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This study aims to explore the mechanism of Astragali Radix-Puerariae Lobatae Radix(AP) combination in the treatment of type 2 diabetes mellitus(T2 DM) based on network pharmacology and experiment. The effective components and targets of the pair were retrieved from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP) and targets of T2 DM from each disease database. On this basis, the common targets of the medicinals and the disease were screened out. The protein-protein interaction(PPI) network was established based on STRING. Then Cytoscape 3.7.1 was employed for visualization of the common targets and the network topology analysis of key targets, followed by Gene Ontology(GO) term enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment of core targets by DAVID. Thereby, the possible molecular mechanism was unveiled. High-fat diet was combined with streptozotocin(STZ, injected into tail vein) for T2 DM rat modeling. Rats were classified into the normal group, model group, positive control group(metformin hydrochloride), AP high-dose, medium-dose, and low-dose groups. After 4 weeks of intragastric administration, serum fasting blood glucose(FBG), fasting insulin(FINS), aspartate aminotransferase(AST), alanine aminotransferase(ALT), triglyceride(TG), total cholesterol(TC), low-density lipoprotein cholesterol(LDL-C), high-density lipoprotein cholesterol(HDL-C), interleukin(IL)-6, and tumor necrosis factor(TNF)-α of rats in each group were measured. The expression of insulin receptor substrate-2(IRS-2), adenosine monophosphate-activated protein kinase(AMPK), phosphorylated AMPK(p-AMPK), glucose 6 phosphatase(G6 Pase), and phosphoenolpyruvate carboxy kinase(Pepck) in rat liver was detected by Western blot. A total of 131 core targets of the combination in the treatment of T2 DM were screened out, among which protein kinase B(AKT) 1, mitogen-activated protein kinase(MAPK) 1, TNF-α, IL-6 were more critical. KEGG enrichment analysis suggested that the combination decreased blood glucose mainly through PI3 K/AKT signaling pathway, AMPK signaling pathway, TNF signaling pathway, and MAPK signaling pathway. The levels of FBG and FINS were lower and the glycogen level was higher in the AP high-dose and medium-dose groups than in the model group. The levels of AST, ALT, TG, and LDL-C in the three AP groups and the level of TC in AP high-dose and low-dose groups decreased compared with those in the model group. Levels of IL-6 and TNF-α were lower in AP high-dose and medium-dose groups than in the model group. The expression of IRS-2, AMPK, and p-AMPK was higher and that of G6 Pase and Pepck was lower in AP high-dose group than in the model group. Thus, the combination had multi-component, multi-target, and multi-pathway characteristics in the treatment of T2 DM. It may regulate AMPK signaling pathway through IL-6 and TNF-α to influence insulin resistance, glycogen synthesis, gluconeogenesis, islet β cell transport, and inflammatory response, thereby exerting therapeutic effect on T2 DM.

Objective To examine the clinical effect of computed tomography angiography (CTA) on parameters of intracranial aneurysms in different locations and with different sizes using digital subtraction angiography (DSA) as the standard. Methods Patients with intracranial aneurysms who underwent CTA examinations at the same center and received DSA examinations within 3 days were analyzed retrospectively. The morphological parameters of the aneurysms and parent arteries were measured with these two methods. Results Mean aneurysm size and parent artery diameter were not different between CTA and DSA. The size of microaneurysms was significantly smaller with DSA than with CTA. The aneurysmal neck width was not different between CTA and DSA. DSA could clearly evaluate the relationship between the aneurysmal neck and the parent artery in all cases. However, CTA had a 90% accuracy rate of visualizing this relationship. Conclusion The accuracy rates of evaluating aneurysm size and the aneurysmal neck width and parent artery diameter are similar between CTA and DSA. A DSA examination is essential for evaluating the relationship among microaneurysms, the aneurysmal neck, and the parent artery. CTA is widely applied and more safe in clinical practice, while DSA has a better guiding effect than CTA for some complicated aneurysms.

Three kinds of carboxyfullerenes, La@C82-COOH, C60-COOH and C70-COOH, were used to modify gold electrodes. The electrochemical behavior of dopamine (DA) at the carboxyfullerenes modified electrodes was studied using the cyclic voltammetry (CV) and differential pulse voltammetry (DPV) methods. The C70-COOH modified electrodes performed the best among them. Under the optimal conditions, linear CV and DPV responses to DA concentration from 1 μmol/L to 13 μmol/L were observed, and the detection limit of DA was 1×10−2 μmol/L in the presence of 10 μmol/L uric acid (UA). The practical analytical utilities of the C70-COOH modified electrodes were demonstrated by the determination of DA HCl in the real samples of dopamine hydrochloride injection (DHI). Moreover, the modified electrodes exhibited good reproducibility and sensitivity, demonstrating its feasibility for analytical purpose.

In this paper, a thermo- and pH-responsive microfiltration membrane was prepared based on three-dimensional (3D) inverse colloidal crystals (ICC). To manufacture the smart ICC membrane, the typical thermo-responsive N-isopropylacrylamide (NIPAM) and pH-responsive methacrylic acid (MAA) were polymerized inside silica colloidal crystals. The smart ICC membranes were characterized by SEM, IR and contact angle measurements. Moreover, the permeability of smart microfiltration membrane was carried out by the KCl diffusion tests. The result showed that effective diameter of the polymer ICC membrane can be reversible tuned by temperature and pH. Besides, the functional ICC membrane showed outstanding temperature- and pH-responsive gating property, which was applied to separate particles of different sizes. The savvy environment-responsive gating membranes have potential uses in filtration, separation, purification, sensor and other applications.

Objective To explore the efficacy of adenomyosis treated by acupuncture. Methods Acupuncture combined with TDP was applied to the adenomyosis patients. Taichōng ( LR 3), Taixī ( KI 3), Sānyīnjiāo ( SP 6) and Hegŭ ( LI 4) were selected and TDP was applied to the patients with waist and abdominal chill. The efficacy was evaluated. Results Twenty-three cases were treated, 16 cases were cured, 7 cases were improved and 0 cases were ineffective, the total effective rate was 100%. Conclusion Acupuncture combined with TDP has sound effect in the treatment of adenomyosis.

To investigate the adequate luting cements for zirconia ceramics to dentin.Blocks of sintered zirconia ceramics were randomly divided into 4 groups with 8 slices in each. After saliva immersion,airborne-particle abraded ceramic specimens were cleaned with phosphoric acid gel(containing 35% phosphoric acid) and then bonded to dentin with these four kinds of luting cements. After preserved in 37 degrees centigrade distilled water for 24 hours, the shear bonding strength of these specimens was tested and the data was analyzed with SPSS12.0 software package.The Multilink Automix could attain the highest shear bonding strength and the 3M RelyXTM Unicem AplicapTM could attain higher shear bonding strength, which were both significantly higher than in the Tokuso Ionomer and Shofu Polycarboxylate Cement groups(P0.05).Total etching resin luting cement is an ideal option to the bonding of zirconia ceramics and can provide a strong bonding.

To evaluate the efficiency of self-made paste on gingival retraction in dogs as well as its potential clinical application.Forty teeth from two mature dogs were prepared with 0.5 mm wide shoulder in the lip and buccal sides of the teeth, and then divided into four groups randomly. The gingival grooves of the four groups were filled with self-made colloid paste, Expasyl gingival retraction paste, gingival retraction cord, and medicated (15.5% Fe2SO4 solution) gingival retraction cord, respectively. The gingival grooves models before and after gingival retraction were analyzed using the image precise mapper instrument; the gingival width and height of the gingival groove were compared using SAS6.12 software package.The changes of gingival width of the gingival groove before and after gingival retraction were not significant between the four groups. However, the gingival height of the gingival groove in the medicated gingival retraction cord group increased significantly [(0.423 + or - 0.348) mm before gingival retraction and (0.623 + or - 0.278) mm after gingival retraction] compared with the other three groups.The self-made colloid paste for gingival retraction is efficient like Expasyl gingival retraction paste and gingival retraction cord with potential clinical application, while the medicated gingival retraction cord can induce gingival shrinkage. Supported by Research Fund of Science and Technology Bureau of Huangpu District,Shanghai.

To observe the effect of electroacupuncture (EA) of different acupoints on blood pressure (BP), plasma angiotensin (Ang) II, aldosterone (ALD) and atrial natriuretic peptide (ANP) of SHR, so as to observe the relative specificity of effects of acupoints.A total of 60 SHR were randomly divided into model, Quchi (LI 11), Zusanli (ST 36), Shenmen (HT 7), Jianyu (LI 15) and non-acupoint groups, with 10 cases in each. Other 10 Wistar rats were composed of normal group. EA (2 Hz, 2 mA) was applied to LI 11, ST 36, HT 7, LI 15 and non-acupoint for 30 min, followed by measuring BP with BP-6 system. Plasma Ang II, ALD and ANP contents were assayed by radioimmunoassay.Compared with control group and pre-treatment, both systolic pressure (SP) and diastolic pressure (DP) of Quchi (LI 11), Zusanli (ST 36) and Shenmen (HT 7) groups lowered significantly (P0.05). No significant differences were found in the SP and DP of Jianyu (LI 15) and non-acupoint groups (P0.05). After EA, the SP of Quchi (LI 11) and Shenmen (HT 7) groups were significantly lower than that of Jianyu (LI 15) and non-acupoint groups (P0.05); and the DP of Quchi (LI 11), Zusanli (ST 36) and Shenmen (HT 7) groups were markedly lower than that of Jianyu (LI 15) and non-acupoint groups (P0.05). Compared with normal group, the contents of plasma Ang II and ANP of model group decreased significantly, and plasma ALD level of model group increased remarkably (P0.05). Compared with model group, plasma AngII levels in Quchi (LI 11), Zusanli (ST 36) and Shenmen (HT 7) groups and ALD in Zusanli (ST 36) and Shenmen (HT 7) groups lowered significantly (P0.05), and plasma ANP content in Shenmen (HT 7) group increased obviously (P0.05).EA of LI 11, HT 7 and ST 36 can effectively lowered both DP and SP in SHR, which may be closely related to its effects in regulating the contents of Ang II , ALD and ANP.

To evaluate the stress distribution of mandibular second premolar restored with different shapes and diameters fiber post-core.A load of 200N was distributed to the finite element model of mandibular second premolar restored with three different shapes (column post, cone post and trapezium post) and three different diameters(1/2,1/3 and 1/4 of root diameter) fiber post-core in vertical and lateral ( 45 degrees ) direction, and software of "Nastran" was applied to analyze the three-dimensional finite element distribution of stress respectively.Irrespective of the shapes, there was no significant change of stress distribution with the increase of post diameter. Under lateral load, the cone post and trapezium post created the least increased range of maximum stress than that under the vertical load.Cone and trapezium fiber posts are the more ideal designs to restore the residual crowns and roots.

To investigate the effect of silica coating by sol-gel process on bonding strength of zirconia ceramics to dentin.Blocks of sintered zirconia ceramics were cut and randomly divided into 4 groups,16 slices in each group. Each group was subject to one of the 4 kinds of surface treatment (control group, sandblasting, sandblasting +silicone, sandblasting + silica coating + silicone) and then bonded to dentin with resin cement. After preservation in 37 degrees centigrade distilled water for 24 hours, the shear bonding strength of these specimens was tested and the data was analyzed with SAS6.12 software package for analysis of variance. The surface modality of the ceramics was observed under scanning electron microscopy (SEM).The group of sandblasting+ silica coating + silicone attained the highest shear bonding strength, which was significantly different from the other groups(P=0.000);There was no significant difference between the sandblasting and sandblasting + silicone group (P=0.827), which was significantly different from the control group(P=0.001).Silica coating by sol-gel process, coupled with silicone, can significantly increase the bonding strength of zirconia ceramics to dentin.

To study the effect of Streptococcus sanguis on corrosion resistance of magnetic retainers in which are encapsulated with stainless steel artificial saliva.A magnetic retainer was put in one culture flask,which was filled with artificial saliva.Ten of them were added Streptococcus sanguis suspension,the others were added PBS as control.The culture flasks were cultivated in incubator(36 degrees centigrade). 5 ml suspension was dislodged from each flask at the 3rd, 10th and 20th day. The quantity of Fe(3+) and Mn(2+) was detected.Statistical analysis of paired t test was performed with SPSS10.0 software package.When Streptococcus sanguis existed, the quantity of Fe(3+) and Mn(2+) was significantly higher than that in the control (P0.01).The existence of Streptococcus sanguis destroys the corrosion resistance of magnetic retainers remarkably.

To study the bonding strength of zirconia ceramics with 4 kinds of luting cement materials.Blocks of sintered zirconia ceramics were cut and randomly divided into 4 groups with 16 slices in each group. They were treated with sandblasting and bonded with 4 kinds of luting cements respectively. After preserved in 37 degrees C distilled-water for 24 hours and 30 days, the shear bonding strength of these specimens was tested and the data were analyzed by SAS6.12 software package and bond section were observed by scanning electron microscope.Two-way ANOVA revealed that the group of PanaviaF could attain the highest shear bonding strength: (34.7+/-3.44) MPa (after 24 hours), (31.5+/-3.44) MPa (after 1 month), which was significantly different from other treatment methods (P0.01). The initial shear bonding strength of the groups of resin-reinforced glass ionomer was (15.5+/-2.71) MPa, (16.0+/-1.77) MPa (after 24 hours) but dropped markedly to (6.80+/-1.24) MPa, (3.38+/-2.32) MPa after 30 days (P0.05).Resin luting cement containing phosphate monomer (MDP) can provide zirconia ceramics a strong and long-lasting bonding. Resin-reinforced glass ionomer can get good bonding strength too, but can't last long.

To study the influence on the abutment movement with three kinds of precision attachments designed for distal-extension removable partial dentures (RPDs).Snap attachment, Ceka attachment, Mini-SG attachment were used to retain RPDs. Then food chip was masticated between the upper and lower dentitions on the attachments side. Placement of abutment was measured by LB-72 high-sensitive-laser-traneferring-detector and the data were statistically analyzed for random block analysis of variance.The displacement, inclination, and torsion of the abutment movements in the mesial-distal, bucco-lingual and occluso-gingival directions all had statistical significance (P0.05). The largest mesial-distal inclination occurred with the Mini-SG attachment. The Ceka attachment produced more abutment displacement in mesial distal and apical directions. Occlusal displacement and anti-clockwise rotation were the least with the Snap attachment.The most equitable distribution of stresses on the supporting structures was achieved by Snap attachment with its resilent assemble, Ceka attachment transfers most of the loading forces to the abutments, Mini-SG attachment can not reduce the excessive torque applied to the abutments effectively.