1. A novel loop-mediated isothermal amplification method for identification of four body fluids with smartphone detection
- Author
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An-Chi Tsuei, Kimberly Jackson, James P. Landers, David A. Dent, Dorris M. Haverstick, Tiffany Layne, and Jingyi Li
- Subjects
Forensic Genetics ,Male ,0301 basic medicine ,beta-Defensins ,Smart phone ,Computer science ,Loop-mediated isothermal amplification ,Histatins ,Seminal Vesicle Secretory Proteins ,Sensitivity and Specificity ,Pathology and Forensic Medicine ,03 medical and health sciences ,0302 clinical medicine ,Blind study ,Semen ,Image Processing, Computer-Assisted ,Genetics ,Humans ,Contextual information ,Sample preparation ,RNA, Messenger ,030216 legal & forensic medicine ,Saliva ,Microwell Plate ,business.industry ,Globins ,Blood ,030104 developmental biology ,Molecular Diagnostic Techniques ,Vaginal fluid ,Cervix Mucus ,Female ,Smartphone ,business ,Nucleic Acid Amplification Techniques ,Biomarkers ,Computer hardware - Abstract
Messenger RNA profiling for body fluid identification (bfID) is a useful approach to collect contextual information associated with a crime. Current methods require costly fluorescent probes, lengthy amplification protocols and/or time-consuming sample preparation. To simplify this process, we developed a bfID method that has the potential to be rapid in analysis time, inexpensive and fluorescence-free, combining a universal operating procedure with a high-throughout (microwell plate) platform for simultaneous detection of mRNA markers from whole blood, semen, saliva, and vaginal fluid. Full bfID sample preparation and analysis of 23 samples was completed in under 3 h using smart phone optical detection and analysis and show efficacy of the method in a validated blind study. The results provide an efficient, sensitive and specific approach to supplement the current biochemical tests in a forensic laboratory.
- Published
- 2020
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