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Prevention of African swine fever, a disease caused by African swine fever virus (ASFV), requires maintenance of high biosecurity standards, which principally relies on disinfection. Finding the perfect disinfectant against ASFV is difficult because of the lack of relevant data. Therefore, we aimed to find the most effective disinfectant and to optimise its concentration as well as contact time to confirm the viricidal effect against ASFV in vitro. We evaluated the viricidal activity of three concentrations each of six common disinfectants against ASFV using immersion disinfection assay (IDA) and spray disinfection assay (SDA); the concentrations of these disinfectants at which complete viral inactivation occurred were almost same as the manufacturer-recommended concentrations, but the exposure times for viral inactivation are different. The following disinfectants (assay: concentration, exposure time) showed complete inactivation: iodine and acid mixed solution (IDA/SDA: 0.5%, 10 min); compound potassium peroxymonosulfate (IDA: 0.25%, 30 min; SDA: 0.25%, 60 min); citric acid (IDA: 0.25%, 60 min; SDA: 0.5%, 60 min); sodium dichloroisocyanurate (IDA: 0.125%, 60 min; SDA: 0.25%, 60 min); and glutaral ang deciquam (IDA/SDA: 0.2%, 60 min); and deciquam (IDA/SDA: 0.5%, 60 min). However, in the presence of organic material contamination, disinfectants did not show a marked inactivation effect. Therefore, disinfection procedures should be performed in two steps: thorough mechanical cleaning followed by application of disinfectant. In conclusion, all the tested disinfectants can inactivate ASFV; these can be used as alternative disinfectants to enhance biosecurity.

Objective: Currently available evidence regarding the association between collagen type XII α1 (COL12A1) polymorphism and risk of anterior cruciate ligament rupture (ACLR) remains elusive. The aim of our present study was to assess the association between COL12A1 rs970547 polymorphism and ACLR risk.Methods: Five online databases, namely, PubMed, EMBASE, ISI Web of Science, CENTRAL, and CNKI, were searched from their inception data up to December 2020 to identify relative observational studies. The methodological quality of each individual study was evaluated using the Newcastle-Ottawa Scale (NOS). The “model-free approach” was employed to estimate the magnitude of effect of COL12A1 rs970547 polymorphism on ACLR, and the association was expressed using odds ratio (OR) and its associated 95% confidence interval (95% CI). Subgroup analysis was performed by ethnicity and sex of included subjects.Results: Eight studies involving 1,477 subjects with ACLR and 100,439 healthy controls were finally included in our study. The methodological quality of included studies was deemed moderate to high based on NOS scores. The “model-free” approach suggested no genotype differences between ACLR and healthy control for the rs970547 polymorphism, but we still used the allele model to present the combined data. Under the random-effect model, there was no significant difference in the frequency of effecting allele between ACLR and control (OR: 0.91, 95% CI 0.77, 1.08; p = 0.28). Stratified analysis by sex and ethnicity also showed no difference in allele frequency.Conclusion: The findings of this current meta-analysis suggested that rs970547 was not associated with ACLR risk in male, female, and the overall population among Asians or Caucasians.

Dalbergia hainanensis is a vulnerably endangered tree species with high economic and ornamental value. The complete chloroplast (cp) genome of D. hainanensis was determined to provide the underlying information for genetic and conservation studies and explore possible phylogenetic relationship within leguminous family in this study by next generation Illumina pair-end sequencing dataset. The whole genome was 156,073 bp in length and contains a pair of 182,574 bp inverted repeats regions, which were separated by a large single copy region and a small single copy region of 85,831 and 18,838 bp in length, respectively. The cp genome contained 131 genes, including 86 protein-coding genes, 36 tRNAs and 8 rRNAs. The overall GC content of the whole genome was 36.09%. A neighbour-joining phylogenetic analysis suggested a close relationship between D. hainanensis and Arachis hypogaea.

In polyglutamine (PolyQ) diseases, mutant proteins cause not only neurological problems but also peripheral tissue abnormalities. Among all systemic damages, skeletal muscle dystrophy is the severest. Previously by studying knock-in (KI) mouse models of spinal cerebellar ataxia 17 (SCA17), it was found that mutant TATA box binding protein (TBP) decreases its interaction with myogenic differentiation antigen, thus reducing the expression of skeletal muscle structural proteins and resulting in muscle degeneration. In this paper, the role of mutant TBP in myogenesis was investigated. Single myofibers were isolated from tibialis anterior muscles of wild type (WT) and SCA17KI mice. The 1TBP18 staining confirmed the expression of mutant TBP in muscle satellite cells in SCA17KI mice. In the BaCl2-induced TA muscle injury, H&E cross-section staining showed no significant change in myofibril size before and after BaCl2 treatment, and there was no significant difference in centralized nuclei between WT and SCA17KI mice, suggesting that mutant TBP had no significant effect on muscle regeneration. In the cultured primary myoblasts from WT and SCA17KI mice in vitro, representative BrdU immunostaining showed no significant difference in proliferation of muscle satellite cells. The primary myoblasts were then induced to differentiate and immunostained for eMyHC, and the staining showed there was no significant difference in differentiation of primary myoblasts between WT and SCA1KI mice. Our findings confirmed that mutant TBP had no significant effect on myogenesis.

In this paper, a regional traditional sport industry upgrading model with pure strategy evolution game is discussed. The quantitative analysis of traditional sport industries upgrading model are obtained. Our results propose countermeasures for traditional sport industries upgrading.

Previous studies have suggested that endoplasmic reticulum stress (ERS) is one of the mechanisms responsible for the pathogenesis of diabetic nephropathy (DN). Histone acetylation modification can regulate the transcription of genes and is involved in the regulation of ERS. Valproate (VPA), a nonselective histone deacetylase inhibitor, has been reported to have a protective role in kidney tissue injury, however, whether VPA can prevent DN remains to be elucidated. In the present study, it was found that VPA increases the expression of glucose-regulated protein (GRP78) and reduces the protein expression of C/EBP-homologous protein (CHOP), growth arrest and DNA-damage-inducible gene 153 and caspase-12 in a rat model of DN. VPA can reduce renal cell apoptosis and alleviate proteinuria and alterations in serum creatinine. VPA also upregulates the acetylation level of histone H4 in the promoter of GRP78 and downregulates the acetylation level of histone H4 in the promoter of CHOP. Collectively, the data indicate that VPA can relieve ERS and reduce renal cell apoptosis, and thus attenuate renal injury in a rat model of DN by regulating the acetylation level of histone H4 in ERS-associated protein promoters.

Internet technology as the cutting-edge of the current information technology, it has penetrated into every aspect of human life, more and more government agencies, enterprises are using Internet to improve their office efficiency and to establish new business operation model, in order to improve its reaction ability in fierce competition. Internet has brought convenience for people to work, while the security problem of network also will follow. The internal network not only needs to ensure the security of their data, but also needs to exchange data with the external network, then by adopting network security isolation system which takes gatekeeper technology as its core technology, so that we can ensure that internal network and external network will have physical isolation at any time, meanwhile, according to business needs, it also has multiple forms of information and data’s exchanges.

Online homework correcting system based on ASP.NET is an online homework correcting system based on B/S mode, which can provide online work service for teachers and students to improve school teaching level. The characteristics of the system are making the traditional pattern of homework assignments and correcting networked, strengthening the connection between teacher and student, and also improving the teaching efficiency. How to use ASP.NET technology to build an online job submission and the process of correcting system, this article discusses the concept and characteristics of B/S mode, the superiority of ASP.NET and the analysis of general homework system. The system mainly completes the homework uploading and the teacher corrects for homework, it has high stability and simple operation.

The arrival of information age means the change of teaching and learning methods, with the development of computer technology, multimedia technology and network technology, the teaching mode of interaction through the network are also popular to carry out gradually. Changes have taken place in teaching methods, teaching means, teaching form and teaching idea through the application of computer in teaching. Distance education, online examination system, the development of online learning system and online homework system greatly promote the development of the educational business and also bring a huge change to modern education, profoundly affected the modern teaching domain. Applied-information technology plays an increasingly important role in contemporary teaching. To study the application of applied-information technology in teaching research has important theoretical and practical significance.At the same time of computer aided teaching, the arrangement and correcting homework are particularly important for checking the teaching achievements and the degree of students what have mastered.

With the rapid development of network technology, computer networks and the Internet have become an important force in promoting social development, many enterprises and government agencies have been or are preparing to build its own internal network as the center of the entire information system, through a variety of methods accessed internal network to the Internet, thereby greatly enhanced their information acquisition and processing capabilities. Isolated net gap is an enterprise-level physical isolation technology; its generation is to solve the problems that exist in network security.

As the use of computers become more widely, its intrusion security has become a key issue. In order to solve this problem, this article proposes a analysis research on AC algorithm for the detection of network intrusion. Through the research on the patterns of network intrusion, the AC algorithm for the detection of network intrusion is proposed and analyzed. Simulation results show that the proposed AC algorithm of network intrusion detection has a good efficiency, higher accuracy and efficient use of resources.

In this study, one-step electrochemical polymerized method for the fabrication of poly( o -aminophenol)/multi-walled carbon nanotubes (PoAP/MWCNTs) composite film was developed for the quantitative determination of levofloxacin. Scanning electron microscopy and electrochemical methods were used to characterize the PoAP/MWCNTs nanocomposite film. The sensor fabricated from the nanocomposite film showed good stability and enhanced electrocatalytic ability for the detection of levofloxacin. The large current response of levofloxacin on PoAP/MWCNTs composite film-modified electrode could be ascribed to the synergistic effect of the electrocatalytic property of PoAP and MWCNTs. The oxidative peak current increased linearly with the concentration of levofloxacin in the range of 3.0–200 μM by differential pulse voltammetry, and the detection limit was 1.0 μM (S/N = 3). The proposed modified electrode also can be successfully applied to the determination of levofloxacin hydrochloride in pharmaceutical formulations.

The paper presents a contactless mobile payment method based on security TF card and NFC technology, with which we can use the security TF card, instead of a special SIM card that support SWP protocol, as a security module to store the user’s important information (e.g. transaction data) in mobile payment. And because security TF card can be distributed by non-telecom operators, the method can obtain a more extensive support by units and institutions other than telecom operators. then it is meaningful to the development of NFC technology.

A simple and highly sensitive electrochemical method was developed for the determination of trace-level luteolin, based on the multi-walled carbon nanotubes (MWNTs) modified glassy carbon electrode (GCE). The electrochemical behaviours of luteolin indicate that MWNTs modified glassy carbon electrode (MWNTs/GCE) can greatly enhance the electrocatalytic activity towards the redox of luteolin. It leads to a considerable improvement of the redox peak current for luteolin and allows the development of a highly sensitive voltammetric sensor for the determination of luteolin. A series of experimental parameters including the pH of supporting electrolyte, accumulation potential and time were optimised. The results showed that the oxidative peak currents increased linearly with the concentration of luteolin in the range of 2.0 × 10 −10 to 3.0 × 10 −9 M, with a detection limit of 6.0 × 10 −11 M ( S / N = 3). The analytical performance of this sensor has been evaluated for detection of luteolin in peanut hulls as a real sample.

A biosensor based on bovine serum albumin (BSA) and poly- o -phenylenediamine (PoPD)/carbon-coated nickel (C-Ni) nanobiocomposite film modified electrode has been developed to study the interaction of BSA with papaverine (PAP). The well-dispersed C-Ni nanoparticles were dripped onto the glassy carbon electrode (GCE) surface firstly, and PoPD films were subsequently electropolymerized by cyclic voltammetry (CV) to prepare PoPD/C-Ni/GCE. Finally, the BSA was easily immobilized on the PoPD films via electrostatic adsorption. The morphology and the electrochemical properties of the fabricated composite electrodes were examined by scanning electron microscope (SEM) and electrochemical impedance spectroscopy (EIS), respectively. The interaction of PAP with BSA was monitored by differential pulse voltammetry (DPV), using PoPD as the electrochemical indicator. The binding constant ( K ), obtained by DPV, was 1.7 × 10 4 L/mol, which was consistent with the fluorescence analysis. This constructed biosensor also exhibited a fine linear correlation with PAP concentration range of 2.5 × 10 −9 –4.5 × 10 −5 mol/L and a detection limit of 8.3 × 10 −10 mol/L was achieved by DPV.

A glassy carbon electrode was prepared that was coated with a composite film containing electropolymerized poly(amidosulfonic acid) and multi-walled carbon nano- tubes. It was used to study the electrochemical response of procaine by differential pulse voltammetry. The results indicate that the electrode exhibits a remarkable improve- ment in the oxidation peak of procaine, and this led to a simple and sensitive method for the electroanalytical determination of procaine. The peak current is proportional to the concentration of procaine from 80 nM to 1.0 µM. The detection limit is 25 nM (S/N=3). The modified electrode was successfully applied to the direct determina- tion of procaine in pharmaceutical formulations.

A retrospective cohort study was conducted to compare fusion rates achieved by H-grafts, posterior vertebral grafts, and no graft for the surgical treatment of thoracolumbar fractures. Ninety-two patients were included in this study. The patients fell into 1 of 3 groups: those who received H-grafts (n=36), posterior vertebral grafts (n=30), and no graft (n=26). Mean follow-up was 38 months (range, 24–51 months). All operations were performed by a single senior surgeon. All patients underwent operative treatment with posterior reduction and instrumentation. Radiographic parameters, estimated blood loss, operative time, and length of hospital stay were compared among patients in the 3 graft groups. Differences were assessed using unpaired t tests. P values P P

To study the effects of 21 d -6 degrees head down bed rest (HDBR) with exercise training on dynamic posture equilibrium and motor coordination.Ten healthy young men were randomly divided into HDBR control groups and HDBR exercise groups, with 5 in each group. The subjects in the exercise group performed exercise with a bicycle ergometer in the supine position 1 h/d during bed rest. Dynamic posture equilibrium as well as isokinetic concentric muscular strength were determined before and after 21 d HD-BR.After HDBR, average dynamic proprioception score and dynamic motor coordination decreased significantly, relative peak torque (peak torque /body weights PT/BW) also decreased significantly and accompanied with increase of the peak torque ratio between hamstrings and quadriceps (H/Q) in HDBR control groups. Average dynamic proprioception score and dynamic motor coordination in the HDBR exercise groups remained above the HDBR control groups, but the H/Q ratio showed no significant change.The results showed that exercise training during 21 d -6 degrees head-down tilt bed-rest do have beneficial effects on retaining the dynamic posture equilibrium and dynamic motor coordination of the subjects after bed rest.

The regulation of foreign gene expression in Insect-Baculovirus Expression System is very complex. In this report, the effect of 5'-UTR in the expression of hGH gene in cultured Sf9 cells was examined. A 18 bp length in the end of 5'-UTR of hGH (human Growth Hormone, hGH) cDNA including a stem-loop structure was deleted by PCR. The truncated hGH cDNA, delta 1hGH was cloned in pFastBac1, named pFast-Bac-delta 1hGH. After transforming into E. coli. DH10Bac, which have a shuttle vetor-Bacmid, the delta 1hGH was integrated into Bacmid by site-specific transposition, and an expression vector, rBacmid-delta 1hGH DNA was acquired. By transfecting the cultured Sf9 cells with the recombinant expression vector DNA, pure recombinant virus, rAcV-Bac-delta 1hGH was obtained, and hGH gene was expressed. Immuno-blot and Chemiluminescent assay revealed that the expressed hGH had normal immunological activity, the amount of hGH expression level in Sf9 cell supernatant infected with rAcV-Bac-delta 1hGH containing the truncated 5'UTR was four to five times higher than that infected with rAcV-Bac-hGH.

Accurate reduction of two-dimensional curve noises is crucial to metrology and reverse engineering. In this paper we propose a set of schemes for two-dimensional curve denoising. Our approach consists of three main steps: 1) feature-based pre-smoothing of noised polylines; 2) curve partitioning of polylines into different regions; 3) two hybrid denoising schemes of arbitrary polylines with noises. Numerical experiments indicate the effectiveness of our approach against existing methods.