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5. A novel c.1759T>G variant in follicle-stimulating hormone-receptor gene is concordant with male determination in the flathead grey mullet (Mugil cephalus).

Author

Curzon, Arie Y., Dor, Lior, Shirak, Andrey, Meiri-Ashkenazi, Iris, Rosenfeld, Hana, Ron, Micha, and Seroussi, Eyal

Subjects
*STRIPED mullet, *GRAY mullets, *DNA sequencing, *SHOTGUN sequencing, *HORMONE receptors, *GONADS
Abstract

Various master key regulators (MKRs) that control a binary switch of sex determination (SD) have been found in fish; these provide an excellent model for the study of vertebrate genetic SD. The SD region in flathead grey mullet has been previously mapped to a 1 Mbp region harboring 27 genes, of which one is follicle-stimulating hormone receptor (fshr). Although this gene is involved in gonad differentiation and function, it has not been considered as an MKR of SD. We systematically investigated polymorphism in mullet fshr using DNA shotgun sequences, and compared them between males and females. Capable of encoding nonconservative amino acid substitutions, c.1732G>A and c.1759T>G exhibited association with sex on a population level (N = 83; P 6.7×10-19). Hence, 1732 A and 1759 G represent a male-specific haplotype of the gene, designated as "fshry." Additional flanking SNPs showed a weaker degree of association with sex, delimiting the SD critical region to 143 nucleotides on exon 14. Lack of homozygotes for fshry, and the resulting divergence from Hardy- Weinberg equilibrium (N = 170; P 3.9 ×10-5 ), were compatible with a male heterogametic model (XY/XX). Capable of replacing a phenylalanine with valine, c.1759T>G alters a conserved position across the sixth transmembrane domain of vertebrate FSHRs. Amino acid substitutions in this position in vertebrates are frequently associated with constant receptor activation and consequently with FSH/FSHR signaling alteration; thus, indicating a potential role of fshr as an MKR of SD [ABSTRACT FROM AUTHOR]

Published
2021
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9. Quantitative trait loci on LGs 9 and 14 affect the reproductive interaction between two Oreochromis species, O. niloticusand O. aureus

Author

Shirak, Andrey, Zak, Tatyana, Dor, Lior, Benet-Perlberg, Ayana, Weller, Joel, Ron, Micha, and Seroussi, Eyal

Abstract

Effective farming of tilapia requires all-male culture, characterized by uniformity and high growth rate. Males of O. aureus(Oa) and females of O. niloticus(On) produce all-male offspring, but there is a behavioral reproductive barrier between the two species that prevents mass production. In crosses between Oaand Onbroodstocks, few hybrid females are attracted to the Oamale nests (denoted responders), and if they harbor the Onalleles for the sex determination (SD) sites on linkage groups (LGs) 1, 3, and 23, all-male progeny are produced. Yet, without controlling for the alleles underlying SD, the parental stocks gradually lose their capability for all-male production. Hypothesizing that marker-assisted selection for female responders would allow production of sustainable broodstocks, we applied genotyping-by-sequencing to generate 4983 informative SNPs from 13 responding and 28 non-responding females from two full-sib families. Accounting for multiple comparisons in a genome-wide association study, seven SNPs met a false discovery rate of 0.061. Lowest nominal probabilities were on LGs 9 and 14, for which microsatellite DNA markers were designed within the candidate genes PTGDSLand CASRL, respectively. By increasing the sample size to 22 responders and 47 non-responders and by genotyping additional established microsatellites, we confirmed the association of these LGs with female responsiveness. The combined effects of microsatellites GM171and CARSL-LOC100690618on LGs 9 and 14 explained 37% of the phenotypic variance of reproductive interaction (p< 0.0001). Based on these findings, we propose a strategy for mass production of all-male tilapia hybrids through selection for genomic loci affecting SD and female responsiveness.

Published
2019
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10. Identification of male-specific amh duplication, sexually differentially expressed genes and microRNAs at early embryonic development of Nile tilapia (Oreochromis niloticus)

Author

Eshel, Orly, primary, Shirak, Andrey, additional, Dor, Lior, additional, Band, Mark, additional, Zak, Tatyana, additional, Markovich-Gordon, Michal, additional, Chalifa-Caspi, Vered, additional, Feldmesser, Esther, additional, Weller, Joel I, additional, Seroussi, Eyal, additional, Hulata, Gideon, additional, and Ron, Micha, additional

Published
2014
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