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9. EPS3.03 Effects of lumacaftor-ivacaftor therapy on CFTR function in Phe508del homozygous patients with cystic fibrosis

Author

Graeber, S.Y., primary, Dopfer, C., additional, Naehrlich, L., additional, Gyulumyan, L., additional, Scheuermann, H., additional, Hirtz, S., additional, Wege, S., additional, Mairbäurl, H., additional, Dorda, M., additional, Hyde, R., additional, Bagheri-Hanson, A., additional, Rueckes-Nilges, C., additional, Fischer, S., additional, Mall, M.A., additional, and Tümmler, B., additional

Published
2018
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11. Disparity Between Two-Dimensional Echocardiographic and Electroanatomic Left and Right Atrial Volumes in Patients Undergoing Catheter Ablation for Long-Standing Persistent Atrial Fibrillation

Author

ŠKŇOUŘIL, L., primary, HAVRÁNEK, Š., additional, BULKOVÁ, V., additional, DORDA, M., additional, PALEČEK, T., additional, ŠIMEK, J., additional, FINGROVÁ, Z., additional, LINHART, A., additional, JANUŠKA, J., additional, WICHTERLE, D., additional, and FIALA, M., additional

Published
2017
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13. Improving the radical cure of vivax malaria (IMPROV): a study protocol for a multicentre randomised, placebo-controlled comparison of short and long course primaquine regimens

Author

Abreha, T, Alemayehu, B, Assefa, A, Awab, GR, Baird, JK, Bezabih, B, Cheah, PY, Day, NP, Devine, A, Dorda, M, Dondorp, AM, Girma, S, Tran, TH, Jima, D, Kassa, M, Kebende, A, Khu, NH, Leslie, T, Ley, B, Lubell, Y, Mayan, I, Meaku, Z, Pasaribu, AP, Nguyen, HP, Price, RN, Simpson, JA, Solomon, H, Sutanto, I, Tadesse, Y, Taylor, B, Ngo, VT, Thriemer, K, von Seidlein, L, White, N, Woyessa, A, Yuentrakul, P, Zekria, R, Abreha, T, Alemayehu, B, Assefa, A, Awab, GR, Baird, JK, Bezabih, B, Cheah, PY, Day, NP, Devine, A, Dorda, M, Dondorp, AM, Girma, S, Tran, TH, Jima, D, Kassa, M, Kebende, A, Khu, NH, Leslie, T, Ley, B, Lubell, Y, Mayan, I, Meaku, Z, Pasaribu, AP, Nguyen, HP, Price, RN, Simpson, JA, Solomon, H, Sutanto, I, Tadesse, Y, Taylor, B, Ngo, VT, Thriemer, K, von Seidlein, L, White, N, Woyessa, A, Yuentrakul, P, and Zekria, R

Abstract

BACKGROUND: Plasmodium vivax malaria is a major cause of morbidity and recognised as an important contributor to mortality in some endemic areas. The current recommended treatment regimen for the radical cure of P. vivax includes a schizontocidal antimalarial, usually chloroquine, combined with a 14 day regimen of primaquine. The long treatment course frequently results in poor adherence and effectiveness. Shorter courses of higher daily doses of primaquine have the potential to improve adherence and thus effectiveness without compromising safety. The proposed multicentre randomised clinical trial aims to provide evidence across a variety of endemic settings on the safety and efficacy of high dose short course primaquine in glucose-6-phosphate-dehydrogenase (G6PD) normal patients. DESIGN: This study is designed as a placebo controlled, double blinded, randomized trial in four countries: Indonesia, Vietnam, Afghanistan and Ethiopia. G6PD normal patients diagnosed with vivax malaria are randomized to receive either 7 or 14 days high dose primaquine or placebo. G6PD deficient (G6PDd) patients are allocated to weekly primaquine doses for 8weeks. All treatment is directly observed and recurrent episodes are treated with the same treatment than allocated at the enrolment episode. Patients are followed daily until completion of treatment, weekly until 8 weeks and then monthly until 1 year after initiation of the treatment. The primary endpoint is the incidence rate (per person year) of symptomatic recurrent P. vivax parasitaemia over 12 months of follow-up, for all individuals, controlling for site, comparing the 7 versus 14-day primaquine treatment arms. Secondary endpoints are other efficacy measures such as incidence risk at different time points. Further endpoints are risks of haemolysis and severe adverse events. DISCUSSION: This study has been approved by relevant institutional ethics committees in the UK and Australia, and all participating countries. Results will b

Published
2015

16. 54 Intraclonal genome diversity of the major Pseudomonas aeruginosa clones C and PA14

Author

Cramer, N., primary, Fischer, S., additional, Losada, P.M., additional, Hilker, R., additional, Dethlefsen, S., additional, Dorda, M., additional, Munder, A., additional, Suerbaum, S., additional, Tamm, S., additional, Türk, O., additional, Woltemate, S., additional, Wiehlmann, L., additional, Chouvarine, P., additional, Klockgether, J., additional, and Tummler, B., additional

Published
2014
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19. Poster Session 1

Author

Deshmukh, A., primary, Sharma, S. S., additional, Gobal, F. G., additional, Singla, S. S., additional, Hebbar, P. H., additional, Paydak, H. P., additional, Igarashi, M., additional, Tada, H., additional, Sekiguchi, Y., additional, Yamasaki, H., additional, Kuroki, K., additional, Machino, T., additional, Yoshida, K., additional, Aonuma, K., additional, Shavadia, J., additional, Otieno, H., additional, Yonga, G., additional, Jinah, A., additional, Qvist, J. F., additional, Soerensen, P. H., additional, Dixen, U., additional, Ramirez-Marrero, M. A., additional, Perez-Villardon, B., additional, Gaitan-Roman, D., additional, Jimenez-Navarro, M., additional, Delgado-Prieto, J. L., additional, De Teresa-Galvan, E., additional, De Mora-Martin, M., additional, Deshmukh, A., additional, Hebbar, P. B., additional, Wei, W. X., additional, Bardari, S., additional, Zecchin, M., additional, Salame', R., additional, Vitali Serdoz, L., additional, Di Lenarda, A., additional, Guerrini, N., additional, Barbati, G., additional, Sinagra, G., additional, Hanazawa, K., additional, Kaitani, K., additional, Nakagawa, Y., additional, Lenaerts, I., additional, Driesen, R., additional, Hermida, N., additional, Heidbuchel, H., additional, Janssens, S., additional, Balligand, J. L., additional, Sipido, K. R., additional, Willems, R., additional, Sehra, R., additional, Krummen, D., additional, Briggs, C., additional, Narayan, S., additional, Tanaka, Y., additional, Hirao, K., additional, Nakamura, T., additional, Inaba, O., additional, Yagishita, A., additional, Higuchi, K., additional, Hachiya, H., additional, Isobe, M., additional, Kallergis, E., additional, Kanoupakis, E. M., additional, Mavrakis, H. E., additional, Goudis, C. A., additional, Maliaraki, N. E., additional, Vardas, P. E., additional, Kiuchi, K., additional, Piorkowski, C., additional, Kircher, S., additional, Gaspar, T., additional, Watanabe, N., additional, Bollmann, A., additional, Hindricks, G., additional, Wauters, K., additional, Grosse, A., additional, Raffa, S., additional, Brunelli, M., additional, Geller, J. C., additional, Maggioni, A. P., additional, Gonzini, L., additional, Gussoni, G., additional, Vescovo, G., additional, Gulizia, M., additional, Pirelli, S., additional, Mathieu, G., additional, Di Pasquale, G., additional, Salame, R., additional, Magnani, S., additional, Sakamoto, T., additional, Kumagai, K., additional, Fuke, E., additional, Nishiuchi, S., additional, Hayashi, T., additional, Miki, Y., additional, Naito, S., additional, Oshima, S., additional, Hof, I. E., additional, Vonken, E., additional, Velthuis, B. K., additional, Meine, M., additional, Hauer, R. N. W., additional, Loh, K. P., additional, Na, J. O., additional, Choi, C. U., additional, Kim, E. J., additional, Rha, S. W., additional, Park, C. G., additional, Seo, H. S., additional, Oh, D. J., additional, Lim, H. E., additional, Wichterle, D., additional, Bulkova, V., additional, Fiala, M., additional, Chovancik, J., additional, Simek, J., additional, Peichl, P., additional, Cihak, R., additional, Kautzner, J., additional, Glick, A., additional, Viskin, S., additional, Belhassen, B., additional, Navarrete, A., additional, Conte, F., additional, Ishti, A., additional, Sai, D., additional, Moran, M., additional, Chitovova, Z., additional, Ahmed, H., additional, Mares, K., additional, Skoda, J., additional, Sediva, L., additional, Petru, J., additional, Reddy, V. Y., additional, Neuzil, P., additional, Schmidt, M., additional, Dorwarth, U., additional, Leber, A., additional, Wankerl, M., additional, Krieg, J., additional, Straube, F., additional, Reif, S., additional, Hoffmann, E., additional, Mikhaylov, E., additional, Tikhonenko, V., additional, Lebedev, D., additional, Shin, S. Y., additional, Yong, H. S., additional, Choi, J. I., additional, Kim, S. H., additional, Matsuo, S., additional, Yamane, T., additional, Hioki, M., additional, Ito, K., additional, Narui, R., additional, Date, T., additional, Sugimoto, K., additional, Yoshimura, M., additional, Rolf, S., additional, Sommer, P., additional, Batalov, R., additional, Popov, S., additional, Antonchenko, I., additional, Suslova, T., additional, Fichtner, S., additional, Czudnochowsky, U., additional, Estner, H. L., additional, Ammar, S., additional, Reents, T., additional, Jilek, C., additional, Hessling, G., additional, Deisenhofer, I., additional, Pokushalov, E., additional, Romanov, A., additional, Corbucci, G., additional, Artemenko, S., additional, Losik, D., additional, Shabanov, V., additional, Turov, A., additional, Elesin, D., additional, Abramov, M., additional, Sanders, P., additional, Jais, P., additional, Roberts-Thomson, K., additional, Fukumoto, K., additional, Takatsuki, S., additional, Kimura, T., additional, Nishiyama, N., additional, Aizawa, Y., additional, Sato, T., additional, Miyoshi, S., additional, Fukuda, K., additional, Roux, Y., additional, Tenkorang, J., additional, Carroz, P., additional, Schlaepfer, J., additional, Pascale, P., additional, Forclaz, A., additional, Fromer, M., additional, Pruvot, E., additional, Sknouril, L., additional, Nevralova, R., additional, Dorda, M., additional, Januska, J., additional, Santi, R., additional, Geller, C., additional, Nakamura, K., additional, Kasseno, K., additional, Taniguchi, K., additional, Wutzler, A., additional, Huemer, M., additional, Parwani, A., additional, Boldt, L. H., additional, Blaschke, D., additional, Dietz, R., additional, Haverkamp, W., additional, Coutu, B., additional, Malanuk, R., additional, Ait Said, M., additional, Vicentini, A., additional, Schade, S., additional, Ando, K., additional, Rousseauplasse, A., additional, Deering, T., additional, Picarra, B. C., additional, Santos, A. R., additional, Dionisio, P., additional, Semedo, P., additional, Matos, R., additional, Leitao, M., additional, Jacinto, A., additional, Trinca, M., additional, Wan, C., additional, Glad, J., additional, Szymkiewicz, S., additional, Habibovic, M., additional, Versteeg, H., additional, Pelle, A. J. M., additional, Theuns, D. A. M. J., additional, Jordaens, L., additional, Pedersen, S. S., additional, Pakarinen, S., additional, Toivonen, L., additional, Taggeselle, J., additional, Frey, A., additional, Birkenhagen, A., additional, Kohler, S., additional, Maier, S. K. G., additional, Lobitz, N., additional, Paule, S., additional, Becher, J., additional, Mustafa, G., additional, Ibrahim, A., additional, King, G., additional, Foley, B., additional, Wilkoff, B., additional, Freedman, R., additional, Hayes, D., additional, Kalbfleisch, S., additional, Kutalek, S., additional, Schaerf, R., additional, Fazal, I. A., additional, Tynan, M., additional, Plummer, C. J., additional, Mccomb, J. M., additional, Oto, A., additional, Aytemir, K., additional, Yorgun, H., additional, Canpolat, U., additional, Kaya, E. B., additional, Tokgozoglu, L., additional, Kabakci, G., additional, Ozkutlu, H., additional, Greenberg, S., additional, Hamati, F., additional, Styperek, R., additional, Alonso, J., additional, Peress, D., additional, Bolanos, O., additional, Augostini, R., additional, Pelini, M., additional, Zhang, S., additional, Stoycos, S., additional, Witsaman, S., additional, Mowrey, K., additional, Bremer, J., additional, Oza, A., additional, Ciconte, G., additional, Mazzone, P., additional, Paglino, G., additional, Marzi, A., additional, Vergara, P., additional, Sora, N., additional, Gulletta, S., additional, Della Bella, P., additional, Nagashima, M., additional, Goya, M., additional, Soga, Y., additional, Hiroshima, K., additional, Andou, K., additional, Hayashi, K., additional, An, Y., additional, Nobuyoshi, M., additional, Kutarski, A., additional, Malecka, B., additional, Pietura, R., additional, Osmancik, P., additional, Herman, D., additional, Stros, P., additional, Kocka, V., additional, Tousek, P., additional, Linkova, H., additional, Bortnik, M., additional, Occhetta, E., additional, Dell'era, G., additional, Degiovanni, A., additional, Plebani, L., additional, Marino, P. N., additional, Gorev, M. V., additional, Alimov, D. G., additional, Raju, P., additional, Kully, S., additional, Ugni, S., additional, Furniss, S., additional, Lloyd, G., additional, Patel, N. R., additional, Richards, M. W., additional, Warren, C. E., additional, Anderson, M. H., additional, Hero, M., additional, Rey, J. L., additional, Ouali, S., additional, Azzez, S., additional, Kacem, S., additional, Hammas, S., additional, Ben Salem, H., additional, Neffeti, E., additional, Remedi, F., additional, Boughzela, E., additional, Kronborg, M. B., additional, Mortensen, P. T., additional, Poulsen, S. H., additional, Nielsen, J. C., additional, Simantirakis, E. N., additional, Kontaraki, J. E., additional, Arkolaki, E. G., additional, Chrysostomakis, S. I., additional, Nyktari, E. G., additional, Patrianakos, A. P., additional, Funck, R. C., additional, Harink, C., additional, Mueller, H. H., additional, Koelsch, S., additional, Maisch, B., additional, Bolzani, V., additional, Costandi, P., additional, Shehada, R. E., additional, Butala, N., additional, Coppola, B., additional, Taborsky, M., additional, Heinc, P., additional, Fedorco, M., additional, Doupal, V., additional, Di Cori, A., additional, Zucchelli, G., additional, Soldati, E., additional, Segreti, L., additional, De Lucia, R., additional, Viani, S., additional, Paperini, L., additional, Bongiorni, M. G., additional, Gutleben, K. J., additional, Kranig, W., additional, Barr, C., additional, Morgenstern, M. M., additional, Simon, M., additional, Dalal, Y. H., additional, Landolina, M., additional, Pierantozzi, A., additional, Agricola, T., additional, Lunati, M., additional, Pisano', E., additional, Lonardi, G., additional, Bardelli, G., additional, Zucchi, G., additional, Thibault, B., additional, Dubuc, M., additional, Karst, E., additional, Ryu, K., additional, Paiement, P., additional, Carlson, M. D., additional, Farazi, T., additional, Alhous, H., additional, Mont, L., additional, Porres, J. M., additional, Alzueta, J., additional, Beiras, X., additional, Fernandez-Lozano, I., additional, Macias, A., additional, Ruiz, R., additional, Brugada, J., additional, Viani, S. M., additional, Seifert, M., additional, Schau, T., additional, Moeller, V., additional, Meyhoefer, J., additional, Butter, C., additional, Ganiere, V., additional, Niculescu, V., additional, Domenichini, G., additional, Stettler, C., additional, Defaye, P., additional, Burri, H., additional, Stockburger, M., additional, De Teresa, E., additional, Lamas, G., additional, Desaga, M., additional, Koenig, C., additional, Cobo, E., additional, Navarro, X., additional, Wiegand, U., additional, Blich, M., additional, Carasso, S., additional, Suleiman, M., additional, Marai, I., additional, Gepstein, L., additional, Boulos, M., additional, Sasov, M., additional, Liska, B., additional, Margitfalvi, P., additional, Malacky, T., additional, Svetlosak, M., additional, Goncalvesova, E., additional, Hatala, R., additional, Takaya, Y., additional, Noda, T., additional, Yamada, Y., additional, Okamura, H., additional, Satomi, K., additional, Shimizu, W., additional, Aihara, N., additional, Kamakura, S., additional, Proclemer, A., additional, Boveda, S., additional, Oswald, H., additional, Scipione, P., additional, Da Costa, A., additional, Brzozowski, W., additional, Tomaszewski, A., additional, Wysokinski, A., additional, Arbelo, E., additional, Tamborero, D., additional, Vidal, B., additional, Tolosana, J. M., additional, Sitges, M., additional, Matas, M., additional, Botto, G. L., additional, Dicandia, C. D., additional, Mantica, M., additional, La Rosa, C., additional, D' Onofrio, A., additional, Molon, G., additional, Raciti, G., additional, Verlato, R., additional, Foley, P. W. X., additional, Chalil, S., additional, Ratib, K., additional, Smith, R. E. A., additional, Printzen, F., additional, Auricchio, A., additional, Leyva, F., additional, Abu Sham'a, R., additional, Buber, J., additional, Luria, D., additional, Kuperstein, R., additional, Feinberg, M., additional, Granit, H., additional, Eldar, M., additional, Glikson, M., additional, Vondrak, K., additional, Nof, E., additional, Lipchenca, I., additional, Vatasescu, R.- G., additional, Iorgulescu, C., additional, Caldararu, C., additional, Vasile, A., additional, Bogdan, S., additional, Constantinescu, D., additional, Dorobantu, M., additional, Sakaguchi, H., additional, Miyazaki, A., additional, Yamamoto, T., additional, Fujimoto, K., additional, Ono, S., additional, Ohuchi, H., additional, Martinelli, M., additional, Martins, S., additional, Molina, R., additional, Siqueira, S., additional, Nishioka, S. A. D., additional, Peixoto, G. L., additional, Alkmim-Teixeira, R., additional, Costa, R., additional, Meine, M. M., additional, Tuinenburg, A. E., additional, Doevendans, P. A., additional, Denollet, J., additional, Goscinska-Bis, K., additional, Zupan, I., additional, Van Der, H., additional, Anselme, F., additional, Hartog, H., additional, Block, M., additional, Borri, A., additional, Padeletti, L., additional, Toniolo, M., additional, Zanotto, G., additional, Rossi, A., additional, Raytcheva, E., additional, Tomasi, L., additional, Vassanelli, C., additional, Fernandez Lozano, I., additional, Mitroi, C., additional, Toquero Ramos, J., additional, Castro Urda, V., additional, Monivas Palomero, V., additional, Corona Figueroa, A., additional, Ruiz Bautista, L., additional, Alonso Pulpon, L., additional, Jadidi, A. S., additional, Sacher, F., additional, Shah, A. S., additional, Scherr, D., additional, Derval, N., additional, Hocini, M., additional, Haissaguerre, M., additional, Castrejon Castrejon, S., additional, Largo-Aramburu, C., additional, Sachar, J., additional, Gang, E., additional, Estrada, A., additional, Doiny, D., additional, De Miguel, E., additional, Merino, J. L., additional, Trevisi, N., additional, Ricco, A., additional, Petracca, F., additional, Baratto, F., additional, Bisceglie, A., additional, Maccabelli, G., additional, El-Damaty, A., additional, Sapp, J., additional, Warren, J., additional, Macinnis, P., additional, Horacek, M., additional, Dinov, B., additional, Schoenbauer, R., additional, Braunschweig, F., additional, Arya, A., additional, Andreu, D., additional, Berruezo, A., additional, Ortiz, J. T., additional, Silva, E., additional, De Caralt, T. M., additional, Fernandez-Armenta, J., additional, Perez-Silva, A., additional, Ortega, M., additional, Lopez-Sendon, J. L., additional, Regoli, F., additional, Faletra, F., additional, Nucifora, G., additional, Pasotti, E., additional, Moccetti, T., additional, Klersy, C., additional, Casella, M., additional, Dello Russo, A., additional, Moltrasio, M., additional, Zucchetti, M., additional, Fassini, G., additional, Di Biase, L., additional, Natale, A., additional, Tondo, C., additional, Matsuhashi, N., additional, Weig, H. J., additional, Kerst, G., additional, Weretk, S., additional, Seizer, P., additional, Gawaz, M. P., additional, Schreieck, J., additional, Sarquella-Brugada, G., additional, Prada, F., additional, Salling, C. M., additional, Kolb, C., additional, Pytkowski, M., additional, Maciag, A., additional, Farkowski, M., additional, Jankowska, A., additional, Kowalik, I., additional, Kraska, A., additional, Szwed, H., additional, Maury, P., additional, Duparc, A., additional, Mondoly, P., additional, Rollin, A., additional, Pap, R., additional, Kohari, M., additional, Bencsik, G., additional, Makai, A., additional, Saghy, L., additional, Forster, T., additional, Ebrille, E., additional, Scaglione, M., additional, Raimondo, C., additional, Caponi, D., additional, Di Donna, P., additional, Blandino, A., additional, Delcre, S. D. L., additional, Gaita, F., additional, Roca Luque, I., additional, Dos, L. D. S., additional, Rivas, N. R. G., additional, Pijuan, A. P. D., additional, Perez, J., additional, Casaldaliga, J., additional, Garcia-Dorado, D. G. D., additional, Moya, A. M. M., additional, Sato, H., additional, Yagi, T., additional, Yambe, T., additional, Streitner, F., additional, Dietrich, C., additional, Mahl, E., additional, Schoene, N., additional, Veltmann, C., additional, Borggrefe, M., additional, Kuschyk, J., additional, Sadarmin, P. P., additional, Wong, K. C. K., additional, Rajappan, K., additional, Bashir, Y., additional, Betts, T. R., additional, Leclercq, C., additional, Martins, R., additional, Daubert, J. C., additional, Mabo, P., additional, Koide, M., additional, Hamano, G., additional, Taniguchi, T., additional, Yamato, M., additional, Sasaki, N., additional, Hirooka, K., additional, Ikeda, Y., additional, Yasumura, Y., additional, Dichtl, W., additional, Wolber, T., additional, Paoli, U., additional, Bruellmann, S., additional, Berger, T., additional, Stuehlinger, M., additional, Duru, F., additional, Hintringer, F., additional, Kanoupakis, E., additional, Mavrakis, H., additional, Koutalas, E., additional, Saloustros, I., additional, Goudis, C., additional, Chlouverakis, G., additional, Vardas, P., additional, Herre, J. M., additional, Saeed, M., additional, Saberi, L., additional, Neuman, S., additional, Yamaji, K., additional, Iwabuchi, M., additional, Baranchuk, A., additional, Femenia, F., additional, Miranda Hermosilla, R., additional, Lopez Diez, J. C., additional, Serra, J. L., additional, Valentino, M., additional, Retyk, E., additional, Galizio, N., additional, Kwasniewski, W., additional, Filipecki, A., additional, Orszulak, W., additional, Urbanczyk-Swic, D., additional, Trusz - Gluza, M., additional, Piot, O., additional, Degand, B., additional, Donofrio, A., additional, Scanu, P., additional, Quesada, A., additional, Kloppe, A., additional, Mijic, D., additional, Bogossian, H., additional, Zarse, M., additional, Lemke, B., additional, Tyler, J., additional, Comfort, G., additional, Deering, T. F., additional, Epstein, A. E., additional, Greenberg, S. M. G., additional, Goldman, D. S., additional, Rhude, J., additional, Majewski, J. P., additional, Lelakowski, J., additional, Tomala, I., additional, Santos, C. M., additional, Miranda, R. S., additional, Sousa, P. J., additional, Cavaco, D. M., additional, Adragao, P. P., additional, Knops, R. E., additional, Wilde, A. A., additional, Belhameche, M., additional, Hermida, J. S., additional, Dovellini, E., additional, Frohlig, G., additional, Siot, P., additional, Duray, G. Z., additional, Israel, C. W., additional, Brachmann, J., additional, Seidl, K. H., additional, Foresti, M., additional, Birkenhauer, F., additional, Hohnloser, S. H., additional, Ferreira, C., additional, Mateus, P., additional, Ribeiro, H., additional, Carvalho, S., additional, Ferreira, A., additional, Moreira, J., additional, Kadro, W., additional, Rahim, H., additional, Turkmani, M., additional, Abu Lebdeh, M., additional, Altabban, A., additional, Cerrato, N., additional, Rivera, S., additional, Scazzuso, F., additional, Albina, G., additional, Klein, A., additional, Laino, R., additional, Sammartino, V., additional, Giniger, A., additional, Kvantaliani, T., additional, Akhvlediani, M., additional, Namdar, M., additional, Steffel, J., additional, Jetzer, S., additional, Bayrak, F., additional, Chierchia, G. B., additional, Jenni, R., additional, Brugada, P., additional, Bakos, Z., additional, Medvedev M, M. M., additional, Jonas Carlsson, J. C., additional, Fredrik Holmqvist, F. H., additional, Pyotr Platonov, P. P., additional, Nurbaev, T., additional, Pirnazarov, M., additional, Nikishin, A., additional, Aagaard, P., additional, Sahlen, A., additional, Bergfeldt, L., additional, Simeonidou, E., additional, Kastellanos, S., additional, Varounis, C., additional, Michalakeas, C., additional, Koniari, C., additional, Nikolopoulou, A., additional, Anastasiou-Nana, M., additional, Furukawa, Y., additional, Yamada, T., additional, Morita, T., additional, Tanaka, K., additional, Iwasaki, Y., additional, Kawasaki, M., additional, Kuramoto, Y., additional, Fukunami, M., additional, Blanche, C., additional, Tran, N., additional, Rigamonti, F., additional, Zimmermann, M., additional, Okisheva, E., additional, Tsaregorodtsev, D., additional, Sulimov, V., additional, Novikova, D., additional, Popkova, T., additional, Udachkina, E., additional, Korsakova, Y., additional, Volkov, A., additional, Novikov, A., additional, Alexandrova, E., additional, Nasonov, E., additional, Arsenos, P., additional, Gatzoulis, K., additional, Manis, G., additional, Dilaveris, P., additional, Gialernios, T., additional, Kartsagoulis, E., additional, Asimakopoulos, S., additional, Stefanadis, C., additional, Marocolo, M., additional, Barbosa Neto, O., additional, Carvalho, A. C., additional, Marques Neto, S. R., additional, Mota, G. R., additional, Barbosa, P. R. B., additional, Fernandez-Fernandez, A., additional, Manzano Fernandez, S., additional, Pastor-Perez, F. J., additional, Barquero-Perez, O., additional, Goya-Esteban, R., additional, Salar, M., additional, Rojo-Alvarez, J. L., additional, Garcia-Alberola, A., additional, Takigawa, M., additional, Kawamura, M., additional, Aiba, T., additional, Sakaguchi, T., additional, Itoh, H., additional, Horie, M., additional, Igarashi, T., additional, Negishi, J., additional, Toyota, N., additional, Yamada, O., additional, Papavasileiou, M., additional, Cabrera Bueno, F., additional, Molina Mora, M. J., additional, Alzueta Rodriguez, J., additional, Barrera Cordero, A., additional, De Teresa Galvan, E., additional, Revishvili, A. S., additional, Dzhordzhikiya, T., additional, Sopov, O., additional, Simonyan, G., additional, Lyadzhina, O., additional, Fetisova, E., additional, Kalinin, V., additional, Balt, J. C., additional, Steggerda, R. C., additional, Boersma, L. V. A., additional, Wijffels, M. C. E. F., additional, Wever, E. F. D., additional, Ten Berg, J. M., additional, Ricci, R. P., additional, Morichelli, L., additional, D'onofrio, A., additional, Vaccari, D., additional, Calo', L., additional, Buja, G., additional, Rovai, N., additional, Gargaro, A., additional, Sperzel, J., additional, Speca, G., additional, Santini, L., additional, Haarbo, J., additional, Dubin, K., additional, Carlson, M., additional, Garcia Quintana, A., additional, Mendoza-Lemes, H., additional, Garcia Perez, L., additional, Led Ramos, S., additional, Caballero Dorta, E., additional, Matinez De Espronceda, M., additional, Piro Mastracchio, V., additional, Serrano Arriezu, L., additional, Sciarra, L., additional, Marziali, M., additional, Marras, E., additional, Rebecchi, M., additional, Allocca, G., additional, Lioy, E., additional, Delise, P., additional, Santobuono, V. E., additional, Iacoviello, M., additional, Nacci, F., additional, Luzzi, G., additional, Puzzovivo, A., additional, Memeo, M., additional, Quadrini, F., additional, Favale, S., additional, Trucco, M. E., additional, Arce, M., additional, Palazzolo, J., additional, Uribe, W., additional, Maggi, R., additional, Furukawa, T., additional, Croci, F., additional, Solano, A., additional, Brignole, M., additional, Lebreiro, A., additional, Sousa, A., additional, Correia, A. S., additional, Lourenco, P., additional, Oliveira, S., additional, Paiva, M., additional, Freitas, J., additional, Maciel, M. J., additional, Linker, N., additional, Rieger, G., additional, Garutti, C., additional, Edvardsson, N., additional, Salguero Bodes, R., additional, De Riva Silva, M., additional, Fontenla Cerezuela, A., additional, Lopez Gil, M., additional, Mejia Martinez, E., additional, Jurado Roman, A., additional, Garcia Alvarez, S., additional, Arribas Ynsaurriaga, F., additional, Petix, N. R., additional, Del Rosso, A., additional, Guarnaccia, V., additional, Zipoli, A., additional, Rabajoli, F., additional, Foglia Manzillo, G., additional, Tolardo, C., additional, Checchinato, C., additional, Chiaravallotti, S., additional, Santarone, M., additional, Spinnler, M. T., additional, Podoleanu, C., additional, Frigy, A., additional, Dobreanu, D., additional, Ginghina, C., additional, and Carasca, E., additional

Published
2011
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20. Poster session V * Saturday 11 December 2010, 08:30-12:30

Author

Pham, Q. H., primary, Von Lueder, T. G., additional, Namtvedt, S. K., additional, Rosjo, H., additional, Omland, T., additional, Steine, K., additional, Timoteo, A. T., additional, Mota Carmo, M., additional, Simoes, M., additional, Branco, L. M., additional, Ferreira, R. C., additional, Kato, R., additional, Ito, J., additional, Tahara, T., additional, Yokoyama, Y., additional, Ashikaga, T., additional, Satoh, Y., additional, Na, J. O., additional, Hong, H. E., additional, Kim, M. N., additional, Shin, S. Y., additional, Choi, C. U., additional, Kim, E. J., additional, Rha, S. W., additional, Park, C. G., additional, Seo, H. S., additional, Oh, D. J., additional, Ticulescu, R., additional, Brigido, S., additional, Vriz, O., additional, Sparacino, L., additional, Popescu, B. A., additional, Ginghina, C., additional, Carerj, S., additional, Nicolosi, G. L., additional, Antonini-Canterin, F., additional, Onaindia Gandarias, J. J., additional, Romero, A., additional, Laraudogoitia, E., additional, Velasco, S., additional, Quintana, O., additional, Cacicedo, A., additional, Rodriguez, I., additional, Alarcon, J. A., additional, Gonzalez, J., additional, Lekuona, I., additional, Subinas, A., additional, Abdula, G., additional, Lund, L. H., additional, Winter, R., additional, Brodin, L., additional, Sahlen, A., additional, Masaki, M., additional, Cha, Y. M., additional, Yuasa, T., additional, Dong, K., additional, Dong, Y. X., additional, Mankad, S. V., additional, Oh, J. K., additional, Vallet, F., additional, Lequeux, B., additional, Diakov, C., additional, Sosner, P., additional, Christiaens, L., additional, Coisne, D., additional, Kihara, C., additional, Murata, K., additional, Wada, Y., additional, Uchida, K., additional, Ueyama, T., additional, Okuda, S., additional, Susa, T., additional, Matsuzaki, M., additional, Cho, E. J., additional, Choi, K. Y., additional, Kwon, B. J., additional, Kim, D. B., additional, Jang, S. W., additional, Cho, J. S., additional, Jung, H. O., additional, Jeon, H. K., additional, Youn, H. J., additional, Kim, J. H., additional, Cikes, M., additional, Bijnens, B., additional, Velagic, V., additional, Kopjar, T., additional, Milicic, D., additional, Biocina, B., additional, Gasparovic, H., additional, Almuntaser, I., additional, Brown, A., additional, Foley, B., additional, Mulvihill, N., additional, Crean, P., additional, King, G., additional, Murphy, R., additional, Takata, Y., additional, Taniguchi, M., additional, Nobusada, S., additional, Sugawara, M., additional, Toh, N., additional, Kusano, K., additional, Itoh, H., additional, Wellnhofer, E., additional, Kriatselis, C., additional, Nedios, S., additional, Gerds-Li, J. H., additional, Fleck, E., additional, Poulsen, M. K., additional, Henriksen, J. E., additional, Dahl, J., additional, Johansen, A., additional, Haghfelt, T., additional, Hoilund-Carlsen, P. F., additional, Beck-Nielsen, H., additional, Moller, J. E., additional, Dankowski, R., additional, Wierzchowiecki, M., additional, Michalski, M., additional, Nowicka, A., additional, Szymanowska, K., additional, Pajak, A., additional, Poprawski, K., additional, Szyszka, A., additional, Kasner, M., additional, Westermann, D., additional, Schultheiss, H. P., additional, Tschoepe, C., additional, Watanabe, T., additional, Iwai-Takano, M., additional, Kobayashi, A., additional, Machii, H., additional, Takeishi, Y., additional, Paelinck, B. P., additional, Van Herck, P. L., additional, Bosmans, J. M., additional, Vrints, C. J., additional, Lamb, H. J., additional, Doltra, A., additional, Vidal, B., additional, Silva, E., additional, Poyatos, S., additional, Mont, L., additional, Berruezo, A., additional, Castel, A., additional, Tolosana, J. M., additional, Brugada, J., additional, Sitges, M., additional, Dencker, M., additional, Bjorgell, O., additional, Hlebowicz, J., additional, Szelenyi, Z. S., additional, Szenasi, G., additional, Kiss, M., additional, Prohaszka, Z., additional, Patocs, A., additional, Karadi, I., additional, Vereckei, A., additional, Saha, S. K., additional, Anderson, P. L., additional, Govind, S., additional, Govindan, M., additional, Moggridge, J. C., additional, Kiotsekoglou, A., additional, Gopal, A. S., additional, Loegstrup, B. B., additional, Christophersen, T. B., additional, Hoefsten, D. E., additional, Moeller, J. E., additional, Boetker, H. E., additional, Egstrup, K., additional, Graefe, M., additional, Huang, F. Q., additional, Zhang, R. S., additional, Le, T. T., additional, Tan, R. S., additional, Sattarzadeh Badkoubeh, R., additional, Tavoosi, A., additional, Elahian, A. R., additional, Drapkina, O., additional, Ivashkin, V. I., additional, Fazakas, A., additional, Pepo, L., additional, Janosi, O., additional, Kopitovic, I., additional, Goncalves, A., additional, Marcos-Alberca, P., additional, Almeria, C., additional, Feltes, G., additional, Rodriguez, E., additional, Garcia, E., additional, Hernandez-Antolin, R., additional, Macaya, C., additional, Silva Cardoso, J., additional, Zamorano, J. L., additional, Navarro, M. S., additional, Valentin, M., additional, Banes, C. M., additional, Rigo, F., additional, Grolla, E., additional, Tona, F., additional, Cuaia, V., additional, Moreo, A., additional, Badano, L., additional, Raviele, A., additional, Iliceto, S., additional, Tarzia, P., additional, Sestito, A., additional, Nerla, R., additional, Di Monaco, A., additional, Infusino, F., additional, Matera, D., additional, Greco, F., additional, Tacchino, R. M., additional, Lanza, G. A., additional, Crea, F., additional, Nemes, A., additional, Balazs, E., additional, Pinter, K. S., additional, Egyed, A., additional, Csanady, M., additional, Forster, T., additional, Holte, E., additional, Vegsundvag, J., additional, Hole, T., additional, Hegbom, K., additional, Wiseth, R., additional, Sharif, D., additional, Sharif-Rasslan, A., additional, Shahla, C., additional, Khalil, A., additional, Rosenschein, U., additional, Zagatina, A., additional, Zhuravskaya, N., additional, Tyurina, T. V., additional, Tagliamonte, E., additional, Cirillo, T., additional, Coppola, A., additional, Marinelli, U., additional, Romano, C., additional, Riccio, G., additional, Citro, R., additional, Astarita, C., additional, Capuano, N., additional, Quaranta, G., additional, Desiderio, A., additional, Frattini, S., additional, Faggiano, P., additional, Zilioli, V., additional, Locantore, E., additional, Longhi, S., additional, Bellandi, F., additional, Faden, G., additional, Triggiani, M., additional, Dei Cas, L., additional, Dalsgaard, M., additional, Kjaergaard, J., additional, Iversen, K., additional, Hassager, C., additional, Dinh, W., additional, Nickl, W. N., additional, Smettan, J. S., additional, Koehler, T. K., additional, Scheffold, T. D., additional, Coll Barroso, M. C. B., additional, Guelker, J. G., additional, Fueth, R. F., additional, Kamperidis, V., additional, Hadjimiltiades, S., additional, Sianos, G., additional, Efthimiadis, G., additional, Karvounis, H., additional, Parcharidis, G., additional, Styliadis, I. H., additional, Velasco Del Castillo, M. S., additional, Onaindia, J. J., additional, Telleria, M., additional, Carstensen, H. G., additional, Nordenberg, C., additional, Sogaard, P., additional, Fritz-Hansen, T., additional, Bech, J., additional, Galatius, S., additional, Jensen, J. S., additional, Mogelvang, R., additional, Bartko, P. E., additional, Graf, S., additional, Rosenhek, R., additional, Burwash, I. G., additional, Bergler-Klein, J., additional, Clavel, M.-A., additional, Baumgartner, H., additional, Pibarot, P., additional, Mundigler, G., additional, Kirilmaz, B., additional, Eser, I., additional, Tuzun, N., additional, Komur, B., additional, Dogan, H., additional, Taskiran Comez, A., additional, Ercan, E., additional, Cusma-Piccione, M., additional, Zito, C., additional, Oreto, G., additional, Piluso, S., additional, Tripepi, S., additional, Oreto, L., additional, Longordo, C., additional, Ciraci, L., additional, Di Bella, G., additional, Piatkowski, R., additional, Kochanowski, J., additional, Scislo, P., additional, Grabowski, M., additional, Marchel, M., additional, Roik, M., additional, Kosior, D., additional, Opolski, G., additional, Sknouril, L., additional, Dorda, M., additional, Holek, B., additional, Gajdusek, L., additional, Chovancik, J., additional, Branny, M., additional, Fiala, M., additional, Szymanski, P., additional, Lipczynska, M., additional, Klisiewicz, A., additional, Hoffman, P., additional, Jander, N., additional, Minners, J., additional, Martin, G., additional, Zeh, W., additional, Allgeier, M., additional, Gohlke-Baewolf, C., additional, Gohlke, H., additional, Nistri, S., additional, Porciani, M. C., additional, Attanasio, M., additional, Abbate, R., additional, Gensini, G. F., additional, Pepe, G., additional, Duncan, R. F., additional, Piantadosi, C., additional, Nelson, A. J., additional, Wittert, G., additional, Dundon, B., additional, Worthley, M. I., additional, Worthley, S. G., additional, Jung, P., additional, Berlinger, K., additional, Rieber, J., additional, Sohn, H. Z., additional, Schneider, P., additional, Leibig, M., additional, Koenig, A., additional, Klauss, V., additional, Tomkiewicz-Pajak, L., additional, Kolcz, J., additional, Olszowska, M., additional, Pieculewicz, M., additional, Podolec, P., additional, Przewlocki, T., additional, Suchon, E., additional, Sobien, B., additional, Wilkolek, P., additional, Ziembicka, A., additional, Hlawaty, M., additional, Van De Bruaene, A., additional, Hermans, H., additional, Buys, R., additional, Vanhees, L., additional, Delcroix, M., additional, Voigt, J.-U., additional, Budts, W., additional, De Cillis, E., additional, Acquaviva, T., additional, Basile, D., additional, Bortone, A. S., additional, Kalimanovska-Ostric, D., additional, Nastasovic, T., additional, Vujisic-Tesic, B., additional, Jovanovic, I., additional, Milakovic, B., additional, Dostanic, M., additional, Stosic, M., additional, Frogoudaki, A., additional, Andreou, K., additional, Parisis, J., additional, Triantafyllidi, E., additional, Gaitani, S., additional, Paraskevaidis, J., additional, Anastasiou-Nana, M., additional, De Pasquale, G., additional, Kuehn, A., additional, Petzuch, K., additional, Mueller, J., additional, Meierhofer, C., additional, Fratz, S., additional, Hager, A., additional, Hess, J., additional, Vogt, M., additional, Attenhofer Jost, C. H., additional, Dearani, J. A., additional, Scott, C. G., additional, Burkhart, H. M., additional, Connolly, H. M., additional, Vitarelli, A., additional, Battaglia, D., additional, Caranci, F., additional, Padella, V., additional, Continanza, G., additional, Dettori, O., additional, Capotosto, L., additional, Vitarelli, M., additional, De Cicco, V., additional, Cortez Morichetti, M., additional, Mohanan Nair, K. K., additional, Sasidaharan, B., additional, Thajudeen, A., additional, Tharakan, J. M., additional, Mertens, L., additional, Ahmad, N., additional, Kantor, P. K., additional, Grosse-Wortmann, L., additional, Friedberg, M. K., additional, Bernard, Y. F., additional, Morel, M. A., additional, Descotes-Genon, V., additional, Jehl, J., additional, Meneveau, N., additional, Schiele, F., additional, Kaldararova, M., additional, Simkova, I., additional, Tittel, P., additional, Masura, J., additional, Trojnarska, O., additional, Szczepaniak, L., additional, Mizia -Stec, K., additional, Cieplucha, A., additional, Bartczak, A., additional, Grajek, S., additional, Tykarski, A., additional, Gasior, Z., additional, Babovicvuksanovic, D., additional, Bonnichsen, C. R., additional, Morgan, G. J., additional, Slorach, C., additional, Hui, W., additional, Sarkola, T., additional, Lee, K. J., additional, Chaturvedi, R., additional, Benson, L., additional, Bradley, T., additional, Iancu, M. E., additional, Ghiorghiu, I., additional, Serban, M., additional, Craciunescu, I., additional, Hodo, A., additional, Morgan, J., additional, Roche, L., additional, Lee, K., additional, Milanesi, O., additional, Favero, V., additional, Padalino, M., additional, Biffanti, R., additional, Cerutti, A., additional, Maschietto, N., additional, Reffo, E., additional, Vida, V., additional, Stellin, G., additional, Irtyuga, O., additional, Gamazin, D., additional, Voronkina, I., additional, Tsoyi, N., additional, Gudkova, E., additional, Moiseeva, O., additional, Aggeli, C., additional, Kazazaki, C., additional, Felekos, I., additional, Lagoudakou, S., additional, Roussakis, G., additional, Skoumas, J., additional, Pitsavos, C., additional, Stefanadis, C., additional, Cueff, C., additional, Keenan, N., additional, Steg, P. G., additional, Cimadevilla, C., additional, Ducrocq, G., additional, Vahanian, A., additional, Messika-Zeitoun, D., additional, Petrella, L., additional, Mazzola, A. M., additional, Villani, C. V., additional, Giancola, R. G., additional, Ciocca, M. C., additional, Di Eusanio, D. E. M., additional, Nolan, S., additional, Ionescu, A., additional, Skaug, T. R., additional, Amundsen, B. H., additional, Hergum, T., additional, Torp, H., additional, Haugen, B. O., additional, Lopez Aguilera, J., additional, Mesa Rubio, D., additional, Ruiz Ortiz, M., additional, Delgado Ortega, M., additional, Villanueva Fernandez, E., additional, Cejudo Diaz Del Campo, L., additional, Toledano Delgado, F., additional, Leon Del Pino, M., additional, Romo Pena, E., additional, Suarez De Lezo Cruz-Conde, J., additional, De Marco, E., additional, Colucci, A., additional, Comerci, G., additional, Gabrielli, F. A., additional, Natali, R., additional, Garramone, B., additional, Savino, M., additional, Lotrionte, M., additional, Sonaglioni, A., additional, Loperfido, F., additional, Zdravkovic, M., additional, Perunicic, J., additional, Krotin, M., additional, Ristic, M., additional, Vukomanovic, V., additional, Zaja, M., additional, Radovanovic, S., additional, Saric, J., additional, Zdravkovic, D., additional, Cotrim, C., additional, Almeida, A. R., additional, Miranda, R., additional, Almeida, A. G., additional, Picano, E., additional, Carrageta, M., additional, D'andrea, A., additional, Cocchia, R., additional, Riegler, L., additional, Golia, E., additional, Scarafile, R., additional, Caso, P., additional, Russo, M. G., additional, Bossone, E., additional, Calabro', R., additional, Noman, H., additional, Adel, A., additional, Elfaramawy, A. M. R., additional, Abdelraouf, M., additional, Elnaggar, W. A. E. L., additional, Baligh, E., additional, Sargento, L., additional, Silva, D., additional, Goncalves, S., additional, Ribeiro, S., additional, Vinhas Sousa, G., additional, Almeida, A., additional, Lopes, M., additional, Rodriguez-Manero, M., additional, Aguado Gil, L., additional, Azcarate, P., additional, Lloret Luna, P., additional, Macias Gallego, A., additional, Castano, S. A. R. A., additional, Garcia, M., additional, Pujol Salvador, C., additional, Barba, J., additional, Redondo, P., additional, Tomasoni, L., additional, Sitia, S., additional, Atzeni, F., additional, Gianturco, L., additional, Ricci, C., additional, Sarzi-Puttini, P., additional, Turiel, M., additional, De Gennaro Colonna, V., additional, Uejima, T., additional, Jaroch, J., additional, Polombo, C., additional, Hughes, A., additional, Vinereanu, D., additional, Evanvelista, A., additional, Leftheriotis, G., additional, Fraser, A. G., additional, Lewczuk, A., additional, Sobkowicz, B., additional, Tomaszuk-Kazberuk, A., additional, Sawicki, R., additional, Hirnle, T., additional, Michalski, B. W., additional, Filipiak, D., additional, Kasprzak, J. D., additional, Lipiec, P., additional, Dalen, H., additional, Mjolstad, O. C., additional, Klykken, B. E., additional, Graven, T., additional, Martensson, M., additional, Olsson, M., additional, Brodin, L.-A., additional, Enache, R., additional, Leiballi, E., additional, Penhall, A., additional, Perry, R., additional, Altman, M., additional, Sinhal, A., additional, Bennetts, J., additional, Chew, D. P., additional, Joseph, M. X., additional, Larsen, L. H., additional, Kristensen, T., additional, Kober, L. V., additional, Kofoed, K. F., additional, Moscoso Costa, F., additional, Ribeiras, R., additional, Brito, J., additional, Boshoff, S., additional, Neves, J., additional, Teles, R., additional, Canada, M., additional, Andrade, M. J., additional, Gouveia, R., additional, Silva, A., additional, Miskovic, A., additional, Poerner, T. P., additional, Stiller, C. S., additional, Goebel, B. G., additional, Moritz, A. M., additional, Stefani, L., additional, Galanti, G. G., additional, Moraldo, M., additional, Bergamini, C., additional, Pabari, P. A., additional, Dhutia, N. M., additional, Malaweera, A. S. N., additional, Willson, K., additional, Davies, J., additional, Hughes, A. D., additional, Xu, X. Y., additional, Francis, D. P., additional, Jasaityte, R., additional, Amundsen, B., additional, Barbosa, D., additional, Loeckx, D., additional, Kiss, G., additional, Orderud, F., additional, Robesyn, V., additional, Claus, P., additional, D'hooge, J., additional, Nao, T., additional, Miura, T., additional, Shams, K., additional, Samir, S., additional, Samir, R., additional, El-Sayed, M., additional, Anwar, A. M., additional, Nosir, Y., additional, Galal, A., additional, Chamsi-Pasha, H., additional, Ciobanu, A., additional, Dulgheru, R., additional, Bennett, S., additional, De Luca, A., additional, Toncelli, L., additional, Cappelli, F., additional, Cappelli, B., additional, Vono, M. C. R., additional, Galanti, G., additional, Zorman, Y., additional, Yilmazer, M. S., additional, Akyildiz, M., additional, Gurol, T., additional, Aydin, A., additional, Dagdeviren, B., additional, and Kalangos, A., additional

Published
2010
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21. Poster Session 1: Ablation of atrial fibrillation

Author

Marti Almor, J., primary, Bazan, V., additional, Matiello, M., additional, Cian, D., additional, Oliva, X., additional, Altaba, C., additional, Guijo, M. A., additional, Bruguera, J., additional, Fiala, M., additional, Sknouril, M., additional, Dorda, M., additional, Chovancik, J., additional, Nevralova, R., additional, Jiravsky, O., additional, Jiravska-Godula, B., additional, Branny, M., additional, Elvan, A., additional, Beukema, W. P., additional, Smit, J. J. J., additional, Delnoy, P. P. H. M., additional, Ramdat Misier, A. R., additional, Tuan, J., additional, Chung, I., additional, Jeilan, M., additional, Kundu, S., additional, Osman, F., additional, Stafford, P., additional, Ng, G. A., additional, Vergara, P., additional, Mazzone, P., additional, Paglino, G., additional, Saviano, M., additional, Crisa, S., additional, Maida, G., additional, Vicedomini, G., additional, Pappone, C., additional, Miyazaki, S., additional, Wright, M., additional, Hocini, M., additional, Jais, P., additional, Haissaguerre, M., additional, Yoshitani, K., additional, Kaitani, K., additional, Hanazawa, K., additional, Nakagawa, Y., additional, Yokokawa, M., additional, Tada, H., additional, Naito, S., additional, Oshima, S., additional, Taniguchi, K., additional, Romanov, A., additional, Pokushalov, E., additional, Shugaev, P., additional, Artemenko, S., additional, Turov, A., additional, Gindele, F. M., additional, Wiedemann, M., additional, Ewertsen, C., additional, Heiderfazel, S., additional, Andresen, D., additional, Miyake, M., additional, Motooka, M., additional, Izumi, T., additional, Izumi, C., additional, Sunthorn, H., additional, Burri, H. B., additional, Gentil, P. G., additional, Shah, D. S., additional, Sugiura, S., additional, Fujii, E., additional, Senga, M., additional, Yamazato, S., additional, Nakamura, M., additional, Ito, M., additional, Den Uijl, D. W., additional, Delgado, V., additional, Tops, L. F., additional, Trines, S. A. I. P., additional, Zeppenfeld, K., additional, Van Der Wall, E. E., additional, Schalij, M. J., additional, Bax, J. J., additional, Pappalardo, A., additional, Forleo, G. B., additional, Avella, A., additional, Bencardino, G., additional, De Girolamo, P. G., additional, Dello Russo, A., additional, Laurenzi, F., additional, Tondo, C., additional, Mueller, H., additional, Burri, H., additional, Gentil-Baron, P., additional, Lerch, R., additional, Shah, D., additional, Shirokova, N., additional, Pedrote Martinez, A. A., additional, Arana, E., additional, Garcia-Riesco, L., additional, Urbano-Moral, J. A., additional, Frutos-Lopez, M., additional, Sanchez-Brotons, J. A., additional, Torres-Llergo, J., additional, Martinez-Martinez, A., additional, Matsuda, H., additional, Harada, T., additional, Nakano, E., additional, Takai, M., additional, Fujita, S., additional, Sasaki, T., additional, Mizuno, K., additional, Miyake, F., additional, Doshi, A., additional, Hummel, J., additional, Daoud, E., additional, Augostini, R., additional, Weiss, R., additional, Hart, D., additional, Houmsse, M., additional, Kalbfleisch, S., additional, Gorzolka, J., additional, Bulkova, V., additional, Wojnarova, D., additional, Neuwirth, R., additional, Januska, J., additional, Cerrato, E., additional, Amellone, C., additional, Tizzani, E., additional, Antolini, M., additional, Massa, R., additional, Golzio, P. G., additional, Comoglio, C., additional, Rinaldi, M., additional, El-Domiaty, H. A., additional, Kamal, H. M., additional, Moubarak, A. M., additional, Mansy, M. M., additional, El-Kerdawy, H., additional, Ahmed, S., additional, Klinkenberg, T. J., additional, Ten Hagen, A., additional, Wiesfeld, A. C. P., additional, Tan, E. S., additional, and Van Gelder, I. C., additional

Published
2009
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24. Pulmonary vein isolation using segmental versus electroanatomical circumferential ablation for paroxysmal atrial fibrillation: over 3-year results of a prospective randomized study.

Author

Fiala M, Chovancík J, Nevralová R, Neuwirth R, Jiravsky O, Nykl I, Sknouril L, Dorda M, Januska J, Branny M, Fiala, Martin, Chovancík, Jan, Nevralová, Renáta, Neuwirth, Radek, Jiravský, Otakar, Nykl, Igor, Sknouril, Libor, Dorda, Miloslav, Januska, Jaroslav, and Branny, Marian

Abstract

Purpose: We tested the hypothesis that electroanatomic pulmonary vein (PV) antra encircling for the PV isolation will improve the outcome in treatment of paroxysmal atrial fibrillation (PAF), compared with segmental PV isolation.Methods: Fifty-four patients underwent segmental PV isolation (group 1) and 56 patients circumferential PV isolation (group 2) for symptomatic PAF in a randomized study.Results: Following single ablation procedure, at the 48 +/- 8 month follow-up, 30 (56%) and 32 (57%) patients in groups 1 and 2 remained free of arrhythmia (P = 0.41). After repeat ablation, 43 (80%) and 45 (80%) patients in groups 1 and 2 were free of arrhythmia without antiarrhythmic drugs (AADs); 48 (89%) and 51 (91%) patients in groups 1 and 2 did not have arrhythmia recurrences without or with AADs.Conclusion: This study demonstrates no advantage in long-term arrhythmia-free clinical outcome after circumferential PV isolation in patients with frequent PAF. [ABSTRACT FROM AUTHOR]

Published
2008
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28. The Cystic Fibrosis Upper and Lower Airway Metagenome.

Author

Pienkowska K, Pust MM, Gessner M, Gaedcke S, Thavarasa A, Rosenboom I, Morán Losada P, Minso R, Arnold C, Hedtfeld S, Dorda M, Wiehlmann L, Mainz JG, Klockgether J, and Tümmler B

Abstract

The microbial metagenome in cystic fibrosis (CF) airways was investigated by whole-genome shotgun sequencing of total DNA isolated from nasal lavage samples, oropharyngeal swabs, and induced sputum samples collected from 65 individuals with CF aged 7 to 50 years. Each patient harbored a personalized microbial metagenome unique in microbial load and composition, the exception being monocultures of the most common CF pathogens Staphylococcus aureus and Pseudomonas aeruginosa from patients with advanced lung disease. The sampling of the upper airways by nasal lavage uncovered the fungus Malassezia restricta and the bacterium Staphylococcus epidermidis as prominent species. Healthy and CF donors harbored qualitatively and quantitatively different spectra of commensal bacteria in their sputa, even in the absence of any typical CF pathogen. If P. aeruginosa, S. aureus, or Stenotrophomonas maltophilia belonged to the trio of the most abundant species in the CF sputum metagenome, common inhabitants of the respiratory tract of healthy subjects, i.e., Eubacterium sulci, Fusobacterium periodonticum, and Neisseria subflava, were present only in low numbers or not detectable. Random forest analysis identified the numerical ecological parameters of the bacterial community, such as Shannon and Simpson diversity, as the key parameters that globally distinguish sputum samples from CF and healthy donors. IMPORTANCE Cystic fibrosis (CF) is the most common life-limiting monogenetic disease in European populations and is caused by mutations in the CFTR gene. Chronic airway infections with opportunistic pathogens are the major morbidity that determines prognosis and quality of life in most people with CF. We examined the composition of the microbial communities of the oral cavity and upper and lower airways in CF patients across all age groups. From early on, the spectrum of commensals is different in health and CF. Later on, when the common CF pathogens take up residence in the lungs, we observed differential modes of depletion of the commensal microbiota in the presence of S. aureus, P. aeruginosa, S. maltophilia, or combinations thereof. It remains to be seen whether the implementation of lifelong CFTR (cystic fibrosis transmembrane conductance regulator) modulation will change the temporal evolution of the CF airway metagenome.

Published
2023
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29. Competitive fitness of Pseudomonas aeruginosa isolates in human and murine precision-cut lung slices.

Author

Cramer N, Nawrot ML, Wege L, Dorda M, Sommer C, Danov O, Wronski S, Braun A, Jonigk D, Fischer S, Munder A, and Tümmler B

Subjects
Animals, Humans, Lung microbiology, Mice, Cystic Fibrosis complications, Cystic Fibrosis microbiology, Pseudomonas Infections microbiology, Pseudomonas aeruginosa genetics
Abstract

Chronic respiratory infections with the gram-negative bacterium Pseudomonas aeruginosa are an important co-morbidity for the quality of life and prognosis of people with cystic fibrosis (CF). Such long-term colonization, sometimes lasting up to several decades, represents a unique opportunity to investigate pathogen adaptation processes to the host. Our studies aimed to resolve if and to what extent the bacterial adaptation to the CF airways influences the fitness of the pathogen to grow and to persist in the lungs. Marker-free competitive fitness experiments of serial P. aeruginosa isolates differentiated by strain-specific SNPs, were performed with murine and human precision cut lung slices (PCLS). Serial P. aeruginosa isolates were selected from six mild and six severe CF patient courses, respectively. MPCLS or hPCLS were inoculated with a mixture of equal numbers of the serial isolates of one course. The temporal change of the composition of the bacterial community during competitive growth was quantified by multi-marker amplicon sequencing. Both ex vivo models displayed a strong separation of fitness traits between mild and severe courses. Whereas the earlier isolates dominated the competition in the severe courses, intermediate and late isolates commonly won the competition in the mild courses. The status of the CF lung disease rather than the bacterial genotype drives the adaptation of P. aeruginosa during chronic CF lung infection. This implies that the disease status of the lung habitat governed the adaptation of P. aeruginosa more strongly than the underlying bacterial clone-type and its genetic repertoire., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Cramer, Nawrot, Wege, Dorda, Sommer, Danov, Wronski, Braun, Jonigk, Fischer, Munder and Tümmler.)

Published
2022
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30. Sequence diversity of the Pseudomonas aeruginosa population in loci that undergo microevolution in cystic fibrosis airways.

Author

Fischer S, Klockgether J, Gonzalez Sorribes M, Dorda M, Wiehlmann L, and Tümmler B

Abstract

Five hundred and thirty-four unrelated Pseudomonas aeruginosa isolates from inanimate habitats, patients with cystic fibrosis (CF) and other human infections were sequenced in 19 genes that had been identified previously as the hot spots of genomic within-host evolution in serial isolates from 12 CF lungs. Amplicon sequencing confirmed a significantly higher sequence diversity of the 19 loci in P. aeruginosa isolates from CF patients compared to those from other habitats, but this overrepresentation was mainly due to the larger share of synonymous substitutions. Correspondingly, non-synonymous substitutions were either rare ( gltT , lepA , ptsP ) or benign ( nuoL , fleR , pelF ) in some loci. Other loci, however, showed an accumulation of non-neutral coding variants. Strains from the CF habitat were often mutated at evolutionarily conserved positions in the elements of stringent response (RelA, SpoT), LPS (PagL), polyamine transport (SpuE, SpuF) and alginate biosynthesis (AlgG, AlgU). The strongest skew towards the CF lung habitat was seen for amino acid sequence variants in AlgG that clustered in the carbohydrate-binding/sugar hydrolysis domain. The master regulators of quorum sensing lasR and rhlR were frequent targets for coding variants in isolates from chronic and acute human infections. Unique variants in lasR showed strong evidence of positive selection indicated by d N / d S values of ~4. The pelA gene that encodes a multidomain enzyme involved in both the formation and dispersion of Pel biofilms carried the highest number of single-nucleotide variants among the 19 genes and was the only gene with a higher frequency of missense mutations in P. aeruginosa strains from non-CF habitats than in isolates from CF airways. PelA protein variants are widely distributed in the P. aeruginosa population. In conclusion, coding variants in a subset of the examined loci are indeed characteristic for the adaptation of P. aeruginosa to the CF airways, but for other loci the elevated mutation rate is more indicative of infections in human habitats ( lasR, rhlR ) or global diversifying selection ( pelA )., Competing Interests: The authors declare that there are no conflicts of interest., (© 2021 The Authors.)

Published
2021
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31. iPSC culture expansion selects against putatively actionable mutations in the mitochondrial genome.

Author

Kosanke M, Davenport C, Szepes M, Wiehlmann L, Kohrn T, Dorda M, Gruber J, Menge K, Sievert M, Melchert A, Gruh I, Göhring G, and Martin U

Subjects
Cell Culture Techniques, Genome, Mitochondrial, Genomic Instability, Humans, Cell Differentiation, Cellular Reprogramming, DNA, Mitochondrial genetics, Induced Pluripotent Stem Cells physiology, Mitochondria genetics, Mutation, Selection, Genetic
Abstract

Therapeutic application of induced pluripotent stem cell (iPSC) derivatives requires comprehensive assessment of the integrity of their nuclear and mitochondrial DNA (mtDNA) to exclude oncogenic potential and functional deficits. It is unknown, to which extent mtDNA variants originate from their parental cells or from de novo mutagenesis, and whether dynamics in heteroplasmy levels are caused by inter- and intracellular selection or genetic drift. Sequencing of mtDNA of 26 iPSC clones did not reveal evidence for de novo mutagenesis, or for any selection processes during reprogramming or differentiation. Culture expansion, however, selected against putatively actionable mtDNA mutations. Altogether, our findings point toward a scenario in which intracellular selection of mtDNA variants during culture expansion shapes the mutational landscape of the mitochondrial genome. Our results suggest that intercellular selection and genetic drift exert minor impact and that the bottleneck effect in context of the mtDNA genetic pool might have been overestimated., (Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.)

Published
2021
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32. Reprogramming enriches for somatic cell clones with small-scale mutations in cancer-associated genes.

Author

Kosanke M, Osetek K, Haase A, Wiehlmann L, Davenport C, Schwarzer A, Adams F, Kleppa MJ, Schambach A, Merkert S, Wunderlich S, Menke S, Dorda M, and Martin U

Subjects
Aged, Cell Cycle, Cell Death, Cell Differentiation, Cell Line, Cells, Cultured, Cellular Reprogramming, Clone Cells chemistry, Human Umbilical Vein Endothelial Cells, Humans, Induced Pluripotent Stem Cells chemistry, Neoplasms pathology, Clone Cells cytology, Induced Pluripotent Stem Cells cytology, Mutation, Neoplasms genetics, Exome Sequencing methods
Abstract

Cellular therapies based on induced pluripotent stem cells (iPSCs) come out of age and an increasing number of clinical trials applying iPSC-based transplants are ongoing or in preparation. Recent studies, however, demonstrated a high number of small-scale mutations in iPSCs. Although the mutational load in iPSCs seems to be largely derived from their parental cells, it is still unknown whether reprogramming may enrich for individual mutations that could lead to loss of functionality and tumor formation from iPSC derivatives. 30 hiPSC lines were analyzed by whole exome sequencing. High accuracy amplicon sequencing showed that all analyzed small-scale variants pre-existed in their parental cells and that individual mutations present in small subpopulations of parental cells become enriched among hiPSC clones during reprogramming. Among those, putatively actionable driver mutations affect genes related to cell-cycle control, cell death, and pluripotency and may confer a selective advantage during reprogramming. Finally, a short hairpin RNA (shRNA)-based experimental approach was applied to provide additional evidence for the individual impact of such genes on the reprogramming efficiency. In conclusion, we show that enriched mutations in curated onco- and tumor suppressor genes may account for an increased tumor risk and impact the clinical value of patient-derived hiPSCs., Competing Interests: Declaration of interests The authors declare no competing interests., (Copyright © 2021 The American Society of Gene and Cell Therapy. Published by Elsevier Inc. All rights reserved.)

Published
2021
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33. Genome-Wide Methylation Mapping Using Nanopore Sequencing Technology Identifies Novel Tumor Suppressor Genes in Hepatocellular Carcinoma.

Author

Davenport CF, Scheithauer T, Dunst A, Bahr FS, Dorda M, Wiehlmann L, and Tran DDH

Subjects
Genome-Wide Association Study, Hep G2 Cells, Humans, Carcinoma, Hepatocellular genetics, Carcinoma, Hepatocellular metabolism, DNA Methylation, DNA, Neoplasm genetics, DNA, Neoplasm metabolism, Genes, Tumor Suppressor, Liver Neoplasms genetics, Liver Neoplasms metabolism, Nanopore Sequencing
Abstract

Downregulation of multiple tumor suppressor genes (TSGs) plays an important role in cancer formation. Recent evidence has accumulated that cancer progression involves genome-wide alteration of epigenetic modifications, which may cause downregulation of the tumor suppressor gene. Using hepatocellular carcinoma (HCC) as a system, we mapped 5-methylcytosine signal at a genome-wide scale using nanopore sequencing technology to identify novel TSGs. Integration of methylation data with gene transcription profile of regenerated liver and primary HCCs allowed us to identify 10 potential tumor suppressor gene candidates. Subsequent validation led us to focus on functionally characterizing one candidate-glucokinase (GCK). We show here that overexpression of GCK inhibits the proliferation of HCC cells via induction of intracellular lactate accumulation and subsequently causes energy crisis due to NAD+ depletion. This suggests GCK functions as a tumor suppressor gene and may be involved in HCC development. In conclusion, these data provide valuable clues for further investigations of the process of tumorigenesis in human cancer.

Published
2021
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34. Ultrasound diagnosis of a case of transient bilateral vocal cord paralysis secondary to local anesthetic infiltration.

Author

Muñoz C, de Miguel M, Rochera MI, Dorda MD, Caubet E, and González O

Subjects
Anesthetics, Local adverse effects, Humans, Ultrasonography, Vocal Cords diagnostic imaging, Recurrent Laryngeal Nerve Injuries, Vocal Cord Paralysis chemically induced
Abstract

One of the most important complications associated with thyroid and parathyroid surgery is vocal cord paralysis due to a recurrent laryngeal nerve injury. Recurrent laryngeal nerve injury paralysis induced by local anesthetics is a rare complication with very few published cases Various techniques are available for diagnosing vocal cord paralysis, including, flexible fiberoptic laryngoscopy, videostrobolaryngoscopy and indirect laryngoscopy. However, these techniques are expensive and are often associated with pain and discomfort among patients. Considering these disadvantages, transcutaneous laryngeal ultrasound is an alternative imaging tool for vocal cord examination in patients undergoing thyroid and parathyroid surgery. We describe a case which was sonographically diagnosed a transient bilateral vocal cord paralysis after the local infiltration of 10mL of 2% mepivacaine administered for the revision of the surgical wound due to a subcutaneous hematoma that occurred after a subtotal parathyroidectomy., (Copyright © 2020 Sociedad Española de Anestesiología, Reanimación y Terapéutica del Dolor. Publicado por Elsevier España, S.L.U. All rights reserved.)

Published
2021
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35. Novel candidate genes for ECT response prediction-a pilot study analyzing the DNA methylome of depressed patients receiving electroconvulsive therapy.

Author

Moschny N, Zindler T, Jahn K, Dorda M, Davenport CF, Wiehlmann L, Maier HB, Eberle F, Bleich S, Neyazi A, and Frieling H

Subjects
Adult, Aged, Female, Humans, Longitudinal Studies, Male, Middle Aged, Pilot Projects, Treatment Outcome, Young Adult, DNA Methylation, Depressive Disorder, Major therapy, Electroconvulsive Therapy methods
Abstract

Background: Major depressive disorder (MDD) represents a serious global health concern. The urge for efficient MDD treatment strategies is presently hindered by the incomplete knowledge of its underlying pathomechanism. Despite recent progress (highlighting both genetics and the environment, and thus DNA methylation, to be relevant for its development), 30-50% of MDD patients still fail to reach remission with standard treatment approaches. Electroconvulsive therapy (ECT) is one of the most powerful options for the treatment of pharmacoresistant depression; nevertheless, ECT remission rates barely reach 50% in large-scale naturalistic population-based studies. To optimize MDD treatment strategies and enable personalized medicine in the long- term, prospective indicators of ECT response are thus in great need. Because recent target-driven analyses revealed DNA methylation baseline differences between ECT responder groups, we analyzed the DNA methylome of depressed ECT patients using next-generation sequencing. In this pilot study, we did not only aim to find novel targets for ECT response prediction but also to get a deeper insight into its possible mechanism of action., Results: Longitudinal DNA methylation analysis of peripheral blood mononuclear cells isolated from a cohort of treatment-resistant MDD patients (n = 12; time points: before and after 1st and last ECT, respectively) using a TruSeq-Methyl Capture EPIC Kit for library preparation, led to the following results: (1) The global DNA methylation differed neither between the four measured time points nor between ECT responders (n = 8) and non-responders (n = 4). (2) Analyzing the DNA methylation variance for every probe (=1476812 single CpG sites) revealed eight novel candidate genes to be implicated in ECT response (protein-coding genes: RNF175, RNF213, TBC1D14, TMC5, WSCD1; genes encoding for putative long non-coding RNA transcripts: AC018685.2, AC098617.1, CLCN3P1). (3) In addition, DNA methylation of two CpG sites (located within AQP10 and TRERF1) was found to change during the treatment course., Conclusions: We suggest ten novel candidate genes to be implicated in either ECT response or its possible mechanism. Because of the small sample size of our pilot study, our findings must be regarded as preliminary.

Published
2020
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36. Intraclonal competitive fitness of longitudinal cystic fibrosis Pseudomonas aeruginosa airway isolates in liquid cultures.

Author

Cramer N, Fischer S, Hedtfeld S, Dorda M, and Tümmler B

Subjects
Adaptation, Physiological genetics, Base Sequence, Evolution, Molecular, Genomics, Humans, Phenotype, Pseudomonas Infections, Pseudomonas aeruginosa genetics, Pseudomonas aeruginosa isolation & purification, Sequence Analysis, DNA, Adaptation, Physiological physiology, Cystic Fibrosis microbiology, Lung microbiology, Pseudomonas aeruginosa growth & development, Pseudomonas aeruginosa metabolism
Abstract

The metabolically versatile Pseudomonas aeruginosa inhabits biotic and abiotic environments including the niche of cystic fibrosis (CF) airways. This study investigated how the adaptation to CF lungs affects the within-clone fitness of P. aeruginosa to grow and persist in liquid cultures in the presence of the clonal ancestors. Longitudinal clonal P. aeruginosa isolates that had been collected from 12 CF donors since the onset of colonization for up to 30 years was subjected to within-clone competition experiments. The relative quantities of individual strains were determined by marker-free amplicon sequencing of multiplex PCR products of strain-specific nucleotide sequence variants, a novel method that is generally applicable to studies in evolutionary genetics and microbial ecology with real-world strain collections. For 10 of the 12 examined patient courses, P. aeruginosa isolates of the first years of colonization grew faster in the presence of their clonal progeny than alone. Single growth of individual strains showed no temporal trend with colonization time, but in co-culture, the early isolates out-competed their clonal progeny. Irrespective of the genetic make-up of the clone and its genomic microevolution in CF lungs, the early isolates expressed fitness traits to win the within-clone competition that were absent in their progeny., (© 2020 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd.)

Published
2020
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37. Effects of Lumacaftor-Ivacaftor Therapy on Cystic Fibrosis Transmembrane Conductance Regulator Function in Phe508del Homozygous Patients with Cystic Fibrosis.

Author

Graeber SY, Dopfer C, Naehrlich L, Gyulumyan L, Scheuermann H, Hirtz S, Wege S, Mairbäurl H, Dorda M, Hyde R, Bagheri-Hanson A, Rueckes-Nilges C, Fischer S, Mall MA, and Tümmler B

Subjects
Adolescent, Adult, Child, Drug Combinations, Female, Germany, Homozygote, Humans, Male, Prospective Studies, Young Adult, Aminophenols therapeutic use, Aminopyridines therapeutic use, Benzodioxoles therapeutic use, Biomarkers blood, Chloride Channel Agonists therapeutic use, Cystic Fibrosis drug therapy, Cystic Fibrosis genetics, Cystic Fibrosis Transmembrane Conductance Regulator drug effects, Quinolones therapeutic use
Abstract

Rationale: The combination of the CFTR (cystic fibrosis transmembrane conductance regulator) corrector lumacaftor with the potentiator ivacaftor has been approved for the treatment of patients with cystic fibrosis homozygous for the Phe508del CFTR mutation. The phase 3 trials examined clinical outcomes but did not evaluate CFTR function in patients., Objectives: To examine the effect of lumacaftor-ivacaftor on biomarkers of CFTR function in Phe508del homozygous patients with cystic fibrosis aged 12 years and older., Methods: This prospective observational study assessed clinical outcomes including FEV 1 % predicted and body mass index, and CFTR biomarkers including sweat chloride concentration, nasal potential difference, and intestinal current measurement before and 8-16 weeks after initiation of lumacaftor-ivacaftor., Measurements and Main Results: A total of 53 patients were enrolled in the study, and 52 patients had baseline and follow-up measurements. After initiation of lumacaftor-ivacaftor sweat chloride concentrations were reduced by 17.8 mmol/L (interquartile range [IQR], -25.9 to -6.1; P < 0.001), nasal potential difference showed partial rescue of CFTR function in nasal epithelia to a level of 10.2% (IQR, 0.0-26.1; P < 0.011), and intestinal current measurement showed functional improvement in rectal epithelia to a level of 17.7% of normal (IQR, 10.8-29.0; P < 0.001). All patients improved in at least one CFTR biomarker, but no correlations were found between CFTR biomarker responses and clinical outcomes., Conclusions: Lumacaftor-ivacaftor results in partial rescue of Phe508del CFTR function to levels comparable to the lower range of CFTR activity found in patients with residual function mutations. Functional improvement was detected even in the absence of short-term improvement of FEV 1 % predicted and body mass index. Clinical trial registered with www.clinicaltrials.gov (NCT02807415).

Published
2018
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38. Impact of sample processing on human airways microbial metagenomes.

Author

Wiehlmann L, Pienkowska K, Hedtfeld S, Dorda M, and Tümmler B

Subjects
Artifacts, DNA chemistry, High-Throughput Nucleotide Sequencing methods, Reproducibility of Results, Sample Size, Sensitivity and Specificity, Bacterial Typing Techniques methods, DNA genetics, Lung microbiology, Metagenome genetics, Microbial Consortia genetics, Sequence Analysis, DNA methods, Specimen Handling methods
Abstract

Whole metagenome shotgun sequencing provides information about the gene content and the composition of microbial communities provided that the processing of the samples does not introduce a methodology-driven bias. We tested the impact of DNA isolation and storage period on the metagenome profile. Deep throat swabs were collected from healthy adults and an infected infant. DNA was isolated by sonification or enzymatic lysis either immediately or after 24h storage in agar gel Amies transport medium at room temperature. Disruption of cells and subsequent fragmentation of DNA by sonification was as suitable as the common enzymatic lysis to generate high-quality metagenomes particularly for low total DNA input of less than ten nanograms. Conversely, storage of samples for 24h produced severely distorted metagenomes. The majority of species became less abundant or even extinct, whereas a few Streptococcus, Neisseria and Haemophilus spp. proliferated so that the total number of bacterial reads increased at the expense of human reads. We recommend that samples for metagenome analysis should be immediately processed or frozen at -80°C., (Copyright © 2017 Elsevier B.V. All rights reserved.)

Published
2017
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39. Multilocus amplicon sequencing of Pseudomonas aeruginosa cystic fibrosis airways isolates collected prior to and after early antipseudomonal chemotherapy.

Author

Fischer S, Greipel L, Klockgether J, Dorda M, Wiehlmann L, Cramer N, and Tümmler B

Subjects
Bacterial Typing Techniques methods, Child, Chronic Disease, Drug Monitoring methods, Female, Germany epidemiology, Humans, Infant, Male, Multilocus Sequence Typing methods, Time-to-Treatment, Young Adult, Anti-Bacterial Agents therapeutic use, Bacterial Proteins classification, Bacterial Proteins genetics, Cystic Fibrosis complications, Cystic Fibrosis diagnosis, Cystic Fibrosis epidemiology, Cystic Fibrosis microbiology, Pseudomonas Infections diagnosis, Pseudomonas Infections etiology, Pseudomonas Infections physiopathology, Pseudomonas aeruginosa drug effects, Pseudomonas aeruginosa isolation & purification, Pseudomonas aeruginosa physiology
Abstract

Background: Early antimicrobial chemotherapy can prevent or at least delay chronic cystic fibrosis (CF) airways infections with Pseudomonas aeruginosa., Methods: During a 10-year study period P. aeruginosa was detected for the first time in 54 CF patients regularly seen at the CF centre Hannover. Amplicon sequencing of 34 loci of the P. aeruginosa core genome was performed in baseline and post-treatment isolates of the 15 CF patients who had remained P. aeruginosa - positive after the first round of antipseudomonal chemotherapy., Results: Deep sequencing uncovered coexisting alternative nucleotides at in total 33 of 55,284 examined genome positions including six non-synonymous polymorphisms in the lasR gene, a key regulator of quorum sensing. After early treatment 42 of 50 novel nucleotide substitutions had emerged in exopolysaccharide biosynthesis, efflux pump and porin genes., Conclusions: Early treatment selects pathoadaptive mutations in P. aeruginosa that are typical for chronic infections of CF lungs., (Copyright © 2016 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.)

Published
2017
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40. Molecular Epidemiology of Mutations in Antimicrobial Resistance Loci of Pseudomonas aeruginosa Isolates from Airways of Cystic Fibrosis Patients.

Author

Greipel L, Fischer S, Klockgether J, Dorda M, Mielke S, Wiehlmann L, Cramer N, and Tümmler B

Subjects
Amino Acid Sequence, Amino Acid Substitution, Anti-Bacterial Agents pharmacology, Bacterial Proteins chemistry, Bacterial Proteins genetics, Bacterial Proteins metabolism, Clone Cells, Cystic Fibrosis drug therapy, Cystic Fibrosis microbiology, Cystic Fibrosis pathology, High-Throughput Nucleotide Sequencing, Humans, Microbial Sensitivity Tests, Molecular Epidemiology, Protein Conformation, alpha-Helical, Protein Interaction Domains and Motifs, Pseudomonas Infections drug therapy, Pseudomonas Infections microbiology, Pseudomonas Infections pathology, Pseudomonas aeruginosa drug effects, Pseudomonas aeruginosa isolation & purification, Pseudomonas aeruginosa metabolism, Respiratory System drug effects, Respiratory System microbiology, Respiratory System pathology, Sequence Alignment, Drug Resistance, Multiple, Bacterial genetics, Genes, Bacterial, Genetic Loci, Genome, Bacterial, Mutation, Pseudomonas aeruginosa genetics
Abstract

The chronic airway infections with Pseudomonas aeruginosa in people with cystic fibrosis (CF) are treated with aerosolized antibiotics, oral fluoroquinolones, and/or intravenous combination therapy with aminoglycosides and β-lactam antibiotics. An international strain collection of 361 P. aeruginosa isolates from 258 CF patients seen at 30 CF clinics was examined for mutations in 17 antimicrobial susceptibility and resistance loci that had been identified as hot spots of mutation by genome sequencing of serial isolates from a single CF clinic. Combinatorial amplicon sequencing of pooled PCR products identified 1,112 sequence variants that were not present in the genomes of representative strains of the 20 most common clones of the global P. aeruginosa population. A high frequency of singular coding variants was seen in spuE, mexA, gyrA, rpoB, fusA1, mexZ, mexY, oprD, ampD, parR, parS, and envZ (amgS), reflecting the pressure upon P. aeruginosa in lungs of CF patients to generate novel protein variants. The proportion of nonneutral amino acid exchanges was high. Of the 17 loci, mexA, mexZ, and pagL were most frequently affected by independent stop mutations. Private and de novo mutations seem to play a pivotal role in the response of P. aeruginosa populations to the antimicrobial load and the individual CF host., (Copyright © 2016, American Society for Microbiology. All Rights Reserved.)

Published
2016
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41. The cystic fibrosis lower airways microbial metagenome.

Author

Moran Losada P, Chouvarine P, Dorda M, Hedtfeld S, Mielke S, Schulz A, Wiehlmann L, and Tümmler B

Abstract

Chronic airway infections determine most morbidity in people with cystic fibrosis (CF). Herein, we present unbiased quantitative data about the frequency and abundance of DNA viruses, archaea, bacteria, moulds and fungi in CF lower airways. Induced sputa were collected on several occasions from children, adolescents and adults with CF. Deep sputum metagenome sequencing identified, on average, approximately 10 DNA viruses or fungi and several hundred bacterial taxa. The metagenome of a CF patient was typically found to be made up of an individual signature of multiple, lowly abundant species superimposed by few disease-associated pathogens, such as Pseudomonas aeruginosa and Staphylococcus aureus , as major components. The host-associated signatures ranged from inconspicuous polymicrobial communities in healthy subjects to low-complexity microbiomes dominated by the typical CF pathogens in patients with advanced lung disease. The DNA virus community in CF lungs mainly consisted of phages and occasionally of human pathogens, such as adeno- and herpesviruses. The S. aureus and P. aeruginosa populations were composed of one major and numerous minor clone types. The rare clones constitute a low copy genetic resource that could rapidly expand as a response to habitat alterations, such as antimicrobial chemotherapy or invasion of novel microbes., Competing Interests: can be found alongside this article at openres.ersjournals.com

Published
2016
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42. Intraclonal genome diversity of the major Pseudomonas aeruginosa clones C and PA14.

Author

Fischer S, Klockgether J, Morán Losada P, Chouvarine P, Cramer N, Davenport CF, Dethlefsen S, Dorda M, Goesmann A, Hilker R, Mielke S, Schönfelder T, Suerbaum S, Türk O, Woltemate S, Wiehlmann L, and Tümmler B

Subjects
Conserved Sequence, Environmental Microbiology, Gene Transfer, Horizontal, Genome, Bacterial, Humans, Pseudomonas Infections microbiology, Pseudomonas aeruginosa isolation & purification, Genetic Variation, Genotype, Pseudomonas aeruginosa classification, Pseudomonas aeruginosa genetics
Abstract

Bacterial populations differentiate at the subspecies level into clonal complexes. Intraclonal genome diversity was studied in 100 isolates of the two dominant Pseudomonas aeruginosa clones C and PA14 collected from the inanimate environment, acute and chronic infections. The core genome was highly conserved among clone members with a median pairwise within-clone single nucleotide sequence diversity of 8 × 10(-6) for clone C and 2 × 10(-5) for clone PA14. The composition of the accessory genome was, on the other hand, as variable within the clone as between unrelated clones. Each strain carried a large cargo of unique genes. The two dominant worldwide distributed P. aeruginosa clones combine an almost invariant core with the flexible gain and loss of genetic elements that spread by horizontal transfer., (© 2015 The Authors. Environmental Microbiology Reports published by Society for Applied Microbiology and John Wiley & Sons Ltd.)

Published
2016
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43. Multivariate Analysis of Correspondence between Left Atrial Volumes Assessed by Echocardiography and 3-Dimensional Electroanatomic Mapping in Patients with Atrial Fibrillation.

Author

Havranek S, Fiala M, Bulava A, Sknouril L, Dorda M, Bulkova V, Fingrova Z, Souckova L, Palecek T, Simek J, Linhart A, and Wichterle D

Subjects
Female, Humans, Male, Middle Aged, Multivariate Analysis, Organ Size, ROC Curve, Regression Analysis, Atrial Fibrillation diagnostic imaging, Echocardiography, Heart Atria diagnostic imaging, Heart Atria pathology, Imaging, Three-Dimensional
Abstract

Background: Left atrial (LA) enlargement is a predictor of worse outcome after catheter ablation for atrial fibrillation (AF). Widely used two-dimensional (2D)-echocardiography is inaccurate and underestimates real LA volume (LAV). We hypothesized that baseline clinical characteristics of patients can be used to adjust 2D-ECHO indices of LAV in order to minimize this disagreement., Methods: The study enrolled 535 patients (59 ± 9 years; 67% males; 43% paroxysmal AF) who underwent catheter ablation for AF in three specialized centers. We investigated multivariately the relationship between 2D-echocardiographic indices of LA size, specifically LA diameter in M-mode in the parasternal long-axis view (LAD), LAV assessed by the prolate-ellipsoid method (LAVEllipsoid), LAV by the planimetric method (LAVPlanimetry), and LAV derived from 3D-electroanatomic mapping (LAVCARTO)., Results: Cubed LAD of 106 ± 45 ml, LAVEllipsoid of 72 ± 24 ml and LAVPlanimetry of 88 ± 30 ml correlated only modestly (r = 0.60, 0.69, and 0.53, respectively) with LAVCARTO of 137 ± 46 ml, which was significantly underestimated with a bias (±1.96 standard deviation) of -31 (-111; +49) ml, -64 (-132; +2) ml, and -49 (-125; +27) ml, respectively; p < 0.0001 for their mutual difference. LA enlargement itself, age, gender, type of AF, and the presence of structural heart disease were independent confounders of measurement error of 2D-echocardiographic LAV., Conclusion: Accuracy and precision of all 2D-echocardiographic LAV indices are poor. Their agreement with true LAV can be significantly improved by multivariate adjustment to clinical characteristics of patients.

Published
2016
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44. A prospective evaluation of haemodynamics, functional status, and quality of life after radiofrequency catheter ablation of long-standing persistent atrial fibrillation.

Author

Fiala M, Wichterle D, Bulková V, Sknouril L, Nevralová R, Toman O, Dorda M, Januska J, and Spinar J

Subjects
Activities of Daily Living psychology, Adult, Aged, Atrial Fibrillation diagnosis, Catheter Ablation adverse effects, Chronic Disease, Depression etiology, Female, Humans, Longitudinal Studies, Male, Middle Aged, Postoperative Complications etiology, Prospective Studies, Treatment Outcome, Atrial Fibrillation psychology, Atrial Fibrillation surgery, Catheter Ablation psychology, Depression psychology, Postoperative Complications psychology, Quality of Life psychology, Recovery of Function
Abstract

Aims: Clinical benefit from ablation for long-standing persistent atrial fibrillation has remained unknown. We hypothesized that successful ablation of long-standing persistent atrial fibrillation would improve haemodynamics, functional status, and quality of life., Methods and Results: A total of 160 patients (aged 59 ± 9 years, 23% females) undergoing ablation of long-standing (median of 28 months) persistent atrial were enrolled in this prospective study. Morphological and functional echocardiographic parameters, N-terminal prohormone of brain natriuretic peptide (NT-proBNP), maximum oxygen consumption during exercise test (VO2 max), and quality of life were assessed at baseline and 1 year after the ablation.  At the 1-year follow-up visit, 81% patients were examined in sinus rhythm (after repeat ablation in 38% patients). Left atrial appendage outflow velocity increased from 44 ± 20 to 58 ± 23 cm/s, left ventricular ejection fraction from 54 ± 9 to 59 ± 5%, and VO2 max from 20.4 ± 6.4 to 23.7 ± 8.1 mL/kg/min; NT-proBNP decreased from median 897 (interquartile range 603-1424) to 230 (interquartile range 120-420) pg/mL (all P < 0.0001). These beneficial effects of ablation were predominantly associated with the presence of sinus rhythm. Quality of life (range 0-100) increased significantly (EQ-5D index: from 68.8 ± 12.5 to 75.4 ± 14.4; EQ-VAS score: from 62.8 ± 13.2 to 70.6 ± 13.8; both P < 0.0001)., Conclusion: Ablation of long-standing persistent atrial fibrillation was associated with significant recovery of haemodynamics and exercise capacity that projected onto the long-term improvement in quality of life.

Published
2014
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45. [Outcomes of catheter ablation of atrial fibrillation in patients over 65 years of age].

Author

Fiala M, Skňouřil L, Toman O, Pindor J, Wojnarová D, Bulková V, Chovančík J, Dorda M, Szymeczek H, Neuwirth R, Vavřík D, Krawiec S, Jiravský O, Rybka L, Lábrová R, Januška J, and Spinar J

Subjects
Aged, Female, Humans, Male, Treatment Outcome, Atrial Fibrillation surgery, Catheter Ablation
Abstract

Purpose: This study assessed ablation techniques, recurrent arrhythmias, long-term outcomes, and complications of catheter ablation for atrial fibrillation (AF) in patients 65 years of age., Methods: Consecutive patients aged < 65 years (n = 653) vs 65 years (n = 213), who underwent catheter ablation of AF in the course of eight years, were compared. Ablation strategy and procedure endpoints were left at the operators discretion., Results: The group of patients 65 years comprised more females (p < 0.001), and more frequently presented with persistent AF (p = 0.010). These patients less frequently underwent simple pulmonary vein isolation (p = 0.017); on the contrary, extensive ablation including coronary sinus intervention was more common (p = 0.020). There was no difference in repeat ablation procedures (25 % vs 26 % patients; p = 0.823, or 1.4 vs 1.5 ablation procedures/1 patients; p = 0.479, respectively). Spectrum of recurrent arrhythmias did not differ between the groups except for more frequent paroxysmal AF before the first repeat ablation in patients < 65 years (p = 0.050). At the end of 49 ± 26 month total follow-up, stable sinus rhythm (SR) was achieved in 85 % patients < 65 years vs 76 % patients 65 years (p = 0.318). To maintain stable SR, older patients more often continued to take antiarrhythmic medication (p = 0.054). More serious complication occurred in 3.8 % of the patients 65 years vs 2.1 % of the patients < 65 years of age (p = 0.207)., Conclusion: Patients 65 years of age achieved insignificantly worse long-term outcome after insignificantly fewer repeat ablation procedures, and with more frequent use of antiarrhythmic drugs. SR maintenance and risk of complications were, however, favorable.

Published
2013

46. Results of complex left atrial ablation of long-lasting persistent atrial fibrillation.

Author

Fiala M, Chovancík J, Wojnarová D, Bulková V, Szymeczek H, Nevralová R, Neuwirth R, Jiravský O, Sknouril L, Dorda M, Januska J, and Branny M

Subjects
Adult, Aged, Atrial Fibrillation physiopathology, Chi-Square Distribution, Electrophysiologic Techniques, Cardiac, Female, Humans, Male, Middle Aged, Statistics, Nonparametric, Treatment Outcome, Atrial Fibrillation surgery, Catheter Ablation methods
Abstract

Purpose: The aim of the study was to identify variables associated with successful long-term maintenance of sinus rhythm (SR) after a single ablation of long-lasting persistent atrial fibrillation (AF)., Methods: Complex left atrial (LA) ablation was performed in 100 patients. Restoration of SR by ablation was the desired procedure endpoint., Results: SR was restored by ablation in 38 patients during the first procedure. Following one ablation, 50 patients remained in SR for 31 +/- 14 months. SR maintenance was associated with shorter duration of the persistent AF (median 14 vs. 22 months; P = 0.05), lower proportion of the LA points exhibiting voltage <0.2 mV (median 20% vs. 33%; P = 0.006), and higher proportion of LA points showing voltage >1 mV (median 15% vs. 11%; P = 0.02)., Conclusion: Among clinical variables, shorter duration of persistent AF and higher voltage recorded around the LA predicted long-term maintenance of SR after single ablation.

Published
2008
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47. [Recurrent arrhythmias after catheter ablation of originally paroxysmal atrial fibrillation and results of repeat ablation].

Author

Fiala M, Chovancík J, Moravec R, Wojnarová D, Szymeczek H, Neuwirth R, Nevalová R, Jiravský O, Januska J, Sknouril L, Dorda M, Indrák J, Cerný J, Nykl I, and Branny M

Subjects
Adult, Aged, Arrhythmias, Cardiac physiopathology, Arrhythmias, Cardiac surgery, Female, Humans, Male, Middle Aged, Recurrence, Reoperation, Arrhythmias, Cardiac etiology, Atrial Fibrillation surgery, Catheter Ablation methods
Abstract

Aims: The aim is a description of the recurrent arrhythmias after previous ablation of paroxysmal atrial fibrillation (AF), and the results of a repeat catheter ablation., Methods: A repeat ablation was performed in 76 patients (18 females, 54 +/- 11 years) in 96 procedures, which was 21% out of 362 patients, who had undergone the first ablation for a paroxysmal AF. The endpoints of the repeat ablation were re-isolation of the pulmonary veins (PV) and termination of a spontaneous or induced arrhythmia and restoration of a stable sinus rhythm (SR), and possibly achievement of noninducibility of any arrhythmia., Results: Clinical left atrial tachycardia (LAT) was present in 10 (13%) patients before the first, and in 5 (25%) patients before the second repeat ablation. Arrhythmia arising from an arrhythmogenic PV due to the conduction recovery into the left atrium (LA) was found in 50 (66%) patients during the first, and in 7 (35%) patients during the second repeat ablation. Arrhythmias, predominantly of the reentry mechanism and originating in the LA free wall, were found in 26 (34%), respectively 13 (65%) during the first or the second repeat ablation. All arrhythmias from PVs were terminated by a PV encircling ablation. Substrate-related arrhythmias were terminated by ablation except for 2 (3%) patients during the first and 3 (15%) patients during the second repeat ablation. Persistent AF was mainly terminated via conversion into a LAT. In these cases, the ablation sites leading to the SR restoration were, similarly to the primary LATs, located predominantly in the LA anterior wall. During the 22 +/- 13 months follow-up, 68 (89%) patients were free of AF, 54 (71%) patients off the antiarrhythmic drugs and 14 (18%) patients with the class I or III antiarrhythmic drugs., Conclusion: AF associated with PV-LA re-connection dominated prior to the first repeat ablation, then the proportion of the substrate-related arrhythmias from the LA free wall increased. Clinical efficacy of the repeat ablation is high.

Published
2007

48. Atrial macroreentry tachycardia in patients without obvious structural heart disease or previous cardiac surgical or catheter intervention: characterization of arrhythmogenic substrates, reentry circuits, and results of catheter ablation.

Author

Fiala M, Chovancík J, Neuwirth R, Nevralová R, Jiravský O, Sknouril L, Dorda M, Januska J, Vodzinská A, Cerný J, Nykl I, and Branny M

Subjects
Aged, Cardiac Catheterization, Female, Humans, Male, Middle Aged, Tachycardia, Ectopic Atrial complications, Treatment Outcome, Ventricular Dysfunction, Left complications, Ventricular Dysfunction, Left physiopathology, Body Surface Potential Mapping, Catheter Ablation, Heart Conduction System physiopathology, Heart Conduction System surgery, Tachycardia, Ectopic Atrial physiopathology, Tachycardia, Ectopic Atrial surgery
Abstract

Introduction: Atrial macroreentry tachycardia (AMRT) in patients without obvious structural heart disease or previous surgical or catheter intervention has not been characterized in detail., Methods and Results: Electroanatomical mapping and ablation of right or left AMRT were performed in 33 patients. Right atrial central conduction obstacle was formed by an electrically silent area (ESA) in 15 (68%) patients and by a line of double potentials (DPs) in seven (32%) patients. Left atrial ESAs were found in all 11 patients with the left AMRT. Reentry circuit was reconstructed in 19 (86%) patients with right AMRT and seven (64%) patients with left AMRT. Of the ESA-related right AMRT, eight (50%) were double-loop reentry circuits utilizing a narrow critical isthmus within the ESA and eight (50%) were single-loop reentry circuits with a critical isthmus bounded by ESA and either ostium of the vena cava. Single-loop DP-related AMRTs had the critical isthmus between the DP line and the ostium of the inferior vena cava (IVC). Left AMRTs included a variety of single-, double-, or triple-loop reentry circuits and their critical isthmuses. During the 37 +/- 15 month follow-up, atrial tachyarrhythmia-free clinical outcome was achieved in 21 (95%) patients (18 patients, 82%, without antiarrhythmic drugs) with the right AMRT and in nine (82%) patients (six patients, 55%, without antiarrhythmic drugs) with the left AMRT., Conclusion: The majority of right and left AMRTs were related to the presence of ESA. Ablation can be successful with a favorable risk of atrial tachyarrhythmia recurrence.

Published
2007
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49. [Catheter ablation of chronic atrial fibrillation using circumferential and complex linear lesions in the left atrium: modes of arrhythmia termination and long-term clinical outcome].

Author

Fiala M, Chovancík J, Neuwirth R, Nevralová R, Jiravský O, Szymeczek H, Wojnarová D, Moravec R, Sknouril L, Dorda M, Januska J, Nykl I, Cerný J, and Branny M

Subjects
Adult, Aged, Atrial Fibrillation complications, Atrial Fibrillation diagnosis, Electrophysiologic Techniques, Cardiac, Female, Humans, Male, Middle Aged, Atrial Fibrillation surgery, Catheter Ablation methods
Abstract

Objective: The aim of the article is to present the method and results of catheter ablation for chronic atrial fibrillation., Method: Catheter ablation for chronic atrial fibrillation was performed in 82 patients (18 females, aged 54 +/- 10 years), in 112 ablation procedures. Mean duration of the chronic phase of atrial fibrillation was 28 +/- 28 months. Before ablation, amiodarone was administered without effect to 74 (90%) patients, and was counter-indicated in 8 (10%) patients. Ablation strategy consisted of circumferential lesions around the pulmonary veins and of complex linear lesions in the left atrium. Full pulmonary vein antra isolation, and sinus rhythm restoration, or at least converting atrial fibrillation into the left atrial tachycardia, were the procedure end points., Results: Sinus rhythm was restored by ablation at least in one of the ablation procedures in 43 (52%) patients. During the follow-up period spanning 17.3 +/- 11.6 months after the last ablation, stable sinus rhythm was achieved in 63 (77%) patients, of whom 38 (60%) had their sinus rhythm restored by ablation and another 14 (22%) their atrial fibrillation converted into the left atrial tachycardia. Of the 63 patients with stable sinus rhythm, class I or III antiarrhythmic medication has been maintained in 21 (33%) patients, and amiodarone has been taken by 13 (21%) patients., Conclusion: Catheter ablation of chronic atrial fibrillation is potentially highly effective in long-term restoration of sinus rhythm.

Published
2007

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