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To investigated the role of HIF-1α in myocardial inflammatory injury in rats induced by CME and its possible mechanism. Forty SD rats were separated randomly and equally into four groups, i.e. CME+HIF-1α stabilizer dimethyloxalyl glycine (CME+DMOG) group, CME+HIF-1α inhibitor YC-1 (CME+YC-1) group, CME group, and Sham group. HBFP staining, myocardial enzyme assessment, and cardiac ultrasound were used to measure microinfarct, serum c-troponin I (cTnI) level, and Cardiac function. ELISA and western blot were applied for detecting NLRP3 inflammasome pathway and TLR4/MyD88/NF-κB signaling level.Pro-inflammatory factors of IL-18, IL-1β and TNF-α increased their expression levels after CME, which indicated inflammatory responses in the myocardium. Additionally, in the inflammatory process, NLRP3 inflammasome and TLR4/MyD88/NF-κB signaling were involved. DMOG reverses these effects of CME, whereas YC-1 aggravates these effects. HIF-1α may attenuate myocardial inflammatory injury induced by CME and improve cardiac function, which can perhaps be explained by the fact that TLR4/MyD88/NF-κB signaling pathway activation is inhibited.

The relationships between inflow and outflow water quality data for Poyang Lake from 1996 to 2016 are discussed and the main influencing factors are identified. TN and TP were the main factors causing a decline in water quality in Poyang Lake during the study period. The water quality of both the inflow and outflow rivers was generally good between 1996 and 2003; however, water quality declined over the study period, which is attributed to an increase in nutrients loads in the watershed. From 2004 to 2011, the water quality of the "Five Rivers" decreased significantly, which caused the water quality of Poyang Lake to decline. Due to the high purification capacity of Poyang Lake, the water quality of the outflow during this period was relatively good. A decline in water quality after this point was affected by pollution loads and hydrological conditions. Specifically, from 2012 to 2016, water quality in Poyang Lake and of the inflow water declined further. This was combined with a decrease in the water-purification capacity of the lake due to changes in the hydrological conditions, resulting in lower water quality at the outflow. Overall, the water quality of the inflow river has been closely related to the water quality in Poyang Lake. The concentrations of TN were significantly higher in the southern and eastern areas of Poyang Lake compared to the western areas. Higher nutrient loading from the Ganjiang River and the Xinjiang River has been an important driver. The concentrations of TP in the southern area of the lake have been significantly higher than in the eastern and western areas. This is attributed to comparatively high TP loads in the Ganjiang River and the Fuhe River. Compared to the changes in hydrological conditions, variations in nutrient loading have had a greater effect on water quality in the lake.

Background: Genome-wide association studies (GWAS) have recently shown that Single Nucleotide Polymorphism (SNP) rs17465637 on chromosome 1p41 is associated with atherothrombotic Coronary Artery Disease (CAD). However, whether rs17465637 acts as a protective factor or a risk factor for acute myocardial infarction (AMI) is not well understood in the general population. Objectives: In this article, we aimed to determine whether this locus was related to susceptibility to AMI in a Chinese Han population. Methods: A retrospective experimental study was performed in Guangxi province, People’s Republic of China, on January 1, 2012, to December 31, 2017. We recruited 688 patients who were matched for age, lifestyle, and socioeconomic status from the Chinese Han population and subdivided them into two groups of 344 AMI patients and 344 healthy controls. We used standardized questionnaires to collect information on demographics, socioeconomic status, and lifestyle factors. Genotypes of SNP rs17465637 were determined by the TaqMan assay. Diagnostic criteria and research protocols were based on the guidelines of the European Resuscitation Commission. Statistical analysis was performed by SPSS version 22.0. Results: The percentage of the AA genotype in the AMI group was 22.97%, which was greater than that of the control group (13.08%) (kappa = -0.082, P < 0.001). The AA genotype of SNP rs17465637 had significant differences between different infarct sites (kappa = -0.011, P < 0.05). There were interactions between the CC genotype and BMI ≥ 24 kg/m2 (OR = 4.060, 95% CI = 1.680 - 9.812, P = 0.002) and smoking ≥ 20 cigarettes/d (OR = 2.732, 95% CI = 1.495 - 4.991, P = 0.001). Conclusions: This study revealed that the AA genotype of SNP rs17465637 was positively correlated with the risk of AMI. Subjects with the AA genotype were positively correlated with extensive anterior of AMI. Also, interactions between the CC genotype of SNP rs17465637 and BMI or smoking seem to increase the risk of AMI.

Background: Large-scale population studies showed that the SNP rs1764391 of Connexin37 gene also known as Cx37 gene may play a pivotal role in the occurrence and development of acute myocardial infarction (AMI). Published results, however, are highly controversial. Objective: This study aimed to examine the association between SNP rs1764391 of Cx37 and diseasesusceptibility, several risk factors, and gene-environment interactions of AMI in Guangxi Han Chinese population. Methods: In this study, 344 healthy controls and 344 AMI patients of Han Chinese population were enrolled. The TaqMan assay was implemented to identify genotypes of Cx37 and allele frequencies of SNP rs1764391 in both the AMI and control groups. Results: Significant differences were detected in TT genotype frequencies of SNP rs1764391 between the AMI and control groups (P < 0.05). In the context of gender stratification, the result was also statistically different in women (P < 0.05). Each variable such as age, BMI, diabetes, high blood pressure, smoking and TC was a risk factor and correlated significantly (P < 0.05) with the development of AMI. HDL-C correlated negatively with the risk of AMI (P < 0.001). BMI, smoking or alcohol consumed interacts significantly (P < 0.017) with the presence of the SNP rs1764391 CC genotype. Conclusions: Evidences were presented that Cx37 rs1764391 variation may contribute to the risk for AMI, especially in women and this genetic variant may prove to be a potential biomarker for AMI risk stratification and may prove to be a useful target for therapeutic intervention to further improve prognosis in high-risk patients.

OBJECTIVE The correlation of the BRG1 rs1122608 single nucleotide polymorphism (SNP) with acute myocardial infarction (AMI) has been reported in American and European populations. However, whether rs1122608 acts as a protective factor or a risk factor for AMI is controversial. In this study, we aimed to detect the associations between rs1122608 and the clinical characteristics of AMI as well as susceptibility, gene-environment interactions, and risk factors for AMI in a Chinese Han population. METHODS In this study, 300 AMI patients and 300 healthy controls of Chinese Han ancestry were enrolled. PCR-RFLP was used to genotype rs1122608 SNPs. Genotypic and allelic frequencies of rs1122608 were compared between the AMI and control groups and among four AMI subgroups, which were subdivided by typical symptom, diagnosis time (DT), infarction location andserious complication. RESULTS Significant differences were detected between the AMI patients and the controls in both the genotypic and allelic frequencies of rs1122608 (p

According to the characteristics of the tea product traceability and monitoring system, the master-slavedatabase architecture is adopted. Based on Amoeba frame, the separation technology of reading and writing isachieved. Experimental results show that the design of master-slave distributed database is correct. And the achievement of separation technology of reading and writing effectively implement a distributed data managementto improve data processing efficiency.

Poly (lactide-co-glycolide) (PLGA, LA/GA = 80/20) with different L-lactide and D-lactide molar ratio (L-LA/D-LA = 1.0∼0.5) has been synthesized, and electrospun into nanofibrous membranes. The obtained copolymers were characterized by 1HNMR, Polarimeter, GPC and DSC. The relationship between the contraction of the membranes and the L-LA/D-LA ratio of copolymers has been investigated through DSC, SEM and XRD. The results indicated that with the L-LA/D-LA ratio decreasing, the crystal fragments in copolymer decreased significantly even disappeared; meanwhile, the contraction of the membranes in PBS at 37 °C increased. The mechanism of the contraction of electrospun PLGA membrane during incubated is proposed based on the present results.

Premise of the study: Microsatellite markers were developed in the invasive species Bidens alba (Asteraceae) to assess its population structure and to facilitate tracking its expansion in China. Methods and Results: Using 454 pyrosequencing, 20 microsatellite primer sets were developed for B. alba. The markers were tested on one population of B. alba (30 individuals) and one population of the closely related B. pilosa (30 individuals) in China. For B. alba, all of the markers were polymorphic, and the number of alleles per locus ranged from three to 32. The expected heterozygosity values were from 0.3787 to 0.9284, and the Shannon–Wiener index was from 0.6796 to 2.8401. Conclusions: These markers will be useful for investigating the genetic structure, genetic diversity, and invasion dynamics of B. alba and will also be useful in studies of B. pilosa.