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43 results on '"Dou, Tonghai"'

11. Identification and characterisation of bifidobacteria in infant formula milk powder obtained from the Chinese market

Author

Dou Tonghai, Yu Yi, Weng Shiyu, Liu Yang, Chen Wenwen, Qu Qinfeng, Guoping Zhao, Duan Wenfeng, Zhang Qingping, and Yu Zhao

Subjects
0301 basic medicine, Gel electrophoresis, biology, Strain (biology), 030106 microbiology, 16S ribosomal RNA, biology.organism_classification, Applied Microbiology and Biotechnology, Bifidobacterium animalis, law.invention, 03 medical and health sciences, Probiotic, Infant formula, law, Multilocus sequence typing, Food science, Genotyping, Food Science
Abstract

Strains of Bifidobacterium animalis subsp. lactis (BAL) are the most commonly added probiotic strains in infant formulas on the Chinese market. In this study, four genotyping methods, 16S rRNA sequencing, multilocus sequence typing, pulsed-field gel electrophoresis and single nucleotide polymorphism-insertion deletion polymorphism were used to determine the 30 isolates from 20 commercial products at the species and strain levels. A combination of the label analysis, quantification and identification results showed that the label claims from the 20 samples had an overall accuracy of 80%. This study was the first to use molecular techniques to identify bifidobacteria strains in probiotic infant formulas on the Chinese market. The results demonstrated that the combination of genotyping methods can effectively identify commonly used commercial BAL strains with similar genetic backgrounds and highlight the importance of accurate strain identification by manufacturers to ensure product legitimacy and to avoid potential health risks.

Published
2018

15. 5′ UTR CGG repeat expansion in GIPC1 is associated with oculopharyngodistal myopathy

Author

Xi, Jianying, primary, Wang, Xilu, additional, Yue, Dongyue, additional, Dou, Tonghai, additional, Wu, Qunfeng, additional, Lu, Jun, additional, Liu, Yiqi, additional, Yu, Wenbo, additional, Qiao, Kai, additional, Lin, Jie, additional, Luo, Sushan, additional, Li, Jing, additional, Du, Ailian, additional, Dong, Jihong, additional, Chen, Yan, additional, Luo, Lijun, additional, Yang, Jie, additional, Niu, Zhenmin, additional, Liang, Zonghui, additional, Zhao, Chongbo, additional, Lu, Jiahong, additional, Zhu, Wenhua, additional, and Zhou, Yan, additional

Published
2020
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18. The Schistosoma japonicum genome reveals features of host-parasite interplay

Author

Zhou, Yan, Zheng, Huajun, Chen, Yangyi, Zhang, Lei, Wang, Kai, Guo, Jing, Huang, Zhen, Zhang, Bo, Huang, Wei, Jin, Ke, Dou, Tonghai, Hasegawa, Masami, Wang, Li, Zhang, Yuan, Zhou, Jie, Tao, Lin, Cao, Zhiwei, Li, Yixue, Vinar, Tomas, Brejova, Brona, Brown, Dan, Li, Ming, Miller, David J., Blair, David, Zhong, Yang, Chen, Zhu, Liu, Feng, Hu, Wei, Wang, Zhi-Qin, Zhang, Qin-Hua, Song, Huai-Dong, Chen, Saijuan, Xu, Xuenian, Xu, Bin, Ju, Chuan, Huang, Yucheng, Brindley, Paul J., McManus, Donald P., Feng, Zheng, Han, Ze-Guang, Lu, Gang, Ren, Shuangxi, Wang, Yuezhu, Gu, Wenyi, Kang, Hui, Chen, Jie, Chen, Xiaoyun, Chen, Shuting, Wang, Lijun, Yan, Jie, Wang, Biyun, Lv, Xinyan, Jin, Lei, Wang, Bofei, Pu, Shiyin, Zhang, Xianglin, Zhang, Wei, Hu, Qiuping, Zhu, Genfeng, Wang, Jun, Yu, Jun, Wang, Jian, Yang, Huanming, Ning, Zemin, Beriman, Matthew, Wei, Chia-Lin, Ruan, Yijun, Zhao, Guoping, and Wang, Shengyue

Published
2009
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21. 5' UTR CGG repeat expansion in GIPC1 is associated with oculopharyngodistal myopathy.

Author

Xi, Jianying, Wang, Xilu, Yue, Dongyue, Dou, Tonghai, Wu, Qunfeng, Lu, Jun, Liu, Yiqi, Yu, Wenbo, Qiao, Kai, Lin, Jie, Luo, Sushan, Li, Jing, Du, Ailian, Dong, Jihong, Chen, Yan, Luo, Lijun, Yang, Jie, Niu, Zhenmin, Liang, Zonghui, and Zhao, Chongbo

Subjects
POLYMERASE chain reaction, MUSCLE diseases, TRINUCLEOTIDE repeats, BLEPHAROPTOSIS, SPINOCEREBELLAR ataxia, DEGENERATION (Pathology), MUSCULAR dystrophy, DNA, GENETIC mutation, SKELETAL muscle, RNA, GENETIC polymorphisms, GENETIC techniques, CARRIER proteins, GENEALOGY
Abstract

Oculopharyngodistal myopathy is a late-onset degenerative muscle disorder characterized by ptosis and weakness of the facial, pharyngeal, and distal limb muscles. A recent report suggested a non-coding trinucleotide repeat expansion in LRP12 to be associated with the disease. Here we report a genetic study in a Chinese cohort of 41 patients with the clinical diagnosis of oculopharyngodistal myopathy (21 cases from seven families and 20 sporadic cases). In a large family with 12 affected individuals, combined haplotype and linkage analysis revealed a maximum two-point logarithm of the odds (LOD) score of 3.3 in chromosomal region chr19p13.11-p13.2 and narrowed the candidate region to an interval of 4.5 Mb. Using a comprehensive strategy combining whole-exome sequencing, long-read sequencing, repeat-primed polymerase chain reaction and GC-rich polymerase chain reaction, we identified an abnormal CGG repeat expansion in the 5' UTR of the GIPC1 gene that co-segregated with disease. Overall, the repeat expansion in GIPC1 was identified in 51.9% independent pedigrees (4/7 families and 10/20 sporadic cases), while the repeat expansion in LRP12 was only identified in one sporadic case (3.7%) in our cohort. The number of CGG repeats was <30 in controls but >60 in affected individuals. There was a slight correlation between repeat size and the age at onset. Both repeat expansion and retraction were observed during transmission but somatic instability was not evident. These results further support that non-coding CGG repeat expansion plays an essential role in the pathogenesis of oculopharyngodistal myopathy. [ABSTRACT FROM AUTHOR]

Published
2021
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29. CTLA4 A49G Polymorphism Shows Significant Association With Glioma Risk in a Chinese Population

Author

Wu, Qihan, primary, Zhan, Xiaoying, additional, Dou, Tonghai, additional, Chen, Hongyan, additional, Fan, Weiwei, additional, Zhou, Keke, additional, Zhang, Haishi, additional, Zheng, Hongxia, additional, Cai, Yanyan, additional, Zhao, Yao, additional, Huang, Fengping, additional, Zhou, Liangfu, additional, Mao, Ying, additional, and Lu, Daru, additional

Published
2010
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39. Cloning and characterization of a novel splice variant of human U2AF1L3 gene

Author

Chen, Fei, Ji, Chaoneng, Dou, Tonghai, Zheng, Nan, Qiu, Rui, Peng, Jing, Fang, Weiqun, Feng, Congjing, Xie, Yi, and Mao, Yumin

Abstract

Pre-mRNA splicing allows individual genes to produce multiple protein isoforms with diverse functions. Recognition of functional splice sites in pre-mRNAs is very important in this splicing process and requires some protein auxiliary factors such as U2 small nuclear ribonucleoprotein auxiliary factor small subunit (U2AF35, encoded by U2AF1). By its RNA binding domains, U2AF35 interacts with U2AF65 to bind 3' splice site of pre-mRNA and initiates splicing. Another protein, which is named as U2AF1-like3 (U2AF1L3), shows high similarity with U2AF35 and may have related function in pre-mRNA splicing. Here, we report a splice variant of U2AF1L3, which is 767 bp in length and has an open reading frame (ORF) coding a predicted 181 amino acids protein. Reverse transcription-PCR (RT-PCR) shows that this isoform has different expression pattern with U2AF1L3 and is highly expressed in heart, brain and lung.

Published
2006
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40. Cloning and Characterization of a novel splice variant of human Rab18 gene (RAB18)

Author

Dou, Tonghai, Ji, Chaoneng, Gu, Shaohua, Chen, Fei, Xu, Jiaxi, Ye, Xin, Ying, Kang, Xie, Yi, and Mao, Yumin

Abstract

Rab GTPase proteins are a kind of small GTP-binding proteins, which functions mainly focus on regulating interacellular trafficking pathways during vesicular transport. To date, 60 distinct human RAB proteins have been identified. RAB18 gene is discovered from endothelial cells. Its function is considered as endosomes and plasma membrane recycling. Research indicates RAB18 may relate to inflammation and some kinds of tumor. Here we report a splice variant of RAB18, which is 2571 bp in length and has an open reading frame coding a predicted 235 amino-acids protein. RT-PCR shows that the cDNA has different expression pattern with RAB18 and is highly expressed in testis.

Published
2005
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42. 5' UTR CGG repeat expansion in GIPC1 is associated with oculopharyngodistal myopathy.

Author

Xi J, Wang X, Yue D, Dou T, Wu Q, Lu J, Liu Y, Yu W, Qiao K, Lin J, Luo S, Li J, Du A, Dong J, Chen Y, Luo L, Yang J, Niu Z, Liang Z, Zhao C, Lu J, Zhu W, and Zhou Y

Subjects
5' Untranslated Regions, Adult, Aged, Aged, 80 and over, Asian People genetics, Female, Humans, Male, Middle Aged, Muscle, Skeletal pathology, Muscular Dystrophies pathology, Mutation, Pedigree, Polymorphism, Single Nucleotide, Exome Sequencing, Adaptor Proteins, Signal Transducing genetics, Muscular Dystrophies genetics, Trinucleotide Repeat Expansion
Abstract

Oculopharyngodistal myopathy is a late-onset degenerative muscle disorder characterized by ptosis and weakness of the facial, pharyngeal, and distal limb muscles. A recent report suggested a non-coding trinucleotide repeat expansion in LRP12 to be associated with the disease. Here we report a genetic study in a Chinese cohort of 41 patients with the clinical diagnosis of oculopharyngodistal myopathy (21 cases from seven families and 20 sporadic cases). In a large family with 12 affected individuals, combined haplotype and linkage analysis revealed a maximum two-point logarithm of the odds (LOD) score of 3.3 in chromosomal region chr19p13.11-p13.2 and narrowed the candidate region to an interval of 4.5 Mb. Using a comprehensive strategy combining whole-exome sequencing, long-read sequencing, repeat-primed polymerase chain reaction and GC-rich polymerase chain reaction, we identified an abnormal CGG repeat expansion in the 5' UTR of the GIPC1 gene that co-segregated with disease. Overall, the repeat expansion in GIPC1 was identified in 51.9% independent pedigrees (4/7 families and 10/20 sporadic cases), while the repeat expansion in LRP12 was only identified in one sporadic case (3.7%) in our cohort. The number of CGG repeats was <30 in controls but >60 in affected individuals. There was a slight correlation between repeat size and the age at onset. Both repeat expansion and retraction were observed during transmission but somatic instability was not evident. These results further support that non-coding CGG repeat expansion plays an essential role in the pathogenesis of oculopharyngodistal myopathy., (© The Author(s) (2020). Published by Oxford University Press on behalf of the Guarantors of Brain. All rights reserved. For permissions, please email: journals.permissions@oup.com.)

Published
2021
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43. Evolution of peroxisome proliferator-activated receptor gamma alternative splicing.

Author

Dou T, Xu J, Gao Y, Gu J, Ji C, Xie Y, and Zhou Y

Subjects
Animals, Exons, Humans, Polymorphism, Single Nucleotide, Alternative Splicing, Evolution, Molecular, PPAR gamma genetics
Abstract

Peroxisome proliferator-activated receptor gamma (PPAR gamma) plays an important role in the control of energy balance and lipid and glucose homeostasis. Different transcript variants were investigated not only in human but also in other vertebrates. To look into the evolutionary changes of these variants, we analyzed the genomic sequences of PPAR gamma genes from several vertebrate species, as well as their mRNA and EST data. Several potential alternative splicing exons at the 5'-end of the PPAR gamma gene were identified. The 5'-end of the PPAR gamma gene is discovered to be evolutionarily active and recruits new exons via different strategies. Moreover, it is shown that the only coding alternative exon (exon B) processes much higher Ka/Ks compared with its constitutive counterparts. In addition, its Ka/Ks is greater than 1 in the rat, mouse, and rabbit, indicating adaptive evolution and possible energy storage related gain-of-function for the exon.

Published
2010
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