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Watermelon (Citrullus lanatus) is a fruit widely consumed by the Brazilian population; however, its rind is usually discarded, despite its nutritional value. This work aimed to develop a watermelon rind flour (WRF) and a fiber-rich bread. The WRF was submitted to microbiological analysis, proximate composition, antioxidant activity, and a profile of phenolic compounds. Six types of bread were developed: three using WRF (20%, 30%, and 40% of wheat flour replacement) and three control samples (only wheat flour), which were submitted to the same analysis, in addition to colorimetric properties, instrumental texture profile, and sensory evaluation (check-all-that-apply, purchase intent, and acceptance test). A high fiber content was observed in WRF (27.15%). The total concentration of phenolic compounds was 2.38 ± 0.20 mg of gallic acid equivalent per g and benzoic acid was the main compound found (73.50 mg/100 g WRF). The results of the antioxidant capacity through the DPPH method indicated a 30% reduction. The WRF 40% bread had 3.06 g of insoluble fiber, characterizing itself as a source of fiber. In the instrumental texture analysis, it was observed that bread hardness increased significantly as the WRF content increased. The formulation of the bread containing WRF is an alternative for the use of waste, favoring the development of a fiber-source product, with a functional food claim., (© 2023 Institute of Food Technologists.)

Gastrointestinal nematode infections have caused expressive losses in sheep production worldwide. The improvement of host genetic resistance to worms has been used as a strategy to mitigate this problem. In this sense, the inclusion of genomic information has shown potential to increase the accuracy of prediction of breeding values and speed up selection. In this study, we aimed to compare estimates of genetic parameters and breeding values for traits that indicate the resistance to gastrointestinal nematode infection in Santa Inês sheep using the pedigree-based BLUP or including genomic information. There were 1478 animals in the pedigree, of which 271 were genotyped using the OvineSNP50 BeadChip (Illumina, Inc.). The host resistance was assessed using the following traits: fecal nematode egg counts (FEC); FAMACHA score (FAMACHA); and resistance to gastrointestinal nematode infection (RGNI) as a combination of FEC, FAMACHA, body condition score, and hematocrit. The genetic parameters and breeding values were estimated using single- and multi-trait analyses. For RGNI, the heritability estimates ranged from 0.25 using the single-trait genomic model (S-H) to 0.54 using the traditional multi-trait model (M-A). The heritability estimates for FEC ranged from 0.06 to 0.36, using the single-trait pedigree-based model (S-A) and the multi-trait genomic model (M-H), respectively. For FAMACHA, the heritability estimates ranged from 0.46 (M-H) to 0.54 (M-A). Estimates of genetic correlation ranged from 0.22 to 0.69. The inclusion of genomic information provided gain in accuracy for all traits. All estimates of predictive ability obtained using genomic data in a multi-trait setting were higher than those obtained using single-trait models. The estimates of predictive ability ranged from 0.03 (S-A) to 0.46 (M-H). The heritability estimates obtained using genomic information showed that all traits evaluated are suitable for genomic selection. Despite the low accuracies obtained, the use of the genomic model provided more accurate estimates of breeding values in comparison to the pedigree-based model.

Objective: To investigate the recovery of male water polo athletes applying full body photobiomodulation therapy (PBMT) regarding inflammation and muscle damage markers, testosterone and cortisol hormonal responses, heart rate variability (HRV), maximal voluntary contraction, and squat jump (SJ) after official water polo matches. Background: PBMT has been applied locally on specific muscle groups to induce faster recovery and improve the performance of athletes and nonathletes. However, many sports modalities require movement of the whole body, and a full body PBMT could be more adequate to irradiate large muscle areas homogeneously and faster. Methods: In a randomized, parallel, and double-blinded design, 13 athletes (whole team) aged 18 years attended the study and were allocated into two groups: PBMT (dose of 6.9 J/cm 2 , irradiance of 46.17 mW/cm 2 , 5 min irradiation) and placebo treatment. The study was conducted during the 2019 Brazilian under 20 water polo championship. All athletes were assessed by blood samples and neuromuscular evaluation. Immediately after each match, all athletes received PBMT (effective or placebo). Results: No significant interactions (raw values and percentage related to baseline) were observed for testosterone and cortisol, tumor necrosis factor-alpha, interleukin-6, creatine kinase concentration, maximal isometric voluntary contraction, SJ test, and HRV. Only an isolated interaction (decrease) was found for lactate dehydrogenase (LDH) response after the first match ( p  = 0.004, post-hoc p  = 0.038). Conclusions: The parameters of the full body PBMT of this study did not induce faster recovery of inflammatory, muscle damage (excepting LDH), testosterone, cortisol, HRV, and neuromuscular responses during repeated days of water polo matches.

Background: Photobiomodulation therapy (PBMT) by lasers or light-emitting diodes (LEDs) has been used in the last two decades to increase muscle performance in humans. The main findings of PBMT on muscle performance are increment in torque and number of muscle contractions, and fatigue resistance in high-intensity exercises. Although there are suggested light energies to be followed, the literature has also pointed out to a possible time-response of PBMT to increase exercise performance and recovery in humans. Objective: To investigate the possible time-response of PBMT by LEDs to increase muscle performance in young men regarding peak torque (PT), rate of torque development (RTD), fatigue resistance, and subjective perception of effort in maximal voluntary isometric contractions (MVIC) of elbow flexion. Methods: This randomized, double-blind, placebo-controlled, crossover trial with two arms enrolled 34 healthy and physically active young men, but 30 (21.10 ± 2.25 years old) completed all procedures. All volunteers were allocated into two equal arms (groups): PBMT (60 J; 1152 mW; 52 sec; and 166.75 cm 2 ) applied on biceps brachii by a flexible array of LEDs, and placebo treatment. Each arm ( n  = 15) investigated the time-response (5 min, 1 h, 3 h, and 6 h) of PBMT in a randomized, double-blind, and crossover manner on the PT, RTD, fatigue resistance, and subjective perception of effort. Results: There were no significant results ( p  > 0.05) for all comparisons within and between groups regarding PT, RTD, fatigue resistance, and subjective perception of effort in MVIC of the elbow flexion in all time-response tested (5 min, 1 h, 3 h, and 6 h) before or after fatigue test. Only RTD did not decay significantly after fatigue test at all time-responses for PBMT group. Conclusions: PBMT was not effective to increase muscle performance and decrease fatigue to demonstrate the possible time-response in humans.

To investigate the effects of photobiomodulation applied to respiratory muscles on lung function, thoracoabdominal mobility, respiratory muscle strength, and functional capacity in COPD patients. This is a randomized double-blind crossover clinical trial. Twelve male COPD patients participated in the study. Participants were randomly allocated to receive two photobiomodulation sessions, 1 week apart: (1) an effective photobiomodulation session applied at the main respiratory muscles by means of a cluster with 69 light-emitting diodes (LEDs), containing 35 red (630 ± 10 nm; 10 mW; 0.2 cm 2 ) and 34 near-infrared (830 ± 20 nm; 10 mW; 0.2 cm 2 ) LEDs and (2) a sham photobiomodulation session, following the same procedures without emitting light. The primary outcomes were pulmonary function (spirometric indexes); thoracoabdominal mobility (cirtometry); respiratory muscle strength (maximal respiratory pressures), assessed at three moments: (1) baseline, (2) 1 h after intervention, and (3) 24 h after intervention; and the functional capacity, assessed by the 6-min walk test (6MWT) at baseline and 24 h after intervention. No significant interactions were found for spirometric variables, maximal respiratory pressures, and cirtometry. However, there was a Time × Condition interaction (F = 18.63; p = 0.001; η 2 p  = 0.62) in the walked distance on the 6MWT, with a significant increase after photobiomodulation intervention (p < 0.01) compared with the baseline. Photobiomodulation applied to respiratory muscles was effective in improving acute functional capacity in COPD patients. To the best of our knowledge, this is the first study assessing the effects of photobiomodulation applied to respiratory muscles in patients with COPD.

Interleukin 4 (IL-4) is a pleiotropic cytokine involved in many functions during the development as well as in adult life. Previous work from our group demonstrated, in vitro, that this interleukin is able to prevent rat retinal ganglion cells death after axotomy. The aim of the present study was to investigate the signaling pathways involved in this trophic effect, particularly the cAMP pathway and also to demonstrate the expression of IL-4 in retinas at different stages of post natal development. Our results show that the trophic effect of IL-4 on rat retinal ganglion cells is dependent on the activation of Janus Kinase 3, Protein Kinase A, c-Jun N-terminal Kinase and Tropomyosin related Kinase receptors, on the increase in intracellular calcium levels, on polypeptide release and on the endogenous Brain Derived Neurotrophic Factor (BDNF). We also observed that treatment with IL-4 enhances c-AMP response element binding and Mitogen Activated Protein Kinase phosphorylation and increases the expression of BDNF. Concerning the IL-4 expression our data show an increase in IL-4 levels during post natal development. Taken together our results demonstrate that the trophic effect of IL-4 on retinal ganglion cells of newborn rats is mediated by cAMP pathway and BDNF release.