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Abstract With the exploration, development, and research of deep‐sea resources, there is an urgent need for long‐term and continuous observation data of the deep‐sea seabed boundary layer. The traditional method of deep‐sea seabed survey and sampling based on scientific research vessels has the discontinuity of observation data in space and time scales. There are some problems in the seabed in situ observation method based on the seabed observation network for low mobility and high operation and maintenance costs, restricting the in‐depth understanding of the dynamic change process of the deep‐sea floor. To solve the above problems, an open and modular data acquisition control system was designed based on an embedded system and signal processing technology. In terms of the physical, chemical, geological, and ecosystem characteristics of the seafloor or near the seafloor boundary layer, various functional sensors and instrumentation were matched to form an independent underwater integrated measurement or experimental device, eventually realizing in situ multiparameter and long‐time series observations of the seafloor. The system data acquisition and control test were completed through laboratory experiments, which verified the feasibility of the system design. The research showed important theoretical and technical reference significance for the exploration and development of resources in the submarine boundary layer and the promotion of deep‐sea scientific research.

Nuclear magnetic resonance (NMR) measurement could provide information during the formation and dissociation of gas hydrate on the pore structure properties which are of great important to understand the gas production behavior. In this study, the in–situ NMR and other well logging data achieved during the natural gas hydrate drilling program were used to study the pore size and hydrate formation properties in the Shenhu area, South China Sea by comparing transverse magnetic relaxation time (T2) distributions between the gas hydrate zone and the upper water–saturated zone whose properties represent the virgin status. Compared with those of the upper water–saturated layer, increasing resistivity, higher–than–baseline shear modulus, less–than–density–porosity NMR porosity, shortening T2 peak time, reducing amplitude and covered area of T2 distributions and decreasing water–filling pore size denote the association of hydrate. Based on eight bin porosities distribution derived from user-defined time intervals of the T2 distribution for researching the hydrate formation characteristic in different pore size, macro–pores (T2 ≥ 10 ms, rc≥ 0.9 μm), meso–pores (3 ms ≤ T2 < 10 ms, 0.27 μm ≤rc

The sources and sinks of dissolved CH4 in offshore waters are becoming diversified with the rapid increase in human activities. The concentration and air–sea exchange flux of dissolved CH4 present new characteristics of more intense spatiotemporal evolution, and the contribution to atmospheric CH4 continues to increase. Herein, a new model based on navigable air–sea exchange flux observations was proposed, which replaced the traditional station-based sampling analysis and testing method, realizing the synchronous measurement of methane in the atmosphere and surface seawater carried by ships. Based on the Marine Geological Survey project of the China Geological Survey, comprehensive environmental surveys were conducted in April 2018, September 2018, and June 2019 in the Qiongdongnan area in the northern part of the South China Sea, and the dissolved methane content in the sea surface atmosphere and surface seawaters in 2019 were simultaneously obtained. The methane exchange flux ranges of the southeastern sea area were calculated as −0.001~−0.0023 μmol·m−2·d−1 and −0.00164~−0.00395 μmol·m−2·d−1 by using the Liss and Merlivat formula (LM86), the Wanninkhof formula (W92), and the field-measured wind speed. The feasibility of the navigational air–sea methane exchange flux observation system was proven in a sea trial, and the measurement accuracy and observation efficiency of air-sea flux were improved with the designed system, providing a new technical means for further research on multiscale air–sea interactions and global climate change.

Natural gas hydrate is easy to decompose and leak due to the changes in temperature and pressure during drilling, which causes safety accidents. Early monitoring of wellhead overflow is a practical and effective measure to prevent overflow blowouts and other accidents. Herein, a wellhead methane monitoring system for the open-circuit drilling of marine natural gas was designed. The system consisted of an overwater acoustic reception part and an underwater self-contained methane monitoring part, matching the construction environment of marine natural gas hydrate exploitation. Compared with the existing gas logging technology (measurement while drilling), the monitoring and early warning of wellhead methane content were realized at all stages of drilling, casing running, cementing, completion and fracturing in the process of natural gas hydrate exploitation. System communication and data acquisition tests were completed at different water depths through sea trials, which verified the effectiveness of the system design. The research results provide important theoretical and technical implications for promoting the development of early spill monitoring technology at the wellhead of open-circuit drilling for marine gas hydrates.

TLR signaling is essential to innate immunity against microbial invaders and must be tightly controlled. We have previously shown that TLR9 undergoes proteolytic cleavage processing by lysosomal proteases to generate two distinct fragments. The C-terminal cleavage product plays a critical role in activating TLR9 signaling; however, the precise role of the N-terminal fragment, which remains in lysosomes, in the TLR9 response is still unclear. In this article, we report that the N-terminal cleavage product negatively regulates TLR9 signaling. Notably, the N-terminal fragment promotes the aspartic protease-mediated degradation of the C-terminal fragment in endolysosomes. Furthermore, the N-terminal TLR9 fragment physically interacts with the C-terminal product, thereby inhibiting the formation of homodimers of the C-terminal fragment; this suggests that the monomeric C-terminal product is more susceptible to attack by aspartic proteases. Together, these results suggest that the N-terminal TLR9 proteolytic cleavage product is a negative self-regulator that prevents excessive TLR9 signaling activity. [ABSTRACT FROM AUTHOR]