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A simple and sensitive extractive spectrophotometric method have been developed and validated for determination of amantadine hydrochloride (AM), memantine hydrochloride (MM) and rimantadine hydrochloride (RM) in pure and pharmaceutical formulations. The method is based on the reaction of these active compounds with bromophenol blue (BB) in acetate buffer (0.1 M) pH 3.5 to form an orange-colored products which have absorption maxima at 408 nm. The procedure of complexation was optimized with regard to such factors as concentrations of BB, pH of medium, a kind of extracting solvents and a number of extractions. Under the optimum conditions, linear relationships A408 = f(c) with good correlation coefficients (≥0.996) and low limit of detection were obtained in the ranges of 50.0-220.0 µg·mL−1, 20.0-150.0 µg·mL−1 or 10.0-110.0 µg·mL−1 for AM, MM and RM, respectively, for the spectrophotometric methods. The proposed method could be applied to the determination of AM, MM and RM in dosage forms. The recovery was 95.3-101.9%. The method was linear, precise and accurate. [ABSTRACT FROM AUTHOR]