouédraogo André, Luty Adrian JF, and Sauerwein Robert
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Arctic medicine. Tropical medicine ,RC955-962 ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Malaria transmission depends on the presence of gametocytes in the peripheral blood. In this study, the age-dependency of gametocytaemia was examined by microscopy and molecular tools. Methods A total of 5,383 blood samples from individuals of all ages were collected over six cross sectional surveys in Burkina Faso. One cross-sectional study used quantitative nucleic acid sequence based amplification (QT-NASBA) for parasite quantification (n = 412). The proportion of infections with concurrent gametocytaemia and median proportion of gametocytes among all parasites were calculated. Results Asexual parasite prevalence and gametocyte prevalence decreased with age. Gametocytes made up 1.8% of the total parasite population detected by microscopy in the youngest age group. This proportion gradually increased to 18.2% in adults (p < 0.001). Similarly, gametocytes made up 0.2% of the total parasite population detected by QT-NASBA in the youngest age group, increasing to 5.7% in adults (p < 0.001). This age pattern in gametocytaemia was also evident in the proportion of gametocyte positive slides without concomitant asexual parasites which increased from 13.4% (17/127) in children to 45.6% (52/114) in adults (OR 1.55, 95% CI 1.38-1.74, p < 0.001). Conclusions The findings of this study suggest that although gametocytes are most commonly detected in children, the proportion of asexual parasites that is committed to develop into gametocytes may increase with age. These findings underscore the importance of adults for the human infectious reservoir for malaria.
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- 2010
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38. Using serological measures to monitor changes in malaria transmission in Vanuatu
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Clements Archie, Taleo George, Kuwahata Melissa, Iavro Jennifer, Reid Heidi, Cook Jackie, McCarthy James, Vallely Andrew, and Drakeley Chris
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Arctic medicine. Tropical medicine ,RC955-962 ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background With renewed interest in malaria elimination, island environments present unique opportunities to achieve this goal. However, as transmission decreases, monitoring and evaluation programmes need increasingly sensitive tools to assess Plasmodium falciparum and Plasmodium vivax exposure. In 2009, to assess the role of serological markers in evaluating malaria transmission, a cross-sectional seroprevalence study was carried out in Tanna and Aneityum, two of the southernmost islands of the Vanuatu archipelago, areas where malaria transmission has been variably reduced over the past few decades. Methods Malaria transmission was assessed using serological markers for exposure to P. falciparum and P. vivax. Filter blood spot papers were collected from 1,249 people from Tanna, and 517 people from Aneityum to assess the prevalence of antibodies to two P. falciparum antigens (MSP-119 and AMA-1) and two P. vivax antigens (MSP-119 and AMA-1). Age-specific prevalence was modelled using a simple catalytic conversion model based on maximum likelihood to generate a community seroconversion rate (SCR). Results Overall seropositivity in Tanna was 9.4%, 12.4% and 16.6% to P. falciparum MSP-119, AMA-1 and Schizont Extract respectively and 12.6% and 15.0% to P. vivax MSP-119 and AMA-1 respectively. Serological results distinguished between areas of differential dominance of either P. vivax or P. falciparum and analysis of age-stratified results showed a step in seroprevalence occurring approximately 30 years ago on both islands, indicative of a change in transmission intensity at this time. Results from Aneityum suggest that several children may have been exposed to malaria since the 2002 P. vivax epidemic. Conclusion Seroepidemiology can provide key information on malaria transmission for control programmes, when parasite rates are low. As Vanuatu moves closer to malaria elimination, monitoring changes in transmission intensity and identification of residual malaria foci is paramount in order to concentrate intervention efforts.
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- 2010
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39. Revisiting the circulation time of Plasmodium falciparum gametocytes: molecular detection methods to estimate the duration of gametocyte carriage and the effect of gametocytocidal drugs
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Sawa Patrick, Omar Sabah, Griffin Jamie T, Shekalaghe Seif, Okell Lucy, Bousema Teun, Sutherland Colin, Sauerwein Robert, Ghani Azra C, and Drakeley Chris
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Arctic medicine. Tropical medicine ,RC955-962 ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background There is renewed acknowledgement that targeting gametocytes is essential for malaria control and elimination efforts. Simple mathematical models were fitted to data from clinical trials in order to determine the mean gametocyte circulation time and duration of gametocyte carriage in treated malaria patients. Methods Data were used from clinical trials from East Africa. The first trial compared non-artemisinin combination therapy (non-ACT: sulphadoxine-pyrimethamine (SP) plus amodiaquine) and artemisinin-based combination therapy (ACT: SP plus artesunate (AS) or artemether-lumefantrine). The second trial compared ACT (SP+AS) with ACT in combination with a single dose of primaquine (ACT-PQ: SP+AS+PQ). Mature gametocytes were quantified in peripheral blood samples by nucleic acid sequence based amplification. A simple deterministic compartmental model was fitted to gametocyte densities to estimate the circulation time per gametocyte; a similar model was fitted to gametocyte prevalences to estimate the duration of gametocyte carriage after efficacious treatment. Results The mean circulation time of gametocytes was 4.6-6.5 days. After non-ACT treatment, patients were estimated to carry gametocytes for an average of 55 days (95% CI 28.7 - 107.7). ACT reduced the duration of gametocyte carriage fourfold to 13.4 days (95% CI 10.2-17.5). Addition of PQ to ACT resulted in a further fourfold reduction of the duration of gametocyte carriage. Conclusions These findings confirm previous estimates of the circulation time of gametocytes, but indicate a much longer duration of (low density) gametocyte carriage after apparently successful clearance of asexual parasites. ACT shortened the period of gametocyte carriage considerably, and had the most pronounced effect on mature gametocytes when combined with PQ.
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- 2010
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40. Heterogeneity in malaria exposure and vaccine response: implications for the interpretation of vaccine efficacy trials
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Drakeley Chris J, Griffin Jamie T, White Michael T, and Ghani Azra C
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Arctic medicine. Tropical medicine ,RC955-962 ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Phase III trials of the malaria vaccine, RTS, S, are now underway across multiple sites of varying transmission intensity in Africa. Heterogeneity in exposure, vaccine response and waning of efficacy may bias estimates of vaccine efficacy. Methods Theoretical arguments are used to identify the expected effects of a) heterogeneity in exposure to infectious bites; b) heterogeneity in individual's response to the vaccine; and c) waning efficacy on measures of vaccine efficacy from clinical trials for an infection-blocking vaccine. Results Heterogeneity in exposure and vaccine response leads to a smaller proportion of trial participants becoming infected than one would expect in a homogeneous setting. This causes estimates of vaccine efficacy from clinical trials to be underestimated if transmission heterogeneity is ignored, and overestimated if heterogeneity in vaccine response is ignored. Waning of vaccine efficacy can bias estimates of vaccine efficacy in both directions. Conclusions Failure to account for heterogeneities in exposure and response, and waning of efficacy in clinical trials can lead to biased estimates of malaria vaccine efficacy. Appropriate methods to reduce these biases need to be used to ensure accurate interpretation and comparability between trial sites of results from the upcoming Phase III clinical trials of RTS, S.
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- 2010
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41. Assessing agreement between malaria slide density readings
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Ngasala Billy, Schellenberg David, Alexander Neal, Petzold Max, Drakeley Chris, and Sutherland Colin
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Arctic medicine. Tropical medicine ,RC955-962 ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Several criteria have been used to assess agreement between replicate slide readings of malaria parasite density. Such criteria may be based on percent difference, or absolute difference, or a combination. Neither the rationale for choosing between these types of criteria, nor that for choosing the magnitude of difference which defines acceptable agreement, are clear. The current paper seeks a procedure which avoids the disadvantages of these current options and whose parameter values are more clearly justified. Methods and Results Variation of parasite density within a slide is expected, even when it has been prepared from a homogeneous sample. This places lower limits on sensitivity and observer agreement, quantified by the Poisson distribution. This means that, if a criterion of fixed percent difference criterion is used for satisfactory agreement, the number of discrepant readings is over-estimated at low parasite densities. With a criterion of fixed absolute difference, the same happens at high parasite densities. For an ideal slide, following the Poisson distribution, a criterion based on a constant difference in square root counts would apply for all densities. This can be back-transformed to a difference in absolute counts, which, as expected, gives a wider range of acceptable agreement at higher average densities. In an example dataset from Tanzania, observed differences in square root counts correspond to a 95% limits of agreement of -2,800 and +2,500 parasites/μl at average density of 2,000 parasites/μl, and -6,200 and +5,700 parasites/μl at 10,000 parasites/μl. However, there were more outliers beyond those ranges at higher densities, meaning that actual coverage of these ranges was not a constant 95%, but decreased with density. In a second study, a trial of microscopist training, the corresponding ranges of agreement are wider and asymmetrical: -8,600 to +5,200/μl, and -19,200 to +11,700/μl, respectively. By comparison, the optimal limits of agreement, corresponding to Poisson variation, are ± 780 and ± 1,800 parasites/μl, respectively. The focus of this approach on the volume of blood read leads to other conclusions. For example, no matter how large a volume of blood is read, some densities are too low to be reliably detected, which in turn means that disagreements on slide positivity may simply result from within-slide variation, rather than reading errors. Conclusions The proposed method defines limits of acceptable agreement in a way which allows for the natural increase in variability with parasite density. This includes defining the levels of between-reader variability, which are consistent with random variation: disagreements within these limits should not trigger additional readings. This approach merits investigation in other settings, in order to determine both the extent of its applicability, and appropriate numerical values for limits of agreement.
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- 2010
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42. Immunophoretic rapid diagnostic tests as a source of immunoglobulins for estimating malaria sero-prevalence and transmission intensity
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Reyburn Hugh, Mtove George, Stewart Laveta, Mweya Clement, Williams Geoffrey S, Cook Jackie, Corran Patrick H, Riley Eleanor M, and Drakeley Chris J
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Arctic medicine. Tropical medicine ,RC955-962 ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Sero-epidemiological methods are being developed as a tool for rapid assessment of malaria transmission intensity. Simple blood collection methods for use in field settings will make this more feasible. This paper describes validation of such a method, by analysing immunoglobulins from blood retained within immunophoretic rapid diagnostic tests (RDTs) for Plasmodium falciparum. RDTs are now widely used for the diagnosis of malaria and estimation of parasite rates, and this method represents a further use for these devices in malaria control. Methods Immunoglobulins eluted from RDTs, designed to detect parasite histidine rich protein-2 (HRP-2), were analysed by indirect ELISA for IgG recognizing the P. falciparum blood stage antigens merozoite surface protein-119 (MSP-119) and apical membrane antigen-1 (AMA-1). Optimal storage conditions for RDTs were evaluated by comparing antibody responses from RDTs stored in dry or humid conditions at 4°C or at ambient temperature (with or without air-conditioning) for 7, 31 or 70 days. Antibody levels estimated using 3,700 RDT samples from attendees at health facilities in North-eastern Tanzania were compared with contemporaneously collected filter paper blood spots (FPBS) and used to estimate seroconversion rates. Results Storage of RDTs at 4°C was optimal for immunoglobulin recovery but short-term storage at ambient temperatures did not substantially affect anti-malarial IgG levels. Results from RDTs were comparable with those from FPBSs, for both antigens. RDT-generated titres tended to be slightly higher than those generated from FPBSs, possibly due to greater recovery of immunoglobulins from RDTs compared to filter paper. Importantly, however, RDT-based seroconversion rates, and hence serological estimates of malaria transmission intensity, agreed closely with those from FPBSs. Conclusion RDTs represent a practical option for collecting blood for sero-epidemiological surveys, with potential cost and logistical advantages over filter paper and other blood collection methods. RDT-based seroepidemiology can be incorporated into routine monitoring of malaria endemicity, providing information to supplement parasite prevalence rates and generating rapid, robust assessment of malaria transmission intensity at minimal extra cost.
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- 2009
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43. Low density parasitaemia, red blood cell polymorphisms and Plasmodium falciparum specific immune responses in a low endemic area in northern Tanzania
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Sauerwein Robert, Kavishe Reginald, Manjurano Alphaxard, Mkali Humphrey, Mwakalinga Steve, Enevold Anders, Mwanziva Charles, Alifrangis Michael, Shekalaghe Seif, Drakeley Chris, and Bousema Teun
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Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Low density Plasmodium falciparum infections, below the microscopic detection limit, may play an important role in maintaining malaria transmission in low endemic areas as well as contribute to the maintenance of acquired immunity. Little is known about factors influencing the occurrence of sub-microscopic parasitaemia or the relation with immune responses. We investigated possible associations between the occurrence of sub-microscopic P. falciparum parasite carriage and antibody responses to the asexual stage antigens, G6PD deficiency and α+-thalassaemia in 464 subjects from a low endemic area in northern Tanzania. Methods We used samples collected from two cross sectional surveys conducted during dry and wet season in 2005. Submicroscopic parasitaemia was detected by using quantitative nucleic acid sequence based amplification (QT-NASBA). Genotyping for G6PD and α+-thalassaemia were performed by high throughput PCR; the prevalence and level of total IgG antibodies against MSP-1, MSP-2 and AMA-1 were determined by ELISA. Results Compared to parasite free individuals, individuals carrying sub-microscopic densities of P. falciparum parasites had significantly higher median antibody levels to MSP-1 (p = 0.042) and MSP-2 (p = 0.034) but not to AMA-1 (p = 0.14) while no clear relation between sub-microscopic parasite carriage and G6PD deficiency or α+-thalassaemia was observed. Conclusion Our data suggest a role for sub-microscopic parasite densities in eliciting or maintaining humoral immune responses without evidence for a modulating effect of G6PD deficiency or α+-thalassaemia.
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- 2009
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44. Analytical approaches for antimalarial antibody responses to confirm historical and recent malaria transmission: an example from the Philippines
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Macalinao, Maria Lourdes M., Fornace, Kimberly M., Reyes, Ralph A., Hall, Tom, Bareng, Alison Paolo N., Adams, John H., Huon, Christèle, Chitnis, Chetan E., Luchavez, Jennifer S., Tetteh, Kevin K.A., Yui, Katsuyuki, Hafalla, Julius Clemence R., Espino, Fe Esperanza J., and Drakeley, Chris J.
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- 2023
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45. Plasmodium falciparum gametocyte dynamics in areas of different malaria endemicity
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von Seidlein Lorenz, Drakeley Chris J, Sutherland Colin J, Price Ric N, Stepniewska Kasia, Nosten Francois, and White Nicholas J
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Arctic medicine. Tropical medicine ,RC955-962 ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background The aim of this study was to identify and compare factors associated with Plasmodium falciparum gametocyte carriage in three regions of differing malaria endemicity. Methods Retrospective data from Thailand, The Gambia and Tanzania were used. The data came from large prospective field-based clinical trials, which investigated gametocyte carriage after different anti-malarial drug treatments. Results Gametocytaemia was detected during the observation period in 12% of patients (931 out of 7548) in Thailand, 34% (683 out of 2020) in The Gambia, and 31% (430 out of 1400) in Tanzania (p < 0.001). Approximately one third (33%, 680/2044) of the patients with gametocytaemia during the observation period, already had patent gametocytaemia at enrolment (day 0 or day 1): 35% (318/931) in Thailand, 37% (250/683) in The Gambia, 26% (112/430) in Tanzania. Maximum gametocytaemia was usually observed on or before the seventh day after starting treatment (93% in Thailand, 70% in Tanzania and 78% in The Gambia). Lowest gametocyte carriage rates were observed following treatment with artemisinin derivatives, while sulphadoxine-pyrimethamine (SP) was associated with significantly greater development of gametocytaemia than other drug treatments (p < 0.001). The duration of gametocyte carriage was shorter in Thailand by 86% and Tanzania by 65% than in The Gambia. Gametocyte carriage was 27% longer among people presenting with anaemia, and was shorter in duration among patients who received artemisinin derivatives, by 27% in Thailand and by 71% in Tanzania and The Gambia. Conclusion This study confirms the independent association of gametocytaemia with anaemia, and the significantly lower prevalence and duration of gametocyte carriage following treatment with an artemisinin derivative. The large differences in gametocyte carriage rates between regions with different levels of malaria transmission suggest that drug interventions to prevent transmission will have different effects in different places.
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- 2008
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46. Overuse of artemisinin-combination therapy in Mto wa Mbu (river of mosquitoes), an area misinterpreted as high endemic for malaria
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Sauerwein Robert, Mosha Frank, Megiroo Simon, Mengerink Bianca, Ndaro Arnold, Shekalaghe Seif, Mwanziva Charles, Drakeley Chris, Gosling Roly, and Bousema Teun
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Arctic medicine. Tropical medicine ,RC955-962 ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Adequate malaria diagnosis and treatment remain major difficulties in rural sub-Saharan Africa. These issues deserve renewed attention in the light of first-line treatment with expensive artemisinin-combination therapy (ACT) and changing patterns of transmission intensity. This study describes diagnostic and treatment practices in Mto wa Mbu, an area that used to be hyperendemic for malaria, but where no recent assessments of transmission intensity have been conducted. Methods Retrospective and prospective data were collected from the two major village health clinics. The diagnosis in prospectively collected data was confirmed by microscopy. The level of transmission intensity was determined by entomological assessment and by estimating sero-conversion rates using anti-malarial antibody responses. Results Malaria transmission intensity by serological assessment was equivalent to < 1 infectious bites per person per year. Despite low transmission intensity, > 40% of outpatients attending the clinics in 2006–2007 were diagnosed with malaria. Prospective data demonstrated a very high overdiagnosis of malaria. Microscopy was unreliable with < 1% of slides regarded as malaria parasite-positive by clinic microscopists being confirmed by trained research microscopists. In addition, many 'slide negatives' received anti-malarial treatment. As a result, 99.6% (248/249) of the individuals who were treated with ACT were in fact free of malaria parasites. Conclusion Transmission intensity has dropped considerably in the area of Mto wa Mbu. Despite this, most fevers are still regarded and treated as malaria, thereby ignoring true causes of febrile illness and over-prescribing ACT. The discrepancy between the perceived and actual level of transmission intensity may be present in many areas in sub-Saharan Africa and calls for greater efforts in defining levels of transmission on a local scale to help rational drug-prescribing behaviour.
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- 2008
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47. Dried blood spots as a source of anti-malarial antibodies for epidemiological studies
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Abeku Tarekegn, Cox Jonathan, Griffin Jamie, Manjurano Alphaxard, Leendertse Heleen, Lynch Caroline, Cook Jackie, Corran Patrick H, Bousema Teun, Ghani Azra C, Drakeley Chris, and Riley Eleanor
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Arctic medicine. Tropical medicine ,RC955-962 ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Blood spots collected onto filter paper are an established and convenient source of antibodies for serological diagnosis and epidemiological surveys. Although recommendations for the storage and analysis of small molecule analytes in blood spots exist, there are no published systematic studies of the stability of antibodies under different storage conditions. Methods Blood spots, on filter paper or glass fibre mats and containing malaria-endemic plasma, were desiccated and stored at various temperatures for different times. Eluates of these spots were assayed for antibodies against two Plasmodium falciparum antigens, MSP-119 and MSP2, and calculated titres used to fit an exponential (first order kinetic) decay model. The first order rate constants (k) for each spot storage temperature were used to fit an Arrhenius equation, in order to estimate the thermal and temporal stability of antibodies in dried blood spots. The utility of blood spots for serological assays was confirmed by comparing antibodies eluted from blood spots with the equivalent plasma values in a series of samples from North Eastern Tanzania and by using blood spot-derived antibodies to estimate malaria transmission intensity in this site and for two localities in Uganda. Results Antibodies in spots on filter paper and glass fibre paper had similar stabilities but blood was more easily absorbed onto filter papers than glass fibre, spots were more regular and spot size was more closely correlated with blood volume for filter paper spots. Desiccated spots could be stored at or below 4°C for extended periods, but were stable for only very limited periods at ambient temperature. When desiccated, recoveries of antibodies that are predominantly of IgG1 or IgG3 subclasses were similar. Recoveries of antibodies from paired samples of serum and of blood spots from Tanzania which had been suitably stored showed similar recoveries of antibodies, but spots which had been stored for extended periods at ambient humidity and temperature showed severe loss of recoveries. Estimates of malaria transmission intensity obtained from serum and from blood spots were similar, and values obtained using blood spots agreed well with entomologically determined values. Conclusion This study has demonstrated the suitability of filter paper blood spots paper for collection of serum antibodies, and provided clear guidelines for the treatment and storage of filter papers which emphasize the importance of desiccation and minimisation of time spent at ambient temperatures. A recommended protocol for collecting, storing and assaying blood spots is provided.
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- 2008
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48. Dry season ecology of Anopheles gambiae complex mosquitoes in The Gambia
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Bogh Claus, Jallow Ebrima, Nwakanma Davis C, Drakeley Chris J, Pinder Margaret, Jawara Musa, Lindsay Steve W, and Conway David J
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Arctic medicine. Tropical medicine ,RC955-962 ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Malaria in The Gambia is highly seasonal, with transmission occurring as Anopheles gambiae s.l. populations expand during and immediately after a single annual rainy season that lasts from June to October. There has been very limited investigation of the ecology of vectors during the dry season, when numbers are very limited and distributions may be restricted. Methods Weekly adult mosquito collections (pyrethrum spray, light trap, and search collections from rooms, as well as light trap collections from animal shelters, abandoned wells and grain stores), and artificial sentinel breeding site surveys were performed in four villages near the upper tidal and partially saline part of the Gambia River in the last four months of an annual dry season (March to June). Mosquito species were identified by morphological and DNA analysis, and ELISA assays were performed to test for Plasmodium falciparum sporozoites and human blood meal components. Results Adults of An. gambiae s.l. were collected throughout the period, numbers increasing towards the end of the dry season when humidity was increasing. Adult collections were dominated by An. melas (86%), with An. gambiae s.s. (10%) and An. arabiensis (3%) also present throughout. Most females collected in room search and spray collections contained blood meals, but most from light traps were unfed. None of the females tested (n = 1709) contained sporozoites. Larvae (mostly An. gambiae s.s.) were recovered from artificial sentinel breeding sites in the two villages that had freshwater pools. These two villages had the highest proportions of An. gambiae s.s. adults, and experienced the most substantial increase in proportions of An. gambiae s.s. after the onset of rains. Conclusion During the dry season population minimum, An. melas was the predominant vector species, but differences among villages in availability of fresh-water breeding sites correlate with egg laying activity and relative numbers of An. gambiae s.s. adults, and with the increase in this species immediately after the beginning of the rains. Local variation in dry season vector persistence is thus likely to influence spatial heterogeneity of transmission intensity in the early part of the rainy season.
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- 2008
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49. Reduction of transmission from malaria patients by artemisinin combination therapies: a pooled analysis of six randomized trials
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Bousema Teun, Ghani Azra C, Drakeley Chris J, Okell Lucy C, and Sutherland Colin J
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Arctic medicine. Tropical medicine ,RC955-962 ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Artemisinin combination therapies (ACT), which are increasingly being introduced for treatment of Plasmodium falciparum malaria, are more effective against sexual stage parasites (gametocytes) than previous first-line antimalarials and therefore have the potential to reduce parasite transmission. The size of this effect is estimated in symptomatic P. falciparum infections. Methods Data on 3,174 patients were pooled from six antimalarial trials conducted in The Gambia and Kenya. Multivariable regression was used to investigate the role of ACT versus non-artemisinin antimalarial treatment, treatment failure, presence of pre-treatment gametocytes and submicroscopic gametocytaemia on transmission to mosquitoes and the area under the curve (AUC) of gametocyte density during the 28 days of follow up. Results ACT treatment was associated with a significant reduction in the probability of being gametocytaemic on the day of transmission experiments (OR 0.20 95% CI 0.16–0.26), transmission to mosquitoes by slide-positive gametocyte carriers (OR mosquito infection 0.49 95% CI 0.33–0.73) and AUC of gametocyte density (ratio of means 0.35 95% CI 0.31–0.41). Parasitological treatment failure did not account for the difference between ACT and non-artemisinin impact. The presence of slide-positive gametocytaemia prior to treatment significantly reduced ACT impact on gametocytaemia (p < 0.001). Taking account of submicroscopic gametocytaemia reduced estimates of ACT impact in a high transmission setting in Kenya, but not in a lower transmission setting in the Gambia. Conclusion Treatment with ACT significantly reduces infectiousness of individual patients with uncomplicated falciparum malaria compared to previous first line treatments. Rapid treatment of cases before gametocytaemia is well developed may enhance the impact of ACT on transmission.
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- 2008
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50. Associations between red blood cell variants and malaria among children and adults from three areas of Uganda: a prospective cohort study
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Kakande, Elijah, Greenhouse, Bryan, Bajunirwe, Francis, Drakeley, Chris, Nankabirwa, Joaniter I, Walakira, Andrew, Nsobya, Samuel L, Katureebe, Agaba, Rek, John, Arinaitwe, Emmanuel, Rosenthal, Philip J, Kamya, Moses R, Dorsey, Grant, and Rodriguez-Barraquer, Isabel
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Medical Microbiology ,Biomedical and Clinical Sciences ,Clinical Sciences ,Rare Diseases ,Pediatric ,Malaria ,Prevention ,Clinical Research ,Infectious Diseases ,Vector-Borne Diseases ,Genetics ,2.1 Biological and endogenous factors ,2.2 Factors relating to the physical environment ,Aetiology ,Infection ,Good Health and Well Being ,Adult ,Age Factors ,Binomial Distribution ,Caregivers ,Child ,Child ,Preschool ,Cohort Studies ,Erythrocytes ,Female ,Humans ,Incidence ,Infant ,Longitudinal Studies ,Male ,Parasitemia ,Prevalence ,Prospective Studies ,Sex Factors ,Uganda ,Young Adult ,Red blood cell variants ,Erythrocyte ,Plasmodium ,Sickle hemoglobin ,Thalassemia ,Microbiology ,Public Health and Health Services ,Tropical Medicine ,Medical microbiology ,Public health - Abstract
BackgroundMultiple red blood cell (RBC) variants appear to offer protection against the most severe forms of Plasmodium falciparum malaria. Associations between these variants and uncomplicated malaria are less clear.MethodsData from a longitudinal cohort study conducted in 3 sub-counties in Uganda was used to quantify associations between three red blood cell variants Hb [AA, AS, S (rs334)], alpha thalassaemia 3.7 kb deletion, and glucose-6-phosphate dehydrogenase deficiency A-(G6PD 202A genotype) and malaria incidence, parasite prevalence, parasite density (a measure of anti-parasite immunity) and body temperature adjusted for parasite density (a measure of anti-disease immunity). All analyses were adjusted for age, average household entomological inoculation rate, and study site. Results for all variants were compared to those for wild type genotypes.ResultsIn children, HbAS was associated, compared to wild type, with a lower incidence of malaria (IRR = 0.78, 95% CI 0.66-0.92, p = 0.003), lower parasite density upon infection (PR = 0.66, 95% CI 0.51-0.85, p = 0.001), and lower body temperature for any given parasite density (- 0.13 ℃, 95% CI - 0.21, - 0.05, p = 0.002). In children, HbSS was associated with a lower incidence of malaria (IRR = 0.17, 95% CI 0.04-0.71, p = 0.02) and lower parasite density upon infection (PR = 0.31, 95% CI 0.18-0.54, p
- Published
- 2020
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