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9. First outbreak of norovirus in Albania

Author

Donia, Dt, Kota, M, Leno, L, Ylli, A, Cenko, F, and Divizia, M

Subjects
Feces, Genotype, Caliciviridae Infections, Humans, Norovirus, Albania, Reverse Transcriptase Polymerase Chain Reaction, Gastroenteritis, Disease Outbreaks, Water Microbiology, Settore MED/42 - Igiene Generale e Applicata
Published
2011

10. Surveillance of adenoviruses and noroviruses in European recreational waters

Author

Wyn Jones, A, Carducci, A, Cook, N, D’Agostino, M, Divizia, M, Fleischer, J, Gantzer, C, Gawler, A, Girones, R, Hoeller, C, De Roda Husman, A, Kay, D, Kozyra, I, Lopez Pila, J, Muscillo, M, Sao José Nascimento, M, Papageorgiou, G, Rutjes, S, Sellwood, J, Szewzyk, R, Wyer, M, significant contributions by Bofill Mash, S, Clementeh, P, Donia, Dt, Duarten, A, Gräberl, I, Hollisterp, W, Huberi, S, Iaconellim, M, La Rosam, G, Cuvelierk, B, Orgorzalyf, L, Pissarides, N, Rosannad, G, Salagnonc, E, Schneidere, O, Docters van Leeuwenj, A, Veranib, M, Wildegm, S, Universitat de Barcelona, University of Pisa - Università di Pisa, Laboratoire de Chimie Physique et Microbiologie pour l'Environnement (LCPME), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), University of Barcelona, Ecole Polytechnique, Chemical Engineering Department, CRASP, École Polytechnique de Montréal (EPM), and The National Institute for Medical Research

Subjects
Veterinary medicine, Adenoviruses, Bathing, Settore MED/42 - Igiene Generale e Applicata, viruses, Noroviruses, Fresh Water, 010501 environmental sciences, 01 natural sciences, law.invention, Recreational water, law, Adenovirus, Waste Management and Disposal, ComputingMilieux_MISCELLANEOUS, Polymerase chain reaction, Water Science and Technology, [SDV.EE]Life Sciences [q-bio]/Ecology, environment, Infectivity, 0303 health sciences, Reverse Transcriptase Polymerase Chain Reaction, Ecological Modeling, adenoviruses, noroviruses, bathing water, river water, sea water, Pollution, 6. Clean water, 3. Good health, River water, Europe, Water quality, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Qualitat de l'aigua, Water Microbiology, Europa, Environmental Monitoring, Environmental Engineering, Frequency of occurrence, Context (language use), Biology, Article, Bathing water quality, Adenoviridae, 03 medical and health sciences, Bathing water, Sea water, Seawater, 14. Life underwater, Aigua dolça, 0105 earth and related environmental sciences, Civil and Structural Engineering, 030306 microbiology, Fresh water, Norovirus, Mean value, Environmental engineering, Outbreak, Aigua de mar, Recreation
Abstract

Exposure to human pathogenic viruses in recreational waters has been shown to cause disease outbreaks. In the context of Article 14 of the revised European Bathing Waters Directive 2006/7/EC (rBWD, CEU, 2006) a Europe-wide surveillance study was carried out to determine the frequency of occurrence of two human enteric viruses in recreational waters. Adenoviruses were selected based on their near-universal shedding and environmental survival, and noroviruses (NoV) selected as being the most prevalent gastroenteritis agent worldwide. Concentration of marine and freshwater samples was done by adsorption/elution followed by molecular detection by (RT)-PCR. Out of 1410 samples, 553 (39.2%) were positive for one or more of the target viruses. Adenoviruses, detected in 36.4% of samples, were more prevalent than noroviruses (9.4%), with 3.5% GI and 6.2% GII, some samples being positive for both GI and GII. Of 513 human adenovirus-positive samples, 63 (12.3%) were also norovirus-positive, whereas 69 (7.7%) norovirus-positive samples were adenovirus-negative. More freshwater samples than marine water samples were virus-positive. Out of a small selection of samples tested for adenovirus infectivity, approximately one-quarter were positive. Sixty percent of 132 nested-PCR adenovirus-positive samples analysed by quantitative PCR gave a mean value of over 3000 genome copies per L of water. The simultaneous detection of infectious adenovirus and of adenovirus and NoV by (RT)PCR suggests that the presence of infectious viruses in recreational waters may constitute a public health risk upon exposure. These studies support the case for considering adenoviruses as an indicator of bathing water quality.

Published
2011
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13. A strategy to increase the specificity of Syber Green I qRT-PCR in hepatitis A detection

Author

Donia, Dt, Petrinca, A, and Divizia, M

Subjects
Reverse Transcriptase Polymerase Chain Reaction, Settore MED/42 - Igiene Generale e Applicata, Sensitivity and Specificity, Humans, Hepatitis A virus, DNA Primers, Hepatitis A, Fluorescent Dyes, specificity, qrt-pcr, hairpin primer, hav, primer-dimers, syber green i, syber green i, qrt-pcr, specificity, hairpin primer, primer-dimers, hav
Published
2010

16. Possibile utilizzo di nanoparticelle in virologia

Author

Divizia, M, Donia, Dt, and Gabrieli, R

Subjects
virus enterici, Settore MED/42 - Igiene Generale e Applicata, nanotubi di Carbonio, test molecolari, Infettività su cellule
Published
2010

17. PRIMA EPIDEMIA DA NOROVIRUS IN ALBANIA

Author

Donia, Dt, Kota, M, Leno, L, Luzati, A, Bare, V, Ylli, A, Cenko, F, and Divizia, M

Subjects
Settore MED/42 - Igiene Generale e Applicata
Published
2010

28. Detection and genotypic characterization of Giardia cysts in water samples

Author

DI CAVE, D, Berrilli, F, Orecchia, P, Mecozzi, B, Donia, Dt, Bonadonna, L, Briancesco, R, and Divizia, M

Subjects
Settore VET/06 - Parassitologia e Malattie Parassitarie degli Animali, Genotype, Settore MED/42 - Igiene Generale e Applicata, Giardia, Waste Disposal, Rome, Water Pollution, Oocysts, Fluorescent Antibody Technique, Fresh Water, Polymerase Chain Reaction, Waste Disposal, Fluid, Animals, Humans, Fluid
Abstract

Water samples were collected from a wastewater treatment plant and from two lakes in the vicinity of Rome (Italy) for detection and genotypic characterization of Giardia. All wastewater samples and all but two of the lake samples were scored by immunofluorescence as positive for Giardia. In addition, Giardia cysts were detected, by nested PCR, in all of the examined samples. Analysis of the SSU-rRNA gene sequences showed that all Giardia isolates belonged to Assemblage A. The presence of Assemblage A genotype in wastewater and surface bathing water represents an important public health concern. In fact, isolates showing this genotype are considered to be potential human pathogens and also have zoonotic implications.

Published
2007

37. Batteriofagi

Author

Donia, Dt, Divizia, M, and Panà, A

Subjects
Settore MED/42 - Igiene Generale e Applicata
Published
2003

38. I batteriofagi

Author

Donia, Dt

Subjects
Settore MED/42 - Igiene Generale e Applicata
Published
2003

39. Fate of bacterial indicators, viruses and protozoan parasites in a wastewater multi-component treatment system

Author

Bonadonna, L, Briancesco, R, Cataldo, C, Divizia, M, Donia, Dt, and Panà, A

Subjects
Spores, Sewage, Clostridium perfringens, Giardia, Settore MED/42 - Igiene Generale e Applicata, Waste Disposal, Bacterial, Cryptosporidium, Environmental Monitoring, Biodegradation, Environmental, Enterococcus, Viruses, Waste Management, Animals, Waste Disposal, Fluid, Spores, Bacterial, Environmental, Biodegradation, Fluid
Published
2002

44. Water quality control of 2 swimming pools in Rome. Evaluation of the bacteriophage parameter [Controllo qualitativo dell'acqua di due piscine riabilitative di Roma. Valutazione del parametro 'batteriofagi'.]

Author

Donia, Dt, Divizia, M, della Sallette Mattiacci, M, and Pana, A

Subjects
Microbiological Techniques, Bacteria, isolation and purification, Settore MED/42 - Igiene Generale e Applicata, microbiology, Rome, article, standard, bacterium, swimming pool, Swimming Pools, bacteriophage, Italy, Bacteriophages, microbiological examination, Water Microbiology, comparative study
Published
2000

47. [The molecular biology characterization of a poliomyelitis epidemic in Albania]

Author

Donia, Dt, Ruscio, V, Divizia, M, and Palombi, L

Subjects
Poliovirus, Molecular Epidemiology, Feces, Genome, Reverse Transcriptase Polymerase Chain Reaction, Settore MED/42 - Igiene Generale e Applicata, Poliomyelitis, Humans, Genome, Viral, Albania, Disease Outbreaks, RNA, Viral, RNA, Viral
Published
1999

48. Caratterizzazione tramite biologia molecolare di una epidemia di poliomielite in Albania.(The molecular biology characterization of a poliomyelitis epidemic in Albania)

Author

Donia, Dt, Ruscio, V, Divizia, M, and Palombi, L

Subjects
Genome, isolation and purification, Reverse Transcriptase Polymerase Chain Reaction, Epidemiology, Settore MED/42 - Igiene Generale e Applicata, article, Molecular, virus RNA, Albania, epidemic, epidemiology, feces, genetics, human, poliomyelitis, Poliomyelitis virus, reverse transcription polymerase chain reaction, virology, virus genome, Disease Outbreaks, Epidemiology, Molecular, Feces, Genome, Viral, Humans, Poliomyelitis, Poliovirus, RNA, Viral, RNA, Viral
Published
1999

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