12 results on '"Dorgans K"'
Search Results
2. Inhibition promotes long-term potentiation at cerebellar excitatory synapses
- Author
-
Binda, F., primary, Dorgans, K., additional, Reibel, S., additional, Sakimura, K., additional, Kano, M., additional, Poulain, B., additional, and Isope, P., additional
- Published
- 2016
- Full Text
- View/download PDF
3. Designing AAV Vectors for Monitoring the Subtle Calcium Fluctuations of Inferior Olive Network in vivo .
- Author
-
Dorgans K, Guo D, Kurima K, Wickens J, and Uusisaari MY
- Abstract
Adeno-associated viral (AAV) vectors, used as vehicles for gene transfer into the brain, are a versatile and powerful tool of modern neuroscience that allow identifying specific neuronal populations, monitoring and modulating their activity. For consistent and reproducible results, the AAV vectors must be engineered so that they reliably and accurately target cell populations. Furthermore, transgene expression must be adjusted to sufficient and safe levels compatible with the physiology of studied cells. We undertook the effort to identify and validate an AAV vector that could be utilized for researching the inferior olivary (IO) nucleus, a structure gating critical timing-related signals to the cerebellum. By means of systematic construct generation and quantitative expression profiling, we succeeded in creating a viral tool for specific and strong transfection of the IO neurons without adverse effects on their physiology. The potential of these tools is demonstrated by expressing the calcium sensor GCaMP6s in adult mouse IO neurons. We could monitor subtle calcium fluctuations underlying two signatures of intrinsic IO activity: the subthreshold oscillations (STOs) and the variable-duration action potential waveforms both in-vitro and in-vivo . Further, we show that the expression levels of GCaMP6s allowing such recordings are compatible with the delicate calcium-based dynamics of IO neurons, inviting future work into the network dynamics of the olivo-cerebellar system in behaving animals., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Dorgans, Guo, Kurima, Wickens and Uusisaari.)
- Published
- 2022
- Full Text
- View/download PDF
4. Cerebellar connectivity maps embody individual adaptive behavior in mice.
- Author
-
Spaeth L, Bahuguna J, Gagneux T, Dorgans K, Sugihara I, Poulain B, Battaglia D, and Isope P
- Subjects
- Animals, Animals, Newborn, Male, Mice, Motor Activity physiology, Purkinje Cells physiology, Synapses physiology, Adaptation, Psychological physiology, Behavior, Animal physiology, Cerebellum physiology, Nerve Net physiology
- Abstract
The cerebellar cortex encodes sensorimotor adaptation during skilled locomotor behaviors, however the precise relationship between synaptic connectivity and behavior is unclear. We studied synaptic connectivity between granule cells (GCs) and Purkinje cells (PCs) in murine acute cerebellar slices using photostimulation of caged glutamate combined with patch-clamp in developing or after mice adapted to different locomotor contexts. By translating individual maps into graph network entities, we found that synaptic maps in juvenile animals undergo critical period characterized by dissolution of their structure followed by the re-establishment of a patchy functional organization in adults. Although, in adapted mice, subdivisions in anatomical microzones do not fully account for the observed spatial map organization in relation to behavior, we can discriminate locomotor contexts with high accuracy. We also demonstrate that the variability observed in connectivity maps directly accounts for motor behavior traits at the individual level. Our findings suggest that, beyond general motor contexts, GC-PC networks also encode internal models underlying individual-specific motor adaptation., (© 2022. The Author(s).)
- Published
- 2022
- Full Text
- View/download PDF
5. Imaging Subthreshold Voltage Oscillation With Cellular Resolution in the Inferior Olive in vitro .
- Author
-
Dorgans K, Kuhn B, and Uusisaari MY
- Abstract
Voltage imaging with cellular resolution in mammalian brain slices is still a challenging task. Here, we describe and validate a method for delivery of the voltage-sensitive dye ANNINE-6plus (A6+) into tissue for voltage imaging that results in higher signal-to-noise ratio (SNR) than conventional bath application methods. The not fully dissolved dye was injected into the inferior olive (IO) 0, 1, or 7 days prior to acute slice preparation using stereotactic surgery. We find that the voltage imaging improves after an extended incubation period in vivo in terms of labeled volume, homogeneous neuropil labeling with saliently labeled somata, and SNR. Preparing acute slices 7 days after the dye injection, the SNR is high enough to allow single-trial recording of IO subthreshold oscillations using wide-field (network-level) as well as high-magnification (single-cell level) voltage imaging with a CMOS camera. This method is easily adaptable to other brain regions where genetically-encoded voltage sensors are prohibitively difficult to use and where an ultrafast, pure electrochromic sensor, like A6+, is required. Due to the long-lasting staining demonstrated here, the method can be combined, for example, with deep-brain imaging using implantable GRIN lenses., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2020 Dorgans, Kuhn and Uusisaari.)
- Published
- 2020
- Full Text
- View/download PDF
6. Voltage Imaging of Cortical Oscillations in Layer 1 with Two-Photon Microscopy.
- Author
-
Dalphin N, Dorgans K, Khaskin E, and Kuhn B
- Subjects
- Animals, Membrane Potentials, Mice, Somatosensory Cortex, Wakefulness, Microscopy, Neurons
- Abstract
Membrane voltage oscillations in layer 1 (L1) of primary sensory cortices might be important indicators of cortical gain control, attentional focusing, and signal integration. However, electric field recordings are hampered by the low seal resistance of electrodes close to the brain surface. To study L1 membrane voltage oscillations, we synthesized a new voltage-sensitive dye, di1-ANNINE (anellated hemicyanine)-6plus, that can diffuse into tissue. We applied it with a new surgery, leaving the dura intact but allowing injection of large quantities of staining solution, and imaged cortical membrane potential oscillations with two-photon microscopy depth-resolved (25-100 μm below dura) in anesthetized and awake mice. We found delta (0.5-4 Hz), theta (4-10 Hz), low beta (10-20 Hz), and low gamma (30-40 Hz) oscillations. All oscillations were stronger in awake animals. While the power of delta, theta, and low beta oscillations increased with depth, the power of low gamma was more constant throughout L1. These findings identify L1 as an important coordination hub for the dynamic binding process of neurons mediated by oscillations., (Copyright © 2020 Dalphin et al.)
- Published
- 2020
- Full Text
- View/download PDF
7. Gradients in the mammalian cerebellar cortex enable Fourier-like transformation and improve storing capacity.
- Author
-
Straub I, Witter L, Eshra A, Hoidis M, Byczkowicz N, Maas S, Delvendahl I, Dorgans K, Savier E, Bechmann I, Krueger M, Isope P, and Hallermann S
- Subjects
- Animals, Biophysical Phenomena physiology, Fourier Analysis, Mice, Models, Neurological, Nerve Fibers metabolism, Nerve Fibers physiology, Purkinje Cells cytology, Purkinje Cells metabolism, Purkinje Cells physiology, Synaptic Potentials physiology, White Matter cytology, White Matter metabolism, White Matter physiology, Cerebellar Cortex cytology, Cerebellar Cortex metabolism, Cerebellar Cortex physiology, Neurons cytology, Neurons metabolism, Neurons physiology
- Abstract
Cerebellar granule cells (GCs) make up the majority of all neurons in the vertebrate brain, but heterogeneities among GCs and potential functional consequences are poorly understood. Here, we identified unexpected gradients in the biophysical properties of GCs in mice. GCs closer to the white matter (inner-zone GCs) had higher firing thresholds and could sustain firing with larger current inputs than GCs closer to the Purkinje cell layer (outer-zone GCs). Dynamic Clamp experiments showed that inner- and outer-zone GCs preferentially respond to high- and low-frequency mossy fiber inputs, respectively, enabling dispersion of the mossy fiber input into its frequency components as performed by a Fourier transformation. Furthermore, inner-zone GCs have faster axonal conduction velocity and elicit faster synaptic potentials in Purkinje cells. Neuronal network modeling revealed that these gradients improve spike-timing precision of Purkinje cells and decrease the number of GCs required to learn spike-sequences. Thus, our study uncovers biophysical gradients in the cerebellar cortex enabling a Fourier-like transformation of mossy fiber inputs., Competing Interests: IS, LW, AE, MH, NB, SM, ID, KD, ES, IB, MK, PI, SH No competing interests declared, (© 2020, Straub et al.)
- Published
- 2020
- Full Text
- View/download PDF
8. Differential Coding Strategies in Glutamatergic and GABAergic Neurons in the Medial Cerebellar Nucleus.
- Author
-
Özcan OO, Wang X, Binda F, Dorgans K, De Zeeuw CI, Gao Z, Aertsen A, Kumar A, and Isope P
- Subjects
- Action Potentials, Afferent Pathways physiology, Anesthesia, Animals, Cerebellar Nuclei cytology, Channelrhodopsins physiology, Genes, Reporter, Glutamate Decarboxylase genetics, Interneurons physiology, Male, Mice, Mice, Inbred C57BL, Motor Skills, Neurons physiology, Optogenetics, Time Factors, Vesicular Glutamate Transport Protein 2 genetics, Wakefulness, Cerebellar Nuclei physiology, GABAergic Neurons physiology, Glutamic Acid physiology, Purkinje Cells physiology
- Abstract
The cerebellum drives motor coordination and sequencing of actions at the millisecond timescale through adaptive control of cerebellar nuclear output. Cerebellar nuclei integrate high-frequency information from both the cerebellar cortex and the two main excitatory inputs of the cerebellum: the mossy fibers and the climbing fiber collaterals. However, how nuclear cells process rate and timing of inputs carried by these inputs is still debated. Here, we investigate the influence of the cerebellar cortical output, the Purkinje cells, on identified cerebellar nuclei neurons in vivo in male mice. Using transgenic mice expressing Channelrhodopsin2 specifically in Purkinje cells and tetrode recordings in the medial nucleus, we identified two main groups of neurons based on the waveform of their action potentials. These two groups of neurons coincide with glutamatergic and GABAergic neurons identified by optotagging after Chrimson expression in VGLUT2-cre and GAD-cre mice, respectively. The glutamatergic-like neurons fire at high rate and respond to both rate and timing of Purkinje cell population inputs, whereas GABAergic-like neurons only respond to the mean population firing rate of Purkinje cells at high frequencies. Moreover, synchronous activation of Purkinje cells can entrain the glutamatergic-like, but not the GABAergic-like, cells over a wide range of frequencies. Our results suggest that the downstream effect of synchronous and rhythmic Purkinje cell discharges depends on the type of cerebellar nuclei neurons targeted. SIGNIFICANCE STATEMENT Motor coordination and skilled movements are driven by the permanent discharge of neurons from the cerebellar nuclei that communicate cerebellar computation to other brain areas. Here, we set out to study how specific subtypes of cerebellar nuclear neurons of the medial nucleus are controlled by Purkinje cells, the sole output of the cerebellar cortex. We could isolate different subtypes of nuclear cell that differentially encode Purkinje cell inhibition. Purkinje cell stimulation entrains glutamatergic projection cells at their firing frequency, whereas GABAergic neurons are only inhibited. These differential coding strategies may favor temporal precision of cerebellar excitatory outputs associated with specific features of movement control while setting the global level of cerebellar activity through inhibition via rate coding mechanisms., (Copyright © 2020 the authors.)
- Published
- 2020
- Full Text
- View/download PDF
9. Short-term plasticity at cerebellar granule cell to molecular layer interneuron synapses expands information processing.
- Author
-
Dorgans K, Demais V, Bailly Y, Poulain B, Isope P, and Doussau F
- Subjects
- Animals, Mice, Synapsins metabolism, Cerebellum cytology, Cerebellum physiology, Nerve Net physiology, Neuronal Plasticity physiology, Neurons physiology
- Abstract
Information processing by cerebellar molecular layer interneurons (MLIs) plays a crucial role in motor behavior. MLI recruitment is tightly controlled by the profile of short-term plasticity (STP) at granule cell (GC)-MLI synapses. While GCs are the most numerous neurons in the brain, STP diversity at GC-MLI synapses is poorly documented. Here, we studied how single MLIs are recruited by their distinct GC inputs during burst firing. Using slice recordings at individual GC-MLI synapses of mice, we revealed four classes of connections segregated by their STP profile. Each class differentially drives MLI recruitment. We show that GC synaptic diversity is underlain by heterogeneous expression of synapsin II, a key actor of STP and that GC terminals devoid of synapsin II are associated with slow MLI recruitment. Our study reveals that molecular, structural and functional diversity across GC terminals provides a mechanism to expand the coding range of MLIs., Competing Interests: KD, VD, YB, BP, PI, FD No competing interests declared, (© 2019, Dorgans et al.)
- Published
- 2019
- Full Text
- View/download PDF
10. Frequency-dependent mobilization of heterogeneous pools of synaptic vesicles shapes presynaptic plasticity.
- Author
-
Doussau F, Schmidt H, Dorgans K, Valera AM, Poulain B, and Isope P
- Subjects
- Action Potentials, Animals, Mice, Synaptic Transmission, Cerebellum physiology, Neuronal Plasticity, Neurons physiology, Presynaptic Terminals metabolism, Synaptic Vesicles metabolism
- Abstract
The segregation of the readily releasable pool of synaptic vesicles (RRP) in sub-pools that are differentially poised for exocytosis shapes short-term plasticity. However, the frequency-dependent mobilization of these sub-pools is poorly understood. Using slice recordings and modeling of synaptic activity at cerebellar granule cell to Purkinje cell synapses of mice, we describe two sub-pools in the RRP that can be differentially recruited upon ultrafast changes in the stimulation frequency. We show that at low-frequency stimulations, a first sub-pool is gradually silenced, leading to full blockage of synaptic transmission. Conversely, a second pool of synaptic vesicles that cannot be released by a single stimulus is recruited within milliseconds by high-frequency stimulation and support an ultrafast recovery of neurotransmitter release after low-frequency depression. This frequency-dependent mobilization or silencing of sub-pools in the RRP in terminals of granule cells may play a role in the filtering of sensorimotor information in the cerebellum.
- Published
- 2017
- Full Text
- View/download PDF
11. Characterization of the dominant inheritance mechanism of Episodic Ataxia type 2.
- Author
-
Dorgans K, Salvi J, Bertaso F, Bernard L, Lory P, Doussau F, and Mezghrani A
- Subjects
- Animals, Ataxia pathology, Cerebellum pathology, Disease Models, Animal, Gene Knock-In Techniques, Genes, Dominant, Mice, Inbred C57BL, Mice, Transgenic, Motor Activity physiology, Muscle Weakness genetics, Muscle Weakness metabolism, Muscle Weakness pathology, Neurons pathology, Nystagmus, Pathologic pathology, Phenotype, Seizures genetics, Seizures metabolism, Seizures pathology, Synapses metabolism, Tissue Culture Techniques, Ataxia genetics, Ataxia metabolism, Calcium Channels, N-Type genetics, Calcium Channels, N-Type metabolism, Cerebellum metabolism, Neurons metabolism, Nystagmus, Pathologic genetics, Nystagmus, Pathologic metabolism
- Abstract
Episodic Ataxia type 2 (EA2) is an autosomal dominant neuronal disorder linked to mutations in the Ca
v 2.1 subunit of P/Q-type calcium channels. In vitro studies have established that EA2 mutations induce loss of channel activity and that EA2 mutants can exert a dominant negative effect, suppressing normal Cav 2.1 activity through protein misfolding and trafficking defects. To date, the role of this mechanism in the disease pathogenesis is unknown because no animal model exists. To address this issue, we have generated a mouse bearing the R1497X nonsense mutation in Cav 2.1 (Cav 2.1R1497X ). Phenotypic analysis of heterozygous Cav 2.1R1497X mice revealed ataxia associated with muscle weakness and generalized absence epilepsy. Electrophysiological studies of the cerebellar circuits in heterozygous Cav 2.1R1497X mice highlighted severe dysregulations in synaptic transmission of the two major excitatory inputs as well as alteration of the spontaneous activity of Purkinje cells. Moreover, these neuronal dysfunctions were associated with a strong suppression of Cav 2.1 channel expression in the cerebellum of heterozygous Cav 2.1R1497X mice. Finally, the presence of Cav 2.1 in cerebellar lipid raft microdomains was strongly impaired in heterozygous Cav 2.1R1497X mice. Altogether, these results reveal a pathogenic mechanism for EA2 based on a dominant negative activity of mutant channels., (Copyright © 2017. Published by Elsevier Inc.)- Published
- 2017
- Full Text
- View/download PDF
12. Late-Life Environmental Enrichment Induces Acetylation Events and Nuclear Factor κB-Dependent Regulations in the Hippocampus of Aged Rats Showing Improved Plasticity and Learning.
- Author
-
Neidl R, Schneider A, Bousiges O, Majchrzak M, Barbelivien A, de Vasconcelos AP, Dorgans K, Doussau F, Loeffler JP, Cassel JC, and Boutillier AL
- Subjects
- Acetylation, Animals, Brain-Derived Neurotrophic Factor metabolism, Chromatin metabolism, Epigenesis, Genetic, Female, Gene Expression genetics, Maze Learning physiology, Neurogenesis physiology, Rats, Rats, Long-Evans, Spatial Memory physiology, Synapses physiology, Transcription Factor RelA genetics, Transcription Factor RelA metabolism, Aging physiology, Aging psychology, Environment, Hippocampus growth & development, Hippocampus physiology, Learning physiology, NF-kappa B metabolism, Neuronal Plasticity physiology
- Abstract
Aging weakens memory functions. Exposing healthy rodents or pathological rodent models to environmental enrichment (EE) housing improves their cognitive functions by changing neuronal levels of excitation, cellular signaling, and plasticity, notably in the hippocampus. At the molecular level, brain derived-neurotrophic factor (BDNF) represents an important player that supports EE-associated changes. EE facilitation of learning was also shown to correlate with chromatin acetylation in the hippocampus. It is not known, however, whether such mechanisms are still into play during aging. In this study, we exposed a cohort of aged rats (18-month-old) to either a 6 month period of EE or standard housing conditions and investigated chromatin acetylation-associated events [histone acetyltranferase activity, gene expression, and histone 3 (H3) acetylation] and epigenetic modulation of the Bdnf gene under rest conditions and during learning. We show that EE leads to upregulation of acetylation-dependent mechanisms in aged rats, whether at rest or following a learning challenge. We found an increased expression of Bdnf through Exon-I-dependent transcription, associated with an enrichment of acetylated H3 at several sites of Bdnf promoter I, more particularly on a proximal nuclear factor κB (NF-κB) site under learning conditions. We further evidenced p65/NF-κB binding to chromatin at promoters of genes important for plasticity and hippocampus-dependent learning (e.g., Bdnf, CamK2D). Altogether, our findings demonstrate that aged rats respond to a belated period of EE by increasing hippocampal plasticity, together with activating sustained acetylation-associated mechanisms recruiting NF-κB and promoting related gene transcription. These responses are likely to trigger beneficial effects associated with EE during aging., Significance Statement: Aging weakens memory functions. Optimizing the neuronal circuitry required for normal brain function can be achieved by increasing sensory, motor, and cognitive stimuli resulting from interactions with the environment (behavioral therapy). This can be experimentally modeled by exposing rodents to environmental enrichment (EE), as with large cages, numerous and varied toys, and interaction with other rodents. However, EE effects in aged rodents has been poorly studied, and it is not known whether beneficial mechanisms evidenced in the young adults can still be recruited during aging. Our study shows that aged rats respond to a belated period of EE by activating specific epigenetic and transcriptional signaling that promotes gene expression likely to facilitate plasticity and learning behaviors., (Copyright © 2016 the authors 0270-6474/16/364352-11$15.00/0.)
- Published
- 2016
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.