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5 results on '"Dorner, Brigitte Gertrud"'

1. A large travel-associated outbreak of iatrogenic botulism in four European countries following intragastric botulinum neurotoxin injections for weight reduction, Türkiye, February to March 2023

Author

Dorner, Martin Bernhard, primary, Wilking, Hendrik, additional, Skiba, Martin, additional, Wilk, Laura, additional, Steinberg, Maximilian, additional, Worbs, Sylvia, additional, Çeken, Sabahat, additional, Kaygusuz, Sedat, additional, Simon, Stéphanie, additional, Becher, François, additional, Mikolajewska, Agata, additional, Kornschober, Christian, additional, Bütler, Timo, additional, Jourdan-Da-Silva, Nathalie, additional, an der Heiden, Maria, additional, Schaade, Lars, additional, Stark, Klaus, additional, Dorner, Brigitte Gertrud, additional, and Frank, Christina, additional

Published
2023
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2. First cross-border outbreak of foodborne botulism in the European Union associated with the consumption of commercial dried roach (Rutilus rutilus)

Author

Hendrickx, David, primary, Varela Martínez, Carmen, additional, Contzen, Matthias, additional, Wagner-Wiening, Christiane, additional, Janke, Karl-Heinz, additional, Hernando Jiménez, Pablo, additional, Massing, Susanne, additional, Pichler, Jeanette, additional, Tichaczek-Dischinger, Petra, additional, Burckhardt, Florian, additional, Stark, Klaus, additional, Katz, Katharina, additional, Jurke, Annette, additional, Thole, Sebastian, additional, Carbó, Rosa, additional, del Pobil Ferré, Mariam Pascual, additional, Nieto, Milagros, additional, Zamora, María Jesús, additional, Sisó, Ana, additional, Pallares García, Pilar, additional, Valdezate, Sylvia, additional, Schaade, Lars, additional, Worbs, Sylvia, additional, Dorner, Brigitte Gertrud, additional, Frank, Christina, additional, and Dorner, Martin Bernhard, additional

Published
2023
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4. A lipid-binding loop of botulinum neurotoxin serotypes B, DC and G is an essential feature to confer their exquisite potency

Author

Stern, Daniel, primary, Weisemann, Jasmin, additional, Le Blanc, Alexander, additional, von Berg, Laura, additional, Mahrhold, Stefan, additional, Piesker, Janett, additional, Laue, Michael, additional, Luppa, Peter B., additional, Dorner, Martin Bernhard, additional, Dorner, Brigitte Gertrud, additional, and Rummel, Andreas, additional

Published
2018
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5. Only the complex N559-glycan in the synaptic vesicle glycoprotein 2C mediates high affinity binding to botulinum neurotoxin serotype A1.

Author

Mahrhold, Stefan, Bergström, Tomas, Stern, Daniel, Dorner, Brigitte Gertrud, Åstot, Crister, and Rummel, Andreas

Subjects
SYNAPTIC vesicles, GLYCOPROTEINS, BOTULINUM A toxins, MOTOR neurons, ENDOCYTOSIS, PROTEIN-protein interactions
Abstract

The extraordinary potency of botulinum neurotoxins (BoNTs) is mediated by their high neurospecificity, targeting peripheral cholinergic motoneurons leading to flaccid paralysis and successive respiratory failure. Complex polysialo gangliosides accumulate BoNTs on the plasma membrane and facilitate subsequent binding to synaptic vesicle membrane proteins which results in toxin endocytosis. The luminal domain 4 (LD4) of the three synaptic vesicle glycoprotein 2 (SV2) isoforms A-C mediates uptake of the clinically most relevant serotype BoNT/A1. SV2C-LD4 exhibits the strongest protein-protein interaction and comprises five putative N-glycosylation sites (PNG sites). Here, we expressed human SV2C-LD4 fused to human IgG-Fc in prokaryotic and eukaryotic expression systems to analyse the effect of N-glycosylation of SV2C on the interaction with BoNT/A1. Mass spectrometric analysis of gSV2CLDFc demonstrates glycosylation of N534, N559 and N565, the latter two residing at the BoNT/A interface. Mutational analysis demonstrates that only the N559-glycan, but not N565-glycan increases affinity of BoNT/A for human gSV2C-LD4. The N559- glycan was characterised as a complex core-fucosylated type with a heterogeneity ranging up to tetra-antennary structure with bisecting N-acetylglucosamine which can establish extensive interactions with BoNT/A. The mutant gSV2CLD-Fc N559A displayed a 50-fold increased dissociation rate kd resulting in an overall 12-fold decreased binding affinity in surface plasmon resonance (SPR) experiments. The delayed dissociation might provide BoNT/Amore time for endocytosis into synaptic vesicles. In conclusion, we show the importance of the complex N559- glycan of SV2C-LD4, adding a third anchor point beside a ganglioside and the SV2C-LD4 peptide, for BoNT/A neuronal cell surface binding and uptake. [ABSTRACT FROM AUTHOR]

Published
2016
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